RLG00000025189

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0005861
Physical Location & Seq
Reverse (-)
42900641 .. 42904247
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025189

Sequence Viewer

Length: 1305 bp
ATGAAAGCCAGAGAGGAAGAAGAAGACTATGATCCTTTGTTGTTAGAGAATGGAAATCTTGAAAAGAAAGATAGGGAGGGTTTAGGCAATCCAACAGCGCCTTCTCTTAATCATCAAGCATTGCATTTGCCAAAACATAATATAGCCTTTCATGAACAAATCCCTACTGAAGAAAATGAAAACTACAGTGTCACATCTCAACAGAAGAAGCTGCAGCAGCATTGCTTCGCCGTCTCTATGGCTTTCTCTTTCTCCATCTCCGCCATATTGCTTCTCGTTCTCCTCTCATTCTCCGTCACCGGCCAGCAACCCAGCTATAAGAGCCCCATCATCTTCAATTTCGGAGACTCCAACTCCGACACGGGCGGACTTGTCGCTGGACTCGGCTACACCATCAACCCCCCTTACGGCCGCACCTACTTCCGGCGATCCACCGGCCGGATGTCCGACGGACGCCTCATCATCGACTTCCTCTGCCAAAGTTTGAATACAAGCCTATTGGCTCCATATCTAGACTCTCTGTCAGGGTCTAAGTTCACAAATGGGGCGAATTTTGCAATAGTCGGGTCGTCTATCTTCCCAAAACGCATCCCTTTCTCTTTGAGCATCCAAGTCATGCAGTTCCTTCATTTCAAAGCTACTGCAGGCTCAAAAGACTTGATAGATGATGATGGTTTTCGGAAAGCACTTTACATGATTGATATTGGACAAAATGACCTTGCTCAGTTGTTTTCCAAAAATCTCTCGTTCACGCAAATAACAGAGAAAATCCCACCAATTACTACAGAAATCAAGAAAGCCGTTAAGACTATATATGACCAAGGCGGGCGGAATTTTTGGATACACAACACTGGGCCATTGGGTTGTCTCCCTCAAAAACTTTCATCAGTCCAGCAGAGGGACATGGATACATATGGATGCCTCTCGACCTACAATGCGGCTGCAAAATTGTTCAATGTAGGATTGCGTCATACATGCGATGAGATGAGATTTGAATTGAAGGATGTTAAAGTAGTATATGTAGATGTATATGCCATCAAGTATGACCTCATTGCTAACTACTCCAAATACGGTTTCACTAGTCCTCTAATGGCATGCTGTGGAAACGGAGGTCCTCCATACAACTACAACATGAGGCTGACATGCGGCTATCCTGGTTCTCAGGTTTGCGAGGATGGTTCCCAACTCATAAGTTGGGATGGGATTCACTATACTGAAGCTGCAAATACCATTGTAGCTTCAAAAATACTTTCCACGGATTATTCTATACCGCCAATTCCATTTGATTTCTTTGTTCGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

435

Amino Acids

48.32

Weight (kDa)

6.32

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 111 - 416 2.2e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 261, 366, 412, 825, 829, 938, 1146, 1271
AclWI GGATC 2 cut(s) 26, 423
AcoI YGGCCR 3 cut(s) 301, 409, 436
AcsI RAATTY 2 cut(s) 550, 832
AcuI CTGAAG 2 cut(s) 189, 1236
AcyI GRCGYC 1 cut(s) 454
AfiI CCNNNNNNNGG 4 cut(s) 407, 423, 438, 898
AgsI TTSAA 8 cut(s) 62, 337, 487, 634, 955, 995, 1000, 1242
AhdI GACNNNNNGTC 1 cut(s) 520
AhlI ACTAGT 1 cut(s) 1079
AjnI CCWGG 1 cut(s) 1153
AjuI GAANNNNNNNTTGG 4 cut(s) 85, 117, 1058, 1090
AluBI AGCT 5 cut(s) 211, 315, 638, 1220, 1238
AluI AGCT 5 cut(s) 211, 315, 638, 1220, 1238
Alw26I GTCTC 3 cut(s) 238, 339, 872
AlwI GGATC 2 cut(s) 26, 423
AoxI GGCC 4 cut(s) 301, 409, 436, 854
ApeKI GCWGC 5 cut(s) 211, 214, 217, 941, 1220
ApoI RAATTY 2 cut(s) 550, 832
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 854, 1112
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 1112
BanII GRGCYC 1 cut(s) 326
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 5 cut(s) 198, 226, 229, 928, 1207
BccI CCATC 7 cut(s) 263, 335, 401, 665, 1043, 1169, 1193
BceAI ACGGC 3 cut(s) 215, 424, 785
BciT130I CCWGG 1 cut(s) 1155
BciVI GTATCC 2 cut(s) 834, 901
BcoDI GTCTC 3 cut(s) 238, 339, 872
BcuI ACTAGT 1 cut(s) 1079
BfaI CTAG 2 cut(s) 512, 1080
BfmI CTRYAG 4 cut(s) 184, 212, 642, 783
BfoI RGCGCY 1 cut(s) 101
BfuI GTATCC 2 cut(s) 834, 901
BisI GCNGC 8 cut(s) 212, 215, 218, 412, 939, 942, 1147, 1221
BlsI GCNGC 8 cut(s) 213, 216, 219, 413, 940, 943, 1148, 1222
Bme1390I CCNGG 1 cut(s) 1155
Bme18I GGWCC 1 cut(s) 1112
BmeRI GACNNNNNGTC 1 cut(s) 520
BmgT120I GGNCC 2 cut(s) 854, 1112
BmiI GGNNCC 2 cut(s) 504, 1180
BmrFI CCNGG 1 cut(s) 1155
BmrI ACTGGG 1 cut(s) 861
BmsI GCATC 3 cut(s) 597, 615, 908
BmuI ACTGGG 1 cut(s) 861
BpiI GAAGAC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 454
BsaJI CCNNGG 2 cut(s) 820, 1254
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 423, 438, 898
Bse118I RCCGGY 2 cut(s) 299, 434
Bse1I ACTGG 1 cut(s) 856
Bse3DI GCAATG 3 cut(s) 119, 220, 1050
BseBI CCWGG 1 cut(s) 1155
BseDI CCNNGG 2 cut(s) 820, 1254
BseGI GGATG 7 cut(s) 447, 588, 606, 923, 1009, 1180, 1204
BseLI CCNNNNNNNGG 4 cut(s) 407, 423, 438, 898
BseMI GCAATG 3 cut(s) 119, 220, 1050
BseMII CTCAG 2 cut(s) 737, 1175
BseNI ACTGG 1 cut(s) 856
BseRI GAGGAG 1 cut(s) 272
BseX3I CGGCCG 2 cut(s) 409, 436
BseXI GCAGC 5 cut(s) 198, 226, 229, 928, 1207
BseYI CCCAGC 1 cut(s) 311
Bsh1285I CGRYCG 2 cut(s) 412, 439
BshFI GGCC 4 cut(s) 303, 411, 438, 856
BsiEI CGRYCG 2 cut(s) 412, 439
BsiSI CCGG 4 cut(s) 300, 424, 435, 439
BslFI GGGAC 1 cut(s) 914
BslI CCNNNNNNNGG 4 cut(s) 407, 423, 438, 898
BsmAI GTCTC 3 cut(s) 238, 339, 872
BsmBI CGTCTC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 914
BsnI GGCC 4 cut(s) 303, 411, 438, 856
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 2 cut(s) 31, 428
BspACI CCGC 8 cut(s) 261, 366, 412, 825, 829, 938, 1146, 1271
BspANI GGCC 4 cut(s) 303, 411, 438, 856
BspCNI CTCAG 2 cut(s) 736, 1174
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 504, 1180
BspMAI CTGCAG 2 cut(s) 216, 646
BspPI GGATC 2 cut(s) 26, 423
BsrDI GCAATG 3 cut(s) 119, 220, 1050
BsrFI RCCGGY 2 cut(s) 299, 434
BsrI ACTGG 1 cut(s) 856
BssAI RCCGGY 2 cut(s) 299, 434
BssECI CCNNGG 2 cut(s) 820, 1254
BssMI GATC 2 cut(s) 31, 428
BssNI GRCGYC 1 cut(s) 454
BssT1I CCWWGG 1 cut(s) 820
Bst2UI CCWGG 1 cut(s) 1155
Bst4CI ACNGT 2 cut(s) 188, 1073
BstACI GRCGYC 1 cut(s) 454
BstC8I GCNNGC 4 cut(s) 305, 646, 827, 1096
BstDEI CTNAG 3 cut(s) 531, 723, 1161
BstDSI CCRYGG 1 cut(s) 1254
BstF5I GGATG 7 cut(s) 447, 588, 606, 923, 1009, 1180, 1204
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 2 cut(s) 34, 431
BstMAI GTCTC 3 cut(s) 238, 339, 872
BstMBI GATC 2 cut(s) 31, 428
BstMCI CGRYCG 2 cut(s) 412, 439
BstMWI GCNNNNNNNGC 3 cut(s) 217, 321, 554
BstNI CCWGG 1 cut(s) 1155
BstNSI RCATGY 3 cut(s) 978, 1098, 1146
BstSCI CCNGG 1 cut(s) 1153
BstSFI CTRYAG 4 cut(s) 184, 212, 642, 783
BstV1I GCAGC 5 cut(s) 198, 226, 229, 928, 1207
BstV2I GAAGAC 1 cut(s) 30
BstZI CGGCCG 2 cut(s) 409, 436
BsuI GTATCC 2 cut(s) 834, 901
BsuRI GGCC 4 cut(s) 303, 411, 438, 856
BtgI CCRYGG 1 cut(s) 1254
BtgZI GCGATG 1 cut(s) 993
BtsCI GGATG 7 cut(s) 447, 588, 606, 923, 1009, 1180, 1204
BtsIMutI CAGTG 2 cut(s) 193, 849
Cac8I GCNNGC 4 cut(s) 305, 646, 827, 1096
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 2 cut(s) 299, 434
Cfr13I GGNCC 2 cut(s) 854, 1112
CseI GACGC 2 cut(s) 462, 956
CviAII CATG 8 cut(s) 152, 616, 694, 904, 975, 1095, 1132, 1143
DdeI CTNAG 3 cut(s) 531, 723, 1161
DpnI GATC 2 cut(s) 33, 430
DpnII GATC 2 cut(s) 31, 428
DriI GACNNNNNGTC 1 cut(s) 520
EaeI YGGCCR 3 cut(s) 301, 409, 436
EagI CGGCCG 2 cut(s) 409, 436
Eam1105I GACNNNNNGTC 1 cut(s) 520
EciI GGCGGA 3 cut(s) 250, 381, 844
EclXI CGGCCG 2 cut(s) 409, 436
Eco130I CCWWGG 1 cut(s) 820
Eco24I GRGCYC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 1112
Eco52I CGGCCG 2 cut(s) 409, 436
Eco57I CTGAAG 2 cut(s) 189, 1236
EcoO109I RGGNCCY 1 cut(s) 1112
EcoRII CCWGG 1 cut(s) 1153
EcoT14I CCWWGG 1 cut(s) 820
EcoT38I GRGCYC 1 cut(s) 326
ErhI CCWWGG 1 cut(s) 820
Esp3I CGTCTC 1 cut(s) 238
FaeI CATG 8 cut(s) 155, 619, 697, 907, 978, 1098, 1135, 1146
FaqI GGGAC 1 cut(s) 914
FatI CATG 8 cut(s) 151, 615, 693, 903, 974, 1094, 1131, 1142
FauI CCCGC 1 cut(s) 818
FauNDI CATATG 1 cut(s) 913
Fnu4HI GCNGC 8 cut(s) 212, 215, 218, 412, 939, 942, 1147, 1221
FokI GGATG 7 cut(s) 454, 575, 593, 930, 1016, 1187, 1211
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 8 cut(s) 212, 215, 218, 412, 939, 942, 1147, 1221
FspBI CTAG 2 cut(s) 512, 1080
GlaI GCGC 1 cut(s) 99
GluI GCNGC 8 cut(s) 212, 215, 218, 412, 939, 942, 1147, 1221
GsaI CCCAGC 1 cut(s) 315
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 4 cut(s) 303, 411, 438, 856
HapII CCGG 4 cut(s) 300, 424, 435, 439
HgaI GACGC 2 cut(s) 462, 956
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 454
Hin1II CATG 8 cut(s) 155, 619, 697, 907, 978, 1098, 1135, 1146
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 4 cut(s) 347, 381, 515, 1204
HpaII CCGG 4 cut(s) 300, 424, 435, 439
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 537, 750
Hpy188I TCNGA 4 cut(s) 344, 358, 448, 681
Hpy188III TCNNGA 5 cut(s) 59, 152, 512, 793, 925
Hpy8I GTNNAC 2 cut(s) 537, 750
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 3 cut(s) 111, 635, 994
HpyCH4III ACNGT 2 cut(s) 188, 1073
HpyCH4V TGCA 7 cut(s) 124, 214, 557, 619, 644, 944, 1223
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 321, 554
HpyF3I CTNAG 3 cut(s) 531, 723, 1161
Hsp92I GRCGYC 1 cut(s) 454
Hsp92II CATG 8 cut(s) 155, 619, 697, 907, 978, 1098, 1135, 1146
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 2 cut(s) 31, 428
LmnI GCTCC 1 cut(s) 508
Lsp1109I GCAGC 5 cut(s) 198, 226, 229, 928, 1207
LweI GCATC 3 cut(s) 597, 615, 908
MaeI CTAG 2 cut(s) 512, 1080
MaeIII GTNAC 2 cut(s) 190, 295
MalI GATC 2 cut(s) 33, 430
MboI GATC 2 cut(s) 31, 428
MboII GAAGA 7 cut(s) 29, 32, 35, 182, 217, 325, 568
MhlI GDGCHC 1 cut(s) 326
MluCI AATT 7 cut(s) 337, 550, 777, 832, 947, 995, 1275
MlyI GAGTC 3 cut(s) 341, 375, 509
MmeI TCCRAC 4 cut(s) 116, 375, 381, 471
MseI TTAA 3 cut(s) 108, 804, 1008
MslI CAYNNNNRTG 1 cut(s) 916
MspI CCGG 4 cut(s) 300, 424, 435, 439
MspR9I CCNGG 1 cut(s) 1155
MvaI CCWGG 1 cut(s) 1155
MwoI GCNNNNNNNGC 3 cut(s) 217, 321, 554
NdeI CATATG 1 cut(s) 913
NdeII GATC 2 cut(s) 31, 428
NlaIII CATG 8 cut(s) 155, 619, 697, 907, 978, 1098, 1135, 1146
NlaIV GGNNCC 2 cut(s) 504, 1180
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 2 cut(s) 190, 295
NspI RCATGY 3 cut(s) 978, 1098, 1146
PaeI GCATGC 1 cut(s) 1098
PagI TCATGA 1 cut(s) 151
PfeI GAWTC 1 cut(s) 1204
PkrI GCNGC 8 cut(s) 213, 216, 219, 413, 940, 943, 1148, 1222
PleI GAGTC 3 cut(s) 341, 375, 509
PpsI GAGTC 3 cut(s) 341, 375, 509
PpuMI RGGWCCY 1 cut(s) 1112
Psp5II RGGWCCY 1 cut(s) 1112
Psp6I CCWGG 1 cut(s) 1153
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 1 cut(s) 1153
PspN4I GGNNCC 2 cut(s) 504, 1180
PspPI GGNCC 2 cut(s) 854, 1112
PspPPI RGGWCCY 1 cut(s) 1112
PstI CTGCAG 2 cut(s) 216, 646
RseI CAYNNNNRTG 1 cut(s) 916
SaqAI TTAA 3 cut(s) 108, 804, 1008
SatI GCNGC 8 cut(s) 212, 215, 218, 412, 939, 942, 1147, 1221
Sau3AI GATC 2 cut(s) 31, 428
Sau96I GGNCC 2 cut(s) 854, 1112
SchI GAGTC 3 cut(s) 341, 375, 509
ScrFI CCNGG 1 cut(s) 1155
SduI GDGCHC 1 cut(s) 326
SfaNI GCATC 3 cut(s) 597, 615, 908
SfcI CTRYAG 4 cut(s) 184, 212, 642, 783
SinI GGWCC 1 cut(s) 1112
SmiMI CAYNNNNRTG 1 cut(s) 916
SpeI ACTAGT 1 cut(s) 1079
SphI GCATGC 1 cut(s) 1098
Sse9I AATT 7 cut(s) 337, 550, 777, 832, 947, 995, 1275
SsiI CCGC 8 cut(s) 261, 366, 412, 825, 829, 938, 1146, 1271
SspMI CTAG 2 cut(s) 512, 1080
StyD4I CCNGG 1 cut(s) 1153
StyI CCWWGG 1 cut(s) 820
TaaI ACNGT 2 cut(s) 188, 1073
TaqI TCGA 2 cut(s) 465, 926
TasI AATT 7 cut(s) 337, 550, 777, 832, 947, 995, 1275
TauI GCSGC 3 cut(s) 414, 941, 1149
TfiI GAWTC 1 cut(s) 1204
Tru1I TTAA 3 cut(s) 108, 804, 1008
Tru9I TTAA 3 cut(s) 108, 804, 1008
TscAI CASTG 2 cut(s) 193, 856
TseFI GTSAC 2 cut(s) 190, 295
TseI GCWGC 5 cut(s) 211, 214, 217, 941, 1220
Tsp45I GTSAC 2 cut(s) 190, 295
TspDTI ATGAA 6 cut(s) 17, 140, 168, 192, 617, 873
TspGWI ACGGA 4 cut(s) 283, 465, 1122, 1271
TspRI CASTG 2 cut(s) 193, 856
VpaK11BI GGWCC 1 cut(s) 1112
XapI RAATTY 2 cut(s) 550, 832
XbaI TCTAGA 1 cut(s) 511
XceI RCATGY 3 cut(s) 978, 1098, 1146
XspI CTAG 2 cut(s) 512, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.