MD15G1034800.v1.1

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009896
Physical Location & Seq
Forward (+)
2465849 .. 2468686
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1034800.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGGCTATCCGCGTAACCCTCAGCTTCTCCGGCTATGTGGCCCAAAACCTAGCATCCTCGGCAAGCCTCCGCGCGGGGAACTGCCGAGGATTTCAGGAATGCTGGGTTCGGTCTCGGGTATTCGGATCCAACCAGAAGCCCGATCTCGACCCCGCCGTCCCCGTCCGCAATTACCAGACCCAATTTTCCCGCTCGAAGCATTCGACGGCTGCCGTTAAGCCCTTGCCGTCATTGTACACCGCTCTCGCCGAGGAGATTCTGGGCGAGAGCTGCAAGAGCCCGATCGTGCTGGGCTTGATCTCGCTTCTGAAGTCAACGGCTATCGCCACCGGCGTCTCTTCGGCTCCGGCCTCTTTGGGGATTTCGCCGTTTAAATCCGGTTCGGTCATGCCCTTTTTACAGGTCTCAAAGTGGTTGCCCTGCAACGAGTCCGTCCCGGTGTCGATAATGAAGGAAGTGGATAAAGGTGGAACGTTGTGTGTCGATGACGTTGCAGAAGCCTCGCAGCTCTCCAAGAAAGACATGGGCAGGACCGGGTTTTTGTCGCGGTTGTTGAATTATTGCTCGGAGGACGCTAAGGCTGTGTTCACTGCTGTTACAGTCAGTGTATTGTTTAAGTCTTTCTTGGCTGAGCCCCGATCAATTCCCTCCACTTCAATGTACCCTACTCTCGATGTTGGCGATCGCGTTTTGGCGGAGAAGGTTTCATATTTCTTTAAAAAGCCTGAGGTTTCAGATATAGTAATTTTCAAGGCACCTCCAATCTTACAGGAAATCGGTTATAACTCAACCGATGTATTCATCAAAAGAATTGTAGCTAAGGCTGGAGATTGTGTTGAGGTCCGCGATGGGAAATTGCTGATCAATGGTCTTGTTCAGAATGAAAATTACATATTGGAGCCGCTCGCTTATGAAATGGATCCAGTGCTTATTCCAGAAGGCTATGTCTTCGTAATGGGAGATAATCGCAACAACAGCTTTGACTCTCATAATTGGGGTCCACTTCCTGTCAAAAACATTCTTGGCAGATCAGTATTTCGCTATTGGCCTCCCTCCAAGGTGTCCAACACCATGCTTGAACCACAAGGACCAAGTAACGCCGTTGCGATTTCTTGA

Protein Analysis

372

Amino Acids

40.39

Weight (kDa)

8.56

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 192 - 349 2.2e-43 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
AasI GACNNNNNNGTC 1 cut(s) 155
AccB1I GGYRCC 1 cut(s) 754
AccBSI CCGCTC 3 cut(s) 192, 242, 904
AccII CGCG 6 cut(s) 12, 72, 74, 547, 687, 846
AclI AACGTT 1 cut(s) 473
AclWI GGATC 4 cut(s) 120, 133, 914, 927
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 2 cut(s) 236, 662
AfiI CCNNNNNNNGG 2 cut(s) 73, 357
AgsI TTSAA 4 cut(s) 556, 657, 751, 1079
AluBI AGCT 5 cut(s) 24, 270, 508, 818, 978
AluI AGCT 5 cut(s) 24, 270, 508, 818, 978
Alw26I GTCTC 3 cut(s) 117, 340, 409
AlwI GGATC 4 cut(s) 120, 133, 914, 927
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 3 cut(s) 39, 348, 1046
ApeKI GCWGC 3 cut(s) 209, 270, 505
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
AsiSI GCGATCGC 1 cut(s) 685
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 5 cut(s) 40, 531, 841, 998, 1088
AsuC2I CCSGG 2 cut(s) 437, 535
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 4 cut(s) 531, 841, 998, 1088
AxyI CCTNAGG 1 cut(s) 726
BamHI GGATCC 2 cut(s) 125, 919
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 2 cut(s) 281, 636
BbsI GAAGAC 1 cut(s) 940
BbvCI CCTCAGC 1 cut(s) 20
BbvI GCAGC 3 cut(s) 196, 257, 517
BccI CCATC 1 cut(s) 842
BceAI ACGGC 7 cut(s) 140, 197, 211, 222, 333, 352, 1085
BcgI CGANNNNNNTGC 4 cut(s) 473, 483, 507, 517
BclI TGATCA 1 cut(s) 861
BcnI CCSGG 2 cut(s) 437, 535
BcoDI GTCTC 3 cut(s) 117, 340, 409
BfaI CTAG 1 cut(s) 50
BisI GCNGC 4 cut(s) 210, 271, 506, 902
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 4 cut(s) 211, 272, 507, 903
Bme1390I CCNGG 2 cut(s) 437, 535
Bme18I GGWCC 4 cut(s) 531, 841, 998, 1088
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 5 cut(s) 40, 531, 841, 998, 1088
BmiI GGNNCC 6 cut(s) 127, 345, 756, 900, 921, 999
BmrFI CCNGG 2 cut(s) 437, 535
BmsI GCATC 1 cut(s) 62
BpiI GAAGAC 1 cut(s) 940
BpmI CTGGAG 1 cut(s) 846
Bpu10I CCTNAGC 3 cut(s) 20, 576, 819
Bpu1102I GCTNAGC 1 cut(s) 630
BpuMI CCSGG 2 cut(s) 437, 535
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 117, 409
BsaJI CCNNGG 4 cut(s) 57, 85, 249, 1056
BsaWI WCCGGW 1 cut(s) 377
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 357
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 923
Bse21I CCTNAGG 1 cut(s) 726
BseDI CCNNGG 4 cut(s) 57, 85, 249, 1056
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 2 cut(s) 73, 357
BseMII CTCAG 3 cut(s) 34, 621, 717
BseNI ACTGG 1 cut(s) 923
BseRI GAGGAG 1 cut(s) 266
BseXI GCAGC 3 cut(s) 196, 257, 517
BseYI CCCAGC 2 cut(s) 102, 289
Bsh1236I CGCG 6 cut(s) 12, 72, 74, 547, 687, 846
Bsh1285I CGRYCG 2 cut(s) 285, 685
BshFI GGCC 3 cut(s) 41, 350, 1048
BshNI GGYRCC 1 cut(s) 754
BsiEI CGRYCG 2 cut(s) 285, 685
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 6 cut(s) 30, 330, 347, 378, 437, 534
BslFI GGGAC 2 cut(s) 143, 419
BslI CCNNNNNNNGG 2 cut(s) 73, 357
BsmAI GTCTC 3 cut(s) 117, 340, 409
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 143, 419
BsmI GAATGC 2 cut(s) 104, 199
BsnI GGCC 3 cut(s) 41, 350, 1048
Bso31I GGTCTC 2 cut(s) 117, 409
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 281, 636
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
Bsp1720I GCTNAGC 1 cut(s) 630
BspANI GGCC 3 cut(s) 41, 350, 1048
BspCNI CTCAG 3 cut(s) 33, 622, 718
BspFNI CGCG 6 cut(s) 12, 72, 74, 547, 687, 846
BspLI GGNNCC 6 cut(s) 127, 345, 756, 900, 921, 999
BspPI GGATC 4 cut(s) 120, 133, 914, 927
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 2 cut(s) 117, 409
BsrBI CCGCTC 3 cut(s) 192, 242, 904
BsrFI RCCGGY 1 cut(s) 329
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 1 cut(s) 923
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 4 cut(s) 57, 85, 249, 1056
BssMI GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 1 cut(s) 1056
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 1 cut(s) 343
BstACI GRCGYC 1 cut(s) 333
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 64, 906
BstDEI CTNAG 5 cut(s) 20, 576, 630, 726, 819
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 6 cut(s) 12, 72, 74, 547, 687, 846
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 9 cut(s) 128, 145, 285, 300, 641, 685, 864, 922, 1031
BstMAI GTCTC 3 cut(s) 117, 340, 409
BstMBI GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
BstMCI CGRYCG 2 cut(s) 285, 685
BstMWI GCNNNNNNNGC 5 cut(s) 30, 59, 270, 276, 975
BstSCI CCNGG 2 cut(s) 435, 533
BstUI CGCG 6 cut(s) 12, 72, 74, 547, 687, 846
BstV1I GCAGC 3 cut(s) 196, 257, 517
BstV2I GAAGAC 1 cut(s) 940
BstX2I RGATCY 2 cut(s) 125, 919
BstYI RGATCY 2 cut(s) 125, 919
Bsu36I CCTNAGG 1 cut(s) 726
BsuRI GGCC 3 cut(s) 41, 350, 1048
BtgZI GCGATG 1 cut(s) 861
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 3 cut(s) 588, 610, 930
Cac8I GCNNGC 2 cut(s) 64, 906
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 5 cut(s) 40, 531, 841, 998, 1088
CseI GACGC 2 cut(s) 322, 581
Csp6I GTAC 2 cut(s) 235, 661
CviAII CATG 3 cut(s) 388, 523, 1072
CviQI GTAC 2 cut(s) 235, 661
DdeI CTNAG 5 cut(s) 20, 576, 630, 726, 819
DpnI GATC 9 cut(s) 127, 144, 284, 299, 640, 684, 863, 921, 1030
DpnII GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
DraI TTTAAA 2 cut(s) 373, 718
DrdI GACNNNNNNGTC 1 cut(s) 155
DseDI GACNNNNNNGTC 1 cut(s) 155
Eam1104I CTCTTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 343
EciI GGCGGA 1 cut(s) 710
Eco130I CCWWGG 1 cut(s) 1056
Eco24I GRGCYC 2 cut(s) 281, 636
Eco31I GGTCTC 2 cut(s) 117, 409
Eco47I GGWCC 4 cut(s) 531, 841, 998, 1088
Eco57I CTGAAG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 726
Eco88I CYCGRG 1 cut(s) 114
EcoT14I CCWWGG 1 cut(s) 1056
EcoT38I GRGCYC 2 cut(s) 281, 636
ErhI CCWWGG 1 cut(s) 1056
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 3 cut(s) 391, 526, 1075
FalI AAGNNNNNCTT 2 cut(s) 608, 640
FaqI GGGAC 2 cut(s) 143, 419
FatI CATG 3 cut(s) 387, 522, 1071
FauI CCCGC 3 cut(s) 67, 160, 197
FbaI TGATCA 1 cut(s) 861
Fnu4HI GCNGC 4 cut(s) 210, 271, 506, 902
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 2 cut(s) 281, 636
Fsp4HI GCNGC 4 cut(s) 210, 271, 506, 902
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 73
GluI GCNGC 4 cut(s) 210, 271, 506, 902
GsaI CCCAGC 2 cut(s) 106, 293
GsuI CTGGAG 1 cut(s) 846
HaeIII GGCC 3 cut(s) 41, 350, 1048
HapII CCGG 6 cut(s) 30, 330, 347, 378, 437, 534
HgaI GACGC 2 cut(s) 322, 581
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 3 cut(s) 391, 526, 1075
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 3 cut(s) 256, 428, 983
HpaII CCGG 6 cut(s) 30, 330, 347, 378, 437, 534
Hpy166II GTNNAC 4 cut(s) 237, 315, 588, 1001
Hpy188I TCNGA 5 cut(s) 125, 309, 568, 736, 879
Hpy188III TCNNGA 5 cut(s) 95, 146, 671, 935, 1113
Hpy8I GTNNAC 4 cut(s) 237, 315, 588, 1001
Hpy99I CGWCG 1 cut(s) 208
HpyAV CCTTC 3 cut(s) 445, 694, 932
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 2 cut(s) 473, 489
HpyCH4V TGCA 3 cut(s) 273, 423, 494
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 59, 270, 276, 975
HpyF3I CTNAG 5 cut(s) 20, 576, 630, 726, 819
HpySE526I ACGT 2 cut(s) 473, 489
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 3 cut(s) 391, 526, 1075
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 861
Kzo9I GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
LmnI GCTCC 2 cut(s) 349, 898
Lsp1109I GCAGC 3 cut(s) 196, 257, 517
LweI GCATC 1 cut(s) 62
MaeI CTAG 1 cut(s) 50
MaeII ACGT 2 cut(s) 473, 489
MaeIII GTNAC 3 cut(s) 13, 595, 1094
MalI GATC 9 cut(s) 127, 144, 284, 299, 640, 684, 863, 921, 1030
MbiI CCGCTC 3 cut(s) 192, 242, 904
MboI GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
MboII GAAGA 2 cut(s) 330, 940
MflI RGATCY 2 cut(s) 125, 919
MhlI GDGCHC 2 cut(s) 281, 636
MluCI AATT 9 cut(s) 169, 182, 556, 642, 744, 810, 854, 886, 991
MlyI GAGTC 2 cut(s) 437, 977
MmeI TCCRAC 2 cut(s) 153, 1089
MseI TTAA 4 cut(s) 216, 372, 615, 717
MslI CAYNNNNRTG 1 cut(s) 656
MspI CCGG 6 cut(s) 30, 330, 347, 378, 437, 534
MspR9I CCNGG 2 cut(s) 437, 535
Mva1269I GAATGC 2 cut(s) 104, 199
MvnI CGCG 6 cut(s) 12, 72, 74, 547, 687, 846
MwoI GCNNNNNNNGC 5 cut(s) 30, 59, 270, 276, 975
NciI CCSGG 2 cut(s) 437, 535
NdeII GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
NlaIII CATG 3 cut(s) 391, 526, 1075
NlaIV GGNNCC 6 cut(s) 127, 345, 756, 900, 921, 999
NmeAIII GCCGAG 3 cut(s) 38, 110, 274
PcsI WCGNNNNNNNCGW 4 cut(s) 153, 200, 330, 678
PctI GAATGC 2 cut(s) 104, 199
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 4 cut(s) 211, 272, 507, 903
Ple19I CGATCG 2 cut(s) 285, 685
PleI GAGTC 2 cut(s) 436, 977
PpsI GAGTC 2 cut(s) 436, 977
PsiI TTATAA 1 cut(s) 783
Psp1406I AACGTT 1 cut(s) 473
PspFI CCCAGC 2 cut(s) 102, 289
PspN4I GGNNCC 6 cut(s) 127, 345, 756, 900, 921, 999
PspPI GGNCC 5 cut(s) 40, 531, 841, 998, 1088
PsuI RGATCY 2 cut(s) 125, 919
PvuI CGATCG 2 cut(s) 285, 685
RgaI GCGATCGC 1 cut(s) 685
RsaI GTAC 2 cut(s) 236, 662
RsaNI GTAC 2 cut(s) 235, 661
RseI CAYNNNNRTG 1 cut(s) 656
SaqAI TTAA 4 cut(s) 216, 372, 615, 717
SatI GCNGC 4 cut(s) 210, 271, 506, 902
Sau3AI GATC 9 cut(s) 125, 142, 282, 297, 638, 682, 861, 919, 1028
Sau96I GGNCC 5 cut(s) 40, 531, 841, 998, 1088
SchI GAGTC 2 cut(s) 437, 977
ScrFI CCNGG 2 cut(s) 437, 535
SduI GDGCHC 2 cut(s) 281, 636
SfaAI GCGATCGC 1 cut(s) 685
SfaNI GCATC 1 cut(s) 62
SgfI GCGATCGC 1 cut(s) 685
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 4 cut(s) 531, 841, 998, 1088
SmiMI CAYNNNNRTG 1 cut(s) 656
Sse9I AATT 9 cut(s) 169, 182, 556, 642, 744, 810, 854, 886, 991
SspMI CTAG 1 cut(s) 50
StyD4I CCNGG 2 cut(s) 435, 533
StyI CCWWGG 1 cut(s) 1056
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 2 cut(s) 476, 492
TaqI TCGA 6 cut(s) 147, 194, 203, 443, 483, 672
TaqII GACCGA 2 cut(s) 99, 373
TasI AATT 9 cut(s) 169, 182, 556, 642, 744, 810, 854, 886, 991
TatI WGTACW 1 cut(s) 234
TauI GCSGC 1 cut(s) 904
TfiI GAWTC 1 cut(s) 256
Tru1I TTAA 4 cut(s) 216, 372, 615, 717
Tru9I TTAA 4 cut(s) 216, 372, 615, 717
TscAI CASTG 3 cut(s) 595, 610, 930
TseI GCWGC 3 cut(s) 209, 270, 505
TspDTI ATGAA 5 cut(s) 464, 696, 790, 897, 927
TspGWI ACGGA 1 cut(s) 421
TspRI CASTG 3 cut(s) 595, 610, 930
VpaK11BI GGWCC 4 cut(s) 531, 841, 998, 1088
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.