Rh5BG060200

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008048
Physical Location & Seq
Forward (+)
4936498 .. 4959514
23017 bp
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UTR
Exon/CDS
Intron
Rh5BG060200.1

Sequence Viewer

Length: 612 bp
ATGATCACCAGCTGGAGGATCTTCACCTTTCTGCTCAGGCTACTAGCCTTGGCTGCAACTCTGGTTGCCACCATTGTAATGGTTACAAGCCATGACTCTACAAATGTCTTGAACTTAACGTTCAAGGCCAAATTCAGCAACTCACCAGCTTTCAAGTACTTTGTGGTTGTGGAAGCAATTGCCTGCGCGTACAACCTTGTCATTATGTTTCTTGCTTCCAAGGGCTCGCTTTGGCGCTTGGTTATCATCCTCGACGTGGTTGTAGCGGTGTTACTTGCTTCAAGTGTGTCATCGGCCTTGGCAATAGCATATGTGGGGAAGAAAGGAAACACGCACGCTGGTTGGCTTCCGATTTGTGGACAAGTCCCAAAGTTCTGCAAGCATGTCACTGCTGCTCTTGTTGCTGGCTTCATTGCAGCAATCCTGTATTTTCTTCTTGTTATCTATACCCTCTACACTGTTCTCCATCCTCTATTTGTTGTAAAAATGGCTCTCGCTGTTACTTCTTCTTCGTCTTCTACTGCAATCTCTACTCGGACCTTCTCCTCTGACCTCAAAGGTGATGATTATTGTAAACCGATTAGGTCTTTATCAGGAGCTGGATGGGTATAA

Protein Analysis

203

Amino Acids

21.89

Weight (kDa)

9.66

Isoelectric Point (pI)

26.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 4 - 142 4e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 188
AciI CCGC 1 cut(s) 266
AclI AACGTT 1 cut(s) 119
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 2 cut(s) 158, 191
AfiI CCNNNNNNNGG 3 cut(s) 15, 256, 356
AgsI TTSAA 4 cut(s) 112, 124, 154, 282
AjiI CACGTC 1 cut(s) 256
AluBI AGCT 3 cut(s) 12, 149, 599
AluI AGCT 3 cut(s) 12, 149, 599
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 599
AoxI GGCC 2 cut(s) 126, 294
ApeKI GCWGC 3 cut(s) 53, 392, 416
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 2 cut(s) 188, 237
AspS9I GGNCC 1 cut(s) 537
AsuHPI GGTGA 3 cut(s) 16, 135, 572
AvaII GGWCC 1 cut(s) 537
BanII GRGCYC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 507
BbvI GCAGC 3 cut(s) 40, 379, 428
BccI CCATC 2 cut(s) 474, 597
BclI TGATCA 1 cut(s) 3
BfaI CTAG 1 cut(s) 44
BfoI RGCGCY 1 cut(s) 238
BisI GCNGC 3 cut(s) 54, 393, 417
BlsI GCNGC 3 cut(s) 55, 394, 418
BmcAI AGTACT 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 537
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 1 cut(s) 537
BpiI GAAGAC 1 cut(s) 507
BpmI CTGGAG 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 35
BsaJI CCNNGG 3 cut(s) 48, 219, 297
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 256, 356
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 3 cut(s) 48, 219, 297
BseGI GGATG 3 cut(s) 246, 466, 608
BseLI CCNNNNNNNGG 3 cut(s) 15, 256, 356
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 49
BseRI GAGGAG 1 cut(s) 535
BseXI GCAGC 3 cut(s) 40, 379, 428
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 2 cut(s) 128, 296
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 15, 256, 356
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 2 cut(s) 128, 296
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 2 cut(s) 3, 18
BspACI CCGC 1 cut(s) 266
BspANI GGCC 2 cut(s) 128, 296
BspCNI CTCAG 1 cut(s) 48
BspFNI CGCG 1 cut(s) 188
BspPI GGATC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 411
BssECI CCNNGG 3 cut(s) 48, 219, 297
BssMI GATC 2 cut(s) 3, 18
BssT1I CCWWGG 3 cut(s) 48, 219, 297
Bst4CI ACNGT 1 cut(s) 460
BstC8I GCNNGC 5 cut(s) 184, 227, 336, 380, 406
BstDEI CTNAG 1 cut(s) 35
BstF5I GGATG 3 cut(s) 246, 466, 608
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 2 cut(s) 188, 237
BstKTI GATC 2 cut(s) 6, 21
BstMBI GATC 2 cut(s) 3, 18
BstMWI GCNNNNNNNGC 2 cut(s) 53, 401
BstNSI RCATGY 1 cut(s) 386
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 3 cut(s) 40, 379, 428
BstV2I GAAGAC 1 cut(s) 507
BstX2I RGATCY 1 cut(s) 18
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 2 cut(s) 128, 296
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 3 cut(s) 246, 466, 608
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 387, 456
Cac8I GCNNGC 5 cut(s) 184, 227, 336, 380, 406
CaiI CAGNNNCTG 1 cut(s) 599
CfoI GCGC 2 cut(s) 188, 237
Cfr13I GGNCC 1 cut(s) 537
Csp6I GTAC 2 cut(s) 157, 190
CviAII CATG 2 cut(s) 92, 383
CviQI GTAC 2 cut(s) 157, 190
DdeI CTNAG 1 cut(s) 35
DpnI GATC 2 cut(s) 5, 20
DpnII GATC 2 cut(s) 3, 18
Eco130I CCWWGG 3 cut(s) 48, 219, 297
Eco24I GRGCYC 1 cut(s) 227
Eco47I GGWCC 1 cut(s) 537
EcoT14I CCWWGG 3 cut(s) 48, 219, 297
EcoT38I GRGCYC 1 cut(s) 227
ErhI CCWWGG 3 cut(s) 48, 219, 297
FaeI CATG 2 cut(s) 95, 386
FaiI YATR 7 cut(s) 93, 206, 310, 312, 384, 447, 610
FaqI GGGAC 1 cut(s) 350
FatI CATG 2 cut(s) 91, 382
FauNDI CATATG 1 cut(s) 310
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 54, 393, 417
FokI GGATG 2 cut(s) 233, 453
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 3 cut(s) 54, 393, 417
FspBI CTAG 1 cut(s) 44
GlaI GCGC 2 cut(s) 187, 236
GluI GCNGC 3 cut(s) 54, 393, 417
GsuI CTGGAG 1 cut(s) 34
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 2 cut(s) 128, 296
HhaI GCGC 2 cut(s) 188, 237
Hin1II CATG 2 cut(s) 95, 386
Hin6I GCGC 2 cut(s) 186, 235
HinP1I GCGC 2 cut(s) 186, 235
HinfI GANTC 1 cut(s) 95
HphI GGTGA 3 cut(s) 16, 135, 572
Hpy166II GTNNAC 2 cut(s) 359, 575
Hpy188I TCNGA 3 cut(s) 351, 537, 550
Hpy188III TCNNGA 2 cut(s) 109, 594
Hpy8I GTNNAC 2 cut(s) 359, 575
Hpy99I CGWCG 1 cut(s) 257
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 2 cut(s) 119, 255
HpyCH4V TGCA 4 cut(s) 56, 378, 416, 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 401
HpyF3I CTNAG 1 cut(s) 35
HpySE526I ACGT 2 cut(s) 119, 255
Hsp92II CATG 2 cut(s) 95, 386
HspAI GCGC 2 cut(s) 186, 235
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 18
LmnI GCTCC 1 cut(s) 596
Lsp1109I GCAGC 3 cut(s) 40, 379, 428
MaeI CTAG 1 cut(s) 44
MaeII ACGT 2 cut(s) 119, 255
MaeIII GTNAC 4 cut(s) 82, 270, 385, 499
MalI GATC 2 cut(s) 5, 20
MboI GATC 2 cut(s) 3, 18
MboII GAAGA 6 cut(s) 13, 331, 425, 498, 501, 507
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 2 cut(s) 131, 177
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 6 cut(s) 9, 260, 461, 480, 556, 563
MseI TTAA 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 77
MspA1I CMGCKG 1 cut(s) 12
MunI CAATTG 1 cut(s) 177
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 53, 401
NdeI CATATG 1 cut(s) 310
NdeII GATC 2 cut(s) 3, 18
NlaIII CATG 2 cut(s) 95, 386
NmuCI GTSAC 1 cut(s) 385
NspI RCATGY 1 cut(s) 386
PkrI GCNGC 3 cut(s) 55, 394, 418
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 119
PspPI GGNCC 1 cut(s) 537
PstNI CAGNNNCTG 1 cut(s) 599
PsuI RGATCY 1 cut(s) 18
PvuII CAGCTG 1 cut(s) 12
RsaI GTAC 2 cut(s) 158, 191
RsaNI GTAC 2 cut(s) 157, 190
RseI CAYNNNNRTG 1 cut(s) 77
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 3 cut(s) 54, 393, 417
Sau3AI GATC 2 cut(s) 3, 18
Sau96I GGNCC 1 cut(s) 537
ScaI AGTACT 1 cut(s) 158
SchI GAGTC 1 cut(s) 89
SduI GDGCHC 1 cut(s) 227
SinI GGWCC 1 cut(s) 537
SmiMI CAYNNNNRTG 1 cut(s) 77
Sse9I AATT 2 cut(s) 131, 177
SsiI CCGC 1 cut(s) 266
SspMI CTAG 1 cut(s) 44
StyI CCWWGG 3 cut(s) 48, 219, 297
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 2 cut(s) 122, 258
TaqI TCGA 1 cut(s) 252
TasI AATT 2 cut(s) 131, 177
TatI WGTACW 1 cut(s) 156
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 2 cut(s) 394, 463
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 3 cut(s) 53, 392, 416
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 1 cut(s) 400
TspRI CASTG 2 cut(s) 394, 463
VpaK11BI GGWCC 1 cut(s) 537
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XspI CTAG 1 cut(s) 44
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.