Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Forward (+)

21050720 .. 21061059

Gene Structure 10340 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1214300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2829 bp

ATGCCTCTCAGACTGGAAATCAAGAGAAAACTCGCTCAAAGATCAGAGAGAGTAAAATCTGTGGATCTGCATCCAACAGAACCATGGATTTTGGCAAGTTTATATTCAGGAACTGTGTGTATTTGGAACTACCAGACGCAGACCTTGGCTAAGTCTTTTGAGGTCACAGAGTTACCAGTTAGGTCAGCCAAATTTATTGCTCGCAAGCAATGGGTTGTTGCCGGAGCTGATGACATGTTCATTCGTGTATACAATTACAATACAATGGATAAGGTTAAAGTATTTGAGGCACATACTGACTACATTAGATGTGTGGCTGTTCATCCTACACTCCCGTATGTGCTGTCATCATCCGATGATATGCTTATCAAGCTCTGGGATTGGGACAAGGGTTGGGTTTGTACTCAGATATTTGAAGGACACTCCCATTATGTGATGCAAGTGACATTTAATCCGAAAGATACCAACACATTTGCAAGTGCATCTCTTGATCGCACCATAAAGATATGGAATCTAGGCTCCCCAGACCCAAATTTTACATTGGATGCCCATCAGAAAGGAGTTAATTGTGTCGATTACTTTACGGGTGGTGATAGGCCTTATCTAATTACTGGATCTGATGATCACACTGCTAAGGTGTGGGACTATCAAACCAAAAGTTGTGTCCAGACACTTGATGGCCATACACACAATGTTTCTGCTGTATGTTTCCATCCTGAGCTTCCTATAATAATAACAGGGTCTGAGGATGGAACAGTTCGAATATGGCATTCAACCACTTACAGGCTTGAGAATACATTGAATTATGGGCTTGAAAGAGTTTGGGCTATTGGATACATGAAGAGCTCAAGGCGGGTTGTACTTGGTTATGACGAAGGAACAATTATGGTAAAACTGGGCCGTGAAGTACCTGTGGCAAGTATGGACAATAGTGGAAAGATCATTTGGGCTAAGCATAATGAAATTCAAACTGTGAACATTAAGAGTGTGGGAGCTGATTTCGAGGTTACTGATGGAGAGAGATTGCCTTTGGCTGTTAAGGAGTTGGGCACATGTGATCTTTACCCTCAGAGCTTAAGGCATAATCCCAATGGGAGGTTTGTTGTTGTTTGTGGAGATGGAGAGTACATTATATATACTGCTTTGGCCTGGAGAAATCGTTCATTTGGTTCTGCTTTGGAATTTGCTTGGTCAACTGATGGAGAATACGCTGTTAGAGAGAGTACTTCAAAGATTAAGATTTTCACTAAAAACTTCCAGGAGAAGAGGAGTATTCGACCTACATTTTCGGCTGAACATATTTACGGGGGAATTTTACTGGCAATGTGCTCAAATGACTTTATTTGCTTTTATGATTGGGCTGAATGTAGGTTGATTCGCCGAATTGATGTAACTGTTAAAAATCTCTATTGGGCTGATAGTGGTGATTTAGTGGCTGTTGCCAGTGATACATCATTCTACATCTTGAAATACAATCGGGACGTAGTCTCTTCTTACTTAGATAGCGGAACACCTGTAGATGAACAAGGTGTTGAAGATGCTTTTGAGCTTCTTCATGAAATGAATGAACGTGTCAGGACTGGTTTATGGGTTGGGGATTGTTTCATTTACAACAACTCCTCTTGGAGGCTTAATTACTGTGTTGGTGGCGAGGTAACCACAATGTTTCATTTAGACCGACCAATGTACTTGTTGGGTTATCTTGCCAATCAAAGTCGGGTGTTTCTAATAGACAAAGAGTTCAATGTAATGGGATACACACTACTTCTGAGTTTGATTGAATATAAGACTCTTGTAATGCGCGGAGACATTGAAAGGGCCAATGAAGTTTTGCCTTCAATTCCTAAAGAGCACCATAACAGTGTGGCTCGTTTCTTGGAATCACGAGGTATGATAGAGGAAGCACTTGAAGTGGCTACCGATCCTGATTACAGATTTGAACTAGCCATACAGCTTGGTAGATTAGAAATTGCGAAGGAAATTGCCACTGAGGTGCAAAGTGAGTCTAAATGGAAGCAGTTAGGTGAATTGGCTATGTCTGCTGGAAGCTTAGACATGGCCGAGGAATGTTTAAAGTATGCCATGGACTTGAGTGGTTTATTATTGCTCTATTCTTCTCTTGGAGATGTTGAAGGAATATCAAAACTTGCATCCCTTGCTAAAGAACAAGGGAAGAATAATGTTGCATACCTTTGTTTATTCATGTTGGGTCGATTGGAAGAGTGCCTTGATTTACTGGTGGAAAGCAATCGGATACCTGAGGCTGCTTTGATGGCTCGATCATATATTCCAGGCAAGGTTTCCGAGATAGTAGCTATTTGGAGAAAAGACCTGAGCAAGGTTAATCCGAAAGCTGCTGAGTCTTTGGCTGATCCTGAAGAATATCCCAATTTGTTTGATGATTGGCAAGTTGCCCTTTCTGTTGAATCGAAAGCTGCAGAGACAAGGGGTGTTTACCCTCCCGCAGAGGAGTACGTAAACCATGTTGATAAAGCACACATCACCCTTGTGGAGGCTTTCAGGAATATGCAATCGGATGAAGAGCTTCTTGAAAATGGGGAAGCAAATCATGGGGTTTCTGAACAGGACAGTGAAGAAAATGGGGAACGGGCTGCTGAAGAACATGATGGAGAAGAAGGTAGCCAGGAGGAGGCTGTTGTGGTGGATGCTGATTCTACGGATGCTGTACTCATCAACGGGAGCGAGGCTGACGAAGAGTGGGGTACGAATAATGAAGGACCCTCTGCTTACACCACACCACTAGGTCCTTATGAAGACTGCCGGTCCAGCTGTATGGATCCTTTACAGAGGCCTCTCGGAAGAGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

943

Amino Acids

106.28

Weight (kDa)

4.88

Isoelectric Point (pI)

36.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	3 - 171	1e-16	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	8 - 111	2.2e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	9 - 157	4.1e-17	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	12 - 172	5.2e-27	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	15 - 131	4.7e-06	WDR36/Utp21 second beta-propeller domain
WDR55	PF24796	16 - 196	1.3e-14	WDR55
WD40_Prp19	PF24814	16 - 185	8.6e-20	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	20 - 172	5.2e-15	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	31 - 170	4.5e-06	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	32 - 215	2e-15	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	35 - 226	1e-21	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	37 - 174	2.5e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	38 - 192	2.4e-22	THOC3 beta-propeller domain
WD40_MABP1-WDR62_1st	PF24780	41 - 173	1e-05	MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st	PF24817	58 - 131	4.9e-11	WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	69 - 170	2.2e-07	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	70 - 260	8e-14	TEP-1 second beta-propeller
Beta-prop_EIPR1	PF23609	79 - 170	1.5e-06	EIPR1 beta-propeller
WD40	PF00400	91 - 127	3.9e-08	WD domain, G-beta repeat
WD40	PF00400	133 - 171	4.9e-07	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	134 - 296	6.3e-28	WDR3 first beta-propeller domain
EIF3I	PF24805	138 - 228	5.4e-06	EIF3I
Beta-prop_RIG_2nd	PF23775	139 - 246	5.7e-06	RIG second beta-propeller
WDR55	PF24796	139 - 296	1.7e-15	WDR55
Beta-prop_WDR3_2nd	PF25172	140 - 260	2.2e-15	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	154 - 293	5.8e-30	WDR5 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	157 - 256	2e-06	MABP1/WDR62 second WD40 domain
Beta-prop_EML	PF23409	164 - 240	1.4e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	169 - 256	4.9e-06	WDR36/Utp21 first beta-propeller
WD40	PF00400	177 - 215	3.9e-07	WD domain, G-beta repeat
Beta-prop_SCAP	PF24017	183 - 259	2.8e-06	SCAP Beta-propeller
WD40_Prp19	PF24814	183 - 274	1.2e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	184 - 296	5e-16	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	197 - 265	2.4e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR75_1st	PF23869	198 - 267	3.9e-06	WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st	PF24817	198 - 320	1e-11	WDHD1 first WD40 domain
WD40_CDC20-Fz	PF24807	198 - 288	1.5e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR90_POC16_2nd	PF23393	199 - 257	3e-07	WDR90/POC16, second beta-propeller
Beta-prop_EML_2	PF23414	199 - 296	1.2e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	201 - 292	1.4e-09	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 261	1.4e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	220 - 256	3.4e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 298	1.6e-06	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	1.6e-87	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	1.2e-80	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	249
AccII	CGCG	1 cut(s)	1803
AciI	CCGC	4 cut(s)	853, 1506, 1803, 2463
AclWI	GGATC	6 cut(s)	72, 622, 1916, 2366, 2792, 2805
AcoI	YGGCCR	2 cut(s)	679, 2058
AcsI	RAATTY	5 cut(s)	191, 532, 963, 1181, 1311
AcuI	CTGAAG	2 cut(s)	2397, 2637
AfaI	GTAC	9 cut(s)	403, 861, 909, 1127, 1225, 1688, 2474, 2688, 2725
AfiI	CCNNNNNNNGG	6 cut(s)	783, 1095, 1626, 2338, 2512, 2650
AflII	CTTAAG	1 cut(s)	1075
AflIII	ACRYGT	3 cut(s)	234, 1052, 1570
AhdI	GACNNNNNGTC	1 cut(s)	2782
AjnI	CCWGG	4 cut(s)	1148, 1257, 2290, 2643
AjuI	GAANNNNNNNTTGG	2 cut(s)	928, 960
AleI	CACNNNNGTG	2 cut(s)	1991, 2507
AloI	GAACNNNNNNTCC	2 cut(s)	1144, 1176
Alw21I	GWGCWC	3 cut(s)	848, 1331, 1854
Alw26I	GTCTC	3 cut(s)	1492, 1800, 2435
AlwI	GGATC	6 cut(s)	72, 622, 1916, 2366, 2792, 2805
AlwNI	CAGNNNCTG	2 cut(s)	113, 743
AoxI	GGCC	7 cut(s)	596, 679, 898, 1146, 1818, 2058, 2810
ApeKI	GCWGC	4 cut(s)	2264, 2354, 2435, 2612
ApoI	RAATTY	5 cut(s)	191, 532, 963, 1181, 1311
ArsI	GACNNNNNNTTYG	2 cut(s)	648, 680
Asp700I	GAANNNNTTC	2 cut(s)	1159, 2544
AspLEI	GCGC	1 cut(s)	1803
AspS9I	GGNCC	5 cut(s)	898, 1818, 2738, 2765, 2784
AsuHPI	GGTGA	4 cut(s)	602, 1436, 2036, 2494
AsuII	TTCGAA	1 cut(s)	760
AvaII	GGWCC	3 cut(s)	2738, 2765, 2784
AxyI	CCTNAGG	1 cut(s)	2259
BaeGI	GKGCMC	1 cut(s)	1052
BalI	TGGCCA	1 cut(s)	681
BamHI	GGATCC	1 cut(s)	2797
BanII	GRGCYC	1 cut(s)	848
BauI	CACGAG	1 cut(s)	1884
BbsI	GAAGAC	1 cut(s)	2781
Bbv12I	GWGCWC	3 cut(s)	848, 1331, 1854
BbvI	GCAGC	4 cut(s)	2251, 2341, 2422, 2599
BccI	CCATC	9 cut(s)	558, 671, 720, 743, 1007, 1112, 1193, 2266, 2621
BceAI	ACGGC	1 cut(s)	885
BciT130I	CCWGG	4 cut(s)	1150, 1259, 2292, 2645
BciVI	GTATCC	3 cut(s)	827, 1748, 2247
BclI	TGATCA	1 cut(s)	622
BcoDI	GTCTC	3 cut(s)	1492, 1800, 2435
BfaI	CTAG	3 cut(s)	515, 1943, 2762
BfmI	CTRYAG	2 cut(s)	1515, 2436
BfrI	CTTAAG	1 cut(s)	1075
BfuI	GTATCC	3 cut(s)	827, 1748, 2247
BisI	GCNGC	4 cut(s)	2265, 2355, 2436, 2613
BlpI	GCTNAGC	1 cut(s)	951
BlsI	GCNGC	4 cut(s)	2266, 2356, 2437, 2614
BmcAI	AGTACT	1 cut(s)	1225
Bme1390I	CCNGG	4 cut(s)	1150, 1259, 2292, 2645
Bme18I	GGWCC	3 cut(s)	2738, 2765, 2784
BmeRI	GACNNNNNGTC	1 cut(s)	2782
BmgT120I	GGNCC	5 cut(s)	898, 1818, 2738, 2765, 2784
BmiI	GGNNCC	3 cut(s)	520, 2740, 2799
BmrFI	CCNGG	4 cut(s)	1150, 1259, 2292, 2645
BmrI	ACTGGG	1 cut(s)	905
BmsI	GCATC	8 cut(s)	79, 426, 491, 535, 1528, 2159, 2656, 2671
BmuI	ACTGGG	1 cut(s)	905
BpiI	GAAGAC	1 cut(s)	2781
BpmI	CTGGAG	1 cut(s)	1171
Bpu10I	CCTNAGC	3 cut(s)	633, 717, 2333
Bpu1102I	GCTNAGC	1 cut(s)	951
Bpu14I	TTCGAA	1 cut(s)	760
BpuEI	CTTGAG	3 cut(s)	809, 832, 2110
BsaAI	YACGTR	1 cut(s)	2476
BsaBI	GATNNNNATC	2 cut(s)	69, 549
BsaJI	CCNNGG	4 cut(s)	83, 144, 2061, 2082
BsaXI	ACNNNNNCTCC	4 cut(s)	1144, 1174, 1601, 1631
Bsc4I	CCNNNNNNNGG	6 cut(s)	783, 1095, 1626, 2338, 2512, 2650
Bse118I	RCCGGY	1 cut(s)	2781
Bse1I	ACTGG	8 cut(s)	18, 176, 616, 900, 1323, 1443, 1585, 2241
Bse21I	CCTNAGG	1 cut(s)	2259
Bse3DI	GCAATG	2 cut(s)	215, 1329
Bse8I	GATNNNNATC	2 cut(s)	69, 549
BseBI	CCWGG	4 cut(s)	1150, 1259, 2292, 2645
BseDI	CCNNGG	4 cut(s)	83, 144, 2061, 2082
BseJI	GATNNNNATC	2 cut(s)	69, 549
BseLI	CCNNNNNNNGG	6 cut(s)	783, 1095, 1626, 2338, 2512, 2650
BseMI	GCAATG	2 cut(s)	215, 1329
BseNI	ACTGG	8 cut(s)	18, 176, 616, 900, 1323, 1443, 1585, 2241
BseRI	GAGGAG	4 cut(s)	1282, 1609, 2483, 2663
BseSI	GKGCMC	1 cut(s)	1052
BseXI	GCAGC	4 cut(s)	2251, 2341, 2422, 2599
Bsh1236I	CGCG	1 cut(s)	1803
BshFI	GGCC	7 cut(s)	598, 681, 900, 1148, 1820, 2060, 2812
BsiHKAI	GWGCWC	3 cut(s)	848, 1331, 1854
BsiSI	CCGG	2 cut(s)	222, 2782
BslFI	GGGAC	3 cut(s)	398, 656, 1493
BslI	CCNNNNNNNGG	6 cut(s)	783, 1095, 1626, 2338, 2512, 2650
BsmAI	GTCTC	3 cut(s)	1492, 1800, 2435
BsmFI	GGGAC	3 cut(s)	398, 656, 1493
BsmI	GAATGC	1 cut(s)	769
BsnI	GGCC	7 cut(s)	598, 681, 900, 1148, 1820, 2060, 2812
Bsp119I	TTCGAA	1 cut(s)	760
Bsp1286I	GDGCHC	4 cut(s)	848, 1052, 1331, 1854
Bsp1720I	GCTNAGC	1 cut(s)	951
Bsp19I	CCATGG	2 cut(s)	83, 2082
BspACI	CCGC	4 cut(s)	853, 1506, 1803, 2463
BspANI	GGCC	7 cut(s)	598, 681, 900, 1148, 1820, 2060, 2812
BspFNI	CGCG	1 cut(s)	1803
BspHI	TCATGA	1 cut(s)	1555
BspLI	GGNNCC	3 cut(s)	520, 2740, 2799
BspMAI	CTGCAG	1 cut(s)	2440
BspPI	GGATC	6 cut(s)	72, 622, 1916, 2366, 2792, 2805
BspQI	GCTCTTC	2 cut(s)	836, 2535
BspT104I	TTCGAA	1 cut(s)	760
BspTI	CTTAAG	1 cut(s)	1075
BsrDI	GCAATG	2 cut(s)	215, 1329
BsrFI	RCCGGY	1 cut(s)	2781
BsrI	ACTGG	8 cut(s)	18, 176, 616, 900, 1323, 1443, 1585, 2241
BssAI	RCCGGY	1 cut(s)	2781
BssECI	CCNNGG	4 cut(s)	83, 144, 2061, 2082
BssNAI	GTATAC	1 cut(s)	250
BssSI	CACGAG	1 cut(s)	1884
BssT1I	CCWWGG	3 cut(s)	83, 144, 2082
Bst1107I	GTATAC	1 cut(s)	250
Bst2BI	CACGAG	1 cut(s)	1884
Bst2UI	CCWGG	4 cut(s)	1150, 1259, 2292, 2645
Bst4CI	ACNGT	7 cut(s)	115, 757, 973, 1396, 1640, 1862, 2591
Bst6I	CTCTTC	7 cut(s)	836, 1259, 1495, 2214, 2535, 2709, 2815
BstAFI	CTTAAG	1 cut(s)	1075
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstBAI	YACGTR	1 cut(s)	2476
BstBI	TTCGAA	1 cut(s)	760
BstC8I	GCNNGC	2 cut(s)	202, 206
BstDSI	CCRYGG	2 cut(s)	83, 2082
BstEII	GGTNACC	1 cut(s)	1654
BstENI	CCTNNNNNAGG	3 cut(s)	1624, 2336, 2510
BstFNI	CGCG	1 cut(s)	1803
BstHHI	GCGC	1 cut(s)	1803
BstMAI	GTCTC	3 cut(s)	1492, 1800, 2435
BstMWI	GCNNNNNNNGC	5 cut(s)	370, 2039, 2156, 2273, 2787
BstNI	CCWGG	4 cut(s)	1150, 1259, 2292, 2645
BstNSI	RCATGY	2 cut(s)	238, 1056
BstPI	GGTNACC	1 cut(s)	1654
BstSCI	CCNGG	4 cut(s)	1148, 1257, 2290, 2643
BstSFI	CTRYAG	2 cut(s)	1515, 2436
BstSLI	GKGCMC	1 cut(s)	1052
BstSNI	TACGTA	1 cut(s)	2476
BstUI	CGCG	1 cut(s)	1803
BstV1I	GCAGC	4 cut(s)	2251, 2341, 2422, 2599
BstV2I	GAAGAC	1 cut(s)	2781
BstX2I	RGATCY	3 cut(s)	64, 614, 2797
BstXI	CCANNNNNNTGG	1 cut(s)	2794
BstYI	RGATCY	3 cut(s)	64, 614, 2797
BstZ17I	GTATAC	1 cut(s)	250
Bsu36I	CCTNAGG	1 cut(s)	2259
BsuI	GTATCC	3 cut(s)	827, 1748, 2247
BsuRI	GGCC	7 cut(s)	598, 681, 900, 1148, 1820, 2060, 2812
BtgI	CCRYGG	2 cut(s)	83, 2082
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	5 cut(s)	627, 1450, 1867, 1986, 2596
Cac8I	GCNNGC	2 cut(s)	202, 206
CaiI	CAGNNNCTG	2 cut(s)	113, 743
CciI	TCATGA	1 cut(s)	1555
CfoI	GCGC	1 cut(s)	1803
Cfr10I	RCCGGY	1 cut(s)	2781
Cfr13I	GGNCC	5 cut(s)	898, 1818, 2738, 2765, 2784
CseI	GACGC	1 cut(s)	145
Csp6I	GTAC	9 cut(s)	402, 860, 908, 1126, 1224, 1687, 2473, 2687, 2724
CviQI	GTAC	9 cut(s)	402, 860, 908, 1126, 1224, 1687, 2473, 2687, 2724
DraI	TTTAAA	1 cut(s)	2073
DriI	GACNNNNNGTC	1 cut(s)	2782
EaeI	YGGCCR	2 cut(s)	679, 2058
Eam1104I	CTCTTC	7 cut(s)	836, 1259, 1495, 2214, 2535, 2709, 2815
Eam1105I	GACNNNNNGTC	1 cut(s)	2782
EarI	CTCTTC	7 cut(s)	836, 1259, 1495, 2214, 2535, 2709, 2815
Ecl136II	GAGCTC	1 cut(s)	846
Eco105I	TACGTA	1 cut(s)	2476
Eco130I	CCWWGG	3 cut(s)	83, 144, 2082
Eco147I	AGGCCT	2 cut(s)	598, 2812
Eco24I	GRGCYC	1 cut(s)	848
Eco47I	GGWCC	3 cut(s)	2738, 2765, 2784
Eco53kI	GAGCTC	1 cut(s)	846
Eco57I	CTGAAG	2 cut(s)	2397, 2637
Eco81I	CCTNAGG	1 cut(s)	2259
Eco91I	GGTNACC	1 cut(s)	1654
EcoICRI	GAGCTC	1 cut(s)	846
EcoNI	CCTNNNNNAGG	3 cut(s)	1624, 2336, 2510
EcoO109I	RGGNCCY	2 cut(s)	2738, 2765
EcoO65I	GGTNACC	1 cut(s)	1654
EcoRII	CCWGG	4 cut(s)	1148, 1257, 2290, 2643
EcoT14I	CCWWGG	3 cut(s)	83, 144, 2082
EcoT38I	GRGCYC	1 cut(s)	848
ErhI	CCWWGG	3 cut(s)	83, 144, 2082
FalI	AAGNNNNNCTT	6 cut(s)	2211, 2243, 2400, 2432, 2532, 2564
FaqI	GGGAC	3 cut(s)	398, 656, 1493
FauI	CCCGC	2 cut(s)	846, 2470
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	1 cut(s)	249
Fnu4HI	GCNGC	4 cut(s)	2265, 2355, 2436, 2613
FriOI	GRGCYC	1 cut(s)	848
Fsp4HI	GCNGC	4 cut(s)	2265, 2355, 2436, 2613
FspBI	CTAG	3 cut(s)	515, 1943, 2762
GlaI	GCGC	1 cut(s)	1802
GluI	GCNGC	4 cut(s)	2265, 2355, 2436, 2613
GsuI	CTGGAG	1 cut(s)	1171
HaeIII	GGCC	7 cut(s)	598, 681, 900, 1148, 1820, 2060, 2812
HapII	CCGG	2 cut(s)	222, 2782
HgaI	GACGC	1 cut(s)	145
HhaI	GCGC	1 cut(s)	1803
Hin6I	GCGC	1 cut(s)	1801
HinP1I	GCGC	1 cut(s)	1801
HincII	GTYRAC	1 cut(s)	1194
HindII	GTYRAC	1 cut(s)	1194
HindIII	AAGCTT	1 cut(s)	2047
HinfI	GANTC	8 cut(s)	511, 1375, 1789, 1880, 2003, 2360, 2426, 2672
HpaII	CCGG	2 cut(s)	222, 2782
HphI	GGTGA	4 cut(s)	602, 1436, 2036, 2494
Hpy166II	GTNNAC	5 cut(s)	250, 976, 1194, 2455, 2479
Hpy8I	GTNNAC	5 cut(s)	250, 976, 1194, 2455, 2479
HpyAV	CCTTC	7 cut(s)	410, 869, 1845, 1969, 2126, 2630, 2729
HpyCH4III	ACNGT	7 cut(s)	115, 757, 973, 1396, 1640, 1862, 2591
HpyCH4IV	ACGT	3 cut(s)	1482, 1570, 2475
HpyCH4V	TGCA	9 cut(s)	70, 439, 476, 482, 1996, 2150, 2186, 2438, 2530
HpyF10VI	GCNNNNNNNGC	5 cut(s)	370, 2039, 2156, 2273, 2787
HpySE526I	ACGT	3 cut(s)	1482, 1570, 2475
HspAI	GCGC	1 cut(s)	1801
Ksp22I	TGATCA	1 cut(s)	622
LguI	GCTCTTC	2 cut(s)	836, 2535
LmnI	GCTCC	4 cut(s)	224, 524, 992, 2700
Lsp1109I	GCAGC	4 cut(s)	2251, 2341, 2422, 2599
LweI	GCATC	8 cut(s)	79, 426, 491, 535, 1528, 2159, 2656, 2671
MaeI	CTAG	3 cut(s)	515, 1943, 2762
MaeII	ACGT	3 cut(s)	1482, 1570, 2475
MaeIII	GTNAC	6 cut(s)	163, 171, 442, 1006, 1390, 1654
MflI	RGATCY	3 cut(s)	64, 614, 2797
MhlI	GDGCHC	4 cut(s)	848, 1052, 1331, 1854
MlsI	TGGCCA	1 cut(s)	681
MluNI	TGGCCA	1 cut(s)	681
MlyI	GAGTC	3 cut(s)	1783, 2012, 2369
MmeI	TCCRAC	1 cut(s)	98
Mox20I	TGGCCA	1 cut(s)	681
MroXI	GAANNNNTTC	2 cut(s)	1159, 2544
MscI	TGGCCA	1 cut(s)	681
MslI	CAYNNNNRTG	3 cut(s)	1860, 1991, 2507
Msp20I	TGGCCA	1 cut(s)	681
MspA1I	CMGCKG	1 cut(s)	2790
MspCI	CTTAAG	1 cut(s)	1075
MspI	CCGG	2 cut(s)	222, 2782
MspR9I	CCNGG	4 cut(s)	1150, 1259, 2292, 2645
Mva1269I	GAATGC	1 cut(s)	769
MvaI	CCWGG	4 cut(s)	1150, 1259, 2292, 2645
MvnI	CGCG	1 cut(s)	1803
MwoI	GCNNNNNNNGC	5 cut(s)	370, 2039, 2156, 2273, 2787
NcoI	CCATGG	2 cut(s)	83, 2082
NlaIV	GGNNCC	3 cut(s)	520, 2740, 2799
NmeAIII	GCCGAG	1 cut(s)	2086
NmuCI	GTSAC	2 cut(s)	163, 442
NspI	RCATGY	2 cut(s)	238, 1056
NspV	TTCGAA	1 cut(s)	760
OliI	CACNNNNGTG	2 cut(s)	1991, 2507
PagI	TCATGA	1 cut(s)	1555
PceI	AGGCCT	2 cut(s)	598, 2812
PciI	ACATGT	2 cut(s)	234, 1052
PciSI	GCTCTTC	2 cut(s)	836, 2535
PctI	GAATGC	1 cut(s)	769
PdmI	GAANNNNTTC	2 cut(s)	1159, 2544
PfeI	GAWTC	5 cut(s)	511, 1375, 1880, 2426, 2672
PflFI	GACNNNGTC	1 cut(s)	1484
PfoI	TCCNGGA	1 cut(s)	1257
PkrI	GCNGC	4 cut(s)	2266, 2356, 2437, 2614
PleI	GAGTC	3 cut(s)	1783, 2011, 2368
PpsI	GAGTC	3 cut(s)	1783, 2011, 2368
Ppu21I	YACGTR	1 cut(s)	2476
PpuMI	RGGWCCY	2 cut(s)	2738, 2765
PscI	ACATGT	2 cut(s)	234, 1052
Psp124BI	GAGCTC	1 cut(s)	848
Psp5II	RGGWCCY	2 cut(s)	2738, 2765
Psp6I	CCWGG	4 cut(s)	1148, 1257, 2290, 2643
PspEI	GGTNACC	1 cut(s)	1654
PspGI	CCWGG	4 cut(s)	1148, 1257, 2290, 2643
PspN4I	GGNNCC	3 cut(s)	520, 2740, 2799
PspPI	GGNCC	5 cut(s)	898, 1818, 2738, 2765, 2784
PspPPI	RGGWCCY	2 cut(s)	2738, 2765
PsrI	GAACNNNNNNTAC	2 cut(s)	1932, 1964
PstI	CTGCAG	1 cut(s)	2440
PstNI	CAGNNNCTG	2 cut(s)	113, 743
PsuI	RGATCY	3 cut(s)	64, 614, 2797
PsyI	GACNNNGTC	1 cut(s)	1484
PvuII	CAGCTG	1 cut(s)	2790
RsaI	GTAC	9 cut(s)	403, 861, 909, 1127, 1225, 1688, 2474, 2688, 2725
RsaNI	GTAC	9 cut(s)	402, 860, 908, 1126, 1224, 1687, 2473, 2687, 2724
RseI	CAYNNNNRTG	3 cut(s)	1860, 1991, 2507
SacI	GAGCTC	1 cut(s)	848
SapI	GCTCTTC	2 cut(s)	836, 2535
SatI	GCNGC	4 cut(s)	2265, 2355, 2436, 2613
Sau96I	GGNCC	5 cut(s)	898, 1818, 2738, 2765, 2784
ScaI	AGTACT	1 cut(s)	1225
SchI	GAGTC	3 cut(s)	1783, 2012, 2369
ScrFI	CCNGG	4 cut(s)	1150, 1259, 2292, 2645
SduI	GDGCHC	4 cut(s)	848, 1052, 1331, 1854
SfaNI	GCATC	8 cut(s)	79, 426, 491, 535, 1528, 2159, 2656, 2671
SfcI	CTRYAG	2 cut(s)	1515, 2436
SfuI	TTCGAA	1 cut(s)	760
SinI	GGWCC	3 cut(s)	2738, 2765, 2784
SmiMI	CAYNNNNRTG	3 cut(s)	1860, 1991, 2507
SmlI	CTYRAG	4 cut(s)	788, 847, 1075, 2089
SmoI	CTYRAG	4 cut(s)	788, 847, 1075, 2089
SnaBI	TACGTA	1 cut(s)	2476
SseBI	AGGCCT	2 cut(s)	598, 2812
SsiI	CCGC	4 cut(s)	853, 1506, 1803, 2463
SspMI	CTAG	3 cut(s)	515, 1943, 2762
SstI	GAGCTC	1 cut(s)	848
StuI	AGGCCT	2 cut(s)	598, 2812
StyD4I	CCNGG	4 cut(s)	1148, 1257, 2290, 2643
StyI	CCWWGG	3 cut(s)	83, 144, 2082
TaaI	ACNGT	7 cut(s)	115, 757, 973, 1396, 1640, 1862, 2591
TaiI	ACGT	3 cut(s)	1485, 1573, 2478
TaqI	TCGA	7 cut(s)	573, 760, 1002, 1276, 2212, 2278, 2429
TaqII	GACCGA	1 cut(s)	1692
TatI	WGTACW	6 cut(s)	401, 859, 1125, 1223, 1686, 2686
TfiI	GAWTC	5 cut(s)	511, 1375, 1880, 2426, 2672
TscAI	CASTG	5 cut(s)	634, 1450, 1867, 1993, 2596
TseFI	GTSAC	2 cut(s)	163, 442
TseI	GCWGC	4 cut(s)	2264, 2354, 2435, 2612
Tsp45I	GTSAC	2 cut(s)	163, 442
TspGWI	ACGGA	1 cut(s)	2693
TspRI	CASTG	5 cut(s)	634, 1450, 1867, 1993, 2596
Tth111I	GACNNNGTC	1 cut(s)	1484
Vha464I	CTTAAG	1 cut(s)	1075
VpaK11BI	GGWCC	3 cut(s)	2738, 2765, 2784
XagI	CCTNNNNNAGG	3 cut(s)	1624, 2336, 2510
XapI	RAATTY	5 cut(s)	191, 532, 963, 1181, 1311
XceI	RCATGY	2 cut(s)	238, 1056
XcmI	CCANNNNNNNNNTGG	2 cut(s)	81, 674
XmiI	GTMKAC	1 cut(s)	249
XmnI	GAANNNNTTC	2 cut(s)	1159, 2544
XspI	CTAG	3 cut(s)	515, 1943, 2762
ZrmI	AGTACT	1 cut(s)	1225

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.