Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Reverse (-)

24190236 .. 24201201

Gene Structure 10966 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g31010.t7

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2778 bp

ATGCCTCTCCGACTGGAAATCAAGAGGAAACTTGCTCAAAGATCAGAGAGAGTAAAATCTGTGGATCTACATCCAACAGAACCATGGATATTGGCGAGTCTATATTCTGGAACAGTGTGTATTTGGAACTACCAAACTCAGACCATGGCTAAGTCATTTGAGGTCACTGAGTTACCAGTTAGGTCAGCCAAATTTATATCACGCAAGCAATGGGTTGTTGCTGGAGCTGACGATATGTTCATTCGTGTATACAACTACAACACCATGGATAAGATTAAAGTATTTGAGGCCCATACTGACTACATTAGATGTGTGGCTGTTCATCCTACACTCCCATATGTGTTATCATCATCTGATGATATGCTCATCAAGCTATGGGATTGGGACAAGGGTTGGATCTGTACTCAGATATTTGAAGGACATTCACATTATGTGATGCAAGTGACATTTAATCCAAAAGACACCAACACCTTTGCAAGTGCGTCTCTTGATCGCACCATAAAGATATGGAATCTAGGCTCTCCTGATCCAAATTTTACACTGGATGCCCACCAGAAAGGTGTAAATTGCGTTGATTACTTTACGGGTGGTGACAAACCTTATCTAATTACTGGTTCCGATGATCACACTGCTAAGGTGTGGGACTACCAAACAAAAGGTTGTGTCCAGACACTTGAAGGCCATACACACAATGTTTCTGCTGTATGTTTTCATCCCGAACTCCCTATAATAATAACTGGATCAGAGGATGGAACAGTACGAATATGGCATTCAACCACTTACAGGCTTGAAAACACGTTGAATTACGGGCTTGAAAGAGTTTGGGCTATTGGATACATGAAGGGCTCACGACGGGTTGTGATTGGTTATGATGAAGGAACCATTATGGTAAAACTTGGACGTGAAGTGCCTGTTGCTAGTATGGACAATAGTGGAAAGATCATTTGGGCTAAGCACAATGAAATTCAGACAGTGAACATTAAGAGTGTGGGTGCTGATTATGAGGTTACTGATGGAGAGAGATTGCCTTTGGCCGTCAAGGAGTTGGGCACATGCGATCTTTACCCCCAAAGCTTAAAGCACAACCCCAATGGGAGGTTTGTTGTAGTTTGTGGAGATGGAGAGTACATTATATATACTGCTTTGGCATGGAGAAATCGGTCATTTGGTTCTGCTTTGGAATTTGCTTGGTCATCTGATGGAGAGTATGCTGTTCGAGAGAGTACTTCAAAAATTAAAATTTTCACTAAAAACTTCCAGGAGAAAAGGAGTATTCGACCGACATTTTCAGCTGAACATATATATGGTGGAACCTTATTGGCAATGTGCTCAAATGATTTTATTTGCTTTTATGATTGGGCTGAGTGCAGATTGATTCGTAGAATTGATGTGAATGTGAAGAATCTCTATTGGGCTGATAGTGGCGATTTAGTTGCTATTGCCAGTGACGCCTCATTCTACATCCTGAAGTACAACCGGGATGTGGTCTCCTCTTATTTGGATAGCGGAAGACCTGTAGATGAACAAGGTGTAGAAGATGCATTTGAGCTTCTCCATGAAATGAATGAACGAGTCAGGACTGGTATATGGGTTGGCGATTGCTTCATTTACAACAACTCATCCTGGAGGCTTAATTATTGTGTTGGTGGGGAGGTAACTACAATGTTCCATTTAGACCGGCCAATGTACCTGTTAGGTTATCTTGCCAATCAAAGTCGGGTGTTTCTGATAGACAAAGAGTTCAATGTGATGGGGTACACACTACTTTTGAGTCTGATCGAATACAAGACACTTATGATGCGTGGAGACCTGGAAAGGGCCAATGCAGTTTTACCTTCAATTCCTAAAGACCATCATAACAGTGTGGCTCGTTTCTTGGAATCACGAGGTATGATAGAGGATGCACTTGAAGTTGCCACAGATCCTGATTACAGATTTGAGCTAGCCATTCAGCTTGGTAGATTGGAAATTGCAAAGGAAATTGCCAGTGAGGTGCAGAGTGAATCTAAATGGAAACAGCTAGGTGAACTAGCCATGTCTACTGGAAATTTGGAAATGGCTGAGCAATGTTTGAAGCATGCCATGGACTTGAGTGGTTTATTATTGCTTTATTCATCTCTTGGAGATGCTGAGGGAATCTCAACACTTGCATCCCTTGCTAAAGAACAAGGGAAGAACAATGTTGCATTCCTTTGTTTGTTCATACTGGGTCGCTTGGAAGAGTGCCTTGACCTGCTAGTTGAAAGCAATCGGATACCTGAGGCTGCTTTGATGGCTCGATCGTATCTTCCAAGCAAGGTTTCAGAAATAGTAGCTATTTGGAGAAAAGACCTTAGCAAGGTTAATTCAAAAGCTGCCGAATCTTTGGCTGATCCTGAACAGTATTCTAATTTGTTTGAAGATTGGCAAGTTGCTCTGTCGGTTGAATCTAGAGCCGCGGAGACAAGGGGTGTTTATCCTCCTGCAGAGGAGTATGTGAAACATGTTGATAAAACACACATGACCCTTGTGGAGGCTTTCAAGAACTTGCAAGTGGATGAAGAAAACTTGCAAATGGATGAAGAGGAGCCCCGTGAAAATGGAGACTCAAACCATGAGGTTTCTGAATTGAATGGGGAACAGACTGTAGAGAATCATGCAGATGAACATCATGAGGAAGGGAGCCAGGAGGAGGCTGTTGTCGTGGATGCTGATTCTACGGATGGCGCTGTACTCGTTAATGGTAGCGAAGCTGAAGAAGACTGGGGTACGAAAAATGAAGGAACCTCGACAGCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0005829 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

926

Amino Acids

104.56

Weight (kDa)

4.98

Isoelectric Point (pI)

32.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_2nd	PF25172	3 - 171	4.8e-17	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	8 - 111	1.5e-09	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	9 - 155	8.3e-17	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	12 - 154	2.4e-21	WDR5 beta-propeller domain
WDR55	PF24796	16 - 199	6.9e-15	WDR55
WD40_Prp19	PF24814	16 - 185	4.4e-20	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	17 - 174	2.3e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	20 - 172	7e-15	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	31 - 170	4.5e-06	WDR36/Utp21 first beta-propeller
WD40_Gbeta	PF25391	32 - 211	1.8e-14	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	35 - 193	2.8e-17	CDC20/Fizzy WD40 domain
WD40_MABP1-WDR62_1st	PF24780	39 - 173	9.1e-06	MABP1/WDR62 first WD40 domain
WD40_WDHD1_1st	PF24817	58 - 131	5.5e-11	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	60 - 192	8.2e-21	THOC3 beta-propeller domain
WD40_MABP1-WDR62_2nd	PF24782	68 - 172	6.3e-08	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	69 - 260	6.5e-14	TEP-1 second beta-propeller
Beta-prop_EIPR1	PF23609	81 - 170	3.5e-06	EIPR1 beta-propeller
WD40	PF00400	91 - 127	4.1e-08	WD domain, G-beta repeat
WD40	PF00400	133 - 171	7.3e-07	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	135 - 296	3.5e-27	WDR3 first beta-propeller domain
EIF3I	PF24805	138 - 229	7.7e-06	EIF3I
WDR55	PF24796	138 - 296	7.2e-16	WDR55
Beta-prop_RIG_2nd	PF23775	139 - 246	8.2e-06	RIG second beta-propeller
Beta-prop_WDR3_2nd	PF25172	140 - 260	2.6e-15	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	154 - 289	1.2e-29	WDR5 beta-propeller domain
Beta-prop_EML	PF23409	164 - 242	9.9e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	167 - 256	6.8e-06	WDR36/Utp21 first beta-propeller
WD40	PF00400	177 - 215	5e-07	WD domain, G-beta repeat
Beta-prop_SCAP	PF24017	183 - 259	4.1e-06	SCAP Beta-propeller
WD40_Prp19	PF24814	183 - 268	1.7e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	184 - 296	6.5e-16	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	197 - 265	3.6e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR75_1st	PF23869	198 - 266	2.9e-06	WD repeat-containing protein 75 first beta-propeller
WD40_CDC20-Fz	PF24807	198 - 284	5.1e-08	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	198 - 277	2.9e-11	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	199 - 296	1.7e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_Gbeta	PF25391	200 - 292	5.9e-09	G protein beta WD-40 repeat protein
Beta-prop_WDR90_POC16_2nd	PF23393	201 - 258	5.7e-07	WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 261	1.9e-06	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	221 - 256	4e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 298	2.1e-06	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	4.7e-89	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	2.4e-81	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2241
AccI	GTMKAC	2 cut(s)	249, 2039
AccII	CGCG	1 cut(s)	2438
AciI	CCGC	3 cut(s)	1506, 2436, 2438
AclWI	GGATC	6 cut(s)	72, 404, 521, 748, 1916, 2366
AcoI	YGGCCR	2 cut(s)	1032, 1679
AcsI	RAATTY	6 cut(s)	191, 532, 963, 1181, 1239, 2047
AcuI	CTGAAG	2 cut(s)	1487, 2754
AcyI	GRCGYC	1 cut(s)	1449
AfaI	GTAC	9 cut(s)	403, 759, 1127, 1225, 1472, 1688, 1757, 2712, 2749
AfiI	CCNNNNNNNGG	7 cut(s)	783, 1095, 1483, 1816, 2338, 2512, 2671
AflIII	ACRYGT	2 cut(s)	795, 2482
AjiI	CACGTC	1 cut(s)	902
AjnI	CCWGG	4 cut(s)	1257, 1622, 1809, 2664
AjuI	GAANNNNNNNTTGG	4 cut(s)	928, 960, 2284, 2316
Alw21I	GWGCWC	1 cut(s)	1331
Alw26I	GTCTC	5 cut(s)	489, 1492, 1800, 2435, 2577
AlwI	GGATC	6 cut(s)	72, 404, 521, 748, 1916, 2366
AlwNI	CAGNNNCTG	1 cut(s)	1925
AoxI	GGCC	5 cut(s)	288, 679, 1032, 1679, 1818
ApeKI	GCWGC	2 cut(s)	2264, 2354
ApoI	RAATTY	6 cut(s)	191, 532, 963, 1181, 1239, 2047
AspLEI	GCGC	1 cut(s)	2708
AspS9I	GGNCC	2 cut(s)	289, 1818
AsuC2I	CCSGG	1 cut(s)	1478
AsuHPI	GGTGA	2 cut(s)	602, 2036
AsuNHI	GCTAGC	1 cut(s)	1942
AxyI	CCTNAGG	1 cut(s)	2259
BaeGI	GKGCMC	1 cut(s)	1052
BanII	GRGCYC	2 cut(s)	848, 2571
BauI	CACGAG	1 cut(s)	1884
BbsI	GAAGAC	2 cut(s)	1516, 2745
Bbv12I	GWGCWC	1 cut(s)	1331
BbvCI	CCTCAGC	1 cut(s)	2130
BbvI	GCAGC	2 cut(s)	2251, 2341
BccI	CCATC	8 cut(s)	743, 1007, 1112, 1193, 1744, 1860, 2266, 2696
BceAI	ACGGC	1 cut(s)	1019
BcgI	CGANNNNNNTGC	2 cut(s)	1415, 1449
BciT130I	CCWGG	4 cut(s)	1259, 1624, 1811, 2666
BciVI	GTATCC	2 cut(s)	827, 2247
BclI	TGATCA	1 cut(s)	622
BcnI	CCSGG	1 cut(s)	1478
BcoDI	GTCTC	5 cut(s)	489, 1492, 1800, 2435, 2577
BfaI	CTAG	7 cut(s)	515, 918, 1943, 2021, 2030, 2237, 2430
BfmI	CTRYAG	3 cut(s)	1515, 2463, 2625
BfoI	RGCGCY	1 cut(s)	2709
BfuAI	ACCTGC	1 cut(s)	2241
BfuI	GTATCC	2 cut(s)	827, 2247
BisI	GCNGC	3 cut(s)	2265, 2355, 2436
BlpI	GCTNAGC	2 cut(s)	951, 2061
BlsI	GCNGC	3 cut(s)	2266, 2356, 2437
BmcAI	AGTACT	1 cut(s)	1225
Bme1390I	CCNGG	5 cut(s)	1259, 1478, 1624, 1811, 2666
BmgBI	CACGTC	1 cut(s)	902
BmgT120I	GGNCC	2 cut(s)	289, 1818
BmiI	GGNNCC	6 cut(s)	616, 880, 1312, 2568, 2663, 2764
BmrFI	CCNGG	5 cut(s)	1259, 1478, 1624, 1811, 2666
BmrI	ACTGGG	2 cut(s)	2216, 2752
BmsI	GCATC	8 cut(s)	426, 535, 1528, 1788, 1891, 2116, 2159, 2677
BmtI	GCTAGC	1 cut(s)	1946
BmuI	ACTGGG	2 cut(s)	2216, 2752
BpiI	GAAGAC	2 cut(s)	1516, 2745
BplI	GAGNNNNNCTC	2 cut(s)	2123, 2155
BpmI	CTGGAG	2 cut(s)	243, 1645
Bpu10I	CCTNAGC	3 cut(s)	633, 2130, 2333
Bpu1102I	GCTNAGC	2 cut(s)	951, 2061
BpuEI	CTTGAG	1 cut(s)	2110
BpuMI	CCSGG	1 cut(s)	1478
BsaBI	GATNNNNATC	2 cut(s)	69, 2646
BsaHI	GRCGYC	1 cut(s)	1449
BsaI	GGTCTC	2 cut(s)	1492, 1800
BsaJI	CCNNGG	5 cut(s)	83, 144, 264, 2082, 2436
Bsc4I	CCNNNNNNNGG	7 cut(s)	783, 1095, 1483, 1816, 2338, 2512, 2671
Bse118I	RCCGGY	1 cut(s)	1677
Bse21I	CCTNAGG	1 cut(s)	2259
Bse3DI	GCAATG	3 cut(s)	215, 1329, 2072
Bse8I	GATNNNNATC	2 cut(s)	69, 2646
BseBI	CCWGG	4 cut(s)	1259, 1624, 1811, 2666
BseDI	CCNNGG	5 cut(s)	83, 144, 264, 2082, 2436
BseJI	GATNNNNATC	2 cut(s)	69, 2646
BseLI	CCNNNNNNNGG	7 cut(s)	783, 1095, 1483, 1816, 2338, 2512, 2671
BseMI	GCAATG	3 cut(s)	215, 1329, 2072
BseMII	CTCAG	7 cut(s)	152, 159, 419, 1353, 2052, 2121, 2250
BseRI	GAGGAG	4 cut(s)	1480, 2483, 2579, 2684
BseSI	GKGCMC	1 cut(s)	1052
BseXI	GCAGC	2 cut(s)	2251, 2341
BsgI	GTGCAG	2 cut(s)	1387, 2015
Bsh1236I	CGCG	1 cut(s)	2438
Bsh1285I	CGRYCG	2 cut(s)	1280, 2282
BshFI	GGCC	5 cut(s)	290, 681, 1034, 1681, 1820
BsiEI	CGRYCG	2 cut(s)	1280, 2282
BsiHKAI	GWGCWC	1 cut(s)	1331
BsiSI	CCGG	2 cut(s)	1477, 1678
BslFI	GGGAC	2 cut(s)	398, 656
BslI	CCNNNNNNNGG	7 cut(s)	783, 1095, 1483, 1816, 2338, 2512, 2671
BsmAI	GTCTC	5 cut(s)	489, 1492, 1800, 2435, 2577
BsmBI	CGTCTC	1 cut(s)	489
BsmFI	GGGAC	2 cut(s)	398, 656
BsmI	GAATGC	2 cut(s)	769, 2186
BsnI	GGCC	5 cut(s)	290, 681, 1034, 1681, 1820
Bso31I	GGTCTC	2 cut(s)	1492, 1800
Bsp1286I	GDGCHC	4 cut(s)	848, 1052, 1331, 2571
Bsp1720I	GCTNAGC	2 cut(s)	951, 2061
Bsp19I	CCATGG	4 cut(s)	83, 144, 264, 2082
BspACI	CCGC	3 cut(s)	1506, 2436, 2438
BspANI	GGCC	5 cut(s)	290, 681, 1034, 1681, 1820
BspCNI	CTCAG	7 cut(s)	151, 160, 418, 1354, 2053, 2122, 2251
BspFNI	CGCG	1 cut(s)	2438
BspHI	TCATGA	1 cut(s)	2650
BspLI	GGNNCC	6 cut(s)	616, 880, 1312, 2568, 2663, 2764
BspMAI	CTGCAG	1 cut(s)	2467
BspMI	ACCTGC	1 cut(s)	2241
BspOI	GCTAGC	1 cut(s)	1946
BspPI	GGATC	6 cut(s)	72, 404, 521, 748, 1916, 2366
BspTNI	GGTCTC	2 cut(s)	1492, 1800
BsrDI	GCAATG	3 cut(s)	215, 1329, 2072
BsrFI	RCCGGY	1 cut(s)	1677
BssAI	RCCGGY	1 cut(s)	1677
BssECI	CCNNGG	5 cut(s)	83, 144, 264, 2082, 2436
BssNAI	GTATAC	1 cut(s)	250
BssNI	GRCGYC	1 cut(s)	1449
BssSI	CACGAG	1 cut(s)	1884
BssT1I	CCWWGG	4 cut(s)	83, 144, 264, 2082
Bst1107I	GTATAC	1 cut(s)	250
Bst2BI	CACGAG	1 cut(s)	1884
Bst2UI	CCWGG	4 cut(s)	1259, 1624, 1811, 2666
Bst4CI	ACNGT	6 cut(s)	115, 757, 973, 1862, 2382, 2626
Bst6I	CTCTTC	2 cut(s)	2214, 2556
BstACI	GRCGYC	1 cut(s)	1449
BstAPI	GCANNNNNTGC	1 cut(s)	2156
BstC8I	GCNNGC	3 cut(s)	206, 1944, 2079
BstDSI	CCRYGG	5 cut(s)	83, 144, 264, 2082, 2436
BstENI	CCTNNNNNAGG	2 cut(s)	2336, 2510
BstFNI	CGCG	1 cut(s)	2438
BstH2I	RGCGCY	1 cut(s)	2709
BstHHI	GCGC	1 cut(s)	2708
BstMAI	GTCTC	5 cut(s)	489, 1492, 1800, 2435, 2577
BstMCI	CGRYCG	2 cut(s)	1280, 2282
BstMWI	GCNNNNNNNGC	4 cut(s)	370, 1448, 2156, 2273
BstNI	CCWGG	4 cut(s)	1259, 1624, 1811, 2666
BstNSI	RCATGY	3 cut(s)	1056, 2081, 2486
BstSCI	CCNGG	5 cut(s)	1257, 1476, 1622, 1809, 2664
BstSFI	CTRYAG	3 cut(s)	1515, 2463, 2625
BstSLI	GKGCMC	1 cut(s)	1052
BstUI	CGCG	1 cut(s)	2438
BstV1I	GCAGC	2 cut(s)	2251, 2341
BstV2I	GAAGAC	2 cut(s)	1516, 2745
BstX2I	RGATCY	3 cut(s)	64, 396, 1921
BstYI	RGATCY	3 cut(s)	64, 396, 1921
BstZ17I	GTATAC	1 cut(s)	250
Bsu36I	CCTNAGG	1 cut(s)	2259
BsuI	GTATCC	2 cut(s)	827, 2247
BsuRI	GGCC	5 cut(s)	290, 681, 1034, 1681, 1820
BtgI	CCRYGG	5 cut(s)	83, 144, 264, 2082, 2436
BtrI	CACGTC	1 cut(s)	902
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	8 cut(s)	120, 165, 539, 627, 978, 1450, 1867, 1993
BveI	ACCTGC	1 cut(s)	2241
Cac8I	GCNNGC	3 cut(s)	206, 1944, 2079
CaiI	CAGNNNCTG	1 cut(s)	1925
CciI	TCATGA	1 cut(s)	2650
CfoI	GCGC	1 cut(s)	2708
Cfr10I	RCCGGY	1 cut(s)	1677
Cfr13I	GGNCC	2 cut(s)	289, 1818
Cfr42I	CCGCGG	1 cut(s)	2439
CseI	GACGC	2 cut(s)	471, 1457
Csp6I	GTAC	9 cut(s)	402, 758, 1126, 1224, 1471, 1687, 1756, 2711, 2748
CviQI	GTAC	9 cut(s)	402, 758, 1126, 1224, 1471, 1687, 1756, 2711, 2748
EaeI	YGGCCR	2 cut(s)	1032, 1679
Eam1104I	CTCTTC	2 cut(s)	2214, 2556
EarI	CTCTTC	2 cut(s)	2214, 2556
Eco130I	CCWWGG	4 cut(s)	83, 144, 264, 2082
Eco24I	GRGCYC	2 cut(s)	848, 2571
Eco31I	GGTCTC	2 cut(s)	1492, 1800
Eco57I	CTGAAG	2 cut(s)	1487, 2754
Eco81I	CCTNAGG	1 cut(s)	2259
EcoNI	CCTNNNNNAGG	2 cut(s)	2336, 2510
EcoRII	CCWGG	4 cut(s)	1257, 1622, 1809, 2664
EcoT14I	CCWWGG	4 cut(s)	83, 144, 264, 2082
EcoT22I	ATGCAT	1 cut(s)	1543
EcoT38I	GRGCYC	2 cut(s)	848, 2571
ErhI	CCWWGG	4 cut(s)	83, 144, 264, 2082
Esp3I	CGTCTC	1 cut(s)	489
FalI	AAGNNNNNCTT	2 cut(s)	2211, 2243
FaqI	GGGAC	2 cut(s)	398, 656
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	2 cut(s)	249, 2039
Fnu4HI	GCNGC	3 cut(s)	2265, 2355, 2436
FriOI	GRGCYC	2 cut(s)	848, 2571
Fsp4HI	GCNGC	3 cut(s)	2265, 2355, 2436
FspBI	CTAG	7 cut(s)	515, 918, 1943, 2021, 2030, 2237, 2430
GlaI	GCGC	1 cut(s)	2707
GluI	GCNGC	3 cut(s)	2265, 2355, 2436
GsuI	CTGGAG	2 cut(s)	243, 1645
HaeII	RGCGCY	1 cut(s)	2709
HaeIII	GGCC	5 cut(s)	290, 681, 1034, 1681, 1820
HapII	CCGG	2 cut(s)	1477, 1678
HgaI	GACGC	2 cut(s)	471, 1457
HhaI	GCGC	1 cut(s)	2708
Hin1I	GRCGYC	1 cut(s)	1449
Hin6I	GCGC	1 cut(s)	2706
HinP1I	GCGC	1 cut(s)	2706
HindIII	AAGCTT	1 cut(s)	1072
HpaII	CCGG	2 cut(s)	1477, 1678
HphI	GGTGA	2 cut(s)	602, 2036
Hpy166II	GTNNAC	5 cut(s)	250, 976, 1758, 2027, 2040
Hpy8I	GTNNAC	5 cut(s)	250, 976, 1758, 2027, 2040
Hpy99I	CGWCG	1 cut(s)	855
HpyAV	CCTTC	7 cut(s)	410, 671, 835, 869, 1845, 2651, 2753
HpyCH4III	ACNGT	6 cut(s)	115, 757, 973, 1862, 2382, 2626
HpyCH4IV	ACGT	2 cut(s)	797, 901
HpyF10VI	GCNNNNNNNGC	4 cut(s)	370, 1448, 2156, 2273
HpySE526I	ACGT	2 cut(s)	797, 901
Hsp92I	GRCGYC	1 cut(s)	1449
HspAI	GCGC	1 cut(s)	2706
Ksp22I	TGATCA	1 cut(s)	622
KspI	CCGCGG	1 cut(s)	2439
LmnI	GCTCC	3 cut(s)	224, 2566, 2661
Lsp1109I	GCAGC	2 cut(s)	2251, 2341
LweI	GCATC	8 cut(s)	426, 535, 1528, 1788, 1891, 2116, 2159, 2677
MaeI	CTAG	7 cut(s)	515, 918, 1943, 2021, 2030, 2237, 2430
MaeII	ACGT	2 cut(s)	797, 901
MaeIII	GTNAC	7 cut(s)	163, 171, 442, 590, 1006, 1445, 1654
MflI	RGATCY	3 cut(s)	64, 396, 1921
MhlI	GDGCHC	4 cut(s)	848, 1052, 1331, 2571
MlyI	GAGTC	4 cut(s)	106, 1581, 1780, 2579
MmeI	TCCRAC	3 cut(s)	34, 98, 374
Mph1103I	ATGCAT	1 cut(s)	1543
MseI	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
MslI	CAYNNNNRTG	3 cut(s)	1302, 1860, 2640
MspA1I	CMGCKG	2 cut(s)	1292, 2438
MspI	CCGG	2 cut(s)	1477, 1678
MspR9I	CCNGG	5 cut(s)	1259, 1478, 1624, 1811, 2666
Mva1269I	GAATGC	2 cut(s)	769, 2186
MvaI	CCWGG	4 cut(s)	1259, 1624, 1811, 2666
MvnI	CGCG	1 cut(s)	2438
MwoI	GCNNNNNNNGC	4 cut(s)	370, 1448, 2156, 2273
NciI	CCSGG	1 cut(s)	1478
NcoI	CCATGG	4 cut(s)	83, 144, 264, 2082
NdeI	CATATG	1 cut(s)	337
NheI	GCTAGC	1 cut(s)	1942
NlaIV	GGNNCC	6 cut(s)	616, 880, 1312, 2568, 2663, 2764
NmuCI	GTSAC	4 cut(s)	163, 442, 590, 1445
NsiI	ATGCAT	1 cut(s)	1543
NspI	RCATGY	3 cut(s)	1056, 2081, 2486
PaeI	GCATGC	1 cut(s)	2081
PagI	TCATGA	1 cut(s)	2650
PciI	ACATGT	1 cut(s)	2482
PctI	GAATGC	2 cut(s)	769, 2186
PfoI	TCCNGGA	2 cut(s)	1257, 1622
PkrI	GCNGC	3 cut(s)	2266, 2356, 2437
Ple19I	CGATCG	1 cut(s)	2282
PleI	GAGTC	4 cut(s)	105, 1580, 1779, 2579
PpsI	GAGTC	4 cut(s)	105, 1580, 1779, 2579
PscI	ACATGT	1 cut(s)	2482
Psp6I	CCWGG	4 cut(s)	1257, 1622, 1809, 2664
PspGI	CCWGG	4 cut(s)	1257, 1622, 1809, 2664
PspN4I	GGNNCC	6 cut(s)	616, 880, 1312, 2568, 2663, 2764
PspPI	GGNCC	2 cut(s)	289, 1818
PstI	CTGCAG	1 cut(s)	2467
PstNI	CAGNNNCTG	1 cut(s)	1925
PsuI	RGATCY	3 cut(s)	64, 396, 1921
PvuI	CGATCG	1 cut(s)	2282
PvuII	CAGCTG	1 cut(s)	1292
RsaI	GTAC	9 cut(s)	403, 759, 1127, 1225, 1472, 1688, 1757, 2712, 2749
RsaNI	GTAC	9 cut(s)	402, 758, 1126, 1224, 1471, 1687, 1756, 2711, 2748
RseI	CAYNNNNRTG	3 cut(s)	1302, 1860, 2640
SacII	CCGCGG	1 cut(s)	2439
SaqAI	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
SatI	GCNGC	3 cut(s)	2265, 2355, 2436
Sau96I	GGNCC	2 cut(s)	289, 1818
ScaI	AGTACT	1 cut(s)	1225
SchI	GAGTC	4 cut(s)	106, 1581, 1780, 2579
ScrFI	CCNGG	5 cut(s)	1259, 1478, 1624, 1811, 2666
SduI	GDGCHC	4 cut(s)	848, 1052, 1331, 2571
SfaNI	GCATC	8 cut(s)	426, 535, 1528, 1788, 1891, 2116, 2159, 2677
SfcI	CTRYAG	3 cut(s)	1515, 2463, 2625
Sfr303I	CCGCGG	1 cut(s)	2439
SgrBI	CCGCGG	1 cut(s)	2439
SmiMI	CAYNNNNRTG	3 cut(s)	1302, 1860, 2640
SmlI	CTYRAG	1 cut(s)	2089
SmoI	CTYRAG	1 cut(s)	2089
SphI	GCATGC	1 cut(s)	2081
SsiI	CCGC	3 cut(s)	1506, 2436, 2438
SspMI	CTAG	7 cut(s)	515, 918, 1943, 2021, 2030, 2237, 2430
StyD4I	CCNGG	5 cut(s)	1257, 1476, 1622, 1809, 2664
StyI	CCWWGG	4 cut(s)	83, 144, 264, 2082
TaaI	ACNGT	6 cut(s)	115, 757, 973, 1862, 2382, 2626
TaiI	ACGT	2 cut(s)	800, 904
TaqI	TCGA	5 cut(s)	1216, 1276, 1779, 2278, 2768
TaqII	GACCGA	2 cut(s)	1149, 1294
TatI	WGTACW	5 cut(s)	401, 1125, 1223, 1470, 2710
TauI	GCSGC	1 cut(s)	2438
Tru1I	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
Tru9I	TTAA	8 cut(s)	276, 450, 981, 1076, 1236, 1632, 2343, 2718
TscAI	CASTG	8 cut(s)	120, 172, 546, 634, 978, 1450, 1867, 1993
TseFI	GTSAC	4 cut(s)	163, 442, 590, 1445
TseI	GCWGC	2 cut(s)	2264, 2354
Tsp45I	GTSAC	4 cut(s)	163, 442, 590, 1445
TspGWI	ACGGA	1 cut(s)	2714
TspRI	CASTG	8 cut(s)	120, 172, 546, 634, 978, 1450, 1867, 1993
XagI	CCTNNNNNAGG	2 cut(s)	2336, 2510
XapI	RAATTY	6 cut(s)	191, 532, 963, 1181, 1239, 2047
XbaI	TCTAGA	1 cut(s)	2429
XceI	RCATGY	3 cut(s)	1056, 2081, 2486
XcmI	CCANNNNNNNNNTGG	1 cut(s)	81
XmiI	GTMKAC	2 cut(s)	249, 2039
XspI	CTAG	7 cut(s)	515, 918, 1943, 2021, 2030, 2237, 2430
ZrmI	AGTACT	1 cut(s)	1225
Zsp2I	ATGCAT	1 cut(s)	1543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.