FvH4_2g05810
BHLH Family

Transcription factor bHLH95-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0006234
Physical Location & Seq
Forward (+)
4769031 .. 4772685
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05810.t1

Sequence Viewer

Length: 1098 bp
ATGTTTAAACCAGATTCAGGAGCAGGAGCTTCAAATGGGGTTTTATGGGCAAACCAGTCGTGGGCTAATTTGTCCAATTCTACTGATAAATTTTTGTTGAACTCCAATTCTGAGAATTCAGCTGGTGGTGGCGATGAGGGGAAGGATAAATTAGAGAAGGAGAAAGAAATTATAGATAGAGAAGATCAAGTGCAACCAGAGTTAGAGCCGGAGGCTGCTCTCGGTAAGAAAACTTTGGCTAAGAAGCGAGGTGGCCGAGTTAGTGGTAGCAGTTTCAAGAGGTATAACGGGAAGGCTATCAAGGAAAAGGGAAAAGCGAGTAGTGAAGAGGTGGTGAAAGAAGGAAGAAGGGGCAGTGATTCTGAGGATGATCATGAGTTGCATATATACACCGAAAGGGAAAGGAGGAAGAAGATGAGGAACATGTTTGCCAACCTTCATGCTTTGCTTCCTCAACTTCCTTCTAAGGCTGACAAATCCACAATTGTTGATGAAGCGGTCACCTACATAAAAAGGCTTGAAATCACTCTTCAATCCCTACAACAACAAAAGAGGGAGAGGATGCGAAGCCTCCCAAATAGCACTATGGTCAATTACAACCACACACCAATACTTATGAACTCACAAAATGTGGTCTATGACTCAAGAGAGGGTATCCTACCTTCACATGTCTCTTCCAGTAACTTGGTCGATGCTTCTACTACTACTACTACTACTGCAAACGGATTCAACATCATTCCTGATTTCAATCCTTTGAACAATTCATTCTCAACCAACTCACTTTCTCCCGAACCTGTAGTGTTTAAAACATGGACTTCACCTAATGTCGTGTTGAACATGTGCCGAAATGAAGCACAAATTAGTGTGTGCACCCCAAAGAAGCCTGGCATATTTTCCCAGATTTGCTACATTTTGGAGAATTACAAGCTTGTAATGGTAACAGCTCATATTAACTCAGACTCTCATCGCAGTATGTACATGATCCAAGCACAGCCAAGTAGCGCTTCTGATAATCAGCTTCTGGACATGTTTCCAGCAGAGGAAATATTCAAGCAAGCTGTGACAGAGATAAATTTGTGTGTCGTCTCCAGCTTTTAA

Protein Analysis

366

Amino Acids

40.67

Weight (kDa)

7.06

Isoelectric Point (pI)

48.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 128 - 174 2.1e-10 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 497
AclWI GGATC 1 cut(s) 976
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 3 cut(s) 89, 115, 1072
AfaI GTAC 1 cut(s) 977
AfeI AGCGCT 1 cut(s) 1003
AfiI CCNNNNNNNGG 2 cut(s) 17, 61
AflIII ACRYGT 4 cut(s) 423, 667, 837, 1026
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 2 cut(s) 988, 1020
AluBI AGCT 7 cut(s) 29, 122, 928, 944, 1018, 1058, 1092
AluI AGCT 7 cut(s) 29, 122, 928, 944, 1018, 1058, 1092
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 2 cut(s) 676, 1090
Alw44I GTGCAC 1 cut(s) 868
AlwI GGATC 1 cut(s) 976
AlwNI CAGNNNCTG 1 cut(s) 1021
Aor51HI AGCGCT 1 cut(s) 1003
AoxI GGCC 1 cut(s) 253
ApaLI GTGCAC 1 cut(s) 868
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 3 cut(s) 89, 115, 1072
AspLEI GCGC 1 cut(s) 1004
AsuHPI GGTGA 3 cut(s) 346, 493, 810
BaeGI GKGCMC 1 cut(s) 872
BarI GAAGNNNNNNTAC 2 cut(s) 646, 678
Bbv12I GWGCWC 1 cut(s) 872
BbvI GCAGC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 39, 73
BciT130I CCWGG 1 cut(s) 885
BciVI GTATCC 1 cut(s) 665
BclI TGATCA 1 cut(s) 370
BcoDI GTCTC 2 cut(s) 676, 1090
BfmI CTRYAG 1 cut(s) 795
BfoI RGCGCY 1 cut(s) 1005
BfuI GTATCC 1 cut(s) 665
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 885
BmrFI CCNGG 1 cut(s) 885
BmsI GCATC 2 cut(s) 552, 682
BpmI CTGGAG 1 cut(s) 1072
BpuEI CTTGAG 1 cut(s) 628
BsaBI GATNNNNATC 1 cut(s) 747
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 61
Bse1I ACTGG 2 cut(s) 55, 678
Bse8I GATNNNNATC 1 cut(s) 747
BseBI CCWGG 1 cut(s) 885
BseGI GGATG 2 cut(s) 373, 567
BseJI GATNNNNATC 1 cut(s) 747
BseLI CCNNNNNNNGG 2 cut(s) 17, 61
BseMII CTCAG 3 cut(s) 102, 354, 969
BseNI ACTGG 2 cut(s) 55, 678
BseSI GKGCMC 1 cut(s) 872
BseXI GCAGC 1 cut(s) 202
BshFI GGCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 1 cut(s) 209
BslI CCNNNNNNNGG 2 cut(s) 17, 61
BsmAI GTCTC 2 cut(s) 676, 1090
BsmBI CGTCTC 1 cut(s) 1090
BsnI GGCC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 872
Bsp1407I TGTACA 1 cut(s) 975
Bsp143I GATC 3 cut(s) 184, 370, 981
BspACI CCGC 1 cut(s) 497
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 3 cut(s) 103, 355, 968
BspHI TCATGA 1 cut(s) 373
BspPI GGATC 1 cut(s) 976
BsrGI TGTACA 1 cut(s) 975
BsrI ACTGG 2 cut(s) 55, 678
BssMI GATC 3 cut(s) 184, 370, 981
Bst2UI CCWGG 1 cut(s) 885
Bst6I CTCTTC 3 cut(s) 321, 534, 679
BstAUI TGTACA 1 cut(s) 975
BstC8I GCNNGC 1 cut(s) 1056
BstDEI CTNAG 5 cut(s) 111, 240, 363, 465, 955
BstEII GGTNACC 1 cut(s) 499
BstF5I GGATG 2 cut(s) 373, 567
BstH2I RGCGCY 1 cut(s) 1005
BstHHI GCGC 1 cut(s) 1004
BstKTI GATC 3 cut(s) 187, 373, 984
BstMAI GTCTC 2 cut(s) 676, 1090
BstMBI GATC 3 cut(s) 184, 370, 981
BstNI CCWGG 1 cut(s) 885
BstNSI RCATGY 4 cut(s) 427, 671, 841, 1030
BstPI GGTNACC 1 cut(s) 499
BstSCI CCNGG 1 cut(s) 883
BstSFI CTRYAG 1 cut(s) 795
BstSLI GKGCMC 1 cut(s) 872
BstV1I GCAGC 1 cut(s) 202
BstXI CCANNNNNNTGG 1 cut(s) 685
BsuI GTATCC 1 cut(s) 665
BsuRI GGCC 1 cut(s) 255
BtgZI GCGATG 2 cut(s) 147, 950
BtsCI GGATG 2 cut(s) 373, 567
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 1056
CaiI CAGNNNCTG 1 cut(s) 1021
CciI TCATGA 1 cut(s) 373
CfoI GCGC 1 cut(s) 1004
Csp6I GTAC 1 cut(s) 976
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 8 cut(s) 374, 424, 440, 668, 810, 838, 979, 1027
CviQI GTAC 1 cut(s) 976
DdeI CTNAG 5 cut(s) 111, 240, 363, 465, 955
DpnI GATC 3 cut(s) 186, 372, 983
DpnII GATC 3 cut(s) 184, 370, 981
DraI TTTAAA 2 cut(s) 7, 805
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 3 cut(s) 321, 534, 679
EarI CTCTTC 3 cut(s) 321, 534, 679
Eco47III AGCGCT 1 cut(s) 1003
Eco91I GGTNACC 1 cut(s) 499
EcoO65I GGTNACC 1 cut(s) 499
EcoRI GAATTC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 883
Esp3I CGTCTC 1 cut(s) 1090
FaeI CATG 8 cut(s) 377, 427, 443, 671, 813, 841, 982, 1030
FalI AAGNNNNNCTT 2 cut(s) 988, 1020
FatI CATG 8 cut(s) 373, 423, 439, 667, 809, 837, 978, 1026
FbaI TGATCA 1 cut(s) 370
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 2 cut(s) 380, 574
Fsp4HI GCNGC 1 cut(s) 216
GlaI GCGC 1 cut(s) 1003
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 1072
HaeII RGCGCY 1 cut(s) 1005
HaeIII GGCC 1 cut(s) 255
HapII CCGG 1 cut(s) 209
HhaI GCGC 1 cut(s) 1004
Hin1II CATG 8 cut(s) 377, 427, 443, 671, 813, 841, 982, 1030
Hin6I GCGC 1 cut(s) 1002
HinP1I GCGC 1 cut(s) 1002
HindIII AAGCTT 1 cut(s) 926
HinfI GANTC 5 cut(s) 14, 359, 641, 726, 959
HpaII CCGG 1 cut(s) 209
HphI GGTGA 3 cut(s) 346, 493, 810
Hpy166II GTNNAC 1 cut(s) 870
Hpy188I TCNGA 4 cut(s) 112, 364, 958, 1009
Hpy188III TCNNGA 7 cut(s) 18, 277, 374, 645, 740, 788, 1022
Hpy8I GTNNAC 1 cut(s) 870
HpyAV CCTTC 8 cut(s) 136, 151, 286, 335, 342, 446, 471, 672
HpyCH4V TGCA 4 cut(s) 193, 382, 719, 870
HpyF3I CTNAG 5 cut(s) 111, 240, 363, 465, 955
Hsp92II CATG 8 cut(s) 377, 427, 443, 671, 813, 841, 982, 1030
HspAI GCGC 1 cut(s) 1002
Ksp22I TGATCA 1 cut(s) 370
Kzo9I GATC 3 cut(s) 184, 370, 981
LmnI GCTCC 2 cut(s) 20, 26
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 2 cut(s) 552, 682
MaeIII GTNAC 4 cut(s) 499, 680, 937, 1060
MalI GATC 3 cut(s) 186, 372, 983
MboI GATC 3 cut(s) 184, 370, 981
MboII GAAGA 7 cut(s) 194, 338, 357, 421, 424, 521, 666
MfeI CAATTG 1 cut(s) 483
MhlI GDGCHC 1 cut(s) 872
MlyI GAGTC 2 cut(s) 635, 953
MseI TTAA 4 cut(s) 6, 804, 951, 1096
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 1 cut(s) 885
MssI GTTTAAAC 1 cut(s) 7
MunI CAATTG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 885
NdeII GATC 3 cut(s) 184, 370, 981
NlaIII CATG 8 cut(s) 377, 427, 443, 671, 813, 841, 982, 1030
NmeAIII GCCGAG 1 cut(s) 281
NmuCI GTSAC 2 cut(s) 499, 1060
NspI RCATGY 4 cut(s) 427, 671, 841, 1030
PagI TCATGA 1 cut(s) 373
PciI ACATGT 4 cut(s) 423, 667, 837, 1026
PfeI GAWTC 3 cut(s) 14, 359, 726
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 2 cut(s) 635, 953
PmeI GTTTAAAC 1 cut(s) 7
PpsI GAGTC 2 cut(s) 635, 953
PscI ACATGT 4 cut(s) 423, 667, 837, 1026
Psp6I CCWGG 1 cut(s) 883
PspEI GGTNACC 1 cut(s) 499
PspGI CCWGG 1 cut(s) 883
PstNI CAGNNNCTG 1 cut(s) 1021
PvuII CAGCTG 1 cut(s) 122
RsaI GTAC 1 cut(s) 977
RsaNI GTAC 1 cut(s) 976
SaqAI TTAA 4 cut(s) 6, 804, 951, 1096
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 3 cut(s) 184, 370, 981
SchI GAGTC 2 cut(s) 635, 953
ScrFI CCNGG 1 cut(s) 885
SduI GDGCHC 1 cut(s) 872
SfaNI GCATC 2 cut(s) 552, 682
SfcI CTRYAG 1 cut(s) 795
SmlI CTYRAG 1 cut(s) 643
SmoI CTYRAG 1 cut(s) 643
SsiI CCGC 1 cut(s) 497
SspI AATATT 1 cut(s) 1047
StyD4I CCNGG 1 cut(s) 883
TaqI TCGA 1 cut(s) 690
TatI WGTACW 1 cut(s) 975
TfiI GAWTC 3 cut(s) 14, 359, 726
Tru1I TTAA 4 cut(s) 6, 804, 951, 1096
Tru9I TTAA 4 cut(s) 6, 804, 951, 1096
TscAI CASTG 1 cut(s) 361
TseFI GTSAC 2 cut(s) 499, 1060
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 2 cut(s) 499, 1060
TspDTI ATGAA 5 cut(s) 428, 507, 632, 753, 864
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 1 cut(s) 361
VneI GTGCAC 1 cut(s) 868
XapI RAATTY 3 cut(s) 89, 115, 1072
XceI RCATGY 4 cut(s) 427, 671, 841, 1030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.