Rroxscaffold_3G00274920

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006732
Physical Location & Seq
Forward (+)
66530823 .. 66539889
9067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274920.1

Sequence Viewer

Length: 1182 bp
ATGGATCAAGAATGTTGTAATTCTATTCATCTGAGTTATTACATGAAATTGCTAGATAATGTAATGGATCATAATTACTTGCTCAACAAGATTAAGTGGGAAGGAGTCCCATTTTATAGGAAAGGGAGGCTCATTCCTTCACTTTGTGGTTCACTAACAGATGCCATGCGGCGGCCAAGAAGACACGGAGGGCAGCTGGGCGTGGAGCGGCGATCACATGGAGGGCCAAGTCGTGGCCGAGGACGCCGTAGAGAGAGAAAAAGCCGGCCCTGGGAAGAAGCTCTCGACGTAGACGACGACAAGGCCGTGGCGGCTCTCGGACCCCTTTGGGTTCGATCAAGAAGGGGCTTGGGCACTCACAGATTAGCTAAGGTTGTTGCTATGATCAAATCTTGCACCCCAAATGGTCTTGGTGATCTCATGTTGACTTTGAAGGATCCTACAGGTACAATTGGTGCTACCATCCACCGCAAAGCCCTTTCTGAGGGAGAGTTTGGGAAGAGCATTTCTGTGGGTGCGGTTTTGGTTCTGCAGAAGGTTGCTGTGTTTTCCCCCTCACACTCTGCATGTTATCTTAATGTGACGGTGAGCAACATTGTCAAGGTCATTTCCAAGGATAGTGGGCCTCTTGCCACAAAAGATTTTCCAATTTTGTCAGTTAATACTCCTGCTCTCATCAGTGCAGAAAATAGTAAAATGCTACGGACGCCACAAGAAAAACTCCTCCTGTCACAGGAAAGTACTCAGCGAATCATGAAATGTCTGAGACAAAATTCCAAGGTGATTGGGAGTGAAAATATTGAGAAGCATATGGAAACTGGCCACGCAGCACCAGGAAGGATTTTCTCTGCTCATGTGCACTCTGGAAGCCAATTTGCTGCTGTAGAGATAGAGCCTTCATTGGTGGAAAAGGCTATGCCTAATGGAATCACTAAAATGTCTGCTAGAGAGGACATTATTGATACAGAACAAGTAACTGGGGTTGCTGAGGAGCTCAGAATGGCTGAAGAGGACAATTCATCAAGCATCATCCAACCCATAAGTGGTTCAGCAAACTCGACTGAGATTCCTGATAATCATGGGAGAATAACAGGAGCAAAAAGGCCAAGGGAGCCACTGAGCTTAACAACTGCGCTCCCAGACTATACAGAAGAACAACTAAATCTGTTTGACTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

393

Amino Acids

43.25

Weight (kDa)

8.93

Isoelectric Point (pI)

56.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HROB PF15072 121 - 206 1.1e-28 Homologous recombination OB-fold protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 233
AccBSI CCGCTC 1 cut(s) 208
AccI GTMKAC 1 cut(s) 291
AciI CCGC 6 cut(s) 169, 172, 208, 311, 469, 518
AclWI GGATC 4 cut(s) 12, 75, 431, 444
AcoI YGGCCR 3 cut(s) 173, 235, 820
AcsI RAATTY 1 cut(s) 772
AcuI CTGAAG 1 cut(s) 1026
AcyI GRCGYC 2 cut(s) 244, 707
AdeI CACNNNGTG 1 cut(s) 146
AfaI GTAC 2 cut(s) 448, 742
AfiI CCNNNNNNNGG 6 cut(s) 171, 233, 271, 484, 733, 1043
AgsI TTSAA 1 cut(s) 433
AjnI CCWGG 2 cut(s) 269, 832
AluBI AGCT 5 cut(s) 196, 281, 368, 994, 1122
AluI AGCT 5 cut(s) 196, 281, 368, 994, 1122
Alw21I GWGCWC 2 cut(s) 861, 996
Alw26I GTCTC 1 cut(s) 760
Alw44I GTGCAC 1 cut(s) 857
AlwI GGATC 4 cut(s) 12, 75, 431, 444
AoxI GGCC 8 cut(s) 173, 224, 235, 266, 303, 623, 820, 1103
ApaLI GTGCAC 1 cut(s) 857
ApeKI GCWGC 3 cut(s) 193, 827, 878
ApoI RAATTY 1 cut(s) 772
AspLEI GCGC 1 cut(s) 1135
AspS9I GGNCC 4 cut(s) 224, 267, 320, 623
AsuHPI GGTGA 3 cut(s) 425, 598, 793
AvaII GGWCC 1 cut(s) 320
BaeGI GKGCMC 2 cut(s) 356, 861
BalI TGGCCA 1 cut(s) 822
BamHI GGATCC 1 cut(s) 436
BanII GRGCYC 1 cut(s) 996
BbsI GAAGAC 1 cut(s) 187
Bbv12I GWGCWC 2 cut(s) 861, 996
BbvCI CCTCAGC 1 cut(s) 987
BbvI GCAGC 3 cut(s) 205, 839, 865
BccI CCATC 1 cut(s) 470
BceAI ACGGC 2 cut(s) 231, 290
BciT130I CCWGG 2 cut(s) 271, 834
BclI TGATCA 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 760
BfaI CTAG 2 cut(s) 53, 945
BfmI CTRYAG 3 cut(s) 441, 530, 882
BglI GCCNNNNNGGC 1 cut(s) 311
BisI GCNGC 7 cut(s) 170, 173, 194, 209, 312, 828, 879
BlsI GCNGC 7 cut(s) 171, 174, 195, 210, 313, 829, 880
BmcAI AGTACT 1 cut(s) 742
Bme1390I CCNGG 2 cut(s) 271, 834
Bme18I GGWCC 1 cut(s) 320
BmgT120I GGNCC 4 cut(s) 224, 267, 320, 623
BmiI GGNNCC 3 cut(s) 322, 438, 1113
BmrFI CCNGG 2 cut(s) 271, 834
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 2 cut(s) 151, 1035
BmuI ACTGGG 1 cut(s) 987
BpiI GAAGAC 1 cut(s) 187
Bpu10I CCTNAGC 2 cut(s) 369, 987
BsaHI GRCGYC 2 cut(s) 244, 707
BsaJI CCNNGG 7 cut(s) 238, 269, 270, 306, 612, 777, 1106
Bsc4I CCNNNNNNNGG 6 cut(s) 171, 233, 271, 484, 733, 1043
Bse118I RCCGGY 1 cut(s) 264
Bse1I ACTGG 2 cut(s) 823, 982
BseBI CCWGG 2 cut(s) 271, 834
BseDI CCNNGG 7 cut(s) 238, 269, 270, 306, 612, 777, 1106
BseGI GGATG 2 cut(s) 462, 1029
BseLI CCNNNNNNNGG 6 cut(s) 171, 233, 271, 484, 733, 1043
BseMII CTCAG 8 cut(s) 23, 474, 755, 758, 978, 1009, 1053, 1109
BseNI ACTGG 2 cut(s) 823, 982
BseRI GAGGAG 2 cut(s) 713, 1004
BseSI GKGCMC 2 cut(s) 356, 861
BseXI GCAGC 3 cut(s) 205, 839, 865
BseYI CCCAGC 1 cut(s) 196
BsgI GTGCAG 1 cut(s) 702
BshFI GGCC 8 cut(s) 175, 226, 237, 268, 305, 625, 822, 1105
BsiHKAI GWGCWC 2 cut(s) 861, 996
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 6 cut(s) 171, 233, 271, 484, 733, 1043
BsmAI GTCTC 1 cut(s) 760
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 8 cut(s) 175, 226, 237, 268, 305, 625, 822, 1105
Bsp1286I GDGCHC 3 cut(s) 356, 861, 996
Bsp143I GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
BspACI CCGC 6 cut(s) 169, 172, 208, 311, 469, 518
BspANI GGCC 8 cut(s) 175, 226, 237, 268, 305, 625, 822, 1105
BspCNI CTCAG 8 cut(s) 24, 475, 756, 757, 979, 1008, 1054, 1110
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 3 cut(s) 322, 438, 1113
BspMAI CTGCAG 1 cut(s) 534
BspPI GGATC 4 cut(s) 12, 75, 431, 444
BspQI GCTCTTC 1 cut(s) 494
BsrBI CCGCTC 1 cut(s) 208
BsrFI RCCGGY 1 cut(s) 264
BsrI ACTGG 2 cut(s) 823, 982
BssAI RCCGGY 1 cut(s) 264
BssECI CCNNGG 7 cut(s) 238, 269, 270, 306, 612, 777, 1106
BssMI GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
BssNI GRCGYC 2 cut(s) 244, 707
BssT1I CCWWGG 3 cut(s) 612, 777, 1106
Bst2UI CCWGG 2 cut(s) 271, 834
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 2 cut(s) 494, 1002
BstACI GRCGYC 2 cut(s) 244, 707
BstC8I GCNNGC 1 cut(s) 266
BstDEI CTNAG 9 cut(s) 32, 369, 483, 744, 764, 987, 995, 1062, 1118
BstDSI CCRYGG 1 cut(s) 306
BstENI CCTNNNNNAGG 2 cut(s) 482, 731
BstF5I GGATG 2 cut(s) 462, 1029
BstHHI GCGC 1 cut(s) 1135
BstKTI GATC 7 cut(s) 7, 70, 215, 338, 387, 418, 439
BstMAI GTCTC 1 cut(s) 760
BstMBI GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
BstMWI GCNNNNNNNGC 4 cut(s) 243, 311, 706, 1111
BstNI CCWGG 2 cut(s) 271, 834
BstNSI RCATGY 1 cut(s) 570
BstSCI CCNGG 2 cut(s) 269, 832
BstSFI CTRYAG 3 cut(s) 441, 530, 882
BstSLI GKGCMC 2 cut(s) 356, 861
BstV1I GCAGC 3 cut(s) 205, 839, 865
BstV2I GAAGAC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 8 cut(s) 175, 226, 237, 268, 305, 625, 822, 1105
BtgI CCRYGG 1 cut(s) 306
BtsCI GGATG 2 cut(s) 462, 1029
BtsIMutI CAGTG 2 cut(s) 685, 1115
Cac8I GCNNGC 1 cut(s) 266
CciI TCATGA 1 cut(s) 753
CfoI GCGC 1 cut(s) 1135
Cfr10I RCCGGY 1 cut(s) 264
Cfr13I GGNCC 4 cut(s) 224, 267, 320, 623
CseI GACGC 2 cut(s) 252, 715
Csp6I GTAC 2 cut(s) 447, 741
CspCI CAANNNNNGTGG 2 cut(s) 601, 636
CviAII CATG 8 cut(s) 43, 166, 218, 421, 567, 754, 854, 1079
CviQI GTAC 2 cut(s) 447, 741
DdeI CTNAG 9 cut(s) 32, 369, 483, 744, 764, 987, 995, 1062, 1118
DpnI GATC 7 cut(s) 6, 69, 214, 337, 386, 417, 438
DpnII GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
DraIII CACNNNGTG 1 cut(s) 146
EaeI YGGCCR 3 cut(s) 173, 235, 820
Eam1104I CTCTTC 2 cut(s) 494, 1002
EarI CTCTTC 2 cut(s) 494, 1002
Ecl136II GAGCTC 1 cut(s) 994
Eco130I CCWWGG 3 cut(s) 612, 777, 1106
Eco24I GRGCYC 1 cut(s) 996
Eco47I GGWCC 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 1026
EcoICRI GAGCTC 1 cut(s) 994
EcoNI CCTNNNNNAGG 2 cut(s) 482, 731
EcoRII CCWGG 2 cut(s) 269, 832
EcoT14I CCWWGG 3 cut(s) 612, 777, 1106
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 3 cut(s) 612, 777, 1106
FaeI CATG 8 cut(s) 46, 169, 221, 424, 570, 757, 857, 1082
FaqI GGGAC 1 cut(s) 92
FatI CATG 8 cut(s) 42, 165, 217, 420, 566, 753, 853, 1078
FauNDI CATATG 1 cut(s) 810
FbaI TGATCA 1 cut(s) 384
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 7 cut(s) 170, 173, 194, 209, 312, 828, 879
FokI GGATG 2 cut(s) 449, 1016
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 7 cut(s) 170, 173, 194, 209, 312, 828, 879
FspBI CTAG 2 cut(s) 53, 945
GlaI GCGC 1 cut(s) 1134
GluI GCNGC 7 cut(s) 170, 173, 194, 209, 312, 828, 879
GsaI CCCAGC 1 cut(s) 200
HaeIII GGCC 8 cut(s) 175, 226, 237, 268, 305, 625, 822, 1105
HapII CCGG 1 cut(s) 265
HgaI GACGC 2 cut(s) 252, 715
HhaI GCGC 1 cut(s) 1135
Hin1I GRCGYC 2 cut(s) 244, 707
Hin1II CATG 8 cut(s) 46, 169, 221, 424, 570, 757, 857, 1082
Hin6I GCGC 1 cut(s) 1133
HinP1I GCGC 1 cut(s) 1133
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 4 cut(s) 105, 750, 927, 1066
HpaII CCGG 1 cut(s) 265
HphI GGTGA 3 cut(s) 425, 598, 793
Hpy166II GTNNAC 4 cut(s) 152, 292, 426, 859
Hpy188I TCNGA 5 cut(s) 33, 320, 484, 765, 998
Hpy188III TCNNGA 6 cut(s) 8, 284, 339, 754, 864, 1070
Hpy8I GTNNAC 4 cut(s) 152, 292, 426, 859
Hpy99I CGWCG 2 cut(s) 290, 299
HpyAV CCTTC 7 cut(s) 95, 147, 336, 427, 529, 831, 906
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 5 cut(s) 396, 532, 566, 683, 859
HpyF10VI GCNNNNNNNGC 4 cut(s) 243, 311, 706, 1111
HpyF3I CTNAG 9 cut(s) 32, 369, 483, 744, 764, 987, 995, 1062, 1118
HpySE526I ACGT 1 cut(s) 288
Hsp92I GRCGYC 2 cut(s) 244, 707
Hsp92II CATG 8 cut(s) 46, 169, 221, 424, 570, 757, 857, 1082
HspAI GCGC 1 cut(s) 1133
KroI GCCGGC 1 cut(s) 264
KroNI GCCGGC 1 cut(s) 266
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 5 cut(s) 205, 991, 1094, 1111, 1140
Lsp1109I GCAGC 3 cut(s) 205, 839, 865
LweI GCATC 2 cut(s) 151, 1035
MaeI CTAG 2 cut(s) 53, 945
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 580, 729, 973
MalI GATC 7 cut(s) 6, 69, 214, 337, 386, 417, 438
MbiI CCGCTC 1 cut(s) 208
MboI GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
MboII GAAGA 5 cut(s) 192, 287, 511, 1019, 1163
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 356, 861, 996
MlsI TGGCCA 1 cut(s) 822
MluCI AATT 8 cut(s) 19, 47, 73, 450, 648, 772, 872, 1015
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 1 cut(s) 114
MmeI TCCRAC 1 cut(s) 1057
Mox20I TGGCCA 1 cut(s) 822
MroNI GCCGGC 1 cut(s) 264
MscI TGGCCA 1 cut(s) 822
MseI TTAA 4 cut(s) 93, 576, 660, 1124
MslI CAYNNNNRTG 2 cut(s) 509, 935
Msp20I TGGCCA 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 196
MspI CCGG 1 cut(s) 265
MspR9I CCNGG 2 cut(s) 271, 834
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 2 cut(s) 271, 834
MwoI GCNNNNNNNGC 4 cut(s) 243, 311, 706, 1111
NaeI GCCGGC 1 cut(s) 266
NdeI CATATG 1 cut(s) 810
NdeII GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
NgoMIV GCCGGC 1 cut(s) 264
NlaIII CATG 8 cut(s) 46, 169, 221, 424, 570, 757, 857, 1082
NlaIV GGNNCC 3 cut(s) 322, 438, 1113
NmeAIII GCCGAG 1 cut(s) 263
NmuCI GTSAC 2 cut(s) 580, 729
NspI RCATGY 1 cut(s) 570
PagI TCATGA 1 cut(s) 753
PasI CCCWGGG 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 494
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 294, 303
PdiI GCCGGC 1 cut(s) 266
PfeI GAWTC 3 cut(s) 750, 927, 1066
PflMI CCANNNNNTGG 1 cut(s) 233
PkrI GCNGC 7 cut(s) 171, 174, 195, 210, 313, 829, 880
PleI GAGTC 1 cut(s) 113
PpsI GAGTC 1 cut(s) 113
Psp124BI GAGCTC 1 cut(s) 996
Psp6I CCWGG 2 cut(s) 269, 832
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 2 cut(s) 269, 832
PspN4I GGNNCC 3 cut(s) 322, 438, 1113
PspPI GGNCC 4 cut(s) 224, 267, 320, 623
PstI CTGCAG 1 cut(s) 534
PsuI RGATCY 1 cut(s) 436
PvuII CAGCTG 1 cut(s) 196
RsaI GTAC 2 cut(s) 448, 742
RsaNI GTAC 2 cut(s) 447, 741
RseI CAYNNNNRTG 2 cut(s) 509, 935
SacI GAGCTC 1 cut(s) 996
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 4 cut(s) 93, 576, 660, 1124
SatI GCNGC 7 cut(s) 170, 173, 194, 209, 312, 828, 879
Sau3AI GATC 7 cut(s) 4, 67, 212, 335, 384, 415, 436
Sau96I GGNCC 4 cut(s) 224, 267, 320, 623
ScaI AGTACT 1 cut(s) 742
SchI GAGTC 1 cut(s) 114
ScrFI CCNGG 2 cut(s) 271, 834
SduI GDGCHC 3 cut(s) 356, 861, 996
SfaNI GCATC 2 cut(s) 151, 1035
SfcI CTRYAG 3 cut(s) 441, 530, 882
SinI GGWCC 1 cut(s) 320
SmiMI CAYNNNNRTG 2 cut(s) 509, 935
Sse9I AATT 8 cut(s) 19, 47, 73, 450, 648, 772, 872, 1015
SsiI CCGC 6 cut(s) 169, 172, 208, 311, 469, 518
SspI AATATT 1 cut(s) 799
SspMI CTAG 2 cut(s) 53, 945
SstI GAGCTC 1 cut(s) 996
StyD4I CCNGG 2 cut(s) 269, 832
StyI CCWWGG 3 cut(s) 612, 777, 1106
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 291
TaqI TCGA 3 cut(s) 285, 334, 1058
TasI AATT 8 cut(s) 19, 47, 73, 450, 648, 772, 872, 1015
TatI WGTACW 1 cut(s) 740
TauI GCSGC 4 cut(s) 172, 175, 211, 314
TfiI GAWTC 3 cut(s) 750, 927, 1066
Tru1I TTAA 4 cut(s) 93, 576, 660, 1124
Tru9I TTAA 4 cut(s) 93, 576, 660, 1124
TscAI CASTG 2 cut(s) 685, 1122
TseFI GTSAC 2 cut(s) 580, 729
TseI GCWGC 3 cut(s) 193, 827, 878
Tsp45I GTSAC 2 cut(s) 580, 729
TspDTI ATGAA 5 cut(s) 17, 59, 770, 888, 1008
TspGWI ACGGA 2 cut(s) 201, 718
TspRI CASTG 2 cut(s) 685, 1122
Van91I CCANNNNNTGG 1 cut(s) 233
VneI GTGCAC 1 cut(s) 857
VpaK11BI GGWCC 1 cut(s) 320
XagI CCTNNNNNAGG 2 cut(s) 482, 731
XapI RAATTY 1 cut(s) 772
XceI RCATGY 1 cut(s) 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 2 cut(s) 53, 945
ZrmI AGTACT 1 cut(s) 742
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.