AT1G24267

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004665
Physical Location & Seq
Reverse (-)
8604022 .. 8607484
3463 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G24267.2

Sequence Viewer

Length: 1032 bp
ATGGCTATTCCTCTCGGGAAGCTCACCATCCTCATCGGTGCAGGTCTTGTTGGGTCAGTACTTGCTAAAGAAGGGAGTTTACCAGATGTCTCGAGTTTTGTCTCCGGTGCTCTTAAGATGGTATTTAGGCAGCTGAAGCAAGAAGAACCTGCCAAATCAGCCAGCAAGCCCCGCAATGATACACTAATGGCCCAGGTTAACAGTCTTAGGCATGAATTGAGCTTGCTTTCTTCAAACAGACCTATCACTATTGTTACCACAGCAGGATCAGGTGGTAAAAAGTATGGTTACATCATTATAATTGGGGTGATAGGCTACGGATATGTATGGTGGAAGGGCTGGAAACTTCCGGATCTTATGTTTGCAACAAGGCGCAGCTTATCAGATGCATGTAATAGTGTTGGCAGCCAGATCGATGGTTTTTACACATCACTCTCGGGTACCAAAAAAGAGTTAAGTTCAAAAATTGACGGGATGGGTCGTAGTTTGGATGCTAATACAGAAATTATTCAAGATACAGGACGAGAGGTGATGGAACTACAAAGAGGTACGGAAAATATTAAGGATGACGTTAAGTTTGTTTTCGATGCTGTTGAAAATCTGGCAAGCAAAGTCTATCGTATTGAGGGAAATCAGGATATTACACTTAAAGGAGTCGGGGCGCTGCATGCTCAGGTTCGGGAGAACAAAAGAATTCAAGAGTCCAATAAGGCTTTACCATCAACTTCAGCAGTGCCAGCCCTTGAGGCAGCTCCTATGACACCATCGTCAAGGACTTTGTCTTTGCCTCCTGCTTCTCCCCGTGAATCTCAGTCGCCTTCAACTTCTAATGGAGCTCAACAGTCACGTGGTCCACTTCAGCACACTCAATCTATGTCTGGTTTGAAGGAGATAAGCGAAAATGGAACACATTCTGGAGAAACTACGGGAAACACGAGTTCTGGCCTGTTTAGTATTTTCTCAATACCAAGAATAGGGAGGACTCGAAGTGTGGTCAACACAGTGCCTACTAACTCCATTGGACCCCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

343

Amino Acids

36.68

Weight (kDa)

9.49

Isoelectric Point (pI)

44.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 91 - 213 5.8e-43 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 299
AasI GACNNNNNNGTC 1 cut(s) 766
Acc36I ACCTGC 2 cut(s) 32, 157
Acc65I GGTACC 1 cut(s) 440
AccB1I GGYRCC 1 cut(s) 440
AccIII TCCGGA 1 cut(s) 349
AciI CCGC 1 cut(s) 172
AclWI GGATC 2 cut(s) 274, 360
AcsI RAATTY 1 cut(s) 693
AcuI CTGAAG 3 cut(s) 155, 711, 842
AcvI CACGTG 1 cut(s) 848
AfaI GTAC 3 cut(s) 60, 442, 550
AfiI CCNNNNNNNGG 1 cut(s) 974
AflII CTTAAG 1 cut(s) 113
AgsI TTSAA 7 cut(s) 234, 462, 512, 596, 698, 822, 886
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 6 cut(s) 22, 133, 222, 378, 752, 836
AluI AGCT 6 cut(s) 22, 133, 222, 378, 752, 836
Alw21I GWGCWC 2 cut(s) 112, 838
Alw26I GTCTC 2 cut(s) 94, 106
AlwI GGATC 2 cut(s) 274, 360
Ama87I CYCGRG 3 cut(s) 14, 91, 436
Aor13HI TCCGGA 1 cut(s) 349
AoxI GGCC 2 cut(s) 189, 943
ApeKI GCWGC 5 cut(s) 130, 375, 405, 664, 749
ApoI RAATTY 1 cut(s) 693
ArsI GACNNNNNNTTYG 4 cut(s) 560, 592, 766, 798
Asp700I GAANNNNTTC 2 cut(s) 507, 910
Asp718I GGTACC 1 cut(s) 440
AspLEI GCGC 2 cut(s) 375, 664
AspS9I GGNCC 3 cut(s) 190, 851, 1022
AsuHPI GGTGA 3 cut(s) 16, 319, 541
AvaI CYCGRG 3 cut(s) 14, 91, 436
AvaII GGWCC 2 cut(s) 851, 1022
BanI GGYRCC 1 cut(s) 440
BanII GRGCYC 1 cut(s) 838
BarI GAAGNNNNNNTAC 2 cut(s) 63, 95
BauI CACGAG 1 cut(s) 934
BbrPI CACGTG 1 cut(s) 848
Bbv12I GWGCWC 2 cut(s) 112, 838
BbvI GCAGC 5 cut(s) 142, 387, 417, 651, 761
BccI CCATC 7 cut(s) 35, 112, 410, 469, 526, 727, 772
BcgI CGANNNNNNTGC 2 cut(s) 394, 428
BciT130I CCWGG 1 cut(s) 194
BcoDI GTCTC 2 cut(s) 94, 106
BfoI RGCGCY 1 cut(s) 665
BfrI CTTAAG 1 cut(s) 113
BfuAI ACCTGC 2 cut(s) 32, 157
BglI GCCNNNNNGGC 1 cut(s) 746
BisI GCNGC 5 cut(s) 131, 376, 406, 665, 750
BlsI GCNGC 5 cut(s) 132, 377, 407, 666, 751
BmcAI AGTACT 1 cut(s) 60
Bme1390I CCNGG 1 cut(s) 194
Bme18I GGWCC 2 cut(s) 851, 1022
BmeT110I CYCGRG 3 cut(s) 14, 91, 436
BmgT120I GGNCC 3 cut(s) 190, 851, 1022
BmiI GGNNCC 2 cut(s) 442, 1024
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 3 cut(s) 376, 481, 577
BpmI CTGGAG 1 cut(s) 936
Bpu10I CCTNAGC 1 cut(s) 672
BpuEI CTTGAG 1 cut(s) 764
Bsa29I ATCGAT 1 cut(s) 414
BsaAI YACGTR 1 cut(s) 848
BsaJI CCNNGG 1 cut(s) 192
BsaWI WCCGGW 2 cut(s) 104, 349
Bsc4I CCNNNNNNNGG 1 cut(s) 974
Bse3DI GCAATG 1 cut(s) 181
BseAI TCCGGA 1 cut(s) 349
BseBI CCWGG 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 414
BseDI CCNNGG 1 cut(s) 192
BseGI GGATG 4 cut(s) 27, 480, 496, 571
BseLI CCNNNNNNNGG 1 cut(s) 974
BseMI GCAATG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 686, 824
BseXI GCAGC 5 cut(s) 142, 387, 417, 651, 761
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 2 cut(s) 191, 945
BshNI GGYRCC 1 cut(s) 440
BshVI ATCGAT 1 cut(s) 414
BsiHKAI GWGCWC 2 cut(s) 112, 838
BsiHKCI CYCGRG 3 cut(s) 14, 91, 436
BsiSI CCGG 2 cut(s) 105, 350
BslI CCNNNNNNNGG 1 cut(s) 974
BsmAI GTCTC 2 cut(s) 94, 106
BsnI GGCC 2 cut(s) 191, 945
BsoBI CYCGRG 3 cut(s) 14, 91, 436
Bsp1286I GDGCHC 2 cut(s) 112, 838
Bsp13I TCCGGA 1 cut(s) 349
Bsp143I GATC 3 cut(s) 266, 352, 411
BspACI CCGC 1 cut(s) 172
BspANI GGCC 2 cut(s) 191, 945
BspCNI CTCAG 2 cut(s) 685, 823
BspDI ATCGAT 1 cut(s) 414
BspEI TCCGGA 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 442, 1024
BspMI ACCTGC 2 cut(s) 32, 157
BspPI GGATC 2 cut(s) 274, 360
BspT107I GGYRCC 1 cut(s) 440
BspTI CTTAAG 1 cut(s) 113
BsrDI GCAATG 1 cut(s) 181
BssECI CCNNGG 1 cut(s) 192
BssMI GATC 3 cut(s) 266, 352, 411
BssSI CACGAG 1 cut(s) 934
Bst2BI CACGAG 1 cut(s) 934
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 3 cut(s) 203, 843, 1003
BstAFI CTTAAG 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 848
BstC8I GCNNGC 6 cut(s) 163, 167, 224, 607, 669, 738
BstDEI CTNAG 3 cut(s) 206, 672, 810
BstF5I GGATG 4 cut(s) 27, 480, 496, 571
BstH2I RGCGCY 1 cut(s) 665
BstHHI GCGC 2 cut(s) 375, 664
BstKTI GATC 3 cut(s) 269, 355, 414
BstMAI GTCTC 2 cut(s) 94, 106
BstMBI GATC 3 cut(s) 266, 352, 411
BstMWI GCNNNNNNNGC 6 cut(s) 136, 158, 171, 668, 737, 746
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 2 cut(s) 393, 671
BstSCI CCNGG 1 cut(s) 192
BstV1I GCAGC 5 cut(s) 142, 387, 417, 651, 761
BstX2I RGATCY 1 cut(s) 352
BstXI CCANNNNNNTGG 1 cut(s) 416
BstYI RGATCY 1 cut(s) 352
Bsu15I ATCGAT 1 cut(s) 414
BsuRI GGCC 2 cut(s) 191, 945
BsuTUI ATCGAT 1 cut(s) 414
BtsCI GGATG 4 cut(s) 27, 480, 496, 571
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 2 cut(s) 738, 1008
BveI ACCTGC 2 cut(s) 32, 157
Cac8I GCNNGC 6 cut(s) 163, 167, 224, 607, 669, 738
CfoI GCGC 2 cut(s) 375, 664
Cfr13I GGNCC 3 cut(s) 190, 851, 1022
ClaI ATCGAT 1 cut(s) 414
Csp6I GTAC 3 cut(s) 59, 441, 549
CviAII CATG 3 cut(s) 212, 390, 668
CviQI GTAC 3 cut(s) 59, 441, 549
DdeI CTNAG 3 cut(s) 206, 672, 810
DpnI GATC 3 cut(s) 268, 354, 413
DpnII GATC 3 cut(s) 266, 352, 411
DrdI GACNNNNNNGTC 1 cut(s) 766
DseDI GACNNNNNNGTC 1 cut(s) 766
Ecl136II GAGCTC 1 cut(s) 836
Eco24I GRGCYC 1 cut(s) 838
Eco47I GGWCC 2 cut(s) 851, 1022
Eco53kI GAGCTC 1 cut(s) 836
Eco57I CTGAAG 3 cut(s) 155, 711, 842
Eco72I CACGTG 1 cut(s) 848
Eco88I CYCGRG 3 cut(s) 14, 91, 436
EcoICRI GAGCTC 1 cut(s) 836
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 1 cut(s) 192
EcoT22I ATGCAT 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 838
FaeI CATG 3 cut(s) 215, 393, 671
FatI CATG 3 cut(s) 211, 389, 667
FauI CCCGC 1 cut(s) 179
Fnu4HI GCNGC 5 cut(s) 131, 376, 406, 665, 750
FokI GGATG 4 cut(s) 14, 487, 503, 578
FriOI GRGCYC 1 cut(s) 838
Fsp4HI GCNGC 5 cut(s) 131, 376, 406, 665, 750
GlaI GCGC 2 cut(s) 374, 663
GluI GCNGC 5 cut(s) 131, 376, 406, 665, 750
GsuI CTGGAG 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 665
HaeIII GGCC 2 cut(s) 191, 945
HapII CCGG 2 cut(s) 105, 350
HhaI GCGC 2 cut(s) 375, 664
Hin1II CATG 3 cut(s) 215, 393, 671
Hin6I GCGC 2 cut(s) 373, 662
HinP1I GCGC 2 cut(s) 373, 662
HincII GTYRAC 2 cut(s) 199, 997
HindII GTYRAC 2 cut(s) 199, 997
HinfI GANTC 4 cut(s) 654, 701, 806, 982
HpaI GTTAAC 1 cut(s) 199
HpaII CCGG 2 cut(s) 105, 350
HphI GGTGA 3 cut(s) 16, 319, 541
Hpy166II GTNNAC 4 cut(s) 80, 199, 854, 997
Hpy188I TCNGA 1 cut(s) 385
Hpy188III TCNNGA 8 cut(s) 16, 91, 350, 512, 635, 680, 698, 915
Hpy8I GTNNAC 4 cut(s) 80, 199, 854, 997
HpyAV CCTTC 4 cut(s) 65, 328, 828, 880
HpyCH4III ACNGT 3 cut(s) 203, 843, 1003
HpyCH4IV ACGT 2 cut(s) 570, 847
HpyCH4V TGCA 4 cut(s) 41, 365, 389, 667
HpyF10VI GCNNNNNNNGC 6 cut(s) 136, 158, 171, 668, 737, 746
HpyF3I CTNAG 3 cut(s) 206, 672, 810
HpySE526I ACGT 2 cut(s) 570, 847
Hsp92II CATG 3 cut(s) 215, 393, 671
HspAI GCGC 2 cut(s) 373, 662
Kpn2I TCCGGA 1 cut(s) 349
KpnI GGTACC 1 cut(s) 444
KspAI GTTAAC 1 cut(s) 199
Kzo9I GATC 3 cut(s) 266, 352, 411
LmnI GCTCC 2 cut(s) 757, 833
Lsp1109I GCAGC 5 cut(s) 142, 387, 417, 651, 761
LweI GCATC 3 cut(s) 376, 481, 577
MaeII ACGT 2 cut(s) 570, 847
MaeIII GTNAC 3 cut(s) 253, 287, 843
MalI GATC 3 cut(s) 268, 354, 413
MboI GATC 3 cut(s) 266, 352, 411
MboII GAAGA 2 cut(s) 155, 222
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 2 cut(s) 112, 838
MluCI AATT 5 cut(s) 215, 300, 465, 504, 693
MlyI GAGTC 3 cut(s) 663, 710, 976
MnlI CCTC 8 cut(s) 21, 41, 520, 539, 619, 739, 798, 972
Mph1103I ATGCAT 1 cut(s) 391
MroI TCCGGA 1 cut(s) 349
MroXI GAANNNNTTC 2 cut(s) 507, 910
MseI TTAA 6 cut(s) 114, 198, 455, 561, 573, 648
MspA1I CMGCKG 1 cut(s) 133
MspCI CTTAAG 1 cut(s) 113
MspI CCGG 2 cut(s) 105, 350
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 6 cut(s) 136, 158, 171, 668, 737, 746
NdeII GATC 3 cut(s) 266, 352, 411
NlaIII CATG 3 cut(s) 215, 393, 671
NlaIV GGNNCC 2 cut(s) 442, 1024
NmuCI GTSAC 1 cut(s) 843
NsiI ATGCAT 1 cut(s) 391
NspI RCATGY 2 cut(s) 393, 671
PaeI GCATGC 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 91
PcsI WCGNNNNNNNCGW 1 cut(s) 932
PdmI GAANNNNTTC 2 cut(s) 507, 910
PfeI GAWTC 1 cut(s) 806
PflFI GACNNNGTC 1 cut(s) 778
PkrI GCNGC 5 cut(s) 132, 377, 407, 666, 751
PleI GAGTC 3 cut(s) 662, 709, 976
PmaCI CACGTG 1 cut(s) 848
PmlI CACGTG 1 cut(s) 848
PpsI GAGTC 3 cut(s) 662, 709, 976
Ppu21I YACGTR 1 cut(s) 848
PsiI TTATAA 1 cut(s) 299
Psp124BI GAGCTC 1 cut(s) 838
Psp6I CCWGG 1 cut(s) 192
PspCI CACGTG 1 cut(s) 848
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 2 cut(s) 442, 1024
PspPI GGNCC 3 cut(s) 190, 851, 1022
PsuI RGATCY 1 cut(s) 352
PsyI GACNNNGTC 1 cut(s) 778
PvuII CAGCTG 1 cut(s) 133
RsaI GTAC 3 cut(s) 60, 442, 550
RsaNI GTAC 3 cut(s) 59, 441, 549
SacI GAGCTC 1 cut(s) 838
SaqAI TTAA 6 cut(s) 114, 198, 455, 561, 573, 648
SatI GCNGC 5 cut(s) 131, 376, 406, 665, 750
Sau3AI GATC 3 cut(s) 266, 352, 411
Sau96I GGNCC 3 cut(s) 190, 851, 1022
ScaI AGTACT 1 cut(s) 60
SchI GAGTC 3 cut(s) 663, 710, 976
ScrFI CCNGG 1 cut(s) 194
SduI GDGCHC 2 cut(s) 112, 838
SfaNI GCATC 3 cut(s) 376, 481, 577
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 2 cut(s) 851, 1022
SlaI CTCGAG 1 cut(s) 91
SmlI CTYRAG 3 cut(s) 91, 113, 743
SmoI CTYRAG 3 cut(s) 91, 113, 743
SphI GCATGC 1 cut(s) 671
Sse9I AATT 5 cut(s) 215, 300, 465, 504, 693
SsiI CCGC 1 cut(s) 172
SspI AATATT 1 cut(s) 559
SstI GAGCTC 1 cut(s) 838
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 3 cut(s) 203, 843, 1003
TaiI ACGT 2 cut(s) 573, 850
TaqI TCGA 4 cut(s) 92, 414, 585, 985
TasI AATT 5 cut(s) 215, 300, 465, 504, 693
TatI WGTACW 1 cut(s) 58
TfiI GAWTC 1 cut(s) 806
Tru1I TTAA 6 cut(s) 114, 198, 455, 561, 573, 648
Tru9I TTAA 6 cut(s) 114, 198, 455, 561, 573, 648
TscAI CASTG 2 cut(s) 738, 1008
TseFI GTSAC 1 cut(s) 843
TseI GCWGC 5 cut(s) 130, 375, 405, 664, 749
Tsp45I GTSAC 1 cut(s) 843
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 2 cut(s) 333, 566
TspRI CASTG 2 cut(s) 738, 1008
Tth111I GACNNNGTC 1 cut(s) 778
Vha464I CTTAAG 1 cut(s) 113
VpaK11BI GGWCC 2 cut(s) 851, 1022
XapI RAATTY 1 cut(s) 693
XceI RCATGY 2 cut(s) 393, 671
XhoI CTCGAG 1 cut(s) 91
XmnI GAANNNNTTC 2 cut(s) 507, 910
ZrmI AGTACT 1 cut(s) 60
Zsp2I ATGCAT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.