Rh6DG213400

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0005327
Physical Location & Seq
Forward (+)
38107479 .. 38112320
4842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG213400.1

Sequence Viewer

Length: 1080 bp
ATGTCCTGGAATAGAGCATCTAATCTCCTCTTTGTTGAGTCTCCTGCTGGAGTTGGCTGGTCATACTCAAATACAAGTTCCGATTATAATTCTGGGGATGCCTCCACTGCAAGGGATATGCACACATTCTTGTTAAAATGGTATGAGAAGTTTCCAGCTTACAAATCTAGGGAGTTGTTTCTTACCGGAGAAAGCTATGCAGGGCACTACATACCGCAATTGGCTGTTGCTATATTGGACCATAATAAACATTCAACTGGTTTCAAGTTCAATCTCAAAGGGGTTGCTATTGGGAATCCACTTCTGAAACTTGATCGTGATACACCGGCAACATATGAATATTTTTGGTCACATGGAATGATTTCTGATGAAATTGGCCTTACAATCATGAACGATTGTGATTTTGAGGATTATGAATTTGCAACGCCTCACAATGTAAGCATTGCATGCAATAATGCAATAGCTACAGCCAATAGCATAGTTGGCGAGTATATAAACAATTACGATGTGATCCTTGATGTTTGTTATCCATCTATAGTGGAGCAAGAATTGAGACTGCGGAAAGTGGCTACTAAGATAAGTGTTGGAGTTGATGTGTGTATGACATATGAACGCCGGTTTTATTTCAACCTTCCTGAGGTTCAGAAAGCCCTTCATGCAAATCGTACAAACTTGCCTTATAGCTGGGGAATGTGCAGCGGTGTTCTTAATTACAGTGATACTGATGGTAACCTAAACATGCTTCCCTTGCTGGCGAGGATAGTTAAAAATCGTATACCAGTTTGGGTTTTCAGTGGAGACCAAGATTCTGTTGTTCCACTATTGGGCTCCCGAACACTCATCCGTGAATTAGCACATGATCTAAAGTTCCAGATAACAGTGCCATATGGAGCTTGGTTTCACAAAGGCCAGGTGGGAGGTTGGGCAACGGAGTATGGAAATCTATTGACCTTTGCCACAGTAAGAGGTGCTGCTCATATGGTACCGTATGCTCAACCATCAAGATCATTGCACCTGTTCAGTTCATTTGTTCGTAATCGGAGATTGCCCAACACGACACGTCCTCCTATTGATGAATAA

Protein Analysis

359

Amino Acids

40.54

Weight (kDa)

6.42

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 1 - 346 2.3e-99 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 87
Acc65I GGTACC 1 cut(s) 982
AccB1I GGYRCC 1 cut(s) 982
AccI GTMKAC 1 cut(s) 775
AciI CCGC 3 cut(s) 215, 559, 699
AclWI GGATC 1 cut(s) 505
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 2 cut(s) 667, 984
AfiI CCNNNNNNNGG 3 cut(s) 111, 637, 824
AflIII ACRYGT 1 cut(s) 1058
AgsI TTSAA 4 cut(s) 255, 265, 271, 628
AjiI CACGTC 1 cut(s) 1061
AjnI CCWGG 2 cut(s) 5, 909
AluBI AGCT 5 cut(s) 158, 195, 464, 684, 893
AluI AGCT 5 cut(s) 158, 195, 464, 684, 893
Alw26I GTCTC 3 cut(s) 45, 547, 792
AlwI GGATC 1 cut(s) 505
AoxI GGCC 2 cut(s) 376, 907
ApeKI GCWGC 2 cut(s) 696, 971
ApoI RAATTY 1 cut(s) 416
Asp700I GAANNNNTTC 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 982
AspS9I GGNCC 1 cut(s) 238
AvaII GGWCC 1 cut(s) 238
AxyI CCTNAGG 1 cut(s) 636
BaeGI GKGCMC 1 cut(s) 207
BanI GGYRCC 1 cut(s) 982
BanII GRGCYC 1 cut(s) 830
BbvI GCAGC 2 cut(s) 708, 958
BccI CCATC 3 cut(s) 538, 719, 1006
BciT130I CCWGG 2 cut(s) 7, 911
BcoDI GTCTC 3 cut(s) 45, 547, 792
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 465, 534
BisI GCNGC 2 cut(s) 697, 972
BlsI GCNGC 2 cut(s) 698, 973
Bme1390I CCNGG 2 cut(s) 7, 911
Bme18I GGWCC 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 1061
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 829, 984
BmrFI CCNGG 2 cut(s) 7, 911
BmsI GCATC 2 cut(s) 26, 88
BpmI CTGGAG 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 792
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 4 cut(s) 579, 609, 1048, 1078
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 637, 824
Bse118I RCCGGY 2 cut(s) 325, 615
Bse1I ACTGG 2 cut(s) 262, 779
Bse21I CCTNAGG 1 cut(s) 636
Bse3DI GCAATG 2 cut(s) 441, 1007
BseBI CCWGG 2 cut(s) 7, 911
BseGI GGATG 2 cut(s) 103, 840
BseLI CCNNNNNNNGG 3 cut(s) 111, 637, 824
BseMI GCAATG 2 cut(s) 441, 1007
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 262, 779
BseRI GAGGAG 1 cut(s) 17
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 2 cut(s) 708, 958
BseYI CCCAGC 1 cut(s) 684
BsgI GTGCAG 1 cut(s) 715
BshFI GGCC 2 cut(s) 378, 909
BshNI GGYRCC 1 cut(s) 982
BsiSI CCGG 3 cut(s) 186, 326, 616
BslI CCNNNNNNNGG 3 cut(s) 111, 637, 824
BsmAI GTCTC 3 cut(s) 45, 547, 792
BsnI GGCC 2 cut(s) 378, 909
Bso31I GGTCTC 1 cut(s) 792
Bsp1286I GDGCHC 2 cut(s) 207, 830
Bsp143I GATC 4 cut(s) 313, 510, 859, 1004
BspACI CCGC 3 cut(s) 215, 559, 699
BspANI GGCC 2 cut(s) 378, 909
BspCNI CTCAG 1 cut(s) 628
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 2 cut(s) 829, 984
BspPI GGATC 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 982
BspTNI GGTCTC 1 cut(s) 792
BsrDI GCAATG 2 cut(s) 441, 1007
BsrFI RCCGGY 2 cut(s) 325, 615
BsrI ACTGG 2 cut(s) 262, 779
BssAI RCCGGY 2 cut(s) 325, 615
BssMI GATC 4 cut(s) 313, 510, 859, 1004
BssNAI GTATAC 1 cut(s) 776
Bst1107I GTATAC 1 cut(s) 776
Bst2UI CCWGG 2 cut(s) 7, 911
Bst4CI ACNGT 4 cut(s) 716, 880, 961, 987
BstAPI GCANNNNNTGC 1 cut(s) 447
BstC8I GCNNGC 2 cut(s) 448, 753
BstDEI CTNAG 2 cut(s) 573, 636
BstEII GGTNACC 1 cut(s) 728
BstENI CCTNNNNNAGG 1 cut(s) 635
BstF5I GGATG 2 cut(s) 103, 840
BstKTI GATC 4 cut(s) 316, 513, 862, 1007
BstMAI GTCTC 3 cut(s) 45, 547, 792
BstMBI GATC 4 cut(s) 313, 510, 859, 1004
BstMWI GCNNNNNNNGC 5 cut(s) 107, 447, 483, 656, 748
BstNI CCWGG 2 cut(s) 7, 911
BstNSI RCATGY 2 cut(s) 450, 742
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 2 cut(s) 5, 909
BstSFI CTRYAG 2 cut(s) 465, 534
BstSLI GKGCMC 1 cut(s) 207
BstV1I GCAGC 2 cut(s) 708, 958
BstZ17I GTATAC 1 cut(s) 776
Bsu36I CCTNAGG 1 cut(s) 636
BsuRI GGCC 2 cut(s) 378, 909
BtrI CACGTC 1 cut(s) 1061
BtsCI GGATG 2 cut(s) 103, 840
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 4 cut(s) 105, 721, 799, 885
Cac8I GCNNGC 2 cut(s) 448, 753
CciI TCATGA 1 cut(s) 387
Cfr10I RCCGGY 2 cut(s) 325, 615
Cfr13I GGNCC 1 cut(s) 238
Csp6I GTAC 2 cut(s) 666, 983
CviAII CATG 6 cut(s) 353, 388, 447, 656, 739, 857
CviQI GTAC 2 cut(s) 666, 983
DdeI CTNAG 2 cut(s) 573, 636
DpnI GATC 4 cut(s) 315, 512, 861, 1006
DpnII GATC 4 cut(s) 313, 510, 859, 1004
Eco24I GRGCYC 1 cut(s) 830
Eco31I GGTCTC 1 cut(s) 792
Eco47I GGWCC 1 cut(s) 238
Eco81I CCTNAGG 1 cut(s) 636
Eco91I GGTNACC 1 cut(s) 728
EcoNI CCTNNNNNAGG 1 cut(s) 635
EcoO65I GGTNACC 1 cut(s) 728
EcoRII CCWGG 2 cut(s) 5, 909
EcoT38I GRGCYC 1 cut(s) 830
FaeI CATG 6 cut(s) 356, 391, 450, 659, 742, 860
FatI CATG 6 cut(s) 352, 387, 446, 655, 738, 856
FauNDI CATATG 4 cut(s) 334, 607, 886, 978
FblI GTMKAC 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 697, 972
FokI GGATG 2 cut(s) 110, 827
FriOI GRGCYC 1 cut(s) 830
Fsp4HI GCNGC 2 cut(s) 697, 972
FspBI CTAG 1 cut(s) 168
GluI GCNGC 2 cut(s) 697, 972
GsaI CCCAGC 1 cut(s) 688
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 2 cut(s) 378, 909
HapII CCGG 3 cut(s) 186, 326, 616
Hin1II CATG 6 cut(s) 356, 391, 450, 659, 742, 860
HinfI GANTC 3 cut(s) 38, 295, 806
HpaII CCGG 3 cut(s) 186, 326, 616
Hpy166II GTNNAC 1 cut(s) 776
Hpy188I TCNGA 5 cut(s) 82, 306, 367, 645, 1041
Hpy188III TCNNGA 6 cut(s) 317, 388, 635, 831, 871, 1002
Hpy8I GTNNAC 1 cut(s) 776
HpyAV CCTTC 2 cut(s) 641, 662
HpyCH4III ACNGT 4 cut(s) 716, 880, 961, 987
HpyCH4IV ACGT 1 cut(s) 1060
HpyF10VI GCNNNNNNNGC 5 cut(s) 107, 447, 483, 656, 748
HpyF3I CTNAG 2 cut(s) 573, 636
HpySE526I ACGT 1 cut(s) 1060
Hsp92II CATG 6 cut(s) 356, 391, 450, 659, 742, 860
KpnI GGTACC 1 cut(s) 986
Kzo9I GATC 4 cut(s) 313, 510, 859, 1004
LmnI GCTCC 3 cut(s) 541, 833, 890
Lsp1109I GCAGC 2 cut(s) 708, 958
LweI GCATC 2 cut(s) 26, 88
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 1060
MaeIII GTNAC 2 cut(s) 348, 728
MalI GATC 4 cut(s) 315, 512, 861, 1006
MboI GATC 4 cut(s) 313, 510, 859, 1004
MfeI CAATTG 1 cut(s) 218
MhlI GDGCHC 2 cut(s) 207, 830
MluCI AATT 8 cut(s) 88, 218, 372, 416, 499, 548, 709, 848
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 565
MnlI CCTC 9 cut(s) 38, 112, 400, 438, 631, 750, 911, 959, 1074
MroXI GAANNNNTTC 1 cut(s) 361
MseI TTAA 3 cut(s) 134, 708, 765
MspA1I CMGCKG 1 cut(s) 699
MspI CCGG 3 cut(s) 186, 326, 616
MspR9I CCNGG 2 cut(s) 7, 911
MunI CAATTG 1 cut(s) 218
MvaI CCWGG 2 cut(s) 7, 911
MwoI GCNNNNNNNGC 5 cut(s) 107, 447, 483, 656, 748
NdeI CATATG 4 cut(s) 334, 607, 886, 978
NdeII GATC 4 cut(s) 313, 510, 859, 1004
NlaIII CATG 6 cut(s) 356, 391, 450, 659, 742, 860
NlaIV GGNNCC 2 cut(s) 829, 984
NmuCI GTSAC 1 cut(s) 348
NspI RCATGY 2 cut(s) 450, 742
PaeI GCATGC 1 cut(s) 450
PagI TCATGA 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 2 cut(s) 295, 806
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 2 cut(s) 698, 973
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PsiI TTATAA 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 5, 909
PspEI GGTNACC 1 cut(s) 728
PspFI CCCAGC 1 cut(s) 684
PspGI CCWGG 2 cut(s) 5, 909
PspN4I GGNNCC 2 cut(s) 829, 984
PspPI GGNCC 1 cut(s) 238
RsaI GTAC 2 cut(s) 667, 984
RsaNI GTAC 2 cut(s) 666, 983
SaqAI TTAA 3 cut(s) 134, 708, 765
SatI GCNGC 2 cut(s) 697, 972
Sau3AI GATC 4 cut(s) 313, 510, 859, 1004
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 7, 911
SduI GDGCHC 2 cut(s) 207, 830
SfaNI GCATC 2 cut(s) 26, 88
SfcI CTRYAG 2 cut(s) 465, 534
SinI GGWCC 1 cut(s) 238
SphI GCATGC 1 cut(s) 450
Sse9I AATT 8 cut(s) 88, 218, 372, 416, 499, 548, 709, 848
SsiI CCGC 3 cut(s) 215, 559, 699
SspI AATATT 1 cut(s) 341
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 5, 909
TaaI ACNGT 4 cut(s) 716, 880, 961, 987
TaiI ACGT 1 cut(s) 1063
TasI AATT 8 cut(s) 88, 218, 372, 416, 499, 548, 709, 848
TfiI GAWTC 2 cut(s) 295, 806
Tru1I TTAA 3 cut(s) 134, 708, 765
Tru9I TTAA 3 cut(s) 134, 708, 765
TscAI CASTG 4 cut(s) 112, 721, 799, 885
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 2 cut(s) 696, 971
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 7 cut(s) 351, 384, 404, 429, 624, 644, 1014
TspGWI ACGGA 2 cut(s) 833, 944
TspRI CASTG 4 cut(s) 112, 721, 799, 885
VpaK11BI GGWCC 1 cut(s) 238
XagI CCTNNNNNAGG 1 cut(s) 635
XapI RAATTY 1 cut(s) 416
XceI RCATGY 2 cut(s) 450, 742
XcmI CCANNNNNNNNNTGG 1 cut(s) 891
XmiI GTMKAC 1 cut(s) 775
XmnI GAANNNNTTC 1 cut(s) 361
XspI CTAG 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.