Rorug03G0023200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005861
Physical Location & Seq
Reverse (-)
1840822 .. 1843433
2612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0023200.1

Sequence Viewer

Length: 1572 bp
ATGTCGACCCAAATGGACCAAACCCCAAACCCCAACTCCATCCCAACCAAGAAACGAACCCCCAGGAAGAGGCTCCGAGCTTGCACCAACCACAGCTATGAGCTCACCACGTTTTGCGAACCGGGTCGCCCGATTAGTCCAACCGGCCCATTCCGGGACAACATTTTCATGTTTCTTCAACAATGTGCGGAGCTCACCGACTTCACAGTTCAAGGCATGAGTGTTTGGTTCACTCTTCTTCGTAGCCGTGAGTGTTTGGTTCCTCTTTTTACTATTGAAGAGAACGTGAACACCTCTAAAACCGAGTTCTGTGATCACTGCTTGTCAACTGATTGGAGTAACCATTTTGTGTCGAAGAGAAAGTACCACATGATAATTCCCAAGGATGATTGTTGGACTAAGCCTCTAGATGATAGTGTCTTGGAAGGCACAACGCATCTGTTACATGGTATGATTCACTGTGATGGGATTGGGCATTTGCTATGCATTAATGGACTTAAAGGGGGTTCTAAGCATCTTTGTGGCAGGGAGATCATGGATCTTTGGGATCGGATTTGCACAAATCTTAGAACCAGGAAAATCACAGTTGAAGATTTCTCAAAGAAGCGGTCCATGGAACTTCGACTGCTTCATGGGATTGCTTATGGACATCCTTGGTTCGGTAGATGGGGATACAGGTTTTGCCATGGAAGCTTTGGGGTTGCAGAAGAAACATATGAAAGAGCACTTGATATTCTCAGCTCAATAGAGCTTGAAAAGATCATCCAAGATTTTAGTGACATGGAAGAGTGTGAAGAGCTTAAGAGAGTCATTCATTGCTACAAACATTGGAGCGAATCAAGCTTGGTTACAATCAAAGATCTTCTCAAATTTATGCTCACTGTCAAGGCTGGCTGTCCTGTGAAAAGAAAAGTCAGAAAACCGAAACCGCCTACTACTACTACTCTTTCATCCCTCCGGATCAAGTCCAAGCTGTTTGCTGATGTTATTTCTCACGACCCCGGCAGACATCCTGCAAGAAGACTAAAGATGGCCGCAGATGCGATTGTGCATGTATTGAGAGAAAATGAAAAGAAAGGCTTTGTGAATGGTGAGATGGCGAGGCAAGATGTAAGAGATGCAGCTCTGTTGCATAGTAGAAGAAGTGGGTTGCCTATCAGAGATATAGGTTTGTTGGATAATGTGCTGAGCAAGCTGGACAATGTGATTATTGGGAATCACATTGTCTGCCGCAAGCTGCATGCGATGCCTGGAATTAAGACTGGAATTAGGAAATATACCGTTCATGATCTTCTCGATGGAATCAAGTCCTTGGAACCTGAGAAAGAGTTACTTCTTCCATCACAAGCTCTAGTTCCTGATGTTTACAGTGATGTTCTTTACTTGTACAAGAATGTACTGTTGGGATATCCAGAATCCAAGTTGGTACAATTGGCTACTCAAGTGGTGTTGAACACCAAGTACTTTGTAAAGGAATATCGATTTGGGGATAAGGATGAGCAAGAGCAGTGCCTTGACATTCCTTTGCCAAGTATGACTGATAGTGCTAATCCTGCTGAATCAGGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

523

Amino Acids

59.76

Weight (kDa)

8.62

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_plant_repro PF25874 336 - 415 5.9e-14 Winged helix-turn-helix domain in plant reproductive proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccIII TCCGGA 1 cut(s) 957
AciI CCGC 5 cut(s) 188, 607, 929, 1035, 1231
AclWI GGATC 3 cut(s) 546, 555, 968
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 1 cut(s) 869
AfaI GTAC 5 cut(s) 365, 1388, 1398, 1428, 1463
AfiI CCNNNNNNNGG 3 cut(s) 69, 154, 659
AflII CTTAAG 1 cut(s) 800
AgsI TTSAA 6 cut(s) 179, 212, 278, 590, 755, 1453
AjnI CCWGG 3 cut(s) 62, 572, 1249
AjuI GAANNNNNNNTTGG 4 cut(s) 1385, 1417, 1467, 1499
Alw21I GWGCWC 3 cut(s) 105, 195, 727
AlwI GGATC 3 cut(s) 546, 555, 968
Aor13HI TCCGGA 1 cut(s) 957
AoxI GGCC 2 cut(s) 145, 1032
ApeKI GCWGC 2 cut(s) 1121, 1237
ApoI RAATTY 1 cut(s) 869
ArsI GACNNNNNNTTYG 2 cut(s) 593, 625
AseI ATTAAT 1 cut(s) 489
AspS9I GGNCC 3 cut(s) 16, 146, 609
AsuC2I CCSGG 3 cut(s) 123, 155, 1002
AsuHPI GGTGA 3 cut(s) 97, 187, 1103
AvaII GGWCC 2 cut(s) 16, 609
BanII GRGCYC 2 cut(s) 105, 195
BarI GAAGNNNNNNTAC 2 cut(s) 347, 379
BbsI GAAGAC 1 cut(s) 1027
Bbv12I GWGCWC 3 cut(s) 105, 195, 727
BbvI GCAGC 2 cut(s) 1133, 1224
BccI CCATC 7 cut(s) 47, 458, 660, 1024, 1090, 1292, 1348
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 3 cut(s) 64, 574, 1251
BciVI GTATCC 1 cut(s) 665
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 3 cut(s) 123, 155, 1002
BfaI CTAG 2 cut(s) 407, 1352
BfrI CTTAAG 1 cut(s) 800
BfuI GTATCC 1 cut(s) 665
BglII AGATCT 1 cut(s) 859
BisI GCNGC 4 cut(s) 1035, 1122, 1231, 1238
BlpI GCTNAGC 1 cut(s) 1187
BlsI GCNGC 4 cut(s) 1036, 1123, 1232, 1239
BmcAI AGTACT 1 cut(s) 1463
Bme1390I CCNGG 6 cut(s) 64, 123, 155, 574, 1002, 1251
Bme18I GGWCC 2 cut(s) 16, 609
BmgT120I GGNCC 3 cut(s) 16, 146, 609
BmiI GGNNCC 3 cut(s) 74, 261, 1317
BmrFI CCNGG 6 cut(s) 64, 123, 155, 574, 1002, 1251
BmsI GCATC 5 cut(s) 445, 523, 1030, 1108, 1236
BpiI GAAGAC 1 cut(s) 1027
Bpu1102I GCTNAGC 1 cut(s) 1187
BpuEI CTTGAG 1 cut(s) 1425
BpuMI CCSGG 3 cut(s) 123, 155, 1002
Bsa29I ATCGAT 1 cut(s) 1480
BsaJI CCNNGG 7 cut(s) 62, 381, 612, 653, 685, 1000, 1311
BsaWI WCCGGW 1 cut(s) 957
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 154, 659
Bse118I RCCGGY 1 cut(s) 143
Bse1I ACTGG 1 cut(s) 1267
Bse3DI GCAATG 1 cut(s) 814
BseAI TCCGGA 1 cut(s) 957
BseBI CCWGG 3 cut(s) 64, 574, 1251
BseCI ATCGAT 1 cut(s) 1480
BseDI CCNNGG 7 cut(s) 62, 381, 612, 653, 685, 1000, 1311
BseGI GGATG 7 cut(s) 39, 391, 649, 762, 950, 1009, 1501
BseLI CCNNNNNNNGG 3 cut(s) 69, 154, 659
BseMI GCAATG 1 cut(s) 814
BseMII CTCAG 3 cut(s) 751, 1178, 1311
BseNI ACTGG 1 cut(s) 1267
BseXI GCAGC 2 cut(s) 1133, 1224
BshFI GGCC 2 cut(s) 147, 1034
BshVI ATCGAT 1 cut(s) 1480
BsiHKAI GWGCWC 3 cut(s) 105, 195, 727
BsiSI CCGG 5 cut(s) 122, 144, 154, 958, 1002
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 3 cut(s) 69, 154, 659
BsmFI GGGAC 1 cut(s) 170
BsnI GGCC 2 cut(s) 147, 1034
Bsp1286I GDGCHC 3 cut(s) 105, 195, 727
Bsp13I TCCGGA 1 cut(s) 957
Bsp1407I TGTACA 1 cut(s) 1386
Bsp143I GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
Bsp1720I GCTNAGC 1 cut(s) 1187
Bsp19I CCATGG 2 cut(s) 612, 685
BspACI CCGC 5 cut(s) 188, 607, 929, 1035, 1231
BspANI GGCC 2 cut(s) 147, 1034
BspCNI CTCAG 3 cut(s) 750, 1179, 1312
BspDI ATCGAT 1 cut(s) 1480
BspEI TCCGGA 1 cut(s) 957
BspHI TCATGA 1 cut(s) 1285
BspLI GGNNCC 3 cut(s) 74, 261, 1317
BspPI GGATC 3 cut(s) 546, 555, 968
BspQI GCTCTTC 1 cut(s) 789
BspTI CTTAAG 1 cut(s) 800
BsrDI GCAATG 1 cut(s) 814
BsrFI RCCGGY 1 cut(s) 143
BsrGI TGTACA 1 cut(s) 1386
BsrI ACTGG 1 cut(s) 1267
BssAI RCCGGY 1 cut(s) 143
BssECI CCNNGG 7 cut(s) 62, 381, 612, 653, 685, 1000, 1311
BssMI GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
BssT1I CCWWGG 5 cut(s) 381, 612, 653, 685, 1311
Bst2UI CCWGG 3 cut(s) 64, 574, 1251
Bst4CI ACNGT 7 cut(s) 208, 461, 586, 883, 1282, 1370, 1401
Bst6I CTCTTC 6 cut(s) 62, 240, 273, 350, 780, 789
BstAFI CTTAAG 1 cut(s) 800
BstAPI GCANNNNNTGC 1 cut(s) 1246
BstAUI TGTACA 1 cut(s) 1386
BstC8I GCNNGC 5 cut(s) 82, 892, 1193, 1235, 1242
BstDEI CTNAG 6 cut(s) 399, 510, 566, 737, 1187, 1320
BstDSI CCRYGG 2 cut(s) 612, 685
BstF5I GGATG 7 cut(s) 39, 391, 649, 762, 950, 1009, 1501
BstKTI GATC 8 cut(s) 316, 534, 541, 550, 762, 862, 963, 1291
BstMBI GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
BstMWI GCNNNNNNNGC 6 cut(s) 690, 840, 1040, 1192, 1246, 1553
BstNI CCWGG 3 cut(s) 64, 574, 1251
BstNSI RCATGY 2 cut(s) 1055, 1244
BstSCI CCNGG 6 cut(s) 62, 121, 153, 572, 1000, 1249
BstV1I GCAGC 2 cut(s) 1133, 1224
BstV2I GAAGAC 1 cut(s) 1027
BstX2I RGATCY 2 cut(s) 538, 859
BstYI RGATCY 2 cut(s) 538, 859
Bsu15I ATCGAT 1 cut(s) 1480
BsuI GTATCC 1 cut(s) 665
BsuRI GGCC 2 cut(s) 147, 1034
BsuTUI ATCGAT 1 cut(s) 1480
BtgI CCRYGG 2 cut(s) 612, 685
BtgZI GCGATG 1 cut(s) 1259
BtsCI GGATG 7 cut(s) 39, 391, 649, 762, 950, 1009, 1501
BtsI GCAGTG 2 cut(s) 316, 1514
BtsIMutI CAGTG 5 cut(s) 316, 457, 879, 1375, 1514
Cac8I GCNNGC 5 cut(s) 82, 892, 1193, 1235, 1242
CciI TCATGA 1 cut(s) 1285
Cfr10I RCCGGY 1 cut(s) 143
Cfr13I GGNCC 3 cut(s) 16, 146, 609
ClaI ATCGAT 1 cut(s) 1480
Csp6I GTAC 5 cut(s) 364, 1387, 1397, 1427, 1462
CviQI GTAC 5 cut(s) 364, 1387, 1397, 1427, 1462
DdeI CTNAG 6 cut(s) 399, 510, 566, 737, 1187, 1320
DpnI GATC 8 cut(s) 315, 533, 540, 549, 761, 861, 962, 1290
DpnII GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 6 cut(s) 62, 240, 273, 350, 780, 789
EarI CTCTTC 6 cut(s) 62, 240, 273, 350, 780, 789
Ecl136II GAGCTC 2 cut(s) 103, 193
Eco130I CCWWGG 5 cut(s) 381, 612, 653, 685, 1311
Eco24I GRGCYC 2 cut(s) 105, 195
Eco32I GATATC 1 cut(s) 1409
Eco47I GGWCC 2 cut(s) 16, 609
Eco53kI GAGCTC 2 cut(s) 103, 193
EcoICRI GAGCTC 2 cut(s) 103, 193
EcoRII CCWGG 3 cut(s) 62, 572, 1249
EcoRV GATATC 1 cut(s) 1409
EcoT14I CCWWGG 5 cut(s) 381, 612, 653, 685, 1311
EcoT22I ATGCAT 1 cut(s) 488
EcoT38I GRGCYC 2 cut(s) 105, 195
ErhI CCWWGG 5 cut(s) 381, 612, 653, 685, 1311
FalI AAGNNNNNCTT 4 cut(s) 1064, 1096, 1317, 1349
FaqI GGGAC 1 cut(s) 170
FauNDI CATATG 1 cut(s) 715
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 4 cut(s) 1035, 1122, 1231, 1238
FokI GGATG 7 cut(s) 26, 398, 636, 749, 937, 996, 1508
FriOI GRGCYC 2 cut(s) 105, 195
Fsp4HI GCNGC 4 cut(s) 1035, 1122, 1231, 1238
FspBI CTAG 2 cut(s) 407, 1352
GluI GCNGC 4 cut(s) 1035, 1122, 1231, 1238
HaeIII GGCC 2 cut(s) 147, 1034
HapII CCGG 5 cut(s) 122, 144, 154, 958, 1002
HincII GTYRAC 2 cut(s) 6, 327
HindII GTYRAC 2 cut(s) 6, 327
HindIII AAGCTT 2 cut(s) 691, 841
HinfI GANTC 8 cut(s) 454, 807, 836, 1216, 1302, 1415, 1559, 1566
HpaII CCGG 5 cut(s) 122, 144, 154, 958, 1002
HphI GGTGA 3 cut(s) 97, 187, 1103
Hpy166II GTNNAC 5 cut(s) 6, 231, 289, 327, 1366
Hpy188I TCNGA 4 cut(s) 77, 552, 917, 1160
Hpy188III TCNNGA 8 cut(s) 407, 958, 995, 1286, 1295, 1358, 1412, 1563
Hpy8I GTNNAC 5 cut(s) 6, 231, 289, 327, 1366
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 7 cut(s) 208, 461, 586, 883, 1282, 1370, 1401
HpyCH4IV ACGT 2 cut(s) 110, 285
HpyCH4V TGCA 9 cut(s) 84, 486, 558, 704, 1016, 1051, 1121, 1132, 1240
HpyF10VI GCNNNNNNNGC 6 cut(s) 690, 840, 1040, 1192, 1246, 1553
HpyF3I CTNAG 6 cut(s) 399, 510, 566, 737, 1187, 1320
HpySE526I ACGT 2 cut(s) 110, 285
Kpn2I TCCGGA 1 cut(s) 957
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
LguI GCTCTTC 1 cut(s) 789
LmnI GCTCC 3 cut(s) 78, 190, 831
Lsp1109I GCAGC 2 cut(s) 1133, 1224
LweI GCATC 5 cut(s) 445, 523, 1030, 1108, 1236
MaeI CTAG 2 cut(s) 407, 1352
MaeII ACGT 2 cut(s) 110, 285
MaeIII GTNAC 5 cut(s) 338, 441, 776, 847, 1329
MalI GATC 8 cut(s) 315, 533, 540, 549, 761, 861, 962, 1290
MboI GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
MfeI CAATTG 1 cut(s) 1430
MflI RGATCY 2 cut(s) 538, 859
MhlI GDGCHC 3 cut(s) 105, 195, 727
MluCI AATT 5 cut(s) 375, 869, 1254, 1266, 1430
MlyI GAGTC 1 cut(s) 816
MmeI TCCRAC 3 cut(s) 164, 374, 1155
MnlI CCTC 6 cut(s) 63, 273, 304, 414, 965, 1095
Mph1103I ATGCAT 1 cut(s) 488
MroI TCCGGA 1 cut(s) 957
MseI TTAA 4 cut(s) 489, 498, 801, 1257
MslI CAYNNNNRTG 4 cut(s) 96, 167, 462, 519
MspCI CTTAAG 1 cut(s) 800
MspI CCGG 5 cut(s) 122, 144, 154, 958, 1002
MspR9I CCNGG 6 cut(s) 64, 123, 155, 574, 1002, 1251
MunI CAATTG 1 cut(s) 1430
MvaI CCWGG 3 cut(s) 64, 574, 1251
MwoI GCNNNNNNNGC 6 cut(s) 690, 840, 1040, 1192, 1246, 1553
NciI CCSGG 3 cut(s) 123, 155, 1002
NcoI CCATGG 2 cut(s) 612, 685
NdeI CATATG 1 cut(s) 715
NdeII GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
NlaIV GGNNCC 3 cut(s) 74, 261, 1317
NmuCI GTSAC 1 cut(s) 776
NsiI ATGCAT 1 cut(s) 488
NspI RCATGY 2 cut(s) 1055, 1244
PaeI GCATGC 1 cut(s) 1244
PagI TCATGA 1 cut(s) 1285
PciSI GCTCTTC 1 cut(s) 789
PfeI GAWTC 7 cut(s) 454, 836, 1216, 1302, 1415, 1559, 1566
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 4 cut(s) 1036, 1123, 1232, 1239
PleI GAGTC 1 cut(s) 815
PpsI GAGTC 1 cut(s) 815
PshBI ATTAAT 1 cut(s) 489
Psp124BI GAGCTC 2 cut(s) 105, 195
Psp6I CCWGG 3 cut(s) 62, 572, 1249
PspGI CCWGG 3 cut(s) 62, 572, 1249
PspN4I GGNNCC 3 cut(s) 74, 261, 1317
PspPI GGNCC 3 cut(s) 16, 146, 609
PsrI GAACNNNNNNTAC 2 cut(s) 1445, 1477
PsuI RGATCY 2 cut(s) 538, 859
RsaI GTAC 5 cut(s) 365, 1388, 1398, 1428, 1463
RsaNI GTAC 5 cut(s) 364, 1387, 1397, 1427, 1462
RseI CAYNNNNRTG 4 cut(s) 96, 167, 462, 519
SacI GAGCTC 2 cut(s) 105, 195
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 789
SaqAI TTAA 4 cut(s) 489, 498, 801, 1257
SatI GCNGC 4 cut(s) 1035, 1122, 1231, 1238
Sau3AI GATC 8 cut(s) 313, 531, 538, 547, 759, 859, 960, 1288
Sau96I GGNCC 3 cut(s) 16, 146, 609
ScaI AGTACT 1 cut(s) 1463
SchI GAGTC 1 cut(s) 816
ScrFI CCNGG 6 cut(s) 64, 123, 155, 574, 1002, 1251
SduI GDGCHC 3 cut(s) 105, 195, 727
SfaNI GCATC 5 cut(s) 445, 523, 1030, 1108, 1236
SinI GGWCC 2 cut(s) 16, 609
SmiMI CAYNNNNRTG 4 cut(s) 96, 167, 462, 519
SmlI CTYRAG 2 cut(s) 800, 1440
SmoI CTYRAG 2 cut(s) 800, 1440
SphI GCATGC 1 cut(s) 1244
Sse9I AATT 5 cut(s) 375, 869, 1254, 1266, 1430
SsiI CCGC 5 cut(s) 188, 607, 929, 1035, 1231
SspMI CTAG 2 cut(s) 407, 1352
SstI GAGCTC 2 cut(s) 105, 195
StyD4I CCNGG 6 cut(s) 62, 121, 153, 572, 1000, 1249
StyI CCWWGG 5 cut(s) 381, 612, 653, 685, 1311
TaaI ACNGT 7 cut(s) 208, 461, 586, 883, 1282, 1370, 1401
TaiI ACGT 2 cut(s) 113, 288
TaqI TCGA 5 cut(s) 5, 353, 622, 1296, 1480
TasI AATT 5 cut(s) 375, 869, 1254, 1266, 1430
TatI WGTACW 3 cut(s) 1386, 1396, 1461
TauI GCSGC 2 cut(s) 1037, 1233
TfiI GAWTC 7 cut(s) 454, 836, 1216, 1302, 1415, 1559, 1566
Tru1I TTAA 4 cut(s) 489, 498, 801, 1257
Tru9I TTAA 4 cut(s) 489, 498, 801, 1257
TscAI CASTG 5 cut(s) 323, 464, 886, 1375, 1514
TseFI GTSAC 1 cut(s) 776
TseI GCWGC 2 cut(s) 1121, 1237
Tsp45I GTSAC 1 cut(s) 776
TspDTI ATGAA 7 cut(s) 157, 620, 732, 803, 939, 1083, 1274
TspRI CASTG 5 cut(s) 323, 464, 886, 1375, 1514
Vha464I CTTAAG 1 cut(s) 800
VpaK11BI GGWCC 2 cut(s) 16, 609
VspI ATTAAT 1 cut(s) 489
XapI RAATTY 1 cut(s) 869
XbaI TCTAGA 1 cut(s) 406
XceI RCATGY 2 cut(s) 1055, 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 692
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 407, 1352
ZrmI AGTACT 1 cut(s) 1463
Zsp2I ATGCAT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.