AT1G56520

negative regulation of Rho guanyl-nucleotide exchange factor activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001561
Physical Location & Seq
Reverse (-)
21174609 .. 21179077
4469 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G56520.4

Sequence Viewer

Length: 2694 bp
ATGGCTTCTTCTTCTTCCTCACCTCGCAACTGGAGATACAATGTCTTCACGAGCTTCCATGGACCAGACGTCCGTATTAAATTTCTCAGTCATTTACGTCAACAGTTTATTTACAATGGAATTACTATGTTCGATGACAACGGGATCGAAAGAAGCCAAATTATCGCTCCAGCTCTCAAAAAAGCCATTGGAGAATCGAGGATCGCGATCTTATTGCTCTCGAAGAACTATGCTTCTTCCAGTTGGTCTTTGGATGAGCTATTGGAGATTTTAAAGTGCAAAGAAGATATAGGGCAAATAGTGATGACTGTCTTCTACGAAGTTGATCCTTCTGATGTCCGCAACCAAACCGGAGATTTTGGGATTGCTTTTAAAGAAACTTGTGCTCATAAAACAGAGGAGGAGAGGCAAAAATGGACCCAAGCTTTGACCTATGTGGGCAACATTGCCGGAGAAGACTTTAAACACTGGCCCAATGAAGCTAAAATGATCGAGAAGATTGCAAGAGATGTTTCAGATATACTAAACGTCACACCGTGTAGGGATTTTGATGGCATGGTTGGACTAAACGATCATTTGAGGGAAATGGAGTCTTTGCTAGATTTAAAGAATGATGGAGTTAAGATTGTTGGAATCTCTGGTCCTGCAGGCATTGGTAAAAGTACCATTGCTACAGCTTTACATGGTCGACTCTCTAACATGTTTCAGCGTACTTGTTTTGTGGACAATCTTAGGGAAAGCTATAAGATTGGTCTTGATGAGTATCGTTTGAAGTTGCACTTACAACAGCAACTTCTTGCATATGTTTTAAATCAGGATAAAATTAGGGTGGGCCATTTAAGTGTGATGAAAGAAAGGCTTGACGACTTGAGGGTTCTTATTATTCTTGATGATGTGGAGCATCTATATCAACTAGAGGCTTTGGCTGATATCAGGTGGTTTGGTCCTGGAAGTAGGGTGATAGTGACCACTGAAAACAGAGAGATTTTGCTGCAACATGGTATCAAGGATATATACCATGTGGGTTTTCCATCAGAAGGAGAAGCTCTAATGATCTTTTGTCTATCTGCTTTTAGACAACCCTCTCCACCTTATGGTTTTTTGAAGCTTACATATGAAGTTGCAAGTATTTGTGGTAATCTTCCATTGGGTCTGCATGTTTTGGGGACGTTATTGTGGGGAAAAAGTCAGGCTGACTGGATTGAAGAACTACCAAGGTTGAAAGACTGTCTTGATGGAAGAATTGAGAGTGTATTGAAAGTTGGCTATGAGAGTTTATATGAGAAAGACCAAGCTCTTTTTCTCCTCATTGCAGTCTACTTCAATTATGATTATGTTGATTATGTGACATCCATGCTAGAAAATACTAACGTATTGGATGTTAGACTTGGGTTGAAAAAACTAGCTAATAGATGTCTTATACAAATAGATATAGACCATAATCGCAAAAGTAGAGTCGTAATGAACCGGTTGCTACAAGTAATGGCTCGAGAAGTTATTTCCAAACAAAAAATTTCCAAACGAAAGATTCTAGAAGATCCCCAGGATATTTGTTATGTTCTAGAAGAGGCAAAGGGTAAAGGATCAGCTTTAGGATTATCATTGGACGTAGCAGAGATCAAAGAATTAGTAATAAACAAAAAGGCTTTTAAAAAAATGTGCAATCTTCTCATCTTAAAAGTCTTTAATGGGACGGATCCCCGAGATAGTAAATTGCACGTACCAGAGGAGATGGAGCTTCCATCTAGCATAAGGTTACTACATTGGGAGGCATACCCGAGAAAATCTTTTAGATTTGGTCCAGAAAATCTCGTCACACTCAACATGGAGTACAGTGAGCTCGAGAAGCTATGGAAAGGAACTCAGCCACTTGCAAATCTCAAGGAGATGAACTTGTGTGGGTCATCTTGTTTGAAGGAACTCCCAGATCTTTCGAAAGCAGCAAATCTGGAGAGATTGGATGTGGCTGAGTGCAATGCTTTGGTAGAGATTCCATCCTCAGTTGCGAATCTTCACAAAATAGTTAACTTACACATGGAATCCTGTGAAAGTCTAGAAGTCATTCCAACTCTCATCAACTTGGCATCTCTTAAGATTATCAACATACATGATTGCCCACGGTTGAAAAGTTTTCCAGATGTTCCCACCAGCCTCGAGGAACTTGTGATAGAGAAAACAGGGGTACAAGAATTGCCTGCATCATTTAGGCATTGCACTGGTGTTACTACTCTTTATATATGTTCCAATAGAAATCTCAAGACCTTCTCAACACATCTCCCCATGGGTCTAAGGAAGCTAGACCTAAGCAATTGTGGTATTGAGTGGGTTACAGATAGCATCAAAGATCTTCATAATCTATATTACCTTAAACTATCAGGCTGCAAAAGACTTGTGTCTTTGCCAGAACTCCCTTGTTCGCTCGAGTGTCTATTTGCAGAGGATTGTACATCACTAGAAAGAGTAAGTGACTCTCTAAACATTCCAAATGCGCAGTTCAATTTCATCAAATGCTTCACATTGGATAGAGAAGCACGACGAGCGATTATTCAACAATCGTTTGTTCATGGGAATGTTATCTTACCAGCAAGAGAAGTACTTGAAGAAGTCGATTACCGAGCGAGAGGAAATTGCTTAACAATTCCTCCTTCTGCTTTCAACAGATTTAAGGTTTGCGTTGTGTTGTCAATTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0002064 GO:0002065 GO:0002066 GO:0002093 GO:0002682 GO:0002683 GO:0002684 GO:0002685 GO:0002686 GO:0002687 GO:0002688 GO:0002690 GO:0002691 GO:0002693 GO:0002694 GO:0002695 GO:0003008 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005886 GO:0007088 GO:0007162 GO:0007275 GO:0007346 GO:0007399 GO:0007423 GO:0007517 GO:0007519 GO:0007600 GO:0007605 GO:0008104 GO:0008150 GO:0008285 GO:0008544 GO:0009605 GO:0009612 GO:0009628 GO:0009653 GO:0009790 GO:0009887 GO:0009888 GO:0009913 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010564 GO:0010646 GO:0010648 GO:0010830 GO:0010831 GO:0014706 GO:0014902 GO:0014904 GO:0016020 GO:0016043 GO:0016324 GO:0022008 GO:0022407 GO:0022408 GO:0022603 GO:0022607 GO:0023051 GO:0023057 GO:0030030 GO:0030154 GO:0030155 GO:0030175 GO:0030182 GO:0030334 GO:0030335 GO:0030336 GO:0030855 GO:0031175 GO:0031252 GO:0031253 GO:0031256 GO:0031344 GO:0031346 GO:0032091 GO:0032101 GO:0032103 GO:0032420 GO:0032421 GO:0032501 GO:0032502 GO:0032589 GO:0032878 GO:0032879 GO:0032880 GO:0032886 GO:0032944 GO:0032945 GO:0032989 GO:0033036 GO:0033043 GO:0034097 GO:0035023 GO:0035024 GO:0035315 GO:0040012 GO:0040013 GO:0040017 GO:0042127 GO:0042129 GO:0042130 GO:0042221 GO:0042471 GO:0042472 GO:0042490 GO:0042491 GO:0042692 GO:0042802 GO:0042995 GO:0043005 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043393 GO:0043583 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044092 GO:0044093 GO:0044422 GO:0044424 GO:0044425 GO:0044459 GO:0044463 GO:0044464 GO:0045177 GO:0045184 GO:0045595 GO:0045597 GO:0045661 GO:0045663 GO:0045787 GO:0046578 GO:0046580 GO:0048468 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048562 GO:0048568 GO:0048583 GO:0048584 GO:0048585 GO:0048598 GO:0048666 GO:0048667 GO:0048699 GO:0048731 GO:0048741 GO:0048747 GO:0048839 GO:0048856 GO:0048869 GO:0050670 GO:0050672 GO:0050789 GO:0050793 GO:0050794 GO:0050863 GO:0050865 GO:0050866 GO:0050868 GO:0050877 GO:0050896 GO:0050920 GO:0050921 GO:0050954 GO:0051056 GO:0051058 GO:0051094 GO:0051098 GO:0051099 GO:0051100 GO:0051128 GO:0051130 GO:0051146 GO:0051147 GO:0051149 GO:0051153 GO:0051155 GO:0051179 GO:0051234 GO:0051249 GO:0051250 GO:0051259 GO:0051260 GO:0051270 GO:0051271 GO:0051272 GO:0051489 GO:0051491 GO:0051493 GO:0051716 GO:0051726 GO:0051783 GO:0055001 GO:0055002 GO:0060088 GO:0060113 GO:0060117 GO:0060119 GO:0060122 GO:0060142 GO:0060143 GO:0060171 GO:0060236 GO:0060341 GO:0060429 GO:0060491 GO:0060537 GO:0060538 GO:0060563 GO:0061061 GO:0065003 GO:0065007 GO:0065009 GO:0070507 GO:0070663 GO:0070664 GO:0070887 GO:0071156 GO:0071158 GO:0071214 GO:0071260 GO:0071310 GO:0071345 GO:0071496 GO:0071622 GO:0071624 GO:0071840 GO:0071889 GO:0071944 GO:0090022 GO:0090023 GO:0090068 GO:0090169 GO:0090224 GO:0090596 GO:0097458 GO:0098590 GO:0098858 GO:0098862 GO:0104004 GO:0120025 GO:0120032 GO:0120034 GO:0120035 GO:0120036 GO:0120038 GO:1901673 GO:1901739 GO:1901741 GO:1902115 GO:1902531 GO:1902532 GO:1902622 GO:1902624 GO:1903037 GO:1903038 GO:1903827 GO:1903828 GO:1903903 GO:1903904 GO:1904424 GO:1904475 GO:1904476 GO:1905097 GO:1905098 GO:1905871 GO:1905872 GO:1990868 GO:1990869 GO:2000114 GO:2000145 GO:2000146 GO:2000147 GO:2000389 GO:2000391 GO:2000401 GO:2000402 GO:2000404 GO:2000405 GO:2001106 GO:2001107
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC PL48 TIR

Protein Analysis

897

Amino Acids

101.81

Weight (kDa)

6.26

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 13 - 187 1.3e-53 TIR domain
TIR_2 PF13676 15 - 110 2.3e-07 TIR domain
NB-ARC PF00931 198 - 361 5.9e-11 NB-ARC domain
WHD_ROQ1 PF23282 428 - 503 2.7e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 603 - 622 6.5e-08 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 72
AbsI CCTCGAGG 1 cut(s) 2153
Acc16I TGCGCA 1 cut(s) 2490
AccB7I CCANNNNNTGG 1 cut(s) 1094
AccI GTMKAC 2 cut(s) 688, 1317
AccII CGCG 1 cut(s) 206
AciI CCGC 1 cut(s) 340
AclWI GGATC 7 cut(s) 152, 209, 320, 1532, 1591, 1691, 1704
AcsI RAATTY 2 cut(s) 80, 1512
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 537
AfaI GTAC 7 cut(s) 664, 712, 1722, 1832, 2184, 2446, 2595
AfiI CCNNNNNNNGG 2 cut(s) 1037, 1094
AflII CTTAAG 1 cut(s) 2090
AflIII ACRYGT 1 cut(s) 699
AgeI ACCGGT 1 cut(s) 1467
AhdI GACNNNNNGTC 1 cut(s) 68
AjnI CCWGG 2 cut(s) 946, 1542
AjuI GAANNNNNNNTTGG 2 cut(s) 1284, 1316
Alw21I GWGCWC 2 cut(s) 388, 1842
AlwI GGATC 7 cut(s) 152, 209, 320, 1532, 1591, 1691, 1704
Ama87I CYCGRG 6 cut(s) 1488, 1701, 1777, 1841, 2153, 2420
AoxI GGCC 2 cut(s) 470, 832
ApeKI GCWGC 3 cut(s) 991, 1940, 2379
ApoI RAATTY 2 cut(s) 80, 1512
ArsI GACNNNNNNTTYG 4 cut(s) 409, 441, 1440, 1472
AsiGI ACCGGT 1 cut(s) 1467
Asp700I GAANNNNTTC 1 cut(s) 2061
AspLEI GCGC 1 cut(s) 2491
AspS9I GGNCC 7 cut(s) 62, 417, 471, 641, 832, 944, 1799
AsuHPI GGTGA 2 cut(s) 12, 970
AsuII TTCGAA 1 cut(s) 1934
AvaI CYCGRG 6 cut(s) 1488, 1701, 1777, 1841, 2153, 2420
AvaII GGWCC 5 cut(s) 62, 417, 641, 944, 1799
BamHI GGATCC 1 cut(s) 1696
BanII GRGCYC 1 cut(s) 1842
BauI CACGAG 1 cut(s) 49
BbsI GAAGAC 3 cut(s) 37, 304, 462
Bbv12I GWGCWC 2 cut(s) 388, 1842
BbvI GCAGC 3 cut(s) 978, 1952, 2366
BccI CCATC 7 cut(s) 545, 608, 1039, 1229, 1726, 1750, 2002
BcgI CGANNNNNNTGC 4 cut(s) 196, 230, 482, 516
BciT130I CCWGG 2 cut(s) 948, 1544
BfmI CTRYAG 2 cut(s) 645, 672
BfrI CTTAAG 1 cut(s) 2090
BglII AGATCT 2 cut(s) 1927, 2344
BisI GCNGC 3 cut(s) 992, 1941, 2380
BlsI GCNGC 3 cut(s) 993, 1942, 2381
BmcAI AGTACT 1 cut(s) 2595
Bme1390I CCNGG 2 cut(s) 948, 1544
Bme18I GGWCC 5 cut(s) 62, 417, 641, 944, 1799
BmeRI GACNNNNNGTC 1 cut(s) 68
BmeT110I CYCGRG 6 cut(s) 1488, 1701, 1777, 1841, 2153, 2420
BmgT120I GGNCC 7 cut(s) 62, 417, 471, 641, 832, 944, 1799
BmiI GGNNCC 2 cut(s) 419, 1698
BmrFI CCNGG 2 cut(s) 948, 1544
BmsI GCATC 4 cut(s) 910, 2093, 2207, 2346
BoxI GACNNNNGTC 1 cut(s) 2392
BpiI GAAGAC 3 cut(s) 37, 304, 462
BpmI CTGGAG 3 cut(s) 52, 153, 1970
Bpu10I CCTNAGC 1 cut(s) 2303
Bpu14I TTCGAA 1 cut(s) 1934
BpuEI CTTGAG 3 cut(s) 889, 1865, 2240
BsaAI YACGTR 1 cut(s) 1720
BsaBI GATNNNNATC 2 cut(s) 206, 762
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 5 cut(s) 58, 1214, 1542, 2117, 2280
BsaWI WCCGGW 2 cut(s) 350, 1467
BsaXI ACNNNNNCTCC 2 cut(s) 2626, 2656
Bsc4I CCNNNNNNNGG 2 cut(s) 1037, 1094
Bse118I RCCGGY 1 cut(s) 1467
Bse1I ACTGG 5 cut(s) 35, 240, 473, 1202, 2221
Bse3DI GCAATG 5 cut(s) 444, 666, 1308, 1981, 2209
Bse8I GATNNNNATC 2 cut(s) 206, 762
BseBI CCWGG 2 cut(s) 948, 1544
BseDI CCNNGG 5 cut(s) 58, 1214, 1542, 2117, 2280
BseGI GGATG 5 cut(s) 259, 1349, 1384, 1966, 1994
BseJI GATNNNNATC 2 cut(s) 206, 762
BseLI CCNNNNNNNGG 2 cut(s) 1037, 1094
BseMI GCAATG 5 cut(s) 444, 666, 1308, 1981, 2209
BseMII CTCAG 4 cut(s) 100, 1877, 1959, 2013
BseNI ACTGG 5 cut(s) 35, 240, 473, 1202, 2221
BseRI GAGGAG 4 cut(s) 413, 416, 1295, 1742
BseXI GCAGC 3 cut(s) 978, 1952, 2366
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 472, 834
BshTI ACCGGT 1 cut(s) 1467
BsiHKAI GWGCWC 2 cut(s) 388, 1842
BsiHKCI CYCGRG 6 cut(s) 1488, 1701, 1777, 1841, 2153, 2420
BsiSI CCGG 3 cut(s) 351, 450, 1468
BslFI GGGAC 2 cut(s) 1180, 1705
BslI CCNNNNNNNGG 2 cut(s) 1037, 1094
BsmFI GGGAC 2 cut(s) 1180, 1705
BsnI GGCC 2 cut(s) 472, 834
BsoBI CYCGRG 6 cut(s) 1488, 1701, 1777, 1841, 2153, 2420
Bsp119I TTCGAA 1 cut(s) 1934
Bsp1286I GDGCHC 2 cut(s) 388, 1842
Bsp1407I TGTACA 1 cut(s) 2444
Bsp19I CCATGG 2 cut(s) 58, 2280
Bsp68I TCGCGA 1 cut(s) 206
BspACI CCGC 1 cut(s) 340
BspANI GGCC 2 cut(s) 472, 834
BspCNI CTCAG 4 cut(s) 99, 1876, 1960, 2012
BspFNI CGCG 1 cut(s) 206
BspLI GGNNCC 2 cut(s) 419, 1698
BspMAI CTGCAG 1 cut(s) 649
BspPI GGATC 7 cut(s) 152, 209, 320, 1532, 1591, 1691, 1704
BspT104I TTCGAA 1 cut(s) 1934
BspTI CTTAAG 1 cut(s) 2090
BsrDI GCAATG 5 cut(s) 444, 666, 1308, 1981, 2209
BsrFI RCCGGY 1 cut(s) 1467
BsrGI TGTACA 1 cut(s) 2444
BsrI ACTGG 5 cut(s) 35, 240, 473, 1202, 2221
BssAI RCCGGY 1 cut(s) 1467
BssECI CCNNGG 5 cut(s) 58, 1214, 1542, 2117, 2280
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 49
BssT1I CCWWGG 3 cut(s) 58, 1214, 2280
Bst2BI CACGAG 1 cut(s) 49
Bst2UI CCWGG 2 cut(s) 948, 1544
Bst4CI ACNGT 6 cut(s) 105, 310, 537, 1229, 1835, 2121
Bst6I CTCTTC 1 cut(s) 1560
BstACI GRCGYC 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 2090
BstAUI TGTACA 1 cut(s) 2444
BstBAI YACGTR 1 cut(s) 1720
BstBI TTCGAA 1 cut(s) 1934
BstC8I GCNNGC 2 cut(s) 649, 2196
BstDEI CTNAG 7 cut(s) 86, 731, 1863, 1968, 1999, 2288, 2303
BstDSI CCRYGG 3 cut(s) 58, 2117, 2280
BstF5I GGATG 5 cut(s) 259, 1349, 1384, 1966, 1994
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 1 cut(s) 2491
BstMWI GCNNNNNNNGC 2 cut(s) 1846, 2537
BstNI CCWGG 2 cut(s) 948, 1544
BstNSI RCATGY 2 cut(s) 703, 1160
BstPAI GACNNNNGTC 1 cut(s) 2392
BstSCI CCNGG 2 cut(s) 946, 1542
BstSFI CTRYAG 2 cut(s) 645, 672
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 3 cut(s) 978, 1952, 2366
BstV2I GAAGAC 3 cut(s) 37, 304, 462
BstX2I RGATCY 4 cut(s) 1537, 1696, 1927, 2344
BstYI RGATCY 4 cut(s) 1537, 1696, 1927, 2344
BsuRI GGCC 2 cut(s) 472, 834
BtgI CCRYGG 3 cut(s) 58, 2117, 2280
BtsCI GGATG 5 cut(s) 259, 1349, 1384, 1966, 1994
BtsIMutI CAGTG 4 cut(s) 466, 969, 1840, 2214
BtuMI TCGCGA 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 649, 2196
CfoI GCGC 1 cut(s) 2491
Cfr10I RCCGGY 1 cut(s) 1467
Cfr13I GGNCC 7 cut(s) 62, 417, 471, 641, 832, 944, 1799
Csp6I GTAC 7 cut(s) 663, 711, 1721, 1831, 2183, 2445, 2594
CspAI ACCGGT 1 cut(s) 1467
CviQI GTAC 7 cut(s) 663, 711, 1721, 1831, 2183, 2445, 2594
DdeI CTNAG 7 cut(s) 86, 731, 1863, 1968, 1999, 2288, 2303
DraI TTTAAA 6 cut(s) 273, 373, 463, 606, 810, 1651
DraIII CACNNNGTG 1 cut(s) 537
DriI GACNNNNNGTC 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 1560
Eam1105I GACNNNNNGTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 1560
Ecl136II GAGCTC 1 cut(s) 1840
Eco130I CCWWGG 3 cut(s) 58, 1214, 2280
Eco24I GRGCYC 1 cut(s) 1842
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 5 cut(s) 62, 417, 641, 944, 1799
Eco53kI GAGCTC 1 cut(s) 1840
Eco88I CYCGRG 6 cut(s) 1488, 1701, 1777, 1841, 2153, 2420
EcoICRI GAGCTC 1 cut(s) 1840
EcoRII CCWGG 2 cut(s) 946, 1542
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 3 cut(s) 58, 1214, 2280
EcoT38I GRGCYC 1 cut(s) 1842
ErhI CCWWGG 3 cut(s) 58, 1214, 2280
FalI AAGNNNNNCTT 6 cut(s) 764, 796, 843, 875, 1215, 1247
FaqI GGGAC 2 cut(s) 1180, 1705
FauNDI CATATG 2 cut(s) 802, 1114
FblI GTMKAC 2 cut(s) 688, 1317
Fnu4HI GCNGC 3 cut(s) 992, 1941, 2380
FokI GGATG 5 cut(s) 266, 1336, 1391, 1973, 1981
FriOI GRGCYC 1 cut(s) 1842
Fsp4HI GCNGC 3 cut(s) 992, 1941, 2380
FspI TGCGCA 1 cut(s) 2490
GlaI GCGC 1 cut(s) 2490
GluI GCNGC 3 cut(s) 992, 1941, 2380
GsuI CTGGAG 3 cut(s) 52, 153, 1970
HaeIII GGCC 2 cut(s) 472, 834
HapII CCGG 3 cut(s) 351, 450, 1468
HhaI GCGC 1 cut(s) 2491
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 1 cut(s) 2489
HinP1I GCGC 1 cut(s) 2489
HincII GTYRAC 3 cut(s) 101, 689, 2026
HindII GTYRAC 3 cut(s) 101, 689, 2026
HindIII AAGCTT 2 cut(s) 423, 1106
HpaI GTTAAC 1 cut(s) 2026
HpaII CCGG 3 cut(s) 351, 450, 1468
HphI GGTGA 2 cut(s) 12, 970
Hpy166II GTNNAC 5 cut(s) 101, 689, 724, 1318, 2026
Hpy188I TCNGA 3 cut(s) 334, 517, 1036
Hpy8I GTNNAC 5 cut(s) 101, 689, 724, 1318, 2026
Hpy99I CGWCG 1 cut(s) 2538
HpyAV CCTTC 5 cut(s) 339, 1031, 1909, 2272, 2655
HpyCH4III ACNGT 6 cut(s) 105, 310, 537, 1229, 1835, 2121
HpyCH4IV ACGT 7 cut(s) 69, 97, 528, 1169, 1371, 1608, 1719
HpyF10VI GCNNNNNNNGC 2 cut(s) 1846, 2537
HpyF3I CTNAG 7 cut(s) 86, 731, 1863, 1968, 1999, 2288, 2303
HpySE526I ACGT 7 cut(s) 69, 97, 528, 1169, 1371, 1608, 1719
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 1 cut(s) 2489
KspAI GTTAAC 1 cut(s) 2026
LmnI GCTCC 3 cut(s) 172, 898, 1735
Lsp1109I GCAGC 3 cut(s) 978, 1952, 2366
LweI GCATC 4 cut(s) 910, 2093, 2207, 2346
MaeII ACGT 7 cut(s) 69, 97, 528, 1169, 1371, 1608, 1719
MaeIII GTNAC 8 cut(s) 529, 964, 1345, 1755, 1813, 2221, 2326, 2465
MfeI CAATTG 1 cut(s) 2308
MflI RGATCY 4 cut(s) 1537, 1696, 1927, 2344
MhlI GDGCHC 2 cut(s) 388, 1842
MlyI GAGTC 4 cut(s) 599, 684, 1464, 2462
MmeI TCCRAC 3 cut(s) 541, 610, 2090
MroXI GAANNNNTTC 1 cut(s) 2061
MslI CAYNNNNRTG 2 cut(s) 840, 2568
MspCI CTTAAG 1 cut(s) 2090
MspI CCGG 3 cut(s) 351, 450, 1468
MspR9I CCNGG 2 cut(s) 948, 1544
MunI CAATTG 1 cut(s) 2308
MvaI CCWGG 2 cut(s) 948, 1544
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 2 cut(s) 1846, 2537
NcoI CCATGG 2 cut(s) 58, 2280
NdeI CATATG 2 cut(s) 802, 1114
NlaIV GGNNCC 2 cut(s) 419, 1698
NmuCI GTSAC 5 cut(s) 529, 964, 1345, 1813, 2465
NruI TCGCGA 1 cut(s) 206
NsbI TGCGCA 1 cut(s) 2490
NspI RCATGY 2 cut(s) 703, 1160
NspV TTCGAA 1 cut(s) 1934
PaeR7I CTCGAG 4 cut(s) 1488, 1841, 2153, 2420
PciI ACATGT 1 cut(s) 699
PcsI WCGNNNNNNNCGW 1 cut(s) 203
PdmI GAANNNNTTC 1 cut(s) 2061
PfeI GAWTC 6 cut(s) 194, 633, 1528, 1990, 2008, 2039
PflMI CCANNNNNTGG 1 cut(s) 1094
PfoI TCCNGGA 1 cut(s) 946
PinAI ACCGGT 1 cut(s) 1467
PkrI GCNGC 3 cut(s) 993, 1942, 2381
PleI GAGTC 4 cut(s) 598, 684, 1463, 2462
PpsI GAGTC 4 cut(s) 598, 684, 1463, 2462
Ppu21I YACGTR 1 cut(s) 1720
PscI ACATGT 1 cut(s) 699
PshAI GACNNNNGTC 1 cut(s) 2392
Psp124BI GAGCTC 1 cut(s) 1842
Psp6I CCWGG 2 cut(s) 946, 1542
PspGI CCWGG 2 cut(s) 946, 1542
PspN4I GGNNCC 2 cut(s) 419, 1698
PspPI GGNCC 7 cut(s) 62, 417, 471, 641, 832, 944, 1799
PspXI VCTCGAGB 2 cut(s) 2153, 2420
PstI CTGCAG 1 cut(s) 649
PsuI RGATCY 4 cut(s) 1537, 1696, 1927, 2344
RruI TCGCGA 1 cut(s) 206
RsaI GTAC 7 cut(s) 664, 712, 1722, 1832, 2184, 2446, 2595
RsaNI GTAC 7 cut(s) 663, 711, 1721, 1831, 2183, 2445, 2594
RseI CAYNNNNRTG 2 cut(s) 840, 2568
SacI GAGCTC 1 cut(s) 1842
SalI GTCGAC 1 cut(s) 687
SatI GCNGC 3 cut(s) 992, 1941, 2380
Sau96I GGNCC 7 cut(s) 62, 417, 471, 641, 832, 944, 1799
SbfI CCTGCAGG 1 cut(s) 649
ScaI AGTACT 1 cut(s) 2595
SchI GAGTC 4 cut(s) 599, 684, 1464, 2462
ScrFI CCNGG 2 cut(s) 948, 1544
SdaI CCTGCAGG 1 cut(s) 649
SduI GDGCHC 2 cut(s) 388, 1842
SfaNI GCATC 4 cut(s) 910, 2093, 2207, 2346
SfcI CTRYAG 2 cut(s) 645, 672
Sfr274I CTCGAG 4 cut(s) 1488, 1841, 2153, 2420
SfuI TTCGAA 1 cut(s) 1934
SinI GGWCC 5 cut(s) 62, 417, 641, 944, 1799
SlaI CTCGAG 4 cut(s) 1488, 1841, 2153, 2420
SmiMI CAYNNNNRTG 2 cut(s) 840, 2568
SmlI CTYRAG 8 cut(s) 868, 1488, 1841, 1880, 2090, 2153, 2255, 2420
SmoI CTYRAG 8 cut(s) 868, 1488, 1841, 1880, 2090, 2153, 2255, 2420
Sse8387I CCTGCAGG 1 cut(s) 649
SsiI CCGC 1 cut(s) 340
SstI GAGCTC 1 cut(s) 1842
StyD4I CCNGG 2 cut(s) 946, 1542
StyI CCWWGG 3 cut(s) 58, 1214, 2280
TaaI ACNGT 6 cut(s) 105, 310, 537, 1229, 1835, 2121
TaiI ACGT 7 cut(s) 72, 100, 531, 1172, 1374, 1611, 1722
TatI WGTACW 3 cut(s) 1830, 2444, 2593
TfiI GAWTC 6 cut(s) 194, 633, 1528, 1990, 2008, 2039
TscAI CASTG 4 cut(s) 473, 976, 1840, 2221
TseFI GTSAC 5 cut(s) 529, 964, 1345, 1813, 2465
TseI GCWGC 3 cut(s) 991, 1940, 2379
Tsp45I GTSAC 5 cut(s) 529, 964, 1345, 1813, 2465
TspDTI ATGAA 8 cut(s) 492, 863, 1131, 1478, 1904, 2339, 2491, 2552
TspGWI ACGGA 2 cut(s) 62, 1709
TspRI CASTG 4 cut(s) 473, 976, 1840, 2221
Van91I CCANNNNNTGG 1 cut(s) 1094
Vha464I CTTAAG 1 cut(s) 2090
VpaK11BI GGWCC 5 cut(s) 62, 417, 641, 944, 1799
XapI RAATTY 2 cut(s) 80, 1512
XbaI TCTAGA 3 cut(s) 1531, 1561, 2053
XceI RCATGY 2 cut(s) 703, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 247
XhoI CTCGAG 4 cut(s) 1488, 1841, 2153, 2420
XmiI GTMKAC 2 cut(s) 688, 1317
XmnI GAANNNNTTC 1 cut(s) 2061
ZraI GACGTC 1 cut(s) 70
ZrmI AGTACT 1 cut(s) 2595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.