RLG00000005448

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006732
Physical Location & Seq
Forward (+)
67711990 .. 67714166
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005448

Sequence Viewer

Length: 1020 bp
ATGCACCGCCGCTCCCAGACCTCCTCCTCCTCCGACGAGCCGATTCCGACGCAGGAGTTCATAAGGAGAGTCTTCGAGAACGGAGACGAGGAAGACGACGATTTCGCCGCCGATTCTTGGCTCTGCGCTCTCGATTCAGAAGGCGGTGGCAGCTCTCGGACCCCTTTAGGTTCGATCAAGAAGGGGCTTGGCACTCACAGAGTAGCTAAGGTTGTTGCTATGATCAAATCTTGCACCCCAAATGGTCTTGGTGATCTCATGTTGACTTTGAAGGATCCTACAGGTACAATTGGTGCTACCATCCACCGCAAAGCCCTTTCTGAGGGAGAGTTTGGGAAGAGCATTTCTGTGGGTGCGGTTTTGGTTCTGCAGAAGGTTGCTGTGTTTTCCCCCTCACACACTGCATGTTATCTTAATGTGACGGTGAGCAACATTGTCAAGGTCATTTCCAAGGATAGTGGGCCTCTTGCCACAAAAGATTTTCCAATTTTGTCAGTTAATACTCCTGCTCTCATCAGTGCAGAAAGCAGTAAAATGCTACGGATGCCACAAGAAAAACTCCTCCTGTCACAGGAAAGTACTCAGCGAATCATGAAATGTCTGAGACAAAATTCCAAGGTGATTGGGAGTGACAATATTGAGAAGCATATGGAAACTGGCCATGCGGCACCAGGAAGGATTTTCTCTGCTCATGTGCACTCTGGAAGCCAATTTGCTGCTGTAGAGATAGAGCCTTCATTGGTGGAAAAGGCTATGCCTAATGGAATCACTAAAATGTCTGCTAGAGAGGACATTATTGATACAGAACAAGTAACTCGGGTTGCTGAGGAGCTCAGAATGGCTGAAGAGGACAATTCATCAAGCGTCATCCAACCCATAAGTGGTTCAGCAAACTTGACTGAGATTCCTGATAATCATGGGAGAATAACAGGAGCAAAAAGGCCAAGGCAGCCACTGAGCTTAACAACTGCGCTCCCAGACTATACAGAAGAACAACTAAATCTGTTTGACTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

340

Amino Acids

36.7

Weight (kDa)

5.66

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HROB PF15072 67 - 152 7e-30 Homologous recombination OB-fold protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 667
AccBSI CCGCTC 1 cut(s) 12
AciI CCGC 7 cut(s) 7, 10, 108, 144, 307, 356, 665
AclWI GGATC 2 cut(s) 269, 282
AcoI YGGCCR 1 cut(s) 658
AcsI RAATTY 1 cut(s) 610
AcuI CTGAAG 1 cut(s) 864
AfaI GTAC 2 cut(s) 286, 580
AfiI CCNNNNNNNGG 4 cut(s) 117, 322, 571, 881
AgsI TTSAA 1 cut(s) 271
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 4 cut(s) 153, 206, 832, 960
AluI AGCT 4 cut(s) 153, 206, 832, 960
Alw21I GWGCWC 2 cut(s) 699, 834
Alw26I GTCTC 2 cut(s) 78, 598
Alw44I GTGCAC 1 cut(s) 695
AlwI GGATC 2 cut(s) 269, 282
AlwNI CAGNNNCTG 1 cut(s) 955
Ama87I CYCGRG 1 cut(s) 816
AoxI GGCC 3 cut(s) 461, 658, 941
ApaLI GTGCAC 1 cut(s) 695
ApeKI GCWGC 3 cut(s) 150, 716, 949
ApoI RAATTY 1 cut(s) 610
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AspLEI GCGC 2 cut(s) 128, 973
AspS9I GGNCC 2 cut(s) 159, 461
AsuHPI GGTGA 3 cut(s) 263, 436, 631
AvaI CYCGRG 1 cut(s) 816
AvaII GGWCC 1 cut(s) 159
BaeGI GKGCMC 1 cut(s) 699
BalI TGGCCA 1 cut(s) 660
BamHI GGATCC 1 cut(s) 274
BanI GGYRCC 1 cut(s) 667
BanII GRGCYC 1 cut(s) 834
BbsI GAAGAC 2 cut(s) 64, 99
Bbv12I GWGCWC 2 cut(s) 699, 834
BbvCI CCTCAGC 1 cut(s) 825
BbvI GCAGC 3 cut(s) 162, 703, 961
BccI CCATC 1 cut(s) 308
BciT130I CCWGG 1 cut(s) 672
BclI TGATCA 1 cut(s) 222
BcoDI GTCTC 2 cut(s) 78, 598
BfaI CTAG 1 cut(s) 783
BfmI CTRYAG 3 cut(s) 279, 368, 720
BisI GCNGC 6 cut(s) 10, 108, 151, 666, 717, 950
BlsI GCNGC 6 cut(s) 11, 109, 152, 667, 718, 951
BmcAI AGTACT 1 cut(s) 580
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 1 cut(s) 159
BmeT110I CYCGRG 1 cut(s) 816
BmgT120I GGNCC 2 cut(s) 159, 461
BmiI GGNNCC 3 cut(s) 161, 276, 669
BmrFI CCNGG 1 cut(s) 672
BmsI GCATC 1 cut(s) 534
BpiI GAAGAC 2 cut(s) 64, 99
Bpu10I CCTNAGC 2 cut(s) 207, 825
BsaJI CCNNGG 3 cut(s) 450, 615, 944
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 322, 571, 881
Bse1I ACTGG 1 cut(s) 661
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 3 cut(s) 450, 615, 944
BseGI GGATG 3 cut(s) 300, 549, 867
BseLI CCNNNNNNNGG 4 cut(s) 117, 322, 571, 881
BseMII CTCAG 7 cut(s) 312, 593, 596, 816, 847, 891, 947
BseNI ACTGG 1 cut(s) 661
BseRI GAGGAG 5 cut(s) 13, 16, 19, 551, 842
BseSI GKGCMC 1 cut(s) 699
BseXI GCAGC 3 cut(s) 162, 703, 961
BsgI GTGCAG 1 cut(s) 540
BshFI GGCC 3 cut(s) 463, 660, 943
BshNI GGYRCC 1 cut(s) 667
BsiHKAI GWGCWC 2 cut(s) 699, 834
BsiHKCI CYCGRG 1 cut(s) 816
BslI CCNNNNNNNGG 4 cut(s) 117, 322, 571, 881
BsmAI GTCTC 2 cut(s) 78, 598
BsmBI CGTCTC 1 cut(s) 78
BsnI GGCC 3 cut(s) 463, 660, 943
BsoBI CYCGRG 1 cut(s) 816
Bsp1286I GDGCHC 2 cut(s) 699, 834
Bsp143I GATC 4 cut(s) 174, 222, 253, 274
BspACI CCGC 7 cut(s) 7, 10, 108, 144, 307, 356, 665
BspANI GGCC 3 cut(s) 463, 660, 943
BspCNI CTCAG 7 cut(s) 313, 594, 595, 817, 846, 892, 948
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 3 cut(s) 161, 276, 669
BspMAI CTGCAG 1 cut(s) 372
BspPI GGATC 2 cut(s) 269, 282
BspQI GCTCTTC 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 667
BsrBI CCGCTC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 661
BssECI CCNNGG 3 cut(s) 450, 615, 944
BssMI GATC 4 cut(s) 174, 222, 253, 274
BssT1I CCWWGG 3 cut(s) 450, 615, 944
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 424
Bst6I CTCTTC 2 cut(s) 332, 840
BstDEI CTNAG 8 cut(s) 207, 321, 582, 602, 825, 833, 900, 956
BstENI CCTNNNNNAGG 2 cut(s) 320, 569
BstF5I GGATG 3 cut(s) 300, 549, 867
BstHHI GCGC 2 cut(s) 128, 973
BstKTI GATC 4 cut(s) 177, 225, 256, 277
BstMAI GTCTC 2 cut(s) 78, 598
BstMBI GATC 4 cut(s) 174, 222, 253, 274
BstMWI GCNNNNNNNGC 3 cut(s) 150, 544, 949
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 670
BstSFI CTRYAG 3 cut(s) 279, 368, 720
BstSLI GKGCMC 1 cut(s) 699
BstV1I GCAGC 3 cut(s) 162, 703, 961
BstV2I GAAGAC 2 cut(s) 64, 99
BstX2I RGATCY 1 cut(s) 274
BstYI RGATCY 1 cut(s) 274
BsuRI GGCC 3 cut(s) 463, 660, 943
BtsCI GGATG 3 cut(s) 300, 549, 867
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 3 cut(s) 399, 523, 953
CaiI CAGNNNCTG 1 cut(s) 955
CciI TCATGA 1 cut(s) 591
CfoI GCGC 2 cut(s) 128, 973
Cfr13I GGNCC 2 cut(s) 159, 461
CseI GACGC 2 cut(s) 58, 853
Csp6I GTAC 2 cut(s) 285, 579
CspCI CAANNNNNGTGG 2 cut(s) 439, 474
CviAII CATG 6 cut(s) 259, 405, 592, 662, 692, 917
CviQI GTAC 2 cut(s) 285, 579
DdeI CTNAG 8 cut(s) 207, 321, 582, 602, 825, 833, 900, 956
DpnI GATC 4 cut(s) 176, 224, 255, 276
DpnII GATC 4 cut(s) 174, 222, 253, 274
EaeI YGGCCR 1 cut(s) 658
Eam1104I CTCTTC 2 cut(s) 332, 840
EarI CTCTTC 2 cut(s) 332, 840
Ecl136II GAGCTC 1 cut(s) 832
Eco130I CCWWGG 3 cut(s) 450, 615, 944
Eco24I GRGCYC 1 cut(s) 834
Eco47I GGWCC 1 cut(s) 159
Eco53kI GAGCTC 1 cut(s) 832
Eco57I CTGAAG 1 cut(s) 864
Eco88I CYCGRG 1 cut(s) 816
EcoICRI GAGCTC 1 cut(s) 832
EcoNI CCTNNNNNAGG 2 cut(s) 320, 569
EcoRII CCWGG 1 cut(s) 670
EcoT14I CCWWGG 3 cut(s) 450, 615, 944
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 3 cut(s) 450, 615, 944
Esp3I CGTCTC 1 cut(s) 78
FaeI CATG 6 cut(s) 262, 408, 595, 665, 695, 920
FatI CATG 6 cut(s) 258, 404, 591, 661, 691, 916
FauNDI CATATG 1 cut(s) 648
FbaI TGATCA 1 cut(s) 222
Fnu4HI GCNGC 6 cut(s) 10, 108, 151, 666, 717, 950
FokI GGATG 3 cut(s) 287, 556, 854
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 6 cut(s) 10, 108, 151, 666, 717, 950
FspBI CTAG 1 cut(s) 783
GlaI GCGC 2 cut(s) 127, 972
GluI GCNGC 6 cut(s) 10, 108, 151, 666, 717, 950
HaeIII GGCC 3 cut(s) 463, 660, 943
HgaI GACGC 2 cut(s) 58, 853
HhaI GCGC 2 cut(s) 128, 973
Hin1II CATG 6 cut(s) 262, 408, 595, 665, 695, 920
Hin6I GCGC 2 cut(s) 126, 971
HinP1I GCGC 2 cut(s) 126, 971
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 7 cut(s) 43, 69, 113, 134, 588, 765, 904
HphI GGTGA 3 cut(s) 263, 436, 631
Hpy166II GTNNAC 2 cut(s) 264, 697
Hpy188I TCNGA 7 cut(s) 34, 48, 139, 159, 322, 603, 836
Hpy188III TCNNGA 6 cut(s) 76, 131, 178, 592, 702, 908
Hpy8I GTNNAC 2 cut(s) 264, 697
Hpy99I CGWCG 3 cut(s) 38, 52, 101
HpyAV CCTTC 6 cut(s) 134, 175, 265, 367, 669, 744
HpyCH4III ACNGT 1 cut(s) 424
HpyCH4V TGCA 6 cut(s) 4, 234, 370, 404, 521, 697
HpyF10VI GCNNNNNNNGC 3 cut(s) 150, 544, 949
HpyF3I CTNAG 8 cut(s) 207, 321, 582, 602, 825, 833, 900, 956
Hsp92II CATG 6 cut(s) 262, 408, 595, 665, 695, 920
HspAI GCGC 2 cut(s) 126, 971
Ksp22I TGATCA 1 cut(s) 222
Kzo9I GATC 4 cut(s) 174, 222, 253, 274
LguI GCTCTTC 1 cut(s) 332
LmnI GCTCC 4 cut(s) 17, 829, 932, 978
Lsp1109I GCAGC 3 cut(s) 162, 703, 961
LweI GCATC 1 cut(s) 534
MaeI CTAG 1 cut(s) 783
MaeIII GTNAC 4 cut(s) 418, 567, 629, 811
MalI GATC 4 cut(s) 176, 224, 255, 276
MbiI CCGCTC 1 cut(s) 12
MboI GATC 4 cut(s) 174, 222, 253, 274
MboII GAAGA 5 cut(s) 64, 104, 349, 857, 1001
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 1 cut(s) 274
MhlI GDGCHC 2 cut(s) 699, 834
MlsI TGGCCA 1 cut(s) 660
MluCI AATT 5 cut(s) 288, 486, 610, 710, 853
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 1 cut(s) 78
MmeI TCCRAC 3 cut(s) 57, 71, 895
Mox20I TGGCCA 1 cut(s) 660
MscI TGGCCA 1 cut(s) 660
MseI TTAA 3 cut(s) 414, 498, 962
MslI CAYNNNNRTG 2 cut(s) 347, 773
Msp20I TGGCCA 1 cut(s) 660
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 1 cut(s) 288
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 3 cut(s) 150, 544, 949
NdeI CATATG 1 cut(s) 648
NdeII GATC 4 cut(s) 174, 222, 253, 274
NlaIII CATG 6 cut(s) 262, 408, 595, 665, 695, 920
NlaIV GGNNCC 3 cut(s) 161, 276, 669
NmuCI GTSAC 3 cut(s) 418, 567, 629
NspI RCATGY 1 cut(s) 408
PagI TCATGA 1 cut(s) 591
PciSI GCTCTTC 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 93
PfeI GAWTC 6 cut(s) 43, 113, 134, 588, 765, 904
PkrI GCNGC 6 cut(s) 11, 109, 152, 667, 718, 951
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Psp124BI GAGCTC 1 cut(s) 834
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 161, 276, 669
PspPI GGNCC 2 cut(s) 159, 461
PstI CTGCAG 1 cut(s) 372
PstNI CAGNNNCTG 1 cut(s) 955
PsuI RGATCY 1 cut(s) 274
RsaI GTAC 2 cut(s) 286, 580
RsaNI GTAC 2 cut(s) 285, 579
RseI CAYNNNNRTG 2 cut(s) 347, 773
SacI GAGCTC 1 cut(s) 834
SapI GCTCTTC 1 cut(s) 332
SaqAI TTAA 3 cut(s) 414, 498, 962
SatI GCNGC 6 cut(s) 10, 108, 151, 666, 717, 950
Sau3AI GATC 4 cut(s) 174, 222, 253, 274
Sau96I GGNCC 2 cut(s) 159, 461
ScaI AGTACT 1 cut(s) 580
SchI GAGTC 1 cut(s) 78
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 2 cut(s) 699, 834
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 3 cut(s) 279, 368, 720
SinI GGWCC 1 cut(s) 159
SmiMI CAYNNNNRTG 2 cut(s) 347, 773
Sse9I AATT 5 cut(s) 288, 486, 610, 710, 853
SsiI CCGC 7 cut(s) 7, 10, 108, 144, 307, 356, 665
SspI AATATT 1 cut(s) 637
SspMI CTAG 1 cut(s) 783
SstI GAGCTC 1 cut(s) 834
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 3 cut(s) 450, 615, 944
TaaI ACNGT 1 cut(s) 424
TaqI TCGA 3 cut(s) 75, 132, 173
TasI AATT 5 cut(s) 288, 486, 610, 710, 853
TatI WGTACW 1 cut(s) 578
TauI GCSGC 3 cut(s) 12, 110, 668
TfiI GAWTC 6 cut(s) 43, 113, 134, 588, 765, 904
Tru1I TTAA 3 cut(s) 414, 498, 962
Tru9I TTAA 3 cut(s) 414, 498, 962
TscAI CASTG 3 cut(s) 406, 523, 960
TseFI GTSAC 3 cut(s) 418, 567, 629
TseI GCWGC 3 cut(s) 150, 716, 949
Tsp45I GTSAC 3 cut(s) 418, 567, 629
TspDTI ATGAA 4 cut(s) 49, 608, 726, 846
TspGWI ACGGA 2 cut(s) 96, 556
TspRI CASTG 3 cut(s) 406, 523, 960
VneI GTGCAC 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 159
XagI CCTNNNNNAGG 2 cut(s) 320, 569
XapI RAATTY 1 cut(s) 610
XceI RCATGY 1 cut(s) 408
XcmI CCANNNNNNNNNTGG 1 cut(s) 878
XspI CTAG 1 cut(s) 783
ZrmI AGTACT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.