Rorug02G0194400

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0004665
Physical Location & Seq
Reverse (-)
18092110 .. 18092634
525 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0194400.1

Sequence Viewer

Length: 525 bp
ATGTTTCCTGATGGGTTTGTCTTCTTCCTTGGTCCGGTTCACTTTCTGATTCCACCAGACTCTGCTGGTGCTGATCTAGGATTGTATAACTCCACCACTCGTTTTGCCTCCCAAAACCAACTCGTAACGGTTGAGTTCGACACTTTCTCGAATCCATGGGATCCACCTGGCCCGCACATTGGGATCAACAACCAGACCATATCCTCAGTTGTTTATGCCAGTTGGGATCCTATCCTCCACAGTGGGAAAGAGGGCCGAGCACATATTACTTATAATGCTATCACCAACAACCTCAGTGTGTTTTGGACCTATGACGGAGGCCCAAACCCTGAACTTAATAGCTATGTGGCAAACTCTTCCCTTTTCATCCTTATTGACCTAAGAAATGCTCTCCCCGAGTGGGTTAGAATTGGATTTTCATCTGCTACAGGCCTTTTCTGGGAGCGCCATTTTATAAGTTCATGGGAATTCAGTTCGAATTTGGATACTAAAGATATCGTAAAAAGAAAAAGAAGAAGAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

174

Amino Acids

19.6

Weight (kDa)

5.9

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 4 - 168 1.8e-38 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 273, 455
AciI CCGC 1 cut(s) 173
AclWI GGATC 5 cut(s) 155, 168, 191, 221, 234
AcsI RAATTY 2 cut(s) 467, 478
AfiI CCNNNNNNNGG 4 cut(s) 34, 179, 400, 439
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 1 cut(s) 342
AluI AGCT 1 cut(s) 342
Alw21I GWGCWC 1 cut(s) 262
AlwI GGATC 5 cut(s) 155, 168, 191, 221, 234
AlwNI CAGNNNCTG 1 cut(s) 62
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 4 cut(s) 169, 253, 319, 430
ApoI RAATTY 2 cut(s) 467, 478
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 5 cut(s) 32, 170, 253, 306, 320
AsuHPI GGTGA 1 cut(s) 274
AsuII TTCGAA 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 2 cut(s) 32, 306
BamHI GGATCC 2 cut(s) 160, 226
BbsI GAAGAC 1 cut(s) 13
Bbv12I GWGCWC 1 cut(s) 262
BccI CCATC 1 cut(s) 5
BciT130I CCWGG 1 cut(s) 168
BciVI GTATCC 1 cut(s) 478
BfaI CTAG 1 cut(s) 77
BfmI CTRYAG 1 cut(s) 426
BfoI RGCGCY 1 cut(s) 448
BfuI GTATCC 1 cut(s) 478
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 2 cut(s) 32, 306
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 5 cut(s) 32, 170, 253, 306, 320
BmiI GGNNCC 2 cut(s) 162, 228
BmrFI CCNGG 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 13
Bpu14I TTCGAA 1 cut(s) 476
BsaBI GATNNNNATC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 28, 155
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 179, 400, 439
Bse1I ACTGG 1 cut(s) 219
Bse8I GATNNNNATC 1 cut(s) 418
BseBI CCWGG 1 cut(s) 168
BseDI CCNNGG 2 cut(s) 28, 155
BseGI GGATG 1 cut(s) 366
BseJI GATNNNNATC 1 cut(s) 418
BseLI CCNNNNNNNGG 4 cut(s) 34, 179, 400, 439
BseMII CTCAG 2 cut(s) 219, 307
BseNI ACTGG 1 cut(s) 219
BshFI GGCC 4 cut(s) 171, 255, 321, 432
BsiHKAI GWGCWC 1 cut(s) 262
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 4 cut(s) 34, 179, 400, 439
BsnI GGCC 4 cut(s) 171, 255, 321, 432
BsoBI CYCGRG 1 cut(s) 395
Bsp119I TTCGAA 1 cut(s) 476
Bsp1286I GDGCHC 1 cut(s) 262
Bsp143I GATC 4 cut(s) 73, 160, 183, 226
Bsp19I CCATGG 1 cut(s) 155
BspACI CCGC 1 cut(s) 173
BspANI GGCC 4 cut(s) 171, 255, 321, 432
BspCNI CTCAG 2 cut(s) 218, 306
BspLI GGNNCC 2 cut(s) 162, 228
BspPI GGATC 5 cut(s) 155, 168, 191, 221, 234
BspT104I TTCGAA 1 cut(s) 476
BsrI ACTGG 1 cut(s) 219
BssECI CCNNGG 2 cut(s) 28, 155
BssMI GATC 4 cut(s) 73, 160, 183, 226
BssT1I CCWWGG 2 cut(s) 28, 155
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 130, 242
Bst6I CTCTTC 2 cut(s) 361, 511
BstBI TTCGAA 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 173
BstDEI CTNAG 3 cut(s) 205, 293, 380
BstDSI CCRYGG 1 cut(s) 155
BstF5I GGATG 1 cut(s) 366
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 4 cut(s) 76, 163, 186, 229
BstMBI GATC 4 cut(s) 73, 160, 183, 226
BstNI CCWGG 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 13
BstX2I RGATCY 2 cut(s) 160, 226
BstYI RGATCY 2 cut(s) 160, 226
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 4 cut(s) 171, 255, 321, 432
BtgI CCRYGG 1 cut(s) 155
BtsCI GGATG 1 cut(s) 366
BtsIMutI CAGTG 2 cut(s) 247, 301
Cac8I GCNNGC 1 cut(s) 173
CaiI CAGNNNCTG 1 cut(s) 62
CfoI GCGC 1 cut(s) 447
Cfr13I GGNCC 5 cut(s) 32, 170, 253, 306, 320
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 2 cut(s) 156, 462
CviJI RGCY 5 cut(s) 171, 255, 321, 342, 432
CviKI_1 RGCY 5 cut(s) 171, 255, 321, 342, 432
DdeI CTNAG 3 cut(s) 205, 293, 380
DpnI GATC 4 cut(s) 75, 162, 185, 228
DpnII GATC 4 cut(s) 73, 160, 183, 226
Eam1104I CTCTTC 2 cut(s) 361, 511
EarI CTCTTC 2 cut(s) 361, 511
Eco130I CCWWGG 2 cut(s) 28, 155
Eco147I AGGCCT 1 cut(s) 432
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 2 cut(s) 32, 306
Eco88I CYCGRG 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 467
EcoRII CCWGG 1 cut(s) 166
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 28, 155
ErhI CCWWGG 2 cut(s) 28, 155
FaeI CATG 2 cut(s) 159, 465
FatI CATG 2 cut(s) 155, 461
FauI CCCGC 1 cut(s) 180
FokI GGATG 1 cut(s) 353
FspBI CTAG 1 cut(s) 77
GlaI GCGC 1 cut(s) 446
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 4 cut(s) 171, 255, 321, 432
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 447
Hin1II CATG 2 cut(s) 159, 465
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HinfI GANTC 3 cut(s) 49, 59, 151
HpaII CCGG 1 cut(s) 35
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 1 cut(s) 40
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 2 cut(s) 8, 148
Hpy8I GTNNAC 1 cut(s) 40
HpyCH4III ACNGT 2 cut(s) 130, 242
HpyF3I CTNAG 3 cut(s) 205, 293, 380
Hsp92II CATG 2 cut(s) 159, 465
HspAI GCGC 1 cut(s) 445
Kzo9I GATC 4 cut(s) 73, 160, 183, 226
LmnI GCTCC 1 cut(s) 442
MaeI CTAG 1 cut(s) 77
MaeIII GTNAC 1 cut(s) 124
MalI GATC 4 cut(s) 75, 162, 185, 228
MboI GATC 4 cut(s) 73, 160, 183, 226
MboII GAAGA 4 cut(s) 13, 16, 348, 525
MflI RGATCY 2 cut(s) 160, 226
MhlI GDGCHC 1 cut(s) 262
MluCI AATT 3 cut(s) 408, 467, 478
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 7 cut(s) 118, 214, 244, 245, 302, 311, 512
MseI TTAA 1 cut(s) 336
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
NcoI CCATGG 1 cut(s) 155
NdeII GATC 4 cut(s) 73, 160, 183, 226
NlaIII CATG 2 cut(s) 159, 465
NlaIV GGNNCC 2 cut(s) 162, 228
NmeAIII GCCGAG 1 cut(s) 281
NspV TTCGAA 1 cut(s) 476
PceI AGGCCT 1 cut(s) 432
PfeI GAWTC 2 cut(s) 49, 151
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PsiI TTATAA 2 cut(s) 273, 455
Psp6I CCWGG 1 cut(s) 166
PspGI CCWGG 1 cut(s) 166
PspN4I GGNNCC 2 cut(s) 162, 228
PspPI GGNCC 5 cut(s) 32, 170, 253, 306, 320
PstNI CAGNNNCTG 1 cut(s) 62
PsuI RGATCY 2 cut(s) 160, 226
SaqAI TTAA 1 cut(s) 336
Sau3AI GATC 4 cut(s) 73, 160, 183, 226
Sau96I GGNCC 5 cut(s) 32, 170, 253, 306, 320
SchI GAGTC 1 cut(s) 53
ScrFI CCNGG 1 cut(s) 168
SduI GDGCHC 1 cut(s) 262
SetI ASST 5 cut(s) 169, 294, 311, 344, 381
SfcI CTRYAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 476
SinI GGWCC 2 cut(s) 32, 306
Sse9I AATT 3 cut(s) 408, 467, 478
SseBI AGGCCT 1 cut(s) 432
SsiI CCGC 1 cut(s) 173
SspMI CTAG 1 cut(s) 77
StuI AGGCCT 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 166
StyI CCWWGG 2 cut(s) 28, 155
TaaI ACNGT 2 cut(s) 130, 242
TaqI TCGA 3 cut(s) 138, 149, 476
TasI AATT 3 cut(s) 408, 467, 478
TfiI GAWTC 2 cut(s) 49, 151
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TscAI CASTG 2 cut(s) 247, 301
TspDTI ATGAA 3 cut(s) 355, 408, 450
TspGWI ACGGA 1 cut(s) 330
TspRI CASTG 2 cut(s) 247, 301
VpaK11BI GGWCC 2 cut(s) 32, 306
XapI RAATTY 2 cut(s) 467, 478
XspI CTAG 1 cut(s) 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.