AT1G06870

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009896
Physical Location & Seq
Forward (+)
2108512 .. 2110986
2475 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G06870.1

Sequence Viewer

Length: 1104 bp
ATGGCGATTAGGGTTACCTTCACCTACTCCAGCTATGTTGCTAGGAGCATTGCTTCATCCGCCGGGACTCGTGTCGGTACCGGCGACGTTAGATCATGCTTCGAAACATGGGTTCGTCCCAGGTTCTGCGGCCACAATCAGATACCAGATATTGTTGATAAATCTCCCGGATCCAACACATGGGGTCCAAGCTCAGGCCCTCGTGCTCGACCAGCTTCATCAATGTATAGCACCATTGCGAGGGAAATCCTCGAAGAAGGCTGCAAGAGTCCACTTGTCTTGGGTATGATCTCTCTCATGAATTTGACTGGAGCTCCACAGTTTTCGGGTATGACCGGTCTCGGGATCTCTCCCTTTAAGACTTCTTCTGTCATCCCGTTCCTTAGGGGTTCCAAGTGGATGCCTTGTAGTATTCCGGCGACGTTATCAACGGATATTGCTGAGGTTGATAGAGGAGGAAAGGTCTGTGATCCTAAAGTGAAGTTGGAGTTGAGTGATAAAGTCTCGAATGGTGGAAACGGATGGGTTAATAAGCTGTTGAATATCTGCTCGGAGGATGCTAAGGCTGCTTTCACGGCGGTTACTGTTTCTCTCCTTTTCCGATCGGCTTTGGCCGAGCCAAAGTCTATACCTTCAACATCTATGCTTCCTACTCTCGATGTGGGTGATCGTGTTATAGCCGAGAAGGTCTCATACTTTTTCAGGAAGCCAGAGGTTTCAGACATAGTTATCTTCAAGGCTCCTCCTATTTTGGTGGAACATGGTTACAGTTGTGCTGATGTTTTCATAAAAAGGATAGTTGCTAGCGAAGGTGACTGGGTTGAAGTTTGTGATGGAAAGCTCTTAGTAAATGACACTGTTCAAGCAGAGGATTTTGTCTTAGAGCCAATTGACTATGAAATGGAACCAATGTTTGTCCCTGAAGGTTATGTCTTCGTCCTAGGAGACAACCGCAACAAAAGCTTTGATTCTCATAACTGGGGTCCACTTCCAATAAAGAACATCATAGGGAGATCTGTGTTTCGCTATTGGCCACCAAGCAAAGTGTCAGACATAATACACCATGAACAAGTTAGCCAAAAGAGAGCTGTTGATGTATCTTGA

Protein Analysis

367

Amino Acids

40.15

Weight (kDa)

6.54

Isoelectric Point (pI)

35.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 187 - 344 5.4e-39 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 77
AccB1I GGYRCC 1 cut(s) 77
AccB7I CCANNNNNTGG 1 cut(s) 180
AciI CCGC 4 cut(s) 60, 129, 578, 952
AclWI GGATC 4 cut(s) 165, 178, 353, 464
AcoI YGGCCR 3 cut(s) 130, 612, 1031
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 942
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 4 cut(s) 180, 194, 240, 342
AgeI ACCGGT 1 cut(s) 335
AgsI TTSAA 5 cut(s) 541, 636, 736, 824, 863
AjnI CCWGG 1 cut(s) 119
AluBI AGCT 8 cut(s) 33, 192, 215, 314, 535, 841, 963, 1088
AluI AGCT 8 cut(s) 33, 192, 215, 314, 535, 841, 963, 1088
Alw21I GWGCWC 2 cut(s) 208, 316
Alw26I GTCTC 4 cut(s) 344, 508, 694, 939
AlwI GGATC 4 cut(s) 165, 178, 353, 464
AlwNI CAGNNNCTG 1 cut(s) 126
Ama87I CYCGRG 1 cut(s) 341
AoxI GGCC 4 cut(s) 130, 196, 612, 1031
ApeKI GCWGC 2 cut(s) 261, 566
ApoI RAATTY 1 cut(s) 301
AsiGI ACCGGT 1 cut(s) 335
Asp718I GGTACC 1 cut(s) 77
AspA2I CCTAGG 1 cut(s) 940
AspS9I GGNCC 3 cut(s) 185, 197, 983
AsuC2I CCSGG 2 cut(s) 64, 168
AsuHPI GGTGA 3 cut(s) 13, 677, 824
AsuII TTCGAA 1 cut(s) 102
AsuNHI GCTAGC 1 cut(s) 803
AvaI CYCGRG 1 cut(s) 341
AvaII GGWCC 2 cut(s) 185, 983
AvrII CCTAGG 1 cut(s) 940
AxyI CCTNAGG 1 cut(s) 383
BalI TGGCCA 1 cut(s) 1033
BamHI GGATCC 1 cut(s) 170
BanI GGYRCC 1 cut(s) 77
BanII GRGCYC 1 cut(s) 316
BarI GAAGNNNNNNTAC 2 cut(s) 677, 709
BauI CACGAG 2 cut(s) 69, 201
BbsI GAAGAC 1 cut(s) 925
Bbv12I GWGCWC 2 cut(s) 208, 316
BbvCI CCTCAGC 1 cut(s) 441
BbvI GCAGC 2 cut(s) 248, 553
BccI CCATC 2 cut(s) 516, 827
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 1 cut(s) 121
BcnI CCSGG 2 cut(s) 64, 168
BcoDI GTCTC 4 cut(s) 344, 508, 694, 939
BfaI CTAG 3 cut(s) 42, 804, 941
BglII AGATCT 1 cut(s) 1013
BisI GCNGC 3 cut(s) 130, 262, 567
BlnI CCTAGG 1 cut(s) 940
BlsI GCNGC 3 cut(s) 131, 263, 568
Bme1390I CCNGG 3 cut(s) 64, 121, 168
Bme18I GGWCC 2 cut(s) 185, 983
BmeT110I CYCGRG 1 cut(s) 341
BmgT120I GGNCC 3 cut(s) 185, 197, 983
BmiI GGNNCC 7 cut(s) 79, 172, 186, 391, 741, 906, 984
BmrFI CCNGG 3 cut(s) 64, 121, 168
BmrI ACTGGG 2 cut(s) 826, 988
BmsI GCATC 2 cut(s) 390, 547
BmtI GCTAGC 1 cut(s) 807
BmuI ACTGGG 2 cut(s) 826, 988
BoxI GACNNNNGTC 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 925
BplI GAGNNNNNCTC 2 cut(s) 674, 706
BpmI CTGGAG 2 cut(s) 13, 330
Bpu10I CCTNAGC 3 cut(s) 193, 441, 561
Bpu14I TTCGAA 1 cut(s) 102
BpuMI CCSGG 2 cut(s) 64, 168
BsaI GGTCTC 2 cut(s) 344, 694
BsaJI CCNNGG 2 cut(s) 119, 940
BsaWI WCCGGW 1 cut(s) 335
BsaXI ACNNNNNCTCC 4 cut(s) 298, 328, 447, 477
Bsc4I CCNNNNNNNGG 4 cut(s) 180, 194, 240, 342
Bse118I RCCGGY 2 cut(s) 80, 335
Bse1I ACTGG 3 cut(s) 313, 821, 983
Bse21I CCTNAGG 1 cut(s) 383
Bse3DI GCAATG 2 cut(s) 48, 234
BseBI CCWGG 1 cut(s) 121
BseDI CCNNGG 2 cut(s) 119, 940
BseGI GGATG 5 cut(s) 56, 372, 405, 527, 562
BseLI CCNNNNNNNGG 4 cut(s) 180, 194, 240, 342
BseMI GCAATG 2 cut(s) 48, 234
BseMII CTCAG 2 cut(s) 207, 432
BseNI ACTGG 3 cut(s) 313, 821, 983
BseRI GAGGAG 2 cut(s) 468, 732
BseXI GCAGC 2 cut(s) 248, 553
Bsh1285I CGRYCG 1 cut(s) 605
BshFI GGCC 4 cut(s) 132, 198, 614, 1033
BshNI GGYRCC 1 cut(s) 77
BshTI ACCGGT 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 605
BsiHKAI GWGCWC 2 cut(s) 208, 316
BsiHKCI CYCGRG 1 cut(s) 341
BsiSI CCGG 5 cut(s) 63, 81, 168, 336, 416
BslFI GGGAC 3 cut(s) 79, 102, 902
BslI CCNNNNNNNGG 4 cut(s) 180, 194, 240, 342
BsmAI GTCTC 4 cut(s) 344, 508, 694, 939
BsmFI GGGAC 3 cut(s) 79, 102, 902
BsnI GGCC 4 cut(s) 132, 198, 614, 1033
Bso31I GGTCTC 2 cut(s) 344, 694
BsoBI CYCGRG 1 cut(s) 341
Bsp119I TTCGAA 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 208, 316
Bsp143I GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
BspACI CCGC 4 cut(s) 60, 129, 578, 952
BspANI GGCC 4 cut(s) 132, 198, 614, 1033
BspCNI CTCAG 2 cut(s) 206, 433
BspHI TCATGA 1 cut(s) 297
BspLI GGNNCC 7 cut(s) 79, 172, 186, 391, 741, 906, 984
BspOI GCTAGC 1 cut(s) 807
BspPI GGATC 4 cut(s) 165, 178, 353, 464
BspT104I TTCGAA 1 cut(s) 102
BspT107I GGYRCC 1 cut(s) 77
BspTNI GGTCTC 2 cut(s) 344, 694
BsrDI GCAATG 2 cut(s) 48, 234
BsrFI RCCGGY 2 cut(s) 80, 335
BsrI ACTGG 3 cut(s) 313, 821, 983
BssAI RCCGGY 2 cut(s) 80, 335
BssECI CCNNGG 2 cut(s) 119, 940
BssMI GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
BssSI CACGAG 2 cut(s) 69, 201
BssT1I CCWWGG 1 cut(s) 940
Bst2BI CACGAG 2 cut(s) 69, 201
Bst2UI CCWGG 1 cut(s) 121
Bst4CI ACNGT 4 cut(s) 321, 586, 770, 859
BstBI TTCGAA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 805
BstDEI CTNAG 6 cut(s) 193, 383, 441, 561, 844, 880
BstEII GGTNACC 1 cut(s) 13
BstF5I GGATG 5 cut(s) 56, 372, 405, 527, 562
BstKTI GATC 8 cut(s) 95, 173, 291, 348, 472, 605, 670, 1016
BstMAI GTCTC 4 cut(s) 344, 508, 694, 939
BstMBI GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
BstMCI CGRYCG 1 cut(s) 605
BstMWI GCNNNNNNNGC 5 cut(s) 59, 212, 566, 575, 960
BstNI CCWGG 1 cut(s) 121
BstPAI GACNNNNGTC 1 cut(s) 71
BstPI GGTNACC 1 cut(s) 13
BstSCI CCNGG 3 cut(s) 62, 119, 166
BstV1I GCAGC 2 cut(s) 248, 553
BstV2I GAAGAC 1 cut(s) 925
BstX2I RGATCY 3 cut(s) 170, 345, 1013
BstYI RGATCY 3 cut(s) 170, 345, 1013
Bsu36I CCTNAGG 1 cut(s) 383
BsuRI GGCC 4 cut(s) 132, 198, 614, 1033
BtsCI GGATG 5 cut(s) 56, 372, 405, 527, 562
BtsIMutI CAGTG 1 cut(s) 855
Cac8I GCNNGC 1 cut(s) 805
CaiI CAGNNNCTG 1 cut(s) 126
CciI TCATGA 1 cut(s) 297
Cfr10I RCCGGY 2 cut(s) 80, 335
Cfr13I GGNCC 3 cut(s) 185, 197, 983
Csp6I GTAC 1 cut(s) 78
CspAI ACCGGT 1 cut(s) 335
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviAII CATG 6 cut(s) 96, 108, 180, 298, 761, 1064
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 6 cut(s) 193, 383, 441, 561, 844, 880
DpnI GATC 8 cut(s) 94, 172, 290, 347, 471, 604, 669, 1015
DpnII GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
EaeI YGGCCR 3 cut(s) 130, 612, 1031
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 940
Eco24I GRGCYC 1 cut(s) 316
Eco31I GGTCTC 2 cut(s) 344, 694
Eco47I GGWCC 2 cut(s) 185, 983
Eco53kI GAGCTC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 942
Eco81I CCTNAGG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 341
Eco91I GGTNACC 1 cut(s) 13
EcoICRI GAGCTC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 197
EcoO65I GGTNACC 1 cut(s) 13
EcoRII CCWGG 1 cut(s) 119
EcoT14I CCWWGG 1 cut(s) 940
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 940
FaeI CATG 6 cut(s) 99, 111, 183, 301, 764, 1067
FaqI GGGAC 3 cut(s) 79, 102, 902
FatI CATG 6 cut(s) 95, 107, 179, 297, 760, 1063
Fnu4HI GCNGC 3 cut(s) 130, 262, 567
FokI GGATG 5 cut(s) 43, 359, 412, 534, 569
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 3 cut(s) 130, 262, 567
FspBI CTAG 3 cut(s) 42, 804, 941
GluI GCNGC 3 cut(s) 130, 262, 567
GsuI CTGGAG 2 cut(s) 13, 330
HaeIII GGCC 4 cut(s) 132, 198, 614, 1033
HapII CCGG 5 cut(s) 63, 81, 168, 336, 416
Hin1II CATG 6 cut(s) 99, 111, 183, 301, 764, 1067
HindIII AAGCTT 1 cut(s) 961
HinfI GANTC 3 cut(s) 67, 268, 968
HpaII CCGG 5 cut(s) 63, 81, 168, 336, 416
HphI GGTGA 3 cut(s) 13, 677, 824
Hpy166II GTNNAC 2 cut(s) 272, 986
Hpy188I TCNGA 5 cut(s) 141, 553, 602, 721, 1051
Hpy188III TCNNGA 6 cut(s) 298, 343, 505, 656, 703, 1101
Hpy8I GTNNAC 2 cut(s) 272, 986
Hpy99I CGWCG 2 cut(s) 89, 424
HpyAV CCTTC 6 cut(s) 28, 251, 642, 679, 803, 917
HpyCH4III ACNGT 4 cut(s) 321, 586, 770, 859
HpyCH4IV ACGT 2 cut(s) 87, 422
HpyCH4V TGCA 1 cut(s) 264
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 212, 566, 575, 960
HpyF3I CTNAG 6 cut(s) 193, 383, 441, 561, 844, 880
HpySE526I ACGT 2 cut(s) 87, 422
Hsp92II CATG 6 cut(s) 99, 111, 183, 301, 764, 1067
KpnI GGTACC 1 cut(s) 81
Kzo9I GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
LmnI GCTCC 4 cut(s) 45, 311, 319, 745
Lsp1109I GCAGC 2 cut(s) 248, 553
LweI GCATC 2 cut(s) 390, 547
MaeI CTAG 3 cut(s) 42, 804, 941
MaeII ACGT 2 cut(s) 87, 422
MaeIII GTNAC 4 cut(s) 13, 580, 764, 812
MalI GATC 8 cut(s) 94, 172, 290, 347, 471, 604, 669, 1015
MboI GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
MboII GAAGA 4 cut(s) 266, 357, 724, 925
MfeI CAATTG 1 cut(s) 888
MflI RGATCY 3 cut(s) 170, 345, 1013
MhlI GDGCHC 2 cut(s) 208, 316
MlsI TGGCCA 1 cut(s) 1033
MluCI AATT 2 cut(s) 301, 888
MluNI TGGCCA 1 cut(s) 1033
MlyI GAGTC 2 cut(s) 61, 277
MmeI TCCRAC 2 cut(s) 198, 465
Mox20I TGGCCA 1 cut(s) 1033
MscI TGGCCA 1 cut(s) 1033
MseI TTAA 2 cut(s) 357, 528
Msp20I TGGCCA 1 cut(s) 1033
MspI CCGG 5 cut(s) 63, 81, 168, 336, 416
MspR9I CCNGG 3 cut(s) 64, 121, 168
MunI CAATTG 1 cut(s) 888
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 5 cut(s) 59, 212, 566, 575, 960
NciI CCSGG 2 cut(s) 64, 168
NdeII GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
NheI GCTAGC 1 cut(s) 803
NlaIII CATG 6 cut(s) 99, 111, 183, 301, 764, 1067
NlaIV GGNNCC 7 cut(s) 79, 172, 186, 391, 741, 906, 984
NmeAIII GCCGAG 2 cut(s) 640, 706
NmuCI GTSAC 1 cut(s) 812
NspV TTCGAA 1 cut(s) 102
PagI TCATGA 1 cut(s) 297
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PfeI GAWTC 1 cut(s) 968
PflMI CCANNNNNTGG 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 335
PkrI GCNGC 3 cut(s) 131, 263, 568
Ple19I CGATCG 1 cut(s) 605
PleI GAGTC 2 cut(s) 61, 276
PpsI GAGTC 2 cut(s) 61, 276
PshAI GACNNNNGTC 1 cut(s) 71
Psp124BI GAGCTC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 119
PspEI GGTNACC 1 cut(s) 13
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 7 cut(s) 79, 172, 186, 391, 741, 906, 984
PspPI GGNCC 3 cut(s) 185, 197, 983
PstNI CAGNNNCTG 1 cut(s) 126
PsuI RGATCY 3 cut(s) 170, 345, 1013
PvuI CGATCG 1 cut(s) 605
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SacI GAGCTC 1 cut(s) 316
SaqAI TTAA 2 cut(s) 357, 528
SatI GCNGC 3 cut(s) 130, 262, 567
Sau3AI GATC 8 cut(s) 92, 170, 288, 345, 469, 602, 667, 1013
Sau96I GGNCC 3 cut(s) 185, 197, 983
SchI GAGTC 2 cut(s) 61, 277
ScrFI CCNGG 3 cut(s) 64, 121, 168
SduI GDGCHC 2 cut(s) 208, 316
SfaNI GCATC 2 cut(s) 390, 547
SfuI TTCGAA 1 cut(s) 102
SinI GGWCC 2 cut(s) 185, 983
Sse9I AATT 2 cut(s) 301, 888
SsiI CCGC 4 cut(s) 60, 129, 578, 952
SspMI CTAG 3 cut(s) 42, 804, 941
SstI GAGCTC 1 cut(s) 316
StyD4I CCNGG 3 cut(s) 62, 119, 166
StyI CCWWGG 1 cut(s) 940
TaaI ACNGT 4 cut(s) 321, 586, 770, 859
TaiI ACGT 2 cut(s) 90, 425
TaqI TCGA 5 cut(s) 102, 208, 252, 506, 657
TasI AATT 2 cut(s) 301, 888
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 1 cut(s) 968
Tru1I TTAA 2 cut(s) 357, 528
Tru9I TTAA 2 cut(s) 357, 528
TscAI CASTG 1 cut(s) 862
TseFI GTSAC 1 cut(s) 812
TseI GCWGC 2 cut(s) 261, 566
Tsp45I GTSAC 1 cut(s) 812
TspDTI ATGAA 6 cut(s) 45, 207, 314, 775, 912, 1080
TspGWI ACGGA 2 cut(s) 446, 534
TspRI CASTG 1 cut(s) 862
Van91I CCANNNNNTGG 1 cut(s) 180
VpaK11BI GGWCC 2 cut(s) 185, 983
XapI RAATTY 1 cut(s) 301
XmaJI CCTAGG 1 cut(s) 940
XspI CTAG 3 cut(s) 42, 804, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.