FvH4_5g17330

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006732
Physical Location & Seq
Forward (+)
9905283 .. 9907906
2624 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g17330.t1

Sequence Viewer

Length: 1176 bp
ATGGAACCCTGGGAAGAACAACTCGACATAAACGACGACGTTTCAGAGCTCCCATCCTTCTCTCTCCGCCCTTGCAACCACCGCGCTTCGTCTCCTTCCCCAAATCCCCAATCCAAACCCTCCTCTTCATCTCCGCCCCTTATTCCAGGACCCGCTAGCGCCGTTCAGGCCGCGATGCACCGCCGCTCCCATACCCACTCCGACGAGCCGGTTCCGACGCAGGAGTTAAGGAGAGTCGTCGAGAACGGAGATGAGGAAGACGACGATTTCGCCGCCGATTCTTGGCTCCGCGCTCTCGAATTCGGAGGCGGCGGCTCTCGGACCCCTTTGGGCTCCATCAAGAAGGGGCTTGACACTCTCAGAGTAGCTAAGGTTGTTGCTATGATCAAATCTTGCACCCCAAATGGTCTTGGTGATCTCATGTTGACTTTGAAGGATCCTACAGGTACAATTGGTGCTACCATCCACCGCAAAGCCCTTTCCGAGGGAGAGTTTGGGAAGAGCATTTCTGTTGGTGCAGTTTTGGTTCTGCAGAAGGTGGTCGTGTTTTCTCCCTCGCGCTCTGCTTGTTATCTTAATGTGACTTTGAACAACATTGTCAAGGTAATTTCCAAGGACAGTGGACTTCTTGCCACAAAAGAATTTCCAATTTCACCAGTCAATACTCCTGCTCCCATCAGTGAAAGTAGTAAAACGTTACGGATGGCACAAGAAACATCCCTCACGGCACAAAGTACACAGCGAATCATGAAATATCTGAGACAAAATACCAATGTGACTGGGAGTGAAAATACTGAGAAGCATATGGAAGTTGGCCACACGGCACCTGGAAGGATTTTATCTGGTCATGCCCACTCTGCAAGCCAATATGCTGCTGTAGAGATGGAACCTTCATTGGTGGAAAAGGCTATGCCTAATAGAATCAATAAAATGTCAGTTAGAGAAGACGTTATTGATACCGAATATCTAACAGGGGTAGCTGAGGAGATCAGAATGGCTGAAGAGGACAATTCATCAAGCATCAGCCAATGCATTAGTGGTTCAGCAAACTCAACTGAGATTCCTGATAATCATGGGAGAATAACAGGAGCAAAACGGCCAAGGCAGCCACTGAGCTTAACAACTGCACTCCCAGACTATACAGAAGAACAACTAAATCAGTTTGATTTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

392

Amino Acids

42.36

Weight (kDa)

5.48

Isoelectric Point (pI)

60.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HROB PF15072 121 - 206 1.2e-29 Homologous recombination OB-fold protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 823
AccBSI CCGCTC 1 cut(s) 186
AccII CGCG 4 cut(s) 84, 173, 291, 559
AclI AACGTT 1 cut(s) 695
AclWI GGATC 2 cut(s) 431, 444
AcoI YGGCCR 2 cut(s) 814, 1097
AcsI RAATTY 2 cut(s) 299, 641
AcuI CTGAAG 1 cut(s) 1020
AfaI GTAC 2 cut(s) 448, 736
AfiI CCNNNNNNNGG 2 cut(s) 282, 484
AgsI TTSAA 2 cut(s) 433, 589
AjnI CCWGG 3 cut(s) 8, 145, 826
AluBI AGCT 4 cut(s) 49, 368, 980, 1116
AluI AGCT 4 cut(s) 49, 368, 980, 1116
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 2 cut(s) 96, 754
AlwI GGATC 2 cut(s) 431, 444
AlwNI CAGNNNCTG 1 cut(s) 1111
AoxI GGCC 3 cut(s) 168, 814, 1097
ApeKI GCWGC 2 cut(s) 872, 1105
ApoI RAATTY 2 cut(s) 299, 641
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AspLEI GCGC 4 cut(s) 86, 161, 293, 561
AspS9I GGNCC 2 cut(s) 149, 321
AsuHPI GGTGA 2 cut(s) 425, 645
AsuNHI GCTAGC 1 cut(s) 155
AvaII GGWCC 2 cut(s) 149, 321
BalI TGGCCA 1 cut(s) 816
BamHI GGATCC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 2 cut(s) 51, 335
BbsI GAAGAC 2 cut(s) 264, 951
Bbv12I GWGCWC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 981
BbvI GCAGC 2 cut(s) 859, 1117
BccI CCATC 6 cut(s) 61, 344, 470, 683, 697, 877
BceAI ACGGC 4 cut(s) 146, 741, 837, 1112
BciT130I CCWGG 3 cut(s) 10, 147, 828
BclI TGATCA 1 cut(s) 384
BcoDI GTCTC 2 cut(s) 96, 754
BfaI CTAG 1 cut(s) 156
BfmI CTRYAG 3 cut(s) 441, 530, 876
BfoI RGCGCY 1 cut(s) 162
BglI GCCNNNNNGGC 1 cut(s) 167
BisI GCNGC 7 cut(s) 171, 184, 273, 310, 313, 873, 1106
BlsI GCNGC 7 cut(s) 172, 185, 274, 311, 314, 874, 1107
Bme1390I CCNGG 3 cut(s) 10, 147, 828
Bme18I GGWCC 2 cut(s) 149, 321
BmgT120I GGNCC 2 cut(s) 149, 321
BmiI GGNNCC 9 cut(s) 6, 151, 213, 287, 323, 334, 438, 825, 888
BmrFI CCNGG 3 cut(s) 10, 147, 828
BmrI ACTGGG 1 cut(s) 789
BmsI GCATC 2 cut(s) 165, 1029
BmtI GCTAGC 1 cut(s) 159
BmuI ACTGGG 1 cut(s) 789
BpiI GAAGAC 2 cut(s) 264, 951
Bpu10I CCTNAGC 2 cut(s) 369, 981
BsaJI CCNNGG 5 cut(s) 8, 9, 483, 612, 1100
BsaXI ACNNNNNCTCC 4 cut(s) 170, 200, 655, 685
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 484
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 656, 784
BseBI CCWGG 3 cut(s) 10, 147, 828
BseDI CCNNGG 5 cut(s) 8, 9, 483, 612, 1100
BseGI GGATG 4 cut(s) 53, 462, 708, 716
BseLI CCNNNNNNNGG 2 cut(s) 282, 484
BseMII CTCAG 6 cut(s) 373, 749, 786, 972, 1047, 1103
BseNI ACTGG 2 cut(s) 656, 784
BseRI GAGGAG 2 cut(s) 112, 998
BseXI GCAGC 2 cut(s) 859, 1117
BsgI GTGCAG 2 cut(s) 537, 1110
Bsh1236I CGCG 4 cut(s) 84, 173, 291, 559
BshFI GGCC 3 cut(s) 170, 816, 1099
BshNI GGYRCC 1 cut(s) 823
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 2 cut(s) 282, 484
BsmAI GTCTC 2 cut(s) 96, 754
BsmBI CGTCTC 1 cut(s) 96
BsnI GGCC 3 cut(s) 170, 816, 1099
Bsp1286I GDGCHC 2 cut(s) 51, 335
Bsp143I GATC 4 cut(s) 384, 415, 436, 987
BspANI GGCC 3 cut(s) 170, 816, 1099
BspCNI CTCAG 6 cut(s) 372, 750, 787, 973, 1048, 1104
BspFNI CGCG 4 cut(s) 84, 173, 291, 559
BspHI TCATGA 1 cut(s) 747
BspLI GGNNCC 9 cut(s) 6, 151, 213, 287, 323, 334, 438, 825, 888
BspMAI CTGCAG 1 cut(s) 534
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 2 cut(s) 431, 444
BspQI GCTCTTC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 823
BsrBI CCGCTC 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 2 cut(s) 656, 784
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 5 cut(s) 8, 9, 483, 612, 1100
BssMI GATC 4 cut(s) 384, 415, 436, 987
BssT1I CCWWGG 2 cut(s) 612, 1100
Bst2UI CCWGG 3 cut(s) 10, 147, 828
Bst4CI ACNGT 1 cut(s) 620
Bst6I CTCTTC 3 cut(s) 130, 494, 996
BstC8I GCNNGC 2 cut(s) 157, 862
BstDEI CTNAG 7 cut(s) 359, 369, 758, 795, 981, 1056, 1112
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 4 cut(s) 53, 462, 708, 716
BstFNI CGCG 4 cut(s) 84, 173, 291, 559
BstH2I RGCGCY 1 cut(s) 162
BstHHI GCGC 4 cut(s) 86, 161, 293, 561
BstKTI GATC 4 cut(s) 387, 418, 439, 990
BstMAI GTCTC 2 cut(s) 96, 754
BstMBI GATC 4 cut(s) 384, 415, 436, 987
BstMWI GCNNNNNNNGC 4 cut(s) 81, 167, 857, 1105
BstNI CCWGG 3 cut(s) 10, 147, 828
BstSCI CCNGG 3 cut(s) 8, 145, 826
BstSFI CTRYAG 3 cut(s) 441, 530, 876
BstUI CGCG 4 cut(s) 84, 173, 291, 559
BstV1I GCAGC 2 cut(s) 859, 1117
BstV2I GAAGAC 2 cut(s) 264, 951
BstX2I RGATCY 1 cut(s) 436
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 3 cut(s) 170, 816, 1099
BtgZI GCGATG 1 cut(s) 188
BtsCI GGATG 4 cut(s) 53, 462, 708, 716
BtsIMutI CAGTG 3 cut(s) 625, 685, 1109
Cac8I GCNNGC 2 cut(s) 157, 862
CaiI CAGNNNCTG 1 cut(s) 1111
CciI TCATGA 1 cut(s) 747
CfoI GCGC 4 cut(s) 86, 161, 293, 561
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 2 cut(s) 149, 321
CseI GACGC 1 cut(s) 226
Csp6I GTAC 2 cut(s) 447, 735
CspCI CAANNNNNGTGG 2 cut(s) 601, 636
CviAII CATG 4 cut(s) 421, 748, 848, 1073
CviQI GTAC 2 cut(s) 447, 735
DdeI CTNAG 7 cut(s) 359, 369, 758, 795, 981, 1056, 1112
DpnI GATC 4 cut(s) 386, 417, 438, 989
DpnII GATC 4 cut(s) 384, 415, 436, 987
EaeI YGGCCR 2 cut(s) 814, 1097
Eam1104I CTCTTC 3 cut(s) 130, 494, 996
EarI CTCTTC 3 cut(s) 130, 494, 996
EciI GGCGGA 2 cut(s) 56, 123
Ecl136II GAGCTC 1 cut(s) 49
Eco130I CCWWGG 2 cut(s) 612, 1100
Eco24I GRGCYC 2 cut(s) 51, 335
Eco47I GGWCC 2 cut(s) 149, 321
Eco53kI GAGCTC 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 1020
EcoICRI GAGCTC 1 cut(s) 49
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoO109I RGGNCCY 1 cut(s) 149
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 3 cut(s) 8, 145, 826
EcoT14I CCWWGG 2 cut(s) 612, 1100
EcoT22I ATGCAT 1 cut(s) 1034
EcoT38I GRGCYC 2 cut(s) 51, 335
ErhI CCWWGG 2 cut(s) 612, 1100
Esp3I CGTCTC 1 cut(s) 96
FaeI CATG 4 cut(s) 424, 751, 851, 1076
FatI CATG 4 cut(s) 420, 747, 847, 1072
FauI CCCGC 1 cut(s) 160
FauNDI CATATG 1 cut(s) 804
FbaI TGATCA 1 cut(s) 384
Fnu4HI GCNGC 7 cut(s) 171, 184, 273, 310, 313, 873, 1106
FokI GGATG 4 cut(s) 40, 449, 703, 715
FriOI GRGCYC 2 cut(s) 51, 335
Fsp4HI GCNGC 7 cut(s) 171, 184, 273, 310, 313, 873, 1106
FspBI CTAG 1 cut(s) 156
GlaI GCGC 4 cut(s) 85, 160, 292, 560
GluI GCNGC 7 cut(s) 171, 184, 273, 310, 313, 873, 1106
HaeII RGCGCY 1 cut(s) 162
HaeIII GGCC 3 cut(s) 170, 816, 1099
HapII CCGG 1 cut(s) 209
HgaI GACGC 1 cut(s) 226
HhaI GCGC 4 cut(s) 86, 161, 293, 561
Hin1II CATG 4 cut(s) 424, 751, 851, 1076
Hin6I GCGC 4 cut(s) 84, 159, 291, 559
HinP1I GCGC 4 cut(s) 84, 159, 291, 559
HincII GTYRAC 1 cut(s) 426
HindII GTYRAC 1 cut(s) 426
HinfI GANTC 5 cut(s) 234, 278, 744, 921, 1060
HpaII CCGG 1 cut(s) 209
HphI GGTGA 2 cut(s) 425, 645
Hpy166II GTNNAC 3 cut(s) 426, 623, 737
Hpy188I TCNGA 9 cut(s) 46, 202, 216, 305, 321, 362, 484, 759, 992
Hpy188III TCNNGA 5 cut(s) 241, 296, 340, 748, 1064
Hpy8I GTNNAC 3 cut(s) 426, 623, 737
Hpy99I CGWCG 6 cut(s) 38, 41, 206, 220, 242, 266
HpyAV CCTTC 7 cut(s) 67, 105, 337, 427, 529, 825, 900
HpyCH4III ACNGT 1 cut(s) 620
HpyCH4IV ACGT 3 cut(s) 39, 695, 948
HpyCH4V TGCA 8 cut(s) 75, 178, 396, 518, 532, 860, 1032, 1127
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 167, 857, 1105
HpyF3I CTNAG 7 cut(s) 359, 369, 758, 795, 981, 1056, 1112
HpySE526I ACGT 3 cut(s) 39, 695, 948
Hsp92II CATG 4 cut(s) 424, 751, 851, 1076
HspAI GCGC 4 cut(s) 84, 159, 291, 559
Ksp22I TGATCA 1 cut(s) 384
Kzo9I GATC 4 cut(s) 384, 415, 436, 987
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 6 cut(s) 54, 191, 291, 338, 676, 1088
Lsp1109I GCAGC 2 cut(s) 859, 1117
LweI GCATC 2 cut(s) 165, 1029
MaeI CTAG 1 cut(s) 156
MaeII ACGT 3 cut(s) 39, 695, 948
MaeIII GTNAC 3 cut(s) 580, 696, 775
MalI GATC 4 cut(s) 386, 417, 438, 989
MbiI CCGCTC 1 cut(s) 186
MboI GATC 4 cut(s) 384, 415, 436, 987
MboII GAAGA 7 cut(s) 26, 117, 269, 511, 956, 1013, 1157
MfeI CAATTG 1 cut(s) 450
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 2 cut(s) 51, 335
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 6 cut(s) 299, 450, 606, 641, 648, 1009
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 1 cut(s) 243
MmeI TCCRAC 2 cut(s) 225, 239
MnlI CCTC 9 cut(s) 130, 133, 247, 299, 478, 565, 731, 976, 997
Mox20I TGGCCA 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 1034
MscI TGGCCA 1 cut(s) 816
MseI TTAA 3 cut(s) 227, 576, 1118
Msp20I TGGCCA 1 cut(s) 816
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 3 cut(s) 10, 147, 828
MunI CAATTG 1 cut(s) 450
MvaI CCWGG 3 cut(s) 10, 147, 828
MvnI CGCG 4 cut(s) 84, 173, 291, 559
MwoI GCNNNNNNNGC 4 cut(s) 81, 167, 857, 1105
NdeI CATATG 1 cut(s) 804
NdeII GATC 4 cut(s) 384, 415, 436, 987
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 4 cut(s) 424, 751, 851, 1076
NlaIV GGNNCC 9 cut(s) 6, 151, 213, 287, 323, 334, 438, 825, 888
NmuCI GTSAC 2 cut(s) 580, 775
NsiI ATGCAT 1 cut(s) 1034
PagI TCATGA 1 cut(s) 747
PasI CCCWGGG 1 cut(s) 9
PciSI GCTCTTC 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PfeI GAWTC 4 cut(s) 278, 744, 921, 1060
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 7 cut(s) 172, 185, 274, 311, 314, 874, 1107
PleI GAGTC 1 cut(s) 242
PpsI GAGTC 1 cut(s) 242
PpuMI RGGWCCY 1 cut(s) 149
Psp124BI GAGCTC 1 cut(s) 51
Psp1406I AACGTT 1 cut(s) 695
Psp5II RGGWCCY 1 cut(s) 149
Psp6I CCWGG 3 cut(s) 8, 145, 826
PspGI CCWGG 3 cut(s) 8, 145, 826
PspN4I GGNNCC 9 cut(s) 6, 151, 213, 287, 323, 334, 438, 825, 888
PspPI GGNCC 2 cut(s) 149, 321
PspPPI RGGWCCY 1 cut(s) 149
PstI CTGCAG 1 cut(s) 534
PstNI CAGNNNCTG 1 cut(s) 1111
PsuI RGATCY 1 cut(s) 436
RsaI GTAC 2 cut(s) 448, 736
RsaNI GTAC 2 cut(s) 447, 735
SacI GAGCTC 1 cut(s) 51
SapI GCTCTTC 1 cut(s) 494
SaqAI TTAA 3 cut(s) 227, 576, 1118
SatI GCNGC 7 cut(s) 171, 184, 273, 310, 313, 873, 1106
Sau3AI GATC 4 cut(s) 384, 415, 436, 987
Sau96I GGNCC 2 cut(s) 149, 321
SchI GAGTC 1 cut(s) 243
ScrFI CCNGG 3 cut(s) 10, 147, 828
SduI GDGCHC 2 cut(s) 51, 335
SfaNI GCATC 2 cut(s) 165, 1029
SfcI CTRYAG 3 cut(s) 441, 530, 876
SinI GGWCC 2 cut(s) 149, 321
Sse9I AATT 6 cut(s) 299, 450, 606, 641, 648, 1009
SspMI CTAG 1 cut(s) 156
SstI GAGCTC 1 cut(s) 51
StyD4I CCNGG 3 cut(s) 8, 145, 826
StyI CCWWGG 2 cut(s) 612, 1100
TaaI ACNGT 1 cut(s) 620
TaiI ACGT 3 cut(s) 42, 698, 951
TaqI TCGA 3 cut(s) 24, 240, 297
TasI AATT 6 cut(s) 299, 450, 606, 641, 648, 1009
TatI WGTACW 1 cut(s) 734
TauI GCSGC 5 cut(s) 173, 186, 275, 312, 315
TfiI GAWTC 4 cut(s) 278, 744, 921, 1060
Tru1I TTAA 3 cut(s) 227, 576, 1118
Tru9I TTAA 3 cut(s) 227, 576, 1118
TscAI CASTG 3 cut(s) 625, 685, 1116
TseFI GTSAC 2 cut(s) 580, 775
TseI GCWGC 2 cut(s) 872, 1105
Tsp45I GTSAC 2 cut(s) 580, 775
TspDTI ATGAA 4 cut(s) 117, 764, 882, 1002
TspGWI ACGGA 2 cut(s) 261, 715
TspRI CASTG 3 cut(s) 625, 685, 1116
VpaK11BI GGWCC 2 cut(s) 149, 321
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 2 cut(s) 299, 641
XcmI CCANNNNNNNNNTGG 2 cut(s) 824, 1034
XspI CTAG 1 cut(s) 156
Zsp2I ATGCAT 1 cut(s) 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.