FvH4_2g40680

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009896
Physical Location & Seq
Forward (+)
28947372 .. 28949966
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40680.t1

Sequence Viewer

Length: 1122 bp
ATGGCGATCCGCGTCACCCTCAGTTTTTCCGGCTACGTGGCCCAAAACCTAGCCTCCTCCGCCAGTATCCGCGTCACCAATTGCCGCGGAGTCCACGAATGCTGGGTTAGGTCTCTTCTCTTCGGCACCAACCAGAAGGCCGATCTCGACCCCTCCGGCTCCGTCCCCAATTACCAATCCAAATTCAGACCTAAGGCTGCTCCGGCCTCCCTCTACAGCGCCCTCGCCGAAGAGTTTCTCGGCAAGAAGGCCAACACTCCTATTATGTCCACTCTGATCTCCGTTTTGAAGTCAACGGCCGCCGTCACCGGTTCGTCTTTGGCGTCTCCGTCTCTTGTTAACGGAATTTCGCCTGTCAAGCAAGGGACGATCATGCCGTTCATGCAGGCGTCCAAGTGGCTTCCCTGCAACGAGTCTGTTCCGGCCAAATTGGTGAGCGAGATGGACAAGAAAGTTGTGCGTGGGGATGATGAGGTGTTGGTCTCTGAGGCGGCGGCCTCTGCTGCTGCGTCGGAGTTGAGCAAGAGTGGATTGCAGAGGAGCGGCTGGTTATCGCGCCTCTTGAATTCTTGCTCTGATGATGCTAAGGCAGTGTTTACTGCTGTCACCGTCAGCGTTTTGTTCCGGTCTTCCTTGGCCGAGCCGAGGTCCATTCCTTCCTCCTCTATGTATCCTACTCTGGATGTAGGTGATCGTGTTTTGGCTGAGAAGGTTTCATATTTGTTCAGAAAGCCTGAGGTTTCAGATATTGTAATTTTCAAGGCACCTCCAATCTTGCAGGAAATTGGCTTTAGCTCAGGCGATGTATTTATAAAAAGAATTGTGGCCAAGGCTGGAGATTATGTAGAGGTACATGATGGGAAATTGTTGGTGAACGGTGTAATTCAAAATGAAAATTACATATTGGAGCCTCTTGCCTATGAAATGGAACAAATTCTCATTCCAGAAGGCTATGTCTTTGTGATGGGGGATAACCGCAACAACAGTTTTGACTCTCATAACTGGGGTCCCCTTCCTGTAAAAAACATTCTTGGCAGATCCGTGTTTCGCTACTGGCCTCCCTCCAAAGTTTCTGACATTATGCATGAACCACAGGTAGAAAAATCTGTGGCAATTTCCTGA

Protein Analysis

374

Amino Acids

40.48

Weight (kDa)

8.22

Isoelectric Point (pI)

45.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 195 - 352 6.5e-43 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
AccB1I GGYRCC 2 cut(s) 125, 763
AccBSI CCGCTC 1 cut(s) 543
AccII CGCG 4 cut(s) 12, 72, 87, 556
AclWI GGATC 1 cut(s) 1032
AcoI YGGCCR 4 cut(s) 297, 423, 636, 825
AcsI RAATTY 4 cut(s) 182, 345, 565, 933
AcyI GRCGYC 2 cut(s) 323, 389
AfaI GTAC 1 cut(s) 852
AfiI CCNNNNNNNGG 1 cut(s) 645
AgeI ACCGGT 1 cut(s) 308
AgsI TTSAA 4 cut(s) 289, 565, 760, 887
AluBI AGCT 1 cut(s) 795
AluI AGCT 1 cut(s) 795
Alw26I GTCTC 4 cut(s) 117, 330, 336, 487
AlwI GGATC 1 cut(s) 1032
ApeKI GCWGC 3 cut(s) 197, 503, 506
ApoI RAATTY 4 cut(s) 182, 345, 565, 933
AsiGI ACCGGT 1 cut(s) 308
Asp700I GAANNNNTTC 2 cut(s) 234, 933
AspLEI GCGC 2 cut(s) 221, 558
AspS9I GGNCC 3 cut(s) 40, 648, 1007
AsuHPI GGTGA 7 cut(s) 7, 67, 298, 445, 598, 701, 883
AvaII GGWCC 2 cut(s) 648, 1007
AxyI CCTNAGG 2 cut(s) 192, 735
BalI TGGCCA 1 cut(s) 827
BanI GGYRCC 2 cut(s) 125, 763
BbsI GAAGAC 1 cut(s) 621
BbvI GCAGC 3 cut(s) 184, 490, 493
BccI CCATC 3 cut(s) 436, 851, 958
BceAI ACGGC 3 cut(s) 287, 312, 361
BciVI GTATCC 2 cut(s) 77, 681
BcoDI GTCTC 4 cut(s) 117, 330, 336, 487
BfaI CTAG 1 cut(s) 50
BfmI CTRYAG 1 cut(s) 214
BfoI RGCGCY 1 cut(s) 222
BfuI GTATCC 2 cut(s) 77, 681
BisI GCNGC 8 cut(s) 85, 198, 300, 492, 495, 504, 507, 544
BlsI GCNGC 8 cut(s) 86, 199, 301, 493, 496, 505, 508, 545
Bme18I GGWCC 2 cut(s) 648, 1007
BmgT120I GGNCC 3 cut(s) 40, 648, 1007
BmiI GGNNCC 6 cut(s) 127, 160, 765, 909, 1008, 1009
BmrI ACTGGG 1 cut(s) 1012
BmsI GCATC 1 cut(s) 571
BmuI ACTGGG 1 cut(s) 1012
BpiI GAAGAC 1 cut(s) 621
BpmI CTGGAG 1 cut(s) 855
Bpu10I CCTNAGC 2 cut(s) 585, 796
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 2 cut(s) 323, 389
BsaI GGTCTC 2 cut(s) 117, 487
BsaJI CCNNGG 4 cut(s) 85, 633, 644, 828
BsaWI WCCGGW 2 cut(s) 308, 624
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 828, 858
Bsc4I CCNNNNNNNGG 1 cut(s) 645
Bse118I RCCGGY 1 cut(s) 308
Bse1I ACTGG 3 cut(s) 63, 1007, 1058
Bse21I CCTNAGG 2 cut(s) 192, 735
BseDI CCNNGG 4 cut(s) 85, 633, 644, 828
BseGI GGATG 2 cut(s) 472, 688
BseLI CCNNNNNNNGG 1 cut(s) 645
BseMII CTCAG 5 cut(s) 34, 477, 696, 726, 810
BseNI ACTGG 3 cut(s) 63, 1007, 1058
BseRI GAGGAG 3 cut(s) 46, 553, 652
BseX3I CGGCCG 1 cut(s) 297
BseXI GCAGC 3 cut(s) 184, 490, 493
BseYI CCCAGC 1 cut(s) 102
Bsh1236I CGCG 4 cut(s) 12, 72, 87, 556
Bsh1285I CGRYCG 1 cut(s) 300
BshNI GGYRCC 2 cut(s) 125, 763
BshTI ACCGGT 1 cut(s) 308
BsiEI CGRYCG 1 cut(s) 300
BsiSI CCGG 6 cut(s) 30, 156, 203, 309, 422, 625
BslFI GGGAC 3 cut(s) 149, 379, 993
BslI CCNNNNNNNGG 1 cut(s) 645
BsmAI GTCTC 4 cut(s) 117, 330, 336, 487
BsmBI CGTCTC 2 cut(s) 330, 336
BsmFI GGGAC 3 cut(s) 149, 379, 993
BsmI GAATGC 1 cut(s) 104
Bso31I GGTCTC 2 cut(s) 117, 487
Bsp143I GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
BspCNI CTCAG 5 cut(s) 33, 478, 697, 727, 809
BspFNI CGCG 4 cut(s) 12, 72, 87, 556
BspLI GGNNCC 6 cut(s) 127, 160, 765, 909, 1008, 1009
BspPI GGATC 1 cut(s) 1032
BspT107I GGYRCC 2 cut(s) 125, 763
BspTNI GGTCTC 2 cut(s) 117, 487
BsrBI CCGCTC 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 308
BsrI ACTGG 3 cut(s) 63, 1007, 1058
BssAI RCCGGY 1 cut(s) 308
BssECI CCNNGG 4 cut(s) 85, 633, 644, 828
BssMI GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
BssNI GRCGYC 2 cut(s) 323, 389
BssT1I CCWWGG 2 cut(s) 633, 828
Bst4CI ACNGT 3 cut(s) 610, 878, 986
Bst6I CTCTTC 3 cut(s) 120, 125, 225
BstACI GRCGYC 2 cut(s) 323, 389
BstBAI YACGTR 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 7 cut(s) 20, 192, 486, 585, 705, 735, 796
BstDSI CCRYGG 1 cut(s) 85
BstF5I GGATG 2 cut(s) 472, 688
BstFNI CGCG 4 cut(s) 12, 72, 87, 556
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 558
BstKTI GATC 6 cut(s) 9, 145, 279, 372, 694, 1040
BstMAI GTCTC 4 cut(s) 117, 330, 336, 487
BstMBI GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
BstMCI CGRYCG 1 cut(s) 300
BstMWI GCNNNNNNNGC 6 cut(s) 59, 203, 358, 382, 500, 503
BstSFI CTRYAG 1 cut(s) 214
BstUI CGCG 4 cut(s) 12, 72, 87, 556
BstV1I GCAGC 3 cut(s) 184, 490, 493
BstV2I GAAGAC 1 cut(s) 621
BstX2I RGATCY 1 cut(s) 1037
BstYI RGATCY 1 cut(s) 1037
BstZI CGGCCG 1 cut(s) 297
Bsu36I CCTNAGG 2 cut(s) 192, 735
BsuI GTATCC 2 cut(s) 77, 681
BtgI CCRYGG 1 cut(s) 85
BtgZI GCGATG 1 cut(s) 816
BtsCI GGATG 2 cut(s) 472, 688
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 1 cut(s) 597
Cac8I GCNNGC 1 cut(s) 387
CfoI GCGC 2 cut(s) 221, 558
Cfr10I RCCGGY 1 cut(s) 308
Cfr13I GGNCC 3 cut(s) 40, 648, 1007
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 4 cut(s) 61, 312, 378, 498
Csp6I GTAC 1 cut(s) 851
CspAI ACCGGT 1 cut(s) 308
CviAII CATG 4 cut(s) 373, 382, 854, 1085
CviQI GTAC 1 cut(s) 851
DdeI CTNAG 7 cut(s) 20, 192, 486, 585, 705, 735, 796
DpnI GATC 6 cut(s) 8, 144, 278, 371, 693, 1039
DpnII GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
EaeI YGGCCR 4 cut(s) 297, 423, 636, 825
EagI CGGCCG 1 cut(s) 297
Eam1104I CTCTTC 3 cut(s) 120, 125, 225
EarI CTCTTC 3 cut(s) 120, 125, 225
EciI GGCGGA 1 cut(s) 49
EclXI CGGCCG 1 cut(s) 297
Eco130I CCWWGG 2 cut(s) 633, 828
Eco31I GGTCTC 2 cut(s) 117, 487
Eco47I GGWCC 2 cut(s) 648, 1007
Eco52I CGGCCG 1 cut(s) 297
Eco81I CCTNAGG 2 cut(s) 192, 735
EcoO109I RGGNCCY 1 cut(s) 1007
EcoRI GAATTC 1 cut(s) 565
EcoT14I CCWWGG 2 cut(s) 633, 828
EcoT22I ATGCAT 1 cut(s) 1086
ErhI CCWWGG 2 cut(s) 633, 828
Esp3I CGTCTC 2 cut(s) 330, 336
FaeI CATG 4 cut(s) 376, 385, 857, 1088
FaqI GGGAC 3 cut(s) 149, 379, 993
FatI CATG 4 cut(s) 372, 381, 853, 1084
Fnu4HI GCNGC 8 cut(s) 85, 198, 300, 492, 495, 504, 507, 544
FokI GGATG 2 cut(s) 479, 695
Fsp4HI GCNGC 8 cut(s) 85, 198, 300, 492, 495, 504, 507, 544
FspBI CTAG 1 cut(s) 50
GlaI GCGC 2 cut(s) 220, 557
GluI GCNGC 8 cut(s) 85, 198, 300, 492, 495, 504, 507, 544
GsaI CCCAGC 1 cut(s) 106
GsuI CTGGAG 1 cut(s) 855
HaeII RGCGCY 1 cut(s) 222
HapII CCGG 6 cut(s) 30, 156, 203, 309, 422, 625
HgaI GACGC 4 cut(s) 61, 312, 378, 498
HhaI GCGC 2 cut(s) 221, 558
Hin1I GRCGYC 2 cut(s) 323, 389
Hin1II CATG 4 cut(s) 376, 385, 857, 1088
Hin6I GCGC 2 cut(s) 219, 556
HinP1I GCGC 2 cut(s) 219, 556
HincII GTYRAC 2 cut(s) 294, 340
HindII GTYRAC 2 cut(s) 294, 340
HinfI GANTC 3 cut(s) 90, 413, 992
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 6 cut(s) 30, 156, 203, 309, 422, 625
HphI GGTGA 7 cut(s) 7, 67, 298, 445, 598, 701, 883
Hpy166II GTNNAC 6 cut(s) 94, 270, 294, 340, 597, 874
Hpy188I TCNGA 8 cut(s) 188, 276, 487, 514, 577, 728, 745, 1075
Hpy188III TCNNGA 5 cut(s) 146, 562, 680, 944, 1119
Hpy8I GTNNAC 6 cut(s) 94, 270, 294, 340, 597, 874
Hpy99I CGWCG 1 cut(s) 514
HpyAV CCTTC 6 cut(s) 130, 241, 666, 703, 941, 1022
HpyCH4III ACNGT 3 cut(s) 610, 878, 986
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 5 cut(s) 385, 408, 535, 778, 1084
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 203, 358, 382, 500, 503
HpyF3I CTNAG 7 cut(s) 20, 192, 486, 585, 705, 735, 796
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 2 cut(s) 323, 389
Hsp92II CATG 4 cut(s) 376, 385, 857, 1088
HspAI GCGC 2 cut(s) 219, 556
KflI GGGWCCC 1 cut(s) 1007
KspAI GTTAAC 1 cut(s) 340
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
LmnI GCTCC 4 cut(s) 164, 205, 540, 907
Lsp1109I GCAGC 3 cut(s) 184, 490, 493
LweI GCATC 1 cut(s) 571
MaeI CTAG 1 cut(s) 50
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 4 cut(s) 13, 73, 304, 604
MalI GATC 6 cut(s) 8, 144, 278, 371, 693, 1039
MbiI CCGCTC 1 cut(s) 543
MboI GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
MboII GAAGA 4 cut(s) 107, 112, 242, 621
MfeI CAATTG 1 cut(s) 79
MflI RGATCY 1 cut(s) 1037
MlsI TGGCCA 1 cut(s) 827
MluNI TGGCCA 1 cut(s) 827
MlyI GAGTC 3 cut(s) 99, 422, 986
MmeI TCCRAC 1 cut(s) 492
Mox20I TGGCCA 1 cut(s) 827
Mph1103I ATGCAT 1 cut(s) 1086
MroXI GAANNNNTTC 2 cut(s) 234, 933
MscI TGGCCA 1 cut(s) 827
MseI TTAA 1 cut(s) 339
Msp20I TGGCCA 1 cut(s) 827
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 6 cut(s) 30, 156, 203, 309, 422, 625
MunI CAATTG 1 cut(s) 79
Mva1269I GAATGC 1 cut(s) 104
MvnI CGCG 4 cut(s) 12, 72, 87, 556
MwoI GCNNNNNNNGC 6 cut(s) 59, 203, 358, 382, 500, 503
NdeII GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
NlaIII CATG 4 cut(s) 376, 385, 857, 1088
NlaIV GGNNCC 6 cut(s) 127, 160, 765, 909, 1008, 1009
NmeAIII GCCGAG 3 cut(s) 219, 664, 669
NmuCI GTSAC 4 cut(s) 13, 73, 304, 604
NsiI ATGCAT 1 cut(s) 1086
PcsI WCGNNNNNNNCGW 2 cut(s) 320, 374
PctI GAATGC 1 cut(s) 104
PdmI GAANNNNTTC 2 cut(s) 234, 933
PinAI ACCGGT 1 cut(s) 308
PkrI GCNGC 8 cut(s) 86, 199, 301, 493, 496, 505, 508, 545
PleI GAGTC 3 cut(s) 98, 421, 986
PpsI GAGTC 3 cut(s) 98, 421, 986
Ppu21I YACGTR 1 cut(s) 37
PpuMI RGGWCCY 1 cut(s) 1007
PsiI TTATAA 1 cut(s) 812
Psp5II RGGWCCY 1 cut(s) 1007
PspFI CCCAGC 1 cut(s) 102
PspN4I GGNNCC 6 cut(s) 127, 160, 765, 909, 1008, 1009
PspPI GGNCC 3 cut(s) 40, 648, 1007
PspPPI RGGWCCY 1 cut(s) 1007
PsuI RGATCY 1 cut(s) 1037
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 8 cut(s) 85, 198, 300, 492, 495, 504, 507, 544
Sau3AI GATC 6 cut(s) 6, 142, 276, 369, 691, 1037
Sau96I GGNCC 3 cut(s) 40, 648, 1007
SchI GAGTC 3 cut(s) 99, 422, 986
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 214
Sfr303I CCGCGG 1 cut(s) 88
SgrBI CCGCGG 1 cut(s) 88
SinI GGWCC 2 cut(s) 648, 1007
SspMI CTAG 1 cut(s) 50
StyI CCWWGG 2 cut(s) 633, 828
TaaI ACNGT 3 cut(s) 610, 878, 986
TaiI ACGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 147
TauI GCSGC 5 cut(s) 87, 302, 494, 497, 546
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 4 cut(s) 13, 73, 304, 604
TseI GCWGC 3 cut(s) 197, 503, 506
Tsp45I GTSAC 4 cut(s) 13, 73, 304, 604
TspDTI ATGAA 5 cut(s) 370, 705, 906, 936, 1101
TspGWI ACGGA 5 cut(s) 151, 271, 318, 357, 1030
TspRI CASTG 1 cut(s) 597
VpaK11BI GGWCC 2 cut(s) 648, 1007
XapI RAATTY 4 cut(s) 182, 345, 565, 933
XmnI GAANNNNTTC 2 cut(s) 234, 933
XspI CTAG 1 cut(s) 50
Zsp2I ATGCAT 1 cut(s) 1086
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.