FvH4_4g23490

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0004665
Physical Location & Seq
Forward (+)
25948081 .. 25954224
6144 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23490.t2

Sequence Viewer

Length: 759 bp
ATGGCTCTGCCTCTCGTCAAGGTCGGGGTTCTCGTCGGTGCAGGCATTCTTGGTTCATACCTTGCTCAGGAAGGACGCATATCTGATGTGGTGTCTGGTGCATTTAAGATTGCTTGGAAGCAAATACGAGATGATAAAACTAAAGCTCCCTCGGTTAAGAAACCATCCAATGATGCTTTGATGGCTCAGATGAACAAAATACGGCAGGAGCTGCAGTCATATTCGTCAACTGGACCAATTACAATAGTAACATGCAGTCGAACAGGTGCTAGCAGATATTGTTTAATTATTGTTGCAGTAGTGGCAGGATATGGCTACTTTTGGTGGAAGGGGTGGAAACTTCCTGATATGATGTTTGCAACCAGACGTAGCTTAGCTGATGCTGGGAAGTCTTTTTCTAAACAGATTGACGGTGTCTTTTCATCAATTTCAGTCACCAGGCGACAATTATCTTCGAATTTGGATGGTGTGGACCGTAATTTAGATGAGTGCATTGAAACAACTGTTAGGACACAACAGGAGGTTATTGAACTACAAGGAAAAACAGATTCCATCAGCACAAATTTTAGAGAAGTTCAACATGCAGTGCTTACTCTGGAAACTAAGATTAACAGAATAGAAGGCAAGCAGGATTCAACTAATGACGGAGTGTGGAAGTTGTGTGACTATGCCAAGAGATTGGAAAATACTCAAAATAGCAGAACCACAGAACGTATTCAGGCATGCATCATCGTCCAACTCTTCCGGGCGATCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

253

Amino Acids

28.03

Weight (kDa)

9.38

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 94 - 210 1.2e-39 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 457, 562
AfiI CCNNNNNNNGG 1 cut(s) 67
AgsI TTSAA 4 cut(s) 497, 530, 578, 636
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 4 cut(s) 146, 211, 372, 377
AluI AGCT 4 cut(s) 146, 211, 372, 377
AlwNI CAGNNNCTG 1 cut(s) 211
ApeKI GCWGC 1 cut(s) 211
ApoI RAATTY 2 cut(s) 457, 562
Asp700I GAANNNNTTC 1 cut(s) 714
AspS9I GGNCC 2 cut(s) 233, 472
AsuC2I CCSGG 1 cut(s) 746
AsuHPI GGTGA 1 cut(s) 427
AsuII TTCGAA 1 cut(s) 455
AsuNHI GCTAGC 1 cut(s) 269
AvaII GGWCC 2 cut(s) 233, 472
BbvI GCAGC 1 cut(s) 198
BccI CCATC 4 cut(s) 172, 175, 458, 560
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 439
BcnI CCSGG 1 cut(s) 746
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 212
BisI GCNGC 1 cut(s) 212
BlpI GCTNAGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 213
Bme1390I CCNGG 2 cut(s) 439, 746
Bme18I GGWCC 2 cut(s) 233, 472
BmgT120I GGNCC 2 cut(s) 233, 472
BmrFI CCNGG 2 cut(s) 439, 746
BmsI GCATC 3 cut(s) 163, 370, 735
BmtI GCTAGC 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 66
Bpu1102I GCTNAGC 1 cut(s) 373
Bpu14I TTCGAA 1 cut(s) 455
BpuMI CCSGG 1 cut(s) 746
BsaJI CCNNGG 1 cut(s) 150
BsaXI ACNNNNNCTCC 4 cut(s) 130, 160, 200, 230
Bsc4I CCNNNNNNNGG 1 cut(s) 67
Bse1I ACTGG 1 cut(s) 235
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 2 cut(s) 164, 469
BseLI CCNNNNNNNGG 1 cut(s) 67
BseMII CTCAG 2 cut(s) 80, 200
BseNI ACTGG 1 cut(s) 235
BseXI GCAGC 1 cut(s) 198
BseYI CCCAGC 1 cut(s) 383
BsgI GTGCAG 1 cut(s) 60
BsiSI CCGG 1 cut(s) 745
BslI CCNNNNNNNGG 1 cut(s) 67
BsmI GAATGC 1 cut(s) 45
Bsp119I TTCGAA 1 cut(s) 455
Bsp143I GATC 1 cut(s) 750
Bsp1720I GCTNAGC 1 cut(s) 373
BspCNI CTCAG 2 cut(s) 79, 199
BspMAI CTGCAG 1 cut(s) 216
BspOI GCTAGC 1 cut(s) 273
BspT104I TTCGAA 1 cut(s) 455
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 1 cut(s) 750
Bst2UI CCWGG 1 cut(s) 439
Bst4CI ACNGT 3 cut(s) 413, 476, 505
Bst6I CTCTTC 1 cut(s) 746
BstAPI GCANNNNNTGC 1 cut(s) 211
BstBI TTCGAA 1 cut(s) 455
BstC8I GCNNGC 4 cut(s) 43, 271, 626, 724
BstDEI CTNAG 4 cut(s) 66, 186, 373, 603
BstENI CCTNNNNNAGG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 164, 469
BstKTI GATC 1 cut(s) 753
BstMBI GATC 1 cut(s) 750
BstMWI GCNNNNNNNGC 3 cut(s) 182, 211, 302
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 3 cut(s) 255, 584, 726
BstSCI CCNGG 2 cut(s) 437, 744
BstSFI CTRYAG 1 cut(s) 212
BstV1I GCAGC 1 cut(s) 198
BstXI CCANNNNNNTGG 1 cut(s) 679
BtsCI GGATG 2 cut(s) 164, 469
BtsI GCAGTG 1 cut(s) 591
BtsIMutI CAGTG 1 cut(s) 591
Cac8I GCNNGC 4 cut(s) 43, 271, 626, 724
CaiI CAGNNNCTG 1 cut(s) 211
Cfr13I GGNCC 2 cut(s) 233, 472
CseI GACGC 1 cut(s) 84
CviAII CATG 3 cut(s) 252, 581, 723
CviJI RGCY 7 cut(s) 5, 146, 185, 211, 315, 372, 377
CviKI_1 RGCY 7 cut(s) 5, 146, 185, 211, 315, 372, 377
DdeI CTNAG 4 cut(s) 66, 186, 373, 603
DpnI GATC 1 cut(s) 752
DpnII GATC 1 cut(s) 750
Eam1104I CTCTTC 1 cut(s) 746
EarI CTCTTC 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 233, 472
EcoNI CCTNNNNNAGG 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 437
EcoT22I ATGCAT 1 cut(s) 728
FaeI CATG 3 cut(s) 255, 584, 726
FaiI YATR 9 cut(s) 58, 80, 220, 253, 312, 350, 582, 669, 724
FatI CATG 3 cut(s) 251, 580, 722
Fnu4HI GCNGC 1 cut(s) 212
FokI GGATG 2 cut(s) 151, 476
Fsp4HI GCNGC 1 cut(s) 212
FspBI CTAG 1 cut(s) 270
GluI GCNGC 1 cut(s) 212
GsaI CCCAGC 1 cut(s) 387
HapII CCGG 1 cut(s) 745
HgaI GACGC 1 cut(s) 84
Hin1II CATG 3 cut(s) 255, 584, 726
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HinfI GANTC 2 cut(s) 548, 632
HpaII CCGG 1 cut(s) 745
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 2 cut(s) 228, 472
Hpy188I TCNGA 2 cut(s) 85, 189
Hpy188III TCNNGA 4 cut(s) 68, 344, 596, 756
Hpy8I GTNNAC 2 cut(s) 228, 472
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 3 cut(s) 65, 322, 614
HpyCH4III ACNGT 3 cut(s) 413, 476, 505
HpyCH4IV ACGT 2 cut(s) 367, 712
HpyCH4V TGCA 9 cut(s) 41, 101, 214, 255, 296, 359, 492, 584, 726
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 211, 302
HpyF3I CTNAG 4 cut(s) 66, 186, 373, 603
HpySE526I ACGT 2 cut(s) 367, 712
Hsp92II CATG 3 cut(s) 255, 584, 726
Kzo9I GATC 1 cut(s) 750
LmnI GCTCC 2 cut(s) 151, 208
Lsp1109I GCAGC 1 cut(s) 198
LweI GCATC 3 cut(s) 163, 370, 735
MaeI CTAG 1 cut(s) 270
MaeII ACGT 2 cut(s) 367, 712
MaeIII GTNAC 3 cut(s) 247, 433, 662
MalI GATC 1 cut(s) 752
MboI GATC 1 cut(s) 750
MboII GAAGA 2 cut(s) 444, 733
MluCI AATT 7 cut(s) 237, 285, 426, 446, 457, 478, 562
MnlI CCTC 3 cut(s) 21, 160, 514
Mph1103I ATGCAT 1 cut(s) 728
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 4 cut(s) 105, 156, 284, 609
MspI CCGG 1 cut(s) 745
MspR9I CCNGG 2 cut(s) 439, 746
Mva1269I GAATGC 1 cut(s) 45
MvaI CCWGG 1 cut(s) 439
MwoI GCNNNNNNNGC 3 cut(s) 182, 211, 302
NciI CCSGG 1 cut(s) 746
NdeII GATC 1 cut(s) 750
NheI GCTAGC 1 cut(s) 269
NlaIII CATG 3 cut(s) 255, 584, 726
NmuCI GTSAC 2 cut(s) 433, 662
NsiI ATGCAT 1 cut(s) 728
NspI RCATGY 3 cut(s) 255, 584, 726
NspV TTCGAA 1 cut(s) 455
PaeI GCATGC 1 cut(s) 726
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 45
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 2 cut(s) 548, 632
PflFI GACNNNGTC 1 cut(s) 413
PkrI GCNGC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 437
PspFI CCCAGC 1 cut(s) 383
PspGI CCWGG 1 cut(s) 437
PspPI GGNCC 2 cut(s) 233, 472
PstI CTGCAG 1 cut(s) 216
PstNI CAGNNNCTG 1 cut(s) 211
PsyI GACNNNGTC 1 cut(s) 413
SaqAI TTAA 4 cut(s) 105, 156, 284, 609
SatI GCNGC 1 cut(s) 212
Sau3AI GATC 1 cut(s) 750
Sau96I GGNCC 2 cut(s) 233, 472
ScrFI CCNGG 2 cut(s) 439, 746
SfaNI GCATC 3 cut(s) 163, 370, 735
SfcI CTRYAG 1 cut(s) 212
SfuI TTCGAA 1 cut(s) 455
SinI GGWCC 2 cut(s) 233, 472
SphI GCATGC 1 cut(s) 726
Sse9I AATT 7 cut(s) 237, 285, 426, 446, 457, 478, 562
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 2 cut(s) 437, 744
TaaI ACNGT 3 cut(s) 413, 476, 505
TaiI ACGT 2 cut(s) 370, 715
TaqI TCGA 2 cut(s) 259, 455
TasI AATT 7 cut(s) 237, 285, 426, 446, 457, 478, 562
TfiI GAWTC 2 cut(s) 548, 632
Tru1I TTAA 4 cut(s) 105, 156, 284, 609
Tru9I TTAA 4 cut(s) 105, 156, 284, 609
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 2 cut(s) 433, 662
TseI GCWGC 1 cut(s) 211
Tsp45I GTSAC 2 cut(s) 433, 662
TspDTI ATGAA 3 cut(s) 45, 206, 411
TspGWI ACGGA 1 cut(s) 660
TspRI CASTG 1 cut(s) 591
Tth111I GACNNNGTC 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 233, 472
XagI CCTNNNNNAGG 1 cut(s) 65
XapI RAATTY 2 cut(s) 457, 562
XceI RCATGY 3 cut(s) 255, 584, 726
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 1 cut(s) 270
Zsp2I ATGCAT 1 cut(s) 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.