Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Forward (+)

14845849 .. 14856156

Gene Structure 10308 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1188300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2718 bp

ATGCCTCTCAGGCTCGATATCAAGCGGAAATTTGTGCAAAGAACCGAGAGAGTTAAGTCTTTGGATCTACATCCATCTGAGCCATGGATCCTTGCGAGTCTGTATTCGGGAACCGTATACATCTTCAATTACCAGTCACAGAGCATGGCAAAGTCTTTTGAAGTCACTGATTTGCCAGTTCGGTCAGCAAAGTTTGTAGTGCGTAAGCAGTGGGTAGTTGCTGGAGCTGATGACATGTTTATTCGTGTATACAATTATAATACAATGGATAAAGTTAAAGTCTTTGAAGCACACTCGGACTACATTAGATGTGTGGCTGTCCATCCAACACTTCCATATGTTTTGTCATCATCCGATGACATGCTTATCAAACTGTGGGATTGGGAAAAAGGTTGGATGTGTACTCAGATATTTGAAGGACATTCTCACTATGTGATGCAAGTGACCTTTAACCCTAAAGACACCAACACTTTTGCAAGTGCTTCCTTGGATCATACTGTGAAGATCTGGAATCTTGTCTCCCCTGACCCAAATTTTACATTGGATGCTCACTCGAAAGGGGTAAATTGTGTTGATTACTTCACTGGCGGTGATAAACCATACTTAATCACAGGTTCAGATGATCATACAGCAAAGGTGTGGGACTATCAGACAAAAAGCTGTGTTCAGACACTTGATGGACATACTCACAATGTATCTGCAGTTTGCTTTCATCCGGAACTTCCAGTTATAATTACTGGTTCAGAGGATGGAACTGTTAGAATATGGCACTCGACCACTTATCGGCTTGAAAACACATTGAATTATGGACTTGAACGTGTTTGGGCTTTTGGGTACATGAAAGGTTCACGCCGGATTGTAATTGGTTATGATGAAGGAGCCATAATGGTTAAAATTGGTCGAGAAGTACCAGTTGCTAGCATGGATAGTGGTGGAAAAATTATTTGGGCAAAGCATAATGAGATTCAAACTGTGAATATCAAAAGTGTTGGAGCAGATTTTGAGGCTACAGATGGAGAAAGATTGCCTTTGGCCGTGAAGGAGTTGGGAACCTGTGACCTTTATCCTCAAAGTTTGAAGCACAACCCCAATGGAAGGTTTGTTGTTGTTTGTGGAGATGGCGAGTACATAATATACACAGCTTTAGCATGGAGAAATAGATCCTTTGGCTCAGCATTGGAATTTGTTTGGTCCTCGGATGGAGAATGTGCTGTTAGGGAAACTACATCAAGGATAAAAGTTTATAGCAAAACGTTCCAGGAAAAAAAGAATGTTCGACCAACATTTTCTGTAGAACATATTTATGGAGGGGTCTTACTGGCAATGCGCTCAAATGACTTCATATGCTTCTATGACTGGGCGGAATGCAGATTGATTAGACGTATTGATGTCAATGTTAAGAATGTTTATTGGGCTGATAGTGGCGATTTGGTGGCAATCACAAGTGATTCATCATTCTACATTCTAAAATACGATAGGGATGTAGTCTCATCCTATTTTGACAGTGGAAGGCCAGTTGATGAACTAGGTGTGGAGGATGCTTTTGAGCTCCTCTATGAAATAAATGAGCGTGTCAGAACTGGATTATGGGTCGGGGACTGTTTCGTTTACAATAACGCTTCTTGGCGACTTAACTATTGTGTTGGTGGCGAGGTGACCACAATGTTTCACCTGGACCGGCCCATGTACTTGCTGGGATATCTTGCCAATCAAAGTCGTCTCTTTCTAATTGATAAAGAGTTTAATGTCATTGGATACACCTTGCTTCTCAGCTTGATCGAGTACAAAACACTTGTTATACGTGGAGATATAGAGCGTGCGAAGCAAATCTTTCCAACAATTCCTCCAGAGCAACATAATAGTGTGGCTCGATTTTTGGAATCTCGAGGTATGTTGGAGGATGCACTGGAAGTGGCAACAGATGCTGACTACAAATTTGATCTTGCTGTACAGCTGGGTAGACTTGAGATTGCAAAGGAAATTGCTACAGGAGACCAAAGTGAATCCAAATGGAAGCGGCTGGGAGAATTAGCTATGTCCACTGGGAAGCTTGATTTGGCTGAAGATTGTCTATCAAATGGAATGGACCTTTCTGGCTTGTTGCTTCTCTATTCTTCTCTTGGAGATGCCCAAGGAATATCAAAACTTGCATCCCTTGCCAAAGAACAAGGAAAGAATAATGTTGCATTCCTTTGTTTATTTATGCTGGGTAAATTAGAAGAATGCATACAGCTGTTGTTGGAAAGTGAAAGGATACCTGAAGCAGCTTTAATGGCACGATCTTACCTACCAAGCAAGGTCTCAGAGATAGTTTCAATTTGGAGAAATGACCTGAACAAGGTCAATAAAAAAGCTGCCGAATCATTGGCCGATCCACAAGAGTATCCTAATTTGTTCGAGGACTGGCAGGTTTCTCTTGCCCTTGAGTCTAAAGACGCAGAAAATAGAGGCATTTATCCTCCTGCTGAACAATACCCAGTCTATGCTGAGAAGCCAACCACCAACCTAGTGGAAAGGTTCAGAAGCATGCAAATTGATGAAGAAGCGACAATCGAAAATGGAGATCTGGATCATGAGGATGTGCAGGAGAATGGAGAAGATCAAGGTGAAGGAGAGGCTGTTGAGGAGGATGCTGACTCCATGAACGGTGTTGTTCTTGTAAATGGAAACCAGGATGAAGAGTCGGATAAAAATGATGAAACTACTTCATCACCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

906

Amino Acids

102.39

Weight (kDa)

4.9

Isoelectric Point (pI)

36.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	9 - 151	3.8e-12	WDR3 second beta-propeller domain
Beta-prop_EML	PF23409	10 - 165	1.9e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_EML_2	PF23414	12 - 130	4.7e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	12 - 155	1.2e-15	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	14 - 153	5.8e-19	WDR5 beta-propeller domain
WDR55	PF24796	15 - 199	2.8e-15	WDR55
WD40_Prp19	PF24814	16 - 185	6.6e-20	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	20 - 192	2.3e-22	THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	28 - 174	1.4e-10	WDR36/Utp21 second beta-propeller domain
WD40_CDC20-Fz	PF24807	31 - 193	2.2e-16	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	58 - 131	5.9e-11	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	59 - 194	9.7e-27	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	60 - 166	7.4e-07	MABP1/WDR62 second WD40 domain
WD40_Gbeta	PF25391	63 - 216	2.4e-14	G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd	PF25047	68 - 260	4.6e-14	TEP-1 second beta-propeller
Beta-prop_EIPR1	PF23609	81 - 170	3.5e-07	EIPR1 beta-propeller
WD40	PF00400	91 - 127	5.3e-08	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	104 - 230	6e-06	Echinoderm microtubule-associated protein second beta-propeller
WD40	PF00400	133 - 171	1.1e-06	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	135 - 295	1.2e-27	WDR3 first beta-propeller domain
EIF3I	PF24805	137 - 228	1.4e-06	EIF3I
Beta-prop_RIG_2nd	PF23775	137 - 245	2.3e-06	RIG second beta-propeller
Beta-prop_WDR3_2nd	PF25172	140 - 260	2.8e-14	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	154 - 288	4.7e-29	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	161 - 256	1.4e-06	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	164 - 241	6.7e-07	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	177 - 215	4e-07	WD domain, G-beta repeat
WDR55	PF24796	181 - 296	2.4e-11	WDR55
WD40_Prp19	PF24814	183 - 265	2.4e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	184 - 292	2.4e-15	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	197 - 265	2.1e-06	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	198 - 270	2e-08	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	198 - 307	1.1e-11	WDHD1 first WD40 domain
Beta-prop_WDR75_1st	PF23869	198 - 261	4.1e-06	WD repeat-containing protein 75 first beta-propeller
Beta-prop_EML_2	PF23414	199 - 296	6.1e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	201 - 258	3.7e-07	WDR90/POC16, second beta-propeller
WD40_Gbeta	PF25391	201 - 260	5.7e-09	G protein beta WD-40 repeat protein
Beta-prop_SCAP	PF24017	202 - 259	5.2e-06	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 261	1.2e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	220 - 256	4e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 297	9.9e-06	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	7e-86	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	1.5e-78	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	258, 731
Acc36I	ACCTGC	1 cut(s)	2398
AccI	GTMKAC	3 cut(s)	117, 249, 1960
AccIII	TCCGGA	1 cut(s)	715
AciI	CCGC	4 cut(s)	25, 588, 1361, 2017
AclI	AACGTT	1 cut(s)	1253
AclWI	GGATC	7 cut(s)	72, 82, 95, 498, 1155, 2366, 2577
AcoI	YGGCCR	2 cut(s)	1032, 2367
AcsI	RAATTY	4 cut(s)	29, 532, 1181, 1934
AcuI	CTGAAG	2 cut(s)	2082, 2280
AdeI	CACNNNGTG	1 cut(s)	433
AfaI	GTAC	7 cut(s)	403, 836, 909, 1127, 1688, 1784, 1950
AfiI	CCNNNNNNNGG	3 cut(s)	783, 1095, 2338
AflIII	ACRYGT	2 cut(s)	234, 817
AjnI	CCWGG	3 cut(s)	1257, 1671, 2670
AjuI	GAANNNNNNNTTGG	4 cut(s)	928, 960, 2039, 2071
Alw21I	GWGCWC	1 cut(s)	1551
Alw26I	GTCTC	5 cut(s)	523, 1492, 1724, 1986, 2305
AlwI	GGATC	7 cut(s)	72, 82, 95, 498, 1155, 2366, 2577
AlwNI	CAGNNNCTG	1 cut(s)	1925
Ama87I	CYCGRG	1 cut(s)	1884
Aor13HI	TCCGGA	1 cut(s)	715
AoxI	GGCC	4 cut(s)	1032, 1511, 1679, 2367
ApeKI	GCWGC	2 cut(s)	2264, 2354
ApoI	RAATTY	4 cut(s)	29, 532, 1181, 1934
ArsI	GACNNNNNNTTYG	2 cut(s)	1919, 1951
AspLEI	GCGC	1 cut(s)	1329
AspS9I	GGNCC	4 cut(s)	1191, 1675, 1680, 2086
AsuHPI	GGTGA	5 cut(s)	602, 1661, 1666, 2618, 2703
AsuNHI	GCTAGC	1 cut(s)	917
AvaI	CYCGRG	1 cut(s)	1884
AvaII	GGWCC	3 cut(s)	1191, 1675, 2086
BaeI	ACNNNNGTAYC	2 cut(s)	2366, 2399
BamHI	GGATCC	1 cut(s)	87
BanII	GRGCYC	1 cut(s)	1551
BarI	GAAGNNNNNNTAC	2 cut(s)	2211, 2243
Bbv12I	GWGCWC	1 cut(s)	1551
BbvI	GCAGC	2 cut(s)	2276, 2341
BccI	CCATC	7 cut(s)	82, 330, 671, 743, 1007, 1112, 1193
BceAI	ACGGC	1 cut(s)	1019
BciT130I	CCWGG	3 cut(s)	1259, 1673, 2672
BciVI	GTATCC	3 cut(s)	1748, 2247, 2394
BclI	TGATCA	1 cut(s)	622
BcoDI	GTCTC	5 cut(s)	523, 1492, 1724, 1986, 2305
BfaI	CTAG	3 cut(s)	918, 1526, 2507
BfmI	CTRYAG	4 cut(s)	699, 1008, 1290, 1986
BfuAI	ACCTGC	1 cut(s)	2398
BfuI	GTATCC	3 cut(s)	1748, 2247, 2394
BglI	GCCNNNNNGGC	1 cut(s)	10
BglII	AGATCT	2 cut(s)	504, 2563
BisI	GCNGC	3 cut(s)	2018, 2265, 2355
BlpI	GCTNAGC	1 cut(s)	1171
BlsI	GCNGC	3 cut(s)	2019, 2266, 2356
Bme1390I	CCNGG	3 cut(s)	1259, 1673, 2672
Bme18I	GGWCC	3 cut(s)	1191, 1675, 2086
BmeT110I	CYCGRG	1 cut(s)	1884
BmgT120I	GGNCC	4 cut(s)	1191, 1675, 1680, 2086
BmiI	GGNNCC	4 cut(s)	89, 112, 880, 1051
BmrFI	CCNGG	3 cut(s)	1259, 1673, 2672
BmrI	ACTGGG	3 cut(s)	1366, 2052, 2471
BmsI	GCATC	8 cut(s)	426, 535, 1528, 1891, 1912, 2116, 2159, 2620
BmtI	GCTAGC	1 cut(s)	921
BmuI	ACTGGG	3 cut(s)	1366, 2052, 2471
BpmI	CTGGAG	2 cut(s)	243, 1830
Bpu1102I	GCTNAGC	1 cut(s)	1171
BpuEI	CTTGAG	2 cut(s)	1985, 2444
BsaAI	YACGTR	1 cut(s)	1802
BsaBI	GATNNNNATC	2 cut(s)	69, 2568
BsaI	GGTCTC	2 cut(s)	1986, 2305
BsaJI	CCNNGG	4 cut(s)	83, 486, 1194, 2131
BsaWI	WCCGGW	1 cut(s)	715
BsaXI	ACNNNNNCTCC	4 cut(s)	1828, 1858, 2604, 2634
Bsc4I	CCNNNNNNNGG	3 cut(s)	783, 1095, 2338
Bse118I	RCCGGY	1 cut(s)	1677
Bse3DI	GCAATG	1 cut(s)	1329
Bse8I	GATNNNNATC	2 cut(s)	69, 2568
BseAI	TCCGGA	1 cut(s)	715
BseBI	CCWGG	3 cut(s)	1259, 1673, 2672
BseDI	CCNNGG	4 cut(s)	83, 486, 1194, 2131
BseJI	GATNNNNATC	2 cut(s)	69, 2568
BseLI	CCNNNNNNNGG	3 cut(s)	783, 1095, 2338
BseMI	GCAATG	1 cut(s)	1329
BseMII	CTCAG	7 cut(s)	22, 69, 419, 1185, 1783, 2316, 2478
BseRI	GAGGAG	2 cut(s)	1541, 2639
BseXI	GCAGC	2 cut(s)	2276, 2341
BseYI	CCCAGC	4 cut(s)	1693, 1954, 2020, 2206
BsgI	GTGCAG	1 cut(s)	2603
BshFI	GGCC	4 cut(s)	1034, 1513, 1681, 2369
BsiHKAI	GWGCWC	1 cut(s)	1551
BsiHKCI	CYCGRG	1 cut(s)	1884
BsiSI	CCGG	3 cut(s)	716, 853, 1678
BslFI	GGGAC	2 cut(s)	656, 1610
BslI	CCNNNNNNNGG	3 cut(s)	783, 1095, 2338
BsmAI	GTCTC	5 cut(s)	523, 1492, 1724, 1986, 2305
BsmBI	CGTCTC	1 cut(s)	1724
BsmFI	GGGAC	2 cut(s)	656, 1610
BsmI	GAATGC	3 cut(s)	1370, 2186, 2228
BsnI	GGCC	4 cut(s)	1034, 1513, 1681, 2369
Bso31I	GGTCTC	2 cut(s)	1986, 2305
BsoBI	CYCGRG	1 cut(s)	1884
Bsp1286I	GDGCHC	1 cut(s)	1551
Bsp13I	TCCGGA	1 cut(s)	715
Bsp1407I	TGTACA	1 cut(s)	1948
Bsp1720I	GCTNAGC	1 cut(s)	1171
Bsp19I	CCATGG	1 cut(s)	83
BspACI	CCGC	4 cut(s)	25, 588, 1361, 2017
BspANI	GGCC	4 cut(s)	1034, 1513, 1681, 2369
BspCNI	CTCAG	7 cut(s)	21, 70, 418, 1184, 1782, 2315, 2479
BspEI	TCCGGA	1 cut(s)	715
BspHI	TCATGA	1 cut(s)	2572
BspLI	GGNNCC	4 cut(s)	89, 112, 880, 1051
BspMAI	CTGCAG	1 cut(s)	703
BspMI	ACCTGC	1 cut(s)	2398
BspOI	GCTAGC	1 cut(s)	921
BspPI	GGATC	7 cut(s)	72, 82, 95, 498, 1155, 2366, 2577
BspTNI	GGTCTC	2 cut(s)	1986, 2305
BsrDI	GCAATG	1 cut(s)	1329
BsrFI	RCCGGY	1 cut(s)	1677
BsrGI	TGTACA	1 cut(s)	1948
BssAI	RCCGGY	1 cut(s)	1677
BssECI	CCNNGG	4 cut(s)	83, 486, 1194, 2131
BssNAI	GTATAC	2 cut(s)	118, 250
BssT1I	CCWWGG	3 cut(s)	83, 486, 2131
Bst1107I	GTATAC	2 cut(s)	118, 250
Bst2UI	CCWGG	3 cut(s)	1259, 1673, 2672
Bst4CI	ACNGT	8 cut(s)	115, 375, 499, 757, 973, 1505, 1601, 2648
Bst6I	CTCTTC	1 cut(s)	2673
BstAPI	GCANNNNNTGC	2 cut(s)	1922, 2156
BstAUI	TGTACA	1 cut(s)	1948
BstBAI	YACGTR	1 cut(s)	1802
BstC8I	GCNNGC	3 cut(s)	919, 1818, 2528
BstDEI	CTNAG	7 cut(s)	8, 78, 405, 1171, 1769, 2302, 2487
BstDSI	CCRYGG	1 cut(s)	83
BstEII	GGTNACC	1 cut(s)	1654
BstENI	CCTNNNNNAGG	1 cut(s)	2336
BstHHI	GCGC	1 cut(s)	1329
BstMAI	GTCTC	5 cut(s)	523, 1492, 1724, 1986, 2305
BstMWI	GCNNNNNNNGC	5 cut(s)	10, 1822, 1922, 2156, 2273
BstNI	CCWGG	3 cut(s)	1259, 1673, 2672
BstNSI	RCATGY	3 cut(s)	238, 364, 2530
BstPI	GGTNACC	1 cut(s)	1654
BstSCI	CCNGG	3 cut(s)	1257, 1671, 2670
BstSFI	CTRYAG	4 cut(s)	699, 1008, 1290, 1986
BstV1I	GCAGC	2 cut(s)	2276, 2341
BstX2I	RGATCY	5 cut(s)	64, 87, 504, 1160, 2563
BstXI	CCANNNNNNTGG	1 cut(s)	2509
BstYI	RGATCY	5 cut(s)	64, 87, 504, 1160, 2563
BstZ17I	GTATAC	2 cut(s)	118, 250
BsuI	GTATCC	3 cut(s)	1748, 2247, 2394
BsuRI	GGCC	4 cut(s)	1034, 1513, 1681, 2369
BtgI	CCRYGG	1 cut(s)	83
BtsI	GCAGTG	1 cut(s)	215
BtsIMutI	CAGTG	6 cut(s)	165, 215, 582, 1510, 1904, 2040
BveI	ACCTGC	1 cut(s)	2398
Cac8I	GCNNGC	3 cut(s)	919, 1818, 2528
CaiI	CAGNNNCTG	1 cut(s)	1925
CciI	TCATGA	1 cut(s)	2572
CfoI	GCGC	1 cut(s)	1329
Cfr10I	RCCGGY	1 cut(s)	1677
Cfr13I	GGNCC	4 cut(s)	1191, 1675, 1680, 2086
CseI	GACGC	1 cut(s)	2444
Csp6I	GTAC	7 cut(s)	402, 835, 908, 1126, 1687, 1783, 1949
CviQI	GTAC	7 cut(s)	402, 835, 908, 1126, 1687, 1783, 1949
DdeI	CTNAG	7 cut(s)	8, 78, 405, 1171, 1769, 2302, 2487
DraIII	CACNNNGTG	1 cut(s)	433
EaeI	YGGCCR	2 cut(s)	1032, 2367
Eam1104I	CTCTTC	1 cut(s)	2673
EarI	CTCTTC	1 cut(s)	2673
EciI	GGCGGA	1 cut(s)	1376
Ecl136II	GAGCTC	1 cut(s)	1549
Eco130I	CCWWGG	3 cut(s)	83, 486, 2131
Eco24I	GRGCYC	1 cut(s)	1551
Eco31I	GGTCTC	2 cut(s)	1986, 2305
Eco32I	GATATC	2 cut(s)	19, 1700
Eco47I	GGWCC	3 cut(s)	1191, 1675, 2086
Eco53kI	GAGCTC	1 cut(s)	1549
Eco57I	CTGAAG	2 cut(s)	2082, 2280
Eco88I	CYCGRG	1 cut(s)	1884
Eco91I	GGTNACC	1 cut(s)	1654
EcoICRI	GAGCTC	1 cut(s)	1549
EcoNI	CCTNNNNNAGG	1 cut(s)	2336
EcoO65I	GGTNACC	1 cut(s)	1654
EcoRII	CCWGG	3 cut(s)	1257, 1671, 2670
EcoRV	GATATC	2 cut(s)	19, 1700
EcoT14I	CCWWGG	3 cut(s)	83, 486, 2131
EcoT22I	ATGCAT	1 cut(s)	2228
EcoT38I	GRGCYC	1 cut(s)	1551
ErhI	CCWWGG	3 cut(s)	83, 486, 2131
Esp3I	CGTCTC	1 cut(s)	1724
FalI	AAGNNNNNCTT	4 cut(s)	1012, 1044, 1814, 1846
FaqI	GGGAC	2 cut(s)	656, 1610
FauNDI	CATATG	2 cut(s)	337, 1343
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	3 cut(s)	117, 249, 1960
Fnu4HI	GCNGC	3 cut(s)	2018, 2265, 2355
FriOI	GRGCYC	1 cut(s)	1551
Fsp4HI	GCNGC	3 cut(s)	2018, 2265, 2355
FspBI	CTAG	3 cut(s)	918, 1526, 2507
GlaI	GCGC	1 cut(s)	1328
GluI	GCNGC	3 cut(s)	2018, 2265, 2355
GsaI	CCCAGC	4 cut(s)	1697, 1958, 2024, 2210
GsuI	CTGGAG	2 cut(s)	243, 1830
HaeIII	GGCC	4 cut(s)	1034, 1513, 1681, 2369
HapII	CCGG	3 cut(s)	716, 853, 1678
HgaI	GACGC	1 cut(s)	2444
HhaI	GCGC	1 cut(s)	1329
Hin6I	GCGC	1 cut(s)	1327
HinP1I	GCGC	1 cut(s)	1327
HindIII	AAGCTT	1 cut(s)	2048
HpaII	CCGG	3 cut(s)	716, 853, 1678
HphI	GGTGA	5 cut(s)	602, 1661, 1666, 2618, 2703
Hpy166II	GTNNAC	7 cut(s)	118, 250, 402, 848, 1609, 1961, 2040
Hpy188III	TCNNGA	8 cut(s)	108, 508, 716, 902, 1847, 1884, 2567, 2573
Hpy8I	GTNNAC	7 cut(s)	118, 250, 402, 848, 1609, 1961, 2040
HpyAV	CCTTC	6 cut(s)	410, 869, 1033, 1089, 1503, 2603
HpyCH4III	ACNGT	8 cut(s)	115, 375, 499, 757, 973, 1505, 1601, 2648
HpyCH4IV	ACGT	4 cut(s)	817, 1253, 1381, 1801
HpyF10VI	GCNNNNNNNGC	5 cut(s)	10, 1822, 1922, 2156, 2273
HpyF3I	CTNAG	7 cut(s)	8, 78, 405, 1171, 1769, 2302, 2487
HpySE526I	ACGT	4 cut(s)	817, 1253, 1381, 1801
HspAI	GCGC	1 cut(s)	1327
Kpn2I	TCCGGA	1 cut(s)	715
Ksp22I	TGATCA	1 cut(s)	622
LmnI	GCTCC	4 cut(s)	224, 878, 992, 1554
Lsp1109I	GCAGC	2 cut(s)	2276, 2341
LweI	GCATC	8 cut(s)	426, 535, 1528, 1891, 1912, 2116, 2159, 2620
MaeI	CTAG	3 cut(s)	918, 1526, 2507
MaeII	ACGT	4 cut(s)	817, 1253, 1381, 1801
MaeIII	GTNAC	5 cut(s)	135, 163, 442, 1055, 1654
MboII	GAAGA	8 cut(s)	115, 514, 2075, 2106, 2231, 2552, 2609, 2690
MflI	RGATCY	5 cut(s)	64, 87, 504, 1160, 2563
MhlI	GDGCHC	1 cut(s)	1551
MlyI	GAGTC	4 cut(s)	106, 2435, 2630, 2690
MmeI	TCCRAC	7 cut(s)	350, 374, 970, 1859, 1875, 2220, 2664
Mph1103I	ATGCAT	1 cut(s)	2228
MroI	TCCGGA	1 cut(s)	715
MseI	TTAA	9 cut(s)	54, 276, 450, 605, 891, 1398, 1632, 1743, 2270
MslI	CAYNNNNRTG	3 cut(s)	1302, 1860, 2577
MspA1I	CMGCKG	2 cut(s)	1954, 2233
MspI	CCGG	3 cut(s)	716, 853, 1678
MspR9I	CCNGG	3 cut(s)	1259, 1673, 2672
Mva1269I	GAATGC	3 cut(s)	1370, 2186, 2228
MvaI	CCWGG	3 cut(s)	1259, 1673, 2672
MwoI	GCNNNNNNNGC	5 cut(s)	10, 1822, 1922, 2156, 2273
NcoI	CCATGG	1 cut(s)	83
NdeI	CATATG	2 cut(s)	337, 1343
NheI	GCTAGC	1 cut(s)	917
NlaIV	GGNNCC	4 cut(s)	89, 112, 880, 1051
NmuCI	GTSAC	5 cut(s)	135, 163, 442, 1055, 1654
NsiI	ATGCAT	1 cut(s)	2228
NspI	RCATGY	3 cut(s)	238, 364, 2530
PaeI	GCATGC	1 cut(s)	2530
PaeR7I	CTCGAG	1 cut(s)	1884
PagI	TCATGA	1 cut(s)	2572
PciI	ACATGT	1 cut(s)	234
PctI	GAATGC	3 cut(s)	1370, 2186, 2228
PfeI	GAWTC	6 cut(s)	511, 964, 1448, 1880, 2003, 2360
PfoI	TCCNGGA	1 cut(s)	1257
PkrI	GCNGC	3 cut(s)	2019, 2266, 2356
PleI	GAGTC	4 cut(s)	105, 2434, 2630, 2689
PpsI	GAGTC	4 cut(s)	105, 2434, 2630, 2689
Ppu21I	YACGTR	1 cut(s)	1802
PscI	ACATGT	1 cut(s)	234
PsiI	TTATAA	2 cut(s)	258, 731
Psp124BI	GAGCTC	1 cut(s)	1551
Psp1406I	AACGTT	1 cut(s)	1253
Psp6I	CCWGG	3 cut(s)	1257, 1671, 2670
PspEI	GGTNACC	1 cut(s)	1654
PspFI	CCCAGC	4 cut(s)	1693, 1954, 2020, 2206
PspGI	CCWGG	3 cut(s)	1257, 1671, 2670
PspN4I	GGNNCC	4 cut(s)	89, 112, 880, 1051
PspPI	GGNCC	4 cut(s)	1191, 1675, 1680, 2086
PstI	CTGCAG	1 cut(s)	703
PstNI	CAGNNNCTG	1 cut(s)	1925
PsuI	RGATCY	5 cut(s)	64, 87, 504, 1160, 2563
PvuII	CAGCTG	2 cut(s)	1954, 2233
RsaI	GTAC	7 cut(s)	403, 836, 909, 1127, 1688, 1784, 1950
RsaNI	GTAC	7 cut(s)	402, 835, 908, 1126, 1687, 1783, 1949
RseI	CAYNNNNRTG	3 cut(s)	1302, 1860, 2577
SacI	GAGCTC	1 cut(s)	1551
SaqAI	TTAA	9 cut(s)	54, 276, 450, 605, 891, 1398, 1632, 1743, 2270
SatI	GCNGC	3 cut(s)	2018, 2265, 2355
Sau96I	GGNCC	4 cut(s)	1191, 1675, 1680, 2086
SchI	GAGTC	4 cut(s)	106, 2435, 2630, 2690
ScrFI	CCNGG	3 cut(s)	1259, 1673, 2672
SduI	GDGCHC	1 cut(s)	1551
SfaNI	GCATC	8 cut(s)	426, 535, 1528, 1891, 1912, 2116, 2159, 2620
SfcI	CTRYAG	4 cut(s)	699, 1008, 1290, 1986
Sfr274I	CTCGAG	1 cut(s)	1884
SinI	GGWCC	3 cut(s)	1191, 1675, 2086
SlaI	CTCGAG	1 cut(s)	1884
SmiMI	CAYNNNNRTG	3 cut(s)	1302, 1860, 2577
SmlI	CTYRAG	3 cut(s)	1884, 1964, 2423
SmoI	CTYRAG	3 cut(s)	1884, 1964, 2423
SphI	GCATGC	1 cut(s)	2530
SsiI	CCGC	4 cut(s)	25, 588, 1361, 2017
SspMI	CTAG	3 cut(s)	918, 1526, 2507
SstI	GAGCTC	1 cut(s)	1551
StyD4I	CCNGG	3 cut(s)	1257, 1671, 2670
StyI	CCWWGG	3 cut(s)	83, 486, 2131
TaaI	ACNGT	8 cut(s)	115, 375, 499, 757, 973, 1505, 1601, 2648
TaiI	ACGT	4 cut(s)	820, 1256, 1384, 1804
TaqII	GACCGA	1 cut(s)	171
TatI	WGTACW	5 cut(s)	401, 1125, 1686, 1782, 1948
TauI	GCSGC	1 cut(s)	2020
TfiI	GAWTC	6 cut(s)	511, 964, 1448, 1880, 2003, 2360
Tru1I	TTAA	9 cut(s)	54, 276, 450, 605, 891, 1398, 1632, 1743, 2270
Tru9I	TTAA	9 cut(s)	54, 276, 450, 605, 891, 1398, 1632, 1743, 2270
TscAI	CASTG	6 cut(s)	172, 215, 589, 1510, 1911, 2047
TseFI	GTSAC	5 cut(s)	135, 163, 442, 1055, 1654
TseI	GCWGC	2 cut(s)	2264, 2354
Tsp45I	GTSAC	5 cut(s)	135, 163, 442, 1055, 1654
TspRI	CASTG	6 cut(s)	172, 215, 589, 1510, 1911, 2047
VpaK11BI	GGWCC	3 cut(s)	1191, 1675, 2086
XagI	CCTNNNNNAGG	1 cut(s)	2336
XapI	RAATTY	4 cut(s)	29, 532, 1181, 1934
XceI	RCATGY	3 cut(s)	238, 364, 2530
XcmI	CCANNNNNNNNNTGG	3 cut(s)	81, 1690, 2506
XhoI	CTCGAG	1 cut(s)	1884
XmiI	GTMKAC	3 cut(s)	117, 249, 1960
XspI	CTAG	3 cut(s)	918, 1526, 2507
Zsp2I	ATGCAT	1 cut(s)	2228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.