AT5G42230

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005327
Physical Location & Seq
Forward (+)
16880592 .. 16884462
3871 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G42230.2

Sequence Viewer

Length: 1410 bp
ATGGCTATTGTGTCGTTGCGCGATGTGGCCATGGTGATGGTGACAGTGCAGGTGTTTGCACGAGGGTATCCGGAAACAGATTTGGTTGTGAGGCTTCCTGGTCAACCAAAGGTTGTATTCAGACAGTACGCAGGGTATGTTGATCTCGACTTGAATGCTGGCCGTAGCCTTTTCTACTACTTCGTGGAAGCTGAAAAACACCCTGACACAAAACCGTTAACCTTGTGGCTCAATGGAGGTCCAGGTTGTTCTTCTGTTGGTGGAGGAGCTTTCACTGAGCTAGGTCCATTTTACCCCACAGGATATGGGCGTGGTCTTCGCATTAACTCCATGTCGTGGAACAAAGCCTCAAACCTGCTATTTGTGGACTCACCGGCTGGAGTTGGTTGGTCTTACTCCAACCGAAGCTCTGATTACAACGCTGGGGACAAGTCTGCGGCCAGCGATATGCTCGTGTTCTTGTTGAGATGGTTCGATAAGTTCCCGGAGTTGAAGTCTCACGATCTCTTTCTCACTGGCGAAAGCTATGCAGGACATTACATACCTCAACTGGCTGATGCGATTCTCAGCTATAACTCACGCTCAAGTGGGTTCAAATTCAACATCAAAGGCATTGCAATAGGGAATCCACTTCTGAAGCTTGACAGGGACATTCCAGCTGTATACGAGTTCTTCTGGTCGCATGGCATGATCTCTGAGGTAGTGGGACGTACAATCAAGATTCAATGTGACTTCTCACATTACACATATGCATACCCCCATAACGTAAGTGATGCCTGCAACGATGCTATCCGTGAAGCCGGAGACATCACCACTGAGTATGTCAATACCTTTGATGTTCTCCCTGACCTATGCTACCCATCTATTGCCCTGCAAGAGCTAAGGCTTAAACAAATGGCTACTAAGATGAGCATGGGAGTGGATGTGTGTATGAACTACGAAAGACAGTTCTACCTAAATATCCCAGAGGTACAGATGGCTCTTCACGCAAACCGCACAAACCTGCCTTATTCATGGTCTTTGTGCAGCAACCTGTTAAACTACTCTGCCATCGATGTAAACACCAACATGCTTCCGACTCTGAAGAGAATTATACAGAACAAAATTCCTGTTAGGATCTTCAGTGGAGATCAAGATTCTGTAGTTCCATTTCTGGGAACACGAACTATCGTAGGAGAACTCGCCAACGATCTCAATTTCAAGACGACAGTTCCATATGGAGTGTGGTTCCACAAAAGACAGGTTGGAGGCTGGGCCATCGAGTATGGAAACCTACTAACTTTTGCAACAGTGAGAGGAGCGGCTCATGCGGTGGCGTACACGCAGCCCTCACGAGCTCTGCATCTATTCAGCACCTTTCTTCGTGGGCAGAGATTGCCCAACAAGACAGATATTGCTATGCATGACTGA

Protein Analysis

469

Amino Acids

52.54

Weight (kDa)

7.16

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 33 - 456 1.7e-129 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 40
Acc36I ACCTGC 3 cut(s) 40, 363, 1011
AccB7I CCANNNNNTGG 1 cut(s) 336
AccBSI CCGCTC 1 cut(s) 1301
AccI GTMKAC 1 cut(s) 663
AccII CGCG 1 cut(s) 21
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 4 cut(s) 437, 994, 1301, 1310
AclWI GGATC 1 cut(s) 1124
AcoI YGGCCR 3 cut(s) 27, 160, 438
AcsI RAATTY 2 cut(s) 596, 1104
AcuI CTGAAG 3 cut(s) 656, 1103, 1105
AfaI GTAC 4 cut(s) 128, 712, 972, 1319
AfiI CCNNNNNNNGG 2 cut(s) 336, 1154
AgsI TTSAA 6 cut(s) 154, 493, 595, 601, 725, 1201
AjnI CCWGG 2 cut(s) 97, 241
Alw21I GWGCWC 1 cut(s) 1339
Alw26I GTCTC 2 cut(s) 501, 798
AlwI GGATC 1 cut(s) 1124
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 4 cut(s) 27, 160, 438, 1254
ApeKI GCWGC 2 cut(s) 1026, 1324
ApoI RAATTY 2 cut(s) 596, 1104
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 3 cut(s) 239, 284, 1254
AsuC2I CCSGG 1 cut(s) 485
AsuHPI GGTGA 4 cut(s) 46, 52, 363, 802
AvaII GGWCC 2 cut(s) 239, 284
BaeI ACNNNNGTAYC 2 cut(s) 50, 83
BalI TGGCCA 1 cut(s) 29
BanII GRGCYC 1 cut(s) 1339
BauI CACGAG 3 cut(s) 60, 452, 1332
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 1339
BbvI GCAGC 2 cut(s) 1038, 1336
BccI CCATC 6 cut(s) 31, 462, 868, 970, 1058, 1265
BceAI ACGGC 1 cut(s) 147
BcgI CGANNNNNNTGC 4 cut(s) 137, 171, 509, 543
BciT130I CCWGG 2 cut(s) 99, 243
BciVI GTATCC 1 cut(s) 78
BcnI CCSGG 1 cut(s) 485
BcoDI GTCTC 2 cut(s) 501, 798
BfaI CTAG 1 cut(s) 281
BfmI CTRYAG 1 cut(s) 1140
BfuAI ACCTGC 3 cut(s) 40, 363, 1011
BfuI GTATCC 1 cut(s) 78
BisI GCNGC 4 cut(s) 438, 1027, 1302, 1325
BlsI GCNGC 4 cut(s) 439, 1028, 1303, 1326
Bme1390I CCNGG 3 cut(s) 99, 243, 485
Bme18I GGWCC 2 cut(s) 239, 284
BmgT120I GGNCC 3 cut(s) 239, 284, 1254
BmiI GGNNCC 1 cut(s) 1229
BmrFI CCNGG 3 cut(s) 99, 243, 485
BmsI GCATC 4 cut(s) 547, 763, 775, 1351
BpiI GAAGAC 1 cut(s) 308
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 568
BpuMI CCSGG 1 cut(s) 485
Bsa29I ATCGAT 1 cut(s) 1053
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 909, 939
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 1154
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 2 cut(s) 520, 555
Bse3DI GCAATG 1 cut(s) 612
BseAI TCCGGA 1 cut(s) 70
BseBI CCWGG 2 cut(s) 99, 243
BseCI ATCGAT 1 cut(s) 1053
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 928
BseLI CCNNNNNNNGG 2 cut(s) 336, 1154
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 4 cut(s) 267, 580, 687, 807
BseNI ACTGG 2 cut(s) 520, 555
BseRI GAGGAG 2 cut(s) 279, 1311
BseXI GCAGC 2 cut(s) 1038, 1336
BseYI CCCAGC 2 cut(s) 422, 1251
BsgI GTGCAG 2 cut(s) 68, 1045
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 4 cut(s) 29, 162, 440, 1256
BshVI ATCGAT 1 cut(s) 1053
BsiHKAI GWGCWC 1 cut(s) 1339
BsiSI CCGG 4 cut(s) 71, 374, 485, 801
BslFI GGGAC 3 cut(s) 440, 662, 720
BslI CCNNNNNNNGG 2 cut(s) 336, 1154
BsmAI GTCTC 2 cut(s) 501, 798
BsmFI GGGAC 3 cut(s) 440, 662, 720
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 4 cut(s) 29, 162, 440, 1256
Bsp1286I GDGCHC 1 cut(s) 1339
Bsp13I TCCGGA 1 cut(s) 70
Bsp143I GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 4 cut(s) 437, 994, 1301, 1310
BspANI GGCC 4 cut(s) 29, 162, 440, 1256
BspCNI CTCAG 4 cut(s) 268, 579, 688, 808
BspDI ATCGAT 1 cut(s) 1053
BspEI TCCGGA 1 cut(s) 70
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 1229
BspMI ACCTGC 3 cut(s) 40, 363, 1011
BspPI GGATC 1 cut(s) 1124
BspQI GCTCTTC 1 cut(s) 987
BsrBI CCGCTC 1 cut(s) 1301
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 373
BsrI ACTGG 2 cut(s) 520, 555
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
BssNAI GTATAC 1 cut(s) 664
BssSI CACGAG 3 cut(s) 60, 452, 1332
BssT1I CCWWGG 1 cut(s) 30
Bst1107I GTATAC 1 cut(s) 664
Bst2BI CACGAG 3 cut(s) 60, 452, 1332
Bst2UI CCWGG 2 cut(s) 99, 243
Bst4CI ACNGT 6 cut(s) 46, 126, 216, 948, 1210, 1291
Bst6I CTCTTC 2 cut(s) 987, 1079
BstAPI GCANNNNNTGC 1 cut(s) 1375
BstC8I GCNNGC 3 cut(s) 160, 442, 778
BstDEI CTNAG 6 cut(s) 276, 566, 696, 816, 881, 903
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 928
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 6 cut(s) 145, 505, 693, 1119, 1132, 1192
BstMAI GTCTC 2 cut(s) 501, 798
BstMBI GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
BstMWI GCNNNNNNNGC 3 cut(s) 986, 1307, 1375
BstNI CCWGG 2 cut(s) 99, 243
BstNSI RCATGY 1 cut(s) 1072
BstSCI CCNGG 3 cut(s) 97, 241, 483
BstSFI CTRYAG 1 cut(s) 1140
BstUI CGCG 1 cut(s) 21
BstV1I GCAGC 2 cut(s) 1038, 1336
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 1116
BstXI CCANNNNNNTGG 1 cut(s) 37
BstYI RGATCY 1 cut(s) 1116
BstZ17I GTATAC 1 cut(s) 664
Bsu15I ATCGAT 1 cut(s) 1053
BsuI GTATCC 1 cut(s) 78
BsuRI GGCC 4 cut(s) 29, 162, 440, 1256
BsuTUI ATCGAT 1 cut(s) 1053
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 1 cut(s) 928
BtsIMutI CAGTG 6 cut(s) 51, 273, 513, 813, 1129, 1296
BveI ACCTGC 3 cut(s) 40, 363, 1011
Cac8I GCNNGC 3 cut(s) 160, 442, 778
CfoI GCGC 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 3 cut(s) 239, 284, 1254
ClaI ATCGAT 1 cut(s) 1053
Csp6I GTAC 4 cut(s) 127, 711, 971, 1318
CviAII CATG 9 cut(s) 31, 331, 683, 688, 913, 1014, 1069, 1307, 1403
CviQI GTAC 4 cut(s) 127, 711, 971, 1318
DdeI CTNAG 6 cut(s) 276, 566, 696, 816, 881, 903
DpnI GATC 6 cut(s) 144, 504, 692, 1118, 1131, 1191
DpnII GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
EaeI YGGCCR 3 cut(s) 27, 160, 438
Eam1104I CTCTTC 2 cut(s) 987, 1079
EarI CTCTTC 2 cut(s) 987, 1079
Ecl136II GAGCTC 1 cut(s) 1337
Eco130I CCWWGG 1 cut(s) 30
Eco24I GRGCYC 1 cut(s) 1339
Eco47I GGWCC 2 cut(s) 239, 284
Eco53kI GAGCTC 1 cut(s) 1337
Eco57I CTGAAG 3 cut(s) 656, 1103, 1105
EcoICRI GAGCTC 1 cut(s) 1337
EcoRII CCWGG 2 cut(s) 97, 241
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 2 cut(s) 754, 1404
EcoT38I GRGCYC 1 cut(s) 1339
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 9 cut(s) 34, 334, 686, 691, 916, 1017, 1072, 1310, 1406
FaqI GGGAC 3 cut(s) 440, 662, 720
FatI CATG 9 cut(s) 30, 330, 682, 687, 912, 1013, 1068, 1306, 1402
FauNDI CATATG 2 cut(s) 748, 1216
FblI GTMKAC 1 cut(s) 663
Fnu4HI GCNGC 4 cut(s) 438, 1027, 1302, 1325
FokI GGATG 1 cut(s) 935
FriOI GRGCYC 1 cut(s) 1339
Fsp4HI GCNGC 4 cut(s) 438, 1027, 1302, 1325
FspBI CTAG 1 cut(s) 281
GlaI GCGC 1 cut(s) 20
GluI GCNGC 4 cut(s) 438, 1027, 1302, 1325
GsaI CCCAGC 2 cut(s) 426, 1255
GsuI CTGGAG 1 cut(s) 399
HaeIII GGCC 4 cut(s) 29, 162, 440, 1256
HapII CCGG 4 cut(s) 71, 374, 485, 801
HhaI GCGC 1 cut(s) 21
Hin1II CATG 9 cut(s) 34, 334, 686, 691, 916, 1017, 1072, 1310, 1406
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 2 cut(s) 104, 219
HindII GTYRAC 2 cut(s) 104, 219
HindIII AAGCTT 1 cut(s) 638
HinfI GANTC 6 cut(s) 368, 562, 625, 721, 1078, 1136
HpaI GTTAAC 1 cut(s) 219
HpaII CCGG 4 cut(s) 71, 374, 485, 801
HphI GGTGA 4 cut(s) 46, 52, 363, 802
Hpy166II GTNNAC 6 cut(s) 104, 219, 367, 664, 1060, 1320
Hpy188I TCNGA 6 cut(s) 122, 412, 636, 697, 1077, 1083
Hpy188III TCNNGA 7 cut(s) 71, 146, 500, 718, 1133, 1201, 1332
Hpy8I GTNNAC 6 cut(s) 104, 219, 367, 664, 1060, 1320
HpyCH4III ACNGT 6 cut(s) 46, 126, 216, 948, 1210, 1291
HpyCH4IV ACGT 2 cut(s) 709, 765
HpyF10VI GCNNNNNNNGC 3 cut(s) 986, 1307, 1375
HpyF3I CTNAG 6 cut(s) 276, 566, 696, 816, 881, 903
HpySE526I ACGT 2 cut(s) 709, 765
Hsp92II CATG 9 cut(s) 34, 334, 686, 691, 916, 1017, 1072, 1310, 1406
HspAI GCGC 1 cut(s) 19
Kpn2I TCCGGA 1 cut(s) 70
KspAI GTTAAC 1 cut(s) 219
Kzo9I GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 2 cut(s) 266, 1298
Lsp1109I GCAGC 2 cut(s) 1038, 1336
LweI GCATC 4 cut(s) 547, 763, 775, 1351
MaeI CTAG 1 cut(s) 281
MaeII ACGT 2 cut(s) 709, 765
MaeIII GTNAC 2 cut(s) 40, 728
MalI GATC 6 cut(s) 144, 504, 692, 1118, 1131, 1191
MbiI CCGCTC 1 cut(s) 1301
MboI GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
MboII GAAGA 7 cut(s) 243, 308, 664, 974, 1096, 1111, 1352
MflI RGATCY 1 cut(s) 1116
MhlI GDGCHC 1 cut(s) 1339
MlsI TGGCCA 1 cut(s) 29
MluCI AATT 4 cut(s) 596, 1089, 1104, 1195
MluNI TGGCCA 1 cut(s) 29
MlyI GAGTC 2 cut(s) 362, 1072
MmeI TCCRAC 3 cut(s) 423, 1100, 1225
Mox20I TGGCCA 1 cut(s) 29
Mph1103I ATGCAT 2 cut(s) 754, 1404
MroI TCCGGA 1 cut(s) 70
MscI TGGCCA 1 cut(s) 29
MseI TTAA 4 cut(s) 218, 324, 888, 1037
MslI CAYNNNNRTG 3 cut(s) 35, 917, 1067
Msp20I TGGCCA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 659
MspI CCGG 4 cut(s) 71, 374, 485, 801
MspR9I CCNGG 3 cut(s) 99, 243, 485
Mva1269I GAATGC 1 cut(s) 160
MvaI CCWGG 2 cut(s) 99, 243
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 3 cut(s) 986, 1307, 1375
NciI CCSGG 1 cut(s) 485
NcoI CCATGG 1 cut(s) 30
NdeI CATATG 2 cut(s) 748, 1216
NdeII GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
NlaIII CATG 9 cut(s) 34, 334, 686, 691, 916, 1017, 1072, 1310, 1406
NlaIV GGNNCC 1 cut(s) 1229
NmuCI GTSAC 2 cut(s) 40, 728
NsiI ATGCAT 2 cut(s) 754, 1404
NspI RCATGY 1 cut(s) 1072
PaqCI CACCTGC 1 cut(s) 40
PciSI GCTCTTC 1 cut(s) 987
PctI GAATGC 1 cut(s) 160
PfeI GAWTC 4 cut(s) 562, 625, 721, 1136
PflMI CCANNNNNTGG 1 cut(s) 336
PfoI TCCNGGA 1 cut(s) 483
PkrI GCNGC 4 cut(s) 439, 1028, 1303, 1326
PleI GAGTC 2 cut(s) 362, 1072
PpsI GAGTC 2 cut(s) 362, 1072
Psp124BI GAGCTC 1 cut(s) 1339
Psp6I CCWGG 2 cut(s) 97, 241
PspFI CCCAGC 2 cut(s) 422, 1251
PspGI CCWGG 2 cut(s) 97, 241
PspN4I GGNNCC 1 cut(s) 1229
PspPI GGNCC 3 cut(s) 239, 284, 1254
PsuI RGATCY 1 cut(s) 1116
PvuII CAGCTG 1 cut(s) 659
RsaI GTAC 4 cut(s) 128, 712, 972, 1319
RsaNI GTAC 4 cut(s) 127, 711, 971, 1318
RseI CAYNNNNRTG 3 cut(s) 35, 917, 1067
SacI GAGCTC 1 cut(s) 1339
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 4 cut(s) 218, 324, 888, 1037
SatI GCNGC 4 cut(s) 438, 1027, 1302, 1325
Sau3AI GATC 6 cut(s) 142, 502, 690, 1116, 1129, 1189
Sau96I GGNCC 3 cut(s) 239, 284, 1254
SchI GAGTC 2 cut(s) 362, 1072
ScrFI CCNGG 3 cut(s) 99, 243, 485
SduI GDGCHC 1 cut(s) 1339
SfaNI GCATC 4 cut(s) 547, 763, 775, 1351
SfcI CTRYAG 1 cut(s) 1140
SinI GGWCC 2 cut(s) 239, 284
SmiMI CAYNNNNRTG 3 cut(s) 35, 917, 1067
SmlI CTYRAG 1 cut(s) 583
SmoI CTYRAG 1 cut(s) 583
Sse9I AATT 4 cut(s) 596, 1089, 1104, 1195
SsiI CCGC 4 cut(s) 437, 994, 1301, 1310
SspMI CTAG 1 cut(s) 281
SstI GAGCTC 1 cut(s) 1339
StyD4I CCNGG 3 cut(s) 97, 241, 483
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 6 cut(s) 46, 126, 216, 948, 1210, 1291
TaiI ACGT 2 cut(s) 712, 768
TaqI TCGA 4 cut(s) 147, 474, 1053, 1260
TasI AATT 4 cut(s) 596, 1089, 1104, 1195
TauI GCSGC 2 cut(s) 440, 1304
TfiI GAWTC 4 cut(s) 562, 625, 721, 1136
Tru1I TTAA 4 cut(s) 218, 324, 888, 1037
Tru9I TTAA 4 cut(s) 218, 324, 888, 1037
TscAI CASTG 6 cut(s) 51, 280, 520, 820, 1129, 1296
TseFI GTSAC 2 cut(s) 40, 728
TseI GCWGC 2 cut(s) 1026, 1324
Tsp45I GTSAC 2 cut(s) 40, 728
TspDTI ATGAA 2 cut(s) 947, 1002
TspGWI ACGGA 1 cut(s) 782
TspRI CASTG 6 cut(s) 51, 280, 520, 820, 1129, 1296
Van91I CCANNNNNTGG 1 cut(s) 336
VpaK11BI GGWCC 2 cut(s) 239, 284
XapI RAATTY 2 cut(s) 596, 1104
XceI RCATGY 1 cut(s) 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 1221
XmiI GTMKAC 1 cut(s) 663
XspI CTAG 1 cut(s) 281
Zsp2I ATGCAT 2 cut(s) 754, 1404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.