Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Forward (+)

2744920 .. 2754520

Gene Structure 9601 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g05170.t4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGCCTCTCAGGCTTGACATTAAGCGTAAGTTTGCGCAAAGATCCGAGAGAGTGAAGTCTTTGGATCTACATCCAACTGAACCATGGATCCTTGCAAGTCTGTACTCAGGGACCGTGTATATCTTGAACTACCAGTCACAGACTATGGCTAAGTCATTTGAAGTCTCTGAATTGCCAGTTCGGTCAGCAAAATTTGTAACACGAAAGCAATGGGTAGTTGCTGCAGCCGATGACATGTGTTTACGCGTATACAATTACAATACCATGGATAAGGTTAAAGTCTTCGAGGCGCACACTGACTACATTAGATGTGTGGCTGTCCATCCCACGCTTCCATATGTTTTATCATCTTCTGATGACATGCTCATCAAACTGTGGGACTGGGAAAAAGGGTGGATGTGTACTCAGATATTTGAAGGGCATTCTCACTATGTCATGCAAGTGACATTTAATCCCAAAGACACCAACACTTTTGCGAGTGCTTCCCTCGATCACACTGTAAAGATTTGGAATCTTGGCTCGCCTGACCCAAACTTTACATTGGACGCCCACCTGAAAGGAGTGAATTGCGTTGATTACTTCACTGGTGGTGATAAGCCGTACTTAATCACAGGATCTGATGATCATACTGCAAAGGTATGGGACTATCAAACCAAAAGTTGTGTTCAGACACTTGAAGGACACACTCATAATGTATCTGCAGTATGCTTCCACCCTGAACTTCCGATTATAATTACTGGTTCTGAGGATGGAACTGTTAGGATTTGGCATTCGACCACTTACAGGCTTGAAAACACCTTGAATTATGGGCTGGAACGTGTTTGGGCTTTTGGGTATATGAAAGGATCACGTCGGATTGTTATTGGTTATGACGAAGGAACCATTTTGGTAAAAATTGGTAGAGAAGTGCCAGTTGCTAGCATGGATAGTAGTGGGAAAATAATCTGGGCCAAGCATAATGAAATCCAAACTGTGAATATCAAAAGTGTGGGAGCAGATTTTGAGATTACAGATGGAGAAAGGTTACCATTGGCCGTGAAGGAGTTGGGAACCTGTGATCTTTATCCTCAAAATTTGAAGCACAACCCCAATGGAAGGTTTGTTGTTGTTTGTGGAGATGGAGAGTACATAATATACACGGCTCTAGCATGGAGAAATAGATCCTTTGGCTCCGCATTGGAGTTTGTCTGGTCGTCAGACGGAGAATATGCTGTTAGGGAAACGACATCAAAGATCAAAATTTTCAGCAAAACATTCCAGGAAAAGAAGAATATTCGACCAACATTTTCTGTTGAACACATTTATGGGGGGACACTATTGGCAATGTGTTCAAATGACTTTGTCTGCTTCTATGACTGGGTGGATTGCAGATTGATTAGACGAATTGATGTCAATGTGAAGAATGTTTATTGGGCTGATAGCGGTGATCTGGTGGCCATTGCTAGTGATTCATCATTTTACATTCTGAAATATGATAGGGATATAGTCTCTTCTTATTTTGAGAGTGGAAGGCCAGTTGATGAACTAGGTGTTGAAGATGCTTTTGAGCTCCTCTTTGAGATAAACGAGCGTGTCCGAACTGGATTATGGGTTGGCGACTGTTTTGTTTATAATAACTCCTCCTGGCGGCTCAACTACTGTGTTGGTGGAGAGGTGACCACAATGTTTCATTTGGACCGGCCCATGTACTTGTTGGGATATCTTGCCAATCAAAGTCGTCTTTTTCTTATTGATAAAGAGTTCAATGTCATGGGATACTCCTTGCTTCTCAGCTTGATTGAGTACAAGACACTTGTTATGCGTGGAGATTTAGAGCGTGCCAAAGAGATTTTTCCTACAATTCCCCAAGAGCAACATAACAGTGTGGCCCGATTTTTGGAATCTCGTGGGATGTTGGAGGATGCACTGGAAGTGGCTACAGATCCTGACTACAAATTTGATCTTGCTATACAGCTGGGTAATCTAGAGATTGCAAAGGAAATTGCTACTAAAGCACAGAGTGAATCCAAATGGAAGCAGCTGGGAGATTTAGCTCTGTCCTCTGGGAAGTTAGAACTTGCTGAAGATTGCCTATTACATGGACTGGACTATTCTGGCTTGTTACTTCTGTATTCTTCTCTTGGAGATGCTCAAGGAATATCAAAACTTGCTTCACTTGCTAAAGAGCAAGGAAAGAATAATGTTGCATTCCTTTGTTTGTTCATGCTGGGTAAATTGGAAGAATGCATCCAGCTGTTGCTAGATAGTGAGCGGATACCTGAAGCAGCATTGATGGCACGATCTTACCTACCCAGCAAGGTGTCAGAGATAGTTTCAATCTGGAGAAATGACCTGAACAAGGTTAATAAAAGAGCTGCAGAATCTTTGGCAGATCCCAAAGAATATCCTAATTTGTTTGAGGACTGGGAGATTTCTCTTTCCCTTGAGTCTAACCGCACTGAAACCAGTGGCAATTATCAACCAGCTGAACAGTACCCAGTCTATGCTTCTGAGAAGCCAGCTAGCAGCCTTGTTGAATTATTCAAAAGCATGCACATTGATGAAGAAGAAGCAGCCCCAGTTGAAAATGGAGATCTGGATCATGAGGAGGTAGAGGAGAATGAAGAAGATCCAGGTGAAGAACATACCATTGAGGAGGATTCAGACTCCATGAATGGTGTTGTTCTTGTGAATGGCACCCATGAGGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

898

Amino Acids

101.78

Weight (kDa)

4.92

Isoelectric Point (pI)

39.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	10 - 155	5.8e-14	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	12 - 152	8.4e-11	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	13 - 130	1.1e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	14 - 171	2.9e-24	WDR5 beta-propeller domain
WDR55	PF24796	16 - 198	8.9e-15	WDR55
WD40_Prp19	PF24814	16 - 191	1.3e-20	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	21 - 191	3e-23	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	21 - 174	2.3e-09	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	34 - 223	4.6e-20	CDC20/Fizzy WD40 domain
Beta-prop_EIPR1	PF23609	53 - 170	4.3e-08	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	58 - 131	1.9e-10	WDHD1 first WD40 domain
WD40_Gbeta	PF25391	68 - 216	4e-14	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	68 - 172	4.8e-08	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	69 - 260	5.3e-13	TEP-1 second beta-propeller
Beta-prop_EML	PF23409	76 - 265	5e-13	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	91 - 127	3.4e-08	WD domain, G-beta repeat
Beta-prop_RIG_2nd	PF23775	132 - 246	5e-06	RIG second beta-propeller
WD40	PF00400	133 - 171	9.8e-07	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	135 - 295	2.9e-27	WDR3 first beta-propeller domain
EIF3I	PF24805	137 - 229	5.5e-06	EIF3I
Beta-prop_WDR5	PF25175	139 - 288	1.1e-31	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	140 - 260	3.7e-14	WDR3 second beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	163 - 256	3.6e-06	WDR36/Utp21 first beta-propeller
WD40	PF00400	177 - 215	2.1e-06	WD domain, G-beta repeat
WDR55	PF24796	182 - 296	3.1e-11	WDR55
WD40_Prp19	PF24814	185 - 265	3.4e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	186 - 295	2.9e-15	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	196 - 265	1.1e-06	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	198 - 322	3.1e-12	WDHD1 first WD40 domain
Beta-prop_WDR75_1st	PF23869	198 - 261	2.1e-06	WD repeat-containing protein 75 first beta-propeller
WD40_CDC20-Fz	PF24807	198 - 270	1.5e-08	CDC20/Fizzy WD40 domain
Beta-prop_EML_2	PF23414	199 - 296	4.6e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	200 - 260	4.6e-09	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	201 - 258	2.5e-07	WDR90/POC16, second beta-propeller
Beta-prop_SCAP	PF24017	202 - 259	9.7e-06	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 261	7.2e-07	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	220 - 256	2e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 298	5.6e-06	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	6e-87	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	2.4e-81	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	731, 1611
Acc16I	TGCGCA	1 cut(s)	36
AccB1I	GGYRCC	1 cut(s)	2675
AccBSI	CCGCTC	1 cut(s)	2251
AccI	GTMKAC	1 cut(s)	249
AccII	CGCG	1 cut(s)	246
AciI	CCGC	5 cut(s)	1173, 1422, 1627, 2251, 2434
AcoI	YGGCCR	2 cut(s)	1032, 1434
AcsI	RAATTY	4 cut(s)	191, 1072, 1239, 1934
AcuI	CTGAAG	2 cut(s)	2082, 2280
AcyI	GRCGYC	1 cut(s)	546
AdeI	CACNNNGTG	1 cut(s)	2000
AfaI	GTAC	7 cut(s)	104, 403, 602, 1127, 1688, 1784, 2474
AfiI	CCNNNNNNNGG	5 cut(s)	783, 1095, 1626, 1876, 2338
AflIII	ACRYGT	3 cut(s)	234, 244, 817
AjiI	CACGTC	1 cut(s)	851
AjnI	CCWGG	3 cut(s)	1257, 1622, 2611
AloI	GAACNNNNNNTCC	2 cut(s)	72, 104
AluBI	AGCT	9 cut(s)	1549, 1773, 1954, 2020, 2033, 2233, 2354, 2465, 2501
AluI	AGCT	9 cut(s)	1549, 1773, 1954, 2020, 2033, 2233, 2354, 2465, 2501
Alw21I	GWGCWC	1 cut(s)	1551
Alw26I	GTCTC	2 cut(s)	169, 1492
AlwNI	CAGNNNCTG	2 cut(s)	617, 1925
AoxI	GGCC	6 cut(s)	948, 1032, 1434, 1511, 1679, 1866
ApeKI	GCWGC	7 cut(s)	221, 224, 2017, 2264, 2354, 2505, 2552
ApoI	RAATTY	4 cut(s)	191, 1072, 1239, 1934
ArsI	GACNNNNNNTTYG	2 cut(s)	1919, 1951
AspLEI	GCGC	2 cut(s)	37, 292
AspS9I	GGNCC	5 cut(s)	111, 948, 1675, 1680, 1867
AsuHPI	GGTGA	4 cut(s)	602, 1436, 1666, 2627
AsuNHI	GCTAGC	2 cut(s)	917, 2501
AvaII	GGWCC	2 cut(s)	111, 1675
BalI	TGGCCA	1 cut(s)	1436
BamHI	GGATCC	1 cut(s)	87
BanI	GGYRCC	1 cut(s)	2675
BanII	GRGCYC	1 cut(s)	1551
BauI	CACGAG	1 cut(s)	1884
BbsI	GAAGAC	1 cut(s)	274
Bbv12I	GWGCWC	1 cut(s)	1551
BbvI	GCAGC	7 cut(s)	208, 236, 2029, 2276, 2341, 2517, 2564
BccI	CCATC	5 cut(s)	330, 743, 1007, 1112, 2266
BceAI	ACGGC	3 cut(s)	583, 1019, 1155
BciT130I	CCWGG	3 cut(s)	1259, 1624, 2613
BciVI	GTATCC	2 cut(s)	1748, 2247
BclI	TGATCA	1 cut(s)	622
BcoDI	GTCTC	2 cut(s)	169, 1492
BfaI	CTAG	7 cut(s)	918, 1145, 1443, 1526, 1964, 2240, 2502
BfmI	CTRYAG	4 cut(s)	222, 699, 1917, 2355
BfuI	GTATCC	2 cut(s)	1748, 2247
BglI	GCCNNNNNGGC	1 cut(s)	10
BglII	AGATCT	1 cut(s)	2572
BisI	GCNGC	8 cut(s)	222, 225, 1628, 2018, 2265, 2355, 2506, 2553
BlsI	GCNGC	8 cut(s)	223, 226, 1629, 2019, 2266, 2356, 2507, 2554
Bme1390I	CCNGG	3 cut(s)	1259, 1624, 2613
Bme18I	GGWCC	2 cut(s)	111, 1675
BmgBI	CACGTC	1 cut(s)	851
BmgT120I	GGNCC	5 cut(s)	111, 948, 1675, 1680, 1867
BmiI	GGNNCC	6 cut(s)	89, 112, 880, 1051, 1171, 2677
BmrFI	CCNGG	3 cut(s)	1259, 1624, 2613
BmrI	ACTGGG	5 cut(s)	391, 1366, 2413, 2471, 2552
BmsI	GCATC	4 cut(s)	1528, 1891, 2116, 2235
BmtI	GCTAGC	2 cut(s)	921, 2505
BmuI	ACTGGG	5 cut(s)	391, 1366, 2413, 2471, 2552
BpiI	GAAGAC	1 cut(s)	274
BpmI	CTGGAG	1 cut(s)	2341
BpuEI	CTTGAG	2 cut(s)	2115, 2444
BsaBI	GATNNNNATC	3 cut(s)	69, 1062, 2577
BsaHI	GRCGYC	1 cut(s)	546
BsaJI	CCNNGG	2 cut(s)	83, 264
Bsc4I	CCNNNNNNNGG	5 cut(s)	783, 1095, 1626, 1876, 2338
Bse118I	RCCGGY	1 cut(s)	1677
Bse3DI	GCAATG	3 cut(s)	215, 1329, 1437
Bse8I	GATNNNNATC	3 cut(s)	69, 1062, 2577
BseBI	CCWGG	3 cut(s)	1259, 1624, 2613
BseDI	CCNNGG	2 cut(s)	83, 264
BseGI	GGATG	7 cut(s)	70, 322, 402, 754, 1896, 1906, 2226
BseJI	GATNNNNATC	3 cut(s)	69, 1062, 2577
BseLI	CCNNNNNNNGG	5 cut(s)	783, 1095, 1626, 1876, 2338
BseMI	GCAATG	3 cut(s)	215, 1329, 1437
BseMII	CTCAG	6 cut(s)	22, 120, 419, 735, 1783, 2481
BseRI	GAGGAG	5 cut(s)	1541, 1609, 2600, 2609, 2648
BseXI	GCAGC	7 cut(s)	208, 236, 2029, 2276, 2341, 2517, 2564
BseYI	CCCAGC	4 cut(s)	1954, 2020, 2206, 2291
Bsh1236I	CGCG	1 cut(s)	246
BshFI	GGCC	6 cut(s)	950, 1034, 1436, 1513, 1681, 1868
BshNI	GGYRCC	1 cut(s)	2675
BsiHKAI	GWGCWC	1 cut(s)	1551
BsiSI	CCGG	1 cut(s)	1678
BslFI	GGGAC	4 cut(s)	124, 392, 656, 1324
BslI	CCNNNNNNNGG	5 cut(s)	783, 1095, 1626, 1876, 2338
BsmAI	GTCTC	2 cut(s)	169, 1492
BsmFI	GGGAC	4 cut(s)	124, 392, 656, 1324
BsmI	GAATGC	4 cut(s)	421, 769, 2186, 2228
BsnI	GGCC	6 cut(s)	950, 1034, 1436, 1513, 1681, 1868
Bsp1286I	GDGCHC	1 cut(s)	1551
Bsp19I	CCATGG	2 cut(s)	83, 264
BspACI	CCGC	5 cut(s)	1173, 1422, 1627, 2251, 2434
BspANI	GGCC	6 cut(s)	950, 1034, 1436, 1513, 1681, 1868
BspCNI	CTCAG	6 cut(s)	21, 119, 418, 736, 1782, 2482
BspFNI	CGCG	1 cut(s)	246
BspHI	TCATGA	1 cut(s)	2581
BspLI	GGNNCC	6 cut(s)	89, 112, 880, 1051, 1171, 2677
BspMAI	CTGCAG	3 cut(s)	226, 703, 2359
BspOI	GCTAGC	2 cut(s)	921, 2505
BspT107I	GGYRCC	1 cut(s)	2675
BsrBI	CCGCTC	1 cut(s)	2251
BsrDI	GCAATG	3 cut(s)	215, 1329, 1437
BsrFI	RCCGGY	1 cut(s)	1677
BssAI	RCCGGY	1 cut(s)	1677
BssECI	CCNNGG	2 cut(s)	83, 264
BssNAI	GTATAC	1 cut(s)	250
BssNI	GRCGYC	1 cut(s)	546
BssSI	CACGAG	1 cut(s)	1884
BssT1I	CCWWGG	2 cut(s)	83, 264
Bst1107I	GTATAC	1 cut(s)	250
Bst2BI	CACGAG	1 cut(s)	1884
Bst2UI	CCWGG	3 cut(s)	1259, 1624, 2613
Bst4CI	ACNGT	9 cut(s)	115, 375, 499, 757, 973, 1601, 1640, 1862, 2472
Bst6I	CTCTTC	2 cut(s)	1495, 2682
BstACI	GRCGYC	1 cut(s)	546
BstC8I	GCNNGC	6 cut(s)	521, 919, 1818, 2499, 2503, 2531
BstDEI	CTNAG	7 cut(s)	8, 106, 150, 405, 744, 1769, 2490
BstDSI	CCRYGG	2 cut(s)	83, 264
BstEII	GGTNACC	2 cut(s)	1023, 1654
BstENI	CCTNNNNNAGG	1 cut(s)	2336
BstF5I	GGATG	7 cut(s)	70, 322, 402, 754, 1896, 1906, 2226
BstFNI	CGCG	1 cut(s)	246
BstHHI	GCGC	2 cut(s)	37, 292
BstMAI	GTCTC	2 cut(s)	169, 1492
BstMWI	GCNNNNNNNGC	4 cut(s)	10, 1991, 2156, 2273
BstNI	CCWGG	3 cut(s)	1259, 1624, 2613
BstNSI	RCATGY	3 cut(s)	238, 364, 2533
BstPI	GGTNACC	2 cut(s)	1023, 1654
BstSCI	CCNGG	3 cut(s)	1257, 1622, 2611
BstSFI	CTRYAG	4 cut(s)	222, 699, 1917, 2355
BstUI	CGCG	1 cut(s)	246
BstV1I	GCAGC	7 cut(s)	208, 236, 2029, 2276, 2341, 2517, 2564
BstV2I	GAAGAC	1 cut(s)	274
BstX2I	RGATCY	9 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572, 2608
BstYI	RGATCY	9 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572, 2608
BstZ17I	GTATAC	1 cut(s)	250
BsuI	GTATCC	2 cut(s)	1748, 2247
BsuRI	GGCC	6 cut(s)	950, 1034, 1436, 1513, 1681, 1868
BtgI	CCRYGG	2 cut(s)	83, 264
BtrI	CACGTC	1 cut(s)	851
BtsCI	GGATG	7 cut(s)	70, 322, 402, 754, 1896, 1906, 2226
BtsIMutI	CAGTG	7 cut(s)	294, 495, 582, 1867, 1904, 2436, 2452
Cac8I	GCNNGC	6 cut(s)	521, 919, 1818, 2499, 2503, 2531
CaiI	CAGNNNCTG	2 cut(s)	617, 1925
CciI	TCATGA	1 cut(s)	2581
CfoI	GCGC	2 cut(s)	37, 292
Cfr10I	RCCGGY	1 cut(s)	1677
Cfr13I	GGNCC	5 cut(s)	111, 948, 1675, 1680, 1867
CseI	GACGC	1 cut(s)	554
Csp6I	GTAC	7 cut(s)	103, 402, 601, 1126, 1687, 1783, 2473
CviQI	GTAC	7 cut(s)	103, 402, 601, 1126, 1687, 1783, 2473
DdeI	CTNAG	7 cut(s)	8, 106, 150, 405, 744, 1769, 2490
DraIII	CACNNNGTG	1 cut(s)	2000
EaeI	YGGCCR	2 cut(s)	1032, 1434
Eam1104I	CTCTTC	2 cut(s)	1495, 2682
EarI	CTCTTC	2 cut(s)	1495, 2682
Ecl136II	GAGCTC	1 cut(s)	1549
Eco130I	CCWWGG	2 cut(s)	83, 264
Eco24I	GRGCYC	1 cut(s)	1551
Eco32I	GATATC	1 cut(s)	1700
Eco47I	GGWCC	2 cut(s)	111, 1675
Eco53kI	GAGCTC	1 cut(s)	1549
Eco57I	CTGAAG	2 cut(s)	2082, 2280
Eco91I	GGTNACC	2 cut(s)	1023, 1654
EcoICRI	GAGCTC	1 cut(s)	1549
EcoNI	CCTNNNNNAGG	1 cut(s)	2336
EcoO65I	GGTNACC	2 cut(s)	1023, 1654
EcoRII	CCWGG	3 cut(s)	1257, 1622, 2611
EcoRV	GATATC	1 cut(s)	1700
EcoT14I	CCWWGG	2 cut(s)	83, 264
EcoT22I	ATGCAT	1 cut(s)	2228
EcoT38I	GRGCYC	1 cut(s)	1551
ErhI	CCWWGG	2 cut(s)	83, 264
FalI	AAGNNNNNCTT	2 cut(s)	587, 619
FaqI	GGGAC	4 cut(s)	124, 392, 656, 1324
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	1 cut(s)	249
Fnu4HI	GCNGC	8 cut(s)	222, 225, 1628, 2018, 2265, 2355, 2506, 2553
FokI	GGATG	7 cut(s)	57, 309, 409, 761, 1903, 1913, 2213
FriOI	GRGCYC	1 cut(s)	1551
Fsp4HI	GCNGC	8 cut(s)	222, 225, 1628, 2018, 2265, 2355, 2506, 2553
FspBI	CTAG	7 cut(s)	918, 1145, 1443, 1526, 1964, 2240, 2502
FspI	TGCGCA	1 cut(s)	36
GlaI	GCGC	2 cut(s)	36, 291
GluI	GCNGC	8 cut(s)	222, 225, 1628, 2018, 2265, 2355, 2506, 2553
GsaI	CCCAGC	4 cut(s)	1958, 2024, 2210, 2295
GsuI	CTGGAG	1 cut(s)	2341
HaeIII	GGCC	6 cut(s)	950, 1034, 1436, 1513, 1681, 1868
HapII	CCGG	1 cut(s)	1678
HgaI	GACGC	1 cut(s)	554
HhaI	GCGC	2 cut(s)	37, 292
Hin1I	GRCGYC	1 cut(s)	546
Hin6I	GCGC	2 cut(s)	35, 290
HinP1I	GCGC	2 cut(s)	35, 290
HinfI	GANTC	8 cut(s)	511, 1448, 1880, 2003, 2360, 2426, 2639, 2645
HpaII	CCGG	1 cut(s)	1678
HphI	GGTGA	4 cut(s)	602, 1436, 1666, 2627
Hpy166II	GTNNAC	3 cut(s)	242, 250, 402
Hpy188III	TCNNGA	6 cut(s)	124, 1925, 1964, 2320, 2576, 2582
Hpy8I	GTNNAC	3 cut(s)	242, 250, 402
Hpy99I	CGWCG	1 cut(s)	855
HpyAV	CCTTC	6 cut(s)	410, 671, 869, 1033, 1089, 1503
HpyCH4III	ACNGT	9 cut(s)	115, 375, 499, 757, 973, 1601, 1640, 1862, 2472
HpyCH4IV	ACGT	2 cut(s)	817, 850
HpyF10VI	GCNNNNNNNGC	4 cut(s)	10, 1991, 2156, 2273
HpyF3I	CTNAG	7 cut(s)	8, 106, 150, 405, 744, 1769, 2490
HpySE526I	ACGT	2 cut(s)	817, 850
Hsp92I	GRCGYC	1 cut(s)	546
HspAI	GCGC	2 cut(s)	35, 290
Ksp22I	TGATCA	1 cut(s)	622
LmnI	GCTCC	3 cut(s)	992, 1175, 1554
Lsp1109I	GCAGC	7 cut(s)	208, 236, 2029, 2276, 2341, 2517, 2564
LweI	GCATC	4 cut(s)	1528, 1891, 2116, 2235
MaeI	CTAG	7 cut(s)	918, 1145, 1443, 1526, 1964, 2240, 2502
MaeII	ACGT	2 cut(s)	817, 850
MaeIII	GTNAC	6 cut(s)	135, 196, 442, 1023, 1654, 2100
MbiI	CCGCTC	1 cut(s)	2251
MflI	RGATCY	9 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572, 2608
MhlI	GDGCHC	1 cut(s)	1551
MlsI	TGGCCA	1 cut(s)	1436
MluI	ACGCGT	1 cut(s)	244
MluNI	TGGCCA	1 cut(s)	1436
MlyI	GAGTC	2 cut(s)	2435, 2639
MmeI	TCCRAC	3 cut(s)	98, 833, 1875
Mox20I	TGGCCA	1 cut(s)	1436
Mph1103I	ATGCAT	1 cut(s)	2228
MscI	TGGCCA	1 cut(s)	1436
MseI	TTAA	5 cut(s)	21, 276, 450, 605, 2343
MslI	CAYNNNNRTG	4 cut(s)	440, 1302, 1860, 2538
Msp20I	TGGCCA	1 cut(s)	1436
MspA1I	CMGCKG	4 cut(s)	1954, 2020, 2233, 2465
MspI	CCGG	1 cut(s)	1678
MspR9I	CCNGG	3 cut(s)	1259, 1624, 2613
Mva1269I	GAATGC	4 cut(s)	421, 769, 2186, 2228
MvaI	CCWGG	3 cut(s)	1259, 1624, 2613
MvnI	CGCG	1 cut(s)	246
MwoI	GCNNNNNNNGC	4 cut(s)	10, 1991, 2156, 2273
NcoI	CCATGG	2 cut(s)	83, 264
NdeI	CATATG	1 cut(s)	337
NheI	GCTAGC	2 cut(s)	917, 2501
NlaIV	GGNNCC	6 cut(s)	89, 112, 880, 1051, 1171, 2677
NmuCI	GTSAC	3 cut(s)	135, 442, 1654
NsbI	TGCGCA	1 cut(s)	36
NsiI	ATGCAT	1 cut(s)	2228
NspI	RCATGY	3 cut(s)	238, 364, 2533
PaeI	GCATGC	1 cut(s)	2533
PagI	TCATGA	1 cut(s)	2581
PciI	ACATGT	1 cut(s)	234
PctI	GAATGC	4 cut(s)	421, 769, 2186, 2228
PfeI	GAWTC	6 cut(s)	511, 1448, 1880, 2003, 2360, 2639
PflFI	GACNNNGTC	1 cut(s)	1340
PfoI	TCCNGGA	1 cut(s)	1257
PkrI	GCNGC	8 cut(s)	223, 226, 1629, 2019, 2266, 2356, 2507, 2554
PleI	GAGTC	2 cut(s)	2434, 2639
PpsI	GAGTC	2 cut(s)	2434, 2639
PscI	ACATGT	1 cut(s)	234
PsiI	TTATAA	2 cut(s)	731, 1611
Psp124BI	GAGCTC	1 cut(s)	1551
Psp6I	CCWGG	3 cut(s)	1257, 1622, 2611
PspEI	GGTNACC	2 cut(s)	1023, 1654
PspFI	CCCAGC	4 cut(s)	1954, 2020, 2206, 2291
PspGI	CCWGG	3 cut(s)	1257, 1622, 2611
PspN4I	GGNNCC	6 cut(s)	89, 112, 880, 1051, 1171, 2677
PspPI	GGNCC	5 cut(s)	111, 948, 1675, 1680, 1867
PstI	CTGCAG	3 cut(s)	226, 703, 2359
PstNI	CAGNNNCTG	2 cut(s)	617, 1925
PsuI	RGATCY	9 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572, 2608
PsyI	GACNNNGTC	1 cut(s)	1340
PvuII	CAGCTG	4 cut(s)	1954, 2020, 2233, 2465
RsaI	GTAC	7 cut(s)	104, 403, 602, 1127, 1688, 1784, 2474
RsaNI	GTAC	7 cut(s)	103, 402, 601, 1126, 1687, 1783, 2473
RseI	CAYNNNNRTG	4 cut(s)	440, 1302, 1860, 2538
SacI	GAGCTC	1 cut(s)	1551
SaqAI	TTAA	5 cut(s)	21, 276, 450, 605, 2343
SatI	GCNGC	8 cut(s)	222, 225, 1628, 2018, 2265, 2355, 2506, 2553
Sau96I	GGNCC	5 cut(s)	111, 948, 1675, 1680, 1867
SchI	GAGTC	2 cut(s)	2435, 2639
ScrFI	CCNGG	3 cut(s)	1259, 1624, 2613
SduI	GDGCHC	1 cut(s)	1551
SfaNI	GCATC	4 cut(s)	1528, 1891, 2116, 2235
SfcI	CTRYAG	4 cut(s)	222, 699, 1917, 2355
SinI	GGWCC	2 cut(s)	111, 1675
SmiMI	CAYNNNNRTG	4 cut(s)	440, 1302, 1860, 2538
SmlI	CTYRAG	2 cut(s)	2130, 2423
SmoI	CTYRAG	2 cut(s)	2130, 2423
SphI	GCATGC	1 cut(s)	2533
SsiI	CCGC	5 cut(s)	1173, 1422, 1627, 2251, 2434
SspI	AATATT	1 cut(s)	1273
SspMI	CTAG	7 cut(s)	918, 1145, 1443, 1526, 1964, 2240, 2502
SstI	GAGCTC	1 cut(s)	1551
StyD4I	CCNGG	3 cut(s)	1257, 1622, 2611
StyI	CCWWGG	2 cut(s)	83, 264
TaaI	ACNGT	9 cut(s)	115, 375, 499, 757, 973, 1601, 1640, 1862, 2472
TaiI	ACGT	2 cut(s)	820, 853
TaqI	TCGA	4 cut(s)	285, 489, 773, 1276
TaqII	GACCGA	1 cut(s)	171
TatI	WGTACW	5 cut(s)	102, 401, 1125, 1686, 1782
TauI	GCSGC	1 cut(s)	1630
TfiI	GAWTC	6 cut(s)	511, 1448, 1880, 2003, 2360, 2639
Tru1I	TTAA	5 cut(s)	21, 276, 450, 605, 2343
Tru9I	TTAA	5 cut(s)	21, 276, 450, 605, 2343
TscAI	CASTG	7 cut(s)	301, 502, 589, 1867, 1911, 2443, 2452
TseFI	GTSAC	3 cut(s)	135, 442, 1654
TseI	GCWGC	7 cut(s)	221, 224, 2017, 2264, 2354, 2505, 2552
Tsp45I	GTSAC	3 cut(s)	135, 442, 1654
TspDTI	ATGAA	9 cut(s)	854, 975, 1440, 1536, 1658, 2191, 2556, 2616, 2666
TspGWI	ACGGA	1 cut(s)	1215
TspRI	CASTG	7 cut(s)	301, 502, 589, 1867, 1911, 2443, 2452
Tth111I	GACNNNGTC	1 cut(s)	1340
VpaK11BI	GGWCC	2 cut(s)	111, 1675
XagI	CCTNNNNNAGG	1 cut(s)	2336
XapI	RAATTY	4 cut(s)	191, 1072, 1239, 1934
XbaI	TCTAGA	1 cut(s)	1963
XceI	RCATGY	3 cut(s)	238, 364, 2533
XcmI	CCANNNNNNNNNTGG	2 cut(s)	81, 1690
XmiI	GTMKAC	1 cut(s)	249
XspI	CTAG	7 cut(s)	918, 1145, 1443, 1526, 1964, 2240, 2502
Zsp2I	ATGCAT	1 cut(s)	2228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.