MD02G1187600.v1.1

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009896
Physical Location & Seq
Forward (+)
17100872 .. 17101129
258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1187600.v1.1.491

Sequence Viewer

Length: 258 bp
ATGACATTGCAGAAGCCTCGCAGCTTTCCAAGAAAGACATTGGGAGGACCGGTTTTTTTGTCGCGGTTGTTGAATTATTGCTCGGAAGACACTAAGGCTGTGTTCACTGCTGTTACAGTCAGTGTATTGTTTAAGTCTTTCTTGGCTAAGCCCCGATCAATTCCCTCCACTTCAATGTACCCTACTCTCGATGTCGGCGATCGGAGATTGGCGAGATCGCTTTGCGAATTGCGCAGCTCTACTTGGGGCAGTACATGA

Protein Analysis

86

Amino Acids

9.47

Weight (kDa)

10.37

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 31 - 73 2.8e-06 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 233
AccII CGCG 1 cut(s) 64
AciI CCGC 1 cut(s) 64
AfaI GTAC 2 cut(s) 179, 253
AgeI ACCGGT 1 cut(s) 49
AgsI TTSAA 2 cut(s) 73, 174
AluBI AGCT 2 cut(s) 24, 237
AluI AGCT 2 cut(s) 24, 237
ApeKI GCWGC 2 cut(s) 21, 234
AsiGI ACCGGT 1 cut(s) 49
AspLEI GCGC 1 cut(s) 234
AspS9I GGNCC 1 cut(s) 47
AvaII GGWCC 1 cut(s) 47
BbsI GAAGAC 1 cut(s) 93
BbvI GCAGC 2 cut(s) 33, 246
BcgI CGANNNNNNTGC 1 cut(s) 33
BisI GCNGC 2 cut(s) 22, 235
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 2 cut(s) 23, 236
Bme18I GGWCC 1 cut(s) 47
BmgT120I GGNCC 1 cut(s) 47
BpiI GAAGAC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 147
BsaWI WCCGGW 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bse118I RCCGGY 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 5
BseMI GCAATG 1 cut(s) 5
BseXI GCAGC 2 cut(s) 33, 246
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 202
BshTI ACCGGT 1 cut(s) 49
BsiEI CGRYCG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 50
Bsp143I GATC 3 cut(s) 155, 199, 215
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 1 cut(s) 64
BspFNI CGCG 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 5
BsrFI RCCGGY 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 49
BssMI GATC 3 cut(s) 155, 199, 215
Bst4CI ACNGT 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 93, 147
BstFNI CGCG 1 cut(s) 64
BstHHI GCGC 1 cut(s) 234
BstKTI GATC 3 cut(s) 158, 202, 218
BstMBI GATC 3 cut(s) 155, 199, 215
BstMCI CGRYCG 1 cut(s) 202
BstMWI GCNNNNNNNGC 1 cut(s) 231
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 2 cut(s) 33, 246
BstV2I GAAGAC 1 cut(s) 93
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 127
CfoI GCGC 1 cut(s) 234
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 47
Csp6I GTAC 2 cut(s) 178, 252
CspAI ACCGGT 1 cut(s) 49
CviAII CATG 1 cut(s) 255
CviJI RGCY 6 cut(s) 16, 24, 98, 146, 151, 237
CviKI_1 RGCY 6 cut(s) 16, 24, 98, 146, 151, 237
CviQI GTAC 2 cut(s) 178, 252
DdeI CTNAG 2 cut(s) 93, 147
DpnI GATC 3 cut(s) 157, 201, 217
DpnII GATC 3 cut(s) 155, 199, 215
Eco47I GGWCC 1 cut(s) 47
FaeI CATG 1 cut(s) 258
FaiI YATR 1 cut(s) 256
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FatI CATG 1 cut(s) 254
Fnu4HI GCNGC 2 cut(s) 22, 235
Fsp4HI GCNGC 2 cut(s) 22, 235
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 1 cut(s) 233
GluI GCNGC 2 cut(s) 22, 235
HapII CCGG 1 cut(s) 50
HhaI GCGC 1 cut(s) 234
Hin1II CATG 1 cut(s) 258
Hin6I GCGC 1 cut(s) 232
HinP1I GCGC 1 cut(s) 232
HpaII CCGG 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 105
Hpy188I TCNGA 2 cut(s) 85, 204
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 1 cut(s) 105
HpyCH4III ACNGT 1 cut(s) 118
HpyCH4V TGCA 1 cut(s) 10
HpyF10VI GCNNNNNNNGC 1 cut(s) 231
HpyF3I CTNAG 2 cut(s) 93, 147
Hsp92II CATG 1 cut(s) 258
HspAI GCGC 1 cut(s) 232
Kzo9I GATC 3 cut(s) 155, 199, 215
LpnPI CCDG 1 cut(s) 63
Lsp1109I GCAGC 2 cut(s) 33, 246
MaeIII GTNAC 1 cut(s) 112
MalI GATC 3 cut(s) 157, 201, 217
MboI GATC 3 cut(s) 155, 199, 215
MboII GAAGA 1 cut(s) 98
MluCI AATT 3 cut(s) 73, 159, 227
MnlI CCTC 3 cut(s) 27, 38, 175
MseI TTAA 1 cut(s) 132
MslI CAYNNNNRTG 1 cut(s) 173
MspI CCGG 1 cut(s) 50
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 231
NdeII GATC 3 cut(s) 155, 199, 215
NlaIII CATG 1 cut(s) 258
NsbI TGCGCA 1 cut(s) 233
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PinAI ACCGGT 1 cut(s) 49
PkrI GCNGC 2 cut(s) 23, 236
Ple19I CGATCG 1 cut(s) 202
PspPI GGNCC 1 cut(s) 47
PvuI CGATCG 1 cut(s) 202
RsaI GTAC 2 cut(s) 179, 253
RsaNI GTAC 2 cut(s) 178, 252
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 1 cut(s) 132
SatI GCNGC 2 cut(s) 22, 235
Sau3AI GATC 3 cut(s) 155, 199, 215
Sau96I GGNCC 1 cut(s) 47
SetI ASST 2 cut(s) 26, 239
SgeI CNNG 9 cut(s) 30, 42, 62, 75, 94, 154, 165, 200, 225
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 3 cut(s) 73, 159, 227
SsiI CCGC 1 cut(s) 64
TaaI ACNGT 1 cut(s) 118
TaqI TCGA 1 cut(s) 189
TasI AATT 3 cut(s) 73, 159, 227
TatI WGTACW 1 cut(s) 251
Tru1I TTAA 1 cut(s) 132
Tru9I TTAA 1 cut(s) 132
TscAI CASTG 2 cut(s) 112, 127
TseI GCWGC 2 cut(s) 21, 234
TspRI CASTG 2 cut(s) 112, 127
VpaK11BI GGWCC 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.