MD13G1122000.v1.1

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004665
Physical Location & Seq
Reverse (-)
8983382 .. 8989298
5917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1122000.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGGCTGCTCTCCCTCTCGGCAAGCTCACCATCCTCGTGGGCGCAGGCATTCTTGGTTCAGTTCTTGCAAAAGAAGGACGTGTATCTGATGTTGTCTCTGGTGCTTTCAAGATTGCATGGAAACAAATTATACGAAGTGATAGTACTCCGGCGGCTAAGAAACCACACAACGACCATCTGATGGCTCAGATGAACAACCTACGGAAGGAGCTGGAGATGATATCATCAAGTCCTCGGCAACGAGTTACAATTATAACTATGGGTCGAACAGGTACTAGCAAATATGGTATAATTATTGTTGCAGTAGCGGCGGGATATGGCTACTTGTGGTGGAAGGGGTGGAAGCTTCCTGATATGATGTTTGCAACAAGACGTAGCTTATCTGATGCTTCCAATTCTGTTGCTAAACAGCTTGAGAGTCTGTTTACATCAATTTCGAGCACTCGGAGGAAATTATCTTCACAACTTGATCGTGTGGAATGTAGTTTGGATGAAAGTATTGAAAATACTGCTAGGACGCAACAGGAGGTTATCACACTACAAGGAAAGACGGACACCATTAGCAGGGATTTTAAAAAAGTTCATCATGCAGTGCTGACTCTGGAAACAAAAATTAATAGAATCGAAGGGAAGCAGGGTGAGACTGCTGATGGAGTCTGGAAGTTGTGCGACTATGCCCTGAACCTGGAAAAGGGCAGAAACGCAGAACGCATTCAGGCATCACCATCCAGCTTTTCCAGGTCAGTTCTTGAACTACCACCAGACTCCCCCTCGTCTTTGGTGACACCACTGAAGTCCCCGAGGCCAGCACTTGAGCTACCCCCAACGTCACCCGCAAGGGAGAATAGTGGACTCTTGGAAGTGGTTGAGTCCTCCTCAAATCGTTCTGAGGCTTCCAATGGGATTCGTGCCCAAGAAGGCACAAACAAAGCGGCTTCCAGTTCTGGTTGGTTCGGGTTCGGGTTTCCAACCTTCAATGGTTCGGCGCTCCAGAGGACGCGAAGTGCTACAACTGGAATGGTGCAACAGAAACGATCAACCAGTCAACCATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

352

Amino Acids

38.21

Weight (kDa)

9.87

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 92 - 212 1.1e-41 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 181
AccII CGCG 1 cut(s) 1000
AciI CCGC 5 cut(s) 152, 308, 311, 834, 932
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 2 cut(s) 145, 274
AfiI CCNNNNNNNGG 5 cut(s) 181, 205, 564, 685, 691
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 4 cut(s) 109, 503, 752, 976
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 2 cut(s) 684, 737
AluBI AGCT 7 cut(s) 25, 211, 346, 378, 412, 732, 817
AluI AGCT 7 cut(s) 25, 211, 346, 378, 412, 732, 817
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 2 cut(s) 100, 635
Ama87I CYCGRG 1 cut(s) 799
AoxI GGCC 1 cut(s) 803
ApeKI GCWGC 1 cut(s) 5
AseI ATTAAT 1 cut(s) 615
Asp700I GAANNNNTTC 1 cut(s) 711
AspLEI GCGC 2 cut(s) 44, 988
AsuHPI GGTGA 5 cut(s) 19, 650, 714, 793, 822
AvaI CYCGRG 1 cut(s) 799
BaeGI GKGCMC 1 cut(s) 913
BarI GAAGNNNNNNTAC 2 cut(s) 127, 159
BauI CACGAG 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 443
BccI CCATC 5 cut(s) 38, 175, 183, 644, 733
BciT130I CCWGG 2 cut(s) 686, 739
BcoDI GTCTC 2 cut(s) 100, 635
BfaI CTAG 2 cut(s) 276, 513
BfoI RGCGCY 1 cut(s) 989
BisI GCNGC 4 cut(s) 6, 153, 309, 933
BlsI GCNGC 4 cut(s) 7, 154, 310, 934
BmcAI AGTACT 1 cut(s) 145
Bme1390I CCNGG 2 cut(s) 686, 739
BmeT110I CYCGRG 1 cut(s) 799
BmgBI CACGTC 1 cut(s) 80
BmrFI CCNGG 2 cut(s) 686, 739
BmsI GCATC 2 cut(s) 376, 728
BplI GAGNNNNNCTC 2 cut(s) 860, 892
BpmI CTGGAG 2 cut(s) 233, 974
BpuEI CTTGAG 2 cut(s) 434, 833
BsaJI CCNNGG 2 cut(s) 233, 800
Bsc4I CCNNNNNNNGG 5 cut(s) 181, 205, 564, 685, 691
Bse1I ACTGG 3 cut(s) 939, 1018, 1041
BseBI CCWGG 2 cut(s) 686, 739
BseDI CCNNGG 2 cut(s) 233, 800
BseGI GGATG 3 cut(s) 30, 496, 725
BseLI CCNNNNNNNGG 5 cut(s) 181, 205, 564, 685, 691
BseMII CTCAG 2 cut(s) 200, 879
BseNI ACTGG 3 cut(s) 939, 1018, 1041
BseRI GAGGAG 1 cut(s) 865
BseSI GKGCMC 1 cut(s) 913
Bsh1236I CGCG 1 cut(s) 1000
BshFI GGCC 1 cut(s) 805
BsiHKAI GWGCWC 1 cut(s) 443
BsiHKCI CYCGRG 1 cut(s) 799
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 781
BslI CCNNNNNNNGG 5 cut(s) 181, 205, 564, 685, 691
BsmAI GTCTC 2 cut(s) 100, 635
BsmFI GGGAC 1 cut(s) 781
BsmI GAATGC 2 cut(s) 48, 711
BsnI GGCC 1 cut(s) 805
BsoBI CYCGRG 1 cut(s) 799
Bsp1286I GDGCHC 2 cut(s) 443, 913
Bsp143I GATC 2 cut(s) 469, 1034
BspACI CCGC 5 cut(s) 152, 308, 311, 834, 932
BspANI GGCC 1 cut(s) 805
BspCNI CTCAG 2 cut(s) 199, 880
BspFNI CGCG 1 cut(s) 1000
BsrI ACTGG 3 cut(s) 939, 1018, 1041
BssECI CCNNGG 2 cut(s) 233, 800
BssMI GATC 2 cut(s) 469, 1034
BssSI CACGAG 1 cut(s) 35
Bst2BI CACGAG 1 cut(s) 35
Bst2UI CCWGG 2 cut(s) 686, 739
BstC8I GCNNGC 3 cut(s) 23, 46, 807
BstDEI CTNAG 3 cut(s) 156, 186, 888
BstENI CCTNNNNNAGG 2 cut(s) 203, 689
BstF5I GGATG 3 cut(s) 30, 496, 725
BstFNI CGCG 1 cut(s) 1000
BstH2I RGCGCY 1 cut(s) 989
BstHHI GCGC 2 cut(s) 44, 988
BstKTI GATC 2 cut(s) 472, 1037
BstMAI GTCTC 2 cut(s) 100, 635
BstMBI GATC 2 cut(s) 469, 1034
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 2 cut(s) 686, 739
BstSCI CCNGG 2 cut(s) 684, 737
BstSLI GKGCMC 1 cut(s) 913
BstUI CGCG 1 cut(s) 1000
BstXI CCANNNNNNTGG 1 cut(s) 37
BsuRI GGCC 1 cut(s) 805
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 3 cut(s) 30, 496, 725
BtsI GCAGTG 1 cut(s) 597
BtsIMutI CAGTG 2 cut(s) 597, 788
Cac8I GCNNGC 3 cut(s) 23, 46, 807
CfoI GCGC 2 cut(s) 44, 988
CseI GACGC 2 cut(s) 526, 1006
Csp6I GTAC 2 cut(s) 144, 273
CviAII CATG 2 cut(s) 117, 587
CviQI GTAC 2 cut(s) 144, 273
DdeI CTNAG 3 cut(s) 156, 186, 888
DpnI GATC 2 cut(s) 471, 1036
DpnII GATC 2 cut(s) 469, 1034
DraI TTTAAA 1 cut(s) 574
Eco32I GATATC 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 799
EcoNI CCTNNNNNAGG 2 cut(s) 203, 689
EcoRII CCWGG 2 cut(s) 684, 737
EcoRV GATATC 1 cut(s) 222
FaeI CATG 2 cut(s) 120, 590
FaqI GGGAC 1 cut(s) 781
FatI CATG 2 cut(s) 116, 586
FauI CCCGC 2 cut(s) 304, 841
Fnu4HI GCNGC 4 cut(s) 6, 153, 309, 933
FokI GGATG 3 cut(s) 17, 503, 712
Fsp4HI GCNGC 4 cut(s) 6, 153, 309, 933
FspBI CTAG 2 cut(s) 276, 513
GlaI GCGC 2 cut(s) 43, 987
GluI GCNGC 4 cut(s) 6, 153, 309, 933
GsuI CTGGAG 2 cut(s) 233, 974
HaeII RGCGCY 1 cut(s) 989
HaeIII GGCC 1 cut(s) 805
HapII CCGG 1 cut(s) 149
HgaI GACGC 2 cut(s) 526, 1006
HhaI GCGC 2 cut(s) 44, 988
Hin1II CATG 2 cut(s) 120, 590
Hin6I GCGC 2 cut(s) 42, 986
HinP1I GCGC 2 cut(s) 42, 986
HincII GTYRAC 1 cut(s) 1046
HindII GTYRAC 1 cut(s) 1046
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 8 cut(s) 418, 598, 621, 654, 764, 852, 869, 904
HpaII CCGG 1 cut(s) 149
HphI GGTGA 5 cut(s) 19, 650, 714, 793, 822
Hpy166II GTNNAC 3 cut(s) 426, 851, 1046
Hpy188I TCNGA 6 cut(s) 88, 180, 189, 385, 447, 889
Hpy188III TCNNGA 6 cut(s) 109, 350, 602, 658, 749, 991
Hpy8I GTNNAC 3 cut(s) 426, 851, 1046
HpyAV CCTTC 6 cut(s) 68, 199, 328, 620, 911, 982
HpyCH4IV ACGT 3 cut(s) 79, 373, 827
HpyCH4V TGCA 6 cut(s) 68, 116, 302, 365, 590, 1024
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 3 cut(s) 156, 186, 888
HpySE526I ACGT 3 cut(s) 79, 373, 827
Hsp92II CATG 2 cut(s) 120, 590
HspAI GCGC 2 cut(s) 42, 986
Kzo9I GATC 2 cut(s) 469, 1034
LmnI GCTCC 2 cut(s) 208, 993
LweI GCATC 2 cut(s) 376, 728
MaeI CTAG 2 cut(s) 276, 513
MaeII ACGT 3 cut(s) 79, 373, 827
MaeIII GTNAC 3 cut(s) 244, 781, 828
MalI GATC 2 cut(s) 471, 1036
MboI GATC 2 cut(s) 469, 1034
MboII GAAGA 1 cut(s) 450
MhlI GDGCHC 2 cut(s) 443, 913
MluCI AATT 7 cut(s) 126, 249, 291, 394, 432, 452, 612
MlyI GAGTC 6 cut(s) 427, 592, 663, 758, 846, 878
MmeI TCCRAC 1 cut(s) 992
MroXI GAANNNNTTC 1 cut(s) 711
MseI TTAA 2 cut(s) 573, 615
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 2 cut(s) 686, 739
Mva1269I GAATGC 2 cut(s) 48, 711
MvaI CCWGG 2 cut(s) 686, 739
MvnI CGCG 1 cut(s) 1000
MwoI GCNNNNNNNGC 1 cut(s) 308
NdeII GATC 2 cut(s) 469, 1034
NlaIII CATG 2 cut(s) 120, 590
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 2 cut(s) 781, 828
PctI GAATGC 2 cut(s) 48, 711
PdmI GAANNNNTTC 1 cut(s) 711
PfeI GAWTC 2 cut(s) 621, 904
PflMI CCANNNNNTGG 1 cut(s) 181
PkrI GCNGC 4 cut(s) 7, 154, 310, 934
PleI GAGTC 6 cut(s) 426, 592, 662, 758, 846, 877
PpsI GAGTC 6 cut(s) 426, 592, 662, 758, 846, 877
PshBI ATTAAT 1 cut(s) 615
PsiI TTATAA 1 cut(s) 254
Psp6I CCWGG 2 cut(s) 684, 737
PspGI CCWGG 2 cut(s) 684, 737
RsaI GTAC 2 cut(s) 145, 274
RsaNI GTAC 2 cut(s) 144, 273
RseI CAYNNNNRTG 1 cut(s) 35
SaqAI TTAA 2 cut(s) 573, 615
SatI GCNGC 4 cut(s) 6, 153, 309, 933
Sau3AI GATC 2 cut(s) 469, 1034
ScaI AGTACT 1 cut(s) 145
SchI GAGTC 6 cut(s) 427, 592, 663, 758, 846, 878
ScrFI CCNGG 2 cut(s) 686, 739
SduI GDGCHC 2 cut(s) 443, 913
SfaNI GCATC 2 cut(s) 376, 728
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 2 cut(s) 413, 812
SmoI CTYRAG 2 cut(s) 413, 812
Sse9I AATT 7 cut(s) 126, 249, 291, 394, 432, 452, 612
SsiI CCGC 5 cut(s) 152, 308, 311, 834, 932
SspMI CTAG 2 cut(s) 276, 513
StyD4I CCNGG 2 cut(s) 684, 737
TaiI ACGT 3 cut(s) 82, 376, 830
TaqI TCGA 3 cut(s) 265, 437, 624
TasI AATT 7 cut(s) 126, 249, 291, 394, 432, 452, 612
TatI WGTACW 1 cut(s) 143
TauI GCSGC 3 cut(s) 155, 311, 935
TfiI GAWTC 2 cut(s) 621, 904
Tru1I TTAA 2 cut(s) 573, 615
Tru9I TTAA 2 cut(s) 573, 615
TscAI CASTG 2 cut(s) 597, 795
TseFI GTSAC 2 cut(s) 781, 828
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 781, 828
TspDTI ATGAA 3 cut(s) 206, 507, 572
TspGWI ACGGA 2 cut(s) 217, 566
TspRI CASTG 2 cut(s) 597, 795
Van91I CCANNNNNTGG 1 cut(s) 181
VspI ATTAAT 1 cut(s) 615
XagI CCTNNNNNAGG 2 cut(s) 203, 689
XmnI GAANNNNTTC 1 cut(s) 711
XspI CTAG 2 cut(s) 276, 513
ZrmI AGTACT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.