Prupe.5G233500_v2.0.a1

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0006732
Physical Location & Seq
Forward (+)
17743679 .. 17746857
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G233500.1

Sequence Viewer

Length: 1317 bp
ATGGATGAGCATGAACCCTGGGAACAACTAGACGTCGACGAATCAGAGCTCATCTCTCTTAGTCTTCGTCCTTGCAAGCGCTCTGCTTCTCATTCCCTCAAATCAAATCATCCTCAGCCCCCCAAGCCCACTTCCAACCTCATTCCGGGCCCCGCCGGAGCCCTACAGGCCGCGATGCAGCGCAGGACCCAAACCCACCGCCACCACCCCCACGACCGCGATAAGGAGGAGCCCATTCCGACCCAAGAGTTCGTAAGGAGAGTCGTCGAGGTTGGCCATGATCACGATGCCGATTTCACCACCAATCCCTGGCTTTGCACTCTCGATTTTCTTACCAAACAAGGTGCTCACTTTCCGACGACCTTGGGTTCTATCAGGAAGGGGATTGAATCTCACAGATTAGCTCAGGTTGTTGCTATAATCAAATCTTGCACCCCAAATGGTCTTGGTGATCTCATGCTCACTCTGAAGGATCCTACAGGTACAATTGGTGCTAGCATCCACCACACAGTCCTTTCTGAGGGAGACTTTGGGAAGAGCATATCTGTTGGTGCAGTTTTGGTACTGCAGAAGGTTGCTGTGTTCACCCCCTCACGATCTGCATGTTATCTTAATATAACCAAGAACAACATGGTTAAGGTCATCTCCAAGGATAGTGGACCTCTCGTGACTAAAGATCTTCTCACCTCATCAGTCCAGAATCCTGCTCCCAGCAGTGGAAGTAGTGAAAACTTATGGATGCCACAAGAAAAGTTCCCTCCGTCACAGGAAAGCACTGAAAGAATCATGAATTTTCTGAGACAAAACTCTAAGGCGAGAGAGAGTCAGCATAATACTGAGAAACACATGGAAACAGATCATGCGGCAGCAGGGCTGAGCTGCTCTGGCCACGAGCAGTGCGGAAGCCGAAACACGGACGTAGAGGTTCTATCTTCATTGGTGAAAAAGGCAATGCCTGATGGAATCACAAACATGGAGGACACTACTGATAACGATCAAGTAACTGTGGTAGCTGAGAAGCCCAACGCTGGCAGAGTGGCTGATGGAGACAATGCATCCAGCCACACCCAAGCCATTAATCATGTAACTGTGGTAACTGAGAAGCCCAAACATGGCACGGTGGCCAAAGGTTACAATCCATTGAGCAGGACGCAACCCATTAGTGCTTCAGCAAACTCAGTTGAGATCCCTAACCCTGACGATGATCAAGAAGTTGAGAGAATAACTGGAGCTAAAAGGCAGAGGCAGCCACTAATTTCGAGAACTTCACTCCCAGAGTATACAGAAGAAGAGCTGGATCTTTTTGAATTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

439

Amino Acids

48.06

Weight (kDa)

5.85

Isoelectric Point (pI)

42.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 36
AccB7I CCANNNNNTGG 1 cut(s) 309
AccI GTMKAC 2 cut(s) 36, 1280
AccII CGCG 2 cut(s) 173, 219
AciI CCGC 6 cut(s) 153, 171, 199, 217, 863, 900
AclWI GGATC 4 cut(s) 467, 480, 1180, 1305
AcoI YGGCCR 3 cut(s) 274, 886, 1122
AcsI RAATTY 2 cut(s) 790, 1307
AcuI CTGAAG 2 cut(s) 488, 1152
AcyI GRCGYC 1 cut(s) 33
AfaI GTAC 2 cut(s) 484, 564
AfeI AGCGCT 1 cut(s) 80
AfiI CCNNNNNNNGG 8 cut(s) 145, 223, 309, 520, 716, 913, 1028, 1112
AgsI TTSAA 2 cut(s) 389, 1307
AjnI CCWGG 2 cut(s) 17, 308
AluBI AGCT 6 cut(s) 49, 404, 879, 1013, 1232, 1294
AluI AGCT 6 cut(s) 49, 404, 879, 1013, 1232, 1294
Alw21I GWGCWC 2 cut(s) 51, 349
Alw26I GTCTC 3 cut(s) 519, 793, 1041
AlwI GGATC 4 cut(s) 467, 480, 1180, 1305
Aor51HI AGCGCT 1 cut(s) 80
AoxI GGCC 5 cut(s) 148, 168, 274, 886, 1122
ApaI GGGCCC 1 cut(s) 152
ApeKI GCWGC 4 cut(s) 178, 866, 879, 1246
ApoI RAATTY 2 cut(s) 790, 1307
AseI ATTAAT 1 cut(s) 1077
AspLEI GCGC 2 cut(s) 81, 183
AspS9I GGNCC 4 cut(s) 148, 149, 186, 659
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 5 cut(s) 289, 461, 577, 676, 952
AsuNHI GCTAGC 1 cut(s) 494
AvaII GGWCC 2 cut(s) 186, 659
BaeGI GKGCMC 1 cut(s) 152
BalI TGGCCA 3 cut(s) 276, 888, 1124
BamHI GGATCC 1 cut(s) 472
BanII GRGCYC 4 cut(s) 51, 152, 163, 234
BauI CACGAG 2 cut(s) 665, 890
BbsI GAAGAC 1 cut(s) 56
Bbv12I GWGCWC 2 cut(s) 51, 349
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 4 cut(s) 190, 866, 878, 1258
BccI CCATC 2 cut(s) 953, 1037
BciT130I CCWGG 2 cut(s) 19, 310
BclI TGATCA 2 cut(s) 280, 1204
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 3 cut(s) 519, 793, 1041
BfaI CTAG 2 cut(s) 29, 495
BfmI CTRYAG 3 cut(s) 164, 477, 566
BfoI RGCGCY 1 cut(s) 82
BglI GCCNNNNNGGC 1 cut(s) 167
BglII AGATCT 1 cut(s) 676
BisI GCNGC 6 cut(s) 171, 179, 864, 867, 880, 1247
BlpI GCTNAGC 1 cut(s) 875
BlsI GCNGC 6 cut(s) 172, 180, 865, 868, 881, 1248
Bme1390I CCNGG 3 cut(s) 19, 147, 310
Bme18I GGWCC 2 cut(s) 186, 659
BmgT120I GGNCC 4 cut(s) 148, 149, 186, 659
BmiI GGNNCC 6 cut(s) 150, 151, 160, 188, 231, 474
BmrFI CCNGG 3 cut(s) 19, 147, 310
BmsI GCATC 5 cut(s) 165, 277, 507, 729, 1064
BmtI GCTAGC 1 cut(s) 498
BpiI GAAGAC 1 cut(s) 56
BplI GAGNNNNNCTC 2 cut(s) 38, 70
BpmI CTGGAG 1 cut(s) 1248
Bpu10I CCTNAGC 2 cut(s) 114, 405
Bpu1102I GCTNAGC 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 147
BsaBI GATNNNNATC 1 cut(s) 993
BsaHI GRCGYC 1 cut(s) 33
BsaJI CCNNGG 5 cut(s) 17, 18, 308, 363, 648
Bsc4I CCNNNNNNNGG 8 cut(s) 145, 223, 309, 520, 716, 913, 1028, 1112
Bse1I ACTGG 1 cut(s) 1231
Bse3DI GCAATG 1 cut(s) 957
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 2 cut(s) 19, 310
BseDI CCNNGG 5 cut(s) 17, 18, 308, 363, 648
BseGI GGATG 5 cut(s) 10, 109, 498, 744, 1055
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 8 cut(s) 145, 223, 309, 520, 716, 913, 1028, 1112
BseMI GCAATG 1 cut(s) 957
BseMII CTCAG 9 cut(s) 128, 419, 510, 788, 828, 866, 1005, 1089, 1191
BseNI ACTGG 1 cut(s) 1231
BseRI GAGGAG 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 152
BseXI GCAGC 4 cut(s) 190, 866, 878, 1258
BseYI CCCAGC 1 cut(s) 710
BsgI GTGCAG 1 cut(s) 573
Bsh1236I CGCG 2 cut(s) 173, 219
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 5 cut(s) 150, 170, 276, 888, 1124
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 2 cut(s) 51, 349
BsiSI CCGG 2 cut(s) 146, 156
BslI CCNNNNNNNGG 8 cut(s) 145, 223, 309, 520, 716, 913, 1028, 1112
BsmAI GTCTC 3 cut(s) 519, 793, 1041
BsnI GGCC 5 cut(s) 150, 170, 276, 888, 1124
Bsp120I GGGCCC 1 cut(s) 148
Bsp1286I GDGCHC 5 cut(s) 51, 152, 163, 234, 349
Bsp1720I GCTNAGC 1 cut(s) 875
BspACI CCGC 6 cut(s) 153, 171, 199, 217, 863, 900
BspANI GGCC 5 cut(s) 150, 170, 276, 888, 1124
BspCNI CTCAG 9 cut(s) 127, 418, 511, 789, 829, 867, 1006, 1090, 1190
BspFNI CGCG 2 cut(s) 173, 219
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 6 cut(s) 150, 151, 160, 188, 231, 474
BspMAI CTGCAG 1 cut(s) 570
BspOI GCTAGC 1 cut(s) 498
BspPI GGATC 4 cut(s) 467, 480, 1180, 1305
BspQI GCTCTTC 2 cut(s) 530, 1284
BsrDI GCAATG 1 cut(s) 957
BsrI ACTGG 1 cut(s) 1231
BssECI CCNNGG 5 cut(s) 17, 18, 308, 363, 648
BssNAI GTATAC 1 cut(s) 1281
BssNI GRCGYC 1 cut(s) 33
BssSI CACGAG 2 cut(s) 665, 890
BssT1I CCWWGG 2 cut(s) 363, 648
Bst1107I GTATAC 1 cut(s) 1281
Bst2BI CACGAG 2 cut(s) 665, 890
Bst2UI CCWGG 2 cut(s) 19, 310
Bst4CI ACNGT 4 cut(s) 511, 1006, 1090, 1120
Bst6I CTCTTC 2 cut(s) 530, 1284
BstACI GRCGYC 1 cut(s) 33
BstC8I GCNNGC 3 cut(s) 77, 496, 1030
BstENI CCTNNNNNAGG 1 cut(s) 518
BstF5I GGATG 5 cut(s) 10, 109, 498, 744, 1055
BstFNI CGCG 2 cut(s) 173, 219
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 2 cut(s) 81, 183
BstMAI GTCTC 3 cut(s) 519, 793, 1041
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 4 cut(s) 124, 167, 885, 1246
BstNI CCWGG 2 cut(s) 19, 310
BstNSI RCATGY 1 cut(s) 606
BstSCI CCNGG 3 cut(s) 17, 145, 308
BstSFI CTRYAG 3 cut(s) 164, 477, 566
BstSLI GKGCMC 1 cut(s) 152
BstUI CGCG 2 cut(s) 173, 219
BstV1I GCAGC 4 cut(s) 190, 866, 878, 1258
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 4 cut(s) 472, 676, 1185, 1297
BstYI RGATCY 4 cut(s) 472, 676, 1185, 1297
BstZ17I GTATAC 1 cut(s) 1281
BsuRI GGCC 5 cut(s) 150, 170, 276, 888, 1124
BtgZI GCGATG 1 cut(s) 188
BtsCI GGATG 5 cut(s) 10, 109, 498, 744, 1055
BtsI GCAGTG 2 cut(s) 721, 902
BtsIMutI CAGTG 3 cut(s) 721, 774, 902
Cac8I GCNNGC 3 cut(s) 77, 496, 1030
CciI TCATGA 1 cut(s) 786
CfoI GCGC 2 cut(s) 81, 183
Cfr13I GGNCC 4 cut(s) 148, 149, 186, 659
CseI GACGC 1 cut(s) 1159
Csp6I GTAC 2 cut(s) 483, 563
CspCI CAANNNNNGTGG 2 cut(s) 637, 672
CviQI GTAC 2 cut(s) 483, 563
EaeI YGGCCR 3 cut(s) 274, 886, 1122
Eam1104I CTCTTC 2 cut(s) 530, 1284
EarI CTCTTC 2 cut(s) 530, 1284
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 363, 648
Eco24I GRGCYC 4 cut(s) 51, 152, 163, 234
Eco47I GGWCC 2 cut(s) 186, 659
Eco47III AGCGCT 1 cut(s) 80
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 2 cut(s) 488, 1152
EcoICRI GAGCTC 1 cut(s) 49
EcoNI CCTNNNNNAGG 1 cut(s) 518
EcoO109I RGGNCCY 2 cut(s) 149, 186
EcoRII CCWGG 2 cut(s) 17, 308
EcoT14I CCWWGG 2 cut(s) 363, 648
EcoT22I ATGCAT 1 cut(s) 1057
EcoT38I GRGCYC 4 cut(s) 51, 152, 163, 234
ErhI CCWWGG 2 cut(s) 363, 648
FauI CCCGC 1 cut(s) 160
FbaI TGATCA 2 cut(s) 280, 1204
FblI GTMKAC 2 cut(s) 36, 1280
Fnu4HI GCNGC 6 cut(s) 171, 179, 864, 867, 880, 1247
FokI GGATG 5 cut(s) 17, 96, 485, 751, 1042
FriOI GRGCYC 4 cut(s) 51, 152, 163, 234
Fsp4HI GCNGC 6 cut(s) 171, 179, 864, 867, 880, 1247
FspBI CTAG 2 cut(s) 29, 495
GlaI GCGC 2 cut(s) 80, 182
GluI GCNGC 6 cut(s) 171, 179, 864, 867, 880, 1247
GsaI CCCAGC 1 cut(s) 714
GsuI CTGGAG 1 cut(s) 1248
HaeII RGCGCY 1 cut(s) 82
HaeIII GGCC 5 cut(s) 150, 170, 276, 888, 1124
HapII CCGG 2 cut(s) 146, 156
HgaI GACGC 1 cut(s) 1159
HhaI GCGC 2 cut(s) 81, 183
Hin1I GRCGYC 1 cut(s) 33
Hin6I GCGC 2 cut(s) 79, 181
HinP1I GCGC 2 cut(s) 79, 181
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 7 cut(s) 41, 261, 389, 700, 783, 823, 963
HpaII CCGG 2 cut(s) 146, 156
HphI GGTGA 5 cut(s) 289, 461, 577, 676, 952
Hpy166II GTNNAC 4 cut(s) 37, 585, 659, 1281
Hpy188I TCNGA 6 cut(s) 46, 240, 357, 468, 520, 798
Hpy188III TCNNGA 9 cut(s) 284, 323, 376, 594, 667, 697, 787, 1208, 1260
Hpy8I GTNNAC 4 cut(s) 37, 585, 659, 1281
Hpy99I CGWCG 4 cut(s) 38, 41, 269, 361
HpyAV CCTTC 3 cut(s) 373, 463, 565
HpyCH4III ACNGT 4 cut(s) 511, 1006, 1090, 1120
HpyCH4IV ACGT 2 cut(s) 33, 918
HpyCH4V TGCA 8 cut(s) 75, 178, 318, 432, 554, 568, 602, 1055
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 167, 885, 1246
HpySE526I ACGT 2 cut(s) 33, 918
Hsp92I GRCGYC 1 cut(s) 33
HspAI GCGC 2 cut(s) 79, 181
Ksp22I TGATCA 2 cut(s) 280, 1204
LguI GCTCTTC 2 cut(s) 530, 1284
LmnI GCTCC 4 cut(s) 158, 229, 712, 1229
Lsp1109I GCAGC 4 cut(s) 190, 866, 878, 1258
LweI GCATC 5 cut(s) 165, 277, 507, 729, 1064
MaeI CTAG 2 cut(s) 29, 495
MaeII ACGT 2 cut(s) 33, 918
MaeIII GTNAC 6 cut(s) 667, 762, 1000, 1084, 1093, 1130
MboII GAAGA 6 cut(s) 56, 547, 671, 924, 1298, 1301
MfeI CAATTG 1 cut(s) 486
MflI RGATCY 4 cut(s) 472, 676, 1185, 1297
MhlI GDGCHC 5 cut(s) 51, 152, 163, 234, 349
MlsI TGGCCA 3 cut(s) 276, 888, 1124
MluCI AATT 4 cut(s) 486, 790, 1254, 1307
MluNI TGGCCA 3 cut(s) 276, 888, 1124
MlyI GAGTC 2 cut(s) 270, 832
MmeI TCCRAC 3 cut(s) 159, 263, 380
Mox20I TGGCCA 3 cut(s) 276, 888, 1124
Mph1103I ATGCAT 1 cut(s) 1057
MscI TGGCCA 3 cut(s) 276, 888, 1124
MseI TTAA 3 cut(s) 612, 636, 1077
MslI CAYNNNNRTG 1 cut(s) 971
Msp20I TGGCCA 3 cut(s) 276, 888, 1124
MspI CCGG 2 cut(s) 146, 156
MspR9I CCNGG 3 cut(s) 19, 147, 310
MunI CAATTG 1 cut(s) 486
MvaI CCWGG 2 cut(s) 19, 310
MvnI CGCG 2 cut(s) 173, 219
MwoI GCNNNNNNNGC 4 cut(s) 124, 167, 885, 1246
NciI CCSGG 1 cut(s) 147
NheI GCTAGC 1 cut(s) 494
NlaIV GGNNCC 6 cut(s) 150, 151, 160, 188, 231, 474
NmuCI GTSAC 2 cut(s) 667, 762
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 1 cut(s) 606
PagI TCATGA 1 cut(s) 786
PasI CCCWGGG 1 cut(s) 18
PciSI GCTCTTC 2 cut(s) 530, 1284
PfeI GAWTC 5 cut(s) 41, 389, 700, 783, 963
PflMI CCANNNNNTGG 1 cut(s) 309
PkrI GCNGC 6 cut(s) 172, 180, 865, 868, 881, 1248
PleI GAGTC 2 cut(s) 269, 831
PpsI GAGTC 2 cut(s) 269, 831
PpuMI RGGWCCY 1 cut(s) 186
PshBI ATTAAT 1 cut(s) 1077
Psp124BI GAGCTC 1 cut(s) 51
Psp5II RGGWCCY 1 cut(s) 186
Psp6I CCWGG 2 cut(s) 17, 308
PspFI CCCAGC 1 cut(s) 710
PspGI CCWGG 2 cut(s) 17, 308
PspN4I GGNNCC 6 cut(s) 150, 151, 160, 188, 231, 474
PspOMI GGGCCC 1 cut(s) 148
PspPI GGNCC 4 cut(s) 148, 149, 186, 659
PspPPI RGGWCCY 1 cut(s) 186
PstI CTGCAG 1 cut(s) 570
PsuI RGATCY 4 cut(s) 472, 676, 1185, 1297
RsaI GTAC 2 cut(s) 484, 564
RsaNI GTAC 2 cut(s) 483, 563
RseI CAYNNNNRTG 1 cut(s) 971
SacI GAGCTC 1 cut(s) 51
SalI GTCGAC 1 cut(s) 35
SapI GCTCTTC 2 cut(s) 530, 1284
SaqAI TTAA 3 cut(s) 612, 636, 1077
SatI GCNGC 6 cut(s) 171, 179, 864, 867, 880, 1247
Sau96I GGNCC 4 cut(s) 148, 149, 186, 659
SchI GAGTC 2 cut(s) 270, 832
ScrFI CCNGG 3 cut(s) 19, 147, 310
SduI GDGCHC 5 cut(s) 51, 152, 163, 234, 349
SfaNI GCATC 5 cut(s) 165, 277, 507, 729, 1064
SfcI CTRYAG 3 cut(s) 164, 477, 566
SgrDI CGTCGACG 1 cut(s) 35
SinI GGWCC 2 cut(s) 186, 659
SmiMI CAYNNNNRTG 1 cut(s) 971
Sse9I AATT 4 cut(s) 486, 790, 1254, 1307
SsiI CCGC 6 cut(s) 153, 171, 199, 217, 863, 900
SspMI CTAG 2 cut(s) 29, 495
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 17, 145, 308
StyI CCWWGG 2 cut(s) 363, 648
TaaI ACNGT 4 cut(s) 511, 1006, 1090, 1120
TaiI ACGT 2 cut(s) 36, 921
TaqI TCGA 4 cut(s) 36, 267, 324, 1259
TasI AATT 4 cut(s) 486, 790, 1254, 1307
TauI GCSGC 2 cut(s) 173, 866
TfiI GAWTC 5 cut(s) 41, 389, 700, 783, 963
Tru1I TTAA 3 cut(s) 612, 636, 1077
Tru9I TTAA 3 cut(s) 612, 636, 1077
TscAI CASTG 3 cut(s) 721, 781, 902
TseFI GTSAC 2 cut(s) 667, 762
TseI GCWGC 4 cut(s) 178, 866, 879, 1246
Tsp45I GTSAC 2 cut(s) 667, 762
TspDTI ATGAA 3 cut(s) 27, 803, 924
TspGWI ACGGA 2 cut(s) 750, 929
TspRI CASTG 3 cut(s) 721, 781, 902
Van91I CCANNNNNTGG 1 cut(s) 309
VpaK11BI GGWCC 2 cut(s) 186, 659
VspI ATTAAT 1 cut(s) 1077
XagI CCTNNNNNAGG 1 cut(s) 518
XapI RAATTY 2 cut(s) 790, 1307
XceI RCATGY 1 cut(s) 606
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XmiI GTMKAC 2 cut(s) 36, 1280
XspI CTAG 2 cut(s) 29, 495
ZraI GACGTC 1 cut(s) 34
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.