AT1G48580

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006732
Physical Location & Seq
Reverse (-)
17957942 .. 17960916
2975 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G48580.3

Sequence Viewer

Length: 1365 bp
ATGAACTCCCAAGCTGAAGTAGACCAATGGGAAGCTCTTGATCTCGGCGATTCTGAACTCCCGTCTTTTCTCCGCCCTTGTAAACGCAAATCTCCGACGAGGTCTCTGCAACCGCATGCGCAACAACAGAATCCAAAGGCGGGTTTTAATAGTAACACTAATCACCGTCCCACTCTTCGGCGCTGCTCCTCGCCGGATAAGTTTCTTGAGGAGAGTTATTCTCGCTCCTTGATTCCAGGACCTGCTGGAGTTGTTCAAGTTGCAATCCGTAGAAAGATGAATAAGGATCCTAAATCGTTTAATGAGCATGGAGAACCAATTCCAACTCAAGAGTTCTTACGTAAGGCAGCAGAAGAGCCTGATTGGGAAGACAAGGATTTCTCTGAGGACCCCTGGGTCTCTACTGTTGATTACATTCGAAGTGAAGGTTTGTTGAGCAATGGTGGAAATGCTATAGGGACACCAGTGAGCGAGATTAAGAGAAGATGTGACAGTTGGGGTAAAGTTGATCAGGTCGTGGCAATTGTCAAAACTTGCACTCCGAATGGTCTAGGTGATGTCATGGTGACTCTTAAGGATCCCACTGGAACAATTGATGCCAGTGTGCACAGAAAAGTTATCTCCGAGAGTGAGTTTGGTAGAGATATACGAGTTGGCGCAGTGGTGATACTTAAGCAGGTTGCAGTCTGCGCACCCTCACGTTCATCAACCTATCTTAACATAACACTGAAGAACATCTCCAAGGTTATCACAAAGGATACTCCTGTTTTACCAAACCAGAACGATTCTGAGATGAGTGCCAAGAATCATGTTTCTGTGAATGGTTTACCAAAGCAGAACGATTCTGAAATGAGTGGCAAGAATCTTGTTCCGGTGAATGAAAACGAGGAATATTTGCGACTGCAACCCAATGTGTTTAGTGTTGAGCAAAGCACTACTCAAGGCATCATGAACAATCTCAGACAAAATGCTAAAGGAAGCAGTGAAGCACTGCATGACATAGAAATGGTAGATATTAATCCTGCAGAGGGAAGCAAATCCTCTCCTAAAAAGGGTGTGACAAAGAATCACTGTGAAGTTAGAATGGAGCAGACTCTCTTGGGAAAACATGATTCAATCAGCCAAACAGAGCAGCAACTGTACGAAGATGTAGCTACTGAGACTGATATAGCTGATTGCATTAGACCCGCGAAACAGATCAGAAGAAGTTCTCAATCGCAGATTGATGAGCAAGAGAGTGTAATGGGGAACCCTGATGAAGTCACAACTCGAACTACAATCCACAAATCTCAATCGATGGCGTCAACAATCTCGTTGCCACAATGGACGGACGAGCAACTTGAAGAACTCTTTGCTTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

454

Amino Acids

50.45

Weight (kDa)

5.41

Isoelectric Point (pI)

61.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HROB PF15072 171 - 255 2e-29 Homologous recombination OB-fold protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
Acc16I TGCGCA 2 cut(s) 120, 691
Acc36I ACCTGC 2 cut(s) 250, 667
AccI GTMKAC 1 cut(s) 21
AccII CGCG 1 cut(s) 1190
AciI CCGC 4 cut(s) 73, 113, 140, 1188
AclWI GGATC 4 cut(s) 281, 294, 572, 585
AcuI CTGAAG 2 cut(s) 36, 749
AcyI GRCGYC 1 cut(s) 1301
AfaI GTAC 1 cut(s) 1142
AfiI CCNNNNNNNGG 4 cut(s) 140, 177, 1028, 1052
AflII CTTAAG 2 cut(s) 572, 671
AgsI TTSAA 3 cut(s) 257, 1116, 1343
AjnI CCWGG 2 cut(s) 235, 392
AluBI AGCT 4 cut(s) 14, 35, 1154, 1172
AluI AGCT 4 cut(s) 14, 35, 1154, 1172
Alw21I GWGCWC 1 cut(s) 609
Alw26I GTCTC 3 cut(s) 108, 403, 1154
Alw44I GTGCAC 1 cut(s) 605
AlwI GGATC 4 cut(s) 281, 294, 572, 585
AlwNI CAGNNNCTG 2 cut(s) 242, 1138
ApaLI GTGCAC 1 cut(s) 605
ApeKI GCWGC 3 cut(s) 183, 347, 1132
AseI ATTAAT 1 cut(s) 1017
Asp700I GAANNNNTTC 2 cut(s) 318, 1207
AspLEI GCGC 4 cut(s) 121, 183, 659, 692
AspS9I GGNCC 2 cut(s) 239, 388
AsuHPI GGTGA 5 cut(s) 155, 566, 577, 676, 886
AsuII TTCGAA 1 cut(s) 418
AvaII GGWCC 2 cut(s) 239, 388
BaeGI GKGCMC 1 cut(s) 609
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BamHI GGATCC 2 cut(s) 286, 577
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 609
BbvI GCAGC 3 cut(s) 170, 359, 1144
BccI CCATC 1 cut(s) 1291
BcgI CGANNNNNNTGC 2 cut(s) 88, 122
BciT130I CCWGG 2 cut(s) 237, 394
BciVI GTATCC 1 cut(s) 751
BclI TGATCA 1 cut(s) 508
BcoDI GTCTC 3 cut(s) 108, 403, 1154
BfaI CTAG 1 cut(s) 551
BfmI CTRYAG 2 cut(s) 453, 1023
BfoI RGCGCY 1 cut(s) 184
BfrI CTTAAG 2 cut(s) 572, 671
BfuAI ACCTGC 2 cut(s) 250, 667
BfuI GTATCC 1 cut(s) 751
BisI GCNGC 3 cut(s) 184, 348, 1133
BlsI GCNGC 3 cut(s) 185, 349, 1134
Bme1390I CCNGG 2 cut(s) 237, 394
Bme18I GGWCC 2 cut(s) 239, 388
BmgT120I GGNCC 2 cut(s) 239, 388
BmiI GGNNCC 4 cut(s) 288, 390, 579, 1250
BmrFI CCNGG 2 cut(s) 237, 394
BmsI GCATC 2 cut(s) 586, 954
BpiI GAAGAC 1 cut(s) 375
BplI GAGNNNNNCTC 2 cut(s) 205, 237
BpmI CTGGAG 1 cut(s) 267
Bpu14I TTCGAA 1 cut(s) 418
BpuEI CTTGAG 3 cut(s) 227, 312, 924
Bsa29I ATCGAT 1 cut(s) 1295
BsaAI YACGTR 1 cut(s) 341
BsaBI GATNNNNATC 1 cut(s) 1017
BsaHI GRCGYC 1 cut(s) 1301
BsaI GGTCTC 2 cut(s) 108, 403
BsaJI CCNNGG 3 cut(s) 392, 393, 741
BsaWI WCCGGW 1 cut(s) 871
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 177, 1028, 1052
Bse1I ACTGG 3 cut(s) 464, 589, 600
Bse3DI GCAATG 1 cut(s) 445
Bse8I GATNNNNATC 1 cut(s) 1017
BseBI CCWGG 2 cut(s) 237, 394
BseCI ATCGAT 1 cut(s) 1295
BseDI CCNNGG 3 cut(s) 392, 393, 741
BseJI GATNNNNATC 1 cut(s) 1017
BseLI CCNNNNNNNGG 4 cut(s) 140, 177, 1028, 1052
BseMI GCAATG 1 cut(s) 445
BseMII CTCAG 4 cut(s) 375, 780, 973, 1149
BseNI ACTGG 3 cut(s) 464, 589, 600
BseRI GAGGAG 2 cut(s) 178, 224
BseSI GKGCMC 1 cut(s) 609
BseXI GCAGC 3 cut(s) 170, 359, 1144
Bsh1236I CGCG 1 cut(s) 1190
BshVI ATCGAT 1 cut(s) 1295
BsiHKAI GWGCWC 1 cut(s) 609
BsiSI CCGG 2 cut(s) 194, 872
BslFI GGGAC 2 cut(s) 153, 472
BslI CCNNNNNNNGG 4 cut(s) 140, 177, 1028, 1052
BsmAI GTCTC 3 cut(s) 108, 403, 1154
BsmFI GGGAC 2 cut(s) 153, 472
Bso31I GGTCTC 2 cut(s) 108, 403
Bsp119I TTCGAA 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 609
Bsp143I GATC 5 cut(s) 40, 286, 508, 577, 1197
BspACI CCGC 4 cut(s) 73, 113, 140, 1188
BspCNI CTCAG 4 cut(s) 376, 781, 972, 1150
BspDI ATCGAT 1 cut(s) 1295
BspFNI CGCG 1 cut(s) 1190
BspHI TCATGA 1 cut(s) 948
BspLI GGNNCC 4 cut(s) 288, 390, 579, 1250
BspMAI CTGCAG 1 cut(s) 1027
BspMI ACCTGC 2 cut(s) 250, 667
BspPI GGATC 4 cut(s) 281, 294, 572, 585
BspQI GCTCTTC 1 cut(s) 348
BspT104I TTCGAA 1 cut(s) 418
BspTI CTTAAG 2 cut(s) 572, 671
BspTNI GGTCTC 2 cut(s) 108, 403
BsrDI GCAATG 1 cut(s) 445
BsrI ACTGG 3 cut(s) 464, 589, 600
BssECI CCNNGG 3 cut(s) 392, 393, 741
BssMI GATC 5 cut(s) 40, 286, 508, 577, 1197
BssNI GRCGYC 1 cut(s) 1301
BssT1I CCWWGG 1 cut(s) 741
Bst2UI CCWGG 2 cut(s) 237, 394
Bst4CI ACNGT 5 cut(s) 167, 406, 494, 1073, 1140
Bst6I CTCTTC 2 cut(s) 180, 348
BstACI GRCGYC 1 cut(s) 1301
BstAFI CTTAAG 2 cut(s) 572, 671
BstBAI YACGTR 1 cut(s) 341
BstBI TTCGAA 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 117
BstDEI CTNAG 4 cut(s) 384, 789, 959, 1158
BstFNI CGCG 1 cut(s) 1190
BstH2I RGCGCY 1 cut(s) 184
BstHHI GCGC 4 cut(s) 121, 183, 659, 692
BstKTI GATC 5 cut(s) 43, 289, 511, 580, 1200
BstMAI GTCTC 3 cut(s) 108, 403, 1154
BstMBI GATC 5 cut(s) 40, 286, 508, 577, 1197
BstMWI GCNNNNNNNGC 1 cut(s) 689
BstNI CCWGG 2 cut(s) 237, 394
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 235, 392
BstSFI CTRYAG 2 cut(s) 453, 1023
BstSLI GKGCMC 1 cut(s) 609
BstSNI TACGTA 1 cut(s) 341
BstUI CGCG 1 cut(s) 1190
BstV1I GCAGC 3 cut(s) 170, 359, 1144
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 286, 577
BstYI RGATCY 2 cut(s) 286, 577
Bsu15I ATCGAT 1 cut(s) 1295
BsuI GTATCC 1 cut(s) 751
BsuTUI ATCGAT 1 cut(s) 1295
BtsI GCAGTG 3 cut(s) 666, 988, 989
BtsIMutI CAGTG 8 cut(s) 471, 582, 607, 666, 725, 988, 989, 1069
BveI ACCTGC 2 cut(s) 250, 667
Cac8I GCNNGC 1 cut(s) 117
CaiI CAGNNNCTG 2 cut(s) 242, 1138
CciI TCATGA 1 cut(s) 948
CfoI GCGC 4 cut(s) 121, 183, 659, 692
Cfr13I GGNCC 2 cut(s) 239, 388
ClaI ATCGAT 1 cut(s) 1295
CseI GACGC 1 cut(s) 1290
Csp6I GTAC 1 cut(s) 1141
CviAII CATG 7 cut(s) 116, 308, 562, 809, 949, 995, 1109
CviJI RGCY 6 cut(s) 14, 35, 358, 1122, 1154, 1172
CviKI_1 RGCY 6 cut(s) 14, 35, 358, 1122, 1154, 1172
CviQI GTAC 1 cut(s) 1141
DdeI CTNAG 4 cut(s) 384, 789, 959, 1158
DpnI GATC 5 cut(s) 42, 288, 510, 579, 1199
DpnII GATC 5 cut(s) 40, 286, 508, 577, 1197
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
Eam1104I CTCTTC 2 cut(s) 180, 348
EarI CTCTTC 2 cut(s) 180, 348
EciI GGCGGA 1 cut(s) 62
Eco105I TACGTA 1 cut(s) 341
Eco130I CCWWGG 1 cut(s) 741
Eco31I GGTCTC 2 cut(s) 108, 403
Eco47I GGWCC 2 cut(s) 239, 388
Eco57I CTGAAG 2 cut(s) 36, 749
EcoO109I RGGNCCY 2 cut(s) 239, 388
EcoRII CCWGG 2 cut(s) 235, 392
EcoT14I CCWWGG 1 cut(s) 741
ErhI CCWWGG 1 cut(s) 741
FaeI CATG 7 cut(s) 119, 311, 565, 812, 952, 998, 1112
FaqI GGGAC 2 cut(s) 153, 472
FatI CATG 7 cut(s) 115, 307, 561, 808, 948, 994, 1108
FauI CCCGC 2 cut(s) 133, 1195
FbaI TGATCA 1 cut(s) 508
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 3 cut(s) 184, 348, 1133
Fsp4HI GCNGC 3 cut(s) 184, 348, 1133
FspBI CTAG 1 cut(s) 551
FspI TGCGCA 2 cut(s) 120, 691
GlaI GCGC 4 cut(s) 120, 182, 658, 691
GluI GCNGC 3 cut(s) 184, 348, 1133
GsuI CTGGAG 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 184
HapII CCGG 2 cut(s) 194, 872
HgaI GACGC 1 cut(s) 1290
HhaI GCGC 4 cut(s) 121, 183, 659, 692
Hin1I GRCGYC 1 cut(s) 1301
Hin1II CATG 7 cut(s) 119, 311, 565, 812, 952, 998, 1112
Hin6I GCGC 4 cut(s) 119, 181, 657, 690
HinP1I GCGC 4 cut(s) 119, 181, 657, 690
HincII GTYRAC 1 cut(s) 1305
HindII GTYRAC 1 cut(s) 1305
HpaII CCGG 2 cut(s) 194, 872
HphI GGTGA 5 cut(s) 155, 566, 577, 676, 886
Hpy166II GTNNAC 5 cut(s) 22, 83, 607, 827, 1305
Hpy188I TCNGA 9 cut(s) 55, 96, 385, 543, 625, 790, 847, 962, 1202
Hpy188III TCNNGA 4 cut(s) 38, 206, 329, 949
Hpy8I GTNNAC 5 cut(s) 22, 83, 607, 827, 1305
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 5 cut(s) 167, 406, 494, 1073, 1140
HpyCH4IV ACGT 2 cut(s) 340, 700
HpyCH4V TGCA 9 cut(s) 109, 263, 537, 607, 683, 904, 994, 1025, 1179
HpyF10VI GCNNNNNNNGC 1 cut(s) 689
HpyF3I CTNAG 4 cut(s) 384, 789, 959, 1158
HpySE526I ACGT 2 cut(s) 340, 700
Hsp92I GRCGYC 1 cut(s) 1301
Hsp92II CATG 7 cut(s) 119, 311, 565, 812, 952, 998, 1112
HspAI GCGC 4 cut(s) 119, 181, 657, 690
Ksp22I TGATCA 1 cut(s) 508
Kzo9I GATC 5 cut(s) 40, 286, 508, 577, 1197
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 3 cut(s) 191, 230, 1087
Lsp1109I GCAGC 3 cut(s) 170, 359, 1144
LweI GCATC 2 cut(s) 586, 954
MaeI CTAG 1 cut(s) 551
MaeII ACGT 2 cut(s) 340, 700
MaeIII GTNAC 5 cut(s) 152, 488, 565, 1057, 1261
MalI GATC 5 cut(s) 42, 288, 510, 579, 1199
MboI GATC 5 cut(s) 40, 286, 508, 577, 1197
MboII GAAGA 8 cut(s) 167, 365, 380, 495, 742, 1157, 1215, 1355
MfeI CAATTG 2 cut(s) 522, 591
MflI RGATCY 2 cut(s) 286, 577
MhlI GDGCHC 1 cut(s) 609
MluCI AATT 3 cut(s) 318, 522, 591
MlyI GAGTC 2 cut(s) 562, 1087
MmeI TCCRAC 2 cut(s) 119, 347
MnlI CCTC 8 cut(s) 93, 199, 202, 379, 706, 880, 1021, 1051
MroXI GAANNNNTTC 2 cut(s) 318, 1207
MseI TTAA 7 cut(s) 147, 300, 477, 573, 672, 717, 1017
MslI CAYNNNNRTG 1 cut(s) 1004
MspCI CTTAAG 2 cut(s) 572, 671
MspI CCGG 2 cut(s) 194, 872
MspR9I CCNGG 2 cut(s) 237, 394
MunI CAATTG 2 cut(s) 522, 591
MvaI CCWGG 2 cut(s) 237, 394
MvnI CGCG 1 cut(s) 1190
MwoI GCNNNNNNNGC 1 cut(s) 689
NdeII GATC 5 cut(s) 40, 286, 508, 577, 1197
NlaIII CATG 7 cut(s) 119, 311, 565, 812, 952, 998, 1112
NlaIV GGNNCC 4 cut(s) 288, 390, 579, 1250
NmeAIII GCCGAG 1 cut(s) 24
NmuCI GTSAC 4 cut(s) 488, 565, 1057, 1261
NsbI TGCGCA 2 cut(s) 120, 691
NspI RCATGY 1 cut(s) 119
NspV TTCGAA 1 cut(s) 418
PaeI GCATGC 1 cut(s) 119
PagI TCATGA 1 cut(s) 948
PasI CCCWGGG 1 cut(s) 393
PciSI GCTCTTC 1 cut(s) 348
PdmI GAANNNNTTC 2 cut(s) 318, 1207
PfeI GAWTC 9 cut(s) 50, 130, 232, 785, 805, 842, 862, 1066, 1112
PflFI GACNNNGTC 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 235
PkrI GCNGC 3 cut(s) 185, 349, 1134
PleI GAGTC 2 cut(s) 562, 1087
PpsI GAGTC 2 cut(s) 562, 1087
Ppu21I YACGTR 1 cut(s) 341
PpuMI RGGWCCY 2 cut(s) 239, 388
PshBI ATTAAT 1 cut(s) 1017
Psp5II RGGWCCY 2 cut(s) 239, 388
Psp6I CCWGG 2 cut(s) 235, 392
PspGI CCWGG 2 cut(s) 235, 392
PspN4I GGNNCC 4 cut(s) 288, 390, 579, 1250
PspPI GGNCC 2 cut(s) 239, 388
PspPPI RGGWCCY 2 cut(s) 239, 388
PstI CTGCAG 1 cut(s) 1027
PstNI CAGNNNCTG 2 cut(s) 242, 1138
PsuI RGATCY 2 cut(s) 286, 577
PsyI GACNNNGTC 1 cut(s) 100
RsaI GTAC 1 cut(s) 1142
RsaNI GTAC 1 cut(s) 1141
RseI CAYNNNNRTG 1 cut(s) 1004
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 7 cut(s) 147, 300, 477, 573, 672, 717, 1017
SatI GCNGC 3 cut(s) 184, 348, 1133
Sau3AI GATC 5 cut(s) 40, 286, 508, 577, 1197
Sau96I GGNCC 2 cut(s) 239, 388
SchI GAGTC 2 cut(s) 562, 1087
ScrFI CCNGG 2 cut(s) 237, 394
SduI GDGCHC 1 cut(s) 609
SfaNI GCATC 2 cut(s) 586, 954
SfcI CTRYAG 2 cut(s) 453, 1023
SfuI TTCGAA 1 cut(s) 418
SinI GGWCC 2 cut(s) 239, 388
SmiMI CAYNNNNRTG 1 cut(s) 1004
SmlI CTYRAG 5 cut(s) 206, 327, 572, 671, 939
SmoI CTYRAG 5 cut(s) 206, 327, 572, 671, 939
SnaBI TACGTA 1 cut(s) 341
SphI GCATGC 1 cut(s) 119
Sse9I AATT 3 cut(s) 318, 522, 591
SsiI CCGC 4 cut(s) 73, 113, 140, 1188
SspI AATATT 1 cut(s) 893
SspMI CTAG 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 235, 392
StyI CCWWGG 1 cut(s) 741
TaaI ACNGT 5 cut(s) 167, 406, 494, 1073, 1140
TaiI ACGT 2 cut(s) 343, 703
TaqI TCGA 3 cut(s) 418, 1270, 1295
TasI AATT 3 cut(s) 318, 522, 591
TfiI GAWTC 9 cut(s) 50, 130, 232, 785, 805, 842, 862, 1066, 1112
Tru1I TTAA 7 cut(s) 147, 300, 477, 573, 672, 717, 1017
Tru9I TTAA 7 cut(s) 147, 300, 477, 573, 672, 717, 1017
TscAI CASTG 8 cut(s) 471, 589, 607, 666, 732, 988, 996, 1076
TseFI GTSAC 4 cut(s) 488, 565, 1057, 1261
TseI GCWGC 3 cut(s) 183, 347, 1132
Tsp45I GTSAC 4 cut(s) 488, 565, 1057, 1261
TspDTI ATGAA 6 cut(s) 17, 293, 693, 894, 965, 1272
TspGWI ACGGA 2 cut(s) 257, 1343
TspRI CASTG 8 cut(s) 471, 589, 607, 666, 732, 988, 996, 1076
Tth111I GACNNNGTC 1 cut(s) 100
Vha464I CTTAAG 2 cut(s) 572, 671
VneI GTGCAC 1 cut(s) 605
VpaK11BI GGWCC 2 cut(s) 239, 388
VspI ATTAAT 1 cut(s) 1017
XceI RCATGY 1 cut(s) 119
XmiI GTMKAC 1 cut(s) 21
XmnI GAANNNNTTC 2 cut(s) 318, 1207
XspI CTAG 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.