AT1G04940

Protein TIC 20-I, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007156
Physical Location & Seq
Forward (+)
1399182 .. 1400830
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04940.1

Sequence Viewer

Length: 825 bp
ATGATAACTGGATACAGCACGCCAAGTGCACATGTTCTAATGAGCTCTCGGGCATTCAAGTCATCATCATATAGAGCTGCAGCAGGACAGACTCAACATTATCTTGCTCGAAGTTCATTGCCTGTCGTAAAGAACTCGTGGGGATCACCACCTTCACCTTTCAATGAGCTTCCGAGAGTGTCAAGAGGTGTGCCTCTGTCATATCTCTCAGCCTCGTCTTCTCTGCTTCTGAATGGAGAACAAGGTAGTCTATCTGGTACATTACCTGTGTTACCTGTCCGCAGAAAAACTCTTTTGACTCCACGAGCGTCAAAAGATGTACCTTCTAGCTTCCGATTTCCCCCGATGACCAAGAAGCCACAATGGTGGTGGAGAACTTTGGCTTGCCTGCCTTACCTAATGCCACTGCATGAAACTTGGATGTATGCAGAAACCGCTTACCATCTCCACCCATTCCTAGAAGATTTTGAATTCTTAACCTACCCATTTCTAGGCGCCATAGGAAGATTACCAAGCTGGTTCCTCATGGCTTACTTTTTTGTAGCTTATCTAGGGATAGTGCGAAGAAAAGAATGGCCTCACTTCTTCAGGTTCCATGTAGTGATGGGTATGCTGCTTGAAATCGCACTCCAGGTTATAGGGACCGTTAGCAAGTGGATGCCTCTTGGAGTCTATTGGGGTAAGTTTGGGATGCATTTCTGGACTGCTGTTGCGTTTGCTTATCTGTTTACCGTCCTTGAAAGCATACGGTGTGCACTTGCGGGTATGTACGCAGACATCCCGTTTGTCTGTGATGCTGCCTATATCCAGATTCCGTACGACTAA

Protein Analysis

274

Amino Acids

31.2

Weight (kDa)

9.58

Isoelectric Point (pI)

52.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIC20 PF16166 93 - 267 7.9e-75 Chloroplast import apparatus Tic20-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 494
AciI CCGC 3 cut(s) 280, 435, 761
AclWI GGATC 1 cut(s) 151
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 571
AcyI GRCGYC 1 cut(s) 495
AfaI GTAC 4 cut(s) 259, 321, 770, 818
AfiI CCNNNNNNNGG 1 cut(s) 491
AflIII ACRYGT 1 cut(s) 31
AgsI TTSAA 5 cut(s) 58, 163, 470, 620, 740
AjnI CCWGG 1 cut(s) 630
AleI CACNNNNGTG 1 cut(s) 364
AluBI AGCT 6 cut(s) 45, 77, 169, 330, 516, 545
AluI AGCT 6 cut(s) 45, 77, 169, 330, 516, 545
Alw21I GWGCWC 3 cut(s) 31, 47, 757
Alw44I GTGCAC 2 cut(s) 27, 753
AlwI GGATC 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 48
AoxI GGCC 1 cut(s) 575
ApaLI GTGCAC 2 cut(s) 27, 753
ApeKI GCWGC 4 cut(s) 77, 80, 613, 797
ApoI RAATTY 1 cut(s) 470
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 1 cut(s) 642
AsuHPI GGTGA 2 cut(s) 138, 147
AvaI CYCGRG 1 cut(s) 48
AvaII GGWCC 1 cut(s) 642
BaeGI GKGCMC 2 cut(s) 31, 757
BanI GGYRCC 1 cut(s) 494
BanII GRGCYC 1 cut(s) 47
BauI CACGAG 2 cut(s) 136, 303
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 3 cut(s) 31, 47, 757
BbvI GCAGC 4 cut(s) 64, 92, 600, 784
BccI CCATC 2 cut(s) 450, 598
BciT130I CCWGG 1 cut(s) 632
BciVI GTATCC 1 cut(s) 5
BfaI CTAG 4 cut(s) 327, 458, 491, 551
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 498
BfuI GTATCC 1 cut(s) 5
BisI GCNGC 4 cut(s) 78, 81, 614, 798
BlsI GCNGC 4 cut(s) 79, 82, 615, 799
Bme1390I CCNGG 1 cut(s) 632
Bme18I GGWCC 1 cut(s) 642
BmeT110I CYCGRG 1 cut(s) 48
BmgT120I GGNCC 1 cut(s) 642
BmiI GGNNCC 4 cut(s) 496, 521, 593, 643
BmrFI CCNGG 1 cut(s) 632
BmsI GCATC 3 cut(s) 648, 681, 784
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 495
Bsc4I CCNNNNNNNGG 1 cut(s) 491
Bse1I ACTGG 1 cut(s) 13
Bse3DI GCAATG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 632
BseGI GGATG 4 cut(s) 426, 663, 696, 777
BseLI CCNNNNNNNGG 1 cut(s) 491
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 13
BseSI GKGCMC 2 cut(s) 31, 757
BseXI GCAGC 4 cut(s) 64, 92, 600, 784
BshFI GGCC 1 cut(s) 577
BshNI GGYRCC 1 cut(s) 494
BsiHKAI GWGCWC 3 cut(s) 31, 47, 757
BsiHKCI CYCGRG 1 cut(s) 48
BsiWI CGTACG 1 cut(s) 816
BslFI GGGAC 1 cut(s) 655
BslI CCNNNNNNNGG 1 cut(s) 491
BsmFI GGGAC 1 cut(s) 655
BsmI GAATGC 1 cut(s) 53
BsnI GGCC 1 cut(s) 577
BsoBI CYCGRG 1 cut(s) 48
Bsp1286I GDGCHC 3 cut(s) 31, 47, 757
Bsp143I GATC 1 cut(s) 143
BspACI CCGC 3 cut(s) 280, 435, 761
BspANI GGCC 1 cut(s) 577
BspCNI CTCAG 1 cut(s) 221
BspLI GGNNCC 4 cut(s) 496, 521, 593, 643
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 116
BsrI ACTGG 1 cut(s) 13
BssMI GATC 1 cut(s) 143
BssNI GRCGYC 1 cut(s) 495
BssSI CACGAG 2 cut(s) 136, 303
Bst2BI CACGAG 2 cut(s) 136, 303
Bst2UI CCWGG 1 cut(s) 632
Bst4CI ACNGT 3 cut(s) 646, 733, 750
BstACI GRCGYC 1 cut(s) 495
BstC8I GCNNGC 3 cut(s) 20, 385, 389
BstDEI CTNAG 1 cut(s) 208
BstF5I GGATG 4 cut(s) 426, 663, 696, 777
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 1 cut(s) 146
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 1 cut(s) 434
BstNI CCWGG 1 cut(s) 632
BstNSI RCATGY 1 cut(s) 35
BstSCI CCNGG 1 cut(s) 630
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 2 cut(s) 31, 757
BstV1I GCAGC 4 cut(s) 64, 92, 600, 784
BstV2I GAAGAC 1 cut(s) 210
BstXI CCANNNNNNTGG 1 cut(s) 366
BsuI GTATCC 1 cut(s) 5
BsuRI GGCC 1 cut(s) 577
BtsCI GGATG 4 cut(s) 426, 663, 696, 777
BtsI GCAGTG 1 cut(s) 404
BtsIMutI CAGTG 1 cut(s) 404
Cac8I GCNNGC 3 cut(s) 20, 385, 389
CfoI GCGC 1 cut(s) 497
Cfr13I GGNCC 1 cut(s) 642
CseI GACGC 1 cut(s) 297
Csp6I GTAC 4 cut(s) 258, 320, 769, 817
CspCI CAANNNNNGTGG 2 cut(s) 350, 385
CviAII CATG 4 cut(s) 32, 410, 526, 596
CviQI GTAC 4 cut(s) 258, 320, 769, 817
DdeI CTNAG 1 cut(s) 208
DinI GGCGCC 1 cut(s) 496
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 642
Eco53kI GAGCTC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 571
Eco88I CYCGRG 1 cut(s) 48
EcoICRI GAGCTC 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 630
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 47
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
FaeI CATG 4 cut(s) 35, 413, 529, 599
FaqI GGGAC 1 cut(s) 655
FatI CATG 4 cut(s) 31, 409, 525, 595
FauI CCCGC 1 cut(s) 754
Fnu4HI GCNGC 4 cut(s) 78, 81, 614, 798
FokI GGATG 4 cut(s) 433, 670, 703, 764
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 4 cut(s) 78, 81, 614, 798
FspBI CTAG 4 cut(s) 327, 458, 491, 551
GlaI GCGC 1 cut(s) 496
GluI GCNGC 4 cut(s) 78, 81, 614, 798
GsuI CTGGAG 1 cut(s) 614
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 1 cut(s) 577
HgaI GACGC 1 cut(s) 297
HhaI GCGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 495
Hin1II CATG 4 cut(s) 35, 413, 529, 599
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HinfI GANTC 4 cut(s) 91, 298, 669, 811
HphI GGTGA 2 cut(s) 138, 147
Hpy166II GTNNAC 3 cut(s) 29, 729, 755
Hpy188I TCNGA 3 cut(s) 174, 231, 335
Hpy188III TCNNGA 3 cut(s) 183, 700, 808
Hpy8I GTNNAC 3 cut(s) 29, 729, 755
HpyAV CCTTC 2 cut(s) 162, 333
HpyCH4III ACNGT 3 cut(s) 646, 733, 750
HpyCH4V TGCA 6 cut(s) 29, 80, 409, 428, 694, 755
HpyF10VI GCNNNNNNNGC 1 cut(s) 434
HpyF3I CTNAG 1 cut(s) 208
Hsp92I GRCGYC 1 cut(s) 495
Hsp92II CATG 4 cut(s) 35, 413, 529, 599
HspAI GCGC 1 cut(s) 495
KasI GGCGCC 1 cut(s) 494
Kzo9I GATC 1 cut(s) 143
Lsp1109I GCAGC 4 cut(s) 64, 92, 600, 784
LweI GCATC 3 cut(s) 648, 681, 784
MaeI CTAG 4 cut(s) 327, 458, 491, 551
MaeIII GTNAC 1 cut(s) 270
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 5 cut(s) 210, 473, 516, 576, 577
MhlI GDGCHC 3 cut(s) 31, 47, 757
MluCI AATT 1 cut(s) 470
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 3 cut(s) 85, 292, 678
MnlI CCTC 6 cut(s) 179, 204, 223, 533, 588, 672
Mph1103I ATGCAT 1 cut(s) 696
MseI TTAA 1 cut(s) 476
MslI CAYNNNNRTG 1 cut(s) 364
MspR9I CCNGG 1 cut(s) 632
Mva1269I GAATGC 1 cut(s) 53
MvaI CCWGG 1 cut(s) 632
MwoI GCNNNNNNNGC 1 cut(s) 434
NarI GGCGCC 1 cut(s) 495
NdeII GATC 1 cut(s) 143
NlaIII CATG 4 cut(s) 35, 413, 529, 599
NlaIV GGNNCC 4 cut(s) 496, 521, 593, 643
NsiI ATGCAT 1 cut(s) 696
NspI RCATGY 1 cut(s) 35
OliI CACNNNNGTG 1 cut(s) 364
PciI ACATGT 1 cut(s) 31
PctI GAATGC 1 cut(s) 53
PfeI GAWTC 1 cut(s) 811
Pfl23II CGTACG 1 cut(s) 816
PkrI GCNGC 4 cut(s) 79, 82, 615, 799
PleI GAGTC 3 cut(s) 85, 292, 677
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 3 cut(s) 85, 292, 677
PscI ACATGT 1 cut(s) 31
Psp124BI GAGCTC 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 630
PspGI CCWGG 1 cut(s) 630
PspLI CGTACG 1 cut(s) 816
PspN4I GGNNCC 4 cut(s) 496, 521, 593, 643
PspPI GGNCC 1 cut(s) 642
PstI CTGCAG 1 cut(s) 82
RsaI GTAC 4 cut(s) 259, 321, 770, 818
RsaNI GTAC 4 cut(s) 258, 320, 769, 817
RseI CAYNNNNRTG 1 cut(s) 364
SacI GAGCTC 1 cut(s) 47
SaqAI TTAA 1 cut(s) 476
SatI GCNGC 4 cut(s) 78, 81, 614, 798
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 1 cut(s) 642
SchI GAGTC 3 cut(s) 85, 292, 678
ScrFI CCNGG 1 cut(s) 632
SduI GDGCHC 3 cut(s) 31, 47, 757
SfaNI GCATC 3 cut(s) 648, 681, 784
SfcI CTRYAG 1 cut(s) 78
SfoI GGCGCC 1 cut(s) 496
SinI GGWCC 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 364
Sse9I AATT 1 cut(s) 470
SsiI CCGC 3 cut(s) 280, 435, 761
SspDI GGCGCC 1 cut(s) 494
SspMI CTAG 4 cut(s) 327, 458, 491, 551
SstI GAGCTC 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 630
TaaI ACNGT 3 cut(s) 646, 733, 750
TaqI TCGA 1 cut(s) 109
TasI AATT 1 cut(s) 470
TfiI GAWTC 1 cut(s) 811
Tru1I TTAA 1 cut(s) 476
Tru9I TTAA 1 cut(s) 476
TscAI CASTG 1 cut(s) 411
TseI GCWGC 4 cut(s) 77, 80, 613, 797
TspDTI ATGAA 2 cut(s) 105, 426
TspGWI ACGGA 1 cut(s) 804
TspRI CASTG 1 cut(s) 411
VneI GTGCAC 2 cut(s) 27, 753
VpaK11BI GGWCC 1 cut(s) 642
XapI RAATTY 1 cut(s) 470
XceI RCATGY 1 cut(s) 35
XcmI CCANNNNNNNNNTGG 1 cut(s) 366
XspI CTAG 4 cut(s) 327, 458, 491, 551
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.