AT1G53250

NUMOD3 motif (2 copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002997
Physical Location & Seq
Forward (+)
19857351 .. 19859275
1925 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G53250.1

Sequence Viewer

Length: 1116 bp
ATGTCACTAAATTTAGGTTTATCAATGGAGATGAAATGCACATTTCAATCAACTCGTGCTCCATTCCAATGCGCTTGGTGGTTCCCAAATTCAATCTATTGGACTCTCAAAGAACCAATCGTTTTGAGAATACCTAATTCACTTGCTTCTTTGAGGTCTATCAGACACTTGGAGTTGAAATCTGTAGGTTCATTGTACAATGTGTTTGAGATTCATAGGAAAGAAGTCAATTCAAGTCTTTTGGAAGTGAAAGCTATGAACAAAGATACTGAAGCTGATAGTGATAGTGATAGGAAGATTAAAGAAGAGGAAAGGAGAAGGAAGATTGGATTAGCTAATAAAGGAAAGGTGCCATGGAACAAAGGAAGGAAACACAGTGAAGACACTCGAAGACGAATCAAGCAGAGAACAATCGAAGCTTTGACAAATCCCAAGGTTCGGAAGAAGATGTCCGATCATCAACAACCACACAGTAATGAAACCAAGGAGAAGATAAGAGCTTCAGTGAAACAAGTTTGGGCAGAACGGTCAAGATCGAAGCGATTAAAGGAGAAGTTCATGTCATCGTGGTCAGAAAACATTGCAGAAGCTGCAAGGAAAGGAGGAAGTGGCGAGGCAGAACTTGACTGGGACAGCTATGAAAAAATAAAACAAGATTTTTCATCTGAGCAGCTTCAGTTAGCTGAAGAGAAAGCAAGAGCTAAGGAACAAACCAAGATGATAGCAAAAGAAGCTGCAAAAGCCAGGACCGAGAAGATGAGGAGAGCTGCAGAAAAAAAGAAAGAACGTGAGGAGAAAGACCGACGAGAAGGAAAGATTCGAAAGCCAAAGCAGGAAAGGGAGAATCCAACCATTGCTTCACGTTCTAAACTAAAGAAGAGACTAACAAAGATTCACAAGAAGAAAACAAGTCTTGGTAAGATCGCAATTGGAACGGATAGGGTTGTTTCAGTTGCAGCTAAACTGGAGAAACTGGATTTGGATTTGATAAGGAAAGAGCGAACAAGAGGAGATATCTCACTTGCTGATCAGATCCAAGCTGCTAAGAACCAACGAGGAAGTGATGTTTTATCGAGATTTGGTCTTTTTGCCATGAAATCGATGGATTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NUMOD3

Protein Analysis

371

Amino Acids

43.03

Weight (kDa)

10.13

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUMOD3 PF07460 103 - 133 2.4e-09 NUMOD3 motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 349
AclWI GGATC 1 cut(s) 1027
AcsI RAATTY 2 cut(s) 10, 88
AcuI CTGAAG 4 cut(s) 291, 486, 659, 705
AfaI GTAC 1 cut(s) 197
AfiI CCNNNNNNNGG 1 cut(s) 438
AgsI TTSAA 4 cut(s) 47, 93, 178, 234
AjnI CCWGG 1 cut(s) 743
AjuI GAANNNNNNNTTGG 4 cut(s) 841, 873, 962, 994
Alw21I GWGCWC 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 874
AlwI GGATC 1 cut(s) 1027
AlwNI CAGNNNCTG 1 cut(s) 590
ApeKI GCWGC 6 cut(s) 590, 670, 734, 767, 956, 1040
ApoI RAATTY 2 cut(s) 10, 88
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 747
AsuII TTCGAA 1 cut(s) 820
AvaII GGWCC 1 cut(s) 747
BanI GGYRCC 1 cut(s) 349
BauI CACGAG 1 cut(s) 54
BbsI GAAGAC 2 cut(s) 387, 397
Bbv12I GWGCWC 1 cut(s) 61
BbvI GCAGC 6 cut(s) 577, 682, 721, 754, 968, 1027
BccI CCATC 1 cut(s) 1096
BciT130I CCWGG 1 cut(s) 745
BclI TGATCA 1 cut(s) 1027
BcoDI GTCTC 1 cut(s) 874
BfmI CTRYAG 2 cut(s) 183, 768
BisI GCNGC 6 cut(s) 591, 671, 735, 768, 957, 1041
BlsI GCNGC 6 cut(s) 592, 672, 736, 769, 958, 1042
Bme1390I CCNGG 1 cut(s) 745
Bme18I GGWCC 1 cut(s) 747
BmgT120I GGNCC 1 cut(s) 747
BmiI GGNNCC 2 cut(s) 83, 351
BmrFI CCNGG 1 cut(s) 745
BmrI ACTGGG 1 cut(s) 637
BmuI ACTGGG 1 cut(s) 637
BpiI GAAGAC 2 cut(s) 387, 397
BpmI CTGGAG 1 cut(s) 986
Bpu10I CCTNAGC 1 cut(s) 702
Bpu14I TTCGAA 1 cut(s) 820
Bsa29I ATCGAT 1 cut(s) 1100
BsaJI CCNNGG 3 cut(s) 353, 432, 483
BsaXI ACNNNNNCTCC 2 cut(s) 43, 73
Bsc4I CCNNNNNNNGG 1 cut(s) 438
Bse1I ACTGG 3 cut(s) 632, 969, 978
Bse3DI GCAATG 2 cut(s) 579, 852
BseBI CCWGG 1 cut(s) 745
BseCI ATCGAT 1 cut(s) 1100
BseDI CCNNGG 3 cut(s) 353, 432, 483
BseLI CCNNNNNNNGG 1 cut(s) 438
BseMI GCAATG 2 cut(s) 579, 852
BseMII CTCAG 1 cut(s) 657
BseNI ACTGG 3 cut(s) 632, 969, 978
BseRI GAGGAG 3 cut(s) 775, 806, 1023
BseXI GCAGC 6 cut(s) 577, 682, 721, 754, 968, 1027
BshNI GGYRCC 1 cut(s) 349
BshVI ATCGAT 1 cut(s) 1100
BsiHKAI GWGCWC 1 cut(s) 61
BslFI GGGAC 1 cut(s) 644
BslI CCNNNNNNNGG 1 cut(s) 438
BsmAI GTCTC 1 cut(s) 874
BsmFI GGGAC 1 cut(s) 644
Bsp119I TTCGAA 1 cut(s) 820
Bsp1286I GDGCHC 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 195
Bsp143I GATC 5 cut(s) 454, 533, 921, 1027, 1032
Bsp19I CCATGG 1 cut(s) 353
BspCNI CTCAG 1 cut(s) 658
BspDI ATCGAT 1 cut(s) 1100
BspLI GGNNCC 2 cut(s) 83, 351
BspMAI CTGCAG 1 cut(s) 772
BspPI GGATC 1 cut(s) 1027
BspT104I TTCGAA 1 cut(s) 820
BspT107I GGYRCC 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 579, 852
BsrGI TGTACA 1 cut(s) 195
BsrI ACTGG 3 cut(s) 632, 969, 978
BssECI CCNNGG 3 cut(s) 353, 432, 483
BssMI GATC 5 cut(s) 454, 533, 921, 1027, 1032
BssSI CACGAG 1 cut(s) 54
BssT1I CCWWGG 3 cut(s) 353, 432, 483
Bst2BI CACGAG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 745
Bst4CI ACNGT 3 cut(s) 377, 473, 528
Bst6I CTCTTC 3 cut(s) 300, 681, 872
BstAPI GCANNNNNTGC 1 cut(s) 590
BstAUI TGTACA 1 cut(s) 195
BstBI TTCGAA 1 cut(s) 820
BstDEI CTNAG 3 cut(s) 666, 702, 1044
BstDSI CCRYGG 1 cut(s) 353
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 5 cut(s) 457, 536, 924, 1030, 1035
BstMAI GTCTC 1 cut(s) 874
BstMBI GATC 5 cut(s) 454, 533, 921, 1027, 1032
BstMWI GCNNNNNNNGC 3 cut(s) 590, 731, 740
BstNI CCWGG 1 cut(s) 745
BstSCI CCNGG 1 cut(s) 743
BstSFI CTRYAG 2 cut(s) 183, 768
BstV1I GCAGC 6 cut(s) 577, 682, 721, 754, 968, 1027
BstV2I GAAGAC 2 cut(s) 387, 397
BstX2I RGATCY 1 cut(s) 1032
BstYI RGATCY 1 cut(s) 1032
Bsu15I ATCGAT 1 cut(s) 1100
BsuTUI ATCGAT 1 cut(s) 1100
BtgI CCRYGG 1 cut(s) 353
BtsIMutI CAGTG 2 cut(s) 382, 510
CaiI CAGNNNCTG 1 cut(s) 590
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 747
ClaI ATCGAT 1 cut(s) 1100
Csp6I GTAC 1 cut(s) 196
CviAII CATG 3 cut(s) 354, 559, 1093
CviQI GTAC 1 cut(s) 196
DdeI CTNAG 3 cut(s) 666, 702, 1044
DpnI GATC 5 cut(s) 456, 535, 923, 1029, 1034
DpnII GATC 5 cut(s) 454, 533, 921, 1027, 1032
Eam1104I CTCTTC 3 cut(s) 300, 681, 872
EarI CTCTTC 3 cut(s) 300, 681, 872
Eco130I CCWWGG 3 cut(s) 353, 432, 483
Eco32I GATATC 1 cut(s) 1015
Eco47I GGWCC 1 cut(s) 747
Eco57I CTGAAG 4 cut(s) 291, 486, 659, 705
EcoRII CCWGG 1 cut(s) 743
EcoRV GATATC 1 cut(s) 1015
EcoT14I CCWWGG 3 cut(s) 353, 432, 483
ErhI CCWWGG 3 cut(s) 353, 432, 483
FaeI CATG 3 cut(s) 357, 562, 1096
FaiI YATR 6 cut(s) 216, 257, 355, 560, 639, 1094
FaqI GGGAC 1 cut(s) 644
FatI CATG 3 cut(s) 353, 558, 1092
FbaI TGATCA 1 cut(s) 1027
Fnu4HI GCNGC 6 cut(s) 591, 671, 735, 768, 957, 1041
Fsp4HI GCNGC 6 cut(s) 591, 671, 735, 768, 957, 1041
GlaI GCGC 1 cut(s) 73
GluI GCNGC 6 cut(s) 591, 671, 735, 768, 957, 1041
GsuI CTGGAG 1 cut(s) 986
HhaI GCGC 1 cut(s) 74
Hin1II CATG 3 cut(s) 357, 562, 1096
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 6 cut(s) 103, 211, 396, 817, 844, 892
Hpy188I TCNGA 6 cut(s) 164, 441, 454, 574, 667, 1032
Hpy188III TCNNGA 2 cut(s) 531, 1074
Hpy99I CGWCG 1 cut(s) 807
HpyAV CCTTC 3 cut(s) 312, 360, 803
HpyCH4III ACNGT 3 cut(s) 377, 473, 528
HpyCH4IV ACGT 2 cut(s) 787, 862
HpyCH4V TGCA 6 cut(s) 39, 584, 593, 737, 770, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 590, 731, 740
HpyF3I CTNAG 3 cut(s) 666, 702, 1044
HpySE526I ACGT 2 cut(s) 787, 862
Hsp92II CATG 3 cut(s) 357, 562, 1096
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 1027
Kzo9I GATC 5 cut(s) 454, 533, 921, 1027, 1032
LmnI GCTCC 1 cut(s) 64
LpnPI CCDG 6 cut(s) 613, 730, 757, 818, 950, 959
Lsp1109I GCAGC 6 cut(s) 577, 682, 721, 754, 968, 1027
MaeII ACGT 2 cut(s) 787, 862
MaeIII GTNAC 1 cut(s) 3
MalI GATC 5 cut(s) 456, 535, 923, 1029, 1034
MboI GATC 5 cut(s) 454, 533, 921, 1027, 1032
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 1 cut(s) 1032
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 5 cut(s) 10, 88, 136, 229, 927
MlyI GAGTC 1 cut(s) 97
MmeI TCCRAC 1 cut(s) 872
MnlI CCTC 8 cut(s) 147, 301, 596, 607, 753, 784, 1001, 1049
MseI TTAA 3 cut(s) 300, 545, 1114
MslI CAYNNNNRTG 2 cut(s) 67, 474
MspR9I CCNGG 1 cut(s) 745
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 1 cut(s) 745
MwoI GCNNNNNNNGC 3 cut(s) 590, 731, 740
NcoI CCATGG 1 cut(s) 353
NdeII GATC 5 cut(s) 454, 533, 921, 1027, 1032
NlaIII CATG 3 cut(s) 357, 562, 1096
NlaIV GGNNCC 2 cut(s) 83, 351
NmuCI GTSAC 1 cut(s) 3
NspV TTCGAA 1 cut(s) 820
PfeI GAWTC 5 cut(s) 211, 396, 817, 844, 892
PkrI GCNGC 6 cut(s) 592, 672, 736, 769, 958, 1042
PleI GAGTC 1 cut(s) 97
PpsI GAGTC 1 cut(s) 97
Psp6I CCWGG 1 cut(s) 743
PspGI CCWGG 1 cut(s) 743
PspN4I GGNNCC 2 cut(s) 83, 351
PspPI GGNCC 1 cut(s) 747
PstI CTGCAG 1 cut(s) 772
PstNI CAGNNNCTG 1 cut(s) 590
PsuI RGATCY 1 cut(s) 1032
RsaI GTAC 1 cut(s) 197
RsaNI GTAC 1 cut(s) 196
RseI CAYNNNNRTG 2 cut(s) 67, 474
SaqAI TTAA 3 cut(s) 300, 545, 1114
SatI GCNGC 6 cut(s) 591, 671, 735, 768, 957, 1041
Sau3AI GATC 5 cut(s) 454, 533, 921, 1027, 1032
Sau96I GGNCC 1 cut(s) 747
SchI GAGTC 1 cut(s) 97
ScrFI CCNGG 1 cut(s) 745
SduI GDGCHC 1 cut(s) 61
SfcI CTRYAG 2 cut(s) 183, 768
SfuI TTCGAA 1 cut(s) 820
SinI GGWCC 1 cut(s) 747
SmiMI CAYNNNNRTG 2 cut(s) 67, 474
Sse9I AATT 5 cut(s) 10, 88, 136, 229, 927
StyD4I CCNGG 1 cut(s) 743
StyI CCWWGG 3 cut(s) 353, 432, 483
TaaI ACNGT 3 cut(s) 377, 473, 528
TaiI ACGT 2 cut(s) 790, 865
TaqI TCGA 6 cut(s) 388, 414, 536, 820, 1073, 1100
TaqII GACCGA 2 cut(s) 764, 816
TasI AATT 5 cut(s) 10, 88, 136, 229, 927
TatI WGTACW 1 cut(s) 195
TfiI GAWTC 5 cut(s) 211, 396, 817, 844, 892
Tru1I TTAA 3 cut(s) 300, 545, 1114
Tru9I TTAA 3 cut(s) 300, 545, 1114
TscAI CASTG 2 cut(s) 382, 510
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 6 cut(s) 590, 670, 734, 767, 956, 1040
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 9 cut(s) 47, 180, 203, 272, 492, 547, 651, 654, 1109
TspGWI ACGGA 1 cut(s) 950
TspRI CASTG 2 cut(s) 382, 510
VpaK11BI GGWCC 1 cut(s) 747
XapI RAATTY 2 cut(s) 10, 88
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.