MD16G1122300.v1.1

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004665
Physical Location & Seq
Reverse (-)
8800639 .. 8806778
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1122300.v1.1.491

Sequence Viewer

Length: 1032 bp
ATGGCTGCTCTCCCTCTAGGCAAGCTTACCATTCTCGTCGGCGCAGGCATTCTCGGTTCAGTTCTTGCAAAAGAAGGACGTGTATCTGATGTTGTCTCTGGTGCTTTCAAGATTGCTTGGAAACAAATTAGACGAAGTGATAATACTCCGGCGGTTAATAAACCACACAATGACTCTCTGATGGCTCAGATGAACAACCTACGGAAGGAGCTGGAGATAATAGCATCAAGTCGACCACCAGTTACAATTATAACTATGAGTCGAACAGGTACTAGCAAATATGGTATAATTATTGTTGTAGTAGCCGCTGGATATGGCTACTTGTGGTGGAAGGGGTGGAAGCTTCCTGACATGATGTTTGCAACAAGACGTAGCTTGTCTGATGCTTCCAATTCTGTAGCTAAACAGCTTGAGAGTCTGTTTACATCAATTTCGACCACTCAGAGGAAATTGTCTTCCCAACTTGATAATGTGGAATGTAGTTTGGATGAAAGTATAGAAAATACGGCTAGGACACAACAGGAGGTTGTTCAACTACAAGGAAGGACGGATACCATTAGCAGAGATTTTAAAAAAGTTCACCATGCAGTGCTGACTCTGGAAACAAAAATTAATAGAATTGAAGGGAAGCAGGGTGAAATTGCCGGTGGAGTATGGAAGTTATGTGACTATGCCCTGAACATGGAAAAGGGCATAAACGCTGAACGCATTCAGGCATCACCATCCAGTTTTTCCAGGCCAGTTCTTGAACTACCACCAGCCTCCCCCTCATCTTTGGTGACACCACCGAAGGCCTCGAGGCCAGCGCTTGAGCTACCCTCAGCATCACCCTCAAGGGGGAATAGTGGAATCTTGGAAGTGGTTGAGTCCTCAAATCGTGGCGAGGCTTCCAATGAGCTTCGTGCTCCAGAAGGCACAAACAATGCGTCTTCCAGTTCTGGTTGGTTCGGGTTTGGGTTTCCAACTTTGAATAGTTCGGCACTCCTGAGGACGCGAAGTGCTACAACCGGAATGGTTCAACGGAAACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

344

Amino Acids

37.14

Weight (kDa)

9.82

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 91 - 211 2.9e-41 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AbsI CCTCGAGG 1 cut(s) 796
AccI GTMKAC 1 cut(s) 232
AccII CGCG 1 cut(s) 994
AciI CCGC 2 cut(s) 152, 306
AfaI GTAC 1 cut(s) 271
AfeI AGCGCT 1 cut(s) 807
AfiI CCNNNNNNNGG 5 cut(s) 205, 444, 682, 836, 837
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 6 cut(s) 109, 533, 623, 749, 970, 1019
AjiI CACGTC 1 cut(s) 80
AjnI CCWGG 1 cut(s) 734
AluBI AGCT 8 cut(s) 25, 211, 343, 375, 401, 409, 814, 898
AluI AGCT 8 cut(s) 25, 211, 343, 375, 401, 409, 814, 898
Alw21I GWGCWC 1 cut(s) 907
Alw26I GTCTC 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 796
Aor51HI AGCGCT 1 cut(s) 807
AoxI GGCC 3 cut(s) 737, 792, 800
ApeKI GCWGC 1 cut(s) 5
ArsI GACNNNNNNTTYG 4 cut(s) 341, 373, 911, 943
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 708
AspLEI GCGC 2 cut(s) 44, 808
AsuHPI GGTGA 5 cut(s) 572, 647, 711, 790, 819
AvaI CYCGRG 1 cut(s) 796
AxyI CCTNAGG 1 cut(s) 986
BarI GAAGNNNNNNTAC 4 cut(s) 127, 159, 535, 567
BbsI GAAGAC 2 cut(s) 447, 921
Bbv12I GWGCWC 1 cut(s) 907
BbvCI CCTCAGC 1 cut(s) 820
BccI CCATC 2 cut(s) 175, 730
BceAI ACGGC 1 cut(s) 522
BciT130I CCWGG 1 cut(s) 736
BciVI GTATCC 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 100
BfaI CTAG 3 cut(s) 17, 273, 510
BfmI CTRYAG 1 cut(s) 396
BfoI RGCGCY 1 cut(s) 809
BfuI GTATCC 1 cut(s) 544
BisI GCNGC 2 cut(s) 6, 306
BlsI GCNGC 2 cut(s) 7, 307
Bme1390I CCNGG 1 cut(s) 736
BmeT110I CYCGRG 1 cut(s) 796
BmgBI CACGTC 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 736
BmsI GCATC 4 cut(s) 233, 373, 725, 833
BpiI GAAGAC 2 cut(s) 447, 921
BplI GAGNNNNNCTC 2 cut(s) 803, 835
BpmI CTGGAG 2 cut(s) 233, 891
Bpu10I CCTNAGC 1 cut(s) 820
BpuEI CTTGAG 3 cut(s) 431, 817, 830
BsaWI WCCGGW 1 cut(s) 1007
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 444, 682, 836, 837
Bse118I RCCGGY 1 cut(s) 644
Bse1I ACTGG 4 cut(s) 239, 726, 740, 933
Bse21I CCTNAGG 1 cut(s) 986
BseBI CCWGG 1 cut(s) 736
BseGI GGATG 2 cut(s) 493, 722
BseLI CCNNNNNNNGG 5 cut(s) 205, 444, 682, 836, 837
BseMII CTCAG 4 cut(s) 200, 455, 834, 977
BseNI ACTGG 4 cut(s) 239, 726, 740, 933
Bsh1236I CGCG 1 cut(s) 994
BshFI GGCC 3 cut(s) 739, 794, 802
BsiHKAI GWGCWC 1 cut(s) 907
BsiHKCI CYCGRG 1 cut(s) 796
BsiSI CCGG 3 cut(s) 149, 645, 1008
BslI CCNNNNNNNGG 5 cut(s) 205, 444, 682, 836, 837
BsmAI GTCTC 1 cut(s) 100
BsmI GAATGC 2 cut(s) 48, 708
BsnI GGCC 3 cut(s) 739, 794, 802
BsoBI CYCGRG 1 cut(s) 796
Bsp1286I GDGCHC 1 cut(s) 907
BspACI CCGC 2 cut(s) 152, 306
BspANI GGCC 3 cut(s) 739, 794, 802
BspCNI CTCAG 4 cut(s) 199, 454, 833, 978
BspFNI CGCG 1 cut(s) 994
BsrFI RCCGGY 1 cut(s) 644
BsrI ACTGG 4 cut(s) 239, 726, 740, 933
BssAI RCCGGY 1 cut(s) 644
Bst2UI CCWGG 1 cut(s) 736
BstC8I GCNNGC 3 cut(s) 23, 46, 804
BstDEI CTNAG 4 cut(s) 186, 441, 820, 986
BstENI CCTNNNNNAGG 1 cut(s) 203
BstF5I GGATG 2 cut(s) 493, 722
BstFNI CGCG 1 cut(s) 994
BstH2I RGCGCY 1 cut(s) 809
BstHHI GCGC 2 cut(s) 44, 808
BstMAI GTCTC 1 cut(s) 100
BstNI CCWGG 1 cut(s) 736
BstSCI CCNGG 1 cut(s) 734
BstSFI CTRYAG 1 cut(s) 396
BstUI CGCG 1 cut(s) 994
BstV2I GAAGAC 2 cut(s) 447, 921
Bsu36I CCTNAGG 1 cut(s) 986
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 3 cut(s) 739, 794, 802
BtrI CACGTC 1 cut(s) 80
BtsCI GGATG 2 cut(s) 493, 722
BtsI GCAGTG 1 cut(s) 594
BtsIMutI CAGTG 1 cut(s) 594
Cac8I GCNNGC 3 cut(s) 23, 46, 804
CfoI GCGC 2 cut(s) 44, 808
Cfr10I RCCGGY 1 cut(s) 644
CseI GACGC 2 cut(s) 915, 1000
Csp6I GTAC 1 cut(s) 270
CviAII CATG 3 cut(s) 352, 584, 682
CviQI GTAC 1 cut(s) 270
DdeI CTNAG 4 cut(s) 186, 441, 820, 986
DraI TTTAAA 1 cut(s) 571
Eco147I AGGCCT 1 cut(s) 794
Eco47III AGCGCT 1 cut(s) 807
Eco81I CCTNAGG 1 cut(s) 986
Eco88I CYCGRG 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 203
EcoRII CCWGG 1 cut(s) 734
FaeI CATG 3 cut(s) 355, 587, 685
FatI CATG 3 cut(s) 351, 583, 681
FblI GTMKAC 1 cut(s) 232
Fnu4HI GCNGC 2 cut(s) 6, 306
FokI GGATG 2 cut(s) 500, 709
Fsp4HI GCNGC 2 cut(s) 6, 306
FspBI CTAG 3 cut(s) 17, 273, 510
GlaI GCGC 2 cut(s) 43, 807
GluI GCNGC 2 cut(s) 6, 306
GsuI CTGGAG 2 cut(s) 233, 891
HaeII RGCGCY 1 cut(s) 809
HaeIII GGCC 3 cut(s) 739, 794, 802
HapII CCGG 3 cut(s) 149, 645, 1008
HgaI GACGC 2 cut(s) 915, 1000
HhaI GCGC 2 cut(s) 44, 808
Hin1II CATG 3 cut(s) 355, 587, 685
Hin6I GCGC 2 cut(s) 42, 806
HinP1I GCGC 2 cut(s) 42, 806
HincII GTYRAC 1 cut(s) 233
HindII GTYRAC 1 cut(s) 233
HindIII AAGCTT 2 cut(s) 23, 341
HinfI GANTC 6 cut(s) 173, 259, 415, 595, 849, 866
HpaII CCGG 3 cut(s) 149, 645, 1008
HphI GGTGA 5 cut(s) 572, 647, 711, 790, 819
Hpy166II GTNNAC 3 cut(s) 233, 423, 580
Hpy188I TCNGA 5 cut(s) 88, 180, 189, 382, 444
Hpy188III TCNNGA 6 cut(s) 109, 347, 599, 746, 908, 985
Hpy8I GTNNAC 3 cut(s) 233, 423, 580
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 7 cut(s) 68, 199, 325, 537, 617, 784, 905
HpyCH4IV ACGT 2 cut(s) 79, 370
HpyCH4V TGCA 3 cut(s) 68, 362, 587
HpyF3I CTNAG 4 cut(s) 186, 441, 820, 986
HpySE526I ACGT 2 cut(s) 79, 370
Hsp92II CATG 3 cut(s) 355, 587, 685
HspAI GCGC 2 cut(s) 42, 806
LmnI GCTCC 2 cut(s) 208, 910
LweI GCATC 4 cut(s) 233, 373, 725, 833
MaeI CTAG 3 cut(s) 17, 273, 510
MaeII ACGT 2 cut(s) 79, 370
MaeIII GTNAC 3 cut(s) 241, 665, 778
MboII GAAGA 2 cut(s) 447, 921
MhlI GDGCHC 1 cut(s) 907
MluCI AATT 9 cut(s) 126, 246, 288, 391, 429, 449, 609, 618, 639
MlyI GAGTC 5 cut(s) 167, 268, 424, 589, 875
MmeI TCCRAC 1 cut(s) 986
MroXI GAANNNNTTC 1 cut(s) 708
MseI TTAA 3 cut(s) 156, 570, 612
MspA1I CMGCKG 1 cut(s) 308
MspI CCGG 3 cut(s) 149, 645, 1008
MspR9I CCNGG 1 cut(s) 736
Mva1269I GAATGC 2 cut(s) 48, 708
MvaI CCWGG 1 cut(s) 736
MvnI CGCG 1 cut(s) 994
NlaIII CATG 3 cut(s) 355, 587, 685
NmuCI GTSAC 2 cut(s) 665, 778
PaeR7I CTCGAG 1 cut(s) 796
PceI AGGCCT 1 cut(s) 794
PctI GAATGC 2 cut(s) 48, 708
PdmI GAANNNNTTC 1 cut(s) 708
PfeI GAWTC 1 cut(s) 849
PkrI GCNGC 2 cut(s) 7, 307
PleI GAGTC 5 cut(s) 167, 267, 423, 589, 874
PpsI GAGTC 5 cut(s) 167, 267, 423, 589, 874
PshBI ATTAAT 1 cut(s) 612
PsiI TTATAA 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 734
PspGI CCWGG 1 cut(s) 734
PspXI VCTCGAGB 1 cut(s) 796
RsaI GTAC 1 cut(s) 271
RsaNI GTAC 1 cut(s) 270
SalI GTCGAC 1 cut(s) 231
SaqAI TTAA 3 cut(s) 156, 570, 612
SatI GCNGC 2 cut(s) 6, 306
SchI GAGTC 5 cut(s) 167, 268, 424, 589, 875
ScrFI CCNGG 1 cut(s) 736
SduI GDGCHC 1 cut(s) 907
SfaNI GCATC 4 cut(s) 233, 373, 725, 833
SfcI CTRYAG 1 cut(s) 396
Sfr274I CTCGAG 1 cut(s) 796
SlaI CTCGAG 1 cut(s) 796
SmlI CTYRAG 4 cut(s) 410, 796, 809, 832
SmoI CTYRAG 4 cut(s) 410, 796, 809, 832
Sse9I AATT 9 cut(s) 126, 246, 288, 391, 429, 449, 609, 618, 639
SseBI AGGCCT 1 cut(s) 794
SsiI CCGC 2 cut(s) 152, 306
SspMI CTAG 3 cut(s) 17, 273, 510
StuI AGGCCT 1 cut(s) 794
StyD4I CCNGG 1 cut(s) 734
TaiI ACGT 2 cut(s) 82, 373
TaqI TCGA 4 cut(s) 232, 262, 434, 797
TasI AATT 9 cut(s) 126, 246, 288, 391, 429, 449, 609, 618, 639
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 1 cut(s) 849
Tru1I TTAA 3 cut(s) 156, 570, 612
Tru9I TTAA 3 cut(s) 156, 570, 612
TscAI CASTG 1 cut(s) 594
TseFI GTSAC 2 cut(s) 665, 778
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 665, 778
TspDTI ATGAA 2 cut(s) 206, 504
TspGWI ACGGA 2 cut(s) 217, 563
TspRI CASTG 1 cut(s) 594
VspI ATTAAT 1 cut(s) 612
XagI CCTNNNNNAGG 1 cut(s) 203
XhoI CTCGAG 1 cut(s) 796
XmiI GTMKAC 1 cut(s) 232
XmnI GAANNNNTTC 1 cut(s) 708
XspI CTAG 3 cut(s) 17, 273, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.