Rh6BG413000

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009896
Physical Location & Seq
Reverse (-)
63811028 .. 63813747
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG413000.1

Sequence Viewer

Length: 750 bp
ATGGCGATCCGCGTCACCCTAGGGTTTTCCGGCTACGTAGCCCAAAACCTAGCCTCCTCCGCCAGTATTCGCGCCAGTAATTGCCGCGGCGTCCACGAATGCTGGGTTAGGTCTCTGCTGTTCGGCACCAACCAGAAGGCCGATCTCGACCCCTCCGGCACCGTCCCCAGTTACCGGTCCAAATTCGCCGCCAGGTCCAAGGCTCCGACCTCCCTCTACGGCGCCCTTGCCGAAGAATTCCTCTGCCAAAGCTCTAAGACCCCGATTATGTCCACCTTGATCTCCGTTTTGAAGTCAACGGCCGCCGTCACCGGTCCTTCTCTGGCTTCCCCGTCTCTTGGCCTCGGAATCTCGCCGGTCAAACCCGGTTCGATCATGCCGTTCTTGCAGGCCTCCAAGTGGCTTCCCTGCAACGAATCTGTTCCGGCGAAATTGGTGAGCGAGATGGACAGAAAACTGCGTTTGGATGATGATGAGGTGGTGGTCACTGAGGTGGTGGCCTCTGCCTCGGAGTTGAGTAAGAATGGATTGCAGAGGAGCGGTTGGTTATCGCGCCTCTTGAGTTCTTGCTCCGAAGACGCAAAGGCAGTCTTCACTGCTGTGACTGTCAGCGTTCTGTTCCGCTCATCCTTGGCCGAGCCCAGGTCCATTCCTTCCACCTCTATGTACCCTACTCTCGATGTGGGTGATCGTGTTCTAGCTGAGAAGGTCAGTAATGTTCTCATCATTAATCTCGAATTATTGGTGTGA

Protein Analysis

249

Amino Acids

26.51

Weight (kDa)

8.83

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 194 - 238 8.3e-07 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 125, 158, 221
AccBSI CCGCTC 2 cut(s) 540, 624
AccII CGCG 4 cut(s) 12, 72, 87, 553
AciI CCGC 8 cut(s) 10, 60, 85, 87, 189, 303, 540, 622
AcoI YGGCCR 2 cut(s) 300, 633
AcsI RAATTY 2 cut(s) 182, 236
AcyI GRCGYC 2 cut(s) 90, 222
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 4 cut(s) 174, 338, 399, 642
AgeI ACCGGT 2 cut(s) 174, 311
AgsI TTSAA 1 cut(s) 292
AjnI CCWGG 2 cut(s) 191, 641
AleI CACNNNNGTG 2 cut(s) 491, 599
AluBI AGCT 2 cut(s) 252, 701
AluI AGCT 2 cut(s) 252, 701
Alw26I GTCTC 2 cut(s) 117, 339
AoxI GGCC 6 cut(s) 138, 300, 340, 390, 498, 633
ApoI RAATTY 2 cut(s) 182, 236
AseI ATTAAT 1 cut(s) 729
AsiGI ACCGGT 2 cut(s) 174, 311
Asp700I GAANNNNTTC 1 cut(s) 420
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 3 cut(s) 74, 224, 555
AspS9I GGNCC 4 cut(s) 177, 195, 314, 645
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 4 cut(s) 7, 301, 448, 698
AvaII GGWCC 4 cut(s) 177, 195, 314, 645
AvrII CCTAGG 1 cut(s) 19
BanI GGYRCC 3 cut(s) 125, 158, 221
BanII GRGCYC 1 cut(s) 642
BbsI GAAGAC 2 cut(s) 582, 583
BccI CCATC 1 cut(s) 439
BceAI ACGGC 4 cut(s) 235, 290, 315, 364
BciT130I CCWGG 2 cut(s) 193, 643
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 2 cut(s) 117, 339
BfaI CTAG 3 cut(s) 20, 50, 698
BfoI RGCGCY 1 cut(s) 225
BisI GCNGC 4 cut(s) 85, 88, 189, 303
BlnI CCTAGG 1 cut(s) 19
BlsI GCNGC 4 cut(s) 86, 89, 190, 304
Bme1390I CCNGG 3 cut(s) 193, 366, 643
Bme18I GGWCC 4 cut(s) 177, 195, 314, 645
BmgT120I GGNCC 4 cut(s) 177, 195, 314, 645
BmiI GGNNCC 4 cut(s) 127, 160, 204, 223
BmrFI CCNGG 3 cut(s) 193, 366, 643
BmrI ACTGGG 1 cut(s) 162
BmuI ACTGGG 1 cut(s) 162
BpiI GAAGAC 2 cut(s) 582, 583
BpuEI CTTGAG 1 cut(s) 580
BpuMI CCSGG 1 cut(s) 366
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 2 cut(s) 90, 222
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 7 cut(s) 19, 85, 198, 343, 507, 630, 641
BsaWI WCCGGW 2 cut(s) 174, 311
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 4 cut(s) 174, 338, 399, 642
Bse118I RCCGGY 3 cut(s) 174, 311, 355
Bse1I ACTGG 3 cut(s) 63, 75, 168
BseBI CCWGG 2 cut(s) 193, 643
BseDI CCNNGG 7 cut(s) 19, 85, 198, 343, 507, 630, 641
BseGI GGATG 2 cut(s) 472, 626
BseLI CCNNNNNNNGG 4 cut(s) 174, 338, 399, 642
BseMII CTCAG 2 cut(s) 480, 693
BseNI ACTGG 3 cut(s) 63, 75, 168
BseRI GAGGAG 2 cut(s) 46, 550
BseX3I CGGCCG 1 cut(s) 300
BseYI CCCAGC 1 cut(s) 102
Bsh1236I CGCG 4 cut(s) 12, 72, 87, 553
Bsh1285I CGRYCG 1 cut(s) 303
BshFI GGCC 6 cut(s) 140, 302, 342, 392, 500, 635
BshNI GGYRCC 3 cut(s) 125, 158, 221
BshTI ACCGGT 2 cut(s) 174, 311
BsiEI CGRYCG 1 cut(s) 303
BsiSI CCGG 7 cut(s) 30, 156, 175, 312, 356, 366, 425
BslFI GGGAC 1 cut(s) 149
BslI CCNNNNNNNGG 4 cut(s) 174, 338, 399, 642
BsmAI GTCTC 2 cut(s) 117, 339
BsmBI CGTCTC 1 cut(s) 339
BsmFI GGGAC 1 cut(s) 149
BsmI GAATGC 1 cut(s) 104
BsnI GGCC 6 cut(s) 140, 302, 342, 392, 500, 635
Bso31I GGTCTC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 642
Bsp143I GATC 5 cut(s) 6, 142, 279, 372, 688
BspACI CCGC 8 cut(s) 10, 60, 85, 87, 189, 303, 540, 622
BspANI GGCC 6 cut(s) 140, 302, 342, 392, 500, 635
BspCNI CTCAG 2 cut(s) 481, 694
BspFNI CGCG 4 cut(s) 12, 72, 87, 553
BspLI GGNNCC 4 cut(s) 127, 160, 204, 223
BspT107I GGYRCC 3 cut(s) 125, 158, 221
BspTNI GGTCTC 1 cut(s) 117
BsrBI CCGCTC 2 cut(s) 540, 624
BsrFI RCCGGY 3 cut(s) 174, 311, 355
BsrI ACTGG 3 cut(s) 63, 75, 168
BssAI RCCGGY 3 cut(s) 174, 311, 355
BssECI CCNNGG 7 cut(s) 19, 85, 198, 343, 507, 630, 641
BssMI GATC 5 cut(s) 6, 142, 279, 372, 688
BssNI GRCGYC 2 cut(s) 90, 222
BssT1I CCWWGG 3 cut(s) 19, 198, 630
Bst2UI CCWGG 2 cut(s) 193, 643
Bst4CI ACNGT 2 cut(s) 163, 607
BstACI GRCGYC 2 cut(s) 90, 222
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 390
BstDEI CTNAG 3 cut(s) 255, 489, 702
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 2 cut(s) 472, 626
BstFNI CGCG 4 cut(s) 12, 72, 87, 553
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 74, 224, 555
BstKTI GATC 5 cut(s) 9, 145, 282, 375, 691
BstMAI GTCTC 2 cut(s) 117, 339
BstMBI GATC 5 cut(s) 6, 142, 279, 372, 688
BstMCI CGRYCG 1 cut(s) 303
BstMWI GCNNNNNNNGC 2 cut(s) 59, 385
BstNI CCWGG 2 cut(s) 193, 643
BstSCI CCNGG 3 cut(s) 191, 364, 641
BstSNI TACGTA 1 cut(s) 37
BstUI CGCG 4 cut(s) 12, 72, 87, 553
BstV2I GAAGAC 2 cut(s) 582, 583
BstZI CGGCCG 1 cut(s) 300
BsuRI GGCC 6 cut(s) 140, 302, 342, 392, 500, 635
BtgI CCRYGG 1 cut(s) 85
BtsCI GGATG 2 cut(s) 472, 626
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 2 cut(s) 486, 594
Cac8I GCNNGC 1 cut(s) 390
CfoI GCGC 3 cut(s) 74, 224, 555
Cfr10I RCCGGY 3 cut(s) 174, 311, 355
Cfr13I GGNCC 4 cut(s) 177, 195, 314, 645
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 2 cut(s) 79, 587
Csp6I GTAC 1 cut(s) 667
CspAI ACCGGT 2 cut(s) 174, 311
CviAII CATG 1 cut(s) 376
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 3 cut(s) 255, 489, 702
DinI GGCGCC 1 cut(s) 223
DpnI GATC 5 cut(s) 8, 144, 281, 374, 690
DpnII GATC 5 cut(s) 6, 142, 279, 372, 688
EaeI YGGCCR 2 cut(s) 300, 633
EagI CGGCCG 1 cut(s) 300
EciI GGCGGA 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 300
Eco105I TACGTA 1 cut(s) 37
Eco130I CCWWGG 3 cut(s) 19, 198, 630
Eco147I AGGCCT 1 cut(s) 392
Eco24I GRGCYC 1 cut(s) 642
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 4 cut(s) 177, 195, 314, 645
Eco52I CGGCCG 1 cut(s) 300
EcoRI GAATTC 1 cut(s) 236
EcoRII CCWGG 2 cut(s) 191, 641
EcoT14I CCWWGG 3 cut(s) 19, 198, 630
EcoT38I GRGCYC 1 cut(s) 642
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 3 cut(s) 19, 198, 630
Esp3I CGTCTC 1 cut(s) 339
FaeI CATG 1 cut(s) 379
FaiI YATR 3 cut(s) 269, 377, 665
FalI AAGNNNNNCTT 2 cut(s) 575, 607
FaqI GGGAC 1 cut(s) 149
FatI CATG 1 cut(s) 375
Fnu4HI GCNGC 4 cut(s) 85, 88, 189, 303
FokI GGATG 2 cut(s) 479, 613
FriOI GRGCYC 1 cut(s) 642
Fsp4HI GCNGC 4 cut(s) 85, 88, 189, 303
FspBI CTAG 3 cut(s) 20, 50, 698
GlaI GCGC 3 cut(s) 73, 223, 554
GluI GCNGC 4 cut(s) 85, 88, 189, 303
GsaI CCCAGC 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 6 cut(s) 140, 302, 342, 392, 500, 635
HapII CCGG 7 cut(s) 30, 156, 175, 312, 356, 366, 425
HgaI GACGC 2 cut(s) 79, 587
HhaI GCGC 3 cut(s) 74, 224, 555
Hin1I GRCGYC 2 cut(s) 90, 222
Hin1II CATG 1 cut(s) 379
Hin6I GCGC 3 cut(s) 72, 222, 553
HinP1I GCGC 3 cut(s) 72, 222, 553
HincII GTYRAC 1 cut(s) 297
HindII GTYRAC 1 cut(s) 297
HinfI GANTC 2 cut(s) 348, 416
HpaII CCGG 7 cut(s) 30, 156, 175, 312, 356, 366, 425
HphI GGTGA 4 cut(s) 7, 301, 448, 698
Hpy166II GTNNAC 3 cut(s) 94, 273, 297
Hpy188I TCNGA 4 cut(s) 207, 347, 511, 574
Hpy188III TCNNGA 4 cut(s) 146, 559, 677, 734
Hpy8I GTNNAC 3 cut(s) 94, 273, 297
HpyAV CCTTC 4 cut(s) 130, 327, 663, 700
HpyCH4III ACNGT 2 cut(s) 163, 607
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 3 cut(s) 388, 411, 532
HpyF10VI GCNNNNNNNGC 2 cut(s) 59, 385
HpyF3I CTNAG 3 cut(s) 255, 489, 702
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 2 cut(s) 90, 222
Hsp92II CATG 1 cut(s) 379
HspAI GCGC 3 cut(s) 72, 222, 553
KasI GGCGCC 1 cut(s) 221
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 5 cut(s) 6, 142, 279, 372, 688
LmnI GCTCC 3 cut(s) 208, 537, 575
MaeI CTAG 3 cut(s) 20, 50, 698
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 5 cut(s) 13, 170, 307, 484, 601
MalI GATC 5 cut(s) 8, 144, 281, 374, 690
MbiI CCGCTC 2 cut(s) 540, 624
MboI GATC 5 cut(s) 6, 142, 279, 372, 688
MboII GAAGA 3 cut(s) 245, 583, 587
MhlI GDGCHC 1 cut(s) 642
MluCI AATT 5 cut(s) 79, 182, 236, 431, 737
Mly113I GGCGCC 1 cut(s) 222
MmeI TCCRAC 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 1 cut(s) 729
MslI CAYNNNNRTG 3 cut(s) 491, 599, 662
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 7 cut(s) 30, 156, 175, 312, 356, 366, 425
MspR9I CCNGG 3 cut(s) 193, 366, 643
Mva1269I GAATGC 1 cut(s) 104
MvaI CCWGG 2 cut(s) 193, 643
MvnI CGCG 4 cut(s) 12, 72, 87, 553
MwoI GCNNNNNNNGC 2 cut(s) 59, 385
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 1 cut(s) 366
NdeII GATC 5 cut(s) 6, 142, 279, 372, 688
NlaIII CATG 1 cut(s) 379
NlaIV GGNNCC 4 cut(s) 127, 160, 204, 223
NmeAIII GCCGAG 1 cut(s) 661
NmuCI GTSAC 4 cut(s) 13, 307, 484, 601
OliI CACNNNNGTG 2 cut(s) 491, 599
PceI AGGCCT 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 348, 416
PinAI ACCGGT 2 cut(s) 174, 311
PkrI GCNGC 4 cut(s) 86, 89, 190, 304
PluTI GGCGCC 1 cut(s) 225
Ppu21I YACGTR 1 cut(s) 37
PshBI ATTAAT 1 cut(s) 729
Psp6I CCWGG 2 cut(s) 191, 641
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 2 cut(s) 191, 641
PspN4I GGNNCC 4 cut(s) 127, 160, 204, 223
PspPI GGNCC 4 cut(s) 177, 195, 314, 645
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
RseI CAYNNNNRTG 3 cut(s) 491, 599, 662
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 4 cut(s) 85, 88, 189, 303
Sau3AI GATC 5 cut(s) 6, 142, 279, 372, 688
Sau96I GGNCC 4 cut(s) 177, 195, 314, 645
ScrFI CCNGG 3 cut(s) 193, 366, 643
SduI GDGCHC 1 cut(s) 642
SfoI GGCGCC 1 cut(s) 223
Sfr303I CCGCGG 1 cut(s) 88
SgrBI CCGCGG 1 cut(s) 88
SinI GGWCC 4 cut(s) 177, 195, 314, 645
SmiMI CAYNNNNRTG 3 cut(s) 491, 599, 662
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
SnaBI TACGTA 1 cut(s) 37
Sse9I AATT 5 cut(s) 79, 182, 236, 431, 737
SseBI AGGCCT 1 cut(s) 392
SsiI CCGC 8 cut(s) 10, 60, 85, 87, 189, 303, 540, 622
SspDI GGCGCC 1 cut(s) 221
SspMI CTAG 3 cut(s) 20, 50, 698
StuI AGGCCT 1 cut(s) 392
StyD4I CCNGG 3 cut(s) 191, 364, 641
StyI CCWWGG 3 cut(s) 19, 198, 630
TaaI ACNGT 2 cut(s) 163, 607
TaiI ACGT 1 cut(s) 39
TaqI TCGA 4 cut(s) 147, 371, 678, 735
TasI AATT 5 cut(s) 79, 182, 236, 431, 737
TauI GCSGC 4 cut(s) 87, 90, 191, 305
TfiI GAWTC 2 cut(s) 348, 416
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 2 cut(s) 493, 601
TseFI GTSAC 4 cut(s) 13, 307, 484, 601
Tsp45I GTSAC 4 cut(s) 13, 307, 484, 601
TspGWI ACGGA 1 cut(s) 274
TspRI CASTG 2 cut(s) 493, 601
VpaK11BI GGWCC 4 cut(s) 177, 195, 314, 645
VspI ATTAAT 1 cut(s) 729
XapI RAATTY 2 cut(s) 182, 236
XmaJI CCTAGG 1 cut(s) 19
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 3 cut(s) 20, 50, 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.