MD06G1227000.v1.1

Domain of unknown function (DUF4539)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0006732
Physical Location & Seq
Forward (+)
35773941 .. 35775490
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1227000.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGACGACGACGGCAGCGATGAACCCTGGGAGCAACTAGACGTCGACGATTCGGACCTCATCTCTCTCAGTCTCCGCCCTTGCAAGCGCTCCGCCTCCCATTCCCTCAAATCAATTAATCCTCCGCCTCCGCCCAACCCCTCCTCGTCCTCTTCCGCCTCTCCGCCTCTCATTCCGGGCCCCGCCGGAGCCGTACAGGCCGCGATGCAGCGCAGGACCCAAATTCACCGCCACGGAGACGACCAGAACGTCGAGCCCATTCCGACCCAAGAGTTCGTTAGGAGAGTCGTCGAGAATGGTGATGCTAAAGACACCGATTTCGCCGCCGATCCATGGCTTTCCGCTCTCGATTATGTTGCAAAGCAAGGCGCCGACTATCCGACGCCGCTGGGTTCCATCAGGATGGGGGTTAACACTCACAGAGTAGCTCAGGTTGTTGCTGTGATCAAATCTTGCACCCCAAATGGTCTTGGTGATCTCATGCTCACTCTCAAGGTTACGTTCTATGGACATGGATCTCGCGTCAATCTTTTGGTTGGCAAAGATCCTGACATGGTTCAGGAAACGGCTGCCAGCATCCACCACACAGTGCTTTCTGAGGGAGACTTCGGGAAGAGCATATCTGTTGGTGCAGTTTTGGTACTCAAGAAGGTTGCTGTGTTCTCTCCCTCACGCTCGGCATGCTATCTTAACATAACCAAGAACAACGTGGTTCAGGTCATCTCCAAGGATGGTAGACCTGTCGTGACAAACGATCTTCCTATTTCATCAGTCAACAATCCCGCTCCCAGCAGTGGTAAGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4539

Protein Analysis

269

Amino Acids

28.53

Weight (kDa)

5.96

Isoelectric Point (pI)

41.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HROB PF15072 141 - 244 5.6e-20 Homologous recombination OB-fold protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 248
AatII GACGTC 1 cut(s) 45
AccB1I GGYRCC 1 cut(s) 368
AccBSI CCGCTC 2 cut(s) 344, 785
AccI GTMKAC 2 cut(s) 45, 736
AccII CGCG 2 cut(s) 203, 522
AclWI GGATC 3 cut(s) 323, 523, 539
AcsI RAATTY 1 cut(s) 222
AcyI GRCGYC 3 cut(s) 42, 369, 383
AdeI CACNNNGTG 1 cut(s) 589
AfaI GTAC 2 cut(s) 195, 642
AfeI AGCGCT 1 cut(s) 89
AfiI CCNNNNNNNGG 2 cut(s) 333, 794
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 1 cut(s) 428
AluI AGCT 1 cut(s) 428
Alw26I GTCTC 3 cut(s) 77, 231, 597
AlwI GGATC 3 cut(s) 323, 523, 539
Aor51HI AGCGCT 1 cut(s) 89
AoxI GGCC 2 cut(s) 178, 198
ApaI GGGCCC 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 15, 208, 569
ApoI RAATTY 1 cut(s) 222
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AseI ATTAAT 1 cut(s) 117
AspLEI GCGC 3 cut(s) 90, 213, 371
AspS9I GGNCC 4 cut(s) 55, 178, 179, 216
AsuC2I CCSGG 1 cut(s) 177
AsuHPI GGTGA 3 cut(s) 218, 311, 485
AvaII GGWCC 2 cut(s) 55, 216
BaeGI GKGCMC 1 cut(s) 182
BanI GGYRCC 1 cut(s) 368
BanII GRGCYC 2 cut(s) 182, 258
BbvI GCAGC 3 cut(s) 27, 220, 556
BccI CCATC 3 cut(s) 397, 404, 725
BceAI ACGGC 3 cut(s) 28, 176, 582
BcgI CGANNNNNNTGC 2 cut(s) 338, 372
BciT130I CCWGG 1 cut(s) 28
BclI TGATCA 1 cut(s) 444
BcnI CCSGG 1 cut(s) 177
BcoDI GTCTC 3 cut(s) 77, 231, 597
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 2 cut(s) 91, 372
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 6 cut(s) 16, 201, 209, 324, 386, 570
BlsI GCNGC 6 cut(s) 17, 202, 210, 325, 387, 571
Bme1390I CCNGG 2 cut(s) 28, 177
Bme18I GGWCC 2 cut(s) 55, 216
BmgT120I GGNCC 4 cut(s) 55, 178, 179, 216
BmiI GGNNCC 6 cut(s) 180, 181, 190, 218, 370, 394
BmrFI CCNGG 2 cut(s) 28, 177
BmsI GCATC 3 cut(s) 195, 292, 585
Bpu10I CCTNAGC 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 476, 629
BpuMI CCSGG 1 cut(s) 177
BsaHI GRCGYC 3 cut(s) 42, 369, 383
BsaJI CCNNGG 5 cut(s) 26, 27, 232, 332, 726
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 794
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 5 cut(s) 26, 27, 232, 332, 726
BseGI GGATG 3 cut(s) 408, 576, 736
BseLI CCNNNNNNNGG 2 cut(s) 333, 794
BseMII CTCAG 3 cut(s) 82, 443, 588
BseRI GAGGAG 1 cut(s) 133
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 3 cut(s) 27, 220, 556
BseYI CCCAGC 2 cut(s) 388, 788
BsgI GTGCAG 1 cut(s) 651
Bsh1236I CGCG 2 cut(s) 203, 522
BshFI GGCC 2 cut(s) 180, 200
BshNI GGYRCC 1 cut(s) 368
BsiSI CCGG 2 cut(s) 176, 186
BslI CCNNNNNNNGG 2 cut(s) 333, 794
BsmAI GTCTC 3 cut(s) 77, 231, 597
BsmBI CGTCTC 1 cut(s) 231
BsnI GGCC 2 cut(s) 180, 200
Bsp120I GGGCCC 1 cut(s) 178
Bsp1286I GDGCHC 2 cut(s) 182, 258
Bsp143I GATC 6 cut(s) 328, 444, 475, 515, 544, 754
Bsp19I CCATGG 1 cut(s) 332
BspANI GGCC 2 cut(s) 180, 200
BspCNI CTCAG 3 cut(s) 81, 442, 589
BspFNI CGCG 2 cut(s) 203, 522
BspLI GGNNCC 6 cut(s) 180, 181, 190, 218, 370, 394
BspPI GGATC 3 cut(s) 323, 523, 539
BspQI GCTCTTC 1 cut(s) 608
BspT107I GGYRCC 1 cut(s) 368
BsrBI CCGCTC 2 cut(s) 344, 785
BssECI CCNNGG 5 cut(s) 26, 27, 232, 332, 726
BssMI GATC 6 cut(s) 328, 444, 475, 515, 544, 754
BssNI GRCGYC 3 cut(s) 42, 369, 383
BssT1I CCWWGG 2 cut(s) 332, 726
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 1 cut(s) 589
Bst6I CTCTTC 2 cut(s) 157, 608
BstACI GRCGYC 3 cut(s) 42, 369, 383
BstC8I GCNNGC 3 cut(s) 86, 574, 682
BstDEI CTNAG 3 cut(s) 68, 429, 597
BstDSI CCRYGG 2 cut(s) 232, 332
BstF5I GGATG 3 cut(s) 408, 576, 736
BstFNI CGCG 2 cut(s) 203, 522
BstH2I RGCGCY 2 cut(s) 91, 372
BstHHI GCGC 3 cut(s) 90, 213, 371
BstKTI GATC 6 cut(s) 331, 447, 478, 518, 547, 757
BstMAI GTCTC 3 cut(s) 77, 231, 597
BstMBI GATC 6 cut(s) 328, 444, 475, 515, 544, 754
BstMWI GCNNNNNNNGC 2 cut(s) 197, 681
BstNI CCWGG 1 cut(s) 28
BstNSI RCATGY 1 cut(s) 684
BstSCI CCNGG 2 cut(s) 26, 175
BstSLI GKGCMC 1 cut(s) 182
BstUI CGCG 2 cut(s) 203, 522
BstV1I GCAGC 3 cut(s) 27, 220, 556
BstX2I RGATCY 2 cut(s) 515, 544
BstXI CCANNNNNNTGG 1 cut(s) 403
BstYI RGATCY 2 cut(s) 515, 544
BsuRI GGCC 2 cut(s) 180, 200
BtgI CCRYGG 2 cut(s) 232, 332
BtgZI GCGATG 2 cut(s) 33, 218
BtsCI GGATG 3 cut(s) 408, 576, 736
BtsI GCAGTG 1 cut(s) 799
BtsIMutI CAGTG 2 cut(s) 594, 799
Cac8I GCNNGC 3 cut(s) 86, 574, 682
CfoI GCGC 3 cut(s) 90, 213, 371
Cfr13I GGNCC 4 cut(s) 55, 178, 179, 216
CseI GACGC 2 cut(s) 391, 511
Csp6I GTAC 2 cut(s) 194, 641
CviAII CATG 5 cut(s) 333, 481, 512, 553, 681
CviJI RGCY 7 cut(s) 180, 191, 200, 256, 337, 428, 569
CviKI_1 RGCY 7 cut(s) 180, 191, 200, 256, 337, 428, 569
CviQI GTAC 2 cut(s) 194, 641
DdeI CTNAG 3 cut(s) 68, 429, 597
DinI GGCGCC 1 cut(s) 370
DpnI GATC 6 cut(s) 330, 446, 477, 517, 546, 756
DpnII GATC 6 cut(s) 328, 444, 475, 515, 544, 754
DraIII CACNNNGTG 1 cut(s) 589
DrdI GACNNNNNNGTC 1 cut(s) 248
DseDI GACNNNNNNGTC 1 cut(s) 248
Eam1104I CTCTTC 2 cut(s) 157, 608
EarI CTCTTC 2 cut(s) 157, 608
EciI GGCGGA 6 cut(s) 65, 82, 114, 120, 145, 153
Eco130I CCWWGG 2 cut(s) 332, 726
Eco24I GRGCYC 2 cut(s) 182, 258
Eco47I GGWCC 2 cut(s) 55, 216
Eco47III AGCGCT 1 cut(s) 89
EcoO109I RGGNCCY 2 cut(s) 179, 216
EcoRII CCWGG 1 cut(s) 26
EcoT14I CCWWGG 2 cut(s) 332, 726
EcoT38I GRGCYC 2 cut(s) 182, 258
EgeI GGCGCC 1 cut(s) 370
EheI GGCGCC 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 332, 726
Esp3I CGTCTC 1 cut(s) 231
FaeI CATG 5 cut(s) 336, 484, 515, 556, 684
FatI CATG 5 cut(s) 332, 480, 511, 552, 680
FauI CCCGC 2 cut(s) 190, 790
FbaI TGATCA 1 cut(s) 444
FblI GTMKAC 2 cut(s) 45, 736
Fnu4HI GCNGC 6 cut(s) 16, 201, 209, 324, 386, 570
FokI GGATG 3 cut(s) 415, 563, 743
FriOI GRGCYC 2 cut(s) 182, 258
Fsp4HI GCNGC 6 cut(s) 16, 201, 209, 324, 386, 570
FspBI CTAG 1 cut(s) 38
GlaI GCGC 3 cut(s) 89, 212, 370
GluI GCNGC 6 cut(s) 16, 201, 209, 324, 386, 570
GsaI CCCAGC 2 cut(s) 392, 792
HaeII RGCGCY 2 cut(s) 91, 372
HaeIII GGCC 2 cut(s) 180, 200
HapII CCGG 2 cut(s) 176, 186
HgaI GACGC 2 cut(s) 391, 511
HhaI GCGC 3 cut(s) 90, 213, 371
Hin1I GRCGYC 3 cut(s) 42, 369, 383
Hin1II CATG 5 cut(s) 336, 484, 515, 556, 684
Hin6I GCGC 3 cut(s) 88, 211, 369
HinP1I GCGC 3 cut(s) 88, 211, 369
HincII GTYRAC 3 cut(s) 46, 412, 775
HindII GTYRAC 3 cut(s) 46, 412, 775
HinfI GANTC 2 cut(s) 50, 285
HpaI GTTAAC 1 cut(s) 412
HpaII CCGG 2 cut(s) 176, 186
HphI GGTGA 3 cut(s) 218, 311, 485
Hpy166II GTNNAC 4 cut(s) 46, 412, 737, 775
Hpy188I TCNGA 4 cut(s) 55, 264, 381, 598
Hpy188III TCNNGA 8 cut(s) 292, 347, 400, 548, 560, 610, 646, 745
Hpy8I GTNNAC 4 cut(s) 46, 412, 737, 775
Hpy99I CGWCG 7 cut(s) 11, 14, 47, 50, 254, 293, 385
HpyAV CCTTC 1 cut(s) 643
HpyCH4III ACNGT 1 cut(s) 589
HpyCH4IV ACGT 4 cut(s) 42, 249, 500, 708
HpyCH4V TGCA 5 cut(s) 84, 208, 359, 456, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 681
HpyF3I CTNAG 3 cut(s) 68, 429, 597
HpySE526I ACGT 4 cut(s) 42, 249, 500, 708
Hsp92I GRCGYC 3 cut(s) 42, 369, 383
Hsp92II CATG 5 cut(s) 336, 484, 515, 556, 684
HspAI GCGC 3 cut(s) 88, 211, 369
KasI GGCGCC 1 cut(s) 368
Ksp22I TGATCA 1 cut(s) 444
KspAI GTTAAC 1 cut(s) 412
Kzo9I GATC 6 cut(s) 328, 444, 475, 515, 544, 754
LguI GCTCTTC 1 cut(s) 608
LmnI GCTCC 4 cut(s) 31, 95, 188, 790
Lsp1109I GCAGC 3 cut(s) 27, 220, 556
LweI GCATC 3 cut(s) 195, 292, 585
MaeI CTAG 1 cut(s) 38
MaeII ACGT 4 cut(s) 42, 249, 500, 708
MaeIII GTNAC 2 cut(s) 496, 745
MalI GATC 6 cut(s) 330, 446, 477, 517, 546, 756
MbiI CCGCTC 2 cut(s) 344, 785
MboI GATC 6 cut(s) 328, 444, 475, 515, 544, 754
MboII GAAGA 3 cut(s) 144, 625, 749
MflI RGATCY 2 cut(s) 515, 544
MhlI GDGCHC 2 cut(s) 182, 258
MluCI AATT 2 cut(s) 114, 222
Mly113I GGCGCC 1 cut(s) 369
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 2 cut(s) 287, 404
MseI TTAA 3 cut(s) 117, 411, 690
MslI CAYNNNNRTG 1 cut(s) 401
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 2 cut(s) 176, 186
MspR9I CCNGG 2 cut(s) 28, 177
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 2 cut(s) 203, 522
MwoI GCNNNNNNNGC 2 cut(s) 197, 681
NarI GGCGCC 1 cut(s) 369
NciI CCSGG 1 cut(s) 177
NcoI CCATGG 1 cut(s) 332
NdeII GATC 6 cut(s) 328, 444, 475, 515, 544, 754
NlaIII CATG 5 cut(s) 336, 484, 515, 556, 684
NlaIV GGNNCC 6 cut(s) 180, 181, 190, 218, 370, 394
NmeAIII GCCGAG 1 cut(s) 656
NmuCI GTSAC 1 cut(s) 745
NspI RCATGY 1 cut(s) 684
PaeI GCATGC 1 cut(s) 684
PasI CCCWGGG 1 cut(s) 27
PciSI GCTCTTC 1 cut(s) 608
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 246, 750
PfeI GAWTC 1 cut(s) 50
PkrI GCNGC 6 cut(s) 17, 202, 210, 325, 387, 571
PleI GAGTC 1 cut(s) 293
PluTI GGCGCC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 216
PshBI ATTAAT 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 26
PspFI CCCAGC 2 cut(s) 388, 788
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 6 cut(s) 180, 181, 190, 218, 370, 394
PspOMI GGGCCC 1 cut(s) 178
PspPI GGNCC 4 cut(s) 55, 178, 179, 216
PspPPI RGGWCCY 1 cut(s) 216
PsuI RGATCY 2 cut(s) 515, 544
RsaI GTAC 2 cut(s) 195, 642
RsaNI GTAC 2 cut(s) 194, 641
RseI CAYNNNNRTG 1 cut(s) 401
SalI GTCGAC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 608
SaqAI TTAA 3 cut(s) 117, 411, 690
SatI GCNGC 6 cut(s) 16, 201, 209, 324, 386, 570
Sau3AI GATC 6 cut(s) 328, 444, 475, 515, 544, 754
Sau96I GGNCC 4 cut(s) 55, 178, 179, 216
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 2 cut(s) 28, 177
SduI GDGCHC 2 cut(s) 182, 258
SfaNI GCATC 3 cut(s) 195, 292, 585
SfoI GGCGCC 1 cut(s) 370
SgrDI CGTCGACG 1 cut(s) 44
SinI GGWCC 2 cut(s) 55, 216
SmiMI CAYNNNNRTG 1 cut(s) 401
SmlI CTYRAG 2 cut(s) 491, 644
SmoI CTYRAG 2 cut(s) 491, 644
SphI GCATGC 1 cut(s) 684
Sse9I AATT 2 cut(s) 114, 222
SspDI GGCGCC 1 cut(s) 368
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 2 cut(s) 26, 175
StyI CCWWGG 2 cut(s) 332, 726
TaaI ACNGT 1 cut(s) 589
TaiI ACGT 4 cut(s) 45, 252, 503, 711
TaqI TCGA 4 cut(s) 45, 252, 291, 348
TasI AATT 2 cut(s) 114, 222
TauI GCSGC 3 cut(s) 203, 326, 388
TfiI GAWTC 1 cut(s) 50
Tru1I TTAA 3 cut(s) 117, 411, 690
Tru9I TTAA 3 cut(s) 117, 411, 690
TscAI CASTG 2 cut(s) 594, 799
TseFI GTSAC 1 cut(s) 745
TseI GCWGC 3 cut(s) 15, 208, 569
Tsp45I GTSAC 1 cut(s) 745
TspDTI ATGAA 2 cut(s) 36, 756
TspGWI ACGGA 1 cut(s) 249
TspRI CASTG 2 cut(s) 594, 799
VpaK11BI GGWCC 2 cut(s) 55, 216
VspI ATTAAT 1 cut(s) 117
XapI RAATTY 1 cut(s) 222
XceI RCATGY 1 cut(s) 684
XcmI CCANNNNNNNNNTGG 1 cut(s) 706
XmiI GTMKAC 2 cut(s) 45, 736
XspI CTAG 1 cut(s) 38
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.