FvH4_2g14630

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0005327
Physical Location & Seq
Forward (+)
12845272 .. 12848389
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14630.t1

Sequence Viewer

Length: 963 bp
ATGCACACATTCTTGTTGAAATGGTATGAGAAGTTTCCAGCTTACAAGTCTAGAGCGCTGTTCCTTACCGGAGAAAGCTATGCAGGGCACTACATACCACAATTGGCTGTTGCTATATTGGATCATAACAAACATTCAACTGGTTTCAAGTTCAATCTCAAGGGGGTTGCTATTGGGAATCCACTTCTGAGACTTGATCGTGATGTACCGGCAACATATGAATTCTTTTGGTCACATGGAATGATTTCTGATGAAATTGGTCTTACAATCATGAACGATTGCGATTTTGATGATTATGAATTTGCAATCCCTCACAACGTAAGTGTAGCATGCAACAATGCAATAGCTACAGCCAATAGCATAGTTGGGGACTATATAAACAATTACGACGTGATTCTTGATGTTTGTTATCCATCTATAGTGGAGCAAGAACTGAGACTGCGGAAAACGGCTACTAAGATAAGTGTTGGAGTAGATGTGTGTATGACATATGAGCGACGGTTTTATTTCAACCTTCCTGAGGTTCAGAAAGCCCTTCATGCAAATCGTACAAACTTGCCTTATAGCTGGAGTATGTGCAGCAATGTTCTTAATTACAGTGATACTGATGGTAACCTAAACATGCTTCCCTTGCTGGCAAGGATAGTTAAAAATCATATACCACTTTGGGTTTTCAGTGGAGACGAAGATTCTGTTGTTCCACTATTGGGCTCCCGGACACTCATCCGTGAACTAGCACATGATCTAAAGTTCAAGATAACAGTGCCCTATGGAGCTTGGTTTCACAAAGGCCAGGTTGGAGGGTGGGCAACGGAGTATGGAAATCTGTTGACGTTTGCCACAGTAAGAGGCGCTGCACATATGGTACCCTATGCTCAACCATCAAGAGCATTGCACCTGTTCAGTTCATTTGTTCGTAATCGTAGATTGCCCAACACAACACGTCCTCCCATTGATGAATAA

Protein Analysis

321

Amino Acids

36.35

Weight (kDa)

6.86

Isoelectric Point (pI)

38.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 1 - 307 1.1e-78 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 865
AccB1I GGYRCC 1 cut(s) 865
AciI CCGC 1 cut(s) 442
AclWI GGATC 1 cut(s) 129
AcsI RAATTY 2 cut(s) 221, 299
AfaI GTAC 3 cut(s) 207, 550, 867
AfeI AGCGCT 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 520, 707
AflIII ACRYGT 1 cut(s) 941
AgsI TTSAA 6 cut(s) 19, 138, 148, 154, 511, 754
AjiI CACGTC 2 cut(s) 391, 944
AjnI CCWGG 1 cut(s) 792
AluBI AGCT 5 cut(s) 41, 78, 347, 567, 776
AluI AGCT 5 cut(s) 41, 78, 347, 567, 776
Alw26I GTCTC 3 cut(s) 184, 430, 675
AlwI GGATC 1 cut(s) 129
Aor51HI AGCGCT 1 cut(s) 57
AoxI GGCC 1 cut(s) 790
ApeKI GCWGC 2 cut(s) 579, 854
ApoI RAATTY 2 cut(s) 221, 299
Asp700I GAANNNNTTC 1 cut(s) 244
Asp718I GGTACC 1 cut(s) 865
AspLEI GCGC 2 cut(s) 58, 854
AsuC2I CCSGG 1 cut(s) 715
AxyI CCTNAGG 1 cut(s) 519
BaeGI GKGCMC 2 cut(s) 90, 768
BanI GGYRCC 1 cut(s) 865
BanII GRGCYC 1 cut(s) 713
BbvI GCAGC 2 cut(s) 591, 841
BccI CCATC 3 cut(s) 421, 602, 889
BceAI ACGGC 1 cut(s) 465
BciT130I CCWGG 1 cut(s) 794
BcnI CCSGG 1 cut(s) 715
BcoDI GTCTC 3 cut(s) 184, 430, 675
BfaI CTAG 2 cut(s) 51, 734
BfmI CTRYAG 2 cut(s) 348, 417
BfoI RGCGCY 2 cut(s) 59, 855
BisI GCNGC 2 cut(s) 580, 855
BlsI GCNGC 2 cut(s) 581, 856
Bme1390I CCNGG 2 cut(s) 715, 794
BmgBI CACGTC 2 cut(s) 391, 944
BmiI GGNNCC 2 cut(s) 712, 867
BmrFI CCNGG 2 cut(s) 715, 794
BpmI CTGGAG 1 cut(s) 589
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 715
BsaWI WCCGGW 1 cut(s) 68
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 931, 961
Bsc4I CCNNNNNNNGG 2 cut(s) 520, 707
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 145
Bse21I CCTNAGG 1 cut(s) 519
Bse3DI GCAATG 2 cut(s) 589, 890
BseBI CCWGG 1 cut(s) 794
BseGI GGATG 1 cut(s) 723
BseLI CCNNNNNNNGG 2 cut(s) 520, 707
BseMI GCAATG 2 cut(s) 589, 890
BseMII CTCAG 3 cut(s) 179, 425, 510
BseNI ACTGG 1 cut(s) 145
BseSI GKGCMC 2 cut(s) 90, 768
BseXI GCAGC 2 cut(s) 591, 841
BsgI GTGCAG 2 cut(s) 598, 840
BshFI GGCC 1 cut(s) 792
BshNI GGYRCC 1 cut(s) 865
BsiSI CCGG 3 cut(s) 69, 209, 715
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 2 cut(s) 520, 707
BsmAI GTCTC 3 cut(s) 184, 430, 675
BsmBI CGTCTC 1 cut(s) 675
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 1 cut(s) 792
Bsp1286I GDGCHC 3 cut(s) 90, 713, 768
Bsp143I GATC 3 cut(s) 121, 196, 742
BspACI CCGC 1 cut(s) 442
BspANI GGCC 1 cut(s) 792
BspCNI CTCAG 3 cut(s) 180, 426, 511
BspHI TCATGA 1 cut(s) 270
BspLI GGNNCC 2 cut(s) 712, 867
BspPI GGATC 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 865
BsrDI GCAATG 2 cut(s) 589, 890
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 1 cut(s) 145
BssAI RCCGGY 1 cut(s) 208
BssMI GATC 3 cut(s) 121, 196, 742
Bst2UI CCWGG 1 cut(s) 794
Bst4CI ACNGT 4 cut(s) 501, 599, 763, 844
BstC8I GCNNGC 2 cut(s) 331, 636
BstDEI CTNAG 4 cut(s) 188, 434, 456, 519
BstEII GGTNACC 1 cut(s) 611
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 1 cut(s) 723
BstH2I RGCGCY 2 cut(s) 59, 855
BstHHI GCGC 2 cut(s) 58, 854
BstKTI GATC 3 cut(s) 124, 199, 745
BstMAI GTCTC 3 cut(s) 184, 430, 675
BstMBI GATC 3 cut(s) 121, 196, 742
BstMWI GCNNNNNNNGC 2 cut(s) 539, 631
BstNI CCWGG 1 cut(s) 794
BstNSI RCATGY 2 cut(s) 333, 625
BstPI GGTNACC 1 cut(s) 611
BstSCI CCNGG 2 cut(s) 713, 792
BstSFI CTRYAG 2 cut(s) 348, 417
BstSLI GKGCMC 2 cut(s) 90, 768
BstV1I GCAGC 2 cut(s) 591, 841
Bsu36I CCTNAGG 1 cut(s) 519
BsuRI GGCC 1 cut(s) 792
BtrI CACGTC 2 cut(s) 391, 944
BtsCI GGATG 1 cut(s) 723
BtsIMutI CAGTG 3 cut(s) 604, 682, 768
Cac8I GCNNGC 2 cut(s) 331, 636
CciI TCATGA 1 cut(s) 270
CfoI GCGC 2 cut(s) 58, 854
Cfr10I RCCGGY 1 cut(s) 208
Csp6I GTAC 3 cut(s) 206, 549, 866
CviAII CATG 6 cut(s) 236, 271, 330, 539, 622, 740
CviQI GTAC 3 cut(s) 206, 549, 866
DdeI CTNAG 4 cut(s) 188, 434, 456, 519
DpnI GATC 3 cut(s) 123, 198, 744
DpnII GATC 3 cut(s) 121, 196, 742
Eco24I GRGCYC 1 cut(s) 713
Eco47III AGCGCT 1 cut(s) 57
Eco81I CCTNAGG 1 cut(s) 519
Eco91I GGTNACC 1 cut(s) 611
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoO65I GGTNACC 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 221
EcoRII CCWGG 1 cut(s) 792
EcoT38I GRGCYC 1 cut(s) 713
Esp3I CGTCTC 1 cut(s) 675
FaeI CATG 6 cut(s) 239, 274, 333, 542, 625, 743
FaqI GGGAC 1 cut(s) 383
FatI CATG 6 cut(s) 235, 270, 329, 538, 621, 739
FauNDI CATATG 3 cut(s) 217, 490, 861
Fnu4HI GCNGC 2 cut(s) 580, 855
FokI GGATG 1 cut(s) 710
FriOI GRGCYC 1 cut(s) 713
Fsp4HI GCNGC 2 cut(s) 580, 855
FspBI CTAG 2 cut(s) 51, 734
GlaI GCGC 2 cut(s) 57, 853
GluI GCNGC 2 cut(s) 580, 855
GsuI CTGGAG 1 cut(s) 589
HaeII RGCGCY 2 cut(s) 59, 855
HaeIII GGCC 1 cut(s) 792
HapII CCGG 3 cut(s) 69, 209, 715
HhaI GCGC 2 cut(s) 58, 854
Hin1II CATG 6 cut(s) 239, 274, 333, 542, 625, 743
Hin6I GCGC 2 cut(s) 56, 852
HinP1I GCGC 2 cut(s) 56, 852
HincII GTYRAC 1 cut(s) 831
HindII GTYRAC 1 cut(s) 831
HinfI GANTC 3 cut(s) 178, 394, 689
HpaII CCGG 3 cut(s) 69, 209, 715
Hpy166II GTNNAC 2 cut(s) 731, 831
Hpy188I TCNGA 3 cut(s) 189, 250, 528
Hpy188III TCNNGA 7 cut(s) 51, 200, 271, 398, 518, 754, 885
Hpy8I GTNNAC 2 cut(s) 731, 831
Hpy99I CGWCG 2 cut(s) 392, 501
HpyAV CCTTC 2 cut(s) 524, 545
HpyCH4III ACNGT 4 cut(s) 501, 599, 763, 844
HpyCH4IV ACGT 4 cut(s) 318, 390, 833, 943
HpyCH4V TGCA 9 cut(s) 4, 83, 305, 333, 341, 542, 579, 857, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 631
HpyF3I CTNAG 4 cut(s) 188, 434, 456, 519
HpySE526I ACGT 4 cut(s) 318, 390, 833, 943
Hsp92II CATG 6 cut(s) 239, 274, 333, 542, 625, 743
HspAI GCGC 2 cut(s) 56, 852
KpnI GGTACC 1 cut(s) 869
Kzo9I GATC 3 cut(s) 121, 196, 742
LmnI GCTCC 3 cut(s) 424, 716, 773
Lsp1109I GCAGC 2 cut(s) 591, 841
MaeI CTAG 2 cut(s) 51, 734
MaeII ACGT 4 cut(s) 318, 390, 833, 943
MaeIII GTNAC 2 cut(s) 231, 611
MalI GATC 3 cut(s) 123, 198, 744
MboI GATC 3 cut(s) 121, 196, 742
MboII GAAGA 1 cut(s) 698
MfeI CAATTG 1 cut(s) 101
MhlI GDGCHC 3 cut(s) 90, 713, 768
MluCI AATT 6 cut(s) 101, 221, 255, 299, 382, 592
MmeI TCCRAC 2 cut(s) 448, 778
MnlI CCTC 5 cut(s) 321, 514, 794, 842, 957
MroXI GAANNNNTTC 1 cut(s) 244
MseI TTAA 2 cut(s) 591, 648
MspI CCGG 3 cut(s) 69, 209, 715
MspR9I CCNGG 2 cut(s) 715, 794
MunI CAATTG 1 cut(s) 101
MvaI CCWGG 1 cut(s) 794
MwoI GCNNNNNNNGC 2 cut(s) 539, 631
NciI CCSGG 1 cut(s) 715
NdeI CATATG 3 cut(s) 217, 490, 861
NdeII GATC 3 cut(s) 121, 196, 742
NlaIII CATG 6 cut(s) 239, 274, 333, 542, 625, 743
NlaIV GGNNCC 2 cut(s) 712, 867
NmuCI GTSAC 1 cut(s) 231
NspI RCATGY 2 cut(s) 333, 625
PaeI GCATGC 1 cut(s) 333
PagI TCATGA 1 cut(s) 270
PdmI GAANNNNTTC 1 cut(s) 244
PfeI GAWTC 3 cut(s) 178, 394, 689
PfoI TCCNGGA 1 cut(s) 713
PkrI GCNGC 2 cut(s) 581, 856
Psp6I CCWGG 1 cut(s) 792
PspEI GGTNACC 1 cut(s) 611
PspGI CCWGG 1 cut(s) 792
PspN4I GGNNCC 2 cut(s) 712, 867
RsaI GTAC 3 cut(s) 207, 550, 867
RsaNI GTAC 3 cut(s) 206, 549, 866
SaqAI TTAA 2 cut(s) 591, 648
SatI GCNGC 2 cut(s) 580, 855
Sau3AI GATC 3 cut(s) 121, 196, 742
ScrFI CCNGG 2 cut(s) 715, 794
SduI GDGCHC 3 cut(s) 90, 713, 768
SfcI CTRYAG 2 cut(s) 348, 417
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SphI GCATGC 1 cut(s) 333
Sse9I AATT 6 cut(s) 101, 221, 255, 299, 382, 592
SsiI CCGC 1 cut(s) 442
SspMI CTAG 2 cut(s) 51, 734
StyD4I CCNGG 2 cut(s) 713, 792
TaaI ACNGT 4 cut(s) 501, 599, 763, 844
TaiI ACGT 4 cut(s) 321, 393, 836, 946
TasI AATT 6 cut(s) 101, 221, 255, 299, 382, 592
TfiI GAWTC 3 cut(s) 178, 394, 689
Tru1I TTAA 2 cut(s) 591, 648
Tru9I TTAA 2 cut(s) 591, 648
TscAI CASTG 3 cut(s) 604, 682, 768
TseFI GTSAC 1 cut(s) 231
TseI GCWGC 2 cut(s) 579, 854
Tsp45I GTSAC 1 cut(s) 231
TspDTI ATGAA 6 cut(s) 234, 267, 287, 312, 527, 897
TspGWI ACGGA 2 cut(s) 716, 827
TspRI CASTG 3 cut(s) 604, 682, 768
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 2 cut(s) 221, 299
XbaI TCTAGA 1 cut(s) 50
XceI RCATGY 2 cut(s) 333, 625
XmnI GAANNNNTTC 1 cut(s) 244
XspI CTAG 2 cut(s) 51, 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.