FvH4_3g09260

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008048
Physical Location & Seq
Reverse (-)
5417476 .. 5418703
1228 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09260.t1

Sequence Viewer

Length: 492 bp
ATGATCACCGGCAGGAGGATCTTCACCTTTCTGCTTAGGCTGCTGACCTTTGCTGCAACTCTGGTTGCCACCATTGTGATGGTTACAAGCCATGACTCTACTAATGTCTTGAACTTAACATTCAAGGCCAAATTCAGCAACGCACCAGCTTTCAAGTACTTTGTGGTTGTGGAAGCAATTGCTTGCGCGTACAGCTTAGTCATTATGTTTCTTTCTTCCAAGGGCTCGCTTTGGCGCTTGGTTATCATCCTCGACGTGGTTGTAGCTGTGTTACTTGCATCAAGTGTGTCATCGGCACTGGCAATAGCAAGTGTGGGGAAGAAAGGAAACATGCACGCTGGTTGGCTTCCGATTTGTGGACAAGTCCCCAAGTTTTGCAACCATGTCACTGCTGCTCTTGTTGCTGGCTTCGTTGCAGCAATCCTGTATTTTCTTCTTGTTATCTATACCCTCTACACTGTTCTCCATCCTCTATTTGTTGTAAAATCTTGA

Protein Analysis

164

Amino Acids

17.64

Weight (kDa)

9.84

Isoelectric Point (pI)

28.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 5 - 142 4.8e-40 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 188
AclWI GGATC 1 cut(s) 26
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 2 cut(s) 158, 191
AfiI CCNNNNNNNGG 3 cut(s) 15, 256, 356
AgsI TTSAA 3 cut(s) 112, 124, 154
AjiI CACGTC 1 cut(s) 256
AleI CACNNNNGTG 1 cut(s) 74
AluBI AGCT 3 cut(s) 149, 195, 266
AluI AGCT 3 cut(s) 149, 195, 266
AlwI GGATC 1 cut(s) 26
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 4 cut(s) 40, 53, 392, 416
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 2 cut(s) 188, 237
AsuHPI GGTGA 1 cut(s) 16
BanII GRGCYC 1 cut(s) 227
BbvI GCAGC 4 cut(s) 27, 40, 379, 428
BccI CCATC 2 cut(s) 73, 474
BclI TGATCA 1 cut(s) 3
BfoI RGCGCY 1 cut(s) 238
BisI GCNGC 4 cut(s) 41, 54, 393, 417
BlsI GCNGC 4 cut(s) 42, 55, 394, 418
BmcAI AGTACT 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 256
BmsI GCATC 1 cut(s) 287
Bpu10I CCTNAGC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 219
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 256, 356
Bse118I RCCGGY 1 cut(s) 8
Bse1I ACTGG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 2 cut(s) 246, 466
BseLI CCNNNNNNNGG 3 cut(s) 15, 256, 356
BseNI ACTGG 1 cut(s) 303
BseXI GCAGC 4 cut(s) 27, 40, 379, 428
Bsh1236I CGCG 1 cut(s) 188
BshFI GGCC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 350
BslI CCNNNNNNNGG 3 cut(s) 15, 256, 356
BsmFI GGGAC 1 cut(s) 350
BsnI GGCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 2 cut(s) 3, 18
BspANI GGCC 1 cut(s) 128
BspFNI CGCG 1 cut(s) 188
BspPI GGATC 1 cut(s) 26
BsrFI RCCGGY 1 cut(s) 8
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 2 cut(s) 3, 18
BssT1I CCWWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 460
BstC8I GCNNGC 4 cut(s) 184, 227, 336, 406
BstDEI CTNAG 2 cut(s) 35, 196
BstF5I GGATG 2 cut(s) 246, 466
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 238
BstHHI GCGC 2 cut(s) 188, 237
BstKTI GATC 2 cut(s) 6, 21
BstMBI GATC 2 cut(s) 3, 18
BstMWI GCNNNNNNNGC 3 cut(s) 40, 192, 401
BstNSI RCATGY 1 cut(s) 334
BstUI CGCG 1 cut(s) 188
BstV1I GCAGC 4 cut(s) 27, 40, 379, 428
BstX2I RGATCY 1 cut(s) 18
BstXI CCANNNNNNTGG 1 cut(s) 79
BstYI RGATCY 1 cut(s) 18
BsuRI GGCC 1 cut(s) 128
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 2 cut(s) 246, 466
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 3 cut(s) 296, 387, 456
Cac8I GCNNGC 4 cut(s) 184, 227, 336, 406
CfoI GCGC 2 cut(s) 188, 237
Cfr10I RCCGGY 1 cut(s) 8
Csp6I GTAC 2 cut(s) 157, 190
CviAII CATG 3 cut(s) 92, 331, 383
CviJI RGCY 9 cut(s) 40, 90, 128, 149, 195, 225, 266, 346, 408
CviKI_1 RGCY 9 cut(s) 40, 90, 128, 149, 195, 225, 266, 346, 408
CviQI GTAC 2 cut(s) 157, 190
DdeI CTNAG 2 cut(s) 35, 196
DpnI GATC 2 cut(s) 5, 20
DpnII GATC 2 cut(s) 3, 18
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 227
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 3 cut(s) 95, 334, 386
FaiI YATR 5 cut(s) 93, 206, 332, 384, 447
FaqI GGGAC 1 cut(s) 350
FatI CATG 3 cut(s) 91, 330, 382
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 4 cut(s) 41, 54, 393, 417
FokI GGATG 2 cut(s) 233, 453
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 4 cut(s) 41, 54, 393, 417
GlaI GCGC 2 cut(s) 187, 236
GluI GCNGC 4 cut(s) 41, 54, 393, 417
HaeII RGCGCY 1 cut(s) 238
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 9
HhaI GCGC 2 cut(s) 188, 237
Hin1II CATG 3 cut(s) 95, 334, 386
Hin6I GCGC 2 cut(s) 186, 235
HinP1I GCGC 2 cut(s) 186, 235
HinfI GANTC 1 cut(s) 95
HpaII CCGG 1 cut(s) 9
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 359
Hpy188I TCNGA 1 cut(s) 351
Hpy188III TCNNGA 2 cut(s) 109, 489
Hpy8I GTNNAC 1 cut(s) 359
Hpy99I CGWCG 1 cut(s) 257
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 5 cut(s) 56, 278, 334, 378, 416
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 192, 401
HpyF3I CTNAG 2 cut(s) 35, 196
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 3 cut(s) 95, 334, 386
HspAI GCGC 2 cut(s) 186, 235
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 18
LpnPI CCDG 7 cut(s) 22, 47, 159, 284, 324, 390, 437
Lsp1109I GCAGC 4 cut(s) 27, 40, 379, 428
LweI GCATC 1 cut(s) 287
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 82, 270, 385
MalI GATC 2 cut(s) 5, 20
MboI GATC 2 cut(s) 3, 18
MboII GAAGA 4 cut(s) 13, 207, 331, 425
MfeI CAATTG 1 cut(s) 177
MflI RGATCY 1 cut(s) 18
MhlI GDGCHC 1 cut(s) 227
MluCI AATT 2 cut(s) 131, 177
MlyI GAGTC 1 cut(s) 89
MnlI CCTC 4 cut(s) 9, 260, 461, 480
MseI TTAA 1 cut(s) 116
MslI CAYNNNNRTG 2 cut(s) 74, 77
MspI CCGG 1 cut(s) 9
MunI CAATTG 1 cut(s) 177
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 3 cut(s) 40, 192, 401
NdeII GATC 2 cut(s) 3, 18
NlaIII CATG 3 cut(s) 95, 334, 386
NmuCI GTSAC 1 cut(s) 385
NspI RCATGY 1 cut(s) 334
OliI CACNNNNGTG 1 cut(s) 74
PkrI GCNGC 4 cut(s) 42, 55, 394, 418
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 2 cut(s) 158, 191
RsaNI GTAC 2 cut(s) 157, 190
RseI CAYNNNNRTG 2 cut(s) 74, 77
SaqAI TTAA 1 cut(s) 116
SatI GCNGC 4 cut(s) 41, 54, 393, 417
Sau3AI GATC 2 cut(s) 3, 18
ScaI AGTACT 1 cut(s) 158
SchI GAGTC 1 cut(s) 89
SduI GDGCHC 1 cut(s) 227
SetI ASST 6 cut(s) 29, 50, 151, 197, 258, 268
SfaNI GCATC 1 cut(s) 287
SmiMI CAYNNNNRTG 2 cut(s) 74, 77
Sse9I AATT 2 cut(s) 131, 177
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 1 cut(s) 258
TaqI TCGA 1 cut(s) 252
TasI AATT 2 cut(s) 131, 177
TatI WGTACW 1 cut(s) 156
Tru1I TTAA 1 cut(s) 116
Tru9I TTAA 1 cut(s) 116
TscAI CASTG 3 cut(s) 303, 394, 463
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 4 cut(s) 40, 53, 392, 416
Tsp45I GTSAC 1 cut(s) 385
TspRI CASTG 3 cut(s) 303, 394, 463
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
ZrmI AGTACT 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.