Rw3G007180

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005861
Physical Location & Seq
Forward (+)
6635308 .. 6639645
4338 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007180.1

Sequence Viewer

Length: 996 bp
ATGTATCTAGGTCCAAAGCAGCAGCAGCATTGCTTCGCCGTCTCCATGGCTTTCTCTTTCTCCATCTCCGCCATATTGCTCCTCGTTCTCCTCTCATTCTCCGTCACCGGCCAGCAACCCAGCTATAAAGGCCCCATCGTCTTCAACTTCGGAGACTCCAACTCCGACACGGGCGGACTTGTCGCTGGACTCGGCTACACCATCAACCCCCCTTACGGCCGCACCTACTTCCGGCGATCCACTGGCCGGATGTCCGACGGACGCCTCATCATCGACTTCCTCTGCCAAAGTTTGAATACAAGCCTTTTGGCTCCATATCTAGACTCTCTGTCAGGGTCTAAGTTCACAAATGGGGCGAATTTTGCAATAGTCGGGTCGTCTATCTTCCCTAAACGCATCCCTTTCTCTTTGAGCATCCAAGTCATGCAGTTCCTTCATTTCAAAGCTACTGCAGGCTCAAAAGACTTGATAGATGATGAAGGTTTTCGGAAAGCACTTTACATGATCGATATTGGACAAAATGACCTTGCTCAGTTGCGGGCGGAATTTTTGGATACACAACACTGGGCCATTGGGTTGTCTCCCTCAAAAACTTTCATCGTCCAGCAGAGGGACATGGATACATATGGATGCCTCTCGACCTACAATGCGGCTGCAAAATTGTTCAATGTAGGATTGCGTCATACATGCGATGAGATGAGATTTGAATTGAAGGATGTTAAAGTAGTATATGGAATCAGGATACAATTGTTTTTGTCCCATGTAGGTTTCACTAGTCCTCTAATGGCATGCTGTGGAAACGGAGGTCCTCCATACAACTACAACATGAGGCTGACATGCGGCTATCCCGGTTCTCAGGTTTGCGAGGATGGATCCCAACTTAAGTTGGGATGGGATTCACTAACTGAAGCAGCAAATACCATTGTAGCTTCAAAAATACTTTCCACGGATTATTCTATACCGCCAATTCCATTTGATTTCTTTGTTCGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

331

Amino Acids

36.49

Weight (kDa)

8.06

Isoelectric Point (pI)

29.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 311 1.6e-21 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 69, 174, 220, 538, 542, 650, 840, 962
AclWI GGATC 3 cut(s) 231, 867, 880
AcoI YGGCCR 3 cut(s) 109, 217, 244
AcsI RAATTY 2 cut(s) 358, 545
AcuI CTGAAG 1 cut(s) 927
AcyI GRCGYC 1 cut(s) 262
AfiI CCNNNNNNNGG 4 cut(s) 215, 231, 246, 610
AflII CTTAAG 1 cut(s) 881
AgsI TTSAA 7 cut(s) 145, 295, 442, 667, 707, 712, 933
AhdI GACNNNNNGTC 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 773
AluBI AGCT 3 cut(s) 123, 446, 929
AluI AGCT 3 cut(s) 123, 446, 929
Alw26I GTCTC 3 cut(s) 46, 147, 585
AlwI GGATC 3 cut(s) 231, 867, 880
AoxI GGCC 5 cut(s) 109, 130, 217, 244, 567
ApeKI GCWGC 5 cut(s) 19, 22, 25, 653, 911
ApoI RAATTY 2 cut(s) 358, 545
Asp700I GAANNNNTTC 1 cut(s) 483
AspS9I GGNCC 4 cut(s) 11, 131, 567, 806
AsuC2I CCSGG 1 cut(s) 849
AsuHPI GGTGA 1 cut(s) 97
AvaII GGWCC 2 cut(s) 11, 806
BaeI ACNNNNGTAYC 2 cut(s) 734, 767
BamHI GGATCC 1 cut(s) 872
BbsI GAAGAC 1 cut(s) 133
BbvI GCAGC 5 cut(s) 31, 34, 37, 640, 923
BccI CCATC 5 cut(s) 71, 143, 209, 863, 885
BceAI ACGGC 2 cut(s) 23, 232
BciVI GTATCC 3 cut(s) 547, 613, 735
BcnI CCSGG 1 cut(s) 849
BcoDI GTCTC 3 cut(s) 46, 147, 585
BcuI ACTAGT 1 cut(s) 773
BfaI CTAG 3 cut(s) 8, 320, 774
BfmI CTRYAG 1 cut(s) 450
BfrI CTTAAG 1 cut(s) 881
BfuI GTATCC 3 cut(s) 547, 613, 735
BisI GCNGC 8 cut(s) 20, 23, 26, 220, 651, 654, 841, 912
BlsI GCNGC 8 cut(s) 21, 24, 27, 221, 652, 655, 842, 913
Bme1390I CCNGG 1 cut(s) 849
Bme18I GGWCC 2 cut(s) 11, 806
BmeRI GACNNNNNGTC 1 cut(s) 328
BmgT120I GGNCC 4 cut(s) 11, 131, 567, 806
BmiI GGNNCC 3 cut(s) 133, 312, 874
BmrFI CCNGG 1 cut(s) 849
BmrI ACTGGG 1 cut(s) 574
BmsI GCATC 3 cut(s) 405, 423, 620
BmuI ACTGGG 1 cut(s) 574
BpiI GAAGAC 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 849
Bsa29I ATCGAT 1 cut(s) 507
BsaHI GRCGYC 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 45, 945
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 231, 246, 610
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 2 cut(s) 247, 569
Bse3DI GCAATG 1 cut(s) 28
BseCI ATCGAT 1 cut(s) 507
BseDI CCNNGG 2 cut(s) 45, 945
BseGI GGATG 7 cut(s) 255, 396, 414, 635, 721, 874, 896
BseLI CCNNNNNNNGG 4 cut(s) 215, 231, 246, 610
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 2 cut(s) 545, 869
BseNI ACTGG 2 cut(s) 247, 569
BseRI GAGGAG 2 cut(s) 71, 80
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 5 cut(s) 31, 34, 37, 640, 923
BseYI CCCAGC 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 5 cut(s) 111, 132, 219, 246, 569
BshVI ATCGAT 1 cut(s) 507
BsiEI CGRYCG 1 cut(s) 220
BsiSI CCGG 4 cut(s) 108, 232, 247, 849
BslFI GGGAC 2 cut(s) 626, 742
BslI CCNNNNNNNGG 4 cut(s) 215, 231, 246, 610
BsmAI GTCTC 3 cut(s) 46, 147, 585
BsmBI CGTCTC 1 cut(s) 46
BsmFI GGGAC 2 cut(s) 626, 742
BsnI GGCC 5 cut(s) 111, 132, 219, 246, 569
Bsp143I GATC 3 cut(s) 236, 504, 872
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 8 cut(s) 69, 174, 220, 538, 542, 650, 840, 962
BspANI GGCC 5 cut(s) 111, 132, 219, 246, 569
BspCNI CTCAG 2 cut(s) 544, 868
BspDI ATCGAT 1 cut(s) 507
BspLI GGNNCC 3 cut(s) 133, 312, 874
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 3 cut(s) 231, 867, 880
BspTI CTTAAG 1 cut(s) 881
BsrDI GCAATG 1 cut(s) 28
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 2 cut(s) 247, 569
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 2 cut(s) 45, 945
BssMI GATC 3 cut(s) 236, 504, 872
BssNI GRCGYC 1 cut(s) 262
BssT1I CCWWGG 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 262
BstAFI CTTAAG 1 cut(s) 881
BstC8I GCNNGC 4 cut(s) 113, 454, 540, 790
BstDEI CTNAG 3 cut(s) 339, 531, 855
BstDSI CCRYGG 2 cut(s) 45, 945
BstF5I GGATG 7 cut(s) 255, 396, 414, 635, 721, 874, 896
BstKTI GATC 3 cut(s) 239, 507, 875
BstMAI GTCTC 3 cut(s) 46, 147, 585
BstMBI GATC 3 cut(s) 236, 504, 872
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 3 cut(s) 25, 129, 362
BstNSI RCATGY 3 cut(s) 690, 792, 840
BstSCI CCNGG 1 cut(s) 847
BstSFI CTRYAG 1 cut(s) 450
BstV1I GCAGC 5 cut(s) 31, 34, 37, 640, 923
BstV2I GAAGAC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 872
BstYI RGATCY 1 cut(s) 872
BstZI CGGCCG 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 507
BsuI GTATCC 3 cut(s) 547, 613, 735
BsuRI GGCC 5 cut(s) 111, 132, 219, 246, 569
BsuTUI ATCGAT 1 cut(s) 507
BtgI CCRYGG 2 cut(s) 45, 945
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 7 cut(s) 255, 396, 414, 635, 721, 874, 896
BtsIMutI CAGTG 2 cut(s) 240, 562
Cac8I GCNNGC 4 cut(s) 113, 454, 540, 790
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 4 cut(s) 11, 131, 567, 806
ClaI ATCGAT 1 cut(s) 507
CseI GACGC 2 cut(s) 270, 668
CviAII CATG 9 cut(s) 46, 424, 502, 616, 687, 761, 789, 826, 837
DdeI CTNAG 3 cut(s) 339, 531, 855
DpnI GATC 3 cut(s) 238, 506, 874
DpnII GATC 3 cut(s) 236, 504, 872
DriI GACNNNNNGTC 1 cut(s) 328
EaeI YGGCCR 3 cut(s) 109, 217, 244
EagI CGGCCG 1 cut(s) 217
Eam1105I GACNNNNNGTC 1 cut(s) 328
EciI GGCGGA 3 cut(s) 58, 189, 557
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 1 cut(s) 45
Eco47I GGWCC 2 cut(s) 11, 806
Eco52I CGGCCG 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 927
EcoO109I RGGNCCY 2 cut(s) 131, 806
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
Esp3I CGTCTC 1 cut(s) 46
FaeI CATG 9 cut(s) 49, 427, 505, 619, 690, 764, 792, 829, 840
FaqI GGGAC 2 cut(s) 626, 742
FatI CATG 9 cut(s) 45, 423, 501, 615, 686, 760, 788, 825, 836
FauI CCCGC 1 cut(s) 531
FauNDI CATATG 1 cut(s) 625
Fnu4HI GCNGC 8 cut(s) 20, 23, 26, 220, 651, 654, 841, 912
FokI GGATG 7 cut(s) 262, 383, 401, 642, 728, 881, 903
Fsp4HI GCNGC 8 cut(s) 20, 23, 26, 220, 651, 654, 841, 912
FspBI CTAG 3 cut(s) 8, 320, 774
GluI GCNGC 8 cut(s) 20, 23, 26, 220, 651, 654, 841, 912
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 5 cut(s) 111, 132, 219, 246, 569
HapII CCGG 4 cut(s) 108, 232, 247, 849
HgaI GACGC 2 cut(s) 270, 668
Hin1I GRCGYC 1 cut(s) 262
Hin1II CATG 9 cut(s) 49, 427, 505, 619, 690, 764, 792, 829, 840
HinfI GANTC 5 cut(s) 155, 189, 323, 735, 896
HpaII CCGG 4 cut(s) 108, 232, 247, 849
HphI GGTGA 1 cut(s) 97
Hpy166II GTNNAC 1 cut(s) 345
Hpy188I TCNGA 4 cut(s) 152, 166, 256, 489
Hpy188III TCNNGA 3 cut(s) 320, 637, 739
Hpy8I GTNNAC 1 cut(s) 345
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 443, 473, 706
HpyCH4V TGCA 4 cut(s) 365, 427, 452, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 129, 362
HpyF3I CTNAG 3 cut(s) 339, 531, 855
Hsp92I GRCGYC 1 cut(s) 262
Hsp92II CATG 9 cut(s) 49, 427, 505, 619, 690, 764, 792, 829, 840
Kzo9I GATC 3 cut(s) 236, 504, 872
LmnI GCTCC 2 cut(s) 84, 316
Lsp1109I GCAGC 5 cut(s) 31, 34, 37, 640, 923
LweI GCATC 3 cut(s) 405, 423, 620
MaeI CTAG 3 cut(s) 8, 320, 774
MaeIII GTNAC 1 cut(s) 103
MalI GATC 3 cut(s) 238, 506, 874
MboI GATC 3 cut(s) 236, 504, 872
MboII GAAGA 2 cut(s) 133, 376
MfeI CAATTG 1 cut(s) 746
MflI RGATCY 1 cut(s) 872
MluCI AATT 6 cut(s) 358, 545, 659, 707, 746, 966
MlyI GAGTC 3 cut(s) 149, 183, 317
MmeI TCCRAC 3 cut(s) 183, 189, 279
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 2 cut(s) 720, 882
MslI CAYNNNNRTG 1 cut(s) 628
MspCI CTTAAG 1 cut(s) 881
MspI CCGG 4 cut(s) 108, 232, 247, 849
MspR9I CCNGG 1 cut(s) 849
MunI CAATTG 1 cut(s) 746
MwoI GCNNNNNNNGC 3 cut(s) 25, 129, 362
NciI CCSGG 1 cut(s) 849
NcoI CCATGG 1 cut(s) 45
NdeI CATATG 1 cut(s) 625
NdeII GATC 3 cut(s) 236, 504, 872
NlaIII CATG 9 cut(s) 49, 427, 505, 619, 690, 764, 792, 829, 840
NlaIV GGNNCC 3 cut(s) 133, 312, 874
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 103
NspI RCATGY 3 cut(s) 690, 792, 840
PaeI GCATGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 2 cut(s) 735, 896
PkrI GCNGC 8 cut(s) 21, 24, 27, 221, 652, 655, 842, 913
PleI GAGTC 3 cut(s) 149, 183, 317
PpsI GAGTC 3 cut(s) 149, 183, 317
PpuMI RGGWCCY 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 806
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 3 cut(s) 133, 312, 874
PspPI GGNCC 4 cut(s) 11, 131, 567, 806
PspPPI RGGWCCY 1 cut(s) 806
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 872
RseI CAYNNNNRTG 1 cut(s) 628
SaqAI TTAA 2 cut(s) 720, 882
SatI GCNGC 8 cut(s) 20, 23, 26, 220, 651, 654, 841, 912
Sau3AI GATC 3 cut(s) 236, 504, 872
Sau96I GGNCC 4 cut(s) 11, 131, 567, 806
SchI GAGTC 3 cut(s) 149, 183, 317
ScrFI CCNGG 1 cut(s) 849
SfaNI GCATC 3 cut(s) 405, 423, 620
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 2 cut(s) 11, 806
SmiMI CAYNNNNRTG 1 cut(s) 628
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SpeI ACTAGT 1 cut(s) 773
SphI GCATGC 1 cut(s) 792
Sse9I AATT 6 cut(s) 358, 545, 659, 707, 746, 966
SsiI CCGC 8 cut(s) 69, 174, 220, 538, 542, 650, 840, 962
SspMI CTAG 3 cut(s) 8, 320, 774
StyD4I CCNGG 1 cut(s) 847
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 3 cut(s) 273, 507, 638
TasI AATT 6 cut(s) 358, 545, 659, 707, 746, 966
TauI GCSGC 3 cut(s) 222, 653, 843
TfiI GAWTC 2 cut(s) 735, 896
Tru1I TTAA 2 cut(s) 720, 882
Tru9I TTAA 2 cut(s) 720, 882
TscAI CASTG 2 cut(s) 247, 569
TseFI GTSAC 1 cut(s) 103
TseI GCWGC 5 cut(s) 19, 22, 25, 653, 911
Tsp45I GTSAC 1 cut(s) 103
TspDTI ATGAA 3 cut(s) 425, 492, 586
TspGWI ACGGA 4 cut(s) 91, 273, 816, 962
TspRI CASTG 2 cut(s) 247, 569
Vha464I CTTAAG 1 cut(s) 881
VpaK11BI GGWCC 2 cut(s) 11, 806
XapI RAATTY 2 cut(s) 358, 545
XbaI TCTAGA 1 cut(s) 319
XceI RCATGY 3 cut(s) 690, 792, 840
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 3 cut(s) 8, 320, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.