MD08G1040300.v1.1

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009896
Physical Location & Seq
Forward (+)
2984113 .. 2986993
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1040300.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGGCTATTCGTGTCACCCTGAGCTTCTCCGGCTATGTGGCCCAAAACCTTGCATCCTCGGCAAACCTCCGCGCGGGGAACTGCCGAGGATTTCACGAATGCTGGGTCAGGTCTCGGGTATTAGGATCCAGCCAGAAGCCCGATATCAACCCCTCTGTCCCCGTCCACAATTACCAGACCCAATTCTCCCGCTCGAAGCATTCGTCGGCCGCCGTTAGGCCCTTGCCGTCGCTGTACACCGCCCTCGCCGAGGAGATTCTAGGCGAGAGCAGCAAGAGCCCGATCTTTCTGGGGTTGATCTCGCTTCTGAAGTCAACGGCTATCGCCACCGGCGTCTCTTCGGCTCCGGCCGCCATGGGGATTTCGCCGTTTAATGTCAGTTCGATCATGCCCTCTTTACAGGCATCGAAGTGGCTGCCCAGCAACGACACCGTCCCGGAATCGATAATGAAGGAAGTGGATAAAGGTGGAACCTTGTGCGTGGACGAGGTTGCTCAGGCCTCGCAGCTGGCTAAGAAAGACTTGGGCAGGACCGGGTTTTTGTCACGATTGTTGAATTCTTGCTCGGAGGACGCTAAGGCTGTGTTCACTGCTGTTACAGTAAGTGTATTGTTTAAGTCCTTCCTGGCTGAGCCCCGATCGATTCCCTCCACTTCAATGTATCCCACTCTCGATGTGGGTGACCGAGTTTTGGCGGAGAAGGTTTCATATTTCTTTAAAAATCCTGAGGTCTCAGATATAGTAATTTTCAAGGCACCTCCAATCTTACAGGAAATTGGTTACAACTCAACCGATGTATTCATCAAAAGGATTGTAGCTAAGGCTGGAGATTGGGTCGAGGTTCGCAATGGGAAATTGCTTGTCAATGATCTTGTTCAGAATGAAAATTACATATTGGAGCCGCTCAATTATGAAATGGATCCAGTGCTTGTTCCAGAAGGCTATGTCTTCGTATTGGGAGATAATCGCAACAATAGTTTTGACTCTCATAACTGGGGTCCACTTCCTGTGAAAAACATTCTTGGCAGATCGGTATTTCGCTACTGGCCTCCTTCCAAGGTGTCTGACACCACTTTTGAACCACAAGGACCAAATAACGACGTTGCAGTTTCTTGA

Protein Analysis

372

Amino Acids

40.47

Weight (kDa)

6.91

Isoelectric Point (pI)

45.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 192 - 349 1.1e-42 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 754
AccBSI CCGCTC 2 cut(s) 192, 904
AccII CGCG 2 cut(s) 72, 74
AciI CCGC 8 cut(s) 70, 74, 190, 210, 240, 351, 695, 902
AclWI GGATC 4 cut(s) 120, 133, 914, 927
AcoI YGGCCR 2 cut(s) 207, 348
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 333
AfaI GTAC 1 cut(s) 236
AfiI CCNNNNNNNGG 5 cut(s) 73, 216, 250, 357, 691
AgsI TTSAA 4 cut(s) 556, 657, 751, 1079
AjnI CCWGG 1 cut(s) 624
AluBI AGCT 3 cut(s) 24, 508, 818
AluI AGCT 3 cut(s) 24, 508, 818
Alw26I GTCTC 3 cut(s) 117, 340, 736
AlwI GGATC 4 cut(s) 120, 133, 914, 927
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 6 cut(s) 39, 207, 218, 348, 498, 1046
ApeKI GCWGC 3 cut(s) 270, 415, 505
ApoI RAATTY 1 cut(s) 556
ArsI GACNNNNNNTTYG 8 cut(s) 90, 122, 188, 220, 523, 555, 1058, 1090
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 5 cut(s) 40, 219, 531, 998, 1088
AsuC2I CCSGG 2 cut(s) 437, 535
AsuHPI GGTGA 2 cut(s) 7, 692
AvaI CYCGRG 1 cut(s) 114
AvaII GGWCC 3 cut(s) 531, 998, 1088
AxyI CCTNAGG 1 cut(s) 726
BamHI GGATCC 2 cut(s) 125, 919
BanI GGYRCC 1 cut(s) 754
BanII GRGCYC 2 cut(s) 281, 636
BbsI GAAGAC 1 cut(s) 940
BbvI GCAGC 3 cut(s) 282, 402, 517
BceAI ACGGC 4 cut(s) 197, 211, 333, 352
BcgI CGANNNNNNTGC 2 cut(s) 397, 431
BciT130I CCWGG 1 cut(s) 626
BciVI GTATCC 1 cut(s) 672
BcnI CCSGG 2 cut(s) 437, 535
BcoDI GTCTC 3 cut(s) 117, 340, 736
BfaI CTAG 1 cut(s) 260
BfuI GTATCC 1 cut(s) 672
BisI GCNGC 6 cut(s) 210, 271, 351, 416, 506, 902
BlpI GCTNAGC 1 cut(s) 630
BlsI GCNGC 6 cut(s) 211, 272, 352, 417, 507, 903
Bme1390I CCNGG 3 cut(s) 437, 535, 626
Bme18I GGWCC 3 cut(s) 531, 998, 1088
BmeT110I CYCGRG 1 cut(s) 114
BmgT120I GGNCC 5 cut(s) 40, 219, 531, 998, 1088
BmiI GGNNCC 7 cut(s) 127, 345, 472, 756, 900, 921, 999
BmrFI CCNGG 3 cut(s) 437, 535, 626
BmrI ACTGGG 1 cut(s) 1003
BmsI GCATC 2 cut(s) 62, 413
BmuI ACTGGG 1 cut(s) 1003
BpiI GAAGAC 1 cut(s) 940
BpmI CTGGAG 1 cut(s) 846
Bpu10I CCTNAGC 4 cut(s) 20, 495, 576, 819
Bpu1102I GCTNAGC 1 cut(s) 630
BpuMI CCSGG 2 cut(s) 437, 535
Bsa29I ATCGAT 2 cut(s) 443, 641
BsaHI GRCGYC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 117, 736
BsaJI CCNNGG 5 cut(s) 57, 85, 249, 354, 1056
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 216, 250, 357, 691
Bse118I RCCGGY 1 cut(s) 329
Bse1I ACTGG 3 cut(s) 923, 998, 1049
Bse21I CCTNAGG 1 cut(s) 726
Bse3DI GCAATG 1 cut(s) 853
BseBI CCWGG 1 cut(s) 626
BseCI ATCGAT 2 cut(s) 443, 641
BseDI CCNNGG 5 cut(s) 57, 85, 249, 354, 1056
BseGI GGATG 1 cut(s) 53
BseLI CCNNNNNNNGG 5 cut(s) 73, 216, 250, 357, 691
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 5 cut(s) 11, 509, 621, 717, 747
BseNI ACTGG 3 cut(s) 923, 998, 1049
BseRI GAGGAG 1 cut(s) 266
BseX3I CGGCCG 2 cut(s) 207, 348
BseXI GCAGC 3 cut(s) 282, 402, 517
BseYI CCCAGC 2 cut(s) 102, 419
Bsh1236I CGCG 2 cut(s) 72, 74
Bsh1285I CGRYCG 3 cut(s) 210, 351, 641
BshFI GGCC 6 cut(s) 41, 209, 220, 350, 500, 1048
BshNI GGYRCC 1 cut(s) 754
BshVI ATCGAT 2 cut(s) 443, 641
BsiEI CGRYCG 3 cut(s) 210, 351, 641
BsiHKCI CYCGRG 1 cut(s) 114
BsiSI CCGG 5 cut(s) 30, 330, 347, 437, 534
BslFI GGGAC 2 cut(s) 143, 419
BslI CCNNNNNNNGG 5 cut(s) 73, 216, 250, 357, 691
BsmAI GTCTC 3 cut(s) 117, 340, 736
BsmBI CGTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 143, 419
BsmI GAATGC 2 cut(s) 104, 199
BsnI GGCC 6 cut(s) 41, 209, 220, 350, 500, 1048
Bso31I GGTCTC 2 cut(s) 117, 736
BsoBI CYCGRG 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 281, 636
Bsp1407I TGTACA 1 cut(s) 234
Bsp143I GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
Bsp1720I GCTNAGC 1 cut(s) 630
Bsp19I CCATGG 1 cut(s) 354
BspACI CCGC 8 cut(s) 70, 74, 190, 210, 240, 351, 695, 902
BspANI GGCC 6 cut(s) 41, 209, 220, 350, 500, 1048
BspCNI CTCAG 5 cut(s) 12, 508, 622, 718, 746
BspDI ATCGAT 2 cut(s) 443, 641
BspFNI CGCG 2 cut(s) 72, 74
BspLI GGNNCC 7 cut(s) 127, 345, 472, 756, 900, 921, 999
BspPI GGATC 4 cut(s) 120, 133, 914, 927
BspT107I GGYRCC 1 cut(s) 754
BspTNI GGTCTC 2 cut(s) 117, 736
BsrBI CCGCTC 2 cut(s) 192, 904
BsrDI GCAATG 1 cut(s) 853
BsrFI RCCGGY 1 cut(s) 329
BsrGI TGTACA 1 cut(s) 234
BsrI ACTGG 3 cut(s) 923, 998, 1049
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 5 cut(s) 57, 85, 249, 354, 1056
BssMI GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
BssNI GRCGYC 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 354, 1056
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 2 cut(s) 433, 601
Bst6I CTCTTC 1 cut(s) 343
BstACI GRCGYC 1 cut(s) 333
BstAUI TGTACA 1 cut(s) 234
BstC8I GCNNGC 1 cut(s) 510
BstDEI CTNAG 8 cut(s) 20, 495, 513, 576, 630, 726, 733, 819
BstDSI CCRYGG 1 cut(s) 354
BstEII GGTNACC 1 cut(s) 680
BstENI CCTNNNNNAGG 1 cut(s) 248
BstF5I GGATG 1 cut(s) 53
BstFNI CGCG 2 cut(s) 72, 74
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 8 cut(s) 128, 285, 300, 387, 641, 871, 922, 1031
BstMAI GTCTC 3 cut(s) 117, 340, 736
BstMBI GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
BstMCI CGRYCG 3 cut(s) 210, 351, 641
BstMWI GCNNNNNNNGC 5 cut(s) 30, 59, 270, 276, 350
BstNI CCWGG 1 cut(s) 626
BstPI GGTNACC 1 cut(s) 680
BstSCI CCNGG 3 cut(s) 435, 533, 624
BstUI CGCG 2 cut(s) 72, 74
BstV1I GCAGC 3 cut(s) 282, 402, 517
BstV2I GAAGAC 1 cut(s) 940
BstX2I RGATCY 2 cut(s) 125, 919
BstYI RGATCY 2 cut(s) 125, 919
BstZI CGGCCG 2 cut(s) 207, 348
Bsu15I ATCGAT 2 cut(s) 443, 641
Bsu36I CCTNAGG 1 cut(s) 726
BsuI GTATCC 1 cut(s) 672
BsuRI GGCC 6 cut(s) 41, 209, 220, 350, 500, 1048
BsuTUI ATCGAT 2 cut(s) 443, 641
BtgI CCRYGG 1 cut(s) 354
BtsCI GGATG 1 cut(s) 53
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 588, 930
Cac8I GCNNGC 1 cut(s) 510
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 1 cut(s) 329
Cfr13I GGNCC 5 cut(s) 40, 219, 531, 998, 1088
ClaI ATCGAT 2 cut(s) 443, 641
CseI GACGC 2 cut(s) 322, 581
Csp6I GTAC 1 cut(s) 235
CviAII CATG 2 cut(s) 355, 388
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 8 cut(s) 20, 495, 513, 576, 630, 726, 733, 819
DpnI GATC 8 cut(s) 127, 284, 299, 386, 640, 870, 921, 1030
DpnII GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
DraI TTTAAA 1 cut(s) 718
EaeI YGGCCR 2 cut(s) 207, 348
EagI CGGCCG 2 cut(s) 207, 348
Eam1104I CTCTTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 343
EciI GGCGGA 1 cut(s) 710
EclXI CGGCCG 2 cut(s) 207, 348
Eco130I CCWWGG 2 cut(s) 354, 1056
Eco147I AGGCCT 1 cut(s) 500
Eco24I GRGCYC 2 cut(s) 281, 636
Eco31I GGTCTC 2 cut(s) 117, 736
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 3 cut(s) 531, 998, 1088
Eco52I CGGCCG 2 cut(s) 207, 348
Eco57I CTGAAG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 726
Eco88I CYCGRG 1 cut(s) 114
Eco91I GGTNACC 1 cut(s) 680
EcoNI CCTNNNNNAGG 1 cut(s) 248
EcoO109I RGGNCCY 1 cut(s) 219
EcoO65I GGTNACC 1 cut(s) 680
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 624
EcoRV GATATC 1 cut(s) 145
EcoT14I CCWWGG 2 cut(s) 354, 1056
EcoT38I GRGCYC 2 cut(s) 281, 636
ErhI CCWWGG 2 cut(s) 354, 1056
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 2 cut(s) 358, 391
FaiI YATR 9 cut(s) 36, 356, 389, 709, 740, 893, 912, 945, 990
FalI AAGNNNNNCTT 2 cut(s) 506, 538
FaqI GGGAC 2 cut(s) 143, 419
FatI CATG 2 cut(s) 354, 387
FauI CCCGC 2 cut(s) 67, 197
Fnu4HI GCNGC 6 cut(s) 210, 271, 351, 416, 506, 902
FokI GGATG 1 cut(s) 40
FriOI GRGCYC 2 cut(s) 281, 636
Fsp4HI GCNGC 6 cut(s) 210, 271, 351, 416, 506, 902
FspBI CTAG 1 cut(s) 260
GlaI GCGC 1 cut(s) 73
GluI GCNGC 6 cut(s) 210, 271, 351, 416, 506, 902
GsaI CCCAGC 2 cut(s) 106, 423
GsuI CTGGAG 1 cut(s) 846
HaeIII GGCC 6 cut(s) 41, 209, 220, 350, 500, 1048
HapII CCGG 5 cut(s) 30, 330, 347, 437, 534
HgaI GACGC 2 cut(s) 322, 581
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 333
Hin1II CATG 2 cut(s) 358, 391
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HinfI GANTC 4 cut(s) 256, 440, 643, 983
HpaII CCGG 5 cut(s) 30, 330, 347, 437, 534
HphI GGTGA 2 cut(s) 7, 692
Hpy166II GTNNAC 6 cut(s) 166, 237, 315, 484, 588, 1001
Hpy188I TCNGA 5 cut(s) 309, 568, 736, 879, 1066
Hpy188III TCNNGA 6 cut(s) 95, 546, 671, 725, 935, 1113
Hpy8I GTNNAC 6 cut(s) 166, 237, 315, 484, 588, 1001
Hpy99I CGWCG 3 cut(s) 208, 232, 1103
HpyAV CCTTC 5 cut(s) 445, 631, 694, 932, 1062
HpyCH4III ACNGT 2 cut(s) 433, 601
HpyCH4IV ACGT 1 cut(s) 1101
HpyCH4V TGCA 2 cut(s) 53, 1106
HpyF10VI GCNNNNNNNGC 5 cut(s) 30, 59, 270, 276, 350
HpyF3I CTNAG 8 cut(s) 20, 495, 513, 576, 630, 726, 733, 819
HpySE526I ACGT 1 cut(s) 1101
Hsp92I GRCGYC 1 cut(s) 333
Hsp92II CATG 2 cut(s) 358, 391
HspAI GCGC 1 cut(s) 72
Kzo9I GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
LmnI GCTCC 2 cut(s) 349, 898
Lsp1109I GCAGC 3 cut(s) 282, 402, 517
LweI GCATC 2 cut(s) 62, 413
MaeI CTAG 1 cut(s) 260
MaeII ACGT 1 cut(s) 1101
MaeIII GTNAC 5 cut(s) 13, 543, 595, 680, 779
MalI GATC 8 cut(s) 127, 284, 299, 386, 640, 870, 921, 1030
MbiI CCGCTC 2 cut(s) 192, 904
MboI GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
MboII GAAGA 2 cut(s) 330, 940
MflI RGATCY 2 cut(s) 125, 919
MhlI GDGCHC 2 cut(s) 281, 636
MluCI AATT 8 cut(s) 169, 182, 556, 744, 774, 854, 886, 907
MlyI GAGTC 1 cut(s) 977
MseI TTAA 3 cut(s) 372, 615, 717
MslI CAYNNNNRTG 2 cut(s) 409, 656
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 5 cut(s) 30, 330, 347, 437, 534
MspR9I CCNGG 3 cut(s) 437, 535, 626
Mva1269I GAATGC 2 cut(s) 104, 199
MvaI CCWGG 1 cut(s) 626
MvnI CGCG 2 cut(s) 72, 74
MwoI GCNNNNNNNGC 5 cut(s) 30, 59, 270, 276, 350
NciI CCSGG 2 cut(s) 437, 535
NcoI CCATGG 1 cut(s) 354
NdeII GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
NlaIII CATG 2 cut(s) 358, 391
NlaIV GGNNCC 7 cut(s) 127, 345, 472, 756, 900, 921, 999
NmeAIII GCCGAG 3 cut(s) 38, 110, 274
NmuCI GTSAC 3 cut(s) 13, 543, 680
PceI AGGCCT 1 cut(s) 500
PcsI WCGNNNNNNNCGW 2 cut(s) 200, 330
PctI GAATGC 2 cut(s) 104, 199
PfeI GAWTC 3 cut(s) 256, 440, 643
PflFI GACNNNGTC 1 cut(s) 431
PfoI TCCNGGA 1 cut(s) 435
PkrI GCNGC 6 cut(s) 211, 272, 352, 417, 507, 903
Ple19I CGATCG 1 cut(s) 641
PleI GAGTC 1 cut(s) 977
PpsI GAGTC 1 cut(s) 977
Psp6I CCWGG 1 cut(s) 624
PspEI GGTNACC 1 cut(s) 680
PspFI CCCAGC 2 cut(s) 102, 419
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 7 cut(s) 127, 345, 472, 756, 900, 921, 999
PspPI GGNCC 5 cut(s) 40, 219, 531, 998, 1088
PsuI RGATCY 2 cut(s) 125, 919
PsyI GACNNNGTC 1 cut(s) 431
PvuI CGATCG 1 cut(s) 641
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
RseI CAYNNNNRTG 2 cut(s) 409, 656
SaqAI TTAA 3 cut(s) 372, 615, 717
SatI GCNGC 6 cut(s) 210, 271, 351, 416, 506, 902
Sau3AI GATC 8 cut(s) 125, 282, 297, 384, 638, 868, 919, 1028
Sau96I GGNCC 5 cut(s) 40, 219, 531, 998, 1088
SchI GAGTC 1 cut(s) 977
ScrFI CCNGG 3 cut(s) 437, 535, 626
SduI GDGCHC 2 cut(s) 281, 636
SfaNI GCATC 2 cut(s) 62, 413
SgrAI CRCCGGYG 1 cut(s) 329
SinI GGWCC 3 cut(s) 531, 998, 1088
SmiMI CAYNNNNRTG 2 cut(s) 409, 656
Sse9I AATT 8 cut(s) 169, 182, 556, 744, 774, 854, 886, 907
SseBI AGGCCT 1 cut(s) 500
SsiI CCGC 8 cut(s) 70, 74, 190, 210, 240, 351, 695, 902
SspMI CTAG 1 cut(s) 260
StuI AGGCCT 1 cut(s) 500
StyD4I CCNGG 3 cut(s) 435, 533, 624
StyI CCWWGG 2 cut(s) 354, 1056
TaaI ACNGT 2 cut(s) 433, 601
TaiI ACGT 1 cut(s) 1104
TaqI TCGA 7 cut(s) 194, 383, 407, 443, 641, 672, 837
TaqII GACCGA 1 cut(s) 699
TasI AATT 8 cut(s) 169, 182, 556, 744, 774, 854, 886, 907
TatI WGTACW 1 cut(s) 234
TauI GCSGC 3 cut(s) 212, 353, 904
TfiI GAWTC 3 cut(s) 256, 440, 643
Tru1I TTAA 3 cut(s) 372, 615, 717
Tru9I TTAA 3 cut(s) 372, 615, 717
TscAI CASTG 2 cut(s) 595, 930
TseFI GTSAC 3 cut(s) 13, 543, 680
TseI GCWGC 3 cut(s) 270, 415, 505
Tsp45I GTSAC 3 cut(s) 13, 543, 680
TspDTI ATGAA 5 cut(s) 464, 696, 790, 897, 927
TspRI CASTG 2 cut(s) 595, 930
Tth111I GACNNNGTC 1 cut(s) 431
VpaK11BI GGWCC 3 cut(s) 531, 998, 1088
XagI CCTNNNNNAGG 1 cut(s) 248
XapI RAATTY 1 cut(s) 556
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XspI CTAG 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.