RLG00000013331

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0005327
Physical Location & Seq
Reverse (-)
29961877 .. 29979402
17526 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013331

Sequence Viewer

Length: 1107 bp
ATGTCCTGGAATAGAGCATCTAATCTTCTCTTTGTTGAGTCTCCTGCTGGAGTTGGATGGTCATACTCAAATACAAGTTCCGATTACAATTCTGGGGATGCCTCCACTGCAAGGGATATGCACACATTCTTGTTGAAATGGTATGAGAAGTTTCCAGCTTACAAATCTAGAGAGTTGTTTCTTACCGGAGAAAGCTATGCAGGGCACTACATACCTCAATTGGCTGTTGCTATATTGGACCATAATAAACATTCAACTGGTTTCAAGTTCAATCTCAAAGGGGTTGCTATTGGGAATCCACTTCTGAAATTTGATCGTGATGTGGCGTCAACATATGAATATTTTTGGTCACATGGAATGATTTCTGATGAAATTGGCCTTACAATCATGAATGATTGTGATTTTGAGGATTTTCCTCACAATGTAAGCATTGCATGCATTAATGCAATTGCTGCAGCCAATAGCATAGTTGGCGAGTATATAGACAATTACGATGTGATCCTTGATGTTTGTTATCCATCTATAGTGGAGCAAGAATTGAGACTGCGGAAAATGGCTACTAAGATAAGTGTTGGAGTTGATGTGTGTATGACATATGAACGCCGGTTTTATTTCAACCTTCCTGAGGTTCAGAAAGCCCTTCATGCAAATCGTACAAACTTGCCTTATAGCTGGGGAATGTGCAGCGGTGTTCTTAATTACAGTGATACTGATTTTAACCTAAACATGCTTCCCTTGCTGGCGAGGATGGTTAAAAATCATATACCAGTTTGGGTTTTCAGTGGAGACCAAGATTCTGTTGTTCCACTATTGGGCTCCCGAACACTCATCCGTGAATTAGCAAATGATCTAAAGTTCCAGATAACAGTGCCATATGGAGCTTGGTTTCACAAAGGCCAGGTGGGAGGTTGGGCAACGGAGTATGGAAATCTATTGACCTTTGCCACAGTAAGAGGTGCTGCTCATATGGTACCCTATGCTCAACCATCAAGATCTTTGCACCTGTTCAGTTCATTTGTTTGTAATAGGAGATTGCCCAACACGACACGTCCTCCCATTGATGAAAGAAGTAACATAGAAATGGTATGCTTCCGTGGCAAGATTTAG

Protein Analysis

369

Amino Acids

41.64

Weight (kDa)

6.41

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 1 - 340 1.1e-99 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 970
AciI CCGC 2 cut(s) 547, 687
AclWI GGATC 1 cut(s) 493
AcsI RAATTY 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 326
AfaI GTAC 2 cut(s) 655, 972
AfiI CCNNNNNNNGG 3 cut(s) 111, 625, 812
AflIII ACRYGT 1 cut(s) 1046
AgsI TTSAA 5 cut(s) 136, 255, 265, 271, 616
AjiI CACGTC 1 cut(s) 1049
AjnI CCWGG 2 cut(s) 5, 897
AluBI AGCT 4 cut(s) 158, 195, 672, 881
AluI AGCT 4 cut(s) 158, 195, 672, 881
Alw26I GTCTC 3 cut(s) 45, 535, 780
AlwI GGATC 1 cut(s) 493
AoxI GGCC 2 cut(s) 376, 895
ApeKI GCWGC 4 cut(s) 452, 455, 684, 959
ApoI RAATTY 1 cut(s) 308
AseI ATTAAT 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 970
AspS9I GGNCC 1 cut(s) 238
AvaII GGWCC 1 cut(s) 238
AxyI CCTNAGG 1 cut(s) 624
BaeGI GKGCMC 1 cut(s) 207
BanI GGYRCC 1 cut(s) 970
BanII GRGCYC 1 cut(s) 818
BbvI GCAGC 4 cut(s) 439, 467, 696, 946
BccI CCATC 4 cut(s) 51, 526, 742, 994
BciT130I CCWGG 2 cut(s) 7, 899
BcoDI GTCTC 3 cut(s) 45, 535, 780
BfaI CTAG 1 cut(s) 168
BfmI CTRYAG 2 cut(s) 453, 522
BglII AGATCT 1 cut(s) 992
BisI GCNGC 4 cut(s) 453, 456, 685, 960
BlsI GCNGC 4 cut(s) 454, 457, 686, 961
Bme1390I CCNGG 2 cut(s) 7, 899
Bme18I GGWCC 1 cut(s) 238
BmgBI CACGTC 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 817, 972
BmrFI CCNGG 2 cut(s) 7, 899
BmsI GCATC 2 cut(s) 26, 88
BpmI CTGGAG 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 1093
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 4 cut(s) 567, 597, 1036, 1066
Bsc4I CCNNNNNNNGG 3 cut(s) 111, 625, 812
Bse118I RCCGGY 1 cut(s) 603
Bse1I ACTGG 2 cut(s) 262, 767
Bse21I CCTNAGG 1 cut(s) 624
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 7, 899
BseDI CCNNGG 1 cut(s) 1093
BseGI GGATG 4 cut(s) 62, 103, 753, 828
BseLI CCNNNNNNNGG 3 cut(s) 111, 625, 812
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 615
BseNI ACTGG 2 cut(s) 262, 767
BseSI GKGCMC 1 cut(s) 207
BseXI GCAGC 4 cut(s) 439, 467, 696, 946
BseYI CCCAGC 1 cut(s) 672
BsgI GTGCAG 1 cut(s) 703
BshFI GGCC 2 cut(s) 378, 897
BshNI GGYRCC 1 cut(s) 970
BsiSI CCGG 2 cut(s) 186, 604
BslI CCNNNNNNNGG 3 cut(s) 111, 625, 812
BsmAI GTCTC 3 cut(s) 45, 535, 780
BsnI GGCC 2 cut(s) 378, 897
Bso31I GGTCTC 1 cut(s) 780
Bsp1286I GDGCHC 2 cut(s) 207, 818
Bsp143I GATC 4 cut(s) 313, 498, 847, 992
BspACI CCGC 2 cut(s) 547, 687
BspANI GGCC 2 cut(s) 378, 897
BspCNI CTCAG 1 cut(s) 616
BspHI TCATGA 1 cut(s) 387
BspLI GGNNCC 2 cut(s) 817, 972
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 1 cut(s) 493
BspT107I GGYRCC 1 cut(s) 970
BspTNI GGTCTC 1 cut(s) 780
BsrDI GCAATG 1 cut(s) 429
BsrFI RCCGGY 1 cut(s) 603
BsrI ACTGG 2 cut(s) 262, 767
BssAI RCCGGY 1 cut(s) 603
BssECI CCNNGG 1 cut(s) 1093
BssMI GATC 4 cut(s) 313, 498, 847, 992
BssNI GRCGYC 1 cut(s) 326
Bst2UI CCWGG 2 cut(s) 7, 899
Bst4CI ACNGT 3 cut(s) 704, 868, 949
BstACI GRCGYC 1 cut(s) 326
BstAPI GCANNNNNTGC 2 cut(s) 435, 452
BstC8I GCNNGC 2 cut(s) 436, 741
BstDEI CTNAG 2 cut(s) 561, 624
BstDSI CCRYGG 1 cut(s) 1093
BstENI CCTNNNNNAGG 1 cut(s) 623
BstF5I GGATG 4 cut(s) 62, 103, 753, 828
BstKTI GATC 4 cut(s) 316, 501, 850, 995
BstMAI GTCTC 3 cut(s) 45, 535, 780
BstMBI GATC 4 cut(s) 313, 498, 847, 992
BstMWI GCNNNNNNNGC 7 cut(s) 107, 435, 452, 471, 644, 736, 1095
BstNI CCWGG 2 cut(s) 7, 899
BstNSI RCATGY 2 cut(s) 438, 730
BstSCI CCNGG 2 cut(s) 5, 897
BstSFI CTRYAG 2 cut(s) 453, 522
BstSLI GKGCMC 1 cut(s) 207
BstV1I GCAGC 4 cut(s) 439, 467, 696, 946
BstX2I RGATCY 1 cut(s) 992
BstYI RGATCY 1 cut(s) 992
Bsu36I CCTNAGG 1 cut(s) 624
BsuRI GGCC 2 cut(s) 378, 897
BtgI CCRYGG 1 cut(s) 1093
BtrI CACGTC 1 cut(s) 1049
BtsCI GGATG 4 cut(s) 62, 103, 753, 828
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 4 cut(s) 105, 709, 787, 873
Cac8I GCNNGC 2 cut(s) 436, 741
CciI TCATGA 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 603
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 1 cut(s) 315
Csp6I GTAC 2 cut(s) 654, 971
CviAII CATG 5 cut(s) 353, 388, 435, 644, 727
CviQI GTAC 2 cut(s) 654, 971
DdeI CTNAG 2 cut(s) 561, 624
DpnI GATC 4 cut(s) 315, 500, 849, 994
DpnII GATC 4 cut(s) 313, 498, 847, 992
Eco24I GRGCYC 1 cut(s) 818
Eco31I GGTCTC 1 cut(s) 780
Eco47I GGWCC 1 cut(s) 238
Eco81I CCTNAGG 1 cut(s) 624
EcoNI CCTNNNNNAGG 1 cut(s) 623
EcoRII CCWGG 2 cut(s) 5, 897
EcoT22I ATGCAT 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 818
FaeI CATG 5 cut(s) 356, 391, 438, 647, 730
FatI CATG 5 cut(s) 352, 387, 434, 643, 726
FauNDI CATATG 4 cut(s) 334, 595, 874, 966
Fnu4HI GCNGC 4 cut(s) 453, 456, 685, 960
FokI GGATG 4 cut(s) 69, 110, 760, 815
FriOI GRGCYC 1 cut(s) 818
Fsp4HI GCNGC 4 cut(s) 453, 456, 685, 960
FspBI CTAG 1 cut(s) 168
GluI GCNGC 4 cut(s) 453, 456, 685, 960
GsaI CCCAGC 1 cut(s) 676
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 2 cut(s) 378, 897
HapII CCGG 2 cut(s) 186, 604
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 1 cut(s) 326
Hin1II CATG 5 cut(s) 356, 391, 438, 647, 730
HincII GTYRAC 1 cut(s) 330
HindII GTYRAC 1 cut(s) 330
HinfI GANTC 3 cut(s) 38, 295, 794
HpaII CCGG 2 cut(s) 186, 604
Hpy166II GTNNAC 1 cut(s) 330
Hpy188I TCNGA 4 cut(s) 82, 306, 367, 633
Hpy188III TCNNGA 7 cut(s) 168, 317, 388, 623, 819, 859, 990
Hpy8I GTNNAC 1 cut(s) 330
HpyAV CCTTC 2 cut(s) 629, 650
HpyCH4III ACNGT 3 cut(s) 704, 868, 949
HpyCH4IV ACGT 1 cut(s) 1048
HpyF10VI GCNNNNNNNGC 7 cut(s) 107, 435, 452, 471, 644, 736, 1095
HpyF3I CTNAG 2 cut(s) 561, 624
HpySE526I ACGT 1 cut(s) 1048
Hsp92I GRCGYC 1 cut(s) 326
Hsp92II CATG 5 cut(s) 356, 391, 438, 647, 730
KpnI GGTACC 1 cut(s) 974
Kzo9I GATC 4 cut(s) 313, 498, 847, 992
LmnI GCTCC 3 cut(s) 529, 821, 878
Lsp1109I GCAGC 4 cut(s) 439, 467, 696, 946
LweI GCATC 2 cut(s) 26, 88
MaeI CTAG 1 cut(s) 168
MaeII ACGT 1 cut(s) 1048
MaeIII GTNAC 2 cut(s) 348, 1070
MalI GATC 4 cut(s) 315, 500, 849, 994
MboI GATC 4 cut(s) 313, 498, 847, 992
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 2 cut(s) 218, 447
MflI RGATCY 1 cut(s) 992
MhlI GDGCHC 2 cut(s) 207, 818
MluCI AATT 9 cut(s) 88, 218, 308, 372, 447, 487, 536, 697, 836
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 2 cut(s) 34, 553
MnlI CCTC 9 cut(s) 112, 225, 400, 426, 619, 738, 899, 947, 1062
Mph1103I ATGCAT 1 cut(s) 440
MroXI GAANNNNTTC 1 cut(s) 361
MseI TTAA 4 cut(s) 441, 696, 717, 753
MslI CAYNNNNRTG 1 cut(s) 1079
MspA1I CMGCKG 1 cut(s) 687
MspI CCGG 2 cut(s) 186, 604
MspR9I CCNGG 2 cut(s) 7, 899
MunI CAATTG 2 cut(s) 218, 447
MvaI CCWGG 2 cut(s) 7, 899
MwoI GCNNNNNNNGC 7 cut(s) 107, 435, 452, 471, 644, 736, 1095
NdeI CATATG 4 cut(s) 334, 595, 874, 966
NdeII GATC 4 cut(s) 313, 498, 847, 992
NlaIII CATG 5 cut(s) 356, 391, 438, 647, 730
NlaIV GGNNCC 2 cut(s) 817, 972
NmuCI GTSAC 1 cut(s) 348
NsiI ATGCAT 1 cut(s) 440
NspI RCATGY 2 cut(s) 438, 730
PaeI GCATGC 1 cut(s) 438
PagI TCATGA 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 361
PfeI GAWTC 2 cut(s) 295, 794
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 4 cut(s) 454, 457, 686, 961
PleI GAGTC 1 cut(s) 46
PpsI GAGTC 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 441
Psp6I CCWGG 2 cut(s) 5, 897
PspFI CCCAGC 1 cut(s) 672
PspGI CCWGG 2 cut(s) 5, 897
PspN4I GGNNCC 2 cut(s) 817, 972
PspPI GGNCC 1 cut(s) 238
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 992
RsaI GTAC 2 cut(s) 655, 972
RsaNI GTAC 2 cut(s) 654, 971
RseI CAYNNNNRTG 1 cut(s) 1079
SaqAI TTAA 4 cut(s) 441, 696, 717, 753
SatI GCNGC 4 cut(s) 453, 456, 685, 960
Sau3AI GATC 4 cut(s) 313, 498, 847, 992
Sau96I GGNCC 1 cut(s) 238
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 2 cut(s) 7, 899
SduI GDGCHC 2 cut(s) 207, 818
SfaNI GCATC 2 cut(s) 26, 88
SfcI CTRYAG 2 cut(s) 453, 522
SinI GGWCC 1 cut(s) 238
SmiMI CAYNNNNRTG 1 cut(s) 1079
SphI GCATGC 1 cut(s) 438
Sse9I AATT 9 cut(s) 88, 218, 308, 372, 447, 487, 536, 697, 836
SsiI CCGC 2 cut(s) 547, 687
SspI AATATT 1 cut(s) 341
SspMI CTAG 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 5, 897
TaaI ACNGT 3 cut(s) 704, 868, 949
TaiI ACGT 1 cut(s) 1051
TasI AATT 9 cut(s) 88, 218, 308, 372, 447, 487, 536, 697, 836
TfiI GAWTC 2 cut(s) 295, 794
Tru1I TTAA 4 cut(s) 441, 696, 717, 753
Tru9I TTAA 4 cut(s) 441, 696, 717, 753
TscAI CASTG 4 cut(s) 112, 709, 787, 873
TseFI GTSAC 1 cut(s) 348
TseI GCWGC 4 cut(s) 452, 455, 684, 959
Tsp45I GTSAC 1 cut(s) 348
TspDTI ATGAA 7 cut(s) 351, 384, 404, 612, 632, 1002, 1077
TspGWI ACGGA 3 cut(s) 821, 932, 1082
TspRI CASTG 4 cut(s) 112, 709, 787, 873
VpaK11BI GGWCC 1 cut(s) 238
VspI ATTAAT 1 cut(s) 441
XagI CCTNNNNNAGG 1 cut(s) 623
XapI RAATTY 1 cut(s) 308
XbaI TCTAGA 1 cut(s) 167
XceI RCATGY 2 cut(s) 438, 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 879
XmnI GAANNNNTTC 1 cut(s) 361
XspI CTAG 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.