negative regulation of Rho guanyl-nucleotide exchange factor activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 75 |
| Acc65I | GGTACC | 1 cut(s) | 1568 |
| AccB1I | GGYRCC | 1 cut(s) | 1568 |
| AccI | GTMKAC | 4 cut(s) | 691, 1159, 1480, 2281 |
| AccII | CGCG | 1 cut(s) | 2660 |
| AciI | CCGC | 3 cut(s) | 343, 1799, 2936 |
| AclWI | GGATC | 6 cut(s) | 73, 212, 323, 374, 1526, 2043 |
| AcoI | YGGCCR | 1 cut(s) | 1850 |
| AcsI | RAATTY | 7 cut(s) | 588, 1790, 1888, 1942, 2494, 2620, 2631 |
| AcuI | CTGAAG | 1 cut(s) | 2874 |
| AcyI | GRCGYC | 2 cut(s) | 72, 2081 |
| AfaI | GTAC | 7 cut(s) | 31, 79, 667, 1260, 1570, 1844, 2099 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1978 |
| AflIII | ACRYGT | 3 cut(s) | 383, 1162, 2719 |
| AhlI | ACTAGT | 1 cut(s) | 1471 |
| AjiI | CACGTC | 2 cut(s) | 1097, 2720 |
| AjnI | CCWGG | 3 cut(s) | 952, 1852, 2575 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1874, 1906 |
| Alw21I | GWGCWC | 1 cut(s) | 1091 |
| Alw26I | GTCTC | 3 cut(s) | 2084, 2088, 2556 |
| AlwI | GGATC | 6 cut(s) | 73, 212, 323, 374, 1526, 2043 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1867 |
| Ama87I | CYCGRG | 2 cut(s) | 1838, 2275 |
| AoxI | GGCC | 1 cut(s) | 1850 |
| ApeKI | GCWGC | 5 cut(s) | 1864, 2376, 2692, 2862, 2973 |
| ApoI | RAATTY | 7 cut(s) | 588, 1790, 1888, 1942, 2494, 2620, 2631 |
| AseI | ATTAAT | 1 cut(s) | 1611 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 795, 2049 |
| Asp718I | GGTACC | 1 cut(s) | 1568 |
| AspA2I | CCTAGG | 1 cut(s) | 2687 |
| AspLEI | GCGC | 3 cut(s) | 178, 783, 2897 |
| AspS9I | GGNCC | 2 cut(s) | 644, 950 |
| AsuC2I | CCSGG | 2 cut(s) | 244, 2809 |
| AsuHPI | GGTGA | 3 cut(s) | 1355, 2491, 2531 |
| AsuII | TTCGAA | 1 cut(s) | 2815 |
| AvaI | CYCGRG | 2 cut(s) | 1838, 2275 |
| AvaII | GGWCC | 2 cut(s) | 644, 950 |
| AvrII | CCTAGG | 1 cut(s) | 2687 |
| AxyI | CCTNAGG | 2 cut(s) | 734, 1742 |
| BalI | TGGCCA | 1 cut(s) | 1852 |
| BanI | GGYRCC | 1 cut(s) | 1568 |
| BanII | GRGCYC | 1 cut(s) | 1091 |
| BauI | CACGAG | 2 cut(s) | 52, 1391 |
| BbsI | GAAGAC | 4 cut(s) | 40, 307, 1772, 2042 |
| Bbv12I | GWGCWC | 1 cut(s) | 1091 |
| BbvI | GCAGC | 5 cut(s) | 1851, 2363, 2704, 2849, 2960 |
| BceAI | ACGGC | 1 cut(s) | 2445 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2195, 2229 |
| BciT130I | CCWGG | 3 cut(s) | 954, 1854, 2577 |
| BclI | TGATCA | 1 cut(s) | 139 |
| BcnI | CCSGG | 2 cut(s) | 244, 2809 |
| BcoDI | GTCTC | 3 cut(s) | 2084, 2088, 2556 |
| BcuI | ACTAGT | 1 cut(s) | 1471 |
| BfmI | CTRYAG | 2 cut(s) | 648, 2377 |
| BfoI | RGCGCY | 1 cut(s) | 2898 |
| BglII | AGATCT | 4 cut(s) | 1059, 1924, 1957, 2293 |
| BisI | GCNGC | 5 cut(s) | 1865, 2377, 2693, 2863, 2974 |
| BlnI | CCTAGG | 1 cut(s) | 2687 |
| BlsI | GCNGC | 5 cut(s) | 1866, 2378, 2694, 2864, 2975 |
| BmcAI | AGTACT | 1 cut(s) | 1260 |
| Bme1390I | CCNGG | 5 cut(s) | 244, 954, 1854, 2577, 2809 |
| Bme18I | GGWCC | 2 cut(s) | 644, 950 |
| BmeT110I | CYCGRG | 2 cut(s) | 1838, 2275 |
| BmgBI | CACGTC | 2 cut(s) | 1097, 2720 |
| BmgT120I | GGNCC | 2 cut(s) | 644, 950 |
| BmiI | GGNNCC | 2 cut(s) | 422, 1570 |
| BmrFI | CCNGG | 5 cut(s) | 244, 954, 1854, 2577, 2809 |
| BmrI | ACTGGG | 1 cut(s) | 481 |
| BmsI | GCATC | 7 cut(s) | 1519, 1750, 2099, 2204, 2420, 2660, 2857 |
| BmuI | ACTGGG | 1 cut(s) | 481 |
| BpiI | GAAGAC | 4 cut(s) | 40, 307, 1772, 2042 |
| BpmI | CTGGAG | 2 cut(s) | 375, 2471 |
| Bpu14I | TTCGAA | 1 cut(s) | 2815 |
| BpuEI | CTTGAG | 6 cut(s) | 168, 172, 1518, 1862, 2108, 2237 |
| BpuMI | CCSGG | 2 cut(s) | 244, 2809 |
| BsaAI | YACGTR | 1 cut(s) | 1163 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1344 |
| BsaHI | GRCGYC | 2 cut(s) | 72, 2081 |
| BsaJI | CCNNGG | 4 cut(s) | 61, 1505, 2575, 2687 |
| BsaWI | WCCGGW | 2 cut(s) | 1571, 2959 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 28, 58 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1978 |
| Bse118I | RCCGGY | 1 cut(s) | 1833 |
| Bse1I | ACTGG | 4 cut(s) | 358, 476, 2110, 2454 |
| Bse21I | CCTNAGG | 2 cut(s) | 734, 1742 |
| Bse3DI | GCAATG | 3 cut(s) | 1314, 2206, 2393 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1344 |
| BseBI | CCWGG | 3 cut(s) | 954, 1854, 2577 |
| BseDI | CCNNGG | 4 cut(s) | 61, 1505, 2575, 2687 |
| BseGI | GGATG | 6 cut(s) | 262, 292, 745, 1444, 1765, 2114 |
| BseJI | GATNNNNATC | 1 cut(s) | 1344 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1978 |
| BseMI | GCAATG | 3 cut(s) | 1314, 2206, 2393 |
| BseMII | CTCAG | 4 cut(s) | 725, 1874, 1952, 2311 |
| BseNI | ACTGG | 4 cut(s) | 358, 476, 2110, 2454 |
| BseRI | GAGGAG | 2 cut(s) | 416, 419 |
| BseXI | GCAGC | 5 cut(s) | 1851, 2363, 2704, 2849, 2960 |
| Bsh1236I | CGCG | 1 cut(s) | 2660 |
| BshFI | GGCC | 1 cut(s) | 1852 |
| BshNI | GGYRCC | 1 cut(s) | 1568 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1091 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1838, 2275 |
| BsiSI | CCGG | 5 cut(s) | 243, 1572, 1834, 2808, 2960 |
| BslFI | GGGAC | 2 cut(s) | 488, 2769 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1978 |
| BsmAI | GTCTC | 3 cut(s) | 2084, 2088, 2556 |
| BsmBI | CGTCTC | 1 cut(s) | 2088 |
| BsmFI | GGGAC | 2 cut(s) | 488, 2769 |
| BsmI | GAATGC | 1 cut(s) | 1458 |
| BsnI | GGCC | 1 cut(s) | 1852 |
| BsoBI | CYCGRG | 2 cut(s) | 1838, 2275 |
| Bsp119I | TTCGAA | 1 cut(s) | 2815 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1091 |
| Bsp1407I | TGTACA | 1 cut(s) | 2097 |
| BspACI | CCGC | 3 cut(s) | 343, 1799, 2936 |
| BspANI | GGCC | 1 cut(s) | 1852 |
| BspCNI | CTCAG | 4 cut(s) | 726, 1873, 1953, 2310 |
| BspFNI | CGCG | 1 cut(s) | 2660 |
| BspLI | GGNNCC | 2 cut(s) | 422, 1570 |
| BspMAI | CTGCAG | 2 cut(s) | 652, 2381 |
| BspPI | GGATC | 6 cut(s) | 73, 212, 323, 374, 1526, 2043 |
| BspQI | GCTCTTC | 2 cut(s) | 1096, 1494 |
| BspT104I | TTCGAA | 1 cut(s) | 2815 |
| BspT107I | GGYRCC | 1 cut(s) | 1568 |
| BsrDI | GCAATG | 3 cut(s) | 1314, 2206, 2393 |
| BsrFI | RCCGGY | 1 cut(s) | 1833 |
| BsrGI | TGTACA | 1 cut(s) | 2097 |
| BsrI | ACTGG | 4 cut(s) | 358, 476, 2110, 2454 |
| BssAI | RCCGGY | 1 cut(s) | 1833 |
| BssECI | CCNNGG | 4 cut(s) | 61, 1505, 2575, 2687 |
| BssNI | GRCGYC | 2 cut(s) | 72, 2081 |
| BssSI | CACGAG | 2 cut(s) | 52, 1391 |
| BssT1I | CCWWGG | 2 cut(s) | 1505, 2687 |
| Bst2BI | CACGAG | 2 cut(s) | 52, 1391 |
| Bst2UI | CCWGG | 3 cut(s) | 954, 1854, 2577 |
| Bst4CI | ACNGT | 6 cut(s) | 108, 313, 2118, 2550, 2733, 2760 |
| Bst6I | CTCTTC | 3 cut(s) | 19, 1096, 1494 |
| BstACI | GRCGYC | 2 cut(s) | 72, 2081 |
| BstAUI | TGTACA | 1 cut(s) | 2097 |
| BstBAI | YACGTR | 1 cut(s) | 1163 |
| BstBI | TTCGAA | 1 cut(s) | 2815 |
| BstC8I | GCNNGC | 8 cut(s) | 454, 652, 1365, 1835, 2193, 2565, 2569, 2671 |
| BstDSI | CCRYGG | 1 cut(s) | 61 |
| BstF5I | GGATG | 6 cut(s) | 262, 292, 745, 1444, 1765, 2114 |
| BstFNI | CGCG | 1 cut(s) | 2660 |
| BstH2I | RGCGCY | 1 cut(s) | 2898 |
| BstHHI | GCGC | 3 cut(s) | 178, 783, 2897 |
| BstMAI | GTCTC | 3 cut(s) | 2084, 2088, 2556 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 997, 1364, 2657, 2666, 2692, 2982 |
| BstNI | CCWGG | 3 cut(s) | 954, 1854, 2577 |
| BstNSI | RCATGY | 2 cut(s) | 387, 2571 |
| BstSCI | CCNGG | 5 cut(s) | 242, 952, 1852, 2575, 2807 |
| BstSFI | CTRYAG | 2 cut(s) | 648, 2377 |
| BstUI | CGCG | 1 cut(s) | 2660 |
| BstV1I | GCAGC | 5 cut(s) | 1851, 2363, 2704, 2849, 2960 |
| BstV2I | GAAGAC | 4 cut(s) | 40, 307, 1772, 2042 |
| BstX2I | RGATCY | 8 cut(s) | 204, 328, 1059, 1518, 1924, 1957, 2035, 2293 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 752 |
| BstYI | RGATCY | 8 cut(s) | 204, 328, 1059, 1518, 1924, 1957, 2035, 2293 |
| Bsu36I | CCTNAGG | 2 cut(s) | 734, 1742 |
| BsuRI | GGCC | 1 cut(s) | 1852 |
| BtgI | CCRYGG | 1 cut(s) | 61 |
| BtgZI | GCGATG | 1 cut(s) | 2880 |
| BtrI | CACGTC | 2 cut(s) | 1097, 2720 |
| BtsCI | GGATG | 6 cut(s) | 262, 292, 745, 1444, 1765, 2114 |
| BtsI | GCAGTG | 1 cut(s) | 2992 |
| BtsIMutI | CAGTG | 2 cut(s) | 2447, 2992 |
| Cac8I | GCNNGC | 8 cut(s) | 454, 652, 1365, 1835, 2193, 2565, 2569, 2671 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1867 |
| CfoI | GCGC | 3 cut(s) | 178, 783, 2897 |
| Cfr10I | RCCGGY | 1 cut(s) | 1833 |
| Cfr13I | GGNCC | 2 cut(s) | 644, 950 |
| CseI | GACGC | 2 cut(s) | 2070, 2662 |
| Csp6I | GTAC | 7 cut(s) | 30, 78, 666, 1259, 1569, 1843, 2098 |
| CviQI | GTAC | 7 cut(s) | 30, 78, 666, 1259, 1569, 1843, 2098 |
| DraI | TTTAAA | 2 cut(s) | 1108, 2472 |
| EaeI | YGGCCR | 1 cut(s) | 1850 |
| Eam1104I | CTCTTC | 3 cut(s) | 19, 1096, 1494 |
| EarI | CTCTTC | 3 cut(s) | 19, 1096, 1494 |
| EciI | GGCGGA | 1 cut(s) | 1814 |
| Ecl136II | GAGCTC | 1 cut(s) | 1089 |
| Eco130I | CCWWGG | 2 cut(s) | 1505, 2687 |
| Eco24I | GRGCYC | 1 cut(s) | 1091 |
| Eco32I | GATATC | 3 cut(s) | 41, 1249, 1384 |
| Eco47I | GGWCC | 2 cut(s) | 644, 950 |
| Eco53kI | GAGCTC | 1 cut(s) | 1089 |
| Eco57I | CTGAAG | 1 cut(s) | 2874 |
| Eco81I | CCTNAGG | 2 cut(s) | 734, 1742 |
| Eco88I | CYCGRG | 2 cut(s) | 1838, 2275 |
| EcoICRI | GAGCTC | 1 cut(s) | 1089 |
| EcoRII | CCWGG | 3 cut(s) | 952, 1852, 2575 |
| EcoRV | GATATC | 3 cut(s) | 41, 1249, 1384 |
| EcoT14I | CCWWGG | 2 cut(s) | 1505, 2687 |
| EcoT22I | ATGCAT | 2 cut(s) | 1765, 2872 |
| EcoT38I | GRGCYC | 1 cut(s) | 1091 |
| ErhI | CCWWGG | 2 cut(s) | 1505, 2687 |
| Esp3I | CGTCTC | 1 cut(s) | 2088 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 767, 799, 780, 812 |
| FaqI | GGGAC | 2 cut(s) | 488, 2769 |
| FauNDI | CATATG | 1 cut(s) | 2055 |
| FbaI | TGATCA | 1 cut(s) | 139 |
| FblI | GTMKAC | 4 cut(s) | 691, 1159, 1480, 2281 |
| Fnu4HI | GCNGC | 5 cut(s) | 1865, 2377, 2693, 2863, 2974 |
| FokI | GGATG | 6 cut(s) | 269, 299, 732, 1451, 1772, 2121 |
| FriOI | GRGCYC | 1 cut(s) | 1091 |
| Fsp4HI | GCNGC | 5 cut(s) | 1865, 2377, 2693, 2863, 2974 |
| GlaI | GCGC | 3 cut(s) | 177, 782, 2896 |
| GluI | GCNGC | 5 cut(s) | 1865, 2377, 2693, 2863, 2974 |
| GsuI | CTGGAG | 2 cut(s) | 375, 2471 |
| HaeII | RGCGCY | 1 cut(s) | 2898 |
| HaeIII | GGCC | 1 cut(s) | 1852 |
| HapII | CCGG | 5 cut(s) | 243, 1572, 1834, 2808, 2960 |
| HgaI | GACGC | 2 cut(s) | 2070, 2662 |
| HhaI | GCGC | 3 cut(s) | 178, 783, 2897 |
| Hin1I | GRCGYC | 2 cut(s) | 72, 2081 |
| Hin6I | GCGC | 3 cut(s) | 176, 781, 2895 |
| HinP1I | GCGC | 3 cut(s) | 176, 781, 2895 |
| HindIII | AAGCTT | 3 cut(s) | 82, 426, 1112 |
| HpaII | CCGG | 5 cut(s) | 243, 1572, 1834, 2808, 2960 |
| HphI | GGTGA | 3 cut(s) | 1355, 2491, 2531 |
| Hpy166II | GTNNAC | 7 cut(s) | 692, 1160, 1283, 1411, 1481, 1675, 2282 |
| Hpy188I | TCNGA | 7 cut(s) | 70, 337, 520, 1042, 1518, 1962, 2171 |
| Hpy8I | GTNNAC | 7 cut(s) | 692, 1160, 1283, 1411, 1481, 1675, 2282 |
| Hpy99I | CGWCG | 2 cut(s) | 1518, 2981 |
| HpyAV | CCTTC | 5 cut(s) | 1047, 1889, 1906, 2372, 2745 |
| HpyCH4III | ACNGT | 6 cut(s) | 108, 313, 2118, 2550, 2733, 2760 |
| HpyCH4IV | ACGT | 5 cut(s) | 72, 1096, 1162, 1513, 2719 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 997, 1364, 2657, 2666, 2692, 2982 |
| HpySE526I | ACGT | 5 cut(s) | 72, 1096, 1162, 1513, 2719 |
| Hsp92I | GRCGYC | 2 cut(s) | 72, 2081 |
| HspAI | GCGC | 3 cut(s) | 176, 781, 2895 |
| KpnI | GGTACC | 1 cut(s) | 1572 |
| KroI | GCCGGC | 1 cut(s) | 1833 |
| KroNI | GCCGGC | 1 cut(s) | 1835 |
| Ksp22I | TGATCA | 1 cut(s) | 139 |
| LguI | GCTCTTC | 2 cut(s) | 1096, 1494 |
| LmnI | GCTCC | 4 cut(s) | 420, 1152, 1574, 2700 |
| Lsp1109I | GCAGC | 5 cut(s) | 1851, 2363, 2704, 2849, 2960 |
| LweI | GCATC | 7 cut(s) | 1519, 1750, 2099, 2204, 2420, 2660, 2857 |
| MaeII | ACGT | 5 cut(s) | 72, 1096, 1162, 1513, 2719 |
| MaeIII | GTNAC | 7 cut(s) | 532, 970, 1126, 1461, 1746, 2458, 2740 |
| MflI | RGATCY | 8 cut(s) | 204, 328, 1059, 1518, 1924, 1957, 2035, 2293 |
| MhlI | GDGCHC | 1 cut(s) | 1091 |
| MlsI | TGGCCA | 1 cut(s) | 1852 |
| MluNI | TGGCCA | 1 cut(s) | 1852 |
| MlyI | GAGTC | 4 cut(s) | 34, 1275, 2287, 2415 |
| MmeI | TCCRAC | 4 cut(s) | 246, 1496, 2504, 2569 |
| Mox20I | TGGCCA | 1 cut(s) | 1852 |
| Mph1103I | ATGCAT | 2 cut(s) | 1765, 2872 |
| MroNI | GCCGGC | 1 cut(s) | 1833 |
| MroXI | GAANNNNTTC | 2 cut(s) | 795, 2049 |
| MscI | TGGCCA | 1 cut(s) | 1852 |
| MslI | CAYNNNNRTG | 4 cut(s) | 843, 875, 1290, 1758 |
| Msp20I | TGGCCA | 1 cut(s) | 1852 |
| MspA1I | CMGCKG | 1 cut(s) | 1864 |
| MspI | CCGG | 5 cut(s) | 243, 1572, 1834, 2808, 2960 |
| MspR9I | CCNGG | 5 cut(s) | 244, 954, 1854, 2577, 2809 |
| Mva1269I | GAATGC | 1 cut(s) | 1458 |
| MvaI | CCWGG | 3 cut(s) | 954, 1854, 2577 |
| MvnI | CGCG | 1 cut(s) | 2660 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 997, 1364, 2657, 2666, 2692, 2982 |
| NaeI | GCCGGC | 1 cut(s) | 1835 |
| NciI | CCSGG | 2 cut(s) | 244, 2809 |
| NdeI | CATATG | 1 cut(s) | 2055 |
| NgoMIV | GCCGGC | 1 cut(s) | 1833 |
| NlaIV | GGNNCC | 2 cut(s) | 422, 1570 |
| NmuCI | GTSAC | 2 cut(s) | 970, 2740 |
| NsiI | ATGCAT | 2 cut(s) | 1765, 2872 |
| NspI | RCATGY | 2 cut(s) | 387, 2571 |
| NspV | TTCGAA | 1 cut(s) | 2815 |
| PaeI | GCATGC | 1 cut(s) | 2571 |
| PaeR7I | CTCGAG | 2 cut(s) | 1838, 2275 |
| PciI | ACATGT | 1 cut(s) | 383 |
| PciSI | GCTCTTC | 2 cut(s) | 1096, 1494 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 69 |
| PctI | GAATGC | 1 cut(s) | 1458 |
| PdiI | GCCGGC | 1 cut(s) | 1835 |
| PdmI | GAANNNNTTC | 2 cut(s) | 795, 2049 |
| PfeI | GAWTC | 6 cut(s) | 147, 197, 1662, 2005, 2444, 2812 |
| PfoI | TCCNGGA | 1 cut(s) | 952 |
| PkrI | GCNGC | 5 cut(s) | 1866, 2378, 2694, 2864, 2975 |
| PleI | GAGTC | 4 cut(s) | 33, 1274, 2286, 2415 |
| PpsI | GAGTC | 4 cut(s) | 33, 1274, 2286, 2415 |
| Ppu21I | YACGTR | 1 cut(s) | 1163 |
| PscI | ACATGT | 1 cut(s) | 383 |
| PshBI | ATTAAT | 1 cut(s) | 1611 |
| Psp124BI | GAGCTC | 1 cut(s) | 1091 |
| Psp6I | CCWGG | 3 cut(s) | 952, 1852, 2575 |
| PspGI | CCWGG | 3 cut(s) | 952, 1852, 2575 |
| PspN4I | GGNNCC | 2 cut(s) | 422, 1570 |
| PspPI | GGNCC | 2 cut(s) | 644, 950 |
| PspXI | VCTCGAGB | 2 cut(s) | 1838, 2275 |
| PstI | CTGCAG | 2 cut(s) | 652, 2381 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1867 |
| PsuI | RGATCY | 8 cut(s) | 204, 328, 1059, 1518, 1924, 1957, 2035, 2293 |
| PvuII | CAGCTG | 1 cut(s) | 1864 |
| RsaI | GTAC | 7 cut(s) | 31, 79, 667, 1260, 1570, 1844, 2099 |
| RsaNI | GTAC | 7 cut(s) | 30, 78, 666, 1259, 1569, 1843, 2098 |
| RseI | CAYNNNNRTG | 4 cut(s) | 843, 875, 1290, 1758 |
| SacI | GAGCTC | 1 cut(s) | 1091 |
| SapI | GCTCTTC | 2 cut(s) | 1096, 1494 |
| SatI | GCNGC | 5 cut(s) | 1865, 2377, 2693, 2863, 2974 |
| Sau96I | GGNCC | 2 cut(s) | 644, 950 |
| SbfI | CCTGCAGG | 1 cut(s) | 652 |
| ScaI | AGTACT | 1 cut(s) | 1260 |
| SchI | GAGTC | 4 cut(s) | 34, 1275, 2287, 2415 |
| ScrFI | CCNGG | 5 cut(s) | 244, 954, 1854, 2577, 2809 |
| SdaI | CCTGCAGG | 1 cut(s) | 652 |
| SduI | GDGCHC | 1 cut(s) | 1091 |
| SfaNI | GCATC | 7 cut(s) | 1519, 1750, 2099, 2204, 2420, 2660, 2857 |
| SfcI | CTRYAG | 2 cut(s) | 648, 2377 |
| Sfr274I | CTCGAG | 2 cut(s) | 1838, 2275 |
| SfuI | TTCGAA | 1 cut(s) | 2815 |
| SinI | GGWCC | 2 cut(s) | 644, 950 |
| SlaI | CTCGAG | 2 cut(s) | 1838, 2275 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 843, 875, 1290, 1758 |
| SmlI | CTYRAG | 8 cut(s) | 151, 183, 1533, 1838, 1877, 2087, 2252, 2275 |
| SmoI | CTYRAG | 8 cut(s) | 151, 183, 1533, 1838, 1877, 2087, 2252, 2275 |
| SpeI | ACTAGT | 1 cut(s) | 1471 |
| SphI | GCATGC | 1 cut(s) | 2571 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 652 |
| SsiI | CCGC | 3 cut(s) | 343, 1799, 2936 |
| SstI | GAGCTC | 1 cut(s) | 1091 |
| StyD4I | CCNGG | 5 cut(s) | 242, 952, 1852, 2575, 2807 |
| StyI | CCWWGG | 2 cut(s) | 1505, 2687 |
| TaaI | ACNGT | 6 cut(s) | 108, 313, 2118, 2550, 2733, 2760 |
| TaiI | ACGT | 5 cut(s) | 75, 1099, 1165, 1516, 2722 |
| TatI | WGTACW | 2 cut(s) | 1258, 2097 |
| TfiI | GAWTC | 6 cut(s) | 147, 197, 1662, 2005, 2444, 2812 |
| TscAI | CASTG | 2 cut(s) | 2454, 2992 |
| TseFI | GTSAC | 2 cut(s) | 970, 2740 |
| TseI | GCWGC | 5 cut(s) | 1864, 2376, 2692, 2862, 2973 |
| Tsp45I | GTSAC | 2 cut(s) | 970, 2740 |
| TspGWI | ACGGA | 6 cut(s) | 65, 78, 1234, 1451, 2763, 2994 |
| TspRI | CASTG | 2 cut(s) | 2454, 2992 |
| VpaK11BI | GGWCC | 2 cut(s) | 644, 950 |
| VspI | ATTAAT | 1 cut(s) | 1611 |
| XapI | RAATTY | 7 cut(s) | 588, 1790, 1888, 1942, 2494, 2620, 2631 |
| XceI | RCATGY | 2 cut(s) | 387, 2571 |
| XhoI | CTCGAG | 2 cut(s) | 1838, 2275 |
| XmaJI | CCTAGG | 1 cut(s) | 2687 |
| XmiI | GTMKAC | 4 cut(s) | 691, 1159, 1480, 2281 |
| XmnI | GAANNNNTTC | 2 cut(s) | 795, 2049 |
| ZraI | GACGTC | 1 cut(s) | 73 |
| ZrmI | AGTACT | 1 cut(s) | 1260 |
| Zsp2I | ATGCAT | 2 cut(s) | 1765, 2872 |