Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Reverse (-)

52866618 .. 52877040

Gene Structure 10423 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G032090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGCCTCTCCGACTGGAAATCAAGAGGAAACTAGCTCAAAGATCAGAGAGAGTAAAATCTGTGGATCTACATCCAACAGAACCATGGATATTGGCGAGTCTGTATTCAGGAACAGTGTGTATTTGGAACTACCAGACTCAGACAATGGCCAAGTCATTTGAGGTCACTGAGTTGCCAGTTAGGTCAGCCAAATTTATATCACGCAAGCAATGGGTTGTTGCTGGAGCTGACGATATGTTTATTCGTGTATACAACTACAACACAATGGATAAGGTTAAAGTATTTGAGGCCCATACTGACTACATTAGATGTGTGGCTGTCCATCCTACGCTCCCATATGTGTTATCATCATCTGATGATATGCTCATCAAGCTATGGGATTGGGACAAGGGTTGGATTTGTACTCAAATATTTGAAGGACATTCGCATTATGTGATGCAAGTGACATTTAATCCTAAAGATACCAACACCTTTGCTAGTGCGTCTCTTGATCGCACCATAAAGATATGGAATCTAGGCTCCCCTGACCCAAATTTTACACTGGATGCCCACCAGAAAGGTGTAAATTGCGTTGATTACTTTACGGGTGGTGATAAACCTTATCTCATTACCGGTTCCGATGATCACACTGCTAAGGTGTGGGACTATCAAACAAAAGGTTGTGTCCAGACACTTGAAGGCCATACACACAATGTTTCTGCTGTATGTTTTCATCCTGAACTCCCTATAATAATAACAGGGTCAGAGGACGGAACAGTACGAATATGGCATTCAACGACTTACAGGCTTGAGAACACATTGAATTATGGGCTTGAAAGAGTTTGGGCCATTGGATACATGAAGGGCTCACGACGGGTTGTGATTGGTTATGATGAAGGAACCATTATGGTAAAACTTGGACGTGAAGTACCTGTTGCTAGTATGGACAATGGTGGAAAGATCATTTGGGCTAAGCACAATGAAATTCAAACAGTGAACATTAAGAGTGTAGGCGCTGATTATGAGGTTACTGATGGAGAGAGGTTGCCATTGGCCGTCAAGGAGTTGGGCACATGTGATCTTTACCCCCAAAGCTTAAAGCACAACCCCAATGGGAGGTTTGTTGTAGTTTGTGGAGATGGAGAATACATTATATATACTGCTTTGGCCTGGAGAAATCGGTCGTTTGGTTCTGCTTTGGAATTTGCTTGGTCATCTGAAGGAGAGTATGCTGTTAGAGAGAGTACTTCAAAAATTAAAATTTTCACTAAAAACTTCCAGGAGAAAAGGAGTATTCGACCTACATTTTCAGCAGAACATATATATGGGGGAACTTTATTGGCGATGTGCTCAAATGATTTTATTTGCTTTTATGATTGGGCTGAGTGCAGATTGATTCGGAGAATTGATGTGAATGTGAAAAATCTCTATTGGGCTGATAGTGGCGATTTAGTTGCTATTGCCATTGACGCATCATTCTACATCCTGAAGTACAATCGAGATGTAGTCTCTTCTTATTTGGATAGCGGAAGACCTGTAGATGAACAAGGTGTAGAAGATGCATTTGAGCTTCTCCATGAAATGAATGAACGGGTCAGGACTGGTTTATGGGTTGGGGATTGCTTCATTTACAACAACTCGTCTTGGAGGCTTAATTATTGTGTTGGTGGAGAGGTAACTACAATGTTCCATTTAGACCGGCCAATGTACTTGTTAGGCTATCTTGCCAATCAAAGTCGGGTGTATCTGATAGACAAAGAGTTCAATGTGATGGGGTACACACTACTTTTGAGTTTGATTGAGTATAAGACACTTGTTATGCGTGGAGACCTTGAAAGGGCCAATGAACTTTTACCGTCAATTCCTAAAGACCATCATAACAGTGTGGCTCGTTTCTTGGAATCACGTGGTATGATAGAGGATGCACTTGAAGTTGCTACAGATCCTGATTACAGATTTGAGCTAGCCATTCAGCTTGGTAGATTAGAAATTGCAGAGGAAATTGCCAGTGAGGTGCAGAGTGAATCTAAATGGAAACAGTTAGGTGAACTAGCCATGTCTACCGGAAAGTTGGAAATGGCTGAGCAATGTTTGAAGCATGCCATGGACTTGAGTGGTTTATTACTGCTTTATTCATCTCTTGGAGATTCTGAGGGAATCTCAAGACTTGCATCCCTTGCTAAAGAACAGGGGAAGAACAATGTTGCATTCCTTTGTTTATTCATGTTGGGTCGATTGGAAGAGTGCCTTGAGCTGTTAGTCGAAAGCAATCGGATACCTGAGGCTGCTTTGATGGCTCGATCATATCTTCCAAGCAAGGTCTCAGAAATAGTAGCTATTTGGAGAAAAGACCTTAGCAAGGTTAACCCAAAAGCTGCTGAATCTTTGGCTGATCCTGAAGAGTATCCTAATTTGTTTGAGGATTGGCAAGTTGCGCTCTCGGTTGAATCTAGAGCTGCAGAGACAAGGGGTGTTTATCCTCCTGCAGAGGAGTATGTGAAACATGTTGATAAAACACACATGACCCTTGTGGAGGCTTTCAGGAACTTGCAAGTGGATGAAGAGAACTTGCAAACGGATGAAGAGCAGCCCCGAGAAAATGGAGACTCAACGCATGAGGTTTCTGAACTGAATGGGGAACAGACTGTAGAGGAACATGCAGCTGACCAGCACGAGGAAGGGAGCCAGGAGGAGGCAGTTGTGGTGGATGCTGATTCTACGGATGGCACTGTACTCATTAATGGTAACGAAGCTGAAGAAGACTGGGGTACGAAAAATGAAGGAACCTCGACAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

925

Amino Acids

104.75

Weight (kDa)

4.96

Isoelectric Point (pI)

34.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	3 - 171	3.3e-17	WDR3 second beta-propeller domain
Beta-prop_WDR3_1st	PF25173	9 - 155	7.5e-17	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	12 - 155	2.1e-21	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	15 - 131	4.5e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	16 - 185	2.9e-20	Prp19 WD40 domain
WDR55	PF24796	16 - 199	6.1e-15	WDR55
Beta-prop_THOC3	PF25174	20 - 192	3.8e-24	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	20 - 172	4.6e-15	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	31 - 170	4.9e-06	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	32 - 217	3.6e-16	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	35 - 193	3e-17	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	36 - 174	2.7e-08	WDR36/Utp21 second beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	39 - 173	6.4e-06	MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st	PF24817	58 - 131	5.4e-11	WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	68 - 172	7e-08	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	69 - 260	5.9e-14	TEP-1 second beta-propeller
Beta-prop_EIPR1	PF23609	79 - 170	3e-06	EIPR1 beta-propeller
WD40	PF00400	91 - 127	3.8e-08	WD domain, G-beta repeat
WD40	PF00400	133 - 171	7.3e-07	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	135 - 296	3.5e-27	WDR3 first beta-propeller domain
EIF3I	PF24805	138 - 228	7.9e-06	EIF3I
WDR55	PF24796	138 - 296	7.2e-16	WDR55
Beta-prop_RIG_2nd	PF23775	139 - 246	8.1e-06	RIG second beta-propeller
Beta-prop_WDR3_2nd	PF25172	140 - 260	2.7e-15	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	154 - 289	1.4e-29	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	164 - 256	2.8e-06	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	164 - 240	2.4e-06	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	177 - 215	5e-07	WD domain, G-beta repeat
Beta-prop_SCAP	PF24017	183 - 259	3.8e-06	SCAP Beta-propeller
WD40_Prp19	PF24814	183 - 267	1.7e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	184 - 295	7.6e-16	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	197 - 265	3.8e-06	CAF1B/HIR1 beta-propeller domain
WD40_WDHD1_1st	PF24817	198 - 268	3.6e-11	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	198 - 284	5.1e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR75_1st	PF23869	198 - 266	2.9e-06	WD repeat-containing protein 75 first beta-propeller
Beta-prop_EML_2	PF23414	199 - 296	1.8e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	199 - 292	5.1e-09	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	201 - 258	5.5e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 261	2e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	221 - 256	4e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 298	2.4e-06	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	9.7e-89	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	3.6e-82	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	249, 2039
AciI	CCGC	1 cut(s)	1506
AclWI	GGATC	3 cut(s)	72, 1916, 2366
AcoI	YGGCCR	3 cut(s)	147, 1032, 1679
AcsI	RAATTY	5 cut(s)	191, 532, 963, 1181, 1239
AcuI	CTGAAG	4 cut(s)	1218, 1487, 2397, 2754
AcvI	CACGTG	1 cut(s)	1886
AfaI	GTAC	9 cut(s)	403, 759, 909, 1225, 1472, 1688, 1757, 2712, 2749
AfiI	CCNNNNNNNGG	6 cut(s)	1095, 1816, 2338, 2512, 2653, 2671
AflIII	ACRYGT	2 cut(s)	1052, 2482
AgeI	ACCGGT	1 cut(s)	611
AjiI	CACGTC	1 cut(s)	902
AjnI	CCWGG	3 cut(s)	1148, 1257, 2664
AjuI	GAANNNNNNNTTGG	2 cut(s)	928, 960
Alw21I	GWGCWC	1 cut(s)	1331
Alw26I	GTCTC	6 cut(s)	489, 1492, 1800, 2305, 2435, 2577
AlwI	GGATC	3 cut(s)	72, 1916, 2366
AlwNI	CAGNNNCTG	1 cut(s)	1925
Ama87I	CYCGRG	1 cut(s)	2571
AoxI	GGCC	8 cut(s)	147, 288, 679, 825, 1032, 1146, 1679, 1818
ApeKI	GCWGC	5 cut(s)	2264, 2354, 2435, 2566, 2639
ApoI	RAATTY	5 cut(s)	191, 532, 963, 1181, 1239
AseI	ATTAAT	1 cut(s)	2718
AsiGI	ACCGGT	1 cut(s)	611
AspLEI	GCGC	2 cut(s)	995, 2416
AspS9I	GGNCC	3 cut(s)	289, 825, 1818
AsuHPI	GGTGA	2 cut(s)	602, 2036
AsuNHI	GCTAGC	1 cut(s)	1942
AvaI	CYCGRG	1 cut(s)	2571
AxyI	CCTNAGG	1 cut(s)	2259
BaeGI	GKGCMC	1 cut(s)	1052
BalI	TGGCCA	1 cut(s)	149
BanII	GRGCYC	1 cut(s)	848
BauI	CACGAG	1 cut(s)	2651
BbrPI	CACGTG	1 cut(s)	1886
BbsI	GAAGAC	2 cut(s)	1516, 2745
Bbv12I	GWGCWC	1 cut(s)	1331
BbvI	GCAGC	5 cut(s)	2251, 2341, 2422, 2578, 2651
BccI	CCATC	7 cut(s)	330, 1007, 1112, 1744, 1860, 2266, 2696
BceAI	ACGGC	1 cut(s)	1019
BcgI	CGANNNNNNTGC	2 cut(s)	1415, 1449
BciT130I	CCWGG	3 cut(s)	1150, 1259, 2666
BciVI	GTATCC	3 cut(s)	827, 2247, 2394
BclI	TGATCA	1 cut(s)	622
BcoDI	GTCTC	6 cut(s)	489, 1492, 1800, 2305, 2435, 2577
BfaI	CTAG	7 cut(s)	32, 477, 515, 918, 1943, 2030, 2430
BfmI	CTRYAG	5 cut(s)	1515, 1917, 2436, 2463, 2625
BfoI	RGCGCY	1 cut(s)	996
BfuI	GTATCC	3 cut(s)	827, 2247, 2394
BisI	GCNGC	5 cut(s)	2265, 2355, 2436, 2567, 2640
BlpI	GCTNAGC	2 cut(s)	951, 2061
BlsI	GCNGC	5 cut(s)	2266, 2356, 2437, 2568, 2641
BmcAI	AGTACT	1 cut(s)	1225
Bme1390I	CCNGG	3 cut(s)	1150, 1259, 2666
BmeT110I	CYCGRG	1 cut(s)	2571
BmgBI	CACGTC	1 cut(s)	902
BmgT120I	GGNCC	3 cut(s)	289, 825, 1818
BmiI	GGNNCC	5 cut(s)	520, 616, 880, 2663, 2764
BmrFI	CCNGG	3 cut(s)	1150, 1259, 2666
BmrI	ACTGGG	1 cut(s)	2752
BmsI	GCATC	7 cut(s)	426, 535, 1460, 1528, 1891, 2159, 2677
BmtI	GCTAGC	1 cut(s)	1946
BmuI	ACTGGG	1 cut(s)	2752
BpiI	GAAGAC	2 cut(s)	1516, 2745
BplI	GAGNNNNNCTC	2 cut(s)	2123, 2155
BpmI	CTGGAG	2 cut(s)	243, 1171
Bpu10I	CCTNAGC	2 cut(s)	633, 2333
Bpu1102I	GCTNAGC	2 cut(s)	951, 2061
BpuEI	CTTGAG	4 cut(s)	809, 2110, 2125, 2249
BsaAI	YACGTR	1 cut(s)	1886
BsaBI	GATNNNNATC	1 cut(s)	69
BsaI	GGTCTC	2 cut(s)	1800, 2305
BsaJI	CCNNGG	2 cut(s)	83, 2082
BsaWI	WCCGGW	2 cut(s)	611, 2042
Bsc4I	CCNNNNNNNGG	6 cut(s)	1095, 1816, 2338, 2512, 2653, 2671
Bse118I	RCCGGY	2 cut(s)	611, 1677
Bse1I	ACTGG	6 cut(s)	18, 176, 546, 1585, 1986, 2747
Bse21I	CCTNAGG	1 cut(s)	2259
Bse3DI	GCAATG	2 cut(s)	215, 2072
Bse8I	GATNNNNATC	1 cut(s)	69
BseBI	CCWGG	3 cut(s)	1150, 1259, 2666
BseDI	CCNNGG	2 cut(s)	83, 2082
BseJI	GATNNNNATC	1 cut(s)	69
BseLI	CCNNNNNNNGG	6 cut(s)	1095, 1816, 2338, 2512, 2653, 2671
BseMI	GCAATG	2 cut(s)	215, 2072
BseMII	CTCAG	7 cut(s)	152, 159, 1353, 2052, 2121, 2250, 2316
BseNI	ACTGG	6 cut(s)	18, 176, 546, 1585, 1986, 2747
BseRI	GAGGAG	2 cut(s)	2483, 2684
BseSI	GKGCMC	1 cut(s)	1052
BseXI	GCAGC	5 cut(s)	2251, 2341, 2422, 2578, 2651
BsgI	GTGCAG	2 cut(s)	1387, 2015
Bsh1285I	CGRYCG	1 cut(s)	1163
BshFI	GGCC	8 cut(s)	149, 290, 681, 827, 1034, 1148, 1681, 1820
BshTI	ACCGGT	1 cut(s)	611
BsiEI	CGRYCG	1 cut(s)	1163
BsiHKAI	GWGCWC	1 cut(s)	1331
BsiHKCI	CYCGRG	1 cut(s)	2571
BsiSI	CCGG	3 cut(s)	612, 1678, 2043
BslFI	GGGAC	2 cut(s)	398, 656
BslI	CCNNNNNNNGG	6 cut(s)	1095, 1816, 2338, 2512, 2653, 2671
BsmAI	GTCTC	6 cut(s)	489, 1492, 1800, 2305, 2435, 2577
BsmBI	CGTCTC	1 cut(s)	489
BsmFI	GGGAC	2 cut(s)	398, 656
BsmI	GAATGC	2 cut(s)	769, 2186
BsnI	GGCC	8 cut(s)	149, 290, 681, 827, 1034, 1148, 1681, 1820
Bso31I	GGTCTC	2 cut(s)	1800, 2305
BsoBI	CYCGRG	1 cut(s)	2571
Bsp1286I	GDGCHC	3 cut(s)	848, 1052, 1331
Bsp143I	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
Bsp1720I	GCTNAGC	2 cut(s)	951, 2061
Bsp19I	CCATGG	2 cut(s)	83, 2082
BspACI	CCGC	1 cut(s)	1506
BspANI	GGCC	8 cut(s)	149, 290, 681, 827, 1034, 1148, 1681, 1820
BspCNI	CTCAG	7 cut(s)	151, 160, 1354, 2053, 2122, 2251, 2315
BspLI	GGNNCC	5 cut(s)	520, 616, 880, 2663, 2764
BspMAI	CTGCAG	2 cut(s)	2440, 2467
BspOI	GCTAGC	1 cut(s)	1946
BspPI	GGATC	3 cut(s)	72, 1916, 2366
BspQI	GCTCTTC	1 cut(s)	2556
BspTNI	GGTCTC	2 cut(s)	1800, 2305
BsrDI	GCAATG	2 cut(s)	215, 2072
BsrFI	RCCGGY	2 cut(s)	611, 1677
BsrI	ACTGG	6 cut(s)	18, 176, 546, 1585, 1986, 2747
BssAI	RCCGGY	2 cut(s)	611, 1677
BssECI	CCNNGG	2 cut(s)	83, 2082
BssMI	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
BssNAI	GTATAC	1 cut(s)	250
BssSI	CACGAG	1 cut(s)	2651
BssT1I	CCWWGG	2 cut(s)	83, 2082
Bst1107I	GTATAC	1 cut(s)	250
Bst2BI	CACGAG	1 cut(s)	2651
Bst2UI	CCWGG	3 cut(s)	1150, 1259, 2666
Bst4CI	ACNGT	8 cut(s)	115, 757, 973, 1836, 1862, 2019, 2626, 2710
Bst6I	CTCTTC	5 cut(s)	1495, 2214, 2373, 2535, 2556
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstBAI	YACGTR	1 cut(s)	1886
BstC8I	GCNNGC	3 cut(s)	206, 1944, 2079
BstDSI	CCRYGG	2 cut(s)	83, 2082
BstENI	CCTNNNNNAGG	2 cut(s)	2336, 2510
BstH2I	RGCGCY	1 cut(s)	996
BstHHI	GCGC	2 cut(s)	995, 2416
BstKTI	GATC	9 cut(s)	44, 67, 493, 625, 942, 1060, 1924, 2282, 2374
BstMAI	GTCTC	6 cut(s)	489, 1492, 1800, 2305, 2435, 2577
BstMBI	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
BstMCI	CGRYCG	1 cut(s)	1163
BstMWI	GCNNNNNNNGC	5 cut(s)	370, 1448, 2156, 2273, 2413
BstNI	CCWGG	3 cut(s)	1150, 1259, 2666
BstNSI	RCATGY	4 cut(s)	1056, 2081, 2486, 2639
BstSCI	CCNGG	3 cut(s)	1148, 1257, 2664
BstSFI	CTRYAG	5 cut(s)	1515, 1917, 2436, 2463, 2625
BstSLI	GKGCMC	1 cut(s)	1052
BstV1I	GCAGC	5 cut(s)	2251, 2341, 2422, 2578, 2651
BstV2I	GAAGAC	2 cut(s)	1516, 2745
BstX2I	RGATCY	2 cut(s)	64, 1921
BstYI	RGATCY	2 cut(s)	64, 1921
BstZ17I	GTATAC	1 cut(s)	250
Bsu36I	CCTNAGG	1 cut(s)	2259
BsuI	GTATCC	3 cut(s)	827, 2247, 2394
BsuRI	GGCC	8 cut(s)	149, 290, 681, 827, 1034, 1148, 1681, 1820
BtgI	CCRYGG	2 cut(s)	83, 2082
BtgZI	GCGATG	1 cut(s)	1337
BtrI	CACGTC	1 cut(s)	902
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	8 cut(s)	120, 165, 539, 627, 978, 1867, 1993, 2706
Cac8I	GCNNGC	3 cut(s)	206, 1944, 2079
CaiI	CAGNNNCTG	1 cut(s)	1925
CfoI	GCGC	2 cut(s)	995, 2416
Cfr10I	RCCGGY	2 cut(s)	611, 1677
Cfr13I	GGNCC	3 cut(s)	289, 825, 1818
CseI	GACGC	2 cut(s)	471, 1457
Csp6I	GTAC	9 cut(s)	402, 758, 908, 1224, 1471, 1687, 1756, 2711, 2748
CspAI	ACCGGT	1 cut(s)	611
CviQI	GTAC	9 cut(s)	402, 758, 908, 1224, 1471, 1687, 1756, 2711, 2748
DpnI	GATC	9 cut(s)	43, 66, 492, 624, 941, 1059, 1923, 2281, 2373
DpnII	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
EaeI	YGGCCR	3 cut(s)	147, 1032, 1679
Eam1104I	CTCTTC	5 cut(s)	1495, 2214, 2373, 2535, 2556
EarI	CTCTTC	5 cut(s)	1495, 2214, 2373, 2535, 2556
Eco130I	CCWWGG	2 cut(s)	83, 2082
Eco24I	GRGCYC	1 cut(s)	848
Eco31I	GGTCTC	2 cut(s)	1800, 2305
Eco57I	CTGAAG	4 cut(s)	1218, 1487, 2397, 2754
Eco72I	CACGTG	1 cut(s)	1886
Eco81I	CCTNAGG	1 cut(s)	2259
Eco88I	CYCGRG	1 cut(s)	2571
EcoNI	CCTNNNNNAGG	2 cut(s)	2336, 2510
EcoRII	CCWGG	3 cut(s)	1148, 1257, 2664
EcoT14I	CCWWGG	2 cut(s)	83, 2082
EcoT22I	ATGCAT	1 cut(s)	1543
EcoT38I	GRGCYC	1 cut(s)	848
ErhI	CCWWGG	2 cut(s)	83, 2082
Esp3I	CGTCTC	1 cut(s)	489
FalI	AAGNNNNNCTT	2 cut(s)	2211, 2243
FaqI	GGGAC	2 cut(s)	398, 656
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	2 cut(s)	249, 2039
Fnu4HI	GCNGC	5 cut(s)	2265, 2355, 2436, 2567, 2640
FriOI	GRGCYC	1 cut(s)	848
Fsp4HI	GCNGC	5 cut(s)	2265, 2355, 2436, 2567, 2640
FspBI	CTAG	7 cut(s)	32, 477, 515, 918, 1943, 2030, 2430
GlaI	GCGC	2 cut(s)	994, 2415
GluI	GCNGC	5 cut(s)	2265, 2355, 2436, 2567, 2640
GsuI	CTGGAG	2 cut(s)	243, 1171
HaeII	RGCGCY	1 cut(s)	996
HaeIII	GGCC	8 cut(s)	149, 290, 681, 827, 1034, 1148, 1681, 1820
HapII	CCGG	3 cut(s)	612, 1678, 2043
HgaI	GACGC	2 cut(s)	471, 1457
HhaI	GCGC	2 cut(s)	995, 2416
Hin6I	GCGC	2 cut(s)	993, 2414
HinP1I	GCGC	2 cut(s)	993, 2414
HincII	GTYRAC	1 cut(s)	2344
HindII	GTYRAC	1 cut(s)	2344
HindIII	AAGCTT	1 cut(s)	1072
HpaI	GTTAAC	1 cut(s)	2344
HpaII	CCGG	3 cut(s)	612, 1678, 2043
HphI	GGTGA	2 cut(s)	602, 2036
Hpy166II	GTNNAC	6 cut(s)	250, 976, 1758, 2027, 2040, 2344
Hpy8I	GTNNAC	6 cut(s)	250, 976, 1758, 2027, 2040, 2344
Hpy99I	CGWCG	1 cut(s)	855
HpyAV	CCTTC	7 cut(s)	410, 671, 835, 869, 1193, 2651, 2753
HpyCH4III	ACNGT	8 cut(s)	115, 757, 973, 1836, 1862, 2019, 2626, 2710
HpyCH4IV	ACGT	2 cut(s)	901, 1885
HpyF10VI	GCNNNNNNNGC	5 cut(s)	370, 1448, 2156, 2273, 2413
HpySE526I	ACGT	2 cut(s)	901, 1885
HspAI	GCGC	2 cut(s)	993, 2414
Ksp22I	TGATCA	1 cut(s)	622
KspAI	GTTAAC	1 cut(s)	2344
Kzo9I	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
LguI	GCTCTTC	1 cut(s)	2556
LmnI	GCTCC	4 cut(s)	224, 336, 524, 2661
Lsp1109I	GCAGC	5 cut(s)	2251, 2341, 2422, 2578, 2651
LweI	GCATC	7 cut(s)	426, 535, 1460, 1528, 1891, 2159, 2677
MaeI	CTAG	7 cut(s)	32, 477, 515, 918, 1943, 2030, 2430
MaeII	ACGT	2 cut(s)	901, 1885
MaeIII	GTNAC	5 cut(s)	163, 442, 1006, 1654, 2723
MalI	GATC	9 cut(s)	43, 66, 492, 624, 941, 1059, 1923, 2281, 2373
MboI	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
MflI	RGATCY	2 cut(s)	64, 1921
MhlI	GDGCHC	3 cut(s)	848, 1052, 1331
MlsI	TGGCCA	1 cut(s)	149
MluNI	TGGCCA	1 cut(s)	149
MlyI	GAGTC	3 cut(s)	106, 130, 2579
MmeI	TCCRAC	4 cut(s)	34, 98, 374, 2031
Mox20I	TGGCCA	1 cut(s)	149
Mph1103I	ATGCAT	1 cut(s)	1543
MscI	TGGCCA	1 cut(s)	149
MseI	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
MslI	CAYNNNNRTG	2 cut(s)	1302, 1860
Msp20I	TGGCCA	1 cut(s)	149
MspA1I	CMGCKG	1 cut(s)	2642
MspI	CCGG	3 cut(s)	612, 1678, 2043
MspR9I	CCNGG	3 cut(s)	1150, 1259, 2666
Mva1269I	GAATGC	2 cut(s)	769, 2186
MvaI	CCWGG	3 cut(s)	1150, 1259, 2666
MwoI	GCNNNNNNNGC	5 cut(s)	370, 1448, 2156, 2273, 2413
NcoI	CCATGG	2 cut(s)	83, 2082
NdeI	CATATG	1 cut(s)	337
NdeII	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
NheI	GCTAGC	1 cut(s)	1942
NlaIV	GGNNCC	5 cut(s)	520, 616, 880, 2663, 2764
NmuCI	GTSAC	2 cut(s)	163, 442
NsiI	ATGCAT	1 cut(s)	1543
NspI	RCATGY	4 cut(s)	1056, 2081, 2486, 2639
PaeI	GCATGC	1 cut(s)	2081
PciI	ACATGT	2 cut(s)	1052, 2482
PciSI	GCTCTTC	1 cut(s)	2556
PctI	GAATGC	2 cut(s)	769, 2186
PfeI	GAWTC	9 cut(s)	511, 1375, 1880, 2003, 2126, 2136, 2360, 2426, 2693
PfoI	TCCNGGA	1 cut(s)	1257
PinAI	ACCGGT	1 cut(s)	611
PkrI	GCNGC	5 cut(s)	2266, 2356, 2437, 2568, 2641
PleI	GAGTC	3 cut(s)	105, 130, 2579
PmaCI	CACGTG	1 cut(s)	1886
PmlI	CACGTG	1 cut(s)	1886
PpsI	GAGTC	3 cut(s)	105, 130, 2579
Ppu21I	YACGTR	1 cut(s)	1886
PscI	ACATGT	2 cut(s)	1052, 2482
PshBI	ATTAAT	1 cut(s)	2718
Psp6I	CCWGG	3 cut(s)	1148, 1257, 2664
PspCI	CACGTG	1 cut(s)	1886
PspGI	CCWGG	3 cut(s)	1148, 1257, 2664
PspN4I	GGNNCC	5 cut(s)	520, 616, 880, 2663, 2764
PspPI	GGNCC	3 cut(s)	289, 825, 1818
PstI	CTGCAG	2 cut(s)	2440, 2467
PstNI	CAGNNNCTG	1 cut(s)	1925
PsuI	RGATCY	2 cut(s)	64, 1921
PvuII	CAGCTG	1 cut(s)	2642
RsaI	GTAC	9 cut(s)	403, 759, 909, 1225, 1472, 1688, 1757, 2712, 2749
RsaNI	GTAC	9 cut(s)	402, 758, 908, 1224, 1471, 1687, 1756, 2711, 2748
RseI	CAYNNNNRTG	2 cut(s)	1302, 1860
SapI	GCTCTTC	1 cut(s)	2556
SaqAI	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
SatI	GCNGC	5 cut(s)	2265, 2355, 2436, 2567, 2640
Sau3AI	GATC	9 cut(s)	41, 64, 490, 622, 939, 1057, 1921, 2279, 2371
Sau96I	GGNCC	3 cut(s)	289, 825, 1818
ScaI	AGTACT	1 cut(s)	1225
SchI	GAGTC	3 cut(s)	106, 130, 2579
ScrFI	CCNGG	3 cut(s)	1150, 1259, 2666
SduI	GDGCHC	3 cut(s)	848, 1052, 1331
SfaNI	GCATC	7 cut(s)	426, 535, 1460, 1528, 1891, 2159, 2677
SfcI	CTRYAG	5 cut(s)	1515, 1917, 2436, 2463, 2625
SmiMI	CAYNNNNRTG	2 cut(s)	1302, 1860
SmlI	CTYRAG	4 cut(s)	788, 2089, 2140, 2228
SmoI	CTYRAG	4 cut(s)	788, 2089, 2140, 2228
SphI	GCATGC	1 cut(s)	2081
SsiI	CCGC	1 cut(s)	1506
SspI	AATATT	1 cut(s)	411
SspMI	CTAG	7 cut(s)	32, 477, 515, 918, 1943, 2030, 2430
StyD4I	CCNGG	3 cut(s)	1148, 1257, 2664
StyI	CCWWGG	2 cut(s)	83, 2082
TaaI	ACNGT	8 cut(s)	115, 757, 973, 1836, 1862, 2019, 2626, 2710
TaiI	ACGT	2 cut(s)	904, 1888
TaqI	TCGA	6 cut(s)	1276, 1477, 2212, 2241, 2278, 2768
TaqII	GACCGA	1 cut(s)	1149
TatI	WGTACW	5 cut(s)	401, 1223, 1470, 1686, 2710
TfiI	GAWTC	9 cut(s)	511, 1375, 1880, 2003, 2126, 2136, 2360, 2426, 2693
Tru1I	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
Tru9I	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
TscAI	CASTG	8 cut(s)	120, 172, 546, 634, 978, 1867, 1993, 2713
TseFI	GTSAC	2 cut(s)	163, 442
TseI	GCWGC	5 cut(s)	2264, 2354, 2435, 2566, 2639
Tsp45I	GTSAC	2 cut(s)	163, 442
TspGWI	ACGGA	3 cut(s)	765, 2570, 2714
TspRI	CASTG	8 cut(s)	120, 172, 546, 634, 978, 1867, 1993, 2713
VspI	ATTAAT	1 cut(s)	2718
XagI	CCTNNNNNAGG	2 cut(s)	2336, 2510
XapI	RAATTY	5 cut(s)	191, 532, 963, 1181, 1239
XbaI	TCTAGA	1 cut(s)	2429
XceI	RCATGY	4 cut(s)	1056, 2081, 2486, 2639
XcmI	CCANNNNNNNNNTGG	1 cut(s)	81
XmiI	GTMKAC	2 cut(s)	249, 2039
XspI	CTAG	7 cut(s)	32, 477, 515, 918, 1943, 2030, 2430
ZrmI	AGTACT	1 cut(s)	1225
Zsp2I	ATGCAT	1 cut(s)	1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.