Rw0G010170

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00423
OG0006732
Physical Location & Seq
Forward (+)
13860 .. 16224
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G010170.1

Sequence Viewer

Length: 1188 bp
ATGGAACCCTGGGAAGAAGCTCTCGACGTAGACGACGACGTTTCGGAGCTCCCCTCTTTCTCTCTTCGCCCTTGCAACCACTCTTCCTCTCCTTCCCTAAACCCCCAACCCAAACCCTCCTCTCCATCTCCGGCCCTCATTCCGGGCCCCGCCGGCGCCGTTCAGGCCGCGATGCACCGCCGCTCCCAGACCTCCTCCTCCGGCGAGCCGATTCCGACCACGCAGGAGGTCATAAGGAGAGTCTTCGAGAACGGCGACGAGGAAGACGACGATTTCGCCGCCGATTCTTGGCTCCGCGCTCTCGATTCAGAAGGCGGTGGCGGCCCTCGGACCCTTTTGGGCTCGATCAAGAAGGGGCTCGGCAGTCACAGAGTAGCTAAGGTTGTTGCTATGATCAAATCTTGCACCCCAAATGGTCTTGGTGATCTCATGTTGACTTTGAAGGATCCTACAGGTACAATTGGTGCTACCATCCACCGCAAAGCCCTTTCTGAGGGAGAGTTTGGCAAGAGCGTTTCTGTGGGTGCGGTTTTGGTTCTGCAGAAGGTTGCTGTGTTTTCCCCCTCACACTCTGCATGTTATCTTAATGTGACGGTGAGCAACATTGTCAAGGTCATTTCCAAGGATAGTGGGCCTCTTGCCACAAAAGATTTTCCAATTTCGTCAGTTAATACTCCTGCTCTCATCTGTGAAAATAGTAAAATGTTACGGATGCCACAAGAAAAACTCCTCCCATCACAGGATAGTTCTCAGCGAATCATGAAATGTCTGAGACAAAATTCCAAGGTGATTGGGAGTGAAAATATTGAGAAGCATATGGAAACTGGCCACGTGGCACCAGGAGGGATTTTCTCTGCTCATGTGCACTCTGGAAGCCAATTTGCTGCTGTAGAGATAGAGCCTTCATTGGTGGAAAAGGCTATGCCTAATGGAATCACTAAAATGTCTGTTAGAGAGGACATTATTGATACAGAACAAGTAACTGGGGTTGCTGAGGAGCTCAGAATGGCTGAAGAGGACAATTCATCAAGCATCATCCAACCCCTTAGTGGTTCAGCAAACTCGACTGAGATTCTTGATAATCATGGGAGAATAACAGGAGCAAAAAGGCCAAGGCAGCCACTGAGCTTAACAACTGCGCTCCCAGACTACACAGAAGAACAACTAAATCTGTTTGACTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

395

Amino Acids

42.3

Weight (kDa)

5.24

Isoelectric Point (pI)

62.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HROB PF15072 124 - 209 7.5e-29 Homologous recombination OB-fold protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 155, 835
AccBSI CCGCTC 1 cut(s) 183
AccI GTMKAC 1 cut(s) 30
AccII CGCG 2 cut(s) 170, 297
AclWI GGATC 2 cut(s) 440, 453
AcoI YGGCCR 1 cut(s) 826
AcsI RAATTY 1 cut(s) 778
AcuI CTGAAG 1 cut(s) 1032
AcvI CACGTG 1 cut(s) 832
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 1 cut(s) 457
AfiI CCNNNNNNNGG 5 cut(s) 142, 288, 493, 739, 1049
AgsI TTSAA 1 cut(s) 442
AjnI CCWGG 2 cut(s) 8, 838
AluBI AGCT 5 cut(s) 20, 49, 377, 1000, 1128
AluI AGCT 5 cut(s) 20, 49, 377, 1000, 1128
Alw21I GWGCWC 3 cut(s) 51, 867, 1002
Alw26I GTCTC 1 cut(s) 766
Alw44I GTGCAC 1 cut(s) 863
AlwI GGATC 2 cut(s) 440, 453
AlwNI CAGNNNCTG 1 cut(s) 1123
AoxI GGCC 7 cut(s) 132, 145, 165, 322, 632, 826, 1109
ApaI GGGCCC 1 cut(s) 149
ApaLI GTGCAC 1 cut(s) 863
ApeKI GCWGC 2 cut(s) 884, 1117
ApoI RAATTY 1 cut(s) 778
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspLEI GCGC 3 cut(s) 158, 299, 1141
AspS9I GGNCC 6 cut(s) 133, 145, 146, 323, 330, 632
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 3 cut(s) 434, 607, 799
AvaII GGWCC 1 cut(s) 330
BaeGI GKGCMC 2 cut(s) 149, 867
BalI TGGCCA 1 cut(s) 828
BamHI GGATCC 1 cut(s) 445
BanI GGYRCC 2 cut(s) 155, 835
BanII GRGCYC 5 cut(s) 51, 149, 344, 360, 1002
BbrPI CACGTG 1 cut(s) 832
BbsI GAAGAC 2 cut(s) 235, 270
Bbv12I GWGCWC 3 cut(s) 51, 867, 1002
BbvCI CCTCAGC 1 cut(s) 993
BbvI GCAGC 2 cut(s) 871, 1129
BccI CCATC 3 cut(s) 133, 479, 742
BceAI ACGGC 2 cut(s) 143, 268
BciT130I CCWGG 2 cut(s) 10, 840
BclI TGATCA 1 cut(s) 393
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 766
BfmI CTRYAG 3 cut(s) 450, 539, 888
BfoI RGCGCY 1 cut(s) 159
BglI GCCNNNNNGGC 2 cut(s) 153, 164
BisI GCNGC 6 cut(s) 168, 181, 279, 322, 885, 1118
BlsI GCNGC 6 cut(s) 169, 182, 280, 323, 886, 1119
Bme1390I CCNGG 3 cut(s) 10, 144, 840
Bme18I GGWCC 1 cut(s) 330
BmgT120I GGNCC 6 cut(s) 133, 145, 146, 323, 330, 632
BmiI GGNNCC 8 cut(s) 6, 147, 148, 157, 293, 332, 447, 837
BmrFI CCNGG 3 cut(s) 10, 144, 840
BmrI ACTGGG 1 cut(s) 993
BmsI GCATC 3 cut(s) 162, 702, 1041
BmuI ACTGGG 1 cut(s) 993
BpiI GAAGAC 2 cut(s) 235, 270
BplI GAGNNNNNCTC 2 cut(s) 38, 70
Bpu10I CCTNAGC 2 cut(s) 378, 993
BpuMI CCSGG 1 cut(s) 144
BsaAI YACGTR 1 cut(s) 832
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 6 cut(s) 8, 9, 326, 621, 783, 1112
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 5 cut(s) 142, 288, 493, 739, 1049
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 829, 988
BseBI CCWGG 2 cut(s) 10, 840
BseDI CCNNGG 6 cut(s) 8, 9, 326, 621, 783, 1112
BseGI GGATG 3 cut(s) 471, 717, 1035
BseLI CCNNNNNNNGG 5 cut(s) 142, 288, 493, 739, 1049
BseMII CTCAG 7 cut(s) 483, 761, 764, 984, 1015, 1059, 1115
BseNI ACTGG 2 cut(s) 829, 988
BseRI GAGGAG 5 cut(s) 109, 184, 187, 719, 1010
BseSI GKGCMC 2 cut(s) 149, 867
BseXI GCAGC 2 cut(s) 871, 1129
Bsh1236I CGCG 2 cut(s) 170, 297
BshFI GGCC 7 cut(s) 134, 147, 167, 324, 634, 828, 1111
BshNI GGYRCC 2 cut(s) 155, 835
BsiHKAI GWGCWC 3 cut(s) 51, 867, 1002
BsiSI CCGG 4 cut(s) 131, 143, 153, 201
BslI CCNNNNNNNGG 5 cut(s) 142, 288, 493, 739, 1049
BsmAI GTCTC 1 cut(s) 766
BsnI GGCC 7 cut(s) 134, 147, 167, 324, 634, 828, 1111
Bsp120I GGGCCC 1 cut(s) 145
Bsp1286I GDGCHC 6 cut(s) 51, 149, 344, 360, 867, 1002
Bsp143I GATC 4 cut(s) 345, 393, 424, 445
BspANI GGCC 7 cut(s) 134, 147, 167, 324, 634, 828, 1111
BspCNI CTCAG 7 cut(s) 484, 762, 763, 985, 1014, 1060, 1116
BspFNI CGCG 2 cut(s) 170, 297
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 8 cut(s) 6, 147, 148, 157, 293, 332, 447, 837
BspMAI CTGCAG 1 cut(s) 543
BspPI GGATC 2 cut(s) 440, 453
BspT107I GGYRCC 2 cut(s) 155, 835
BsrBI CCGCTC 1 cut(s) 183
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 829, 988
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 6 cut(s) 8, 9, 326, 621, 783, 1112
BssMI GATC 4 cut(s) 345, 393, 424, 445
BssNI GRCGYC 1 cut(s) 156
BssT1I CCWWGG 3 cut(s) 621, 783, 1112
Bst2UI CCWGG 2 cut(s) 10, 840
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 3 cut(s) 69, 88, 1008
BstACI GRCGYC 1 cut(s) 156
BstBAI YACGTR 1 cut(s) 832
BstC8I GCNNGC 2 cut(s) 154, 206
BstDEI CTNAG 9 cut(s) 378, 492, 750, 770, 993, 1001, 1046, 1068, 1124
BstENI CCTNNNNNAGG 1 cut(s) 491
BstF5I GGATG 3 cut(s) 471, 717, 1035
BstFNI CGCG 2 cut(s) 170, 297
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 3 cut(s) 158, 299, 1141
BstKTI GATC 4 cut(s) 348, 396, 427, 448
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 4 cut(s) 345, 393, 424, 445
BstMWI GCNNNNNNNGC 4 cut(s) 153, 164, 321, 1117
BstNI CCWGG 2 cut(s) 10, 840
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 3 cut(s) 8, 142, 838
BstSFI CTRYAG 3 cut(s) 450, 539, 888
BstSLI GKGCMC 2 cut(s) 149, 867
BstUI CGCG 2 cut(s) 170, 297
BstV1I GCAGC 2 cut(s) 871, 1129
BstV2I GAAGAC 2 cut(s) 235, 270
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BsuRI GGCC 7 cut(s) 134, 147, 167, 324, 634, 828, 1111
BtgZI GCGATG 1 cut(s) 185
BtsCI GGATG 3 cut(s) 471, 717, 1035
BtsIMutI CAGTG 1 cut(s) 1121
Cac8I GCNNGC 2 cut(s) 154, 206
CaiI CAGNNNCTG 1 cut(s) 1123
CciI TCATGA 1 cut(s) 759
CfoI GCGC 3 cut(s) 158, 299, 1141
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 6 cut(s) 133, 145, 146, 323, 330, 632
Csp6I GTAC 1 cut(s) 456
CspCI CAANNNNNGTGG 2 cut(s) 610, 645
CviAII CATG 5 cut(s) 430, 576, 760, 860, 1085
CviQI GTAC 1 cut(s) 456
DdeI CTNAG 9 cut(s) 378, 492, 750, 770, 993, 1001, 1046, 1068, 1124
DinI GGCGCC 1 cut(s) 157
DpnI GATC 4 cut(s) 347, 395, 426, 447
DpnII GATC 4 cut(s) 345, 393, 424, 445
EaeI YGGCCR 1 cut(s) 826
Eam1104I CTCTTC 3 cut(s) 69, 88, 1008
EarI CTCTTC 3 cut(s) 69, 88, 1008
Ecl136II GAGCTC 2 cut(s) 49, 1000
Eco130I CCWWGG 3 cut(s) 621, 783, 1112
Eco24I GRGCYC 5 cut(s) 51, 149, 344, 360, 1002
Eco47I GGWCC 1 cut(s) 330
Eco53kI GAGCTC 2 cut(s) 49, 1000
Eco57I CTGAAG 1 cut(s) 1032
Eco72I CACGTG 1 cut(s) 832
EcoICRI GAGCTC 2 cut(s) 49, 1000
EcoNI CCTNNNNNAGG 1 cut(s) 491
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 8, 838
EcoT14I CCWWGG 3 cut(s) 621, 783, 1112
EcoT38I GRGCYC 5 cut(s) 51, 149, 344, 360, 1002
EgeI GGCGCC 1 cut(s) 157
EheI GGCGCC 1 cut(s) 157
ErhI CCWWGG 3 cut(s) 621, 783, 1112
FaeI CATG 5 cut(s) 433, 579, 763, 863, 1088
FatI CATG 5 cut(s) 429, 575, 759, 859, 1084
FauI CCCGC 1 cut(s) 157
FauNDI CATATG 1 cut(s) 816
FbaI TGATCA 1 cut(s) 393
FblI GTMKAC 1 cut(s) 30
Fnu4HI GCNGC 6 cut(s) 168, 181, 279, 322, 885, 1118
FokI GGATG 3 cut(s) 458, 724, 1022
FriOI GRGCYC 5 cut(s) 51, 149, 344, 360, 1002
Fsp4HI GCNGC 6 cut(s) 168, 181, 279, 322, 885, 1118
GlaI GCGC 3 cut(s) 157, 298, 1140
GluI GCNGC 6 cut(s) 168, 181, 279, 322, 885, 1118
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 7 cut(s) 134, 147, 167, 324, 634, 828, 1111
HapII CCGG 4 cut(s) 131, 143, 153, 201
HhaI GCGC 3 cut(s) 158, 299, 1141
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 5 cut(s) 433, 579, 763, 863, 1088
Hin6I GCGC 3 cut(s) 156, 297, 1139
HinP1I GCGC 3 cut(s) 156, 297, 1139
HincII GTYRAC 1 cut(s) 435
HindII GTYRAC 1 cut(s) 435
HinfI GANTC 7 cut(s) 211, 240, 284, 305, 756, 933, 1072
HpaII CCGG 4 cut(s) 131, 143, 153, 201
HphI GGTGA 3 cut(s) 434, 607, 799
Hpy166II GTNNAC 3 cut(s) 31, 435, 865
Hpy188I TCNGA 7 cut(s) 46, 216, 310, 330, 493, 771, 1004
Hpy188III TCNNGA 7 cut(s) 23, 247, 302, 349, 760, 870, 1076
Hpy8I GTNNAC 3 cut(s) 31, 435, 865
Hpy99I CGWCG 5 cut(s) 29, 38, 41, 260, 272
HpyAV CCTTC 6 cut(s) 102, 305, 346, 436, 538, 912
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4IV ACGT 3 cut(s) 27, 39, 831
HpyCH4V TGCA 6 cut(s) 75, 175, 405, 541, 575, 865
HpyF10VI GCNNNNNNNGC 4 cut(s) 153, 164, 321, 1117
HpyF3I CTNAG 9 cut(s) 378, 492, 750, 770, 993, 1001, 1046, 1068, 1124
HpySE526I ACGT 3 cut(s) 27, 39, 831
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 5 cut(s) 433, 579, 763, 863, 1088
HspAI GCGC 3 cut(s) 156, 297, 1139
KasI GGCGCC 1 cut(s) 155
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 393
Kzo9I GATC 4 cut(s) 345, 393, 424, 445
LmnI GCTCC 7 cut(s) 46, 54, 188, 297, 997, 1100, 1146
Lsp1109I GCAGC 2 cut(s) 871, 1129
LweI GCATC 3 cut(s) 162, 702, 1041
MaeII ACGT 3 cut(s) 27, 39, 831
MaeIII GTNAC 4 cut(s) 365, 589, 705, 979
MalI GATC 4 cut(s) 347, 395, 426, 447
MbiI CCGCTC 1 cut(s) 183
MboI GATC 4 cut(s) 345, 393, 424, 445
MboII GAAGA 7 cut(s) 26, 56, 75, 235, 275, 1025, 1169
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 6 cut(s) 51, 149, 344, 360, 867, 1002
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 5 cut(s) 459, 657, 778, 878, 1021
MluNI TGGCCA 1 cut(s) 828
Mly113I GGCGCC 1 cut(s) 156
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 2 cut(s) 239, 1063
Mox20I TGGCCA 1 cut(s) 828
MreI CGCCGGCG 1 cut(s) 152
MroNI GCCGGC 1 cut(s) 152
MscI TGGCCA 1 cut(s) 828
MseI TTAA 3 cut(s) 585, 669, 1130
MslI CAYNNNNRTG 1 cut(s) 941
Msp20I TGGCCA 1 cut(s) 828
MspI CCGG 4 cut(s) 131, 143, 153, 201
MspR9I CCNGG 3 cut(s) 10, 144, 840
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 2 cut(s) 10, 840
MvnI CGCG 2 cut(s) 170, 297
MwoI GCNNNNNNNGC 4 cut(s) 153, 164, 321, 1117
NaeI GCCGGC 1 cut(s) 154
NarI GGCGCC 1 cut(s) 156
NciI CCSGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 816
NdeII GATC 4 cut(s) 345, 393, 424, 445
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 5 cut(s) 433, 579, 763, 863, 1088
NlaIV GGNNCC 8 cut(s) 6, 147, 148, 157, 293, 332, 447, 837
NmeAIII GCCGAG 1 cut(s) 339
NmuCI GTSAC 2 cut(s) 365, 589
NspI RCATGY 1 cut(s) 579
PagI TCATGA 1 cut(s) 759
PasI CCCWGGG 1 cut(s) 9
PcsI WCGNNNNNNNCGW 4 cut(s) 30, 33, 252, 264
PdiI GCCGGC 1 cut(s) 154
PfeI GAWTC 6 cut(s) 211, 284, 305, 756, 933, 1072
PkrI GCNGC 6 cut(s) 169, 182, 280, 323, 886, 1119
PleI GAGTC 1 cut(s) 248
PluTI GGCGCC 1 cut(s) 159
PmaCI CACGTG 1 cut(s) 832
PmlI CACGTG 1 cut(s) 832
PpsI GAGTC 1 cut(s) 248
Ppu21I YACGTR 1 cut(s) 832
Psp124BI GAGCTC 2 cut(s) 51, 1002
Psp6I CCWGG 2 cut(s) 8, 838
PspCI CACGTG 1 cut(s) 832
PspGI CCWGG 2 cut(s) 8, 838
PspN4I GGNNCC 8 cut(s) 6, 147, 148, 157, 293, 332, 447, 837
PspOMI GGGCCC 1 cut(s) 145
PspPI GGNCC 6 cut(s) 133, 145, 146, 323, 330, 632
PstI CTGCAG 1 cut(s) 543
PstNI CAGNNNCTG 1 cut(s) 1123
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 1 cut(s) 457
RsaNI GTAC 1 cut(s) 456
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 2 cut(s) 51, 1002
SaqAI TTAA 3 cut(s) 585, 669, 1130
SatI GCNGC 6 cut(s) 168, 181, 279, 322, 885, 1118
Sau3AI GATC 4 cut(s) 345, 393, 424, 445
Sau96I GGNCC 6 cut(s) 133, 145, 146, 323, 330, 632
SchI GAGTC 1 cut(s) 249
ScrFI CCNGG 3 cut(s) 10, 144, 840
SduI GDGCHC 6 cut(s) 51, 149, 344, 360, 867, 1002
SfaNI GCATC 3 cut(s) 162, 702, 1041
SfcI CTRYAG 3 cut(s) 450, 539, 888
SfoI GGCGCC 1 cut(s) 157
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 1 cut(s) 330
SmiMI CAYNNNNRTG 1 cut(s) 941
Sse9I AATT 5 cut(s) 459, 657, 778, 878, 1021
SspDI GGCGCC 1 cut(s) 155
SspI AATATT 1 cut(s) 805
SstI GAGCTC 2 cut(s) 51, 1002
StyD4I CCNGG 3 cut(s) 8, 142, 838
StyI CCWWGG 3 cut(s) 621, 783, 1112
TaaI ACNGT 1 cut(s) 595
TaiI ACGT 3 cut(s) 30, 42, 834
TaqI TCGA 5 cut(s) 24, 246, 303, 344, 1064
TasI AATT 5 cut(s) 459, 657, 778, 878, 1021
TauI GCSGC 4 cut(s) 170, 183, 281, 324
TfiI GAWTC 6 cut(s) 211, 284, 305, 756, 933, 1072
Tru1I TTAA 3 cut(s) 585, 669, 1130
Tru9I TTAA 3 cut(s) 585, 669, 1130
TscAI CASTG 1 cut(s) 1128
TseFI GTSAC 2 cut(s) 365, 589
TseI GCWGC 2 cut(s) 884, 1117
Tsp45I GTSAC 2 cut(s) 365, 589
TspDTI ATGAA 3 cut(s) 776, 894, 1014
TspGWI ACGGA 1 cut(s) 724
TspRI CASTG 1 cut(s) 1128
VneI GTGCAC 1 cut(s) 863
VpaK11BI GGWCC 1 cut(s) 330
XagI CCTNNNNNAGG 1 cut(s) 491
XapI RAATTY 1 cut(s) 778
XceI RCATGY 1 cut(s) 579
XcmI CCANNNNNNNNNTGG 1 cut(s) 1046
XmiI GTMKAC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.