Rh3DG087200

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005861
Physical Location & Seq
Forward (+)
6767546 .. 6771527
3982 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG087200.1

Sequence Viewer

Length: 1308 bp
ATGAAAGCCACAGAGGAAGAAGAAGACTATGATCCTTTGTTGTTAGAGAATGGAAATCTTGAAAAGAAAGATAGGGAGGGTTTAGGCAATCCAACAGCGCCTTCTCTTAGTCATCAAGCATTGCATTTGCCAAAACATAATATAGCCTTTCATGAACAAATCCCAACTGAAGAAAATGAAAACTACAGTGTCACATCTCAACAGAAGAAGCAGCAGCAGCAGCATTGCTTCGCCGTCTCCATGGCTTTCTCTTTCTCCATCTCGGCCATATTGCTTCTCGTTCTCCTTTCATTCTCCGTCACCGGCCAGCAACCCAGCTATAAAGGCCCCATCATCTTCAACTTCGGAGACTCCAACTCCGACACGGGCGGACTTGTCGCTGGACTCGGCTACACCATCAACCCCCCTTACGGCCGCACCTACTTCCGGCGATCCACCGGCCGGATGTCCGACGGACGCCTCATCATCGACTTCCTCTGCCAAAGTTTGAATACAAGCCTATTGGCTCCATATCTAGACTCTCTGTCAGGGTCTAAGTTCACAAATGGGGCGAATTTTGCAATAGTCGGGTCGTCTATCTTCCCTAAACGCATCCCTTTCTCTTTGAGCATCCAAGTCATGCAGTTCCTTCATTTCAAAGCTACTGCAGGCTCAAAAGACTTGATAGATGATGAAGGTTTTCGGAAAGCACTTTACATGATCGATATTGGACAAAATGACCTTGCTCAGTTGTTTTCCAAAAATCTCTCGTTCACGCAAATAACAGAGAAAATCCCACCAATTACTACAGAAATCAAGAAAGCCGTTAAGACTATATATGACCAAGGCGGGCGGAATTTTTGGATACACAACACTGGGCCATTGGGTTGTCTCCCTCAAAAACTTTCATCAGTCCAGCAGAGGGACATGGATACATATGGATGCCTCTCGACCTACAATGCGGCTGCAAAATTGTTCAATGTAGGATTGCGTCATACATGCGATGAGATGAGATTTGAATTGAAGGATGTTAAAGTAGTATATGTAGATGTATATGCCATCAAATATGACCTCATTGCTAACTACACCAAATACGGTTTCACTAGTCCTCTAATGGCATGCTGTGGAAACGGAGGTCCTCCATACAACTACAACATGAGGCTGACATGCGGCTATCCCGGTTCTCAGGTTTGCGAGGATGGATCCCAACTTATGAGTTGGGATGGGATTCACTACACTGAAGCAGCAAATACCATTGTAGCTTCAAAAATACTTTCCACGGATTATTCTATACCGCCAATTCCATTTGATTTCTTTGTTCGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

435

Amino Acids

48.4

Weight (kDa)

6.15

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 112 - 417 5.4e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 7 cut(s) 369, 415, 828, 832, 941, 1149, 1274
AclWI GGATC 4 cut(s) 26, 426, 1176, 1189
AcoI YGGCCR 4 cut(s) 264, 304, 412, 439
AcsI RAATTY 2 cut(s) 553, 835
AcuI CTGAAG 2 cut(s) 189, 1239
AcyI GRCGYC 1 cut(s) 457
AfiI CCNNNNNNNGG 4 cut(s) 410, 426, 441, 901
AgsI TTSAA 8 cut(s) 62, 340, 490, 637, 958, 998, 1003, 1245
AhdI GACNNNNNGTC 1 cut(s) 523
AhlI ACTAGT 1 cut(s) 1082
AjuI GAANNNNNNNTTGG 4 cut(s) 85, 117, 1061, 1093
AluBI AGCT 3 cut(s) 318, 641, 1241
AluI AGCT 3 cut(s) 318, 641, 1241
Alw26I GTCTC 3 cut(s) 241, 342, 875
AlwI GGATC 4 cut(s) 26, 426, 1176, 1189
AoxI GGCC 6 cut(s) 264, 304, 325, 412, 439, 857
ApeKI GCWGC 6 cut(s) 211, 214, 217, 220, 944, 1223
ApoI RAATTY 2 cut(s) 553, 835
Asp700I GAANNNNTTC 1 cut(s) 678
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 3 cut(s) 326, 857, 1115
AsuC2I CCSGG 1 cut(s) 1158
AsuHPI GGTGA 1 cut(s) 292
AvaII GGWCC 1 cut(s) 1115
BamHI GGATCC 1 cut(s) 1181
BbsI GAAGAC 1 cut(s) 30
BbvI GCAGC 6 cut(s) 223, 226, 229, 232, 931, 1235
BccI CCATC 6 cut(s) 266, 338, 404, 1046, 1172, 1196
BceAI ACGGC 3 cut(s) 218, 427, 788
BciVI GTATCC 2 cut(s) 837, 904
BcnI CCSGG 1 cut(s) 1158
BcoDI GTCTC 3 cut(s) 241, 342, 875
BcuI ACTAGT 1 cut(s) 1082
BfaI CTAG 2 cut(s) 515, 1083
BfmI CTRYAG 3 cut(s) 184, 645, 786
BfoI RGCGCY 1 cut(s) 101
BfuI GTATCC 2 cut(s) 837, 904
BisI GCNGC 9 cut(s) 212, 215, 218, 221, 415, 942, 945, 1150, 1224
BlsI GCNGC 9 cut(s) 213, 216, 219, 222, 416, 943, 946, 1151, 1225
Bme1390I CCNGG 1 cut(s) 1158
Bme18I GGWCC 1 cut(s) 1115
BmeRI GACNNNNNGTC 1 cut(s) 523
BmgT120I GGNCC 3 cut(s) 326, 857, 1115
BmiI GGNNCC 3 cut(s) 328, 507, 1183
BmrFI CCNGG 1 cut(s) 1158
BmrI ACTGGG 1 cut(s) 864
BmsI GCATC 3 cut(s) 600, 618, 911
BmuI ACTGGG 1 cut(s) 864
BpiI GAAGAC 1 cut(s) 30
BpuMI CCSGG 1 cut(s) 1158
Bsa29I ATCGAT 1 cut(s) 702
BsaHI GRCGYC 1 cut(s) 457
BsaJI CCNNGG 3 cut(s) 240, 823, 1257
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 4 cut(s) 410, 426, 441, 901
Bse118I RCCGGY 2 cut(s) 302, 437
Bse1I ACTGG 1 cut(s) 859
Bse3DI GCAATG 3 cut(s) 119, 223, 1053
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 3 cut(s) 240, 823, 1257
BseGI GGATG 7 cut(s) 450, 591, 609, 926, 1012, 1183, 1207
BseLI CCNNNNNNNGG 4 cut(s) 410, 426, 441, 901
BseMI GCAATG 3 cut(s) 119, 223, 1053
BseMII CTCAG 2 cut(s) 740, 1178
BseNI ACTGG 1 cut(s) 859
BseX3I CGGCCG 2 cut(s) 412, 439
BseXI GCAGC 6 cut(s) 223, 226, 229, 232, 931, 1235
BseYI CCCAGC 1 cut(s) 314
Bsh1285I CGRYCG 2 cut(s) 415, 442
BshFI GGCC 6 cut(s) 266, 306, 327, 414, 441, 859
BshVI ATCGAT 1 cut(s) 702
BsiEI CGRYCG 2 cut(s) 415, 442
BsiSI CCGG 5 cut(s) 303, 427, 438, 442, 1158
BslFI GGGAC 1 cut(s) 917
BslI CCNNNNNNNGG 4 cut(s) 410, 426, 441, 901
BsmAI GTCTC 3 cut(s) 241, 342, 875
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 917
BsnI GGCC 6 cut(s) 266, 306, 327, 414, 441, 859
Bsp143I GATC 4 cut(s) 31, 431, 699, 1181
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 7 cut(s) 369, 415, 828, 832, 941, 1149, 1274
BspANI GGCC 6 cut(s) 266, 306, 327, 414, 441, 859
BspCNI CTCAG 2 cut(s) 739, 1177
BspDI ATCGAT 1 cut(s) 702
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 3 cut(s) 328, 507, 1183
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 4 cut(s) 26, 426, 1176, 1189
BsrDI GCAATG 3 cut(s) 119, 223, 1053
BsrFI RCCGGY 2 cut(s) 302, 437
BsrI ACTGG 1 cut(s) 859
BssAI RCCGGY 2 cut(s) 302, 437
BssECI CCNNGG 3 cut(s) 240, 823, 1257
BssMI GATC 4 cut(s) 31, 431, 699, 1181
BssNI GRCGYC 1 cut(s) 457
BssT1I CCWWGG 2 cut(s) 240, 823
Bst4CI ACNGT 2 cut(s) 188, 1076
BstACI GRCGYC 1 cut(s) 457
BstC8I GCNNGC 4 cut(s) 308, 649, 830, 1099
BstDEI CTNAG 4 cut(s) 107, 534, 726, 1164
BstDSI CCRYGG 2 cut(s) 240, 1257
BstF5I GGATG 7 cut(s) 450, 591, 609, 926, 1012, 1183, 1207
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 4 cut(s) 34, 434, 702, 1184
BstMAI GTCTC 3 cut(s) 241, 342, 875
BstMBI GATC 4 cut(s) 31, 431, 699, 1181
BstMCI CGRYCG 2 cut(s) 415, 442
BstMWI GCNNNNNNNGC 4 cut(s) 217, 220, 324, 557
BstNSI RCATGY 3 cut(s) 981, 1101, 1149
BstSCI CCNGG 1 cut(s) 1156
BstSFI CTRYAG 3 cut(s) 184, 645, 786
BstV1I GCAGC 6 cut(s) 223, 226, 229, 232, 931, 1235
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 1181
BstYI RGATCY 1 cut(s) 1181
BstZI CGGCCG 2 cut(s) 412, 439
Bsu15I ATCGAT 1 cut(s) 702
BsuI GTATCC 2 cut(s) 837, 904
BsuRI GGCC 6 cut(s) 266, 306, 327, 414, 441, 859
BsuTUI ATCGAT 1 cut(s) 702
BtgI CCRYGG 2 cut(s) 240, 1257
BtgZI GCGATG 1 cut(s) 996
BtsCI GGATG 7 cut(s) 450, 591, 609, 926, 1012, 1183, 1207
BtsIMutI CAGTG 3 cut(s) 193, 852, 1215
Cac8I GCNNGC 4 cut(s) 308, 649, 830, 1099
CciI TCATGA 1 cut(s) 151
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 2 cut(s) 302, 437
Cfr13I GGNCC 3 cut(s) 326, 857, 1115
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 2 cut(s) 465, 959
CviAII CATG 9 cut(s) 152, 241, 619, 697, 907, 978, 1098, 1135, 1146
DdeI CTNAG 4 cut(s) 107, 534, 726, 1164
DpnI GATC 4 cut(s) 33, 433, 701, 1183
DpnII GATC 4 cut(s) 31, 431, 699, 1181
DriI GACNNNNNGTC 1 cut(s) 523
EaeI YGGCCR 4 cut(s) 264, 304, 412, 439
EagI CGGCCG 2 cut(s) 412, 439
Eam1105I GACNNNNNGTC 1 cut(s) 523
EciI GGCGGA 2 cut(s) 384, 847
EclXI CGGCCG 2 cut(s) 412, 439
Eco130I CCWWGG 2 cut(s) 240, 823
Eco47I GGWCC 1 cut(s) 1115
Eco52I CGGCCG 2 cut(s) 412, 439
Eco57I CTGAAG 2 cut(s) 189, 1239
EcoO109I RGGNCCY 2 cut(s) 326, 1115
EcoT14I CCWWGG 2 cut(s) 240, 823
ErhI CCWWGG 2 cut(s) 240, 823
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 9 cut(s) 155, 244, 622, 700, 910, 981, 1101, 1138, 1149
FaqI GGGAC 1 cut(s) 917
FatI CATG 9 cut(s) 151, 240, 618, 696, 906, 977, 1097, 1134, 1145
FauI CCCGC 1 cut(s) 821
FauNDI CATATG 1 cut(s) 916
Fnu4HI GCNGC 9 cut(s) 212, 215, 218, 221, 415, 942, 945, 1150, 1224
FokI GGATG 7 cut(s) 457, 578, 596, 933, 1019, 1190, 1214
Fsp4HI GCNGC 9 cut(s) 212, 215, 218, 221, 415, 942, 945, 1150, 1224
FspBI CTAG 2 cut(s) 515, 1083
GlaI GCGC 1 cut(s) 99
GluI GCNGC 9 cut(s) 212, 215, 218, 221, 415, 942, 945, 1150, 1224
GsaI CCCAGC 1 cut(s) 318
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 6 cut(s) 266, 306, 327, 414, 441, 859
HapII CCGG 5 cut(s) 303, 427, 438, 442, 1158
HgaI GACGC 2 cut(s) 465, 959
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 457
Hin1II CATG 9 cut(s) 155, 244, 622, 700, 910, 981, 1101, 1138, 1149
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HinfI GANTC 4 cut(s) 350, 384, 518, 1207
HpaII CCGG 5 cut(s) 303, 427, 438, 442, 1158
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 540, 753
Hpy188I TCNGA 4 cut(s) 347, 361, 451, 684
Hpy188III TCNNGA 5 cut(s) 59, 152, 515, 796, 928
Hpy8I GTNNAC 2 cut(s) 540, 753
Hpy99I CGWCG 1 cut(s) 455
HpyAV CCTTC 4 cut(s) 111, 638, 668, 997
HpyCH4III ACNGT 2 cut(s) 188, 1076
HpyCH4V TGCA 5 cut(s) 124, 560, 622, 647, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 217, 220, 324, 557
HpyF3I CTNAG 4 cut(s) 107, 534, 726, 1164
Hsp92I GRCGYC 1 cut(s) 457
Hsp92II CATG 9 cut(s) 155, 244, 622, 700, 910, 981, 1101, 1138, 1149
HspAI GCGC 1 cut(s) 98
Kzo9I GATC 4 cut(s) 31, 431, 699, 1181
LmnI GCTCC 1 cut(s) 511
Lsp1109I GCAGC 6 cut(s) 223, 226, 229, 232, 931, 1235
LweI GCATC 3 cut(s) 600, 618, 911
MaeI CTAG 2 cut(s) 515, 1083
MaeIII GTNAC 2 cut(s) 190, 298
MalI GATC 4 cut(s) 33, 433, 701, 1183
MboI GATC 4 cut(s) 31, 431, 699, 1181
MboII GAAGA 7 cut(s) 29, 32, 35, 182, 217, 328, 571
MflI RGATCY 1 cut(s) 1181
MluCI AATT 6 cut(s) 553, 780, 835, 950, 998, 1278
MlyI GAGTC 3 cut(s) 344, 378, 512
MmeI TCCRAC 4 cut(s) 116, 378, 384, 474
MroXI GAANNNNTTC 1 cut(s) 678
MseI TTAA 2 cut(s) 807, 1011
MslI CAYNNNNRTG 1 cut(s) 919
MspI CCGG 5 cut(s) 303, 427, 438, 442, 1158
MspR9I CCNGG 1 cut(s) 1158
MwoI GCNNNNNNNGC 4 cut(s) 217, 220, 324, 557
NciI CCSGG 1 cut(s) 1158
NcoI CCATGG 1 cut(s) 240
NdeI CATATG 1 cut(s) 916
NdeII GATC 4 cut(s) 31, 431, 699, 1181
NlaIII CATG 9 cut(s) 155, 244, 622, 700, 910, 981, 1101, 1138, 1149
NlaIV GGNNCC 3 cut(s) 328, 507, 1183
NmeAIII GCCGAG 2 cut(s) 242, 366
NmuCI GTSAC 2 cut(s) 190, 298
NspI RCATGY 3 cut(s) 981, 1101, 1149
PaeI GCATGC 1 cut(s) 1101
PagI TCATGA 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 678
PfeI GAWTC 1 cut(s) 1207
PkrI GCNGC 9 cut(s) 213, 216, 219, 222, 416, 943, 946, 1151, 1225
PleI GAGTC 3 cut(s) 344, 378, 512
PpsI GAGTC 3 cut(s) 344, 378, 512
PpuMI RGGWCCY 1 cut(s) 1115
Psp5II RGGWCCY 1 cut(s) 1115
PspFI CCCAGC 1 cut(s) 314
PspN4I GGNNCC 3 cut(s) 328, 507, 1183
PspPI GGNCC 3 cut(s) 326, 857, 1115
PspPPI RGGWCCY 1 cut(s) 1115
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 1 cut(s) 1181
RseI CAYNNNNRTG 1 cut(s) 919
SaqAI TTAA 2 cut(s) 807, 1011
SatI GCNGC 9 cut(s) 212, 215, 218, 221, 415, 942, 945, 1150, 1224
Sau3AI GATC 4 cut(s) 31, 431, 699, 1181
Sau96I GGNCC 3 cut(s) 326, 857, 1115
SchI GAGTC 3 cut(s) 344, 378, 512
ScrFI CCNGG 1 cut(s) 1158
SfaNI GCATC 3 cut(s) 600, 618, 911
SfcI CTRYAG 3 cut(s) 184, 645, 786
SinI GGWCC 1 cut(s) 1115
SmiMI CAYNNNNRTG 1 cut(s) 919
SpeI ACTAGT 1 cut(s) 1082
SphI GCATGC 1 cut(s) 1101
Sse9I AATT 6 cut(s) 553, 780, 835, 950, 998, 1278
SsiI CCGC 7 cut(s) 369, 415, 828, 832, 941, 1149, 1274
SspMI CTAG 2 cut(s) 515, 1083
StyD4I CCNGG 1 cut(s) 1156
StyI CCWWGG 2 cut(s) 240, 823
TaaI ACNGT 2 cut(s) 188, 1076
TaqI TCGA 3 cut(s) 468, 702, 929
TasI AATT 6 cut(s) 553, 780, 835, 950, 998, 1278
TauI GCSGC 3 cut(s) 417, 944, 1152
TfiI GAWTC 1 cut(s) 1207
Tru1I TTAA 2 cut(s) 807, 1011
Tru9I TTAA 2 cut(s) 807, 1011
TscAI CASTG 3 cut(s) 193, 859, 1222
TseFI GTSAC 2 cut(s) 190, 298
TseI GCWGC 6 cut(s) 211, 214, 217, 220, 944, 1223
Tsp45I GTSAC 2 cut(s) 190, 298
TspDTI ATGAA 8 cut(s) 17, 140, 168, 192, 279, 620, 687, 876
TspGWI ACGGA 4 cut(s) 286, 468, 1125, 1274
TspRI CASTG 3 cut(s) 193, 859, 1222
VpaK11BI GGWCC 1 cut(s) 1115
XapI RAATTY 2 cut(s) 553, 835
XbaI TCTAGA 1 cut(s) 514
XceI RCATGY 3 cut(s) 981, 1101, 1149
XmnI GAANNNNTTC 1 cut(s) 678
XspI CTAG 2 cut(s) 515, 1083
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.