Rw0G012220

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00549
OG0005861
Physical Location & Seq
Forward (+)
5032 .. 7776
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012220.1

Sequence Viewer

Length: 1068 bp
ATGGCTTTCTCTTTCTCCATCTCCGCCATATTGCTCCTCGTTCTCCTCTCATTCTCCGTCACCGGCCAGCAACCCAGCTATAAAGGCCCCATCGTCTTCAACTTCGGAGACTCCAACTCCGACACGGGCGGACTTGTCGCTGGACTCGGCTACACCATCAACCCCCCTTACGGCCGCACCTACTTCCGGCGATCCACTGGCCGGATGTCCGACGGACGCCTCATCATCGACTTCCTCTGCCAAAGTTTGAATACAAGCCTTTTGGCTCCATATCTAGACTCTCTGTCAGGGTCTAAGTTCACAAATGGGGCGAATTTTGCAATAGTCGGGTCGTCTATCTTCCCTAAACGCATCCCTTTCTCTTTGAGCATCCAAGTCATGCAGTTCCTTCATTTCAAAGCTACTGCAGGCTCAAAAGACTTGATAGATGATGAAGGTTTTCGGAAAGCACTTTACATGATCGATATTGGACAAAATGACCTTGCTCAGTTGTTTTCCAAAAATCTCTCATTCACGCAAATAACAGAGAAAATCCCACCAATTACTACAGAAATCAAGAAAGCCGTTAAGACTATATATGACCAAGGCGGGCGGAATTTTTGGATACACAACACTGGGCCATTGGGTTGTCTCCCTCAAAAACTTTCATCTGTCCAGCAGAGGGACATGGATACATATGGATGCCTCTCGACCTACAATGCGGCTGCAAAATTGTTCAATGTAGGATTGCGTCATACATGCGATGAGATGAGATTTGAATTGAAGGATGTTAAAGTAGTATATGTAGATATATATGCCATCAAGTATGACCTCATTGCTAACTACACCAAATACGGTTTCACTAGTCCTCTAATGGCATGCTGTGGAAACGGAGGTCCTCCATACAACTACAACATGAGGCTGACATGCGGCTATCCCGGTTCTCAGGTTTGCGAGGATGGATCCCAACTCATAAGTTGGGATGGGATTCACTATACTGAAGCAGCAAATACCATTGTAGCTTCAAAAATACTTTCCACGGATTATTCTATACCGCCAATTCCATTTGATTTCTTTGTTCGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

39.26

Weight (kDa)

8.3

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 32 - 337 1.3e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 24, 129, 175, 588, 592, 701, 909, 1034
AclWI GGATC 3 cut(s) 186, 936, 949
AcoI YGGCCR 3 cut(s) 64, 172, 199
AcsI RAATTY 2 cut(s) 313, 595
AcuI CTGAAG 1 cut(s) 999
AcyI GRCGYC 1 cut(s) 217
AfiI CCNNNNNNNGG 4 cut(s) 170, 186, 201, 661
AgsI TTSAA 7 cut(s) 100, 250, 397, 718, 758, 763, 1005
AhdI GACNNNNNGTC 1 cut(s) 283
AhlI ACTAGT 1 cut(s) 842
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
AluBI AGCT 3 cut(s) 78, 401, 1001
AluI AGCT 3 cut(s) 78, 401, 1001
Alw26I GTCTC 2 cut(s) 102, 635
AlwI GGATC 3 cut(s) 186, 936, 949
AoxI GGCC 5 cut(s) 64, 85, 172, 199, 617
ApeKI GCWGC 2 cut(s) 704, 983
ApoI RAATTY 2 cut(s) 313, 595
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 3 cut(s) 86, 617, 875
AsuC2I CCSGG 1 cut(s) 918
AsuHPI GGTGA 1 cut(s) 52
AvaII GGWCC 1 cut(s) 875
BamHI GGATCC 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 88
BbvI GCAGC 2 cut(s) 691, 995
BccI CCATC 6 cut(s) 26, 98, 164, 806, 932, 956
BceAI ACGGC 2 cut(s) 187, 548
BciVI GTATCC 2 cut(s) 597, 664
BcnI CCSGG 1 cut(s) 918
BcoDI GTCTC 2 cut(s) 102, 635
BcuI ACTAGT 1 cut(s) 842
BfaI CTAG 2 cut(s) 275, 843
BfmI CTRYAG 2 cut(s) 405, 546
BfuI GTATCC 2 cut(s) 597, 664
BisI GCNGC 5 cut(s) 175, 702, 705, 910, 984
BlsI GCNGC 5 cut(s) 176, 703, 706, 911, 985
Bme1390I CCNGG 1 cut(s) 918
Bme18I GGWCC 1 cut(s) 875
BmeRI GACNNNNNGTC 1 cut(s) 283
BmgT120I GGNCC 3 cut(s) 86, 617, 875
BmiI GGNNCC 3 cut(s) 88, 267, 943
BmrFI CCNGG 1 cut(s) 918
BmrI ACTGGG 1 cut(s) 624
BmsI GCATC 3 cut(s) 360, 378, 671
BmuI ACTGGG 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 918
Bsa29I ATCGAT 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 583, 1017
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 186, 201, 661
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 2 cut(s) 202, 619
Bse3DI GCAATG 1 cut(s) 813
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 2 cut(s) 583, 1017
BseGI GGATG 7 cut(s) 210, 351, 369, 686, 772, 943, 967
BseLI CCNNNNNNNGG 4 cut(s) 170, 186, 201, 661
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 2 cut(s) 500, 938
BseNI ACTGG 2 cut(s) 202, 619
BseRI GAGGAG 2 cut(s) 26, 35
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 2 cut(s) 691, 995
BseYI CCCAGC 1 cut(s) 74
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 5 cut(s) 66, 87, 174, 201, 619
BshVI ATCGAT 1 cut(s) 462
BsiEI CGRYCG 1 cut(s) 175
BsiSI CCGG 4 cut(s) 63, 187, 202, 918
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 4 cut(s) 170, 186, 201, 661
BsmAI GTCTC 2 cut(s) 102, 635
BsmFI GGGAC 1 cut(s) 677
BsnI GGCC 5 cut(s) 66, 87, 174, 201, 619
Bsp143I GATC 3 cut(s) 191, 459, 941
BspACI CCGC 8 cut(s) 24, 129, 175, 588, 592, 701, 909, 1034
BspANI GGCC 5 cut(s) 66, 87, 174, 201, 619
BspCNI CTCAG 2 cut(s) 499, 937
BspDI ATCGAT 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 88, 267, 943
BspMAI CTGCAG 1 cut(s) 409
BspPI GGATC 3 cut(s) 186, 936, 949
BsrDI GCAATG 1 cut(s) 813
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 2 cut(s) 202, 619
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 583, 1017
BssMI GATC 3 cut(s) 191, 459, 941
BssNI GRCGYC 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 583
Bst4CI ACNGT 1 cut(s) 836
BstACI GRCGYC 1 cut(s) 217
BstC8I GCNNGC 4 cut(s) 68, 409, 590, 859
BstDEI CTNAG 3 cut(s) 294, 486, 924
BstDSI CCRYGG 1 cut(s) 1017
BstF5I GGATG 7 cut(s) 210, 351, 369, 686, 772, 943, 967
BstKTI GATC 3 cut(s) 194, 462, 944
BstMAI GTCTC 2 cut(s) 102, 635
BstMBI GATC 3 cut(s) 191, 459, 941
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 2 cut(s) 84, 317
BstNSI RCATGY 3 cut(s) 741, 861, 909
BstSCI CCNGG 1 cut(s) 916
BstSFI CTRYAG 2 cut(s) 405, 546
BstV1I GCAGC 2 cut(s) 691, 995
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 1 cut(s) 941
BstYI RGATCY 1 cut(s) 941
BstZI CGGCCG 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 462
BsuI GTATCC 2 cut(s) 597, 664
BsuRI GGCC 5 cut(s) 66, 87, 174, 201, 619
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 1017
BtgZI GCGATG 1 cut(s) 756
BtsCI GGATG 7 cut(s) 210, 351, 369, 686, 772, 943, 967
BtsIMutI CAGTG 2 cut(s) 195, 612
Cac8I GCNNGC 4 cut(s) 68, 409, 590, 859
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 86, 617, 875
ClaI ATCGAT 1 cut(s) 462
CseI GACGC 2 cut(s) 225, 719
CviAII CATG 7 cut(s) 379, 457, 667, 738, 858, 895, 906
DdeI CTNAG 3 cut(s) 294, 486, 924
DpnI GATC 3 cut(s) 193, 461, 943
DpnII GATC 3 cut(s) 191, 459, 941
DriI GACNNNNNGTC 1 cut(s) 283
EaeI YGGCCR 3 cut(s) 64, 172, 199
EagI CGGCCG 1 cut(s) 172
Eam1105I GACNNNNNGTC 1 cut(s) 283
EciI GGCGGA 3 cut(s) 13, 144, 607
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 583
Eco47I GGWCC 1 cut(s) 875
Eco52I CGGCCG 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 999
EcoO109I RGGNCCY 2 cut(s) 86, 875
EcoT14I CCWWGG 1 cut(s) 583
ErhI CCWWGG 1 cut(s) 583
FaeI CATG 7 cut(s) 382, 460, 670, 741, 861, 898, 909
FaqI GGGAC 1 cut(s) 677
FatI CATG 7 cut(s) 378, 456, 666, 737, 857, 894, 905
FauI CCCGC 1 cut(s) 581
FauNDI CATATG 1 cut(s) 676
Fnu4HI GCNGC 5 cut(s) 175, 702, 705, 910, 984
FokI GGATG 7 cut(s) 217, 338, 356, 693, 779, 950, 974
Fsp4HI GCNGC 5 cut(s) 175, 702, 705, 910, 984
FspBI CTAG 2 cut(s) 275, 843
GluI GCNGC 5 cut(s) 175, 702, 705, 910, 984
GsaI CCCAGC 1 cut(s) 78
HaeIII GGCC 5 cut(s) 66, 87, 174, 201, 619
HapII CCGG 4 cut(s) 63, 187, 202, 918
HgaI GACGC 2 cut(s) 225, 719
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 7 cut(s) 382, 460, 670, 741, 861, 898, 909
HinfI GANTC 4 cut(s) 110, 144, 278, 967
HpaII CCGG 4 cut(s) 63, 187, 202, 918
HphI GGTGA 1 cut(s) 52
Hpy166II GTNNAC 1 cut(s) 300
Hpy188I TCNGA 4 cut(s) 107, 121, 211, 444
Hpy188III TCNNGA 3 cut(s) 275, 556, 688
Hpy8I GTNNAC 1 cut(s) 300
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 398, 428, 757
HpyCH4III ACNGT 1 cut(s) 836
HpyCH4V TGCA 4 cut(s) 320, 382, 407, 707
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 317
HpyF3I CTNAG 3 cut(s) 294, 486, 924
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 7 cut(s) 382, 460, 670, 741, 861, 898, 909
Kzo9I GATC 3 cut(s) 191, 459, 941
LmnI GCTCC 2 cut(s) 39, 271
Lsp1109I GCAGC 2 cut(s) 691, 995
LweI GCATC 3 cut(s) 360, 378, 671
MaeI CTAG 2 cut(s) 275, 843
MaeIII GTNAC 1 cut(s) 58
MalI GATC 3 cut(s) 193, 461, 943
MboI GATC 3 cut(s) 191, 459, 941
MboII GAAGA 2 cut(s) 88, 331
MflI RGATCY 1 cut(s) 941
MluCI AATT 6 cut(s) 313, 540, 595, 710, 758, 1038
MlyI GAGTC 3 cut(s) 104, 138, 272
MmeI TCCRAC 3 cut(s) 138, 144, 234
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 2 cut(s) 567, 771
MslI CAYNNNNRTG 1 cut(s) 679
MspI CCGG 4 cut(s) 63, 187, 202, 918
MspR9I CCNGG 1 cut(s) 918
MwoI GCNNNNNNNGC 2 cut(s) 84, 317
NciI CCSGG 1 cut(s) 918
NdeI CATATG 1 cut(s) 676
NdeII GATC 3 cut(s) 191, 459, 941
NlaIII CATG 7 cut(s) 382, 460, 670, 741, 861, 898, 909
NlaIV GGNNCC 3 cut(s) 88, 267, 943
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 58
NspI RCATGY 3 cut(s) 741, 861, 909
PaeI GCATGC 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 967
PkrI GCNGC 5 cut(s) 176, 703, 706, 911, 985
PleI GAGTC 3 cut(s) 104, 138, 272
PpsI GAGTC 3 cut(s) 104, 138, 272
PpuMI RGGWCCY 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 875
PspFI CCCAGC 1 cut(s) 74
PspN4I GGNNCC 3 cut(s) 88, 267, 943
PspPI GGNCC 3 cut(s) 86, 617, 875
PspPPI RGGWCCY 1 cut(s) 875
PstI CTGCAG 1 cut(s) 409
PsuI RGATCY 1 cut(s) 941
RseI CAYNNNNRTG 1 cut(s) 679
SaqAI TTAA 2 cut(s) 567, 771
SatI GCNGC 5 cut(s) 175, 702, 705, 910, 984
Sau3AI GATC 3 cut(s) 191, 459, 941
Sau96I GGNCC 3 cut(s) 86, 617, 875
SchI GAGTC 3 cut(s) 104, 138, 272
ScrFI CCNGG 1 cut(s) 918
SfaNI GCATC 3 cut(s) 360, 378, 671
SfcI CTRYAG 2 cut(s) 405, 546
SinI GGWCC 1 cut(s) 875
SmiMI CAYNNNNRTG 1 cut(s) 679
SpeI ACTAGT 1 cut(s) 842
SphI GCATGC 1 cut(s) 861
Sse9I AATT 6 cut(s) 313, 540, 595, 710, 758, 1038
SsiI CCGC 8 cut(s) 24, 129, 175, 588, 592, 701, 909, 1034
SspMI CTAG 2 cut(s) 275, 843
StyD4I CCNGG 1 cut(s) 916
StyI CCWWGG 1 cut(s) 583
TaaI ACNGT 1 cut(s) 836
TaqI TCGA 3 cut(s) 228, 462, 689
TasI AATT 6 cut(s) 313, 540, 595, 710, 758, 1038
TauI GCSGC 3 cut(s) 177, 704, 912
TfiI GAWTC 1 cut(s) 967
Tru1I TTAA 2 cut(s) 567, 771
Tru9I TTAA 2 cut(s) 567, 771
TscAI CASTG 2 cut(s) 202, 619
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 2 cut(s) 704, 983
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 3 cut(s) 380, 447, 636
TspGWI ACGGA 4 cut(s) 46, 228, 885, 1034
TspRI CASTG 2 cut(s) 202, 619
VpaK11BI GGWCC 1 cut(s) 875
XapI RAATTY 2 cut(s) 313, 595
XbaI TCTAGA 1 cut(s) 274
XceI RCATGY 3 cut(s) 741, 861, 909
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 2 cut(s) 275, 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.