Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Forward (+)

19499146 .. 19506309

Gene Structure 7164 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G52360.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2781 bp

ATGCCGCTCAGACTCGAGATCAAGAGAAAATTAGCGCAAAGGTCTGAGAGAGTTAAATCTGTGGATCTTCATCCTACAGAACCATGGATTCTTGCAAGTTTGTATTCTGGAACCTTGTGTATTTGGAACTACCAGACGCAGGTGATGGCAAAATCCTTTGAGGTGACTGAATTACCAGTTCGGTCAGCCAAGTTTGTAGCACGAAAGCAATGGGTTGTCGCAGGAGCTGATGATATGTATATCCGTGTATACAATTACAACACCATGGACAAGGTTAAAGTGTTTGAGGCTCATTCAGACTACATTAGGTGTGTGGCTGTTCATCCAACCCTTCCATATGTGCTGTCATCTTCTGATGATATGCTCATAAAGCTTTGGGACTGGGAAAAGGGTTGGGCTTGCACTCAGATATTCGAGGGACACTCACACTATGTGATGCAAGTCACATTTAATCCAAAAGACACCAACACTTTTGCCAGTGCATCCCTTGATCGTACCATAAAGATTTGGAATCTTGGTTCCCCAGACCCAAATTTTACATTGGATGCCCATCAGAAAGGAGTAAATTGTGTAGATTATTTCACCGGTGGTGATAAGCCCTATTTAATTACCGGCTCTGATGATCACACTGCTAAGGTCTGGGACTACCAAACAAAAAGTTGTGTACAGACCCTGGAGGGGCACACACACAATGTATCTGCAGTATGTTTCCATCCAGAGCTTCCAATTATAATCACAGGTTCTGAAGATGGCACCGTTCGCATTTGGCATGCAACCACATACAGGCTAGAGAACACATTGAATTATGGCCTTGAGAGAGTCTGGGCAATTGGTTACATTAAAAGTTCACGCCGGGTTGTGATCGGATATGATGAAGGAACCATCATGGTTAAACTTGGACGAGAAATTCCTGTCGCTAGCATGGACAATACCGGAAAAATCATATGGGCTAAGCATAATGAAATTCAAACTGCAAACATCAAAAGTATTGGTGCAGATTACGAGGTTACTGATGGAGAAAGGTTGCCCTTGAGTGTTAAAGAGTTGGGGACCTGTGATCTTTATCCACAAAGCTTGAAGCACAACCCTAATGGGAGGTTTGTCGTAGTCTGTGGTGACGGAGAATATATAATCTACACGGCTTTGGCTTGGAGAAATAGGTCATTTGGTTCTGGACTGGAATTTGTTTGGTCATCCGAGGGGGAGTGTGCGGTTAGAGAAAGCTCATCAAAGATTAAAATATTTAGCAAAAATTTCCAGGAAAAGAGGAGTATCCGCCCTACTTTTTCAGCTGAGAAGATCTTTGGAGGAACCTTGTTAGCTATGTGTTCAAGTGATTTCATCTGCTTTTATGATTGGGCTGAATGTAGGCTGATTCAACGTATTGACGTCACTGTAAAGAATCTATATTGGGCTGACAGTGGTGATTTAGTAGCCATTGCTAGTGACACATCATTCTACATCTTGAAATTCAACCGCGACTTAGTTACCTCACATTTTGATAGCGGAAGACCTACTGAGGAAGAAGGTGTTGAGGATGCTTTTGAGGTTCTCCATGAAAATGATGAACGTGTTAGGACAGGTATATGGGTTGGTGACTGTTTCATTTACAACAACTCTTCCTGGAAGCTTAACTACTGTGTTGGAGGCGAGGTAACCACAATGTATCATTTGGACCGCCCAATGTATTTGTTGGGATATCTTGCAAGTCAAAGTCGGGTTTTCTTGGTAGACAAAGAATTCAATGTTATAGGGTACACCTTGTTGCTAAGCCTGATTGAGTACAAGACTCTTGTGATGCGGGGTGATTTAGACAAAGCCAGTGAAATCTTACCTACAATTCCTAAAGATCAGCATAACAGTGTTGCTCATTTCTTGGAGTCACGGGGAATGATTGAAGATGCTCTGGAAATTGCAACAGATCCTGACTACAGATTTGAGTTGGCCATACAACTGGGTAGACTTGAAATTGCACAGGAAATCGCTGTGGAAGTACAGAGCGAATCTAAGTGGAAACAATTAGGAGAGTTAGCTATGTCCTCCGGAAAGCTGCAAATGGCTGAGGAATGCATGAAGTATGCGATGGATTTGAGTGGTTTGTTACTTCTTTATTCTTCTCTGGGAGATGCTGAAGGTGTAACAAAACTTGCAACACTTGCTAAAGAACAAGGGAAGAACAATGTCGCCTTTCTTTGCCTATTCATGCTGGGTAAACTGGAAGATTGTTTGCAGTTATTGGTGGAGAGTAACCGGATACCAGAAGCTGCTCTGATGGCACGATCATATCTTCCGAGCAAAGTTTCTGAGATAGTAGCTCTTTGGAGGAAAGATCTCAGCAAGGTTAATTCAAAAGCAGCAGAATCTTTGGCTGATCCTGAGGAGTACTCAAATCTTTTTGAAGATTGGCAAGTTGCTCTTTCTGTGGAAGCTAAAGCTGTAGAGACAAGGGGAGTTTACACAGGTGCAAAAGATTATCCTAGCCATGCTGATAAATCTTCCATGACCCTCGTGGAAGCCTTCCGAAACTTGCAAGTTGAGGAAGAGGAATCACTTGAAAATGGAGATATGGATCACGAGGAGGTAGTAGCAGAAGAAAATGGAAATGAACAAAGGAATGAGGATGATGTAGCGGAGCATGTAGAGGAGCATCATGAAGAGAAAGAAGCAGAAGAAGAAGAGGGAATTGTTGATGGAGACTCTACGGATGGAGCTGTACTTGTTAATGGAAGTGAGGCTGACGAAGAGTGGGGTACGAATAATGAAGGAAACCCATCAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

926

Amino Acids

104.47

Weight (kDa)

4.84

Isoelectric Point (pI)

35.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	3 - 150	5.8e-12	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	8 - 111	4.6e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	9 - 155	2.8e-16	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	12 - 154	1.3e-20	WDR5 beta-propeller domain
WDR55	PF24796	16 - 199	1.4e-14	WDR55
WD40_Prp19	PF24814	16 - 185	1.6e-19	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	16 - 174	1.1e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	20 - 172	2.6e-15	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	31 - 170	2.7e-06	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	32 - 210	1.1e-14	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	36 - 193	4.1e-16	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	58 - 131	1.7e-11	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	60 - 192	7.6e-21	THOC3 beta-propeller domain
Beta-prop_WDR5	PF25175	60 - 193	1.1e-26	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	61 - 169	6.1e-08	MABP1/WDR62 second WD40 domain
Beta-prop_EIPR1	PF23609	62 - 170	6.3e-07	EIPR1 beta-propeller
Beta-prop_TEP1_2nd	PF25047	69 - 260	1.5e-14	TEP-1 second beta-propeller
WD40	PF00400	91 - 127	5.5e-08	WD domain, G-beta repeat
WD40	PF00400	133 - 171	5.8e-07	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	134 - 296	2.7e-28	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	137 - 246	2.2e-06	RIG second beta-propeller
WDR55	PF24796	138 - 296	3.8e-16	WDR55
EIF3I	PF24805	138 - 229	2.5e-06	EIF3I
Beta-prop_WDR3_2nd	PF25172	140 - 260	1.6e-15	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	154 - 294	2.2e-30	WDR5 beta-propeller domain
Beta-prop_EML	PF23409	164 - 261	7e-08	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	177 - 215	5e-07	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_1st	PF25171	179 - 256	6.9e-06	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	183 - 273	3.7e-16	Prp19 WD40 domain
Beta-prop_SCAP	PF24017	183 - 260	2.2e-06	SCAP Beta-propeller
Beta-prop_THOC3	PF25174	184 - 296	1.3e-16	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	197 - 264	1.5e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR75_1st	PF23869	198 - 267	1.8e-06	WD repeat-containing protein 75 first beta-propeller
WD40_CDC20-Fz	PF24807	198 - 288	6.2e-09	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	198 - 318	2.3e-12	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	199 - 296	4.1e-09	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	200 - 292	6.5e-10	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	201 - 260	1.8e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 262	5.3e-07	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	220 - 256	2.2e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 298	6.5e-07	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	2.6e-89	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	2.4e-82	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	731
AarI	CACCTGC	1 cut(s)	130
AatII	GACGTC	1 cut(s)	1392
Acc36I	ACCTGC	1 cut(s)	130
AccB1I	GGYRCC	1 cut(s)	752
AccBSI	CCGCTC	1 cut(s)	7
AccI	GTMKAC	3 cut(s)	249, 1731, 1960
AccII	CGCG	1 cut(s)	1479
AccIII	TCCGGA	1 cut(s)	2042
AciI	CCGC	8 cut(s)	5, 1211, 1276, 1477, 1506, 1678, 1801, 2630
AclWI	GGATC	4 cut(s)	72, 1916, 2366, 2577
AcoI	YGGCCR	1 cut(s)	1944
AcsI	RAATTY	7 cut(s)	532, 906, 963, 1181, 1252, 1469, 1739
AcuI	CTGAAG	2 cut(s)	765, 2151
AcyI	GRCGYC	1 cut(s)	1389
AdeI	CACNNNGTG	1 cut(s)	433
AfaI	GTAC	8 cut(s)	496, 666, 1757, 1784, 1995, 2384, 2715, 2752
AfiI	CCNNNNNNNGG	3 cut(s)	139, 678, 783
AflIII	ACRYGT	1 cut(s)	1570
AgeI	ACCGGT	1 cut(s)	584
AjnI	CCWGG	3 cut(s)	672, 1257, 1622
AloI	GAACNNNNNNTCC	2 cut(s)	502, 534
Alw26I	GTCTC	2 cut(s)	2435, 2688
AlwI	GGATC	4 cut(s)	72, 1916, 2366, 2577
AlwNI	CAGNNNCTG	5 cut(s)	227, 673, 743, 1925, 2264
Ama87I	CYCGRG	1 cut(s)	14
Aor13HI	TCCGGA	1 cut(s)	2042
AoxI	GGCC	2 cut(s)	808, 1944
ApeKI	GCWGC	3 cut(s)	2050, 2264, 2354
ApoI	RAATTY	7 cut(s)	532, 906, 963, 1181, 1252, 1469, 1739
ArsI	GACNNNNNNTTYG	2 cut(s)	1919, 1951
AsiGI	ACCGGT	1 cut(s)	584
AspLEI	GCGC	1 cut(s)	37
AspS9I	GGNCC	2 cut(s)	1050, 1675
AsuC2I	CCSGG	1 cut(s)	854
AsuHPI	GGTGA	8 cut(s)	154, 175, 574, 602, 1127, 1436, 1607, 1817
AsuNHI	GCTAGC	1 cut(s)	917
AvaI	CYCGRG	1 cut(s)	14
AvaII	GGWCC	2 cut(s)	1050, 1675
AxyI	CCTNAGG	1 cut(s)	2376
BaeGI	GKGCMC	1 cut(s)	684
BalI	TGGCCA	1 cut(s)	1946
BanI	GGYRCC	1 cut(s)	752
BarI	GAAGNNNNNNTAC	2 cut(s)	1619, 1651
BauI	CACGAG	2 cut(s)	2507, 2573
BbsI	GAAGAC	1 cut(s)	1516
BbvCI	CCTCAGC	1 cut(s)	2061
BbvI	GCAGC	3 cut(s)	2037, 2251, 2366
BceAI	ACGGC	1 cut(s)	1155
BciT130I	CCWGG	3 cut(s)	674, 1259, 1624
BciVI	GTATCC	2 cut(s)	1283, 2247
BclI	TGATCA	1 cut(s)	622
BcnI	CCSGG	1 cut(s)	854
BcoDI	GTCTC	2 cut(s)	2435, 2688
BfaI	CTAG	4 cut(s)	788, 918, 1443, 2478
BfmI	CTRYAG	4 cut(s)	75, 699, 1930, 2436
BfuAI	ACCTGC	1 cut(s)	130
BfuI	GTATCC	2 cut(s)	1283, 2247
BglII	AGATCT	2 cut(s)	1299, 2329
BisI	GCNGC	4 cut(s)	5, 2051, 2265, 2355
BlpI	GCTNAGC	2 cut(s)	951, 1769
BlsI	GCNGC	4 cut(s)	6, 2052, 2266, 2356
BmcAI	AGTACT	1 cut(s)	2384
Bme1390I	CCNGG	4 cut(s)	674, 854, 1259, 1624
Bme18I	GGWCC	2 cut(s)	1050, 1675
BmeT110I	CYCGRG	1 cut(s)	14
BmgT120I	GGNCC	2 cut(s)	1050, 1675
BmiI	GGNNCC	6 cut(s)	112, 520, 754, 880, 1051, 1312
BmrFI	CCNGG	4 cut(s)	674, 854, 1259, 1624
BmrI	ACTGGG	2 cut(s)	391, 1964
BmsI	GCATC	8 cut(s)	426, 491, 535, 1528, 1788, 1891, 2116, 2656
BmtI	GCTAGC	1 cut(s)	921
BmuI	ACTGGG	2 cut(s)	391, 1964
BpiI	GAAGAC	1 cut(s)	1516
BplI	GAGNNNNNCTC	4 cut(s)	407, 439, 2369, 2401
BpmI	CTGGAG	1 cut(s)	695
Bpu10I	CCTNAGC	2 cut(s)	633, 2061
Bpu1102I	GCTNAGC	2 cut(s)	951, 1769
BpuEI	CTTGAG	2 cut(s)	833, 1051
BpuMI	CCSGG	1 cut(s)	854
BsaBI	GATNNNNATC	4 cut(s)	69, 549, 1062, 2568
BsaHI	GRCGYC	1 cut(s)	1389
BsaJI	CCNNGG	4 cut(s)	83, 264, 672, 1197
BsaWI	WCCGGW	4 cut(s)	584, 932, 2042, 2250
BsaXI	ACNNNNNCTCC	2 cut(s)	1638, 1668
Bsc4I	CCNNNNNNNGG	3 cut(s)	139, 678, 783
Bse118I	RCCGGY	2 cut(s)	584, 611
Bse1I	ACTGG	7 cut(s)	176, 386, 477, 1182, 1821, 1959, 2220
Bse21I	CCTNAGG	1 cut(s)	2376
Bse3DI	GCAATG	2 cut(s)	215, 1437
Bse8I	GATNNNNATC	4 cut(s)	69, 549, 1062, 2568
BseAI	TCCGGA	1 cut(s)	2042
BseBI	CCWGG	3 cut(s)	674, 1259, 1624
BseDI	CCNNGG	4 cut(s)	83, 264, 672, 1197
BseGI	GGATG	9 cut(s)	70, 322, 482, 550, 712, 1193, 1543, 2626, 2710
BseJI	GATNNNNATC	4 cut(s)	69, 549, 1062, 2568
BseLI	CCNNNNNNNGG	3 cut(s)	139, 678, 783
BseMI	GCAATG	2 cut(s)	215, 1437
BseMII	CTCAG	9 cut(s)	22, 36, 419, 1284, 1509, 2052, 2295, 2347, 2367
BseNI	ACTGG	7 cut(s)	176, 386, 477, 1182, 1821, 1959, 2220
BseRI	GAGGAG	4 cut(s)	1282, 2393, 2591, 2657
BseSI	GKGCMC	1 cut(s)	684
BseXI	GCAGC	3 cut(s)	2037, 2251, 2366
BseYI	CCCAGC	1 cut(s)	2206
BsgI	GTGCAG	1 cut(s)	1014
Bsh1236I	CGCG	1 cut(s)	1479
BshFI	GGCC	2 cut(s)	810, 1946
BshNI	GGYRCC	1 cut(s)	752
BshTI	ACCGGT	1 cut(s)	584
BsiHKCI	CYCGRG	1 cut(s)	14
BsiSI	CCGG	6 cut(s)	585, 612, 853, 933, 2043, 2251
BslFI	GGGAC	4 cut(s)	392, 432, 656, 1063
BslI	CCNNNNNNNGG	3 cut(s)	139, 678, 783
BsmAI	GTCTC	2 cut(s)	2435, 2688
BsmFI	GGGAC	4 cut(s)	392, 432, 656, 1063
BsmI	GAATGC	1 cut(s)	2072
BsnI	GGCC	2 cut(s)	810, 1946
BsoBI	CYCGRG	1 cut(s)	14
Bsp1286I	GDGCHC	1 cut(s)	684
Bsp13I	TCCGGA	1 cut(s)	2042
Bsp1407I	TGTACA	1 cut(s)	664
Bsp1720I	GCTNAGC	2 cut(s)	951, 1769
Bsp19I	CCATGG	2 cut(s)	83, 264
BspACI	CCGC	8 cut(s)	5, 1211, 1276, 1477, 1506, 1678, 1801, 2630
BspANI	GGCC	2 cut(s)	810, 1946
BspCNI	CTCAG	9 cut(s)	21, 37, 418, 1285, 1510, 2053, 2296, 2346, 2368
BspEI	TCCGGA	1 cut(s)	2042
BspFNI	CGCG	1 cut(s)	1479
BspHI	TCATGA	1 cut(s)	2650
BspLI	GGNNCC	6 cut(s)	112, 520, 754, 880, 1051, 1312
BspMAI	CTGCAG	1 cut(s)	703
BspMI	ACCTGC	1 cut(s)	130
BspOI	GCTAGC	1 cut(s)	921
BspPI	GGATC	4 cut(s)	72, 1916, 2366, 2577
BspT107I	GGYRCC	1 cut(s)	752
BsrBI	CCGCTC	1 cut(s)	7
BsrDI	GCAATG	2 cut(s)	215, 1437
BsrFI	RCCGGY	2 cut(s)	584, 611
BsrGI	TGTACA	1 cut(s)	664
BsrI	ACTGG	7 cut(s)	176, 386, 477, 1182, 1821, 1959, 2220
BssAI	RCCGGY	2 cut(s)	584, 611
BssECI	CCNNGG	4 cut(s)	83, 264, 672, 1197
BssNAI	GTATAC	1 cut(s)	250
BssNI	GRCGYC	1 cut(s)	1389
BssSI	CACGAG	2 cut(s)	2507, 2573
BssT1I	CCWWGG	2 cut(s)	83, 264
Bst1107I	GTATAC	1 cut(s)	250
Bst2BI	CACGAG	2 cut(s)	2507, 2573
Bst2UI	CCWGG	3 cut(s)	674, 1259, 1624
Bst4CI	ACNGT	6 cut(s)	757, 1396, 1421, 1601, 1640, 1862
Bst6I	CTCTTC	5 cut(s)	1624, 2535, 2649, 2670, 2736
BstACI	GRCGYC	1 cut(s)	1389
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstAUI	TGTACA	1 cut(s)	664
BstC8I	GCNNGC	3 cut(s)	400, 771, 919
BstDSI	CCRYGG	2 cut(s)	83, 264
BstEII	GGTNACC	1 cut(s)	1654
BstF5I	GGATG	9 cut(s)	70, 322, 482, 550, 712, 1193, 1543, 2626, 2710
BstFNI	CGCG	1 cut(s)	1479
BstHHI	GCGC	1 cut(s)	37
BstMAI	GTCTC	2 cut(s)	2435, 2688
BstMWI	GCNNNNNNNGC	4 cut(s)	370, 759, 2156, 2273
BstNI	CCWGG	3 cut(s)	674, 1259, 1624
BstNSI	RCATGY	2 cut(s)	773, 2639
BstPI	GGTNACC	1 cut(s)	1654
BstSCI	CCNGG	4 cut(s)	672, 852, 1257, 1622
BstSFI	CTRYAG	4 cut(s)	75, 699, 1930, 2436
BstSLI	GKGCMC	1 cut(s)	684
BstUI	CGCG	1 cut(s)	1479
BstV1I	GCAGC	3 cut(s)	2037, 2251, 2366
BstV2I	GAAGAC	1 cut(s)	1516
BstX2I	RGATCY	4 cut(s)	64, 1299, 1921, 2329
BstXI	CCANNNNNNTGG	1 cut(s)	1954
BstYI	RGATCY	4 cut(s)	64, 1299, 1921, 2329
BstZ17I	GTATAC	1 cut(s)	250
Bsu36I	CCTNAGG	1 cut(s)	2376
BsuI	GTATCC	2 cut(s)	1283, 2247
BsuRI	GGCC	2 cut(s)	810, 1946
BtgI	CCRYGG	2 cut(s)	83, 264
BtgZI	GCGATG	1 cut(s)	2096
BtsCI	GGATG	9 cut(s)	70, 322, 482, 550, 712, 1193, 1543, 2626, 2710
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	6 cut(s)	484, 627, 1392, 1426, 1828, 1867
BveI	ACCTGC	1 cut(s)	130
Cac8I	GCNNGC	3 cut(s)	400, 771, 919
CaiI	CAGNNNCTG	5 cut(s)	227, 673, 743, 1925, 2264
CciI	TCATGA	1 cut(s)	2650
CfoI	GCGC	1 cut(s)	37
Cfr10I	RCCGGY	2 cut(s)	584, 611
Cfr13I	GGNCC	2 cut(s)	1050, 1675
CseI	GACGC	1 cut(s)	145
Csp6I	GTAC	8 cut(s)	495, 665, 1756, 1783, 1994, 2383, 2714, 2751
CspAI	ACCGGT	1 cut(s)	584
CspCI	CAANNNNNGTGG	2 cut(s)	1056, 1091
CviQI	GTAC	8 cut(s)	495, 665, 1756, 1783, 1994, 2383, 2714, 2751
DraIII	CACNNNGTG	1 cut(s)	433
EaeI	YGGCCR	1 cut(s)	1944
Eam1104I	CTCTTC	5 cut(s)	1624, 2535, 2649, 2670, 2736
EarI	CTCTTC	5 cut(s)	1624, 2535, 2649, 2670, 2736
EciI	GGCGGA	1 cut(s)	1265
Eco130I	CCWWGG	2 cut(s)	83, 264
Eco32I	GATATC	1 cut(s)	1700
Eco47I	GGWCC	2 cut(s)	1050, 1675
Eco57I	CTGAAG	2 cut(s)	765, 2151
Eco81I	CCTNAGG	1 cut(s)	2376
Eco88I	CYCGRG	1 cut(s)	14
Eco91I	GGTNACC	1 cut(s)	1654
EcoO109I	RGGNCCY	1 cut(s)	1050
EcoO65I	GGTNACC	1 cut(s)	1654
EcoRI	GAATTC	1 cut(s)	1739
EcoRII	CCWGG	3 cut(s)	672, 1257, 1622
EcoRV	GATATC	1 cut(s)	1700
EcoT14I	CCWWGG	2 cut(s)	83, 264
EcoT22I	ATGCAT	1 cut(s)	2072
ErhI	CCWWGG	2 cut(s)	83, 264
FalI	AAGNNNNNCTT	2 cut(s)	2400, 2432
FaqI	GGGAC	4 cut(s)	392, 432, 656, 1063
FauI	CCCGC	1 cut(s)	1794
FauNDI	CATATG	2 cut(s)	337, 944
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	3 cut(s)	249, 1731, 1960
Fnu4HI	GCNGC	4 cut(s)	5, 2051, 2265, 2355
FokI	GGATG	9 cut(s)	57, 309, 469, 557, 699, 1180, 1550, 2633, 2717
Fsp4HI	GCNGC	4 cut(s)	5, 2051, 2265, 2355
FspBI	CTAG	4 cut(s)	788, 918, 1443, 2478
GlaI	GCGC	1 cut(s)	36
GluI	GCNGC	4 cut(s)	5, 2051, 2265, 2355
GsaI	CCCAGC	1 cut(s)	2210
GsuI	CTGGAG	1 cut(s)	695
HaeIII	GGCC	2 cut(s)	810, 1946
HapII	CCGG	6 cut(s)	585, 612, 853, 933, 2043, 2251
HgaI	GACGC	1 cut(s)	145
HhaI	GCGC	1 cut(s)	37
Hin1I	GRCGYC	1 cut(s)	1389
Hin6I	GCGC	1 cut(s)	35
HinP1I	GCGC	1 cut(s)	35
HindIII	AAGCTT	3 cut(s)	371, 1072, 1628
HpaII	CCGG	6 cut(s)	585, 612, 853, 933, 2043, 2251
HphI	GGTGA	8 cut(s)	154, 175, 574, 602, 1127, 1436, 1607, 1817
Hpy166II	GTNNAC	8 cut(s)	250, 665, 848, 1732, 1758, 1961, 2213, 2455
Hpy8I	GTNNAC	8 cut(s)	250, 665, 848, 1732, 1758, 1961, 2213, 2455
HpyAV	CCTTC	6 cut(s)	341, 869, 1520, 2126, 2527, 2756
HpyCH4III	ACNGT	6 cut(s)	757, 1396, 1421, 1601, 1640, 1862
HpyCH4IV	ACGT	3 cut(s)	1381, 1389, 1570
HpyF10VI	GCNNNNNNNGC	4 cut(s)	370, 759, 2156, 2273
HpySE526I	ACGT	3 cut(s)	1381, 1389, 1570
Hsp92I	GRCGYC	1 cut(s)	1389
HspAI	GCGC	1 cut(s)	35
Kpn2I	TCCGGA	1 cut(s)	2042
Ksp22I	TGATCA	1 cut(s)	622
LmnI	GCTCC	4 cut(s)	224, 2632, 2644, 2708
Lsp1109I	GCAGC	3 cut(s)	2037, 2251, 2366
LweI	GCATC	8 cut(s)	426, 491, 535, 1528, 1788, 1891, 2116, 2656
MaeI	CTAG	4 cut(s)	788, 918, 1443, 2478
MaeII	ACGT	3 cut(s)	1381, 1389, 1570
MbiI	CCGCTC	1 cut(s)	7
MfeI	CAATTG	1 cut(s)	828
MflI	RGATCY	4 cut(s)	64, 1299, 1921, 2329
MhlI	GDGCHC	1 cut(s)	684
MlsI	TGGCCA	1 cut(s)	1946
MluNI	TGGCCA	1 cut(s)	1946
MlyI	GAGTC	5 cut(s)	6, 828, 1783, 1889, 2690
MmeI	TCCRAC	2 cut(s)	350, 1624
Mox20I	TGGCCA	1 cut(s)	1946
Mph1103I	ATGCAT	1 cut(s)	2072
MroI	TCCGGA	1 cut(s)	2042
MscI	TGGCCA	1 cut(s)	1946
MslI	CAYNNNNRTG	2 cut(s)	1560, 1860
Msp20I	TGGCCA	1 cut(s)	1946
MspA1I	CMGCKG	1 cut(s)	1292
MspI	CCGG	6 cut(s)	585, 612, 853, 933, 2043, 2251
MspR9I	CCNGG	4 cut(s)	674, 854, 1259, 1624
MunI	CAATTG	1 cut(s)	828
Mva1269I	GAATGC	1 cut(s)	2072
MvaI	CCWGG	3 cut(s)	674, 1259, 1624
MvnI	CGCG	1 cut(s)	1479
MwoI	GCNNNNNNNGC	4 cut(s)	370, 759, 2156, 2273
NciI	CCSGG	1 cut(s)	854
NcoI	CCATGG	2 cut(s)	83, 264
NdeI	CATATG	2 cut(s)	337, 944
NheI	GCTAGC	1 cut(s)	917
NlaIV	GGNNCC	6 cut(s)	112, 520, 754, 880, 1051, 1312
NmuCI	GTSAC	7 cut(s)	163, 442, 1115, 1390, 1445, 1595, 1881
NsiI	ATGCAT	1 cut(s)	2072
NspI	RCATGY	2 cut(s)	773, 2639
PaeI	GCATGC	1 cut(s)	773
PaeR7I	CTCGAG	1 cut(s)	14
PagI	TCATGA	1 cut(s)	2650
PaqCI	CACCTGC	1 cut(s)	130
PctI	GAATGC	1 cut(s)	2072
PfeI	GAWTC	7 cut(s)	88, 511, 1375, 1402, 2003, 2360, 2546
PfoI	TCCNGGA	2 cut(s)	1257, 1622
PinAI	ACCGGT	1 cut(s)	584
PkrI	GCNGC	4 cut(s)	6, 2052, 2266, 2356
PleI	GAGTC	5 cut(s)	6, 827, 1783, 1888, 2690
PpsI	GAGTC	5 cut(s)	6, 827, 1783, 1888, 2690
PpuMI	RGGWCCY	1 cut(s)	1050
PsiI	TTATAA	1 cut(s)	731
Psp5II	RGGWCCY	1 cut(s)	1050
Psp6I	CCWGG	3 cut(s)	672, 1257, 1622
PspEI	GGTNACC	1 cut(s)	1654
PspFI	CCCAGC	1 cut(s)	2206
PspGI	CCWGG	3 cut(s)	672, 1257, 1622
PspN4I	GGNNCC	6 cut(s)	112, 520, 754, 880, 1051, 1312
PspPI	GGNCC	2 cut(s)	1050, 1675
PspPPI	RGGWCCY	1 cut(s)	1050
PstI	CTGCAG	1 cut(s)	703
PstNI	CAGNNNCTG	5 cut(s)	227, 673, 743, 1925, 2264
PsuI	RGATCY	4 cut(s)	64, 1299, 1921, 2329
PvuII	CAGCTG	1 cut(s)	1292
RsaI	GTAC	8 cut(s)	496, 666, 1757, 1784, 1995, 2384, 2715, 2752
RsaNI	GTAC	8 cut(s)	495, 665, 1756, 1783, 1994, 2383, 2714, 2751
RseI	CAYNNNNRTG	2 cut(s)	1560, 1860
SatI	GCNGC	4 cut(s)	5, 2051, 2265, 2355
Sau96I	GGNCC	2 cut(s)	1050, 1675
ScaI	AGTACT	1 cut(s)	2384
SchI	GAGTC	5 cut(s)	6, 828, 1783, 1889, 2690
ScrFI	CCNGG	4 cut(s)	674, 854, 1259, 1624
SduI	GDGCHC	1 cut(s)	684
SfaNI	GCATC	8 cut(s)	426, 491, 535, 1528, 1788, 1891, 2116, 2656
SfcI	CTRYAG	4 cut(s)	75, 699, 1930, 2436
Sfr274I	CTCGAG	1 cut(s)	14
SgrAI	CRCCGGYG	1 cut(s)	584
SinI	GGWCC	2 cut(s)	1050, 1675
SlaI	CTCGAG	1 cut(s)	14
SmiMI	CAYNNNNRTG	2 cut(s)	1560, 1860
SmlI	CTYRAG	3 cut(s)	14, 812, 1030
SmoI	CTYRAG	3 cut(s)	14, 812, 1030
SphI	GCATGC	1 cut(s)	773
SsiI	CCGC	8 cut(s)	5, 1211, 1276, 1477, 1506, 1678, 1801, 2630
SspI	AATATT	1 cut(s)	1242
SspMI	CTAG	4 cut(s)	788, 918, 1443, 2478
StyD4I	CCNGG	4 cut(s)	672, 852, 1257, 1622
StyI	CCWWGG	2 cut(s)	83, 264
TaaI	ACNGT	6 cut(s)	757, 1396, 1421, 1601, 1640, 1862
TaiI	ACGT	3 cut(s)	1384, 1392, 1573
TaqI	TCGA	2 cut(s)	15, 414
TaqII	GACCGA	1 cut(s)	171
TatI	WGTACW	5 cut(s)	664, 1782, 1993, 2382, 2713
TauI	GCSGC	1 cut(s)	7
TfiI	GAWTC	7 cut(s)	88, 511, 1375, 1402, 2003, 2360, 2546
TscAI	CASTG	6 cut(s)	484, 634, 1399, 1426, 1828, 1867
TseFI	GTSAC	7 cut(s)	163, 442, 1115, 1390, 1445, 1595, 1881
TseI	GCWGC	3 cut(s)	2050, 2264, 2354
Tsp45I	GTSAC	7 cut(s)	163, 442, 1115, 1390, 1445, 1595, 1881
TspGWI	ACGGA	3 cut(s)	233, 1134, 2717
TspRI	CASTG	6 cut(s)	484, 634, 1399, 1426, 1828, 1867
VpaK11BI	GGWCC	2 cut(s)	1050, 1675
XapI	RAATTY	7 cut(s)	532, 906, 963, 1181, 1252, 1469, 1739
XceI	RCATGY	2 cut(s)	773, 2639
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2506
XhoI	CTCGAG	1 cut(s)	14
XmiI	GTMKAC	3 cut(s)	249, 1731, 1960
XspI	CTAG	4 cut(s)	788, 918, 1443, 2478
ZraI	GACGTC	1 cut(s)	1390
ZrmI	AGTACT	1 cut(s)	2384
Zsp2I	ATGCAT	1 cut(s)	2072

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.