MD12G1202600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005861
Physical Location & Seq
Reverse (-)
28293202 .. 28297602
4401 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1202600.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGTCTTGCACTCAGTCCCTGGGTCTCACTCTCACGTTCTTCGGTGCCGTCCTTCTGATTATTCTCTCAGCCTCCGTCGATGGCCAGCGCAATCCGAGGCCCATCATCTTCAACTTTGGGGACTCCAACTCGGACACCGGTGGACTTGTCGCTGGACTTGGCTTCTCCGTCAACCCCCCCTACGGCCGTACTTTCTTCCGCAGATCAACCGGCCGCCTATCCGACGGCCGCCTCGTCCTCGACCTCCTCTGCCAAAGTTTGAATGGAGGTCTGTTGAGTCCATACCTAGACTCTCTGGCAGGGTCAAGGTTCACAAATGGAGCCAACTTTGCAGTTGTCGGGTCCTCTACCCTTCCGAAACGTGTCCCGTTTTCGTTGAATATTCAGGTCATGCAGTTCATCCACTTCAAGGCTACTGCAGGTTCAAAACATTTGATTAGTGATGAAGGTTTCCGGAAAGCACTTTACATGATCGATATTGGACAAAATGATCTTGCTGATTCGTTTGGTAAAAACCTTTCGTACGCTCAAGTGACGAAGAGAATCCCATCGGTTATTGCAGCAATCAAGAACGCCGTTCAGGCTTTATATGATCAAGGTGGGAGGAACTTTTGGATACACAATACCGGGCCATTGGGATGTCTCCCTCAAAAGCTTTCGTTAGTCCAGAAGAAGGATTTGGATCCACATGGATGTCTTTCAAGTTACAACGCGGCCGCGAGGTTGTTCAACGAAGGATTGCGCCATCTATGCAATGGGATGAGACCTCAATTGAAGGATGCTACTATAGTTTATGTAGATGTATACGCCATCAAGTACGATCTCATTGCCAACGCCATGAAATACGGTTTCTCTAGCCCGCTAATGGCATGCTGCGGCAATGGAGGCCCTCCGTACAACTACAACATCGGTTTGACGTGTGGTCATCGTGGTTCTAATGTCTGCGATGAAGGATCCCGATACGTGAGCTGGGATGGAATTCATTTCACCGAGGCTGCAAACACAATAGTAGCCTCGAAAATACTTTCCACAAAATATTCCACGCCAAGCATTCCATTTGATTTCTTTTGTCGCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

38.9

Weight (kDa)

9.11

Isoelectric Point (pI)

36.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 36 - 341 2e-49 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 804
AccII CGCG 3 cut(s) 713, 719, 1074
AccIII TCCGGA 1 cut(s) 453
AciI CCGC 8 cut(s) 199, 214, 229, 713, 717, 860, 876, 1074
AclWI GGATC 4 cut(s) 677, 690, 948, 961
AcoI YGGCCR 5 cut(s) 82, 184, 211, 226, 714
AcsI RAATTY 1 cut(s) 978
AfaI GTAC 4 cut(s) 190, 524, 818, 896
AfiI CCNNNNNNNGG 4 cut(s) 182, 409, 673, 865
AflIII ACRYGT 2 cut(s) 361, 917
AgeI ACCGGT 1 cut(s) 137
AgsI TTSAA 8 cut(s) 112, 262, 379, 409, 426, 702, 730, 775
AhdI GACNNNNNGTC 1 cut(s) 921
AjiI CACGTC 1 cut(s) 918
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 2 cut(s) 662, 694
AluBI AGCT 2 cut(s) 655, 969
AluI AGCT 2 cut(s) 655, 969
Alw26I GTCTC 3 cut(s) 29, 647, 757
AlwI GGATC 4 cut(s) 677, 690, 948, 961
AlwNI CAGNNNCTG 1 cut(s) 19
Aor13HI TCCGGA 1 cut(s) 453
AoxI GGCC 8 cut(s) 82, 98, 184, 211, 226, 629, 714, 886
ApeKI GCWGC 3 cut(s) 560, 873, 995
ApoI RAATTY 1 cut(s) 978
AsiGI ACCGGT 1 cut(s) 137
AspLEI GCGC 2 cut(s) 90, 744
AspS9I GGNCC 4 cut(s) 99, 342, 629, 887
AsuC2I CCSGG 1 cut(s) 628
AsuHPI GGTGA 1 cut(s) 979
AvaII GGWCC 1 cut(s) 342
BalI TGGCCA 1 cut(s) 84
BamHI GGATCC 2 cut(s) 682, 953
BanI GGYRCC 1 cut(s) 44
BbvI GCAGC 3 cut(s) 572, 860, 982
BccI CCATC 6 cut(s) 74, 110, 556, 753, 818, 968
BceAI ACGGC 5 cut(s) 32, 171, 199, 241, 560
BcgI CGANNNNNNTGC 2 cut(s) 809, 843
BciT130I CCWGG 1 cut(s) 20
BciVI GTATCC 1 cut(s) 609
BclI TGATCA 1 cut(s) 592
BcnI CCSGG 1 cut(s) 628
BcoDI GTCTC 3 cut(s) 29, 647, 757
BfaI CTAG 2 cut(s) 287, 855
BfmI CTRYAG 2 cut(s) 417, 786
BfuAI ACCTGC 1 cut(s) 410
BfuI GTATCC 1 cut(s) 609
BglI GCCNNNNNGGC 1 cut(s) 581
BisI GCNGC 8 cut(s) 214, 229, 561, 714, 717, 874, 877, 996
BlsI GCNGC 8 cut(s) 215, 230, 562, 715, 718, 875, 878, 997
Bme1390I CCNGG 2 cut(s) 20, 628
Bme18I GGWCC 1 cut(s) 342
BmeRI GACNNNNNGTC 1 cut(s) 921
BmgBI CACGTC 1 cut(s) 918
BmgT120I GGNCC 4 cut(s) 99, 342, 629, 887
BmiI GGNNCC 5 cut(s) 46, 322, 343, 684, 955
BmrFI CCNGG 2 cut(s) 20, 628
BmsI GCATC 1 cut(s) 769
BpuEI CTTGAG 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 628
Bsa29I ATCGAT 1 cut(s) 474
BsaAI YACGTR 1 cut(s) 964
BsaBI GATNNNNATC 1 cut(s) 681
BsaI GGTCTC 2 cut(s) 29, 757
BsaJI CCNNGG 4 cut(s) 18, 19, 95, 990
BsaWI WCCGGW 2 cut(s) 137, 453
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 409, 673, 865
Bse118I RCCGGY 2 cut(s) 137, 209
Bse3DI GCAATG 3 cut(s) 760, 825, 886
Bse8I GATNNNNATC 1 cut(s) 681
BseAI TCCGGA 1 cut(s) 453
BseBI CCWGG 1 cut(s) 20
BseCI ATCGAT 1 cut(s) 474
BseDI CCNNGG 4 cut(s) 18, 19, 95, 990
BseGI GGATG 6 cut(s) 399, 644, 698, 765, 784, 979
BseJI GATNNNNATC 1 cut(s) 681
BseLI CCNNNNNNNGG 4 cut(s) 182, 409, 673, 865
BseMI GCAATG 3 cut(s) 760, 825, 886
BseMII CTCAG 2 cut(s) 26, 81
BseRI GAGGAG 1 cut(s) 236
BseX3I CGGCCG 4 cut(s) 184, 211, 226, 714
BseXI GCAGC 3 cut(s) 572, 860, 982
BseYI CCCAGC 1 cut(s) 969
Bsh1236I CGCG 3 cut(s) 713, 719, 1074
Bsh1285I CGRYCG 4 cut(s) 187, 214, 229, 717
BshFI GGCC 8 cut(s) 84, 100, 186, 213, 228, 631, 716, 888
BshNI GGYRCC 1 cut(s) 44
BshTI ACCGGT 1 cut(s) 137
BshVI ATCGAT 1 cut(s) 474
BsiEI CGRYCG 4 cut(s) 187, 214, 229, 717
BsiSI CCGG 4 cut(s) 138, 210, 454, 627
BsiWI CGTACG 1 cut(s) 522
BslFI GGGAC 2 cut(s) 134, 350
BslI CCNNNNNNNGG 4 cut(s) 182, 409, 673, 865
BsmAI GTCTC 3 cut(s) 29, 647, 757
BsmFI GGGAC 2 cut(s) 134, 350
BsmI GAATGC 1 cut(s) 1050
BsnI GGCC 8 cut(s) 84, 100, 186, 213, 228, 631, 716, 888
Bso31I GGTCTC 2 cut(s) 29, 757
Bsp13I TCCGGA 1 cut(s) 453
Bsp143I GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
BspACI CCGC 8 cut(s) 199, 214, 229, 713, 717, 860, 876, 1074
BspANI GGCC 8 cut(s) 84, 100, 186, 213, 228, 631, 716, 888
BspCNI CTCAG 2 cut(s) 25, 80
BspDI ATCGAT 1 cut(s) 474
BspEI TCCGGA 1 cut(s) 453
BspFNI CGCG 3 cut(s) 713, 719, 1074
BspLI GGNNCC 5 cut(s) 46, 322, 343, 684, 955
BspMAI CTGCAG 1 cut(s) 421
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 4 cut(s) 677, 690, 948, 961
BspT107I GGYRCC 1 cut(s) 44
BspTNI GGTCTC 2 cut(s) 29, 757
BsrDI GCAATG 3 cut(s) 760, 825, 886
BsrFI RCCGGY 2 cut(s) 137, 209
BssAI RCCGGY 2 cut(s) 137, 209
BssECI CCNNGG 4 cut(s) 18, 19, 95, 990
BssMI GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
BssNAI GTATAC 1 cut(s) 805
Bst1107I GTATAC 1 cut(s) 805
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 848
Bst6I CTCTTC 1 cut(s) 533
BstBAI YACGTR 1 cut(s) 964
BstC8I GCNNGC 3 cut(s) 86, 860, 871
BstDEI CTNAG 2 cut(s) 12, 67
BstF5I GGATG 6 cut(s) 399, 644, 698, 765, 784, 979
BstFNI CGCG 3 cut(s) 713, 719, 1074
BstHHI GCGC 2 cut(s) 90, 744
BstKTI GATC 7 cut(s) 206, 474, 493, 595, 685, 823, 956
BstMAI GTCTC 3 cut(s) 29, 647, 757
BstMBI GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
BstMCI CGRYCG 4 cut(s) 187, 214, 229, 717
BstMWI GCNNNNNNNGC 4 cut(s) 329, 581, 750, 885
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 1 cut(s) 873
BstSCI CCNGG 2 cut(s) 18, 626
BstSFI CTRYAG 2 cut(s) 417, 786
BstUI CGCG 3 cut(s) 713, 719, 1074
BstV1I GCAGC 3 cut(s) 572, 860, 982
BstX2I RGATCY 2 cut(s) 682, 953
BstYI RGATCY 2 cut(s) 682, 953
BstZ17I GTATAC 1 cut(s) 805
BstZI CGGCCG 4 cut(s) 184, 211, 226, 714
Bsu15I ATCGAT 1 cut(s) 474
BsuI GTATCC 1 cut(s) 609
BsuRI GGCC 8 cut(s) 84, 100, 186, 213, 228, 631, 716, 888
BsuTUI ATCGAT 1 cut(s) 474
BtgZI GCGATG 1 cut(s) 960
BtrI CACGTC 1 cut(s) 918
BtsCI GGATG 6 cut(s) 399, 644, 698, 765, 784, 979
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 3 cut(s) 86, 860, 871
CaiI CAGNNNCTG 1 cut(s) 19
CciNI GCGGCCGC 1 cut(s) 714
CfoI GCGC 2 cut(s) 90, 744
Cfr10I RCCGGY 2 cut(s) 137, 209
Cfr13I GGNCC 4 cut(s) 99, 342, 629, 887
ClaI ATCGAT 1 cut(s) 474
Csp6I GTAC 4 cut(s) 189, 523, 817, 895
CspAI ACCGGT 1 cut(s) 137
CviAII CATG 5 cut(s) 391, 469, 689, 838, 870
CviQI GTAC 4 cut(s) 189, 523, 817, 895
DdeI CTNAG 2 cut(s) 12, 67
DpnI GATC 7 cut(s) 205, 473, 492, 594, 684, 822, 955
DpnII GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
DriI GACNNNNNGTC 1 cut(s) 921
EaeI YGGCCR 5 cut(s) 82, 184, 211, 226, 714
EagI CGGCCG 4 cut(s) 184, 211, 226, 714
Eam1104I CTCTTC 1 cut(s) 533
Eam1105I GACNNNNNGTC 1 cut(s) 921
EarI CTCTTC 1 cut(s) 533
EclXI CGGCCG 4 cut(s) 184, 211, 226, 714
Eco31I GGTCTC 2 cut(s) 29, 757
Eco47I GGWCC 1 cut(s) 342
Eco52I CGGCCG 4 cut(s) 184, 211, 226, 714
EcoO109I RGGNCCY 2 cut(s) 342, 887
EcoRI GAATTC 1 cut(s) 978
EcoRII CCWGG 1 cut(s) 18
FaeI CATG 5 cut(s) 394, 472, 692, 841, 873
FaqI GGGAC 2 cut(s) 134, 350
FatI CATG 5 cut(s) 390, 468, 688, 837, 869
FauI CCCGC 1 cut(s) 867
FbaI TGATCA 1 cut(s) 592
FblI GTMKAC 1 cut(s) 804
Fnu4HI GCNGC 8 cut(s) 214, 229, 561, 714, 717, 874, 877, 996
FokI GGATG 6 cut(s) 386, 651, 705, 772, 791, 986
Fsp4HI GCNGC 8 cut(s) 214, 229, 561, 714, 717, 874, 877, 996
FspBI CTAG 2 cut(s) 287, 855
GlaI GCGC 2 cut(s) 89, 743
GluI GCNGC 8 cut(s) 214, 229, 561, 714, 717, 874, 877, 996
GsaI CCCAGC 1 cut(s) 973
HaeIII GGCC 8 cut(s) 84, 100, 186, 213, 228, 631, 716, 888
HapII CCGG 4 cut(s) 138, 210, 454, 627
HhaI GCGC 2 cut(s) 90, 744
Hin1II CATG 5 cut(s) 394, 472, 692, 841, 873
Hin6I GCGC 2 cut(s) 88, 742
HinP1I GCGC 2 cut(s) 88, 742
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 5 cut(s) 122, 277, 290, 500, 543
HpaII CCGG 4 cut(s) 138, 210, 454, 627
HphI GGTGA 1 cut(s) 979
Hpy166II GTNNAC 4 cut(s) 143, 172, 312, 805
Hpy188I TCNGA 5 cut(s) 57, 96, 133, 223, 357
Hpy188III TCNNGA 4 cut(s) 454, 568, 667, 957
Hpy8I GTNNAC 4 cut(s) 143, 172, 312, 805
Hpy99I CGWCG 2 cut(s) 80, 227
HpyAV CCTTC 7 cut(s) 62, 362, 440, 667, 728, 769, 944
HpyCH4III ACNGT 1 cut(s) 848
HpyCH4IV ACGT 4 cut(s) 35, 361, 917, 963
HpyCH4V TGCA 7 cut(s) 9, 332, 394, 419, 560, 753, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 329, 581, 750, 885
HpyF3I CTNAG 2 cut(s) 12, 67
HpySE526I ACGT 4 cut(s) 35, 361, 917, 963
Hsp92II CATG 5 cut(s) 394, 472, 692, 841, 873
HspAI GCGC 2 cut(s) 88, 742
Kpn2I TCCGGA 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 592
Kzo9I GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
LmnI GCTCC 1 cut(s) 320
Lsp1109I GCAGC 3 cut(s) 572, 860, 982
LweI GCATC 1 cut(s) 769
MaeI CTAG 2 cut(s) 287, 855
MaeII ACGT 4 cut(s) 35, 361, 917, 963
MaeIII GTNAC 2 cut(s) 532, 704
MalI GATC 7 cut(s) 205, 473, 492, 594, 684, 822, 955
MboI GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
MboII GAAGA 5 cut(s) 31, 100, 187, 550, 682
MfeI CAATTG 1 cut(s) 770
MflI RGATCY 2 cut(s) 682, 953
MlsI TGGCCA 1 cut(s) 84
MluCI AATT 2 cut(s) 770, 978
MluNI TGGCCA 1 cut(s) 84
MlyI GAGTC 3 cut(s) 116, 284, 286
MmeI TCCRAC 2 cut(s) 150, 246
Mox20I TGGCCA 1 cut(s) 84
MroI TCCGGA 1 cut(s) 453
MscI TGGCCA 1 cut(s) 84
MslI CAYNNNNRTG 2 cut(s) 637, 691
Msp20I TGGCCA 1 cut(s) 84
MspI CCGG 4 cut(s) 138, 210, 454, 627
MspR9I CCNGG 2 cut(s) 20, 628
MunI CAATTG 1 cut(s) 770
Mva1269I GAATGC 1 cut(s) 1050
MvaI CCWGG 1 cut(s) 20
MvnI CGCG 3 cut(s) 713, 719, 1074
MwoI GCNNNNNNNGC 4 cut(s) 329, 581, 750, 885
NciI CCSGG 1 cut(s) 628
NdeII GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
NlaIII CATG 5 cut(s) 394, 472, 692, 841, 873
NlaIV GGNNCC 5 cut(s) 46, 322, 343, 684, 955
NmuCI GTSAC 1 cut(s) 532
NotI GCGGCCGC 1 cut(s) 714
NspI RCATGY 1 cut(s) 873
PaeI GCATGC 1 cut(s) 873
PasI CCCWGGG 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 1050
PfeI GAWTC 2 cut(s) 500, 543
Pfl23II CGTACG 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 137
PkrI GCNGC 8 cut(s) 215, 230, 562, 715, 718, 875, 878, 997
PleI GAGTC 3 cut(s) 116, 284, 285
PpsI GAGTC 3 cut(s) 116, 284, 285
Ppu21I YACGTR 1 cut(s) 964
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 18
PspFI CCCAGC 1 cut(s) 969
PspGI CCWGG 1 cut(s) 18
PspLI CGTACG 1 cut(s) 522
PspN4I GGNNCC 5 cut(s) 46, 322, 343, 684, 955
PspPI GGNCC 4 cut(s) 99, 342, 629, 887
PspPPI RGGWCCY 1 cut(s) 342
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 19
PsuI RGATCY 2 cut(s) 682, 953
RsaI GTAC 4 cut(s) 190, 524, 818, 896
RsaNI GTAC 4 cut(s) 189, 523, 817, 895
RseI CAYNNNNRTG 2 cut(s) 637, 691
SatI GCNGC 8 cut(s) 214, 229, 561, 714, 717, 874, 877, 996
Sau3AI GATC 7 cut(s) 203, 471, 490, 592, 682, 820, 953
Sau96I GGNCC 4 cut(s) 99, 342, 629, 887
SchI GAGTC 3 cut(s) 116, 284, 286
ScrFI CCNGG 2 cut(s) 20, 628
SfaNI GCATC 1 cut(s) 769
SfcI CTRYAG 2 cut(s) 417, 786
SgrAI CRCCGGYG 1 cut(s) 137
SinI GGWCC 1 cut(s) 342
SmiMI CAYNNNNRTG 2 cut(s) 637, 691
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
SphI GCATGC 1 cut(s) 873
Sse9I AATT 2 cut(s) 770, 978
SsiI CCGC 8 cut(s) 199, 214, 229, 713, 717, 860, 876, 1074
SspI AATATT 2 cut(s) 382, 1037
SspMI CTAG 2 cut(s) 287, 855
StyD4I CCNGG 2 cut(s) 18, 626
TaaI ACNGT 1 cut(s) 848
TaiI ACGT 4 cut(s) 38, 364, 920, 966
TaqI TCGA 4 cut(s) 78, 240, 474, 1016
TasI AATT 2 cut(s) 770, 978
TauI GCSGC 5 cut(s) 216, 231, 716, 719, 879
TfiI GAWTC 2 cut(s) 500, 543
TseFI GTSAC 1 cut(s) 532
TseI GCWGC 3 cut(s) 560, 873, 995
Tsp45I GTSAC 1 cut(s) 532
TspDTI ATGAA 5 cut(s) 388, 459, 854, 963, 971
TspGWI ACGGA 3 cut(s) 64, 157, 882
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 1 cut(s) 978
XceI RCATGY 1 cut(s) 873
XcmI CCANNNNNNNNNTGG 2 cut(s) 260, 752
XmiI GTMKAC 1 cut(s) 804
XspI CTAG 2 cut(s) 287, 855
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.