Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0001221

Physical Location & Seq

Genomic Coordinates Forward (+)

4333015 .. 4340906

Gene Structure 7892 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016153

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2694 bp

ATGCCTCTCAGGCTTGACATTAAGCGGAAGTTTGCGCAAAGATCCGAGAGAGTGAAGTCTTTGGATCTGCATCCAACTGAACCATGGATCCTTGCAAGTCTTTACTCAGGAACTGTGTATATCTTGAACTACCAGTCACAGACCATGGCAAAGTCATTTGAAGTCTCTGAATTGCCAGTTCGGTCAGCAAAATTCGTAACACGAAAGCAATGGGTAGTTGCTGCAGCTGATGACATGTGTATACGTGTTTACAATTACAATACCATGGACAAGGTTAAAGTCTTTGAAGCACACAGTGACTACATTAGATGTGTGGCCGTCCATCCAACACTTCCATATGTTTTGTCATCATCTGATGACATGCTTATCAAACTGTGGGATTGGGAAAAGGGGTGGATGTGTACTCAGATATTTGAAGGGCATTCTCACTATGTCATGCAAGTGACATTTAATCCCAAAGACACCAACACTTTTGCAAGTGCTTCCCTTGATCACACTGTAAAGATTTGGAATCTTGTCTCCCCTGACCCAAATTTTACATTGGATGCCCACCTGAAAGGGGTCAATTGTGTTGATTACTTCACTGGTGGTGATAAACCGTACTTAATCACGGGATCTGATGATCACACTGCAAAGGTATGGGACTATCAAACAAAAAGTTGTGTTCAGACACTTGAAGGACACACTCATAATGTATCTGCAGTATGCTTCCACCCTGAACTTCCAGTTATAATTACTGGTTCTGAGGATGGAACTGTTAGAATATGGCATTCAACCACTTACAGGCTTGAAAACACCTTGAATTATGGGCTGGAACGTGTTTGGGCTTTTGGGTATATGAAAGGATCACGTCGGATTGTTATTGGTTATGACGAAGGAACCATTTTGGTAAAAATTGGTCGAGAGGTACCGGTTGCTAGCATGGATAGTAGTGGGAAAATAATCTGGGCCAAGCATAATGAAATCCAAACTGTGAATATCAAAAGTGTGGGATCGGATTTTGAGATTACAGATGGAGAAAGATTACCATTGGCCGTGAAGGAGCTGGGAACCTGTGATCTTTATCCTCAAAATTTGAAGCACAACCCCAATGGAAGGTTTGTTGTTGTTTGTGGTGATGGAGAGTACATAATATACACAGCTCTAGCATGGAGAAATAGATCCTTTGGCTCAGCATTGGAGTTTGTCTGGTCATCAGATGGAGAATATGCTGTTAGGGAAACAACATCAAAGATCAAAATTTTCAGCAAAACATTCCAGGAAAAGAAGAATATCCGACCAACATTTTCTGTTGAACACATTTATGGAGGGACACTATTGGCAATGTGTTCAAATGACTTTGTATGCTTCTATGAGTGGGGGGAATGCAGATTGATTAGACGAATTGATGTCAATGTTAAGAATGTTTATTGGGCTGATAGCGGTGATTTGGTGGCAATTTCTAGTGATTCATCATTCTACATTCTAAAATACGATAGGGATGTAGTCTCTTCTTATTTTGACAGTGGAAGCCCAGTTGATGAACTAGGTGTTGAGGATGCTTTTGAGCTGCTTTTTGAGATAAACGAGCGTGTCCGAACTGGATTATGGGTTGGCGACTGTTTCGTTTATAATAACTCCTCCTGGCGACTCAATTACTGTGTTGGTGGTGAGGTGACCACAATGTTTCATCTGGACCGGCCCATGTACTTGTTGGGATATCTTGCCAATCAAAGTCGTCTTTTTCTTATTGATAAAGAGTTCAATGTCATGGGATACTCCTTGCTTCTCAGCTTGATTGAGTACAAAACACTTGTTATGCGTGGAGAATTAGAGCGTGCCAAACAAATTTTTCCAACAATTCCCCAAGAGCAACATAACAGTGTGGCTCGATTTTTGGAATCTCGAGGGATGTTGGAGGATGCACTGAAAGTGGCTACAGATCCTGACTACAAATTTGATCTTGCTATACAGTTGGGTAATCTGGAGATTGCAAAGGAAATTGCTACTAAAGCAGAGAGTGAATCCAAATGGAAGCAGCTGGGAGATTTAGCTATGTCCTCTGGGAAGTTAGAACTTGCTGAAGATTGCCTATTATATGGAATGGACTTTTCTGGCTTGCTACTCCTGTATTCTTCTCTTGGAGATGCTCAAGGAATATCAAAGCTTGCTTCAATTGCTAAAGAGCAGGGAAAGAATAATGTTGCATTCCTTTGTTTGTTCTTGCTGGGTAAATTGGAAGAATGCATCCAGCTGTTGCTAGATAGTGAGCGGATACCTGAAGCAGCTTTGATGGCACGATCTTACCTACCCAGCAAGGTGTCAGAGATAGTTTCAATTTGGAAAAATGACCTGAACAAGGTTAATAAAAGAGCTGCAGAATCTTTGGCAGATCCCCAAGAATATCCTAATTTGTTTGAGGACTGGCAGATTTCTCTTTCCCTTGAGTCTAAACGCACTGAAAATAGTGGCAATTATCAACCAGCTGAGCAGTATCCAGTCTATGCTTCTGAGAAGCCAGCCAGCAGTCTTGTTGAATTGTTCAAAAGCATGCACATTGATGAAGAAGAAGCCGCCCCAGTTGAAAATGGAGATCTGGATCATGAGGAGGTAGAGGAGAATGGAGAAGATCAAGGTGAAGTACATACCATTGAGGAGGACTCAGACTCCATGAATGGTGTTGTTCTTGTGAATGGCACCCAGGAGGAAGAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005798 GO:0006810 GO:0006886 GO:0006888 GO:0006890 GO:0006891 GO:0008104 GO:0008150 GO:0012505 GO:0012506 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0030117 GO:0030120 GO:0030126 GO:0030135 GO:0030137 GO:0030659 GO:0030660 GO:0030662 GO:0030663 GO:0031090 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0048193 GO:0048475 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098588 GO:0098796 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Coatomer_WDAD WD40

Protein Analysis

898

Amino Acids

101.67

Weight (kDa)

4.95

Isoelectric Point (pI)

39.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	10 - 155	1.6e-14	WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	12 - 150	3.3e-11	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	13 - 131	1e-10	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5	PF25175	14 - 153	2.7e-19	WDR5 beta-propeller domain
WD40_Prp19	PF24814	16 - 184	4.1e-20	Prp19 WD40 domain
WDR55	PF24796	16 - 198	2.9e-15	WDR55
Beta-prop_WDR36-Utp21_2nd	PF25168	21 - 174	1.1e-09	WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3	PF25174	21 - 191	4.1e-23	THOC3 beta-propeller domain
WD40_CDC20-Fz	PF24807	35 - 193	5.1e-16	CDC20/Fizzy WD40 domain
Beta-prop_EIPR1	PF23609	53 - 170	6.2e-08	EIPR1 beta-propeller
WD40_WDHD1_1st	PF24817	58 - 131	3.3e-11	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	59 - 195	6.6e-27	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	67 - 211	2.3e-12	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	68 - 172	1.4e-08	MABP1/WDR62 second WD40 domain
Beta-prop_TEP1_2nd	PF25047	69 - 260	7.1e-13	TEP-1 second beta-propeller
Beta-prop_EML	PF23409	76 - 265	4.3e-15	Echinoderm microtubule-associated protein first beta-propeller
WD40	PF00400	91 - 127	5.3e-08	WD domain, G-beta repeat
WD40	PF00400	133 - 171	1e-06	WD domain, G-beta repeat
Beta-prop_WDR3_1st	PF25173	135 - 295	2.5e-27	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	135 - 245	5.7e-06	RIG second beta-propeller
EIF3I	PF24805	137 - 228	3.1e-06	EIF3I
WDR55	PF24796	139 - 296	6.7e-14	WDR55
Beta-prop_WDR3_2nd	PF25172	140 - 260	5e-14	WDR3 second beta-propeller domain
Beta-prop_WDR5	PF25175	154 - 288	7e-29	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	154 - 259	2.4e-11	G protein beta WD-40 repeat protein
Beta-prop_WDR36-Utp21_1st	PF25171	164 - 256	5.8e-06	WDR36/Utp21 first beta-propeller
WD40	PF00400	177 - 215	2.2e-06	WD domain, G-beta repeat
WD40_Prp19	PF24814	184 - 265	2.7e-15	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	186 - 295	2.6e-15	THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1	PF24105	196 - 265	1.1e-06	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	198 - 270	1.6e-08	CDC20/Fizzy WD40 domain
Beta-prop_WDR75_1st	PF23869	198 - 261	2.3e-06	WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st	PF24817	198 - 322	3e-12	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	199 - 296	4.2e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd	PF23393	202 - 258	2.7e-07	WDR90/POC16, second beta-propeller
Beta-prop_SCAP	PF24017	202 - 259	4.4e-06	SCAP Beta-propeller
Beta-prop_WDR36-Utp21_2nd	PF25168	206 - 261	8e-07	WDR36/Utp21 second beta-propeller domain
WD40	PF00400	220 - 256	2.5e-11	WD domain, G-beta repeat
Beta-prop_EML_2	PF23414	234 - 298	8.5e-06	Echinoderm microtubule-associated protein second beta-propeller
B-prop_COPA_B_2nd	PF04053	319 - 578	4.5e-86	COPA/B second beta-propeller
TPR_COPA_B	PF23953	595 - 775	2.8e-80	COPA/B TPR domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0001221)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52360 AT1G52360 AT1G52360 AT1G52360 AT1G79990 AT1G79990 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980 AT3G15980
fragaria_vesca	FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_1g05170 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010 FvH4_6g31010
malus_domestica	MD09G1214300.v1.1 MD15G1188300.v1.1 MD17G1196600.v1.1
prunus_persica	Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.3G072300_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1 Prupe.7G228200_v2.0.a1
pyrus_communis	pycom02g04220 pycom09g13110 pycom15g16880 pycom17g20170
rosa_chinensis	RchiOBHm_Chr2g0090881 RchiOBHm_Chr2g0137661
rosa_laevigata	RLG00000016153 RLG00000019667
rosa_multiflora	Rmu_sc0000765.1_g000033 Rmu_sc0000792.1_g000012
rosa_roxburghii	Rroxscaffold_2G00106960 Rroxscaffold_2G00150770
rosa_rugosa	Rorug02G0010700 Rorug02G0010800 Rorug02G0343900
rosa_samantha	Rh2AG057000 Rh2AG391700 Rh2BG399200 Rh2CG378600 Rh2DG056400 Rh2DG414300
rosa_wichuraiana	Rw2G005240 Rw2G032090

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	731, 1611
Acc16I	TGCGCA	1 cut(s)	36
Acc65I	GGTACC	1 cut(s)	907
AccB1I	GGYRCC	2 cut(s)	907, 2675
AccBSI	CCGCTC	1 cut(s)	2251
AccI	GTMKAC	1 cut(s)	241
AciI	CCGC	4 cut(s)	25, 1422, 2251, 2553
AcoI	YGGCCR	2 cut(s)	315, 1032
AcsI	RAATTY	6 cut(s)	191, 532, 1072, 1239, 1827, 1934
AcuI	CTGAAG	2 cut(s)	2082, 2280
AdeI	CACNNNGTG	1 cut(s)	296
AfaI	GTAC	7 cut(s)	403, 602, 909, 1127, 1688, 1784, 2622
AfiI	CCNNNNNNNGG	4 cut(s)	559, 783, 1095, 2338
AflIII	ACRYGT	3 cut(s)	234, 244, 817
AgeI	ACCGGT	1 cut(s)	910
AjiI	CACGTC	1 cut(s)	851
AjnI	CCWGG	3 cut(s)	1257, 1622, 2679
AloI	GAACNNNNNNTCC	2 cut(s)	72, 104
Alw26I	GTCTC	3 cut(s)	169, 523, 1492
AlwNI	CAGNNNCTG	2 cut(s)	113, 1925
Ama87I	CYCGRG	1 cut(s)	1884
AoxI	GGCC	4 cut(s)	315, 948, 1032, 1679
ApeKI	GCWGC	6 cut(s)	221, 224, 1549, 2017, 2264, 2354
ApoI	RAATTY	6 cut(s)	191, 532, 1072, 1239, 1827, 1934
ArsI	GACNNNNNNTTYG	2 cut(s)	1919, 1951
AsiGI	ACCGGT	1 cut(s)	910
Asp718I	GGTACC	1 cut(s)	907
AspLEI	GCGC	1 cut(s)	37
AspS9I	GGNCC	3 cut(s)	948, 1675, 1680
AsuHPI	GGTGA	6 cut(s)	602, 1127, 1436, 1661, 1666, 2627
AsuNHI	GCTAGC	1 cut(s)	917
AvaI	CYCGRG	1 cut(s)	1884
AvaII	GGWCC	1 cut(s)	1675
BamHI	GGATCC	1 cut(s)	87
BanI	GGYRCC	2 cut(s)	907, 2675
BbvI	GCAGC	6 cut(s)	208, 236, 1536, 2029, 2276, 2341
BccI	CCATC	6 cut(s)	330, 743, 1007, 1112, 1193, 2266
BceAI	ACGGC	2 cut(s)	302, 1019
BciT130I	CCWGG	3 cut(s)	1259, 1624, 2681
BciVI	GTATCC	3 cut(s)	1748, 2247, 2484
BclI	TGATCA	2 cut(s)	490, 622
BcoDI	GTCTC	3 cut(s)	169, 523, 1492
BfaI	CTAG	5 cut(s)	918, 1145, 1443, 1526, 2240
BfmI	CTRYAG	4 cut(s)	222, 699, 1917, 2355
BfuI	GTATCC	3 cut(s)	1748, 2247, 2484
BglI	GCCNNNNNGGC	1 cut(s)	10
BglII	AGATCT	1 cut(s)	2572
BisI	GCNGC	7 cut(s)	222, 225, 1550, 2018, 2265, 2355, 2553
BlpI	GCTNAGC	2 cut(s)	1171, 2466
BlsI	GCNGC	7 cut(s)	223, 226, 1551, 2019, 2266, 2356, 2554
Bme1390I	CCNGG	3 cut(s)	1259, 1624, 2681
Bme18I	GGWCC	1 cut(s)	1675
BmeT110I	CYCGRG	1 cut(s)	1884
BmgBI	CACGTC	1 cut(s)	851
BmgT120I	GGNCC	3 cut(s)	948, 1675, 1680
BmiI	GGNNCC	5 cut(s)	89, 880, 909, 1051, 2677
BmrFI	CCNGG	3 cut(s)	1259, 1624, 2681
BmrI	ACTGGG	2 cut(s)	1508, 2552
BmsI	GCATC	6 cut(s)	79, 535, 1528, 1891, 2116, 2235
BmtI	GCTAGC	1 cut(s)	921
BmuI	ACTGGG	2 cut(s)	1508, 2552
BplI	GAGNNNNNCTC	2 cut(s)	2624, 2656
BpmI	CTGGAG	1 cut(s)	1985
Bpu1102I	GCTNAGC	2 cut(s)	1171, 2466
BpuEI	CTTGAG	2 cut(s)	2115, 2444
BsaAI	YACGTR	1 cut(s)	245
BsaBI	GATNNNNATC	3 cut(s)	69, 1062, 2577
BsaJI	CCNNGG	4 cut(s)	83, 144, 264, 2679
BsaWI	WCCGGW	1 cut(s)	910
BsaXI	ACNNNNNCTCC	3 cut(s)	2630, 2660, 2675
Bsc4I	CCNNNNNNNGG	4 cut(s)	559, 783, 1095, 2338
Bse118I	RCCGGY	2 cut(s)	910, 1677
Bse3DI	GCAATG	2 cut(s)	215, 1329
Bse8I	GATNNNNATC	3 cut(s)	69, 1062, 2577
BseBI	CCWGG	3 cut(s)	1259, 1624, 2681
BseDI	CCNNGG	4 cut(s)	83, 144, 264, 2679
BseJI	GATNNNNATC	3 cut(s)	69, 1062, 2577
BseLI	CCNNNNNNNGG	4 cut(s)	559, 783, 1095, 2338
BseMI	GCAATG	2 cut(s)	215, 1329
BseMII	CTCAG	9 cut(s)	22, 120, 419, 735, 1185, 1783, 2457, 2481, 2655
BseRI	GAGGAG	4 cut(s)	1609, 2600, 2609, 2648
BseXI	GCAGC	6 cut(s)	208, 236, 1536, 2029, 2276, 2341
BseYI	CCCAGC	4 cut(s)	1045, 2020, 2206, 2291
BshFI	GGCC	4 cut(s)	317, 950, 1034, 1681
BshNI	GGYRCC	2 cut(s)	907, 2675
BshTI	ACCGGT	1 cut(s)	910
BsiHKCI	CYCGRG	1 cut(s)	1884
BsiSI	CCGG	2 cut(s)	911, 1678
BslFI	GGGAC	2 cut(s)	656, 1324
BslI	CCNNNNNNNGG	4 cut(s)	559, 783, 1095, 2338
BsmAI	GTCTC	3 cut(s)	169, 523, 1492
BsmFI	GGGAC	2 cut(s)	656, 1324
BsmI	GAATGC	5 cut(s)	421, 769, 1370, 2186, 2228
BsnI	GGCC	4 cut(s)	317, 950, 1034, 1681
BsoBI	CYCGRG	1 cut(s)	1884
Bsp1720I	GCTNAGC	2 cut(s)	1171, 2466
Bsp19I	CCATGG	3 cut(s)	83, 144, 264
BspACI	CCGC	4 cut(s)	25, 1422, 2251, 2553
BspANI	GGCC	4 cut(s)	317, 950, 1034, 1681
BspCNI	CTCAG	9 cut(s)	21, 119, 418, 736, 1184, 1782, 2458, 2482, 2654
BspHI	TCATGA	1 cut(s)	2581
BspLI	GGNNCC	5 cut(s)	89, 880, 909, 1051, 2677
BspMAI	CTGCAG	3 cut(s)	226, 703, 2359
BspOI	GCTAGC	1 cut(s)	921
BspT107I	GGYRCC	2 cut(s)	907, 2675
BsrBI	CCGCTC	1 cut(s)	2251
BsrDI	GCAATG	2 cut(s)	215, 1329
BsrFI	RCCGGY	2 cut(s)	910, 1677
BssAI	RCCGGY	2 cut(s)	910, 1677
BssECI	CCNNGG	4 cut(s)	83, 144, 264, 2679
BssNAI	GTATAC	1 cut(s)	242
BssT1I	CCWWGG	3 cut(s)	83, 144, 264
Bst1107I	GTATAC	1 cut(s)	242
Bst2UI	CCWGG	3 cut(s)	1259, 1624, 2681
Bst6I	CTCTTC	2 cut(s)	1495, 2682
BstBAI	YACGTR	1 cut(s)	245
BstC8I	GCNNGC	7 cut(s)	919, 1818, 2099, 2148, 2499, 2503, 2531
BstDEI	CTNAG	9 cut(s)	8, 106, 405, 744, 1171, 1769, 2466, 2490, 2641
BstDSI	CCRYGG	3 cut(s)	83, 144, 264
BstEII	GGTNACC	1 cut(s)	1654
BstENI	CCTNNNNNAGG	1 cut(s)	2336
BstHHI	GCGC	1 cut(s)	37
BstMAI	GTCTC	3 cut(s)	169, 523, 1492
BstMWI	GCNNNNNNNGC	4 cut(s)	10, 1991, 2156, 2273
BstNI	CCWGG	3 cut(s)	1259, 1624, 2681
BstNSI	RCATGY	3 cut(s)	238, 364, 2533
BstPI	GGTNACC	1 cut(s)	1654
BstSCI	CCNGG	3 cut(s)	1257, 1622, 2679
BstSFI	CTRYAG	4 cut(s)	222, 699, 1917, 2355
BstV1I	GCAGC	6 cut(s)	208, 236, 1536, 2029, 2276, 2341
BstX2I	RGATCY	8 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572
BstYI	RGATCY	8 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572
BstZ17I	GTATAC	1 cut(s)	242
BsuI	GTATCC	3 cut(s)	1748, 2247, 2484
BsuRI	GGCC	4 cut(s)	317, 950, 1034, 1681
BtgI	CCRYGG	3 cut(s)	83, 144, 264
BtrI	CACGTC	1 cut(s)	851
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	8 cut(s)	301, 495, 582, 627, 1510, 1867, 1904, 2436
Cac8I	GCNNGC	7 cut(s)	919, 1818, 2099, 2148, 2499, 2503, 2531
CaiI	CAGNNNCTG	2 cut(s)	113, 1925
CciI	TCATGA	1 cut(s)	2581
CfoI	GCGC	1 cut(s)	37
Cfr10I	RCCGGY	2 cut(s)	910, 1677
Cfr13I	GGNCC	3 cut(s)	948, 1675, 1680
Csp6I	GTAC	7 cut(s)	402, 601, 908, 1126, 1687, 1783, 2621
CspAI	ACCGGT	1 cut(s)	910
CviQI	GTAC	7 cut(s)	402, 601, 908, 1126, 1687, 1783, 2621
DdeI	CTNAG	9 cut(s)	8, 106, 405, 744, 1171, 1769, 2466, 2490, 2641
DraIII	CACNNNGTG	1 cut(s)	296
EaeI	YGGCCR	2 cut(s)	315, 1032
Eam1104I	CTCTTC	2 cut(s)	1495, 2682
EarI	CTCTTC	2 cut(s)	1495, 2682
Eco130I	CCWWGG	3 cut(s)	83, 144, 264
Eco32I	GATATC	1 cut(s)	1700
Eco47I	GGWCC	1 cut(s)	1675
Eco57I	CTGAAG	2 cut(s)	2082, 2280
Eco88I	CYCGRG	1 cut(s)	1884
Eco91I	GGTNACC	1 cut(s)	1654
EcoNI	CCTNNNNNAGG	1 cut(s)	2336
EcoO65I	GGTNACC	1 cut(s)	1654
EcoRII	CCWGG	3 cut(s)	1257, 1622, 2679
EcoRV	GATATC	1 cut(s)	1700
EcoT14I	CCWWGG	3 cut(s)	83, 144, 264
EcoT22I	ATGCAT	1 cut(s)	2228
ErhI	CCWWGG	3 cut(s)	83, 144, 264
FaqI	GGGAC	2 cut(s)	656, 1324
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	2 cut(s)	490, 622
FblI	GTMKAC	1 cut(s)	241
Fnu4HI	GCNGC	7 cut(s)	222, 225, 1550, 2018, 2265, 2355, 2553
Fsp4HI	GCNGC	7 cut(s)	222, 225, 1550, 2018, 2265, 2355, 2553
FspBI	CTAG	5 cut(s)	918, 1145, 1443, 1526, 2240
FspI	TGCGCA	1 cut(s)	36
GlaI	GCGC	1 cut(s)	36
GluI	GCNGC	7 cut(s)	222, 225, 1550, 2018, 2265, 2355, 2553
GsaI	CCCAGC	4 cut(s)	1049, 2024, 2210, 2295
GsuI	CTGGAG	1 cut(s)	1985
HaeIII	GGCC	4 cut(s)	317, 950, 1034, 1681
HapII	CCGG	2 cut(s)	911, 1678
HhaI	GCGC	1 cut(s)	37
Hin6I	GCGC	1 cut(s)	35
HinP1I	GCGC	1 cut(s)	35
HindIII	AAGCTT	1 cut(s)	2144
HinfI	GANTC	9 cut(s)	511, 1448, 1629, 1880, 2003, 2360, 2426, 2639, 2645
HpaII	CCGG	2 cut(s)	911, 1678
HphI	GGTGA	6 cut(s)	602, 1127, 1436, 1661, 1666, 2627
Hpy166II	GTNNAC	3 cut(s)	242, 250, 402
Hpy188III	TCNNGA	9 cut(s)	108, 124, 902, 1673, 1884, 1925, 1964, 2576, 2582
Hpy8I	GTNNAC	3 cut(s)	242, 250, 402
Hpy99I	CGWCG	1 cut(s)	855
HpyAV	CCTTC	5 cut(s)	410, 671, 869, 1033, 1089
HpyCH4IV	ACGT	3 cut(s)	244, 817, 850
HpyF10VI	GCNNNNNNNGC	4 cut(s)	10, 1991, 2156, 2273
HpyF3I	CTNAG	9 cut(s)	8, 106, 405, 744, 1171, 1769, 2466, 2490, 2641
HpySE526I	ACGT	3 cut(s)	244, 817, 850
HspAI	GCGC	1 cut(s)	35
KpnI	GGTACC	1 cut(s)	911
Ksp22I	TGATCA	2 cut(s)	490, 622
LmnI	GCTCC	1 cut(s)	1042
Lsp1109I	GCAGC	6 cut(s)	208, 236, 1536, 2029, 2276, 2341
LweI	GCATC	6 cut(s)	79, 535, 1528, 1891, 2116, 2235
MaeI	CTAG	5 cut(s)	918, 1145, 1443, 1526, 2240
MaeII	ACGT	3 cut(s)	244, 817, 850
MaeIII	GTNAC	5 cut(s)	135, 196, 296, 442, 1654
MbiI	CCGCTC	1 cut(s)	2251
MboII	GAAGA	8 cut(s)	1279, 1482, 2075, 2106, 2231, 2555, 2558, 2618
MfeI	CAATTG	2 cut(s)	565, 2154
MflI	RGATCY	8 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572
MlyI	GAGTC	4 cut(s)	1623, 2435, 2633, 2639
MmeI	TCCRAC	6 cut(s)	98, 350, 833, 1300, 1859, 1875
Mph1103I	ATGCAT	1 cut(s)	2228
MseI	TTAA	6 cut(s)	21, 276, 450, 605, 1398, 2343
MslI	CAYNNNNRTG	4 cut(s)	440, 1302, 1860, 2538
MspA1I	CMGCKG	4 cut(s)	227, 2020, 2233, 2465
MspI	CCGG	2 cut(s)	911, 1678
MspR9I	CCNGG	3 cut(s)	1259, 1624, 2681
MunI	CAATTG	2 cut(s)	565, 2154
Mva1269I	GAATGC	5 cut(s)	421, 769, 1370, 2186, 2228
MvaI	CCWGG	3 cut(s)	1259, 1624, 2681
MwoI	GCNNNNNNNGC	4 cut(s)	10, 1991, 2156, 2273
NcoI	CCATGG	3 cut(s)	83, 144, 264
NdeI	CATATG	1 cut(s)	337
NheI	GCTAGC	1 cut(s)	917
NlaIV	GGNNCC	5 cut(s)	89, 880, 909, 1051, 2677
NmuCI	GTSAC	4 cut(s)	135, 296, 442, 1654
NsbI	TGCGCA	1 cut(s)	36
NsiI	ATGCAT	1 cut(s)	2228
NspI	RCATGY	3 cut(s)	238, 364, 2533
PaeI	GCATGC	1 cut(s)	2533
PaeR7I	CTCGAG	1 cut(s)	1884
PagI	TCATGA	1 cut(s)	2581
PciI	ACATGT	1 cut(s)	234
PctI	GAATGC	5 cut(s)	421, 769, 1370, 2186, 2228
PfeI	GAWTC	5 cut(s)	511, 1448, 1880, 2003, 2360
PfoI	TCCNGGA	1 cut(s)	1257
PinAI	ACCGGT	1 cut(s)	910
PkrI	GCNGC	7 cut(s)	223, 226, 1551, 2019, 2266, 2356, 2554
PleI	GAGTC	4 cut(s)	1623, 2434, 2633, 2639
PpsI	GAGTC	4 cut(s)	1623, 2434, 2633, 2639
Ppu21I	YACGTR	1 cut(s)	245
PscI	ACATGT	1 cut(s)	234
PsiI	TTATAA	2 cut(s)	731, 1611
Psp6I	CCWGG	3 cut(s)	1257, 1622, 2679
PspEI	GGTNACC	1 cut(s)	1654
PspFI	CCCAGC	4 cut(s)	1045, 2020, 2206, 2291
PspGI	CCWGG	3 cut(s)	1257, 1622, 2679
PspN4I	GGNNCC	5 cut(s)	89, 880, 909, 1051, 2677
PspPI	GGNCC	3 cut(s)	948, 1675, 1680
PstI	CTGCAG	3 cut(s)	226, 703, 2359
PstNI	CAGNNNCTG	2 cut(s)	113, 1925
PsuI	RGATCY	8 cut(s)	41, 64, 87, 614, 1160, 1921, 2371, 2572
PvuII	CAGCTG	4 cut(s)	227, 2020, 2233, 2465
RsaI	GTAC	7 cut(s)	403, 602, 909, 1127, 1688, 1784, 2622
RsaNI	GTAC	7 cut(s)	402, 601, 908, 1126, 1687, 1783, 2621
RseI	CAYNNNNRTG	4 cut(s)	440, 1302, 1860, 2538
SaqAI	TTAA	6 cut(s)	21, 276, 450, 605, 1398, 2343
SatI	GCNGC	7 cut(s)	222, 225, 1550, 2018, 2265, 2355, 2553
Sau96I	GGNCC	3 cut(s)	948, 1675, 1680
SchI	GAGTC	4 cut(s)	1623, 2435, 2633, 2639
ScrFI	CCNGG	3 cut(s)	1259, 1624, 2681
SfaNI	GCATC	6 cut(s)	79, 535, 1528, 1891, 2116, 2235
SfcI	CTRYAG	4 cut(s)	222, 699, 1917, 2355
Sfr274I	CTCGAG	1 cut(s)	1884
SinI	GGWCC	1 cut(s)	1675
SlaI	CTCGAG	1 cut(s)	1884
SmiMI	CAYNNNNRTG	4 cut(s)	440, 1302, 1860, 2538
SmlI	CTYRAG	3 cut(s)	1884, 2130, 2423
SmoI	CTYRAG	3 cut(s)	1884, 2130, 2423
SphI	GCATGC	1 cut(s)	2533
SsiI	CCGC	4 cut(s)	25, 1422, 2251, 2553
SspMI	CTAG	5 cut(s)	918, 1145, 1443, 1526, 2240
StyD4I	CCNGG	3 cut(s)	1257, 1622, 2679
StyI	CCWWGG	3 cut(s)	83, 144, 264
TaiI	ACGT	3 cut(s)	247, 820, 853
TaqI	TCGA	3 cut(s)	901, 1870, 1885
TaqII	GACCGA	1 cut(s)	171
TatI	WGTACW	5 cut(s)	401, 1125, 1686, 1782, 2620
TauI	GCSGC	1 cut(s)	2555
TfiI	GAWTC	5 cut(s)	511, 1448, 1880, 2003, 2360
Tru1I	TTAA	6 cut(s)	21, 276, 450, 605, 1398, 2343
Tru9I	TTAA	6 cut(s)	21, 276, 450, 605, 1398, 2343
TscAI	CASTG	8 cut(s)	301, 502, 589, 634, 1510, 1867, 1911, 2443
TseFI	GTSAC	4 cut(s)	135, 296, 442, 1654
TseI	GCWGC	6 cut(s)	221, 224, 1549, 2017, 2264, 2354
Tsp45I	GTSAC	4 cut(s)	135, 296, 442, 1654
TspDTI	ATGAA	7 cut(s)	854, 975, 1440, 1536, 1658, 2556, 2666
TspRI	CASTG	8 cut(s)	301, 502, 589, 634, 1510, 1867, 1911, 2443
VpaK11BI	GGWCC	1 cut(s)	1675
XagI	CCTNNNNNAGG	1 cut(s)	2336
XapI	RAATTY	6 cut(s)	191, 532, 1072, 1239, 1827, 1934
XceI	RCATGY	3 cut(s)	238, 364, 2533
XcmI	CCANNNNNNNNNTGG	2 cut(s)	81, 1690
XhoI	CTCGAG	1 cut(s)	1884
XmiI	GTMKAC	1 cut(s)	241
XspI	CTAG	5 cut(s)	918, 1145, 1443, 1526, 2240
Zsp2I	ATGCAT	1 cut(s)	2228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.