AT1G09390

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005861
Physical Location & Seq
Forward (+)
3031046 .. 3033582
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G09390.1

Sequence Viewer

Length: 1113 bp
ATGGCTACTCTCTCTCTTCACTCCCACTCTTTCCTTCTTGTTCTCCTTCCGTTTATCCTCATTCTCCGACAAAACTTGGCAGTGGCCGGAGGATGTCAGGTTCCGCCGGTGATTTTTAACTTTGGCGATTCCAACTCCGACACGGGTGGACTCGTCGCCGGACTCGGATACAGCATTGGTCTTCCTAACGGTCGTTCGTTTTTCCAGAGATCCACCGGCCGATTATCCGACGGACGCCTCGTCATCGATTTCCTTTGCCAGAGTTTGAATACAAGTCTATTGAACCCATACTTAGACTCATTGGTTGGATCAAAGTTCCAAAACGGTGCCAATTTCGCAATTGTTGGATCTTCCACTCTTCCCAGATATGTTCCTTTTGCTCTCAACATTCAGCTCATGCAGTTCCTCCATTTCAAATCCCGAGCACTCGAGCTTGCTTCTATTTCAGATCCTTTGAAAGAGATGATGATAGGCGAAAGTGGATTCAGAAACGCATTGTACATGATCGATATCGGACAGAATGATATTGCGGATTCATTCTCCAAAGGCCTTTCTTACTCCCGGGTCGTCAAGCTTATTCCAAACGTTATTTCCGAGATCAAAAGTGCCATAAAGATATTGTATGATGAAGGAGGGAGGAAATTTTGGGTTCACAACACAGGACCATTAGGTTGTCTGCCTCAGAAACTTTCAATGGTTCATAGCAAAGGTTTTGATAAGCATGGTTGTCTAGCAACTTATAACGCAGCTGCAAAACTGTTTAACGAAGGACTGGATCATATGTGTCGGGATCTGAGGACGGAACTGAAAGAGGCCAACATTGTCTATGTCGACATTTATGCCATCAAGTACGATCTCATCGCAAACTCCAACAATTATGGCTTTGAGAAACCCTTAATGGCATGTTGCGGATACGGAGGGCCTCCTTACAACTACAATGTGAACATAACGTGTGGGAATGGTGGCTCTAAAAGTTGCGACGAAGGGTCTCGGTTTATAAGCTGGGACGGAATACACTACACCGAGACTGCTAACGCCATTGTAGCCATGAAAGTATTGTCGATGCAACATTCTACGCCACCAACTCCGTTCCATTTCTTCTGCGGCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

40.48

Weight (kDa)

7.99

Isoelectric Point (pI)

31.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 38 - 352 3.8e-56 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 741, 998
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 831
AciI CCGC 5 cut(s) 104, 530, 909, 1104, 1107
AclI AACGTT 1 cut(s) 585
AclWI GGATC 6 cut(s) 204, 316, 355, 443, 783, 798
AcoI YGGCCR 2 cut(s) 84, 217
AcsI RAATTY 1 cut(s) 641
AcyI GRCGYC 1 cut(s) 235
AfaI GTAC 2 cut(s) 500, 851
AflIII ACRYGT 1 cut(s) 950
AgsI TTSAA 5 cut(s) 268, 283, 415, 457, 693
AhdI GACNNNNNGTC 2 cut(s) 239, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 574, 606
AloI GAACNNNNNNTCC 2 cut(s) 84, 116
AluBI AGCT 5 cut(s) 394, 433, 574, 749, 1002
AluI AGCT 5 cut(s) 394, 433, 574, 749, 1002
Alw21I GWGCWC 1 cut(s) 427
Alw26I GTCTC 2 cut(s) 993, 1019
AlwI GGATC 6 cut(s) 204, 316, 355, 443, 783, 798
Ama87I CYCGRG 3 cut(s) 420, 428, 561
AoxI GGCC 5 cut(s) 84, 217, 547, 813, 920
ApeKI GCWGC 2 cut(s) 746, 749
ApoI RAATTY 1 cut(s) 641
AspS9I GGNCC 2 cut(s) 662, 920
AsuC2I CCSGG 2 cut(s) 562, 563
AsuHPI GGTGA 1 cut(s) 121
AvaI CYCGRG 3 cut(s) 420, 428, 561
AvaII GGWCC 1 cut(s) 662
BanI GGYRCC 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 427
BbvI GCAGC 2 cut(s) 736, 758
BccI CCATC 1 cut(s) 851
BcgI CGANNNNNNTGC 2 cut(s) 821, 855
BciVI GTATCC 2 cut(s) 161, 905
BcnI CCSGG 2 cut(s) 562, 563
BcoDI GTCTC 2 cut(s) 993, 1019
BfaI CTAG 1 cut(s) 731
BfuI GTATCC 2 cut(s) 161, 905
BisI GCNGC 4 cut(s) 747, 750, 1105, 1108
BlsI GCNGC 4 cut(s) 748, 751, 1106, 1109
Bme1390I CCNGG 2 cut(s) 562, 563
Bme18I GGWCC 1 cut(s) 662
BmeRI GACNNNNNGTC 2 cut(s) 239, 985
BmeT110I CYCGRG 3 cut(s) 420, 428, 561
BmgT120I GGNCC 2 cut(s) 662, 920
BmiI GGNNCC 2 cut(s) 102, 328
BmrFI CCNGG 2 cut(s) 562, 563
BmsI GCATC 1 cut(s) 1053
BpiI GAAGAC 1 cut(s) 173
BpuMI CCSGG 2 cut(s) 562, 563
Bsa29I ATCGAT 2 cut(s) 246, 507
BsaBI GATNNNNATC 1 cut(s) 509
BsaHI GRCGYC 1 cut(s) 235
BsaI GGTCTC 1 cut(s) 993
BsaJI CCNNGG 1 cut(s) 561
Bse118I RCCGGY 2 cut(s) 106, 215
Bse1I ACTGG 1 cut(s) 777
Bse8I GATNNNNATC 1 cut(s) 509
BseCI ATCGAT 2 cut(s) 246, 507
BseDI CCNNGG 1 cut(s) 561
BseGI GGATG 1 cut(s) 98
BseJI GATNNNNATC 1 cut(s) 509
BseMII CTCAG 2 cut(s) 695, 785
BseNI ACTGG 1 cut(s) 777
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 2 cut(s) 736, 758
BseYI CCCAGC 1 cut(s) 1002
Bsh1285I CGRYCG 2 cut(s) 193, 220
BshFI GGCC 5 cut(s) 86, 219, 549, 815, 922
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 2 cut(s) 246, 507
BsiEI CGRYCG 2 cut(s) 193, 220
BsiHKAI GWGCWC 1 cut(s) 427
BsiHKCI CYCGRG 3 cut(s) 420, 428, 561
BsiSI CCGG 5 cut(s) 87, 107, 159, 216, 562
BslFI GGGAC 1 cut(s) 1019
BsmAI GTCTC 2 cut(s) 993, 1019
BsmFI GGGAC 1 cut(s) 1019
BsnI GGCC 5 cut(s) 86, 219, 549, 815, 922
Bso31I GGTCTC 1 cut(s) 993
BsoBI CYCGRG 3 cut(s) 420, 428, 561
Bsp1286I GDGCHC 1 cut(s) 427
Bsp1407I TGTACA 1 cut(s) 498
Bsp143I GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
BspACI CCGC 5 cut(s) 104, 530, 909, 1104, 1107
BspANI GGCC 5 cut(s) 86, 219, 549, 815, 922
BspCNI CTCAG 2 cut(s) 694, 786
BspDI ATCGAT 2 cut(s) 246, 507
BspLI GGNNCC 2 cut(s) 102, 328
BspPI GGATC 6 cut(s) 204, 316, 355, 443, 783, 798
BspT107I GGYRCC 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 993
BsrFI RCCGGY 2 cut(s) 106, 215
BsrGI TGTACA 1 cut(s) 498
BsrI ACTGG 1 cut(s) 777
BssAI RCCGGY 2 cut(s) 106, 215
BssECI CCNNGG 1 cut(s) 561
BssMI GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
BssNI GRCGYC 1 cut(s) 235
Bst4CI ACNGT 3 cut(s) 191, 326, 759
Bst6I CTCTTC 2 cut(s) 21, 363
BstACI GRCGYC 1 cut(s) 235
BstAUI TGTACA 1 cut(s) 498
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 3 cut(s) 292, 681, 794
BstF5I GGATG 1 cut(s) 98
BstKTI GATC 9 cut(s) 212, 311, 350, 451, 507, 600, 778, 793, 856
BstMAI GTCTC 2 cut(s) 993, 1019
BstMBI GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
BstMCI CGRYCG 2 cut(s) 193, 220
BstMWI GCNNNNNNNGC 2 cut(s) 335, 1043
BstNSI RCATGY 1 cut(s) 906
BstSCI CCNGG 2 cut(s) 560, 561
BstV1I GCAGC 2 cut(s) 736, 758
BstV2I GAAGAC 1 cut(s) 173
BstX2I RGATCY 4 cut(s) 209, 347, 448, 790
BstYI RGATCY 4 cut(s) 209, 347, 448, 790
BstZI CGGCCG 1 cut(s) 217
Bsu15I ATCGAT 2 cut(s) 246, 507
BsuI GTATCC 2 cut(s) 161, 905
BsuRI GGCC 5 cut(s) 86, 219, 549, 815, 922
BsuTUI ATCGAT 2 cut(s) 246, 507
BtgZI GCGATG 1 cut(s) 844
BtsCI GGATG 1 cut(s) 98
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 1 cut(s) 435
Cfr10I RCCGGY 2 cut(s) 106, 215
Cfr13I GGNCC 2 cut(s) 662, 920
Cfr9I CCCGGG 1 cut(s) 561
ClaI ATCGAT 2 cut(s) 246, 507
CseI GACGC 1 cut(s) 243
Csp6I GTAC 2 cut(s) 499, 850
CviAII CATG 5 cut(s) 397, 502, 722, 903, 1048
CviQI GTAC 2 cut(s) 499, 850
DdeI CTNAG 3 cut(s) 292, 681, 794
DpnI GATC 9 cut(s) 211, 310, 349, 450, 506, 599, 777, 792, 855
DpnII GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
DriI GACNNNNNGTC 2 cut(s) 239, 985
EaeI YGGCCR 2 cut(s) 84, 217
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 2 cut(s) 21, 363
Eam1105I GACNNNNNGTC 2 cut(s) 239, 985
EarI CTCTTC 2 cut(s) 21, 363
EciI GGCGGA 1 cut(s) 93
EclXI CGGCCG 1 cut(s) 217
Eco147I AGGCCT 1 cut(s) 549
Eco31I GGTCTC 1 cut(s) 993
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 1 cut(s) 662
Eco52I CGGCCG 1 cut(s) 217
Eco88I CYCGRG 3 cut(s) 420, 428, 561
EcoO109I RGGNCCY 1 cut(s) 920
EcoRV GATATC 1 cut(s) 511
FaeI CATG 5 cut(s) 400, 505, 725, 906, 1051
FaqI GGGAC 1 cut(s) 1019
FatI CATG 5 cut(s) 396, 501, 721, 902, 1047
FauNDI CATATG 1 cut(s) 780
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 4 cut(s) 747, 750, 1105, 1108
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 4 cut(s) 747, 750, 1105, 1108
FspBI CTAG 1 cut(s) 731
GluI GCNGC 4 cut(s) 747, 750, 1105, 1108
GsaI CCCAGC 1 cut(s) 1006
HaeIII GGCC 5 cut(s) 86, 219, 549, 815, 922
HapII CCGG 5 cut(s) 87, 107, 159, 216, 562
HgaI GACGC 1 cut(s) 243
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 5 cut(s) 400, 505, 725, 906, 1051
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 6 cut(s) 128, 150, 162, 296, 483, 533
HpaII CCGG 5 cut(s) 87, 107, 159, 216, 562
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 4 cut(s) 149, 652, 832, 943
Hpy188III TCNNGA 3 cut(s) 205, 420, 788
Hpy8I GTNNAC 4 cut(s) 149, 652, 832, 943
Hpy99I CGWCG 3 cut(s) 158, 233, 983
HpyAV CCTTC 5 cut(s) 44, 56, 623, 761, 977
HpyCH4III ACNGT 3 cut(s) 191, 326, 759
HpyCH4IV ACGT 2 cut(s) 585, 950
HpyCH4V TGCA 3 cut(s) 400, 752, 1066
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 1043
HpyF3I CTNAG 3 cut(s) 292, 681, 794
HpySE526I ACGT 2 cut(s) 585, 950
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 5 cut(s) 400, 505, 725, 906, 1051
Kzo9I GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
Lsp1109I GCAGC 2 cut(s) 736, 758
LweI GCATC 1 cut(s) 1053
MaeI CTAG 1 cut(s) 731
MaeII ACGT 2 cut(s) 585, 950
MalI GATC 9 cut(s) 211, 310, 349, 450, 506, 599, 777, 792, 855
MboI GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
MboII GAAGA 5 cut(s) 8, 173, 342, 350, 1090
MfeI CAATTG 1 cut(s) 339
MflI RGATCY 4 cut(s) 209, 347, 448, 790
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 4 cut(s) 331, 339, 641, 874
MlyI GAGTC 3 cut(s) 144, 156, 290
MmeI TCCRAC 7 cut(s) 91, 156, 162, 252, 286, 325, 894
MseI TTAA 3 cut(s) 117, 762, 896
MspA1I CMGCKG 1 cut(s) 749
MspI CCGG 5 cut(s) 87, 107, 159, 216, 562
MspR9I CCNGG 2 cut(s) 562, 563
MunI CAATTG 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 335, 1043
NciI CCSGG 2 cut(s) 562, 563
NdeI CATATG 1 cut(s) 780
NdeII GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
NlaIII CATG 5 cut(s) 400, 505, 725, 906, 1051
NlaIV GGNNCC 2 cut(s) 102, 328
NspI RCATGY 1 cut(s) 906
PaeR7I CTCGAG 1 cut(s) 428
PceI AGGCCT 1 cut(s) 549
PcsI WCGNNNNNNNCGW 2 cut(s) 237, 591
PfeI GAWTC 3 cut(s) 128, 483, 533
PkrI GCNGC 4 cut(s) 748, 751, 1106, 1109
PleI GAGTC 3 cut(s) 144, 156, 290
PpsI GAGTC 3 cut(s) 144, 156, 290
PsiI TTATAA 2 cut(s) 741, 998
Psp1406I AACGTT 1 cut(s) 585
PspFI CCCAGC 1 cut(s) 1002
PspN4I GGNNCC 2 cut(s) 102, 328
PspPI GGNCC 2 cut(s) 662, 920
PspXI VCTCGAGB 1 cut(s) 428
PsuI RGATCY 4 cut(s) 209, 347, 448, 790
PvuII CAGCTG 1 cut(s) 749
RsaI GTAC 2 cut(s) 500, 851
RsaNI GTAC 2 cut(s) 499, 850
SalI GTCGAC 1 cut(s) 830
SaqAI TTAA 3 cut(s) 117, 762, 896
SatI GCNGC 4 cut(s) 747, 750, 1105, 1108
Sau3AI GATC 9 cut(s) 209, 308, 347, 448, 504, 597, 775, 790, 853
Sau96I GGNCC 2 cut(s) 662, 920
SchI GAGTC 3 cut(s) 144, 156, 290
ScrFI CCNGG 2 cut(s) 562, 563
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 1 cut(s) 1053
Sfr274I CTCGAG 1 cut(s) 428
SgrAI CRCCGGYG 1 cut(s) 106
SinI GGWCC 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 428
SmaI CCCGGG 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 4 cut(s) 331, 339, 641, 874
SseBI AGGCCT 1 cut(s) 549
SsiI CCGC 5 cut(s) 104, 530, 909, 1104, 1107
SspMI CTAG 1 cut(s) 731
StuI AGGCCT 1 cut(s) 549
StyD4I CCNGG 2 cut(s) 560, 561
TaaI ACNGT 3 cut(s) 191, 326, 759
TaiI ACGT 2 cut(s) 588, 953
TaqI TCGA 5 cut(s) 246, 429, 507, 831, 1061
TasI AATT 4 cut(s) 331, 339, 641, 874
TatI WGTACW 1 cut(s) 498
TauI GCSGC 2 cut(s) 1107, 1110
TfiI GAWTC 3 cut(s) 128, 483, 533
Tru1I TTAA 3 cut(s) 117, 762, 896
Tru9I TTAA 3 cut(s) 117, 762, 896
TscAI CASTG 1 cut(s) 87
TseI GCWGC 2 cut(s) 746, 749
TspDTI ATGAA 4 cut(s) 525, 642, 689, 1064
TspGWI ACGGA 6 cut(s) 39, 246, 815, 930, 1023, 1077
TspMI CCCGGG 1 cut(s) 561
TspRI CASTG 1 cut(s) 87
VpaK11BI GGWCC 1 cut(s) 662
XapI RAATTY 1 cut(s) 641
XceI RCATGY 1 cut(s) 906
XhoI CTCGAG 1 cut(s) 428
XmaI CCCGGG 1 cut(s) 561
XmiI GTMKAC 1 cut(s) 831
XspI CTAG 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.