FvH4_6g08070

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005861
Physical Location & Seq
Forward (+)
4792209 .. 4798685
6477 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g08070.t3

Sequence Viewer

Length: 1308 bp
ATGAAAGCCACAGAGGAAGAAGAAGATTGCGATCCTTTGTTGTTAGAGAGTGGAAGGCTTGAAAAGAAAGATAGGGAGGGTTTAGGCATTCCAATAGTGCCTTCTCCCAATCATCAGGCATTGCATTTGCCAAAACATAATATAGCCTTTCATGAACAAATCCCAACTGAAGAAAATGCAAACTATACAGTCACATCTCAACAGCGACAGCTTCAGCAGTATTGCTTCGCCATATCCATGGCCTTCTCTCTCTCCATCTCCGCCCTATTGCTTCTCTTTCTCCTCTCCTTCTCCGTCACCAGCCTGCAGCAACCTACCAATGGAGGCCCCGTCATCTTCAACTTCGGCGACTCCAACTCCGACACCGGCGGACTCATCGCCGGACTCGGCTACACCATCAACCCCCCTTACGGCCGCACCTACTTCCATCGATCCACCGGCCGCATGTCCGACGGTCGCCTCATCATCGACTTCCTCTGCCAAAGTTTGAACACTAGCCTATTGGCTCCTTACCTAGACTCTTTATCAGGGTCTAAGTTCACAAATGGGGCGAACTTTGCAATAGTTGGGTCGTCTACCTTCCCTAAACGCATCCCTTTCTCTTTGAGCATCCAAGTCATGCAGTTCCTTCATTTCAAAGCCACTGCAGGCGCAAAAGACTTGATAGATGAAGAAGGTTTTCGGAAAGCACTCTACATGATTGACATTGGACAAAATGACCTTGCTCAGTTGTTTTCCAAAAATCTGTCATTAACACAAATAACAAAGAAAATCCCACCCATCACTACTGAAATCAAGAACGCTGTTAAGACTTTATATGATCAAGGCGGGCGTAATTTTTGGATACACAACACTGGGCCATTGGGTTGTCTCCCTCAGAAACTTTCTTCAGTCCAGCAGAGGGACATGGATACATATGGATGCCTCTCAACTTACAATGCGGCTGCAAGATTGTTCAATGTAGGATTGCGCCATACATGCGATGAGATGAGGTTTGAGCTGAAGGATGTCAAAGTAGTATATGTAGATGTATATGCCATCAAGTATGACCTCATTGCTAACTCCACCAAATACGGTTTCACTAGTCCTCTAATGGCATGCTGTGGAAACGGAGGTCCTCCATACAACTACAACATGAGGCTGACATGCGGCTATCCCGGTTCTCAGGTTTGCAAGGATACATCCCAATTCATAAGTTGGGATGGGATTCACTACACTGAAGCTGCAAACACCATTGTAGCCTCAAAGATACTATCGACAGATTATTCTATACCGCCGATTCCGTTTGATTTCTTTGTTCGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

436

Amino Acids

48.27

Weight (kDa)

6.51

Isoelectric Point (pI)

36.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 33 - 191 1e-19 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 575
AciI CCGC 8 cut(s) 261, 369, 415, 442, 828, 941, 1149, 1274
AclWI GGATC 2 cut(s) 26, 426
AcoI YGGCCR 2 cut(s) 412, 439
AcuI CTGAAG 5 cut(s) 189, 197, 873, 1022, 1239
AfiI CCNNNNNNNGG 3 cut(s) 320, 410, 901
AgsI TTSAA 5 cut(s) 62, 340, 490, 637, 958
AhlI ACTAGT 1 cut(s) 1082
AjuI GAANNNNNNNTTGG 4 cut(s) 85, 117, 1061, 1093
AluBI AGCT 3 cut(s) 211, 1000, 1223
AluI AGCT 3 cut(s) 211, 1000, 1223
Alw26I GTCTC 1 cut(s) 875
AlwI GGATC 2 cut(s) 26, 426
AoxI GGCC 5 cut(s) 240, 325, 412, 439, 857
ApeKI GCWGC 3 cut(s) 307, 944, 1223
Asp700I GAANNNNTTC 1 cut(s) 678
AspLEI GCGC 2 cut(s) 653, 972
AspS9I GGNCC 3 cut(s) 326, 857, 1115
AsuC2I CCSGG 1 cut(s) 1158
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 1115
BbvI GCAGC 3 cut(s) 319, 931, 1210
BccI CCATC 6 cut(s) 263, 404, 435, 788, 1046, 1196
BceAI ACGGC 1 cut(s) 427
BciVI GTATCC 3 cut(s) 837, 904, 1171
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 1158
BcoDI GTCTC 1 cut(s) 875
BcuI ACTAGT 1 cut(s) 1082
BfaI CTAG 3 cut(s) 495, 515, 1083
BfmI CTRYAG 2 cut(s) 305, 645
BfuI GTATCC 3 cut(s) 837, 904, 1171
BisI GCNGC 7 cut(s) 308, 415, 442, 942, 945, 1150, 1224
BlsI GCNGC 7 cut(s) 309, 416, 443, 943, 946, 1151, 1225
Bme1390I CCNGG 1 cut(s) 1158
Bme18I GGWCC 1 cut(s) 1115
BmgT120I GGNCC 3 cut(s) 326, 857, 1115
BmiI GGNNCC 2 cut(s) 328, 507
BmrFI CCNGG 1 cut(s) 1158
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 3 cut(s) 600, 618, 911
BmuI ACTGGG 1 cut(s) 864
BpuMI CCSGG 1 cut(s) 1158
Bsa29I ATCGAT 1 cut(s) 430
BsaBI GATNNNNATC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 237
BsaXI ACNNNNNCTCC 4 cut(s) 315, 341, 345, 371
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 410, 901
Bse118I RCCGGY 2 cut(s) 365, 437
Bse1I ACTGG 1 cut(s) 859
Bse3DI GCAATG 2 cut(s) 119, 1053
Bse8I GATNNNNATC 1 cut(s) 30
BseCI ATCGAT 1 cut(s) 430
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 6 cut(s) 591, 609, 926, 1012, 1181, 1207
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 3 cut(s) 320, 410, 901
BseMI GCAATG 2 cut(s) 119, 1053
BseMII CTCAG 3 cut(s) 740, 890, 1178
BseNI ACTGG 1 cut(s) 859
BseRI GAGGAG 1 cut(s) 272
BseX3I CGGCCG 2 cut(s) 412, 439
BseXI GCAGC 3 cut(s) 319, 931, 1210
Bsh1285I CGRYCG 3 cut(s) 415, 442, 457
BshFI GGCC 5 cut(s) 242, 327, 414, 441, 859
BshVI ATCGAT 1 cut(s) 430
BsiEI CGRYCG 3 cut(s) 415, 442, 457
BsiSI CCGG 4 cut(s) 366, 381, 438, 1158
BslFI GGGAC 1 cut(s) 917
BslI CCNNNNNNNGG 3 cut(s) 320, 410, 901
BsmAI GTCTC 1 cut(s) 875
BsmFI GGGAC 1 cut(s) 917
BsmI GAATGC 1 cut(s) 87
BsnI GGCC 5 cut(s) 242, 327, 414, 441, 859
Bsp143I GATC 3 cut(s) 31, 431, 820
Bsp19I CCATGG 1 cut(s) 237
BspACI CCGC 8 cut(s) 261, 369, 415, 442, 828, 941, 1149, 1274
BspANI GGCC 5 cut(s) 242, 327, 414, 441, 859
BspCNI CTCAG 3 cut(s) 739, 889, 1177
BspDI ATCGAT 1 cut(s) 430
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 328, 507
BspMAI CTGCAG 2 cut(s) 309, 649
BspPI GGATC 2 cut(s) 26, 426
BsrDI GCAATG 2 cut(s) 119, 1053
BsrFI RCCGGY 2 cut(s) 365, 437
BsrI ACTGG 1 cut(s) 859
BssAI RCCGGY 2 cut(s) 365, 437
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 3 cut(s) 31, 431, 820
BssT1I CCWWGG 1 cut(s) 237
Bst4CI ACNGT 3 cut(s) 190, 455, 1076
BstC8I GCNNGC 4 cut(s) 305, 649, 830, 1099
BstDEI CTNAG 4 cut(s) 534, 726, 876, 1164
BstDSI CCRYGG 1 cut(s) 237
BstF5I GGATG 6 cut(s) 591, 609, 926, 1012, 1181, 1207
BstHHI GCGC 2 cut(s) 653, 972
BstKTI GATC 3 cut(s) 34, 434, 823
BstMAI GTCTC 1 cut(s) 875
BstMBI GATC 3 cut(s) 31, 431, 820
BstMCI CGRYCG 3 cut(s) 415, 442, 457
BstMWI GCNNNNNNNGC 2 cut(s) 557, 978
BstNSI RCATGY 4 cut(s) 448, 981, 1101, 1149
BstSCI CCNGG 1 cut(s) 1156
BstSFI CTRYAG 2 cut(s) 305, 645
BstV1I GCAGC 3 cut(s) 319, 931, 1210
BstXI CCANNNNNNTGG 1 cut(s) 238
BstZI CGGCCG 2 cut(s) 412, 439
Bsu15I ATCGAT 1 cut(s) 430
BsuI GTATCC 3 cut(s) 837, 904, 1171
BsuRI GGCC 5 cut(s) 242, 327, 414, 441, 859
BsuTUI ATCGAT 1 cut(s) 430
BtgI CCRYGG 1 cut(s) 237
BtgZI GCGATG 2 cut(s) 361, 996
BtsCI GGATG 6 cut(s) 591, 609, 926, 1012, 1181, 1207
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 3 cut(s) 642, 852, 1215
Cac8I GCNNGC 4 cut(s) 305, 649, 830, 1099
CciI TCATGA 1 cut(s) 151
CfoI GCGC 2 cut(s) 653, 972
Cfr10I RCCGGY 2 cut(s) 365, 437
Cfr13I GGNCC 3 cut(s) 326, 857, 1115
ClaI ATCGAT 1 cut(s) 430
DdeI CTNAG 4 cut(s) 534, 726, 876, 1164
DpnI GATC 3 cut(s) 33, 433, 822
DpnII GATC 3 cut(s) 31, 431, 820
EaeI YGGCCR 2 cut(s) 412, 439
EagI CGGCCG 2 cut(s) 412, 439
EciI GGCGGA 2 cut(s) 250, 384
EclXI CGGCCG 2 cut(s) 412, 439
Eco130I CCWWGG 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 1115
Eco52I CGGCCG 2 cut(s) 412, 439
Eco57I CTGAAG 5 cut(s) 189, 197, 873, 1022, 1239
EcoO109I RGGNCCY 2 cut(s) 326, 1115
EcoT14I CCWWGG 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 237
FaqI GGGAC 1 cut(s) 917
FauI CCCGC 1 cut(s) 821
FauNDI CATATG 1 cut(s) 916
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 1 cut(s) 575
Fnu4HI GCNGC 7 cut(s) 308, 415, 442, 942, 945, 1150, 1224
FokI GGATG 6 cut(s) 578, 596, 933, 1019, 1168, 1214
Fsp4HI GCNGC 7 cut(s) 308, 415, 442, 942, 945, 1150, 1224
FspBI CTAG 3 cut(s) 495, 515, 1083
GlaI GCGC 2 cut(s) 652, 971
GluI GCNGC 7 cut(s) 308, 415, 442, 942, 945, 1150, 1224
HaeIII GGCC 5 cut(s) 242, 327, 414, 441, 859
HapII CCGG 4 cut(s) 366, 381, 438, 1158
HhaI GCGC 2 cut(s) 653, 972
Hin6I GCGC 2 cut(s) 651, 970
HinP1I GCGC 2 cut(s) 651, 970
HinfI GANTC 6 cut(s) 350, 372, 384, 518, 1207, 1279
HpaII CCGG 4 cut(s) 366, 381, 438, 1158
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 2 cut(s) 540, 576
Hpy188I TCNGA 4 cut(s) 361, 451, 684, 879
Hpy188III TCNNGA 2 cut(s) 152, 796
Hpy8I GTNNAC 2 cut(s) 540, 576
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 8 cut(s) 48, 111, 253, 298, 589, 638, 668, 997
HpyCH4III ACNGT 3 cut(s) 190, 455, 1076
HpyCH4V TGCA 9 cut(s) 124, 179, 307, 560, 622, 647, 947, 1173, 1226
HpyF10VI GCNNNNNNNGC 2 cut(s) 557, 978
HpyF3I CTNAG 4 cut(s) 534, 726, 876, 1164
HspAI GCGC 2 cut(s) 651, 970
Ksp22I TGATCA 1 cut(s) 820
Kzo9I GATC 3 cut(s) 31, 431, 820
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 3 cut(s) 319, 931, 1210
LweI GCATC 3 cut(s) 600, 618, 911
MaeI CTAG 3 cut(s) 495, 515, 1083
MaeIII GTNAC 2 cut(s) 190, 295
MalI GATC 3 cut(s) 33, 433, 822
MboI GATC 3 cut(s) 31, 431, 820
MboII GAAGA 7 cut(s) 29, 32, 35, 182, 328, 683, 879
MluCI AATT 2 cut(s) 835, 1187
MlyI GAGTC 4 cut(s) 344, 366, 378, 512
MmeI TCCRAC 3 cut(s) 378, 384, 474
MroXI GAANNNNTTC 1 cut(s) 678
MseI TTAA 2 cut(s) 752, 807
MslI CAYNNNNRTG 2 cut(s) 236, 919
MspI CCGG 4 cut(s) 366, 381, 438, 1158
MspR9I CCNGG 1 cut(s) 1158
Mva1269I GAATGC 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 557, 978
NciI CCSGG 1 cut(s) 1158
NcoI CCATGG 1 cut(s) 237
NdeI CATATG 1 cut(s) 916
NdeII GATC 3 cut(s) 31, 431, 820
NlaIV GGNNCC 2 cut(s) 328, 507
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 2 cut(s) 190, 295
NspI RCATGY 4 cut(s) 448, 981, 1101, 1149
PaeI GCATGC 1 cut(s) 1101
PagI TCATGA 1 cut(s) 151
PctI GAATGC 1 cut(s) 87
PdmI GAANNNNTTC 1 cut(s) 678
PfeI GAWTC 2 cut(s) 1207, 1279
PkrI GCNGC 7 cut(s) 309, 416, 443, 943, 946, 1151, 1225
PleI GAGTC 4 cut(s) 344, 366, 378, 512
PpsI GAGTC 4 cut(s) 344, 366, 378, 512
PpuMI RGGWCCY 1 cut(s) 1115
Psp5II RGGWCCY 1 cut(s) 1115
PspN4I GGNNCC 2 cut(s) 328, 507
PspPI GGNCC 3 cut(s) 326, 857, 1115
PspPPI RGGWCCY 1 cut(s) 1115
PstI CTGCAG 2 cut(s) 309, 649
RseI CAYNNNNRTG 2 cut(s) 236, 919
SaqAI TTAA 2 cut(s) 752, 807
SatI GCNGC 7 cut(s) 308, 415, 442, 942, 945, 1150, 1224
Sau3AI GATC 3 cut(s) 31, 431, 820
Sau96I GGNCC 3 cut(s) 326, 857, 1115
SchI GAGTC 4 cut(s) 344, 366, 378, 512
ScrFI CCNGG 1 cut(s) 1158
SfaNI GCATC 3 cut(s) 600, 618, 911
SfcI CTRYAG 2 cut(s) 305, 645
SgrAI CRCCGGYG 1 cut(s) 365
SinI GGWCC 1 cut(s) 1115
SmiMI CAYNNNNRTG 2 cut(s) 236, 919
SpeI ACTAGT 1 cut(s) 1082
SphI GCATGC 1 cut(s) 1101
Sse9I AATT 2 cut(s) 835, 1187
SsiI CCGC 8 cut(s) 261, 369, 415, 442, 828, 941, 1149, 1274
SspMI CTAG 3 cut(s) 495, 515, 1083
StyD4I CCNGG 1 cut(s) 1156
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 3 cut(s) 190, 455, 1076
TaqI TCGA 3 cut(s) 430, 468, 1256
TasI AATT 2 cut(s) 835, 1187
TauI GCSGC 4 cut(s) 417, 444, 944, 1152
TfiI GAWTC 2 cut(s) 1207, 1279
Tru1I TTAA 2 cut(s) 752, 807
Tru9I TTAA 2 cut(s) 752, 807
TscAI CASTG 3 cut(s) 649, 859, 1222
TseFI GTSAC 2 cut(s) 190, 295
TseI GCWGC 3 cut(s) 307, 944, 1223
Tsp45I GTSAC 2 cut(s) 190, 295
TspDTI ATGAA 6 cut(s) 17, 140, 168, 620, 684, 1180
TspGWI ACGGA 3 cut(s) 283, 1125, 1272
TspRI CASTG 3 cut(s) 649, 859, 1222
VpaK11BI GGWCC 1 cut(s) 1115
XceI RCATGY 4 cut(s) 448, 981, 1101, 1149
XmiI GTMKAC 1 cut(s) 575
XmnI GAANNNNTTC 1 cut(s) 678
XspI CTAG 3 cut(s) 495, 515, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.