AT1G49770

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006234
Physical Location & Seq
Forward (+)
18424578 .. 18426782
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G49770.1

Sequence Viewer

Length: 927 bp
ATGACTAATGCTCAAGAGTTGGGGCAAGAGGGTTTTATGTGGGGCATATCCAATTCTGATGATTCCGGAGGTGGTTGCAAAAGAATCGAGAAAGAACCGCTTCCATCACATCCGTCTCATCCGTCGCCAGAGATCCAGACTACGACGGTTAAAAAGGGAAAAAAGAGAACGAAAAGAAATGATAAAAACCATGAAGAAGAATCACCTGATCATGAAATACATATATGGACCGAAAGAGAAAGGAGAAAGAAGATGAGGGACATGTTTTCTAAATTACATGCTTTGCTTCCCCAACTTCCTCCTAAGGCAGACAAATCAACAATTGTAGATGAAGCAGTGAGTTCAATCAAATCCCTTGAACAAACTTTGCAAAAACTTGAAATGCAAAAGCTTGAGAAGCTTCAGTATTCTTCTGCCTCAACAAACACAACTCCTACTACAACTTTTGCTTATGCTCCATCATCATCCTCTTCTCCAACAGCTCTCCTCACACCAATATCAAACCATCCCATAGACGCCACCGCAACAGATTCTTACCCTCGCGCAGCTTTCTTGGCCGATCAGGTATCTTCTTCCAGTGCCGCTGCTGCGAACCTGCCTTACCCTTGTAATGATCCGATCGTGAATTTCGATACTTGGTCCTCACGTAACGTGGTGCTGACTATCTGTGGGAATGAAGCTTTCTTCAATTTGTGTGTCCCTAAACACAAACCAGGGGTTTTCACTTCTGTTTGTTACTTGTTTGAGAAGTACAACATGGAGGTTTTGTTTGCTAATGTCTCCTCTAATGTTTTCTGGAGCACCTACGTGATCCAAGCACAGGTAAATCCGAGTTGTGAGAATCAGTTACTGGGAAATGGTCTTGGAGTTGTAGACGTTTTCAAGCAAGTTTCTCAAGAACTGGTGTTATATTTTTCATCTCTATAA

Protein Analysis

308

Amino Acids

34.18

Weight (kDa)

6.25

Isoelectric Point (pI)

45.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 74 - 120 5.5e-09 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 603
AccI GTMKAC 1 cut(s) 873
AccII CGCG 1 cut(s) 543
AccIII TCCGGA 1 cut(s) 65
AciI CCGC 3 cut(s) 98, 522, 582
AclWI GGATC 3 cut(s) 127, 608, 805
AcoI YGGCCR 1 cut(s) 555
AcsI RAATTY 1 cut(s) 625
AcuI CTGAAG 1 cut(s) 386
AcyI GRCGYC 1 cut(s) 516
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 1 cut(s) 820
AflIII ACRYGT 1 cut(s) 261
AgsI TTSAA 5 cut(s) 345, 359, 380, 688, 883
AjnI CCWGG 1 cut(s) 712
AleI CACNNNNGTG 1 cut(s) 806
AluBI AGCT 5 cut(s) 391, 400, 482, 548, 680
AluI AGCT 5 cut(s) 391, 400, 482, 548, 680
Alw21I GWGCWC 1 cut(s) 803
Alw26I GTCTC 2 cut(s) 120, 784
AlwI GGATC 3 cut(s) 127, 608, 805
AlwNI CAGNNNCTG 1 cut(s) 850
Aor13HI TCCGGA 1 cut(s) 65
AoxI GGCC 1 cut(s) 555
ApeKI GCWGC 3 cut(s) 545, 584, 587
ApoI RAATTY 1 cut(s) 625
Asp700I GAANNNNTTC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 545
AspS9I GGNCC 2 cut(s) 228, 639
AsuHPI GGTGA 1 cut(s) 195
AvaII GGWCC 2 cut(s) 228, 639
AxyI CCTNAGG 1 cut(s) 303
Bbv12I GWGCWC 1 cut(s) 803
BbvI GCAGC 3 cut(s) 557, 571, 574
BccI CCATC 3 cut(s) 112, 466, 513
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 1 cut(s) 714
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 120, 784
BfuAI ACCTGC 1 cut(s) 603
BisI GCNGC 4 cut(s) 546, 582, 585, 588
BlsI GCNGC 4 cut(s) 547, 583, 586, 589
Bme1390I CCNGG 1 cut(s) 714
Bme18I GGWCC 2 cut(s) 228, 639
BmgT120I GGNCC 2 cut(s) 228, 639
BmrFI CCNGG 1 cut(s) 714
BmrI ACTGGG 1 cut(s) 860
BmuI ACTGGG 1 cut(s) 860
BpmI CTGGAG 1 cut(s) 817
BpuEI CTTGAG 2 cut(s) 413, 879
BsaAI YACGTR 2 cut(s) 647, 808
BsaHI GRCGYC 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 713
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 752, 782
Bsc4I CCNNNNNNNGG 1 cut(s) 820
Bse1I ACTGG 3 cut(s) 576, 855, 906
Bse21I CCTNAGG 1 cut(s) 303
BseAI TCCGGA 1 cut(s) 65
BseBI CCWGG 1 cut(s) 714
BseDI CCNNGG 1 cut(s) 713
BseGI GGATG 4 cut(s) 109, 118, 464, 505
BseLI CCNNNNNNNGG 1 cut(s) 820
BseNI ACTGG 3 cut(s) 576, 855, 906
BseRI GAGGAG 2 cut(s) 476, 772
BseXI GCAGC 3 cut(s) 557, 571, 574
Bsh1236I CGCG 1 cut(s) 543
Bsh1285I CGRYCG 1 cut(s) 621
BshFI GGCC 1 cut(s) 557
BsiEI CGRYCG 1 cut(s) 621
BsiHKAI GWGCWC 1 cut(s) 803
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 2 cut(s) 272, 683
BslI CCNNNNNNNGG 1 cut(s) 820
BsmAI GTCTC 2 cut(s) 120, 784
BsmBI CGTCTC 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 272, 683
BsnI GGCC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 803
Bsp13I TCCGGA 1 cut(s) 65
Bsp143I GATC 6 cut(s) 132, 208, 559, 613, 618, 810
BspACI CCGC 3 cut(s) 98, 522, 582
BspANI GGCC 1 cut(s) 557
BspEI TCCGGA 1 cut(s) 65
BspFNI CGCG 1 cut(s) 543
BspHI TCATGA 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 603
BspPI GGATC 3 cut(s) 127, 608, 805
BsrI ACTGG 3 cut(s) 576, 855, 906
BssECI CCNNGG 1 cut(s) 713
BssMI GATC 6 cut(s) 132, 208, 559, 613, 618, 810
BssNI GRCGYC 1 cut(s) 516
Bst2UI CCWGG 1 cut(s) 714
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 475
BstACI GRCGYC 1 cut(s) 516
BstBAI YACGTR 2 cut(s) 647, 808
BstDEI CTNAG 1 cut(s) 303
BstF5I GGATG 4 cut(s) 109, 118, 464, 505
BstFNI CGCG 1 cut(s) 543
BstHHI GCGC 1 cut(s) 545
BstKTI GATC 6 cut(s) 135, 211, 562, 616, 621, 813
BstMAI GTCTC 2 cut(s) 120, 784
BstMBI GATC 6 cut(s) 132, 208, 559, 613, 618, 810
BstMCI CGRYCG 1 cut(s) 621
BstMWI GCNNNNNNNGC 3 cut(s) 397, 554, 587
BstNI CCWGG 1 cut(s) 714
BstNSI RCATGY 2 cut(s) 265, 281
BstSCI CCNGG 1 cut(s) 712
BstUI CGCG 1 cut(s) 543
BstV1I GCAGC 3 cut(s) 557, 571, 574
BstX2I RGATCY 1 cut(s) 132
BstYI RGATCY 1 cut(s) 132
Bsu36I CCTNAGG 1 cut(s) 303
BsuRI GGCC 1 cut(s) 557
BtsCI GGATG 4 cut(s) 109, 118, 464, 505
BtsI GCAGTG 1 cut(s) 342
BtsIMutI CAGTG 2 cut(s) 342, 583
BveI ACCTGC 1 cut(s) 603
CaiI CAGNNNCTG 1 cut(s) 850
CciI TCATGA 1 cut(s) 211
CfoI GCGC 1 cut(s) 545
Cfr13I GGNCC 2 cut(s) 228, 639
CseI GACGC 1 cut(s) 524
Csp6I GTAC 1 cut(s) 751
CviAII CATG 5 cut(s) 191, 212, 262, 278, 757
CviJI RGCY 6 cut(s) 391, 400, 482, 548, 557, 680
CviKI_1 RGCY 6 cut(s) 391, 400, 482, 548, 557, 680
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 1 cut(s) 303
DpnI GATC 6 cut(s) 134, 210, 561, 615, 620, 812
DpnII GATC 6 cut(s) 132, 208, 559, 613, 618, 810
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 228, 639
Eco57I CTGAAG 1 cut(s) 386
Eco81I CCTNAGG 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 712
Esp3I CGTCTC 1 cut(s) 120
FaeI CATG 5 cut(s) 194, 215, 265, 281, 760
FalI AAGNNNNNCTT 2 cut(s) 84, 116
FaqI GGGAC 2 cut(s) 272, 683
FatI CATG 5 cut(s) 190, 211, 261, 277, 756
FbaI TGATCA 1 cut(s) 208
FblI GTMKAC 1 cut(s) 873
Fnu4HI GCNGC 4 cut(s) 546, 582, 585, 588
FokI GGATG 4 cut(s) 96, 105, 451, 492
Fsp4HI GCNGC 4 cut(s) 546, 582, 585, 588
GlaI GCGC 1 cut(s) 544
GluI GCNGC 4 cut(s) 546, 582, 585, 588
GsuI CTGGAG 1 cut(s) 817
HaeIII GGCC 1 cut(s) 557
HapII CCGG 1 cut(s) 66
HgaI GACGC 1 cut(s) 524
HhaI GCGC 1 cut(s) 545
Hin1I GRCGYC 1 cut(s) 516
Hin1II CATG 5 cut(s) 194, 215, 265, 281, 760
Hin6I GCGC 1 cut(s) 543
HinP1I GCGC 1 cut(s) 543
HindIII AAGCTT 3 cut(s) 389, 398, 678
HinfI GANTC 5 cut(s) 62, 84, 200, 530, 841
HpaII CCGG 1 cut(s) 66
HphI GGTGA 1 cut(s) 195
Hpy166II GTNNAC 1 cut(s) 874
Hpy188I TCNGA 3 cut(s) 58, 618, 831
Hpy188III TCNNGA 8 cut(s) 14, 66, 88, 136, 212, 622, 796, 896
Hpy8I GTNNAC 1 cut(s) 874
Hpy99I CGWCG 2 cut(s) 127, 148
HpyCH4III ACNGT 1 cut(s) 148
HpyCH4IV ACGT 4 cut(s) 646, 651, 807, 876
HpyCH4V TGCA 3 cut(s) 78, 370, 385
HpyF10VI GCNNNNNNNGC 3 cut(s) 397, 554, 587
HpyF3I CTNAG 1 cut(s) 303
HpySE526I ACGT 4 cut(s) 646, 651, 807, 876
Hsp92I GRCGYC 1 cut(s) 516
Hsp92II CATG 5 cut(s) 194, 215, 265, 281, 760
HspAI GCGC 1 cut(s) 543
Kpn2I TCCGGA 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 6 cut(s) 132, 208, 559, 613, 618, 810
LmnI GCTCC 2 cut(s) 460, 798
Lsp1109I GCAGC 3 cut(s) 557, 571, 574
MaeII ACGT 4 cut(s) 646, 651, 807, 876
MaeIII GTNAC 3 cut(s) 647, 734, 846
MalI GATC 6 cut(s) 134, 210, 561, 615, 620, 812
MboI GATC 6 cut(s) 132, 208, 559, 613, 618, 810
MboII GAAGA 8 cut(s) 206, 209, 262, 402, 462, 561, 564, 676
MfeI CAATTG 1 cut(s) 321
MflI RGATCY 1 cut(s) 132
MhlI GDGCHC 1 cut(s) 803
MluCI AATT 5 cut(s) 52, 272, 321, 625, 688
MmeI TCCRAC 1 cut(s) 500
MroI TCCGGA 1 cut(s) 65
MroXI GAANNNNTTC 1 cut(s) 99
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 1 cut(s) 806
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 1 cut(s) 66
MspR9I CCNGG 1 cut(s) 714
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 1 cut(s) 714
MvnI CGCG 1 cut(s) 543
MwoI GCNNNNNNNGC 3 cut(s) 397, 554, 587
NdeII GATC 6 cut(s) 132, 208, 559, 613, 618, 810
NlaIII CATG 5 cut(s) 194, 215, 265, 281, 760
NspI RCATGY 2 cut(s) 265, 281
OliI CACNNNNGTG 1 cut(s) 806
PagI TCATGA 1 cut(s) 211
PciI ACATGT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 99
PfeI GAWTC 5 cut(s) 62, 84, 200, 530, 841
PkrI GCNGC 4 cut(s) 547, 583, 586, 589
Ple19I CGATCG 1 cut(s) 621
Ppu21I YACGTR 2 cut(s) 647, 808
PscI ACATGT 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 712
PspGI CCWGG 1 cut(s) 712
PspPI GGNCC 2 cut(s) 228, 639
PsrI GAACNNNNNNTAC 2 cut(s) 584, 616
PstNI CAGNNNCTG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 132
PvuI CGATCG 1 cut(s) 621
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
RseI CAYNNNNRTG 1 cut(s) 806
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 4 cut(s) 546, 582, 585, 588
Sau3AI GATC 6 cut(s) 132, 208, 559, 613, 618, 810
Sau96I GGNCC 2 cut(s) 228, 639
ScrFI CCNGG 1 cut(s) 714
SduI GDGCHC 1 cut(s) 803
SinI GGWCC 2 cut(s) 228, 639
SmiMI CAYNNNNRTG 1 cut(s) 806
SmlI CTYRAG 3 cut(s) 12, 392, 894
SmoI CTYRAG 3 cut(s) 12, 392, 894
Sse9I AATT 5 cut(s) 52, 272, 321, 625, 688
SsiI CCGC 3 cut(s) 98, 522, 582
StyD4I CCNGG 1 cut(s) 712
TaaI ACNGT 1 cut(s) 148
TaiI ACGT 4 cut(s) 649, 654, 810, 879
TaqI TCGA 2 cut(s) 87, 630
TaqII GACCGA 1 cut(s) 245
TasI AATT 5 cut(s) 52, 272, 321, 625, 688
TatI WGTACW 1 cut(s) 750
TauI GCSGC 1 cut(s) 584
TfiI GAWTC 5 cut(s) 62, 84, 200, 530, 841
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TscAI CASTG 2 cut(s) 342, 583
TseI GCWGC 3 cut(s) 545, 584, 587
TspDTI ATGAA 5 cut(s) 207, 228, 345, 690, 906
TspGWI ACGGA 2 cut(s) 102, 111
TspRI CASTG 2 cut(s) 342, 583
VpaK11BI GGWCC 2 cut(s) 228, 639
XapI RAATTY 1 cut(s) 625
XceI RCATGY 2 cut(s) 265, 281
XmiI GTMKAC 1 cut(s) 873
XmnI GAANNNNTTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.