Rorug04G0238700

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004665
Physical Location & Seq
Forward (+)
40588855 .. 40590636
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0238700.1

Sequence Viewer

Length: 1782 bp
ATGATTGATGAAACAGACCAAGTGGTTCATTGCAGAGTTCTTATTCTGGACAGTAATGAGGATTTTCTTGTCTCCTTTATCTATGGAGCCAATGATGATAACGATCGCACTAACTTGTGGAATAATTTATCCTCTTTTGCTAATAGCCATGGGAATGTTCCTTGGCTTATTGTGGGAGACTTTAATGTTGTTAGATTCACTACTGAAATAAATGGTGGTAATGAGGCCCGGTCGAATGCTATGGATGAGTTTGCTTCTTGCATCCATGATACTGAACTTGAGGACTTAAATTATTCTGGTTGCAAGCTTACTTGGTGCAACAATCAATACGATGGTACTCTTATTCACAGGAAGCTGGATAGAGTTATGGGAAATAATGCTTGGCTTTCCAAATTCTCTAACTGCTTGGTAAAGTTTCTACCTCCTGGTATTTCTGATCATTGTCCTGCTATTGTTTCTCTTGGTACTCCAGTCCAGGGTAGGAAGTCCCCGTTCAAGTTTTTCAATTTTCTTGCTGAGAGGGAAGATTTCTTACCTATTGTTATGGAAGCTTGGAACACTGTTATTGTTGGGTCCTTCCAATATCAAGTTGCTAAGAAGCTCAAAATTATCAAAGCTGAGTTTAAAAAGCTTAATCGTATTAAAATTCAAGATGTTTCTGTTAGTGTAATTGCTGCTAAGGATGCTCTTGATGCCTGCCAGCAGAACTTGTATTCCAATCCCTTGGATCCCATTCTCAGATCACTAGAGCGTGACTTGCTACAGGCTTACTCTAATGCTCTTATTTGTGAGGAAAGTTTCTTCAGACAAAAGTCCAGGGTGCAGTGGCTTTCCTCTGGTGATAGTTGTACTTCTTTTTTCTTTAAAGTGGTAAACAAAAGACATTATAGGAACCGAATCAACTCCATTTGTCGTGGGGATGGGTCTGTTGCTATTACTGATGAGGATATTAAGGTAGAAGCTGTTGGTTTTTTCCAATCTTTATTTGGTGGGTCTAATTCCTCCCCTAGGCCAACCTTTGATGATCTTCAAAATATTATTCATAACCCTCTCTCTACTGAGCAATCCTCTGCTCTTGGTTGTGATTTTTCTGATGATGAGATTAAAGAGGTTTGCTTTGCTCTTCCTTCTAATAAAGCTCCTGGTCCTGATGGTTTTAATGCTTTCTTTTTTAAGAAGACTTGGCATATTATTGGCTGTGATATTATTAAAGCGGTCCAGGAGTTTTTGTCTTCTCACAAGCTTCTCCGGGAGTTCAATGCTACCATTATTACTTTGGTTCCTAAAGTCTGCAATCCTTCTTCCATGAGTGATTATAGGCCCATCTCTTGCTGCAATACCATTTATAAGATTATTTCTAAGTTGTTGGCTAATCGGGTGAAGTCTTGCTTACCTTCTATTATTAATCATTCTCAATCAGCTTTTATTGCTGGAAGGAGAATTGGAGATAATATTTTGTTGATGCAGGAGTTGCTTAAGAACTACCATAGGAACTCGGGTCCTCCCAGGTGTGCTATCAAAGTTGACATTTTGAAAGCTTATGACACAGTTGATTGGGATTTCATTCTTCATGCTCTTAGTGCTTTCAACTTCCCAGATTCTTTGGTTAGTTGGATAAGATGCTGCATCACGAGCCCAATGTTCTCTGTTTCCATTAATGGTGAGCTTGCTGGTTTCTTTCCTAGTAATCGTGGTTTGAGACAAGGAGACCCGCTTTCTCCTTACCTTTTTGTTATTGCTATATATGGAGGTTCTTTCTCGGCTCTTACATCACAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

593

Amino Acids

66.58

Weight (kDa)

6.47

Isoelectric Point (pI)

40.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 436 - 582 3.4e-26 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1347
AciI CCGC 2 cut(s) 1214, 1712
AclWI GGATC 2 cut(s) 722, 735
AcsI RAATTY 2 cut(s) 392, 645
AcuI CTGAAG 1 cut(s) 787
AfaI GTAC 3 cut(s) 337, 466, 850
AfiI CCNNNNNNNGG 2 cut(s) 476, 1008
AflII CTTAAG 1 cut(s) 1475
AgsI TTSAA 7 cut(s) 496, 505, 650, 1031, 1258, 1534, 1588
AjnI CCWGG 6 cut(s) 424, 474, 815, 1141, 1218, 1505
AjuI GAANNNNNNNTTGG 2 cut(s) 364, 396
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
Alw26I GTCTC 4 cut(s) 76, 171, 1693, 1701
AlwI GGATC 2 cut(s) 722, 735
Ama87I CYCGRG 1 cut(s) 1495
AoxI GGCC 3 cut(s) 225, 1010, 1319
ApeKI GCWGC 3 cut(s) 674, 1332, 1623
ApoI RAATTY 2 cut(s) 392, 645
AseI ATTAAT 2 cut(s) 1404, 1656
AspA2I CCTAGG 1 cut(s) 1007
AspS9I GGNCC 6 cut(s) 226, 573, 1145, 1216, 1320, 1499
AsuC2I CCSGG 2 cut(s) 229, 1250
AsuHPI GGTGA 3 cut(s) 851, 1390, 1673
AvaI CYCGRG 1 cut(s) 1495
AvaII GGWCC 4 cut(s) 573, 1145, 1216, 1499
AvrII CCTAGG 1 cut(s) 1007
BaeI ACNNNNGTAYC 2 cut(s) 456, 489
BamHI GGATCC 1 cut(s) 727
BanII GRGCYC 1 cut(s) 1637
BarI GAAGNNNNNNTAC 2 cut(s) 516, 548
BauI CACGAG 1 cut(s) 1630
BbsI GAAGAC 2 cut(s) 1184, 1224
BbvI GCAGC 3 cut(s) 661, 1319, 1610
BccI CCATC 4 cut(s) 326, 914, 1145, 1331
BciT130I CCWGG 6 cut(s) 426, 476, 817, 1143, 1220, 1507
BclI TGATCA 1 cut(s) 436
BcnI CCSGG 2 cut(s) 229, 1250
BcoDI GTCTC 4 cut(s) 76, 171, 1693, 1701
BfaI CTAG 3 cut(s) 746, 1008, 1683
BfmI CTRYAG 1 cut(s) 761
BfrI CTTAAG 1 cut(s) 1475
BisI GCNGC 3 cut(s) 675, 1333, 1624
BlnI CCTAGG 1 cut(s) 1007
BlsI GCNGC 3 cut(s) 676, 1334, 1625
Bme1390I CCNGG 8 cut(s) 229, 426, 476, 817, 1143, 1220, 1250, 1507
Bme18I GGWCC 4 cut(s) 573, 1145, 1216, 1499
BmeT110I CYCGRG 1 cut(s) 1495
BmgT120I GGNCC 6 cut(s) 226, 573, 1145, 1216, 1320, 1499
BmiI GGNNCC 6 cut(s) 88, 574, 729, 893, 1281, 1500
BmrFI CCNGG 8 cut(s) 229, 426, 476, 817, 1143, 1220, 1250, 1507
BmsI GCATC 6 cut(s) 270, 673, 682, 1452, 1610, 1635
BoxI GACNNNNGTC 1 cut(s) 811
BpiI GAAGAC 2 cut(s) 1184, 1224
BplI GAGNNNNNCTC 2 cut(s) 1052, 1084
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 2 cut(s) 229, 1250
BsaBI GATNNNNATC 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 1701
BsaJI CCNNGG 7 cut(s) 148, 161, 475, 723, 816, 1007, 1505
BsaXI ACNNNNNCTCC 2 cut(s) 1214, 1244
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 1008
Bse1I ACTGG 1 cut(s) 470
Bse3DI GCAATG 1 cut(s) 28
Bse8I GATNNNNATC 1 cut(s) 102
BseBI CCWGG 6 cut(s) 426, 476, 817, 1143, 1220, 1507
BseDI CCNNGG 7 cut(s) 148, 161, 475, 723, 816, 1007, 1505
BseGI GGATG 4 cut(s) 250, 261, 688, 925
BseJI GATNNNNATC 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 476, 1008
BseMI GCAATG 1 cut(s) 28
BseMII CTCAG 4 cut(s) 507, 609, 751, 1050
BseNI ACTGG 1 cut(s) 470
BseXI GCAGC 3 cut(s) 661, 1319, 1610
BsgI GTGCAG 1 cut(s) 842
Bsh1285I CGRYCG 2 cut(s) 106, 233
BshFI GGCC 3 cut(s) 227, 1012, 1321
BsiEI CGRYCG 2 cut(s) 106, 233
BsiHKCI CYCGRG 1 cut(s) 1495
BsiSI CCGG 2 cut(s) 229, 1249
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 2 cut(s) 476, 1008
BsmAI GTCTC 4 cut(s) 76, 171, 1693, 1701
BsmFI GGGAC 1 cut(s) 472
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 3 cut(s) 227, 1012, 1321
Bso31I GGTCTC 1 cut(s) 1701
BsoBI CYCGRG 1 cut(s) 1495
Bsp1286I GDGCHC 1 cut(s) 1637
Bsp143I GATC 5 cut(s) 103, 436, 727, 740, 1024
Bsp19I CCATGG 1 cut(s) 148
BspACI CCGC 2 cut(s) 1214, 1712
BspANI GGCC 3 cut(s) 227, 1012, 1321
BspCNI CTCAG 4 cut(s) 508, 610, 750, 1051
BspLI GGNNCC 6 cut(s) 88, 574, 729, 893, 1281, 1500
BspPI GGATC 2 cut(s) 722, 735
BspQI GCTCTTC 1 cut(s) 1128
BspTI CTTAAG 1 cut(s) 1475
BspTNI GGTCTC 1 cut(s) 1701
BsrDI GCAATG 1 cut(s) 28
BsrI ACTGG 1 cut(s) 470
BssECI CCNNGG 7 cut(s) 148, 161, 475, 723, 816, 1007, 1505
BssMI GATC 5 cut(s) 103, 436, 727, 740, 1024
BssSI CACGAG 1 cut(s) 1630
BssT1I CCWWGG 4 cut(s) 148, 161, 723, 1007
Bst2BI CACGAG 1 cut(s) 1630
Bst2UI CCWGG 6 cut(s) 426, 476, 817, 1143, 1220, 1507
Bst4CI ACNGT 3 cut(s) 53, 562, 1549
Bst6I CTCTTC 1 cut(s) 1128
BstAFI CTTAAG 1 cut(s) 1475
BstAPI GCANNNNNTGC 1 cut(s) 1471
BstC8I GCNNGC 4 cut(s) 305, 697, 701, 1668
BstDEI CTNAG 8 cut(s) 516, 594, 618, 678, 737, 1059, 1359, 1577
BstDSI CCRYGG 1 cut(s) 148
BstENI CCTNNNNNAGG 1 cut(s) 1006
BstF5I GGATG 4 cut(s) 250, 261, 688, 925
BstKTI GATC 5 cut(s) 106, 439, 730, 743, 1027
BstMAI GTCTC 4 cut(s) 76, 171, 1693, 1701
BstMBI GATC 5 cut(s) 103, 436, 727, 740, 1024
BstMCI CGRYCG 2 cut(s) 106, 233
BstMWI GCNNNNNNNGC 7 cut(s) 683, 692, 757, 1427, 1471, 1580, 1632
BstNI CCWGG 6 cut(s) 426, 476, 817, 1143, 1220, 1507
BstPAI GACNNNNGTC 1 cut(s) 811
BstSCI CCNGG 8 cut(s) 227, 424, 474, 815, 1141, 1218, 1248, 1505
BstSFI CTRYAG 1 cut(s) 761
BstV1I GCAGC 3 cut(s) 661, 1319, 1610
BstV2I GAAGAC 2 cut(s) 1184, 1224
BstX2I RGATCY 1 cut(s) 727
BstXI CCANNNNNNTGG 1 cut(s) 724
BstYI RGATCY 1 cut(s) 727
BsuRI GGCC 3 cut(s) 227, 1012, 1321
BtgI CCRYGG 1 cut(s) 148
BtsCI GGATG 4 cut(s) 250, 261, 688, 925
BtsI GCAGTG 1 cut(s) 830
BtsIMutI CAGTG 2 cut(s) 558, 830
Cac8I GCNNGC 4 cut(s) 305, 697, 701, 1668
Cfr13I GGNCC 6 cut(s) 226, 573, 1145, 1216, 1320, 1499
Csp6I GTAC 3 cut(s) 336, 465, 849
CviAII CATG 4 cut(s) 149, 266, 1306, 1571
CviQI GTAC 3 cut(s) 336, 465, 849
DdeI CTNAG 8 cut(s) 516, 594, 618, 678, 737, 1059, 1359, 1577
DpnI GATC 5 cut(s) 105, 438, 729, 742, 1026
DpnII GATC 5 cut(s) 103, 436, 727, 740, 1024
DraI TTTAAA 2 cut(s) 625, 865
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
Eco130I CCWWGG 4 cut(s) 148, 161, 723, 1007
Eco24I GRGCYC 1 cut(s) 1637
Eco31I GGTCTC 1 cut(s) 1701
Eco47I GGWCC 4 cut(s) 573, 1145, 1216, 1499
Eco57I CTGAAG 1 cut(s) 787
Eco88I CYCGRG 1 cut(s) 1495
EcoNI CCTNNNNNAGG 1 cut(s) 1006
EcoO109I RGGNCCY 2 cut(s) 573, 1499
EcoRII CCWGG 6 cut(s) 424, 474, 815, 1141, 1218, 1505
EcoT14I CCWWGG 4 cut(s) 148, 161, 723, 1007
EcoT38I GRGCYC 1 cut(s) 1637
ErhI CCWWGG 4 cut(s) 148, 161, 723, 1007
FaeI CATG 4 cut(s) 152, 269, 1309, 1574
FalI AAGNNNNNCTT 2 cut(s) 1373, 1405
FaqI GGGAC 1 cut(s) 472
FatI CATG 4 cut(s) 148, 265, 1305, 1570
FauI CCCGC 1 cut(s) 1719
FbaI TGATCA 1 cut(s) 436
Fnu4HI GCNGC 3 cut(s) 675, 1333, 1624
FokI GGATG 4 cut(s) 248, 257, 695, 932
FriOI GRGCYC 1 cut(s) 1637
Fsp4HI GCNGC 3 cut(s) 675, 1333, 1624
FspBI CTAG 3 cut(s) 746, 1008, 1683
GluI GCNGC 3 cut(s) 675, 1333, 1624
GsuI CTGGAG 1 cut(s) 453
HaeIII GGCC 3 cut(s) 227, 1012, 1321
HapII CCGG 2 cut(s) 229, 1249
Hin1II CATG 4 cut(s) 152, 269, 1309, 1574
HincII GTYRAC 1 cut(s) 1525
HindII GTYRAC 1 cut(s) 1525
HindIII AAGCTT 5 cut(s) 305, 549, 629, 1241, 1536
HinfI GANTC 3 cut(s) 195, 897, 1598
HpaII CCGG 2 cut(s) 229, 1249
HphI GGTGA 3 cut(s) 851, 1390, 1673
Hpy166II GTNNAC 2 cut(s) 874, 1525
Hpy188I TCNGA 4 cut(s) 436, 740, 806, 1093
Hpy188III TCNNGA 5 cut(s) 47, 650, 689, 1148, 1630
Hpy8I GTNNAC 2 cut(s) 874, 1525
HpyAV CCTTC 5 cut(s) 586, 1137, 1308, 1404, 1428
HpyCH4III ACNGT 3 cut(s) 53, 562, 1549
HpyCH4V TGCA 9 cut(s) 33, 261, 303, 318, 823, 1293, 1335, 1465, 1626
HpyF10VI GCNNNNNNNGC 7 cut(s) 683, 692, 757, 1427, 1471, 1580, 1632
HpyF3I CTNAG 8 cut(s) 516, 594, 618, 678, 737, 1059, 1359, 1577
Hsp92II CATG 4 cut(s) 152, 269, 1309, 1574
Ksp22I TGATCA 1 cut(s) 436
Kzo9I GATC 5 cut(s) 103, 436, 727, 740, 1024
LguI GCTCTTC 1 cut(s) 1128
LmnI GCTCC 2 cut(s) 86, 1144
Lsp1109I GCAGC 3 cut(s) 661, 1319, 1610
LweI GCATC 6 cut(s) 270, 673, 682, 1452, 1610, 1635
MaeI CTAG 3 cut(s) 746, 1008, 1683
MaeIII GTNAC 1 cut(s) 752
MalI GATC 5 cut(s) 105, 438, 729, 742, 1026
MboI GATC 5 cut(s) 103, 436, 727, 740, 1024
MboII GAAGA 8 cut(s) 536, 793, 1019, 1115, 1189, 1224, 1293, 1559
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 1 cut(s) 1637
MmeI TCCRAC 1 cut(s) 1592
MslI CAYNNNNRTG 1 cut(s) 153
MspCI CTTAAG 1 cut(s) 1475
MspI CCGG 2 cut(s) 229, 1249
MspR9I CCNGG 8 cut(s) 229, 426, 476, 817, 1143, 1220, 1250, 1507
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 6 cut(s) 426, 476, 817, 1143, 1220, 1507
MwoI GCNNNNNNNGC 7 cut(s) 683, 692, 757, 1427, 1471, 1580, 1632
NciI CCSGG 2 cut(s) 229, 1250
NcoI CCATGG 1 cut(s) 148
NdeII GATC 5 cut(s) 103, 436, 727, 740, 1024
NlaIII CATG 4 cut(s) 152, 269, 1309, 1574
NlaIV GGNNCC 6 cut(s) 88, 574, 729, 893, 1281, 1500
NmeAIII GCCGAG 1 cut(s) 1739
NmuCI GTSAC 1 cut(s) 752
PciSI GCTCTTC 1 cut(s) 1128
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 3 cut(s) 195, 897, 1598
PfoI TCCNGGA 2 cut(s) 1218, 1248
PkrI GCNGC 3 cut(s) 676, 1334, 1625
Ple19I CGATCG 1 cut(s) 106
PpuMI RGGWCCY 2 cut(s) 573, 1499
PshAI GACNNNNGTC 1 cut(s) 811
PshBI ATTAAT 2 cut(s) 1404, 1656
PsiI TTATAA 1 cut(s) 1347
Psp5II RGGWCCY 2 cut(s) 573, 1499
Psp6I CCWGG 6 cut(s) 424, 474, 815, 1141, 1218, 1505
PspGI CCWGG 6 cut(s) 424, 474, 815, 1141, 1218, 1505
PspN4I GGNNCC 6 cut(s) 88, 574, 729, 893, 1281, 1500
PspPI GGNCC 6 cut(s) 226, 573, 1145, 1216, 1320, 1499
PspPPI RGGWCCY 2 cut(s) 573, 1499
PsuI RGATCY 1 cut(s) 727
PvuI CGATCG 1 cut(s) 106
RsaI GTAC 3 cut(s) 337, 466, 850
RsaNI GTAC 3 cut(s) 336, 465, 849
RseI CAYNNNNRTG 1 cut(s) 153
SapI GCTCTTC 1 cut(s) 1128
SatI GCNGC 3 cut(s) 675, 1333, 1624
Sau3AI GATC 5 cut(s) 103, 436, 727, 740, 1024
Sau96I GGNCC 6 cut(s) 226, 573, 1145, 1216, 1320, 1499
ScrFI CCNGG 8 cut(s) 229, 426, 476, 817, 1143, 1220, 1250, 1507
SduI GDGCHC 1 cut(s) 1637
SfaNI GCATC 6 cut(s) 270, 673, 682, 1452, 1610, 1635
SfcI CTRYAG 1 cut(s) 761
SinI GGWCC 4 cut(s) 573, 1145, 1216, 1499
SmiMI CAYNNNNRTG 1 cut(s) 153
SmlI CTYRAG 2 cut(s) 278, 1475
SmoI CTYRAG 2 cut(s) 278, 1475
SsiI CCGC 2 cut(s) 1214, 1712
SspI AATATT 2 cut(s) 1036, 1453
SspMI CTAG 3 cut(s) 746, 1008, 1683
StyD4I CCNGG 8 cut(s) 227, 424, 474, 815, 1141, 1218, 1248, 1505
StyI CCWWGG 4 cut(s) 148, 161, 723, 1007
TaaI ACNGT 3 cut(s) 53, 562, 1549
TaqI TCGA 1 cut(s) 233
TatI WGTACW 1 cut(s) 848
TfiI GAWTC 3 cut(s) 195, 897, 1598
TscAI CASTG 2 cut(s) 565, 830
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 3 cut(s) 674, 1332, 1623
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 5 cut(s) 17, 24, 1031, 1552, 1559
TspRI CASTG 2 cut(s) 565, 830
Vha464I CTTAAG 1 cut(s) 1475
VpaK11BI GGWCC 4 cut(s) 573, 1145, 1216, 1499
VspI ATTAAT 2 cut(s) 1404, 1656
XagI CCTNNNNNAGG 1 cut(s) 1006
XapI RAATTY 2 cut(s) 392, 645
XcmI CCANNNNNNNNNTGG 2 cut(s) 983, 1273
XmaJI CCTAGG 1 cut(s) 1007
XspI CTAG 3 cut(s) 746, 1008, 1683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.