Rw6G035410

Thylakoidal processing peptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009896
Physical Location & Seq
Reverse (-)
59937683 .. 59942141
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035410.1

Sequence Viewer

Length: 2244 bp
ATGGCGATCCGCGTCACCCTAGGGTTTTCCGGCTACGTGGCCCAAAACCTGGCCTCCTCCGCCAGTATTCGCGCCAGTAATTGCCGCGGCGTCCACGAATGCTGGGTTAGGTCTCTGCTGTTCGGCACCAACCAGAAGGCCGATCTCGACCCCTCCGGCACCGTCCCCGGTTACCGGTCCAAATTCGCCGCCAGGTCCAAGGCTCCGACCTCCCTCTACGGCGCCCTTGCCGAAGAATTCCTCTGCCAAAGCTCCAAGACCCCGATTATTCCTTCTCTGGCTTCCCCGTCTCTTGGCCTCGGAATCTCGCCGGTCAAACCCGGTTCGATCATGCCGTTCTTGCAGGCCTCCAAGTGGCTTCCCTGCAACGAATCTGTTCCGGCGAAATTGGCGAGCGAGATGGACAGGAAACTGCGTTTGGATGATGATGATGCTGAGGTGGTGGCCTCTGCCTCGGAGTTGAGTAAGAATGGATTGCAGAGGAGCGGTTGGTTATCGCGCCTCTTGAATTCTTGCTCCGAAGACGCAAAGGCAGTCTTCACTGCTGTGACTGTCAGCGTTCTGTTCCGCTCATCCTTGGCCGAGCCCAGGTCCATTCCTTCCACCTCCATGTACCCTACTCTCGATGTGGGTGATCGTGTTCTAGCTGAGAAGGTTTCATATCTGTTTAGAAAGCCTGAGGTTTCAGATATAGTCATTTTCAAGGCACCTCCAATCTTGCAGGAGATTGGCTTTAGCTCGGGCGATGTATTCATAAAAAGAATTGTGGCCAAGGCTGGAGACTATGTTGAGGTACATGATGGGAAATTGTTGGTGAACAGTGAAATTCAAAATGAAAATTACATATTGGAGCCTCTTGCCTATGAAATGGAACCAATTCTCATTCCAGAAGGCTATGTCTTTGTGATGGGGGACAACCGCAACAACAGTTTTGACTCTCATAACTGGGGTCCCCTTCCTGTAAAAAACATTCTTGGAAGATCCGTGTTTCGCTACTGGCCTCCCTCCAAAGTGTCCGACATTATGCATGAACCACAGGTAGAAAAATCTTACAAAACAATGAGCAGAAGCAGTGGCGGTGTCCCAATCTCCGTCCTCCTCTTCCTTTACCTCCTCCAATTTCCCCTCCAATTCATTCTAGCTTTTAATTCCTCCCACATCTTGCAGGACGTCTTGAAGCAAATCACGGTCGAGCAGAGGTGGGATTTGCAGAGCGTTAGGATTTCCAGATTGGATACCGGCAAAGTACGCTTCGGGAGTGCCCAGAGCTACGAATTCCGGATCGGATTCGGCAAGTCCCACGTCGTCGTCTGGTTCTCCGACGACGTCGCTTCTTGGAGAAAGTTCCGCAAACCAAGAACCCATTTTGCCTCTCTTGTCAAGGAGCTCAGTTCCATGGCTGCAGTTGACACCTTCAAAGTGGAGGGGCCGTTTGAGTTACGAGTCGGTTCAGACCATGACCTCTCCCTTTCATTGCCGATGAACACAACGTATAGTAGAGGGTTGAAAAGGGTGCTTGTTGGTGAAGGCATTACTGTTGAGGTAACGAGAGCAACAGAAGTTTCTGTGTTTCATGCATCTGATCTTGGTGTGGCGATGAACGGAAGTGATACTATTGACAAGGAGAAAAGTGAGTTTTGGCCATTTTGGCATTCATATTGCGCGCCATTAGTTCCCATTCGCGTTTTAGGGCCTGCATCGTTGGTTGCATACAGGACTAGAAATCCTGCTGCTCACATTGAAACTAAATTTATGTCCAAGGATATAATTGAGTTGTTGCCAGAGAAATGCTTCAGCAGTCATCGTACATACAAGAAGCAGAAGCAGTCTTGCCCCATTGATTCTTTAAGTTTGAAAATATCTATGCTGGAAAGAATTTGGAAAGGCGTTCTTGGTGATAGGATTCGACAAAAAGGACTGTCCGGATTTCTTGAAGCAAAAATCAAAGCCTCAACTATTGTTCGGTTCAAGCTTCAGCTGGAAAGGGATTTTAGGAGGAAGGGGATACTCCAGCAGGACATTGCGGGATGGAGGACCAGGTCTGCTGTGGAAAGGGTTTGGTTTGAAGTTTTGGCGACAGTTGAATTAGAGAGGTTGAGGCTTAGCCCTATCTTGGTTAAAAAGATAAGGCCATTCATTATAGCAGATTCAGTTGAATGGGATAATCCTTCGAATATTTCCTTTACCAAATCCCAATCTATCCTTGTCGCTTCTGAAGCGCTTACGTTGGATGTGAAATGGTAG

Protein Analysis

747

Amino Acids

83.57

Weight (kDa)

9.26

Isoelectric Point (pI)

48.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S26 PF10502 176 - 333 9.1e-42 Signal peptidase, peptidase S26
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1173, 1329
AccB1I GGYRCC 4 cut(s) 125, 158, 221, 706
AccB7I CCANNNNNTGG 1 cut(s) 49
AccBSI CCGCTC 2 cut(s) 486, 570
AccII CGCG 6 cut(s) 12, 72, 87, 499, 1664, 1683
AccIII TCCGGA 2 cut(s) 1278, 1922
AclWI GGATC 2 cut(s) 975, 1289
AcoI YGGCCR 3 cut(s) 579, 768, 1640
AcsI RAATTY 7 cut(s) 182, 236, 508, 825, 1274, 1748, 1875
AcuI CTGAAG 3 cut(s) 1777, 1958, 2235
AcyI GRCGYC 4 cut(s) 90, 222, 1170, 1326
AfaI GTAC 4 cut(s) 614, 795, 1248, 1807
AfeI AGCGCT 1 cut(s) 2220
AfiI CCNNNNNNNGG 6 cut(s) 49, 174, 293, 354, 588, 2113
AgeI ACCGGT 1 cut(s) 174
AjiI CACGTC 1 cut(s) 1303
AjnI CCWGG 4 cut(s) 48, 191, 587, 2036
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AleI CACNNNNGTG 1 cut(s) 545
AluBI AGCT 8 cut(s) 252, 647, 738, 1142, 1269, 1387, 1972, 1978
AluI AGCT 8 cut(s) 252, 647, 738, 1142, 1269, 1387, 1972, 1978
Alw21I GWGCWC 1 cut(s) 1389
Alw26I GTCTC 3 cut(s) 117, 294, 774
AlwI GGATC 2 cut(s) 975, 1289
Ama87I CYCGRG 1 cut(s) 739
Aor13HI TCCGGA 2 cut(s) 1278, 1922
Aor51HI AGCGCT 1 cut(s) 2220
ApeKI GCWGC 2 cut(s) 1400, 1730
ApoI RAATTY 7 cut(s) 182, 236, 508, 825, 1274, 1748, 1875
AsiGI ACCGGT 1 cut(s) 174
Asp700I GAANNNNTTC 3 cut(s) 375, 876, 1790
AspA2I CCTAGG 1 cut(s) 19
AspLEI GCGC 6 cut(s) 74, 224, 501, 1664, 1666, 2221
AspS9I GGNCC 8 cut(s) 40, 177, 195, 591, 950, 1427, 1691, 2034
AsuC2I CCSGG 2 cut(s) 168, 321
AsuHPI GGTGA 5 cut(s) 7, 644, 826, 1535, 1907
AsuII TTCGAA 1 cut(s) 2171
AvaI CYCGRG 1 cut(s) 739
AvaII GGWCC 5 cut(s) 177, 195, 591, 950, 2034
AvrII CCTAGG 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 678
BaeGI GKGCMC 1 cut(s) 1264
BalI TGGCCA 2 cut(s) 770, 1642
BanI GGYRCC 4 cut(s) 125, 158, 221, 706
BanII GRGCYC 2 cut(s) 588, 1389
BbsI GAAGAC 2 cut(s) 528, 529
Bbv12I GWGCWC 1 cut(s) 1389
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 2 cut(s) 1387, 1717
BccI CCATC 4 cut(s) 394, 794, 901, 2022
BceAI ACGGC 3 cut(s) 235, 319, 1414
BciT130I CCWGG 4 cut(s) 50, 193, 589, 2038
BciVI GTATCC 2 cut(s) 1228, 1998
BcnI CCSGG 2 cut(s) 168, 321
BcoDI GTCTC 3 cut(s) 117, 294, 774
BfaI CTAG 4 cut(s) 20, 644, 1139, 1719
BfmI CTRYAG 1 cut(s) 1401
BfoI RGCGCY 2 cut(s) 225, 2222
BfuI GTATCC 2 cut(s) 1228, 1998
BglI GCCNNNNNGGC 1 cut(s) 1648
BisI GCNGC 5 cut(s) 85, 88, 189, 1401, 1731
BlnI CCTAGG 1 cut(s) 19
BlpI GCTNAGC 1 cut(s) 2102
BlsI GCNGC 5 cut(s) 86, 89, 190, 1402, 1732
Bme1390I CCNGG 6 cut(s) 50, 168, 193, 321, 589, 2038
Bme18I GGWCC 5 cut(s) 177, 195, 591, 950, 2034
BmeT110I CYCGRG 1 cut(s) 739
BmgBI CACGTC 1 cut(s) 1303
BmgT120I GGNCC 8 cut(s) 40, 177, 195, 591, 950, 1427, 1691, 2034
BmrFI CCNGG 6 cut(s) 50, 168, 193, 321, 589, 2038
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 3 cut(s) 421, 1586, 1706
BmuI ACTGGG 1 cut(s) 955
BpiI GAAGAC 2 cut(s) 528, 529
BpmI CTGGAG 2 cut(s) 798, 1994
Bpu10I CCTNAGC 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 2102
Bpu14I TTCGAA 1 cut(s) 2171
BpuMI CCSGG 2 cut(s) 168, 321
BsaAI YACGTR 1 cut(s) 37
BsaHI GRCGYC 4 cut(s) 90, 222, 1170, 1326
BsaI GGTCTC 1 cut(s) 117
BsaWI WCCGGW 3 cut(s) 174, 1278, 1922
BsaXI ACNNNNNCTCC 6 cut(s) 38, 68, 771, 801, 1415, 1445
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 174, 293, 354, 588, 2113
Bse118I RCCGGY 3 cut(s) 174, 310, 1238
Bse1I ACTGG 4 cut(s) 63, 75, 950, 1001
Bse21I CCTNAGG 1 cut(s) 678
Bse3DI GCAATG 2 cut(s) 1472, 2019
BseAI TCCGGA 2 cut(s) 1278, 1922
BseBI CCWGG 4 cut(s) 50, 193, 589, 2038
BseGI GGATG 4 cut(s) 427, 572, 2033, 2236
BseLI CCNNNNNNNGG 6 cut(s) 49, 174, 293, 354, 588, 2113
BseMI GCAATG 2 cut(s) 1472, 2019
BseMII CTCAG 4 cut(s) 426, 639, 669, 1402
BseNI ACTGG 4 cut(s) 63, 75, 950, 1001
BsePI GCGCGC 1 cut(s) 1662
BseRI GAGGAG 4 cut(s) 46, 496, 1088, 1103
BseSI GKGCMC 1 cut(s) 1264
BseXI GCAGC 2 cut(s) 1387, 1717
BseYI CCCAGC 1 cut(s) 102
Bsh1236I CGCG 6 cut(s) 12, 72, 87, 499, 1664, 1683
Bsh1285I CGRYCG 1 cut(s) 1191
BshNI GGYRCC 4 cut(s) 125, 158, 221, 706
BshTI ACCGGT 1 cut(s) 174
BsiEI CGRYCG 1 cut(s) 1191
BsiHKAI GWGCWC 1 cut(s) 1389
BsiHKCI CYCGRG 1 cut(s) 739
BslFI GGGAC 5 cut(s) 149, 926, 936, 1067, 1282
BslI CCNNNNNNNGG 6 cut(s) 49, 174, 293, 354, 588, 2113
BsmAI GTCTC 3 cut(s) 117, 294, 774
BsmBI CGTCTC 1 cut(s) 294
BsmFI GGGAC 5 cut(s) 149, 926, 936, 1067, 1282
BsmI GAATGC 2 cut(s) 104, 1651
Bso31I GGTCTC 1 cut(s) 117
BsoBI CYCGRG 1 cut(s) 739
Bsp119I TTCGAA 1 cut(s) 2171
Bsp1286I GDGCHC 3 cut(s) 588, 1264, 1389
Bsp13I TCCGGA 2 cut(s) 1278, 1922
Bsp143I GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
Bsp1720I GCTNAGC 1 cut(s) 2102
Bsp19I CCATGG 1 cut(s) 1395
BspCNI CTCAG 4 cut(s) 427, 640, 670, 1401
BspEI TCCGGA 2 cut(s) 1278, 1922
BspFNI CGCG 6 cut(s) 12, 72, 87, 499, 1664, 1683
BspMAI CTGCAG 1 cut(s) 1405
BspPI GGATC 2 cut(s) 975, 1289
BspT104I TTCGAA 1 cut(s) 2171
BspT107I GGYRCC 4 cut(s) 125, 158, 221, 706
BspTNI GGTCTC 1 cut(s) 117
BsrBI CCGCTC 2 cut(s) 486, 570
BsrDI GCAATG 2 cut(s) 1472, 2019
BsrFI RCCGGY 3 cut(s) 174, 310, 1238
BsrI ACTGG 4 cut(s) 63, 75, 950, 1001
BssAI RCCGGY 3 cut(s) 174, 310, 1238
BssHII GCGCGC 1 cut(s) 1662
BssMI GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
BssNI GRCGYC 4 cut(s) 90, 222, 1170, 1326
BssT1I CCWWGG 6 cut(s) 19, 198, 576, 771, 1395, 1758
Bst2UI CCWGG 4 cut(s) 50, 193, 589, 2038
Bst4CI ACNGT 8 cut(s) 163, 553, 821, 929, 1189, 1537, 1920, 2080
Bst6I CTCTTC 1 cut(s) 1106
BstACI GRCGYC 4 cut(s) 90, 222, 1170, 1326
BstBAI YACGTR 1 cut(s) 37
BstBI TTCGAA 1 cut(s) 2171
BstC8I GCNNGC 4 cut(s) 345, 394, 1664, 1695
BstDEI CTNAG 5 cut(s) 435, 648, 678, 1388, 2102
BstDSI CCRYGG 2 cut(s) 85, 1395
BstEII GGTNACC 1 cut(s) 170
BstF5I GGATG 4 cut(s) 427, 572, 2033, 2236
BstFNI CGCG 6 cut(s) 12, 72, 87, 499, 1664, 1683
BstH2I RGCGCY 2 cut(s) 225, 2222
BstHHI GCGC 6 cut(s) 74, 224, 501, 1664, 1666, 2221
BstKTI GATC 7 cut(s) 9, 145, 330, 637, 983, 1284, 1585
BstMAI GTCTC 3 cut(s) 117, 294, 774
BstMBI GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
BstMCI CGRYCG 1 cut(s) 1191
BstMWI GCNNNNNNNGC 6 cut(s) 59, 340, 389, 1248, 1648, 2216
BstNI CCWGG 4 cut(s) 50, 193, 589, 2038
BstPI GGTNACC 1 cut(s) 170
BstSCI CCNGG 6 cut(s) 48, 166, 191, 319, 587, 2036
BstSFI CTRYAG 1 cut(s) 1401
BstSLI GKGCMC 1 cut(s) 1264
BstUI CGCG 6 cut(s) 12, 72, 87, 499, 1664, 1683
BstV1I GCAGC 2 cut(s) 1387, 1717
BstV2I GAAGAC 2 cut(s) 528, 529
BstX2I RGATCY 1 cut(s) 980
BstYI RGATCY 1 cut(s) 980
Bsu36I CCTNAGG 1 cut(s) 678
BsuI GTATCC 2 cut(s) 1228, 1998
BtgI CCRYGG 2 cut(s) 85, 1395
BtgZI GCGATG 2 cut(s) 759, 1610
BtrI CACGTC 1 cut(s) 1303
BtsCI GGATG 4 cut(s) 427, 572, 2033, 2236
BtsI GCAGTG 2 cut(s) 540, 1078
BtsIMutI CAGTG 3 cut(s) 540, 826, 1078
Cac8I GCNNGC 4 cut(s) 345, 394, 1664, 1695
CfoI GCGC 6 cut(s) 74, 224, 501, 1664, 1666, 2221
Cfr10I RCCGGY 3 cut(s) 174, 310, 1238
Cfr13I GGNCC 8 cut(s) 40, 177, 195, 591, 950, 1427, 1691, 2034
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 2 cut(s) 79, 533
CsiI ACCWGGT 1 cut(s) 2036
Csp6I GTAC 4 cut(s) 613, 794, 1247, 1806
CspAI ACCGGT 1 cut(s) 174
CviAII CATG 7 cut(s) 331, 610, 797, 1028, 1396, 1457, 1574
CviQI GTAC 4 cut(s) 613, 794, 1247, 1806
DdeI CTNAG 5 cut(s) 435, 648, 678, 1388, 2102
DinI GGCGCC 1 cut(s) 223
DpnI GATC 7 cut(s) 8, 144, 329, 636, 982, 1283, 1584
DpnII GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
EaeI YGGCCR 3 cut(s) 579, 768, 1640
Eam1104I CTCTTC 1 cut(s) 1106
EarI CTCTTC 1 cut(s) 1106
EciI GGCGGA 1 cut(s) 49
Ecl136II GAGCTC 1 cut(s) 1387
Eco130I CCWWGG 6 cut(s) 19, 198, 576, 771, 1395, 1758
Eco147I AGGCCT 1 cut(s) 347
Eco24I GRGCYC 2 cut(s) 588, 1389
Eco31I GGTCTC 1 cut(s) 117
Eco47I GGWCC 5 cut(s) 177, 195, 591, 950, 2034
Eco47III AGCGCT 1 cut(s) 2220
Eco53kI GAGCTC 1 cut(s) 1387
Eco57I CTGAAG 3 cut(s) 1777, 1958, 2235
Eco81I CCTNAGG 1 cut(s) 678
Eco88I CYCGRG 1 cut(s) 739
Eco91I GGTNACC 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 1387
EcoO109I RGGNCCY 2 cut(s) 950, 1691
EcoO65I GGTNACC 1 cut(s) 170
EcoRI GAATTC 3 cut(s) 236, 508, 1274
EcoRII CCWGG 4 cut(s) 48, 191, 587, 2036
EcoT14I CCWWGG 6 cut(s) 19, 198, 576, 771, 1395, 1758
EcoT22I ATGCAT 2 cut(s) 1029, 1579
EcoT38I GRGCYC 2 cut(s) 588, 1389
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 6 cut(s) 19, 198, 576, 771, 1395, 1758
Esp3I CGTCTC 1 cut(s) 294
FaeI CATG 7 cut(s) 334, 613, 800, 1031, 1399, 1460, 1577
FalI AAGNNNNNCTT 4 cut(s) 521, 553, 1875, 1907
FaqI GGGAC 5 cut(s) 149, 926, 936, 1067, 1282
FatI CATG 7 cut(s) 330, 609, 796, 1027, 1395, 1456, 1573
FauI CCCGC 1 cut(s) 2017
Fnu4HI GCNGC 5 cut(s) 85, 88, 189, 1401, 1731
FokI GGATG 3 cut(s) 434, 559, 2040
FriOI GRGCYC 2 cut(s) 588, 1389
Fsp4HI GCNGC 5 cut(s) 85, 88, 189, 1401, 1731
FspBI CTAG 4 cut(s) 20, 644, 1139, 1719
GlaI GCGC 6 cut(s) 73, 223, 500, 1663, 1665, 2220
GluI GCNGC 5 cut(s) 85, 88, 189, 1401, 1731
GsaI CCCAGC 1 cut(s) 106
GsuI CTGGAG 2 cut(s) 798, 1994
HaeII RGCGCY 2 cut(s) 225, 2222
HgaI GACGC 2 cut(s) 79, 533
HhaI GCGC 6 cut(s) 74, 224, 501, 1664, 1666, 2221
Hin1I GRCGYC 4 cut(s) 90, 222, 1170, 1326
Hin1II CATG 7 cut(s) 334, 613, 800, 1031, 1399, 1460, 1577
Hin6I GCGC 6 cut(s) 72, 222, 499, 1662, 1664, 2219
HinP1I GCGC 6 cut(s) 72, 222, 499, 1662, 1664, 2219
HincII GTYRAC 1 cut(s) 1408
HindII GTYRAC 1 cut(s) 1408
HindIII AAGCTT 1 cut(s) 1970
HinfI GANTC 8 cut(s) 303, 371, 935, 1287, 1443, 1841, 1903, 2147
HphI GGTGA 5 cut(s) 7, 644, 826, 1535, 1907
Hpy166II GTNNAC 3 cut(s) 94, 817, 1408
Hpy8I GTNNAC 3 cut(s) 94, 817, 1408
Hpy99I CGWCG 5 cut(s) 1307, 1310, 1325, 1328, 1331
HpyCH4III ACNGT 8 cut(s) 163, 553, 821, 929, 1189, 1537, 1920, 2080
HpyCH4IV ACGT 6 cut(s) 36, 1170, 1302, 1326, 1490, 2225
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 340, 389, 1248, 1648, 2216
HpyF3I CTNAG 5 cut(s) 435, 648, 678, 1388, 2102
HpySE526I ACGT 6 cut(s) 36, 1170, 1302, 1326, 1490, 2225
Hsp92I GRCGYC 4 cut(s) 90, 222, 1170, 1326
Hsp92II CATG 7 cut(s) 334, 613, 800, 1031, 1399, 1460, 1577
HspAI GCGC 6 cut(s) 72, 222, 499, 1662, 1664, 2219
KasI GGCGCC 1 cut(s) 221
KflI GGGWCCC 1 cut(s) 950
Kpn2I TCCGGA 2 cut(s) 1278, 1922
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
LmnI GCTCC 6 cut(s) 208, 257, 483, 521, 850, 1384
Lsp1109I GCAGC 2 cut(s) 1387, 1717
LweI GCATC 3 cut(s) 421, 1586, 1706
MabI ACCWGGT 1 cut(s) 2036
MaeI CTAG 4 cut(s) 20, 644, 1139, 1719
MaeII ACGT 6 cut(s) 36, 1170, 1302, 1326, 1490, 2225
MaeIII GTNAC 5 cut(s) 13, 170, 547, 1437, 1543
MalI GATC 7 cut(s) 8, 144, 329, 636, 982, 1283, 1584
MbiI CCGCTC 2 cut(s) 486, 570
MboI GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
MboII GAAGA 5 cut(s) 245, 529, 533, 990, 1093
MflI RGATCY 1 cut(s) 980
MhlI GDGCHC 3 cut(s) 588, 1264, 1389
MlsI TGGCCA 2 cut(s) 770, 1642
MluNI TGGCCA 2 cut(s) 770, 1642
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 2 cut(s) 929, 1452
MmeI TCCRAC 4 cut(s) 230, 1041, 1344, 2208
Mox20I TGGCCA 2 cut(s) 770, 1642
Mph1103I ATGCAT 2 cut(s) 1029, 1579
MroI TCCGGA 2 cut(s) 1278, 1922
MroXI GAANNNNTTC 3 cut(s) 375, 876, 1790
MscI TGGCCA 2 cut(s) 770, 1642
MseI TTAA 3 cut(s) 1146, 1847, 2118
MslI CAYNNNNRTG 2 cut(s) 545, 608
Msp20I TGGCCA 2 cut(s) 770, 1642
MspA1I CMGCKG 2 cut(s) 87, 1978
MspR9I CCNGG 6 cut(s) 50, 168, 193, 321, 589, 2038
Mva1269I GAATGC 2 cut(s) 104, 1651
MvaI CCWGG 4 cut(s) 50, 193, 589, 2038
MvnI CGCG 6 cut(s) 12, 72, 87, 499, 1664, 1683
MwoI GCNNNNNNNGC 6 cut(s) 59, 340, 389, 1248, 1648, 2216
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 2 cut(s) 168, 321
NcoI CCATGG 1 cut(s) 1395
NdeII GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
NlaIII CATG 7 cut(s) 334, 613, 800, 1031, 1399, 1460, 1577
NmeAIII GCCGAG 1 cut(s) 607
NmuCI GTSAC 2 cut(s) 13, 547
NsiI ATGCAT 2 cut(s) 1029, 1579
NspV TTCGAA 1 cut(s) 2171
OliI CACNNNNGTG 1 cut(s) 545
PauI GCGCGC 1 cut(s) 1662
PceI AGGCCT 1 cut(s) 347
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PctI GAATGC 2 cut(s) 104, 1651
PdmI GAANNNNTTC 3 cut(s) 375, 876, 1790
PfeI GAWTC 6 cut(s) 303, 371, 1287, 1841, 1903, 2147
PflFI GACNNNGTC 2 cut(s) 1325, 2038
PflMI CCANNNNNTGG 1 cut(s) 49
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 5 cut(s) 86, 89, 190, 1402, 1732
PleI GAGTC 2 cut(s) 929, 1451
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 2 cut(s) 929, 1451
Ppu21I YACGTR 1 cut(s) 37
PpuMI RGGWCCY 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 1389
Psp5II RGGWCCY 1 cut(s) 950
Psp6I CCWGG 4 cut(s) 48, 191, 587, 2036
PspEI GGTNACC 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 4 cut(s) 48, 191, 587, 2036
PspPI GGNCC 8 cut(s) 40, 177, 195, 591, 950, 1427, 1691, 2034
PspPPI RGGWCCY 1 cut(s) 950
PstI CTGCAG 1 cut(s) 1405
PsuI RGATCY 1 cut(s) 980
PsyI GACNNNGTC 2 cut(s) 1325, 2038
PteI GCGCGC 1 cut(s) 1662
PvuII CAGCTG 1 cut(s) 1978
RsaI GTAC 4 cut(s) 614, 795, 1248, 1807
RsaNI GTAC 4 cut(s) 613, 794, 1247, 1806
RseI CAYNNNNRTG 2 cut(s) 545, 608
SacI GAGCTC 1 cut(s) 1389
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 3 cut(s) 1146, 1847, 2118
SatI GCNGC 5 cut(s) 85, 88, 189, 1401, 1731
Sau3AI GATC 7 cut(s) 6, 142, 327, 634, 980, 1281, 1582
Sau96I GGNCC 8 cut(s) 40, 177, 195, 591, 950, 1427, 1691, 2034
SchI GAGTC 2 cut(s) 929, 1452
ScrFI CCNGG 6 cut(s) 50, 168, 193, 321, 589, 2038
SduI GDGCHC 3 cut(s) 588, 1264, 1389
SexAI ACCWGGT 1 cut(s) 2036
SfaNI GCATC 3 cut(s) 421, 1586, 1706
SfcI CTRYAG 1 cut(s) 1401
SfoI GGCGCC 1 cut(s) 223
Sfr303I CCGCGG 1 cut(s) 88
SfuI TTCGAA 1 cut(s) 2171
SgrBI CCGCGG 1 cut(s) 88
SinI GGWCC 5 cut(s) 177, 195, 591, 950, 2034
SmiMI CAYNNNNRTG 2 cut(s) 545, 608
SseBI AGGCCT 1 cut(s) 347
SspDI GGCGCC 1 cut(s) 221
SspI AATATT 1 cut(s) 2176
SspMI CTAG 4 cut(s) 20, 644, 1139, 1719
SstI GAGCTC 1 cut(s) 1389
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 6 cut(s) 48, 166, 191, 319, 587, 2036
StyI CCWWGG 6 cut(s) 19, 198, 576, 771, 1395, 1758
TaaI ACNGT 8 cut(s) 163, 553, 821, 929, 1189, 1537, 1920, 2080
TaiI ACGT 6 cut(s) 39, 1173, 1305, 1329, 1493, 2228
TaqI TCGA 6 cut(s) 147, 326, 624, 1191, 1906, 2171
TauI GCSGC 3 cut(s) 87, 90, 191
TfiI GAWTC 6 cut(s) 303, 371, 1287, 1841, 1903, 2147
Tru1I TTAA 3 cut(s) 1146, 1847, 2118
Tru9I TTAA 3 cut(s) 1146, 1847, 2118
TscAI CASTG 3 cut(s) 547, 826, 1078
TseFI GTSAC 2 cut(s) 13, 547
TseI GCWGC 2 cut(s) 1400, 1730
Tsp45I GTSAC 2 cut(s) 13, 547
TspGWI ACGGA 3 cut(s) 973, 1081, 1617
TspRI CASTG 3 cut(s) 547, 826, 1078
Tth111I GACNNNGTC 2 cut(s) 1325, 2038
Van91I CCANNNNNTGG 1 cut(s) 49
VpaK11BI GGWCC 5 cut(s) 177, 195, 591, 950, 2034
XapI RAATTY 7 cut(s) 182, 236, 508, 825, 1274, 1748, 1875
XcmI CCANNNNNNNNNTGG 1 cut(s) 2044
XmaJI CCTAGG 1 cut(s) 19
XmnI GAANNNNTTC 3 cut(s) 375, 876, 1790
XspI CTAG 4 cut(s) 20, 644, 1139, 1719
ZraI GACGTC 2 cut(s) 1171, 1327
Zsp2I ATGCAT 2 cut(s) 1029, 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.