AT5G42240

Belongs to the peptidase S10 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005327
Physical Location & Seq
Forward (+)
16887626 .. 16891192
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G42240.1

Sequence Viewer

Length: 1422 bp
ATGGCGAGTGTGTCGTGGCGGGCTGTGGCTGTAGCTATGGTGGTGGTGTTGCTGTCATTGCAGTGGTTTGCAAAAGGGTACCCGGAAGAAGATTTGGTGGTGAGGCTGCCAGGTCAACCAACTGTTGGGTTCAAACAATACGCAGGTTATGTCGATGTCGATGTGAAAGCTGGTCGGAGTCTGTTCTATTACTATGTGGAGGCTGTGAAACAACCTGACTCAAAGCCATTGACCCTTTGGCTCAATGGAGGTCCAGGTTGTTCTTCAATTGGTGGAGGAGCTTTCACTGAGTTGGGTCCATTTTACCCCACTGGCGATGGCCGTGGCCTTCGTGTTAACTCCATGTCTTGGAACAAAGCGTCGCACCTGCTTTTTGTGGAGTCACCTGCTGGAGTAGGATGGTCTTACTCTAATAAAAGCTCTGATTACAACACCGGGGATAAGTCTACTGCAAACGATATGCTTGTGTTCTTGTTGAGATGGTTTGAGAAGTTCCCAAAGTTGAAGTCTCGGGATCTCTTTCTTACTGGCGAAAGCTATGCAGGACATTACATACCTCAACTGGCTGATGCGATTCTCAGCTACAATTCACACTCAAGTGGATTCAAATTCAACATCAAAGGCGTTGCTATTGGGAATCCACTTCTGAAGCTTGACAGAGACAGTCCAGCTACCTACGAGTTCTTCTGGTCGCATGGCATGATCTCTGATGAACTAAAACTCACAATCACGAGTCAATGTGACTTTGATGATTACACATTTGCTAGCCCCCATAACGTAAGCACGGCCTGCAATGAAGCTATTAGTGAAACTGAAAACATTATCACTGAGTATGTCAACAACTATGATGTTCTCCTCGATGTATGTTATCCGTCTATCGTCCAGCAAGAGCTGAGGCTCAAGAAAATGGCTACTAAAATGAGCATGGGAGTGGATGTGTGTATGACCTATGAAAGACGGTTCTACTTTAATCTTCCAGAGGTACAAAAGGCGCTTCATGCGAACCGTACTCATCTGCCTTATTCATGGTCAATGTGCAGCGGCGTGTTAAACTACTCTGATATCGATGGGAACATCGACATGCTTCCGATTCTGAAGAGAATTATACTGAACAAAACTCCTATTTGGATCTTCAGTGGAGATCAAGATTCTGTAGTTCCATTCGGGGGTTCAAGAACTCTCGTAAGAGAACTTGCTCAAGATCTCAACTTCAAGACAACAGTTCCCTATGGAGCCTGGTTCCACAAAAGCCAGGTTGGAGGCTGGGCCATAGAGTATGGAAAACTACTGACGTTTGCAACGGTGAGAGGAGCGGCTCATATGGTACCGTATGCACAGCCCTCACGAGCTCTACATTTGTTCAGTAGCTTTGTAAGTGGCCGGAGATTACCCAACAACACACATTCTTCTACTGATGAATGA

Protein Analysis

473

Amino Acids

52.87

Weight (kDa)

6.6

Isoelectric Point (pI)

51.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S10 PF00450 37 - 460 5.5e-137 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 375, 394
Acc36I ACCTGC 3 cut(s) 134, 375, 394
Acc65I GGTACC 2 cut(s) 78, 1324
AccB1I GGYRCC 2 cut(s) 78, 1324
AccB7I CCANNNNNTGG 2 cut(s) 125, 348
AccBSI CCGCTC 1 cut(s) 1313
AccI GTMKAC 1 cut(s) 446
AciI CCGC 3 cut(s) 19, 1041, 1313
AclWI GGATC 2 cut(s) 522, 1136
AcoI YGGCCR 2 cut(s) 319, 1378
AcsI RAATTY 1 cut(s) 608
AcuI CTGAAG 3 cut(s) 668, 1115, 1117
AfaI GTAC 4 cut(s) 80, 984, 1009, 1326
AfiI CCNNNNNNNGG 3 cut(s) 125, 348, 1166
AgsI TTSAA 7 cut(s) 133, 267, 505, 607, 613, 1173, 1213
AjnI CCWGG 4 cut(s) 109, 253, 1235, 1251
AleI CACNNNNGTG 1 cut(s) 597
Alw21I GWGCWC 1 cut(s) 1351
Alw26I GTCTC 2 cut(s) 513, 654
AlwI GGATC 2 cut(s) 522, 1136
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 5 cut(s) 319, 325, 786, 1266, 1378
ApeKI GCWGC 2 cut(s) 106, 1038
ApoI RAATTY 1 cut(s) 608
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
Asp718I GGTACC 2 cut(s) 78, 1324
AspLEI GCGC 1 cut(s) 994
AspS9I GGNCC 3 cut(s) 251, 296, 1266
AsuC2I CCSGG 2 cut(s) 83, 436
AsuHPI GGTGA 3 cut(s) 112, 375, 1315
AsuNHI GCTAGC 1 cut(s) 764
AvaI CYCGRG 1 cut(s) 510
AvaII GGWCC 2 cut(s) 251, 296
BanI GGYRCC 2 cut(s) 78, 1324
BanII GRGCYC 1 cut(s) 1351
BauI CACGAG 2 cut(s) 730, 1344
Bbv12I GWGCWC 1 cut(s) 1351
BbvCI CCTCAGC 1 cut(s) 893
BbvI GCAGC 2 cut(s) 93, 1050
BccI CCATC 4 cut(s) 311, 393, 474, 1061
BceAI ACGGC 2 cut(s) 306, 801
BcgI CGANNNNNNTGC 2 cut(s) 521, 555
BciT130I CCWGG 4 cut(s) 111, 255, 1237, 1253
BcnI CCSGG 2 cut(s) 83, 436
BcoDI GTCTC 2 cut(s) 513, 654
BfaI CTAG 1 cut(s) 765
BfmI CTRYAG 2 cut(s) 30, 1152
BfoI RGCGCY 1 cut(s) 995
BfuAI ACCTGC 3 cut(s) 134, 375, 394
BglII AGATCT 1 cut(s) 1201
BisI GCNGC 4 cut(s) 107, 1039, 1042, 1314
BlsI GCNGC 4 cut(s) 108, 1040, 1043, 1315
Bme1390I CCNGG 6 cut(s) 83, 111, 255, 436, 1237, 1253
Bme18I GGWCC 2 cut(s) 251, 296
BmeT110I CYCGRG 1 cut(s) 510
BmgT120I GGNCC 3 cut(s) 251, 296, 1266
BmiI GGNNCC 5 cut(s) 80, 297, 1234, 1241, 1326
BmrFI CCNGG 6 cut(s) 83, 111, 255, 436, 1237, 1253
BmsI GCATC 1 cut(s) 559
BmtI GCTAGC 1 cut(s) 768
BpmI CTGGAG 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 893
BpuEI CTTGAG 3 cut(s) 580, 884, 1182
BpuMI CCSGG 2 cut(s) 83, 436
Bsa29I ATCGAT 1 cut(s) 1065
BsaJI CCNNGG 2 cut(s) 322, 435
BsaXI ACNNNNNCTCC 2 cut(s) 921, 951
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 348, 1166
Bse1I ACTGG 3 cut(s) 316, 532, 567
Bse3DI GCAATG 2 cut(s) 56, 799
BseBI CCWGG 4 cut(s) 111, 255, 1237, 1253
BseCI ATCGAT 1 cut(s) 1065
BseDI CCNNGG 2 cut(s) 322, 435
BseGI GGATG 2 cut(s) 404, 940
BseLI CCNNNNNNNGG 3 cut(s) 125, 348, 1166
BseMI GCAATG 2 cut(s) 56, 799
BseMII CTCAG 4 cut(s) 279, 592, 819, 884
BseNI ACTGG 3 cut(s) 316, 532, 567
BseRI GAGGAG 3 cut(s) 291, 845, 1323
BseXI GCAGC 2 cut(s) 93, 1050
BseYI CCCAGC 1 cut(s) 1263
BsgI GTGCAG 1 cut(s) 1057
BshFI GGCC 5 cut(s) 321, 327, 788, 1268, 1380
BshNI GGYRCC 2 cut(s) 78, 1324
BshVI ATCGAT 1 cut(s) 1065
BsiHKAI GWGCWC 1 cut(s) 1351
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 3 cut(s) 83, 435, 1381
BslI CCNNNNNNNGG 3 cut(s) 125, 348, 1166
BsmAI GTCTC 2 cut(s) 513, 654
BsnI GGCC 5 cut(s) 321, 327, 788, 1268, 1380
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 1351
Bsp143I GATC 5 cut(s) 514, 702, 1128, 1141, 1201
BspACI CCGC 3 cut(s) 19, 1041, 1313
BspANI GGCC 5 cut(s) 321, 327, 788, 1268, 1380
BspCNI CTCAG 4 cut(s) 280, 591, 820, 885
BspDI ATCGAT 1 cut(s) 1065
BspLI GGNNCC 5 cut(s) 80, 297, 1234, 1241, 1326
BspMI ACCTGC 3 cut(s) 134, 375, 394
BspOI GCTAGC 1 cut(s) 768
BspPI GGATC 2 cut(s) 522, 1136
BspT107I GGYRCC 2 cut(s) 78, 1324
BsrBI CCGCTC 1 cut(s) 1313
BsrDI GCAATG 2 cut(s) 56, 799
BsrI ACTGG 3 cut(s) 316, 532, 567
BssECI CCNNGG 2 cut(s) 322, 435
BssMI GATC 5 cut(s) 514, 702, 1128, 1141, 1201
BssSI CACGAG 2 cut(s) 730, 1344
Bst2BI CACGAG 2 cut(s) 730, 1344
Bst2UI CCWGG 4 cut(s) 111, 255, 1237, 1253
Bst4CI ACNGT 7 cut(s) 124, 665, 960, 1007, 1222, 1303, 1329
Bst6I CTCTTC 1 cut(s) 1091
BstAPI GCANNNNNTGC 1 cut(s) 789
BstC8I GCNNGC 3 cut(s) 21, 766, 790
BstDEI CTNAG 4 cut(s) 288, 578, 828, 893
BstDSI CCRYGG 1 cut(s) 322
BstF5I GGATG 2 cut(s) 404, 940
BstH2I RGCGCY 1 cut(s) 995
BstHHI GCGC 1 cut(s) 994
BstKTI GATC 5 cut(s) 517, 705, 1131, 1144, 1204
BstMAI GTCTC 2 cut(s) 513, 654
BstMBI GATC 5 cut(s) 514, 702, 1128, 1141, 1201
BstMWI GCNNNNNNNGC 3 cut(s) 58, 789, 998
BstNI CCWGG 4 cut(s) 111, 255, 1237, 1253
BstNSI RCATGY 1 cut(s) 1084
BstSCI CCNGG 6 cut(s) 81, 109, 253, 434, 1235, 1251
BstSFI CTRYAG 2 cut(s) 30, 1152
BstV1I GCAGC 2 cut(s) 93, 1050
BstX2I RGATCY 3 cut(s) 514, 1128, 1201
BstYI RGATCY 3 cut(s) 514, 1128, 1201
Bsu15I ATCGAT 1 cut(s) 1065
BsuRI GGCC 5 cut(s) 321, 327, 788, 1268, 1380
BsuTUI ATCGAT 1 cut(s) 1065
BtgI CCRYGG 1 cut(s) 322
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 404, 940
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 5 cut(s) 68, 285, 309, 825, 1141
BveI ACCTGC 3 cut(s) 134, 375, 394
Cac8I GCNNGC 3 cut(s) 21, 766, 790
CfoI GCGC 1 cut(s) 994
Cfr13I GGNCC 3 cut(s) 251, 296, 1266
ClaI ATCGAT 1 cut(s) 1065
CseI GACGC 1 cut(s) 348
Csp6I GTAC 4 cut(s) 79, 983, 1008, 1325
CviAII CATG 7 cut(s) 343, 695, 700, 925, 998, 1026, 1081
CviQI GTAC 4 cut(s) 79, 983, 1008, 1325
DdeI CTNAG 4 cut(s) 288, 578, 828, 893
DpnI GATC 5 cut(s) 516, 704, 1130, 1143, 1203
DpnII GATC 5 cut(s) 514, 702, 1128, 1141, 1201
EaeI YGGCCR 2 cut(s) 319, 1378
Eam1104I CTCTTC 1 cut(s) 1091
EarI CTCTTC 1 cut(s) 1091
Ecl136II GAGCTC 1 cut(s) 1349
Eco24I GRGCYC 1 cut(s) 1351
Eco32I GATATC 1 cut(s) 1063
Eco47I GGWCC 2 cut(s) 251, 296
Eco53kI GAGCTC 1 cut(s) 1349
Eco57I CTGAAG 3 cut(s) 668, 1115, 1117
Eco88I CYCGRG 1 cut(s) 510
EcoICRI GAGCTC 1 cut(s) 1349
EcoRII CCWGG 4 cut(s) 109, 253, 1235, 1251
EcoRV GATATC 1 cut(s) 1063
EcoT38I GRGCYC 1 cut(s) 1351
FaeI CATG 7 cut(s) 346, 698, 703, 928, 1001, 1029, 1084
FatI CATG 7 cut(s) 342, 694, 699, 924, 997, 1025, 1080
FauI CCCGC 1 cut(s) 12
FauNDI CATATG 1 cut(s) 1320
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 4 cut(s) 107, 1039, 1042, 1314
FokI GGATG 2 cut(s) 411, 947
FriOI GRGCYC 1 cut(s) 1351
Fsp4HI GCNGC 4 cut(s) 107, 1039, 1042, 1314
FspBI CTAG 1 cut(s) 765
GlaI GCGC 1 cut(s) 993
GluI GCNGC 4 cut(s) 107, 1039, 1042, 1314
GsaI CCCAGC 1 cut(s) 1267
GsuI CTGGAG 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 995
HaeIII GGCC 5 cut(s) 321, 327, 788, 1268, 1380
HapII CCGG 3 cut(s) 83, 435, 1381
HgaI GACGC 1 cut(s) 348
HhaI GCGC 1 cut(s) 994
Hin1II CATG 7 cut(s) 346, 698, 703, 928, 1001, 1029, 1084
Hin6I GCGC 1 cut(s) 992
HinP1I GCGC 1 cut(s) 992
HincII GTYRAC 3 cut(s) 116, 337, 838
HindII GTYRAC 3 cut(s) 116, 337, 838
HindIII AAGCTT 1 cut(s) 650
HinfI GANTC 9 cut(s) 178, 218, 380, 574, 603, 637, 733, 1090, 1148
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 3 cut(s) 83, 435, 1381
HphI GGTGA 3 cut(s) 112, 375, 1315
Hpy166II GTNNAC 4 cut(s) 116, 337, 447, 838
Hpy188I TCNGA 7 cut(s) 177, 424, 648, 709, 1060, 1089, 1095
Hpy188III TCNNGA 9 cut(s) 512, 730, 901, 977, 1145, 1173, 1199, 1213, 1344
Hpy8I GTNNAC 4 cut(s) 116, 337, 447, 838
Hpy99I CGWCG 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 338
HpyCH4III ACNGT 7 cut(s) 124, 665, 960, 1007, 1222, 1303, 1329
HpyCH4IV ACGT 2 cut(s) 777, 1292
HpyCH4V TGCA 8 cut(s) 61, 71, 452, 542, 792, 1038, 1298, 1334
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 789, 998
HpyF3I CTNAG 4 cut(s) 288, 578, 828, 893
HpySE526I ACGT 2 cut(s) 777, 1292
Hsp92II CATG 7 cut(s) 346, 698, 703, 928, 1001, 1029, 1084
HspAI GCGC 1 cut(s) 992
KpnI GGTACC 2 cut(s) 82, 1328
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 5 cut(s) 514, 702, 1128, 1141, 1201
LmnI GCTCC 3 cut(s) 278, 1232, 1310
Lsp1109I GCAGC 2 cut(s) 93, 1050
LweI GCATC 1 cut(s) 559
MaeI CTAG 1 cut(s) 765
MaeII ACGT 2 cut(s) 777, 1292
MaeIII GTNAC 2 cut(s) 381, 740
MalI GATC 5 cut(s) 516, 704, 1130, 1143, 1203
MbiI CCGCTC 1 cut(s) 1313
MboI GATC 5 cut(s) 514, 702, 1128, 1141, 1201
MboII GAAGA 8 cut(s) 98, 101, 255, 676, 965, 1108, 1123, 1398
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 3 cut(s) 514, 1128, 1201
MhlI GDGCHC 1 cut(s) 1351
MluCI AATT 4 cut(s) 267, 586, 608, 1101
MlyI GAGTC 4 cut(s) 187, 212, 389, 742
MmeI TCCRAC 2 cut(s) 155, 1237
MseI TTAA 3 cut(s) 336, 969, 1049
MslI CAYNNNNRTG 4 cut(s) 61, 597, 929, 1079
MspA1I CMGCKG 1 cut(s) 1041
MspI CCGG 3 cut(s) 83, 435, 1381
MspR9I CCNGG 6 cut(s) 83, 111, 255, 436, 1237, 1253
MunI CAATTG 1 cut(s) 267
MvaI CCWGG 4 cut(s) 111, 255, 1237, 1253
MwoI GCNNNNNNNGC 3 cut(s) 58, 789, 998
NciI CCSGG 2 cut(s) 83, 436
NdeI CATATG 1 cut(s) 1320
NdeII GATC 5 cut(s) 514, 702, 1128, 1141, 1201
NheI GCTAGC 1 cut(s) 764
NlaIII CATG 7 cut(s) 346, 698, 703, 928, 1001, 1029, 1084
NlaIV GGNNCC 5 cut(s) 80, 297, 1234, 1241, 1326
NmuCI GTSAC 2 cut(s) 381, 740
NspI RCATGY 1 cut(s) 1084
OliI CACNNNNGTG 1 cut(s) 597
PaqCI CACCTGC 2 cut(s) 375, 394
PfeI GAWTC 5 cut(s) 574, 603, 637, 1090, 1148
PflMI CCANNNNNTGG 2 cut(s) 125, 348
PkrI GCNGC 4 cut(s) 108, 1040, 1043, 1315
PleI GAGTC 4 cut(s) 186, 212, 388, 741
PpsI GAGTC 4 cut(s) 186, 212, 388, 741
Psp124BI GAGCTC 1 cut(s) 1351
Psp6I CCWGG 4 cut(s) 109, 253, 1235, 1251
PspFI CCCAGC 1 cut(s) 1263
PspGI CCWGG 4 cut(s) 109, 253, 1235, 1251
PspN4I GGNNCC 5 cut(s) 80, 297, 1234, 1241, 1326
PspPI GGNCC 3 cut(s) 251, 296, 1266
PsuI RGATCY 3 cut(s) 514, 1128, 1201
RsaI GTAC 4 cut(s) 80, 984, 1009, 1326
RsaNI GTAC 4 cut(s) 79, 983, 1008, 1325
RseI CAYNNNNRTG 4 cut(s) 61, 597, 929, 1079
SacI GAGCTC 1 cut(s) 1351
SaqAI TTAA 3 cut(s) 336, 969, 1049
SatI GCNGC 4 cut(s) 107, 1039, 1042, 1314
Sau3AI GATC 5 cut(s) 514, 702, 1128, 1141, 1201
Sau96I GGNCC 3 cut(s) 251, 296, 1266
SchI GAGTC 4 cut(s) 187, 212, 389, 742
ScrFI CCNGG 6 cut(s) 83, 111, 255, 436, 1237, 1253
SduI GDGCHC 1 cut(s) 1351
SfaNI GCATC 1 cut(s) 559
SfcI CTRYAG 2 cut(s) 30, 1152
SinI GGWCC 2 cut(s) 251, 296
SmiMI CAYNNNNRTG 4 cut(s) 61, 597, 929, 1079
SmlI CTYRAG 3 cut(s) 595, 899, 1197
SmoI CTYRAG 3 cut(s) 595, 899, 1197
Sse9I AATT 4 cut(s) 267, 586, 608, 1101
SsiI CCGC 3 cut(s) 19, 1041, 1313
SspMI CTAG 1 cut(s) 765
SstI GAGCTC 1 cut(s) 1351
StyD4I CCNGG 6 cut(s) 81, 109, 253, 434, 1235, 1251
TaaI ACNGT 7 cut(s) 124, 665, 960, 1007, 1222, 1303, 1329
TaiI ACGT 2 cut(s) 780, 1295
TaqI TCGA 5 cut(s) 153, 159, 858, 1065, 1077
TasI AATT 4 cut(s) 267, 586, 608, 1101
TauI GCSGC 2 cut(s) 1044, 1316
TfiI GAWTC 5 cut(s) 574, 603, 637, 1090, 1148
Tru1I TTAA 3 cut(s) 336, 969, 1049
Tru9I TTAA 3 cut(s) 336, 969, 1049
TscAI CASTG 5 cut(s) 68, 292, 316, 832, 1141
TseFI GTSAC 2 cut(s) 381, 740
TseI GCWGC 2 cut(s) 106, 1038
Tsp45I GTSAC 2 cut(s) 381, 740
TspDTI ATGAA 5 cut(s) 726, 810, 966, 986, 1014
TspGWI ACGGA 1 cut(s) 861
TspRI CASTG 5 cut(s) 68, 292, 316, 832, 1141
Van91I CCANNNNNTGG 2 cut(s) 125, 348
VpaK11BI GGWCC 2 cut(s) 251, 296
XapI RAATTY 1 cut(s) 608
XceI RCATGY 1 cut(s) 1084
XcmI CCANNNNNNNNNTGG 1 cut(s) 234
XmiI GTMKAC 1 cut(s) 446
XspI CTAG 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.