AT1G43780

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005327
Physical Location & Seq
Forward (+)
16563811 .. 16567399
3589 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G43780.1

Sequence Viewer

Length: 1440 bp
ATGGTGGGAGGTAAGTGGCGGTTCCTAGAAGTAGCAGTCGTAGTAATGGTGTTGCAGTGGTCGTGCGATTATAATGGTAATTTAGCTGAAGGATTTCCAGTACAAGATCTTGTGACAAAGCTGCCTGGACAACCTGAAGTGGCATTCAGACAATTTGCGGGCTATGTCGATATCGATGTCAAAGCTGGTCGGAGTCTCTTTTATTACTTCGTCGAAGCTGAGAAGCAACCCCACTCTAAACCGTTAACTCTTTGGCTCAATGGAGGACCAGGCTGTTCTTCCATAGGAGGAGGAGCTTTCACAGAGTTGGGCCCATTTTACCCCACCGGAGATGCTCGTGGCCTCCGCAGAAATCCCAAATCTTGGAACAAAGCCTCAAATCTATTGTTTGTGGATTCACCTGCTGGAGTAGGATGGTCTTATTCTAACACAACTTCTGATTACACCACTGGCGATGAATCCACCGCCAAGGATATGTTGGTGTTCATGTTAAGATGGTTAGAAAAATTTCCACAATTCAAGACTCGAAACCTATTTCTCGCGGGCGAAAGCTACGCAGGACACTATGTACCTCAACTAGCCGATGTGATACTCGAATACAACGCACAACGGTCAAATAGATTCAAGTTCAATCTCAAAGGGATTGCAATTGGAAATCCGCTTTTGAAGCTTGACCGCGATGTTCCAGCGATATATGAGTTCTTCTGGTCACACGGGATGATCTCTGATGAACTTGGCCTCACTATCATGAACCAATGCGACTTTGAAGATTACACATTCACAGATTCACACAACATAAGCAAACTTTGCGAAGCTGCAGTGAATCAAGCTGGAACCATCATCACTCAATATGTTAATTACTACGACATTCTCCTCGATGTATGCTACCCGTCTCTCTTCGAGCAAGAGCTTAGGCTCAAGAAAATGGGTACAAGGATGAGTTTTGGGGTGGACGTGTGCATGAGCTTTGAAGAGCAATTGTATTTAAATCTTCCTGAGGTTCAAAAGGCTCTCCATGCTAACCGAACTAAACTACCGTATGAATGGTCTATGTGCAGCAGCCTCTTGAACTACAAATACACTGACGGGAACGCCAACATGCTTCCAATTCTTAAAAGAATCGTGAAGAGCAAAGTCCCTGTTTGGGTCTTCAGTGGAGATGAAGATTCTGTAATTCCACTTCTTGGTTCAAGAACTTTGGTAAAAGAACTTGCAGATGATCTCAACTTCAATACCACAGTTCCTTATGGAGCTTGGTTCGACAAAGGACAGGTTGGCGGATGGGTAGTAGAGTATGGGAATCTACTGACATTCGCAACGGTGAGAGGAGCGGCTCATATGGTGCCGTACTCGCAGCCTTCACGAGCTTTACATTTGTTCACCAGCTTTGTGCTTGGCCGGAAATTGCCACACAAGTCGCCTCCTGCTCTTCATGATTGA

Protein Analysis

479

Amino Acids

53.92

Weight (kDa)

6.18

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 42 - 465 1.7e-140 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 72
AarI CACCTGC 1 cut(s) 409
Acc36I ACCTGC 1 cut(s) 409
AccB1I GGYRCC 1 cut(s) 1342
AccB7I CCANNNNNTGG 2 cut(s) 363, 1184
AccBSI CCGCTC 1 cut(s) 1331
AccII CGCG 2 cut(s) 542, 678
AciI CCGC 9 cut(s) 19, 158, 346, 465, 542, 659, 676, 1278, 1331
AcoI YGGCCR 1 cut(s) 1396
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 3 cut(s) 108, 156, 1135
AfaI GTAC 4 cut(s) 102, 570, 931, 1349
AfiI CCNNNNNNNGG 3 cut(s) 363, 1144, 1184
AflIII ACRYGT 1 cut(s) 954
AjiI CACGTC 1 cut(s) 955
AjnI CCWGG 2 cut(s) 124, 268
Alw26I GTCTC 2 cut(s) 200, 897
AoxI GGCC 4 cut(s) 310, 340, 736, 1396
ApaI GGGCCC 1 cut(s) 314
ApeKI GCWGC 5 cut(s) 121, 815, 1056, 1059, 1354
ApoI RAATTY 1 cut(s) 506
Asp700I GAANNNNTTC 2 cut(s) 93, 507
AspS9I GGNCC 3 cut(s) 266, 310, 311
AsuHPI GGTGA 3 cut(s) 390, 1333, 1372
AvaII GGWCC 1 cut(s) 266
AxyI CCTNAGG 1 cut(s) 996
BaeGI GKGCMC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 1342
BanII GRGCYC 1 cut(s) 314
BarI GAAGNNNNNNTAC 2 cut(s) 1164, 1196
BauI CACGAG 2 cut(s) 336, 1362
BbsI GAAGAC 1 cut(s) 1141
BbvI GCAGC 5 cut(s) 108, 802, 1068, 1071, 1366
BccI CCATC 4 cut(s) 408, 489, 845, 1275
BceAI ACGGC 1 cut(s) 1330
BciT130I CCWGG 2 cut(s) 126, 270
BcoDI GTCTC 2 cut(s) 200, 897
BfaI CTAG 2 cut(s) 26, 578
BfmI CTRYAG 1 cut(s) 816
BfuAI ACCTGC 1 cut(s) 409
BglII AGATCT 1 cut(s) 106
BisI GCNGC 6 cut(s) 122, 816, 1057, 1060, 1332, 1355
BlsI GCNGC 6 cut(s) 123, 817, 1058, 1061, 1333, 1356
Bme1390I CCNGG 2 cut(s) 126, 270
Bme18I GGWCC 1 cut(s) 266
BmgBI CACGTC 1 cut(s) 955
BmgT120I GGNCC 3 cut(s) 266, 310, 311
BmiI GGNNCC 4 cut(s) 23, 312, 835, 1344
BmrFI CCNGG 2 cut(s) 126, 270
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 1141
BpmI CTGGAG 1 cut(s) 426
Bpu10I CCTNAGC 1 cut(s) 911
BpuEI CTTGAG 1 cut(s) 902
Bsa29I ATCGAT 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 468
BsaWI WCCGGW 1 cut(s) 326
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 1144, 1184
Bse1I ACTGG 2 cut(s) 98, 454
Bse21I CCTNAGG 1 cut(s) 996
BseBI CCWGG 2 cut(s) 126, 270
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 468
BseGI GGATG 4 cut(s) 419, 723, 942, 1286
BseLI CCNNNNNNNGG 3 cut(s) 363, 1144, 1184
BseMII CTCAG 2 cut(s) 210, 987
BseNI ACTGG 2 cut(s) 98, 454
BseRI GAGGAG 4 cut(s) 303, 306, 863, 1341
BseSI GKGCMC 1 cut(s) 314
BseXI GCAGC 5 cut(s) 108, 802, 1068, 1071, 1366
BsgI GTGCAG 1 cut(s) 1075
Bsh1236I CGCG 2 cut(s) 542, 678
BshFI GGCC 4 cut(s) 312, 342, 738, 1398
BshNI GGYRCC 1 cut(s) 1342
BshVI ATCGAT 1 cut(s) 174
BsiSI CCGG 2 cut(s) 327, 1399
BslFI GGGAC 1 cut(s) 1121
BslI CCNNNNNNNGG 3 cut(s) 363, 1144, 1184
BsmAI GTCTC 2 cut(s) 200, 897
BsmBI CGTCTC 1 cut(s) 897
BsmFI GGGAC 1 cut(s) 1121
BsmI GAATGC 1 cut(s) 143
BsnI GGCC 4 cut(s) 312, 342, 738, 1398
Bsp120I GGGCCC 1 cut(s) 310
Bsp1286I GDGCHC 1 cut(s) 314
Bsp143I GATC 3 cut(s) 106, 720, 1219
BspACI CCGC 9 cut(s) 19, 158, 346, 465, 542, 659, 676, 1278, 1331
BspANI GGCC 4 cut(s) 312, 342, 738, 1398
BspCNI CTCAG 2 cut(s) 211, 988
BspDI ATCGAT 1 cut(s) 174
BspFNI CGCG 2 cut(s) 542, 678
BspHI TCATGA 2 cut(s) 747, 1432
BspLI GGNNCC 4 cut(s) 23, 312, 835, 1344
BspMAI CTGCAG 1 cut(s) 820
BspMI ACCTGC 1 cut(s) 409
BspQI GCTCTTC 3 cut(s) 966, 1121, 1434
BspT107I GGYRCC 1 cut(s) 1342
BsrBI CCGCTC 1 cut(s) 1331
BsrI ACTGG 2 cut(s) 98, 454
BssECI CCNNGG 1 cut(s) 468
BssMI GATC 3 cut(s) 106, 720, 1219
BssSI CACGAG 2 cut(s) 336, 1362
BssT1I CCWWGG 1 cut(s) 468
Bst2BI CACGAG 2 cut(s) 336, 1362
Bst2UI CCWGG 2 cut(s) 126, 270
Bst4CI ACNGT 5 cut(s) 243, 612, 1038, 1240, 1321
Bst6I CTCTTC 4 cut(s) 902, 966, 1121, 1434
BstAPI GCANNNNNTGC 1 cut(s) 807
BstC8I GCNNGC 2 cut(s) 160, 544
BstDEI CTNAG 3 cut(s) 219, 911, 996
BstF5I GGATG 4 cut(s) 419, 723, 942, 1286
BstFNI CGCG 2 cut(s) 542, 678
BstKTI GATC 3 cut(s) 109, 723, 1222
BstMAI GTCTC 2 cut(s) 200, 897
BstMBI GATC 3 cut(s) 106, 720, 1219
BstMWI GCNNNNNNNGC 4 cut(s) 667, 807, 1016, 1351
BstNI CCWGG 2 cut(s) 126, 270
BstNSI RCATGY 1 cut(s) 1102
BstSCI CCNGG 2 cut(s) 124, 268
BstSFI CTRYAG 1 cut(s) 816
BstSLI GKGCMC 1 cut(s) 314
BstUI CGCG 2 cut(s) 542, 678
BstV1I GCAGC 5 cut(s) 108, 802, 1068, 1071, 1366
BstV2I GAAGAC 1 cut(s) 1141
BstX2I RGATCY 1 cut(s) 106
BstYI RGATCY 1 cut(s) 106
Bsu15I ATCGAT 1 cut(s) 174
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 4 cut(s) 312, 342, 738, 1398
BsuTUI ATCGAT 1 cut(s) 174
BtgZI GCGATG 2 cut(s) 468, 693
BtrI CACGTC 1 cut(s) 955
BtsCI GGATG 4 cut(s) 419, 723, 942, 1286
BtsI GCAGTG 2 cut(s) 62, 825
BtsIMutI CAGTG 5 cut(s) 62, 447, 825, 1080, 1159
BveI ACCTGC 1 cut(s) 409
Cac8I GCNNGC 2 cut(s) 160, 544
CciI TCATGA 2 cut(s) 747, 1432
Cfr13I GGNCC 3 cut(s) 266, 310, 311
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 4 cut(s) 101, 569, 930, 1348
CviAII CATG 6 cut(s) 487, 748, 961, 1016, 1099, 1433
CviQI GTAC 4 cut(s) 101, 569, 930, 1348
DdeI CTNAG 3 cut(s) 219, 911, 996
DpnI GATC 3 cut(s) 108, 722, 1221
DpnII GATC 3 cut(s) 106, 720, 1219
DraI TTTAAA 1 cut(s) 987
EaeI YGGCCR 1 cut(s) 1396
Eam1104I CTCTTC 4 cut(s) 902, 966, 1121, 1434
EarI CTCTTC 4 cut(s) 902, 966, 1121, 1434
EciI GGCGGA 1 cut(s) 1293
Eco130I CCWWGG 1 cut(s) 468
Eco24I GRGCYC 1 cut(s) 314
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 1 cut(s) 266
Eco57I CTGAAG 3 cut(s) 108, 156, 1135
Eco81I CCTNAGG 1 cut(s) 996
EcoRII CCWGG 2 cut(s) 124, 268
EcoRV GATATC 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 468
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 468
Esp3I CGTCTC 1 cut(s) 897
FaeI CATG 6 cut(s) 490, 751, 964, 1019, 1102, 1436
FaqI GGGAC 1 cut(s) 1121
FatI CATG 6 cut(s) 486, 747, 960, 1015, 1098, 1432
FauI CCCGC 2 cut(s) 151, 535
FauNDI CATATG 1 cut(s) 1338
Fnu4HI GCNGC 6 cut(s) 122, 816, 1057, 1060, 1332, 1355
FokI GGATG 4 cut(s) 426, 730, 949, 1293
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 6 cut(s) 122, 816, 1057, 1060, 1332, 1355
FspBI CTAG 2 cut(s) 26, 578
GluI GCNGC 6 cut(s) 122, 816, 1057, 1060, 1332, 1355
GsuI CTGGAG 1 cut(s) 426
HaeIII GGCC 4 cut(s) 312, 342, 738, 1398
HapII CCGG 2 cut(s) 327, 1399
Hin1II CATG 6 cut(s) 490, 751, 964, 1019, 1102, 1436
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 668
HpaI GTTAAC 1 cut(s) 246
HpaII CCGG 2 cut(s) 327, 1399
HphI GGTGA 3 cut(s) 390, 1333, 1372
Hpy166II GTNNAC 3 cut(s) 246, 952, 1380
Hpy188I TCNGA 4 cut(s) 149, 192, 439, 727
Hpy188III TCNNGA 9 cut(s) 520, 748, 919, 995, 1066, 1123, 1191, 1362, 1433
Hpy8I GTNNAC 3 cut(s) 246, 952, 1380
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 83, 1368
HpyCH4III ACNGT 5 cut(s) 243, 612, 1038, 1240, 1321
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 6 cut(s) 55, 647, 818, 960, 1056, 1214
HpyF10VI GCNNNNNNNGC 4 cut(s) 667, 807, 1016, 1351
HpyF3I CTNAG 3 cut(s) 219, 911, 996
HpySE526I ACGT 1 cut(s) 954
Hsp92II CATG 6 cut(s) 490, 751, 964, 1019, 1102, 1436
KspAI GTTAAC 1 cut(s) 246
Kzo9I GATC 3 cut(s) 106, 720, 1219
LguI GCTCTTC 3 cut(s) 966, 1121, 1434
LmnI GCTCC 3 cut(s) 293, 1250, 1328
Lsp1109I GCAGC 5 cut(s) 108, 802, 1068, 1071, 1366
LweI GCATC 1 cut(s) 322
MaeI CTAG 2 cut(s) 26, 578
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 2 cut(s) 112, 708
MalI GATC 3 cut(s) 108, 722, 1221
MbiI CCGCTC 1 cut(s) 1331
MboI GATC 3 cut(s) 106, 720, 1219
MfeI CAATTG 2 cut(s) 648, 977
MflI RGATCY 1 cut(s) 106
MhlI GDGCHC 1 cut(s) 314
MlyI GAGTC 2 cut(s) 202, 517
MmeI TCCRAC 1 cut(s) 170
MroXI GAANNNNTTC 2 cut(s) 93, 507
MseI TTAA 5 cut(s) 245, 491, 855, 986, 1113
MslI CAYNNNNRTG 1 cut(s) 746
MspI CCGG 2 cut(s) 327, 1399
MspR9I CCNGG 2 cut(s) 126, 270
MunI CAATTG 2 cut(s) 648, 977
Mva1269I GAATGC 1 cut(s) 143
MvaI CCWGG 2 cut(s) 126, 270
MvnI CGCG 2 cut(s) 542, 678
MwoI GCNNNNNNNGC 4 cut(s) 667, 807, 1016, 1351
NdeI CATATG 1 cut(s) 1338
NdeII GATC 3 cut(s) 106, 720, 1219
NlaIII CATG 6 cut(s) 490, 751, 964, 1019, 1102, 1436
NlaIV GGNNCC 4 cut(s) 23, 312, 835, 1344
NmuCI GTSAC 2 cut(s) 112, 708
NspI RCATGY 1 cut(s) 1102
PagI TCATGA 2 cut(s) 747, 1432
PaqCI CACCTGC 1 cut(s) 409
PciSI GCTCTTC 3 cut(s) 966, 1121, 1434
PctI GAATGC 1 cut(s) 143
PdmI GAANNNNTTC 2 cut(s) 93, 507
PfeI GAWTC 8 cut(s) 395, 458, 621, 785, 823, 1119, 1166, 1300
PflMI CCANNNNNTGG 2 cut(s) 363, 1184
PkrI GCNGC 6 cut(s) 123, 817, 1058, 1061, 1333, 1356
PleI GAGTC 2 cut(s) 201, 517
PpsI GAGTC 2 cut(s) 201, 517
PsiI TTATAA 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 124, 268
PspGI CCWGG 2 cut(s) 124, 268
PspN4I GGNNCC 4 cut(s) 23, 312, 835, 1344
PspOMI GGGCCC 1 cut(s) 310
PspPI GGNCC 3 cut(s) 266, 310, 311
PsrI GAACNNNNNNTAC 2 cut(s) 1061, 1093
PstI CTGCAG 1 cut(s) 820
PsuI RGATCY 1 cut(s) 106
RsaI GTAC 4 cut(s) 102, 570, 931, 1349
RsaNI GTAC 4 cut(s) 101, 569, 930, 1348
RseI CAYNNNNRTG 1 cut(s) 746
SapI GCTCTTC 3 cut(s) 966, 1121, 1434
SaqAI TTAA 5 cut(s) 245, 491, 855, 986, 1113
SatI GCNGC 6 cut(s) 122, 816, 1057, 1060, 1332, 1355
Sau3AI GATC 3 cut(s) 106, 720, 1219
Sau96I GGNCC 3 cut(s) 266, 310, 311
SchI GAGTC 2 cut(s) 202, 517
ScrFI CCNGG 2 cut(s) 126, 270
SduI GDGCHC 1 cut(s) 314
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 1 cut(s) 816
SinI GGWCC 1 cut(s) 266
SmiI ATTTAAAT 1 cut(s) 987
SmiMI CAYNNNNRTG 1 cut(s) 746
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SsiI CCGC 9 cut(s) 19, 158, 346, 465, 542, 659, 676, 1278, 1331
SspMI CTAG 2 cut(s) 26, 578
StyD4I CCNGG 2 cut(s) 124, 268
StyI CCWWGG 1 cut(s) 468
SwaI ATTTAAAT 1 cut(s) 987
TaaI ACNGT 5 cut(s) 243, 612, 1038, 1240, 1321
TaiI ACGT 1 cut(s) 957
TaqI TCGA 8 cut(s) 168, 174, 213, 526, 594, 876, 900, 1260
TatI WGTACW 1 cut(s) 100
TauI GCSGC 1 cut(s) 1334
TfiI GAWTC 8 cut(s) 395, 458, 621, 785, 823, 1119, 1166, 1300
Tru1I TTAA 5 cut(s) 245, 491, 855, 986, 1113
Tru9I TTAA 5 cut(s) 245, 491, 855, 986, 1113
TscAI CASTG 5 cut(s) 62, 454, 825, 1087, 1159
TseFI GTSAC 2 cut(s) 112, 708
TseI GCWGC 5 cut(s) 121, 815, 1056, 1059, 1354
Tsp45I GTSAC 2 cut(s) 112, 708
TspDTI ATGAA 7 cut(s) 471, 475, 744, 764, 1056, 1176, 1421
TspRI CASTG 5 cut(s) 62, 454, 825, 1087, 1159
Van91I CCANNNNNTGG 2 cut(s) 363, 1184
VpaK11BI GGWCC 1 cut(s) 266
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 1102
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmnI GAANNNNTTC 2 cut(s) 93, 507
XspI CTAG 2 cut(s) 26, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.