MD15G1079900.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006234
Physical Location & Seq
Forward (+)
5440126 .. 5441348
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1079900.v1.1.491

Sequence Viewer

Length: 696 bp
ATGGAGCAGAAAGAGGTGGTGATAAAAGAAAGCAGTGGCAAGGTTGGTGACCGTGATCGTGATGGCTGGGGCGATGAATCGGATCATGGGGTGCACACTTGGACTGAGAGGGAGAGAAGGAAGAAGATGAGGAGTATGTTCTCCAACCTTCATGCTTTGCTTCCTCAGCTTCCTGCTAAGGCAGATAAGTCCACCATTGTTGATGAGGCAATAAGATACATCAAATCCCTTGAGCACACTCTCCAAACAACACAAACACAAGGGCTTGACAAATTTTCTGCATTGACAGCATCAAACACAGAGTTAACTTGTGATACAAGGGAAGCATTTTTGGCTGATCATTTTCATCAGGGTCCACCAACATCAAACAACTTGGCATTGCCGGCTTCTCTTTTTCCGCCTTCCTTCCAGACGTGGTTTTCCCCAAACTTTGTCATGAACATGAGCGGCAACGATGCTCAGATCAGCGTTTGCTCACCACCAAAGCCAGGGCTCTTGACTACTATGTTTTACATACTAGAGAAGCATAAGCTGGATGTAGTATCTGCTCACGTATCCTCGGATCGATATCGTTGCATGTACATGATCCATGCTCATGCGGGTGCAGCTTGTGATAATTTTCCAGAGGCATTGTCAGTGGAGGACATATTCAAACTAGCAGCAGGTGAAATGAACTTGTGGCTCTTGTCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

232

Amino Acids

25.89

Weight (kDa)

5.91

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 32 - 78 1.8e-11 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 447
AciI CCGC 3 cut(s) 398, 447, 599
AclWI GGATC 3 cut(s) 90, 570, 580
AcsI RAATTY 1 cut(s) 272
AfaI GTAC 1 cut(s) 581
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 1 cut(s) 652
AjiI CACGTC 1 cut(s) 414
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 3 cut(s) 169, 532, 608
AluI AGCT 3 cut(s) 169, 532, 608
Alw21I GWGCWC 2 cut(s) 96, 237
Alw44I GTGCAC 1 cut(s) 92
AlwI GGATC 3 cut(s) 90, 570, 580
ApaLI GTGCAC 1 cut(s) 92
ApeKI GCWGC 2 cut(s) 605, 659
ApoI RAATTY 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 4 cut(s) 31, 59, 468, 677
AvaII GGWCC 1 cut(s) 353
BaeGI GKGCMC 1 cut(s) 96
BanII GRGCYC 1 cut(s) 495
Bbv12I GWGCWC 2 cut(s) 96, 237
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 2 cut(s) 617, 671
BccI CCATC 1 cut(s) 56
BcgI CGANNNNNNTGC 2 cut(s) 555, 589
BciT130I CCWGG 1 cut(s) 489
BciVI GTATCC 1 cut(s) 565
BclI TGATCA 1 cut(s) 337
BfaI CTAG 2 cut(s) 518, 656
BfuAI ACCTGC 1 cut(s) 653
BfuI GTATCC 1 cut(s) 565
BisI GCNGC 3 cut(s) 448, 606, 660
BlsI GCNGC 3 cut(s) 449, 607, 661
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 1 cut(s) 353
BmgBI CACGTC 1 cut(s) 414
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 1 cut(s) 489
BmsI GCATC 2 cut(s) 299, 445
Bpu10I CCTNAGC 2 cut(s) 165, 177
BpuEI CTTGAG 1 cut(s) 251
Bsa29I ATCGAT 1 cut(s) 565
BsaAI YACGTR 1 cut(s) 553
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 2 cut(s) 488, 558
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse118I RCCGGY 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 377
Bse8I GATNNNNATC 1 cut(s) 567
BseBI CCWGG 1 cut(s) 489
BseCI ATCGAT 1 cut(s) 565
BseDI CCNNGG 2 cut(s) 488, 558
BseGI GGATG 1 cut(s) 541
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMI GCAATG 1 cut(s) 377
BseMII CTCAG 3 cut(s) 96, 179, 473
BseRI GAGGAG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 96
BseXI GCAGC 2 cut(s) 617, 671
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 624
BshVI ATCGAT 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 96, 237
BsiSI CCGG 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 488
Bsp1286I GDGCHC 3 cut(s) 96, 237, 495
Bsp1407I TGTACA 1 cut(s) 579
Bsp143I GATC 6 cut(s) 55, 82, 337, 462, 562, 585
BspACI CCGC 3 cut(s) 398, 447, 599
BspCNI CTCAG 3 cut(s) 97, 178, 472
BspDI ATCGAT 1 cut(s) 565
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 354
BspMI ACCTGC 1 cut(s) 653
BspPI GGATC 3 cut(s) 90, 570, 580
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 377
BsrFI RCCGGY 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 579
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 2 cut(s) 488, 558
BssMI GATC 6 cut(s) 55, 82, 337, 462, 562, 585
Bst2UI CCWGG 1 cut(s) 489
Bst4CI ACNGT 1 cut(s) 53
BstAUI TGTACA 1 cut(s) 579
BstBAI YACGTR 1 cut(s) 553
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 4 cut(s) 105, 165, 177, 459
BstEII GGTNACC 1 cut(s) 47
BstF5I GGATG 1 cut(s) 541
BstKTI GATC 6 cut(s) 58, 85, 340, 465, 565, 588
BstMBI GATC 6 cut(s) 55, 82, 337, 462, 562, 585
BstMWI GCNNNNNNNGC 5 cut(s) 166, 287, 332, 383, 605
BstNI CCWGG 1 cut(s) 489
BstNSI RCATGY 1 cut(s) 580
BstPI GGTNACC 1 cut(s) 47
BstSCI CCNGG 1 cut(s) 487
BstSLI GKGCMC 1 cut(s) 96
BstV1I GCAGC 2 cut(s) 617, 671
Bsu15I ATCGAT 1 cut(s) 565
BsuI GTATCC 1 cut(s) 565
BsuTUI ATCGAT 1 cut(s) 565
BtgZI GCGATG 1 cut(s) 87
BtrI CACGTC 1 cut(s) 414
BtsCI GGATG 1 cut(s) 541
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 40, 642
BveI ACCTGC 1 cut(s) 653
Cac8I GCNNGC 1 cut(s) 384
CciI TCATGA 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 1 cut(s) 353
ClaI ATCGAT 1 cut(s) 565
Csp6I GTAC 1 cut(s) 580
CspCI CAANNNNNGTGG 2 cut(s) 181, 216
CviAII CATG 9 cut(s) 86, 152, 436, 442, 577, 583, 590, 596, 690
CviQI GTAC 1 cut(s) 580
DdeI CTNAG 4 cut(s) 105, 165, 177, 459
DpnI GATC 6 cut(s) 57, 84, 339, 464, 564, 587
DpnII GATC 6 cut(s) 55, 82, 337, 462, 562, 585
EciI GGCGGA 1 cut(s) 387
Eco24I GRGCYC 1 cut(s) 495
Eco32I GATATC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 353
Eco91I GGTNACC 1 cut(s) 47
EcoO65I GGTNACC 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 487
EcoRV GATATC 1 cut(s) 569
EcoT38I GRGCYC 1 cut(s) 495
FaeI CATG 9 cut(s) 89, 155, 439, 445, 580, 586, 593, 599, 693
FatI CATG 9 cut(s) 85, 151, 435, 441, 576, 582, 589, 595, 689
FauI CCCGC 1 cut(s) 592
FbaI TGATCA 1 cut(s) 337
Fnu4HI GCNGC 3 cut(s) 448, 606, 660
FokI GGATG 1 cut(s) 548
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 3 cut(s) 448, 606, 660
FspBI CTAG 2 cut(s) 518, 656
GluI GCNGC 3 cut(s) 448, 606, 660
GsaI CCCAGC 1 cut(s) 70
HapII CCGG 1 cut(s) 383
Hin1II CATG 9 cut(s) 89, 155, 439, 445, 580, 586, 593, 599, 693
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 1 cut(s) 77
HpaI GTTAAC 1 cut(s) 306
HpaII CCGG 1 cut(s) 383
HphI GGTGA 4 cut(s) 31, 59, 468, 677
Hpy166II GTNNAC 4 cut(s) 94, 192, 306, 356
Hpy188I TCNGA 3 cut(s) 82, 462, 562
Hpy188III TCNNGA 5 cut(s) 59, 409, 436, 496, 623
Hpy8I GTNNAC 4 cut(s) 94, 192, 306, 356
HpyAV CCTTC 4 cut(s) 111, 158, 411, 415
HpyCH4III ACNGT 1 cut(s) 53
HpyCH4IV ACGT 2 cut(s) 413, 552
HpyCH4V TGCA 4 cut(s) 94, 281, 576, 605
HpyF10VI GCNNNNNNNGC 5 cut(s) 166, 287, 332, 383, 605
HpyF3I CTNAG 4 cut(s) 105, 165, 177, 459
HpySE526I ACGT 2 cut(s) 413, 552
Hsp92II CATG 9 cut(s) 89, 155, 439, 445, 580, 586, 593, 599, 693
KroI GCCGGC 1 cut(s) 382
KroNI GCCGGC 1 cut(s) 384
Ksp22I TGATCA 1 cut(s) 337
KspAI GTTAAC 1 cut(s) 306
Kzo9I GATC 6 cut(s) 55, 82, 337, 462, 562, 585
LmnI GCTCC 1 cut(s) 4
Lsp1109I GCAGC 2 cut(s) 617, 671
LweI GCATC 2 cut(s) 299, 445
MaeI CTAG 2 cut(s) 518, 656
MaeII ACGT 2 cut(s) 413, 552
MaeIII GTNAC 1 cut(s) 47
MalI GATC 6 cut(s) 57, 84, 339, 464, 564, 587
MbiI CCGCTC 1 cut(s) 447
MboI GATC 6 cut(s) 55, 82, 337, 462, 562, 585
MboII GAAGA 2 cut(s) 133, 136
MhlI GDGCHC 3 cut(s) 96, 237, 495
MluCI AATT 2 cut(s) 272, 616
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 8 cut(s) 7, 102, 123, 174, 199, 568, 619, 634
MroNI GCCGGC 1 cut(s) 382
MseI TTAA 1 cut(s) 305
MslI CAYNNNNRTG 4 cut(s) 440, 581, 594, 600
MspI CCGG 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 5 cut(s) 166, 287, 332, 383, 605
NaeI GCCGGC 1 cut(s) 384
NdeII GATC 6 cut(s) 55, 82, 337, 462, 562, 585
NgoMIV GCCGGC 1 cut(s) 382
NlaIII CATG 9 cut(s) 89, 155, 439, 445, 580, 586, 593, 599, 693
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 47
NspI RCATGY 1 cut(s) 580
PagI TCATGA 1 cut(s) 435
PaqCI CACCTGC 1 cut(s) 653
PdiI GCCGGC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 77
PkrI GCNGC 3 cut(s) 449, 607, 661
Ppu21I YACGTR 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 487
PspEI GGTNACC 1 cut(s) 47
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 1 cut(s) 353
RsaI GTAC 1 cut(s) 581
RsaNI GTAC 1 cut(s) 580
RseI CAYNNNNRTG 4 cut(s) 440, 581, 594, 600
SaqAI TTAA 1 cut(s) 305
SatI GCNGC 3 cut(s) 448, 606, 660
Sau3AI GATC 6 cut(s) 55, 82, 337, 462, 562, 585
Sau96I GGNCC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 3 cut(s) 96, 237, 495
SetI ASST 9 cut(s) 18, 45, 150, 171, 416, 534, 555, 610, 667
SfaNI GCATC 2 cut(s) 299, 445
SinI GGWCC 1 cut(s) 353
SmiMI CAYNNNNRTG 4 cut(s) 440, 581, 594, 600
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
Sse9I AATT 2 cut(s) 272, 616
SsiI CCGC 3 cut(s) 398, 447, 599
SspMI CTAG 2 cut(s) 518, 656
StyD4I CCNGG 1 cut(s) 487
TaaI ACNGT 1 cut(s) 53
TaiI ACGT 2 cut(s) 416, 555
TaqI TCGA 1 cut(s) 565
TasI AATT 2 cut(s) 272, 616
TatI WGTACW 1 cut(s) 579
TauI GCSGC 1 cut(s) 450
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 1 cut(s) 305
Tru9I TTAA 1 cut(s) 305
TscAI CASTG 2 cut(s) 40, 642
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 2 cut(s) 605, 659
Tsp45I GTSAC 1 cut(s) 47
TspDTI ATGAA 5 cut(s) 90, 140, 335, 452, 686
TspRI CASTG 2 cut(s) 40, 642
VneI GTGCAC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 353
XapI RAATTY 1 cut(s) 272
XceI RCATGY 1 cut(s) 580
XspI CTAG 2 cut(s) 518, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.